CN105168359A - Novel application of radix fici simplicissimae and extract thereof - Google Patents
Novel application of radix fici simplicissimae and extract thereof Download PDFInfo
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- CN105168359A CN105168359A CN201510546810.1A CN201510546810A CN105168359A CN 105168359 A CN105168359 A CN 105168359A CN 201510546810 A CN201510546810 A CN 201510546810A CN 105168359 A CN105168359 A CN 105168359A
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- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 claims abstract description 34
- 230000008021 deposition Effects 0.000 claims abstract description 8
- 101710147108 Tyrosinase inhibitor Proteins 0.000 claims abstract description 7
- 239000002537 cosmetic Substances 0.000 claims abstract description 5
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Abstract
The invention relates to the technical field of traditional Chinese medicine, and discloses application of radix fici simplicissimae and an extract thereof in preparing a tyrosinase inhibitor, a melanogenesis inhibitor, whitening cosmetics as well as in skin whitening and skin melanin deposition prevention. The radix fici simplicissimae and the extract thereof are excellent in tyrosinase inhibiting activity, and especially the radix fici simplicissimae extract is more significant in tyrosinase inhibiting activity compared with existing tyrosinase inhibitors and arbutin which serves as a whitening agent; and meanwhile, the radix fici simplicissimae and the extract thereof are effective in preventing melanin deposition and are relatively high in radical scavenging rate, and the radix fici simplicissimae and the extract thereof are relatively high in content of total flavonoids and are better applicable to the fields of beautifying and whitening.
Description
Technical field
The present invention relates to technical field of Chinese medicines, relate to the new opplication of Herba Fici Simplicissimae and extract thereof specifically.
Background technology
The colour of skin is that people examine one of standard of beauty.For Asia women, the pale colour of skin especially one is generally pursued.For this reason, various product arises at the historic moment, as: whitening products, anti-ultraviolet product and film product etc.After deliberation, skin color is comprehensively determined by various pigments contained in Skin Cell.In numerous pigment, melanin amount contained in melanocyte is topmost determiner.Further research confirms, tryrosinase (EC1.14.18.1) is the key enzyme of B16 cell.Thus, active by restraint of tyrosinase, generate to reduce melanin, thus the skin effect reaching whitening becomes one of the important evidence of research and development skin-protection product (Chinese journals of practical medicine [J], 2005,21,1364-1366; Xiamen University's journal (natural science edition) [J], 2007,46,274-282).Whitening agent conventional in these products as: hydroquinone, kojic acid, arbutin etc. are several, and these materials more or less have suitable cytotoxicity.Therefore, the safety of tyrosinase inhibitor needs the factor considered of laying equal stress in whitening and skin-protecting R & D of complex, and the selection of Chinese herbal medicine is easy to be esthetically acceptable to the consumers undoubtedly in this respect more.
Herba Fici Simplicissimae, another name Ficus hirta, Herba Ipomoeae Cairicae, milk root, southern stilbene, there are medicinal record in multiple nationalitys such as Guangxi, Guangdong, Yunnan, and the ground such as main product Guangxi, Guangdong, are described as " panacis quinquefolii radix ".Herba Fici Simplicissimae is the root of Moraceae ficus species ficus simplicissima (Ficushirtavahl.).Herba Fici Simplicissimae for health care, has spleen invigorating to dry among the people, effect of relieving cough and resolving phlegm, dredge the meridian passage, the use history having more than one thousand years among the people in Guangdong.(the number of patent application: 200510002111.7) disclose the novelty teabag of Herba Fici Simplicissimae in preparation hepatitis adjuvant therapy medicaments and health food such as the Zhang Baoxu of Peking University.The Yuan Lixia (application number: 201010274140.X) of Nanfang Medical Univ discloses a kind of pharmaceutical composition preventing and treating viral myocarditis and preparation method thereof.Wang Xiao equality (Wang Xiao equality, the experimentation of Herba Fici Simplicissimae Aqueous extracts vitro antibacterial activity, time precious traditional Chinese medical science traditional Chinese medicines, 2010,21 (7): 1692-1693.) report Herba Fici Simplicissimae Aqueous extracts all inhibited to escherichia coli, bacillus subtilis, staphylococcus aureus, minimum inhibitory concentration is 1g/ml.In addition report Radix Fici Simplicissimae extract is also had to have anti-inflammatory, anti-acid and aging-resistant effect.But Herba Fici Simplicissimae is mainly applied and is only limitted to above-mentioned several aspect at present, deep research report also be yet there are no to other using value of its extract.
Chinese patent CN103877283A discloses the herb liquid of a kind of whitening and speckle dispelling, eliminating toxin and beautifying the skin, and it obtains with the 10 kinds of Chinese medicines composition compound recipe decoctings comprising Herba Fici Simplicissimae, can restraint of tyrosinase active, there is effect of whitening and speckle dispelling and eliminating toxin and beautifying the skin.But the realization of this effect is based upon on whole Chinese medicine compound combined effect basis, this patent unexposed Herba Fici Simplicissimae or Radix Fici Simplicissimae extract separately can restraint of tyrosinase active, and there is the effect of skin whitening.
There is not the record of beautifying whitening related efficacy in Herba Fici Simplicissimae, have no the purposes of Herba Fici Simplicissimae as tyrosinase inhibitor, and Herba Fici Simplicissimae is applied to the report of skin-lightening cosmetic in prior art in Chinese medicine is recorded yet.
Summary of the invention
In view of this, the object of the present invention is to provide Herba Fici Simplicissimae and extract thereof in the relevant application of a series of beautifying whitening, be mainly included in prepare tyrosinase inhibitor, Melanin inhibitor, skin-lightening cosmetic and skin-whitening, the new opplication prevented in melanin deposition.
At the field of Chinese medicines, effect of Herba Fici Simplicissimae is recorded and is only limitted to the merit with strengthening the spleen and tonifying the lung, circulation of qi promoting dampness removing, relaxing muscles and tendons and activating QI and blood in the collateral, the traditional Chinese medical science of Lingnan area or ethnic groups doctor among the people are usually used in treating that insufficiency of the spleen edema, lack of appetite are unable, pulmonary tuberculosis cough, night sweat, leukorrhagia, puerperal agalactia, menoxenia, rheumatic arthralgia, the disease such as edema.And the present invention finds through creative research, Radix Fici Simplicissimae extract has the ability of restraint of tyrosinase activity preferably, can be used in the preparation of skin-whitening and relevant cosmetics.
As preferably, described Radix Fici Simplicissimae extract is its water extract or ethanol extract.
More preferably, Radix Fici Simplicissimae extract of the present invention obtains by the following method:
Step 1, Herba Fici Simplicissimae add ethanol water and extract after pulverizing, except desolventizing obtains Herba Fici Simplicissimae crude extract after extracting;
Step 2, Herba Fici Simplicissimae crude extract add deionized water dissolving, then loading is to macroporous adsorbent resin, gradient elution is carried out successively with deionized water, gradient concentration alcoholic solution from low to high, collect the eluent of 60v/v%-95v/v% concentration ethanol eluting wherein respectively, removed under reduced pressure second alcohol and water, obtains Radix Fici Simplicissimae extract.
Radix Fici Simplicissimae extract of the present invention can be the eluent from single concentration ethanol eluting each in 60v/v%-95v/v% concentration, also can be their mixture.
In certain embodiments of the present invention, the eluent collecting 60v/v%-95v/v% concentration ethanol eluting wherein in step 2 respectively can be eluent (also can be described as 60v/v% alcohol elution, below roughly the same), the eluent of 80v/v% ethanol elution, the eluent of 90v/v% ethanol elution, the eluent of 95v/v% ethanol elution of 60v/v% ethanol elution.
As preferably, be extracted as supersound extraction described in step 1, stir and extract or circulated in countercurrent extraction.
As preferably, gradient concentration alcoholic solution from low to high described in step 2 is the gradient concentration alcoholic solution of 20v/v%-95v/v% concentration.More preferably, gradient concentration alcoholic solution from low to high described in described step 2 is the gradient concentration alcoholic solution be made up of 20v/v% alcoholic solution, 30v/v% alcoholic solution, 40v/v% alcoholic solution, 60v/v% alcoholic solution, 80v/v% alcoholic solution, 90v/v% alcoholic solution and 95v/v% alcoholic solution.In addition, in detailed description of the invention more of the present invention, described gradient concentration alcoholic solution from low to high can also one of following be selected:
(1) 20v/v% alcoholic solution, 40v/v% alcoholic solution, 60v/v% alcoholic solution, 80v/v% alcoholic solution, 95v/v% alcoholic solution;
(2) 40v/v% alcoholic solution, 60v/v% alcoholic solution, 80v/v% alcoholic solution;
(3) 30v/v% alcoholic solution, 60v/v% alcoholic solution, 90v/v% alcoholic solution.
As preferably, described macroporous adsorbent resin is AB-8 type macroporous adsorbent resin, D301 type macroporous adsorbent resin or X-5 type macroporous adsorbent resin.
As preferably, the extraction time of extracting described in step 1 is 0.5-2h, in detailed description of the invention more of the present invention, can select 0.5h, 1h or 2h.
As preferably, in step 1, the mass volume ratio of Herba Fici Simplicissimae and ethanol water is 1g:10-20mL, in detailed description of the invention more of the present invention, can represent with 40g and 400mL, 40g and 800mL, 20g and 400mL.
As preferably, the concentration of alcohol of ethanol water described in step 1 is 50v/v%-90v/v%, in detailed description of the invention more of the present invention, can select 50v/v%, 70v/v% or 90v/v%.
As preferably, the general flavone content of described Radix Fici Simplicissimae extract is 7.9-17.5%.
Preparation method of the present invention is obtained Radix Fici Simplicissimae extract compare with existing tyrosinase inhibitor and whitening agent arbutin, the tyrosinase inhibition rate of arbutin is 43%, and the tyrosinase inhibition rate of Radix Fici Simplicissimae extract of the present invention is at 45-69%, apparently higher than the inhibition of existing product arbutin.
Meanwhile, containing more total flavones in Radix Fici Simplicissimae extract of the present invention, and in scavenging free radicals effect, Radix Fici Simplicissimae extract of the present invention has the free radical scavenging activity up to 65-77%, can apply in whitening product.
In addition, Radix Fici Simplicissimae extract of the present invention is acted on zebrafish embryo, result shows, and Radix Fici Simplicissimae extract of the present invention generates Brachydanio rerio melanin has inhibit activities, can prevent skin melanin deposition.Preferably, the Radix Fici Simplicissimae extract with melanin generation inhibit activities is the 80v/v% concentration ethanol eluting position obtained according to Radix Fici Simplicissimae extract preparation method of the present invention.
Radix Fici Simplicissimae extract of the present invention both can be applied in each application of the present invention as active substance separately, and other active substances also can be coordinated together to use.
From above technical scheme, Herba Fici Simplicissimae and extract thereof have excellent tyrosinase inhibitory activity, particularly Radix Fici Simplicissimae extract has more significant tyrosine inhibit activities compared with existing tyrosinase inhibitor and whitening agent arbutin, have simultaneously and prevent melanin deposition effect and higher free radical scavenging activity, containing more total flavones, can be applied to better in beautifying whitening field.
Accompanying drawing explanation
Figure 1 shows that the microscopy figure of zebrafish embryo apparent black element inhibition; Wherein 1-A represents blank microscopy result; 1-B is the microscopy result adopting the process of Herba Fici Simplicissimae 80v/v% alcohol elution.
Detailed description of the invention
The embodiment of the invention discloses the new opplication of Herba Fici Simplicissimae and extract thereof.Those skilled in the art can use for reference present disclosure, and suitable improving technique parameter realizes.Special needs to be pointed out is, all similar replacements and change apparent to those skilled in the art, they are all deemed to be included in the present invention.Application of the present invention is described by preferred embodiment, related personnel obviously can not depart from content of the present invention, spirit and scope product as herein described and method are changed or suitably change with combination, realize and apply the technology of the present invention.
In order to understand the present invention further, be described in detail below in conjunction with the new opplication of embodiment to Herba Fici Simplicissimae provided by the invention and extract thereof.
Embodiment 1: the preparation of Radix Fici Simplicissimae extract of the present invention
By 40g Herba Fici Simplicissimae crushed after being dried, mix with 400mL70v/v% alcoholic solution, supersound extraction 0.5 hour, isolates crude extract, after removed under reduced pressure solvent, obtains Herba Fici Simplicissimae crude extract 2.6g.
Get Herba Fici Simplicissimae crude extract 1.6g, after deionized water dissolving, be loaded to AB-8 resin filling post, use deionized water, 20v/v% ethanol, 40v/v% ethanol, 60v/v% ethanol, 80v/v% ethanol and 95v/v% ethanol gradient elution successively, collect the eluent of 60v/v% ethanol, 80v/v% ethanol, 95v/v% ethanol respectively, removed under reduced pressure second alcohol and water, obtains Radix Fici Simplicissimae extract, freezen protective at collecting-20 DEG C.
Embodiment 2: the preparation of Radix Fici Simplicissimae extract of the present invention
By 20g Herba Fici Simplicissimae crushed after being dried, mix with 400mL50v/v% alcoholic solution, stir extraction 2 hours, isolate crude extract, after removed under reduced pressure solvent, obtain Herba Fici Simplicissimae crude extract 1.7g.
Get Herba Fici Simplicissimae crude extract 1.0g, after deionized water dissolving, be loaded to D301 resin filling post, use deionized water, 40v/v% ethanol, 60v/v% ethanol, 80v/v% ethanol gradient elution successively, collect the eluent of 60v/v% ethanol, 80v/v% ethanol respectively, removed under reduced pressure second alcohol and water, obtains Radix Fici Simplicissimae extract, freezen protective at collecting-20 DEG C.
Embodiment 3: the preparation of Radix Fici Simplicissimae extract of the present invention
By 40g Herba Fici Simplicissimae crushed after being dried, mix with 800mL90v/v% alcoholic solution, circulated in countercurrent extracts 1 hour, isolates crude extract, after removed under reduced pressure solvent, obtains Herba Fici Simplicissimae crude extract 1.9g.
Get Herba Fici Simplicissimae crude extract 1.5g, after deionized water dissolving, be loaded to X-5 resin filling post, use deionized water, 30v/v% ethanol, 60v/v% ethanol, 90v/v% ethanol gradient elution successively, collect the eluent of 60v/v% ethanol, 90v/v% ethanol respectively, removed under reduced pressure second alcohol and water, obtains Radix Fici Simplicissimae extract, freezen protective at collecting-20 DEG C.
Embodiment 4: determination of total flavonoids in Radix Fici Simplicissimae extract
Instrument: generally analyse general T6 ultraviolet-visual spectrometer,
Working sample: extract of the present invention is the Radix Fici Simplicissimae extract of 60v/v% alcohol elution in embodiment 1; The Radix Fici Simplicissimae extract of 80v/v% alcohol elution, 95v/v% alcohol elution;
Reference extract be according to embodiment 1 method collect Herba Fici Simplicissimae crude extract, washing the Radix Fici Simplicissimae extract at position, the Radix Fici Simplicissimae extract of 20v/v% alcohol elution and 40v/v% alcohol elution Radix Fici Simplicissimae extract;
Determination step: according to Pharmacopoeia of People's Republic of China (2010 editions) method take rutin as standard substance, measures general flavone content in Radix Fici Simplicissimae extract.Determination step is: 1) standard curve making: precision measures reference substance solution 1ml, 2ml, 3ml, 4ml, 5ml, 6ml, puts in 25ml measuring bottle respectively, respectively adds 30% ethanol to 6.0ml, adds 5% sodium nitrite solution 1ml.Mixing, places 6 minutes, then adds 10% aluminum nitrate solution 1ml, shake up, place 6 minutes.Hydro-oxidation sodium test solution 10ml, then add 30% ethanol to scale, shake up, place 15 minutes, take corresponding reagent as blank, with ultraviolet visible spectrophotometry, measure absorbance at the wavelength place of 510nm, take absorbance as vertical coordinate, concentration is abscissa, drawing standard curve.2) get a certain amount of sample and be placed in 25ml volumetric flask, other is according to standard curve preparation process, from " adding sodium nitrite solution 1ml ", measure absorbance in accordance with the law, get need testing solution 3ml simultaneously, except not hydro-oxidation sodium test solution, all the other the same operations, as blank, calculate general flavone content (in rutin) in Radix Fici Simplicissimae extract.Determination of total flavonoids result is as shown in table 1.
General flavone content in table 1 Radix Fici Simplicissimae extract
| Sample | General flavone content % |
| Herba Fici Simplicissimae crude extract | 5.1 |
| Water elution position Radix Fici Simplicissimae extract | 0.3 |
| 20v/v% alcohol elution Radix Fici Simplicissimae extract | 1.4 |
| 40v/v% alcohol elution Radix Fici Simplicissimae extract | 3.6 |
| 60v/v% alcohol elution Radix Fici Simplicissimae extract | 8.8 |
| 80v/v% alcohol elution Radix Fici Simplicissimae extract | 17.5 |
| 95v/v% alcohol elution Radix Fici Simplicissimae extract | 7.9 |
Can find out according to the above results, prepare by the method for the invention the total flavones that Radix Fici Simplicissimae extract has high-load.
Embodiment 5: the detection of tyrosinase inhibitory activity
Test sample: the Radix Fici Simplicissimae extract that the Herba Fici Simplicissimae crude extract prepared according to embodiment 1, embodiment 2 and embodiment 3 method and different concentration ethanol eluting position obtain, this had wherein both included Radix Fici Simplicissimae extract of the present invention, also included the Radix Fici Simplicissimae extract of contrast.
Above-mentioned each sample being dissolved in deionized water, being mixed with aqueous solution, for measuring tyrosinase inhibitory activity, take arbutin as object of reference.Determination step is: soda acid buffer solution (pH6.8,30mM) 70 μ L, 1.66mM tyrosine solution 80 μ L, sample solution 80 μ L mix homogeneously, incubation more than 5 minutes in 37 DEG C of thermostatic water bath, add tyrosinase solution (125U/ml) 10 μ L, after 37 DEG C of constant temperature 10min, measure the absorbance A of 475nm wavelength by microplate reader
475.Replace sample aqueous solution with deionized water, measure absorbance equally as reference solution, the results are shown in Table 2.
Inhibitory activity against tyrosinase computing formula is: suppression ratio (%)=(A
0-(A
475-B))/A
0× 100%, wherein A
0the absorbance of reference solution, A
475be the absorbance of sample solution, B is the absorbance of sample blank solution.
The measurement result of table 2 tyrosinase inhibitory activity
| Sample | Sample solution concentration mg/ml | Tyrosinase inhibition rate % |
| Embodiment 1 (Herba Fici Simplicissimae crude extract) | 1.0 | 25 |
| Embodiment 1 (water elution position) | 1.0 | 15 |
| Embodiment 1 (20v/v% alcohol elution) | 1.0 | 11 |
| Embodiment 1 (40v/v% alcohol elution) | 1.0 | 22 |
| Embodiment 1 (60v/v% alcohol elution) | 1.0 | 57 |
| Embodiment 1 (80v/v% alcohol elution) | 0.5 | 57 |
| Embodiment 1 (95v/v% alcohol elution) | 0.5 | 45 |
| Embodiment 2 (30v/v% alcohol elution) | 1.0 | 27 |
| Embodiment 2 (60v/v% alcohol elution) | 1.0 | 45 |
| Embodiment 2 (90v/v% alcohol elution) | 1.0 | 62 |
| Embodiment 3 (40v/v% alcohol elution) | 1.0 | 15 |
| Embodiment 3 (60v/v% alcohol elution) | 1.0 | 56 |
| Embodiment 3 (80v/v% alcohol elution) | 1.0 | 69 |
| Arbutin | 1.0 | 43.0 |
From table 2, the crude extract that Herba Fici Simplicissimae obtains after extracting has the inhibitory action to tyrosinase activity, after adsorbent resin is separated, gained 60v/v% ethanol, 80v/v% ethanol, 90v/v% ethanol and 95v/v% alcohol elution all can realize the suppression to tyrosinase activity, and higher than the inhibitory action of crude extract and arbutin.Wherein the inhibitory action of 80v/v% alcohol elution to tyrosinase activity is the strongest.
Embodiment 6: the detection of Free-radical scavenging activity
Test sample: the Radix Fici Simplicissimae extract that the Herba Fici Simplicissimae crude extract prepared according to embodiment 1, embodiment 2 and embodiment 3 method and different concentration ethanol eluting position obtain, this had wherein both included Radix Fici Simplicissimae extract of the present invention, also included the Radix Fici Simplicissimae extract of contrast.
Its Free-radical scavenging activity is measured, with V by DPPH method
cfor object of reference.Determination step is: accurately pipette extracting solution 2mL in 10mL tool plug test tube, add people 2mLDPPH alcoholic solution (2 × 10
-4mol/L), fully mix, room temperature leaves standstill, by the absorbance A of spectrophotometric determination 517nm wavelength after 30min
517(taking ethanol as reference); Simultaneously test 2mLDPPH solution and the mixed absorbance A of 2mL ethanol
c, and 2mL extracting solution and the mixed absorbance A of 2mL alcoholic solution
0.Parallel assay three times, averages, according to following formulae discovery free radical scavenging activity.
Free radical scavenging activity=(1-(A
517-A
0)/A
c) × 100%.
The testing result of table 3DPPH method Free-radical scavenging activity
From table 3, the crude extract that Herba Fici Simplicissimae obtains after extracting, the scavenging action to DPPH free radical can be realized, after adsorbent resin is separated, gained 60v/v% ethanol, 80v/v% ethanol and 95v/v% alcohol elution all can realize the removing to DPPH free radical, and improve a lot compared with the scavenging action of crude extract, wherein the scavenging action of 80v/v% alcohol elution to free radical is the strongest.
Embodiment 7: Radix Fici Simplicissimae extract suppresses zebrafish embryo melanin to produce experiment
Herba Fici Simplicissimae 80v/v% alcohol elution embodiment 1 prepared, is dissolved in deionized water, is mixed with the aqueous solution of 10mg/ml, for measuring the inhibitory action generated zebrafish embryo melanin.Determination step is as follows: the raising of Brachydanio rerio and the collection of embryo, Adult Zebrafish is raised in laboratory circulating water culture system, 28.5 DEG C, light/dark cycle is 14/10 hour, raising water filters and abundant aeration through blood circulation, pH value keep about 8.0, every day feeding fairy shrimp three times, feeding 6 days weekly.Selection form is normal, and individual larger and healthy sexual maturity Brachydanio rerio, carries out pairing at hatching system by sex ration 1:2 and raise in 15L10-15 bar glass aquarium.In 22:00 light-off evening before that day of laying eggs, morning next day, 8:00 system was turned on light automatically, started to lay eggs under light stimulation, collected embryo.The embryo of collection is cleaned fully after removing foul, pick out the normal embryo of healthy development under the microscope, using 96 orifice plates as test chamber, in every hole, add Radix Fici Simplicissimae extract aqueous solution respectively, be assigned randomly in 96 orifice plates by 1 embryo/hole.Change Radix Fici Simplicissimae extract aqueous solution to be measured every day and stir, to guarantee that active component is evenly distributed.Pigmentation based on phenotype is assessed, and observe the melanin inhibition of zebrafish embryo, the result detected is as shown in table 4, and zebrafish embryo microscope collection of illustrative plates as shown in Figure 1.
Table 4 zebrafish embryo melanin inhibition
As shown in Table 4, Radix Fici Simplicissimae extract 80% ethanol position has the inhibitory action generated zebrafish embryo melanin, high-visible in the microscopy result of simultaneously Fig. 1, a large amount of melanin depositions is there is in blank, and through the microscopy result of Radix Fici Simplicissimae extract process without any melanin deposition.
The explanation of above embodiment just understands method of the present invention and core concept thereof for helping.It should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention, can also carry out some improvement and modification to the present invention, these improve and modify and also fall in the protection domain of the claims in the present invention.
Claims (10)
1. Herba Fici Simplicissimae and extract thereof preparing tyrosinase inhibitor, Melanin inhibitor, skin-lightening cosmetic and skin-whitening, the application prevented in skin melanin deposition.
2. apply according to claim 1, it is characterized in that, described Radix Fici Simplicissimae extract is its water extract or ethanol extract.
3. apply according to claim 1 or 2, it is characterized in that, described Radix Fici Simplicissimae extract is prepared by following methods:
Step 1, Herba Fici Simplicissimae add ethanol water and extract after pulverizing, except desolventizing obtains Herba Fici Simplicissimae crude extract after extracting;
Step 2, Herba Fici Simplicissimae crude extract add deionized water dissolving, then loading is to macroporous adsorbent resin, gradient elution is carried out successively with deionized water, gradient concentration alcoholic solution from low to high, collect the eluent of 60v/v%-95v/v% concentration ethanol eluting wherein respectively, removed under reduced pressure second alcohol and water, obtains Radix Fici Simplicissimae extract.
4. apply according to claim 3, it is characterized in that, described in step 1, be extracted as supersound extraction, stir and extract or circulated in countercurrent extraction.
5. apply according to claim 3, it is characterized in that, gradient concentration alcoholic solution from low to high described in step 2 is the gradient concentration alcoholic solution of 20v/v%-95v/v% concentration.
6. apply according to claim 5, it is characterized in that, gradient concentration alcoholic solution from low to high described in described step 2 is the gradient concentration alcoholic solution be made up of 20v/v% alcoholic solution, 30v/v% alcoholic solution, 40v/v% alcoholic solution, 60v/v% alcoholic solution, 80v/v% alcoholic solution, 90v/v% alcoholic solution and 95v/v% alcoholic solution.
7. apply according to claim 3, it is characterized in that, described macroporous adsorbent resin is AB-8 type macroporous adsorbent resin, D301 type macroporous adsorbent resin or X-5 type macroporous adsorbent resin.
8. apply according to claim 3, it is characterized in that, the extraction time of extracting described in step 1 is 0.5-2h.
9. apply according to claim 3, it is characterized in that, in step 1, the mass volume ratio of Herba Fici Simplicissimae and ethanol water is 1g:10-20mL.
10. apply according to claim 3, it is characterized in that, the general flavone content of described Radix Fici Simplicissimae extract is 7.9-17.5%.
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| CN115054554A (en) * | 2022-07-20 | 2022-09-16 | 三明学院 | Hispid fig washing-free hand sanitizer and preparation method thereof |
| CN115089629A (en) * | 2022-07-20 | 2022-09-23 | 三明学院 | Freeze-dried hispid fig fruit flavone extracting solution and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109602669A (en) * | 2019-02-22 | 2019-04-12 | 冯涛 | A kind of skin-care gel and preparation method thereof |
| CN115054554A (en) * | 2022-07-20 | 2022-09-16 | 三明学院 | Hispid fig washing-free hand sanitizer and preparation method thereof |
| CN115089629A (en) * | 2022-07-20 | 2022-09-23 | 三明学院 | Freeze-dried hispid fig fruit flavone extracting solution and preparation method thereof |
| CN115089629B (en) * | 2022-07-20 | 2023-09-12 | 三明学院 | Ficus simplicissima lour freeze-dried fruit flavone extracting solution and preparation method thereof |
| CN115054554B (en) * | 2022-07-20 | 2024-03-22 | 三明学院 | Five-finger wild peach no-wash hand cleanser and preparation method thereof |
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