CN115053862A - Method for removing endotoxin from eggs - Google Patents
Method for removing endotoxin from eggs Download PDFInfo
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- CN115053862A CN115053862A CN202210874112.4A CN202210874112A CN115053862A CN 115053862 A CN115053862 A CN 115053862A CN 202210874112 A CN202210874112 A CN 202210874112A CN 115053862 A CN115053862 A CN 115053862A
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- 235000013601 eggs Nutrition 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 23
- 239000002158 endotoxin Substances 0.000 title claims description 12
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- 239000002636 mycotoxin Substances 0.000 claims abstract description 20
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- 108010029541 Laccase Proteins 0.000 claims description 8
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 8
- 239000004472 Lysine Substances 0.000 claims description 8
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- 150000003839 salts Chemical class 0.000 claims description 8
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 claims description 8
- 235000019764 Soybean Meal Nutrition 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 239000004455 soybean meal Substances 0.000 claims description 5
- 241000208688 Eucommia Species 0.000 claims description 4
- 241000208689 Eucommia ulmoides Species 0.000 claims description 4
- 239000003651 drinking water Substances 0.000 claims description 4
- 235000020188 drinking water Nutrition 0.000 claims description 4
- 239000002384 drinking water standard Substances 0.000 claims description 4
- 230000002708 enhancing effect Effects 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
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- 238000001035 drying Methods 0.000 claims 1
- 229930195730 Aflatoxin Natural products 0.000 abstract description 11
- 239000005409 aflatoxin Substances 0.000 abstract description 11
- XWIYFDMXXLINPU-UHFFFAOYSA-N Aflatoxin G Chemical compound O=C1OCCC2=C1C(=O)OC1=C2C(OC)=CC2=C1C1C=COC1O2 XWIYFDMXXLINPU-UHFFFAOYSA-N 0.000 abstract description 10
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- LINOMUASTDIRTM-UHFFFAOYSA-N vomitoxin hydrate Natural products OCC12C(O)C(=O)C(C)=CC1OC1C(O)CC2(C)C11CO1 LINOMUASTDIRTM-UHFFFAOYSA-N 0.000 abstract description 10
- MBMQEIFVQACCCH-QBODLPLBSA-N zearalenone Chemical compound O=C1O[C@@H](C)CCCC(=O)CCC\C=C\C2=CC(O)=CC(O)=C21 MBMQEIFVQACCCH-QBODLPLBSA-N 0.000 abstract description 10
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- HJTAZXHBEBIQQX-UHFFFAOYSA-N 1,5-bis(chloromethyl)naphthalene Chemical compound C1=CC=C2C(CCl)=CC=CC2=C1CCl HJTAZXHBEBIQQX-UHFFFAOYSA-N 0.000 description 1
- 101100449517 Arabidopsis thaliana GRH1 gene Proteins 0.000 description 1
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- OQIQSTLJSLGHID-WNWIJWBNSA-N aflatoxin B1 Chemical compound C=1([C@@H]2C=CO[C@@H]2OC=1C=C(C1=2)OC)C=2OC(=O)C2=C1CCC2=O OQIQSTLJSLGHID-WNWIJWBNSA-N 0.000 description 1
- 229930020125 aflatoxin-B1 Natural products 0.000 description 1
- GOLCXWYRSKYTSP-UHFFFAOYSA-N arsenic trioxide Inorganic materials O1[As]2O[As]1O2 GOLCXWYRSKYTSP-UHFFFAOYSA-N 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 231100000504 carcinogenesis Toxicity 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/22—Compounds of alkali metals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
Abstract
The invention relates to the field of laying hen breeding, in particular to a method for removing mycotoxin in eggs. The invention aims to solve the technical problem that the toxin such as aflatoxin, vomitoxin, zearalenone and the like in the egg is greatly reduced by feeding additives to the laying hens and improving the breeding environment of the laying hens. The invention has the beneficial effects that: aiming at the laying hens, the problems that the mould and mycotoxin in the laying hens are reduced in the feeding process and the laying process are comprehensively solved from various angles of environment, edible feed, scientific cultivation, illumination, water quality, daily disease prevention, special technology and the like, the immunity of the laying hens is improved, and the harm of the mycotoxin can be prevented, so that the mycotoxin in eggs is reduced, and the egg laying efficiency of the laying hens is improved.
Description
Technical Field
The invention relates to the field of laying hen breeding, in particular to a method for removing mycotoxin in eggs.
Background
Mycotoxins are large enemies of food industry, excessive aflatoxins are more toxic than arsenic trioxide, more than 400 mycotoxins have serious harm to human health, and long-term consumption of mycotoxins can cause carcinogenesis, liver diseases, immunosuppressive diseases, intestinal diseases and the like.
Compared with other foods such as milk and the like, the mycotoxin in the eggs does not attract high attention of the industry, and the mycotoxin in the eggs does not have national standards, so that the mycotoxin in the eggs is regarded as a food which is consumed by domestic families every day, and the excessive mycotoxin in the eggs is paid attention.
Because of the uncertainty in the laying hen breeding environment, feed, water sources, waterlines, troughs and the like can have mycotoxin, and residue can be formed in eggs due to long-term accumulation.
The quality of eggs of the eggs on the market is uneven at present, and the eggs laid by the laying hens also contain a large amount of residual toxins such as aflatoxin, vomitoxin, zearalenone and the like, more or less; the aflatoxin is a compound with similar chemical structure, is a derivative of dihydrofurocoumarin, and is a mycotoxin with the largest mycotoxicity and extremely outstanding harm to human health; vomitoxin has strong toxicity to both human and animals, and can cause emesis, diarrhea, skin irritation, food refusal, nerve disorder, abortion, and stillbirth of fetus; zearalenone causes poisoning symptoms of central nervous system such as nausea, chill, headache, mental depression and ataxia to human body.
Disclosure of Invention
The invention aims to solve the technical problem that a method for removing aflatoxin, vomitoxin, zearalenone and other toxins in eggs greatly reduces aflatoxin, vomitoxin, zearalenone and other toxins in eggs laid by laying hens by feeding additives to the laying hens and improving the laying hen breeding environment.
In order to solve the above problems, the present invention provides a method for removing endotoxin from eggs, comprising the steps of: a. optimizing the culture environment: selecting a place with excellent ecological environment for feeding, wherein the periphery of a stocking ground has no large pollution source, the slope of the mountain land is preferably 3-5 degrees, the maximum slope is not more than 25 degrees, and the requirements of good illumination, greenness, rich water source and convenient water taking are met, and the water quality reaches the drinking water standard of people; b. screening chicks: laying hens of 0-5 weeks old are chicks; the temperature of a hen house of 1 week old is 31-33 ℃, the humidity in the hen house is 50-60%, the chicks need to be irradiated for 10-12h after 5 weeks, and the hens are adapted to feed the chicks before 5 weeks; c. breeding the laying hens: after 6 weeks, the laying hens are kept in a constant-temperature henhouse, and after 6 weeks, the laying hens are adapted to egg laying feed; d. daily disease prevention: reducing the breeding density, frequently changing drinking water and timely sterilizing, ventilating air in a chicken house, deeply burying or burning dead chickens to cut off infection sources, establishing an intestinal tract prevention barrier of the laying hens, protecting the livers of the laying hens, lightening the liver burden and enhancing the toxin expelling capability, and using the feed additive to strengthen the direct mycotoxin removing capability.
Further, the feed additive for the laying hens comprises the following components in parts by weight: 5-10 parts of pine needle, 0.5-2 parts of lysine, 2-5 parts of glucose, 3-5 parts of puffed soybean meal, 5-8 parts of stone powder for feed, 3-5 parts of complex enzyme for poultry, 5-8 parts of dried orange peel, 1-3 parts of salt, 20-50 parts of oriental wormwood, 10-25 parts of eucommia ulmoides extract and 20-30 parts of laccase;
preferably, the pine needles are roasted after fresh pine needle branches are defoliated and cut, the roasting temperature is controlled to be 90 ℃, and when the water content of the raw materials is reduced to 8%, the raw materials are ground into powder;
preferably, the dried orange peel is dried orange peel grinding powder with the humidity of below 15%;
preferably, the stone powder for the feed is medical stone powder.
The preparation method of the laying hen feed additive comprises the following steps:
s1, 5-10 parts of pine needle, 0.5-2 parts of lysine, 2-5 parts of glucose, 3-5 parts of puffed soybean meal, 5-8 parts of stone powder for feed, 3-5 parts of complex enzyme for poultry, 5-8 parts of dried orange peel, 1-3 parts of salt, 20-50 parts of oriental wormwood, 10-25 parts of eucommia bark extract and 20-30 parts of laccase, mixing, adding into a pulverizer, pulverizing, filtering and sieving with a 100-mesh sieve; s2, putting the mixture into a granulator for granulation at the temperature of 90 ℃; s3, placing in a dry place for later use.
The invention has the beneficial effects that: aiming at the laying hens, the aims at comprehensively solving the problems of reduction of toxic substances in bodies of the laying hens in the feeding process and the laying process and increase of immunity of the laying hens from various angles of environment, feed eating, scientific cultivation, illumination, water quality, daily disease prevention, special technology and the like, so that mycotoxin in eggs is reduced, and the egg laying efficiency of the laying hens is improved.
Detailed Description
The present invention will be further illustrated with reference to the following examples, but the present invention is not limited thereto, and the preparation methods in the examples are all conventional stirring methods, and will not be described in detail.
Example 1
The invention provides a method for removing endotoxin from eggs, which comprises the following steps: a. optimizing the culture environment: selecting a place with excellent ecological environment for breeding. The method has the advantages that no large pollution source exists around the cultivation land, the slope of the mountain land is preferably 3-5 degrees, the maximum slope is not more than 25 degrees, the illumination is good, the shade is greenish, the water source is rich, the water taking is convenient, and the water quality reaches the drinking water standard of people; b. screening chicks: laying hens of 0-5 weeks old are chicks; the temperature of a hen house of 1 week old is 31-33 ℃, the humidity in the hen house is 50-60%, the chicks need to be irradiated for 10-12h after 5 weeks, and the hens are adapted to feed the chicks before 5 weeks; c. breeding the laying hens: after 6 weeks, the laying hens are kept in a constant-temperature henhouse, and after 6 weeks, the laying hens are adapted to egg laying feed; d. daily disease prevention: reducing the breeding density, changing drinking water frequently and disinfecting in time, circulating air in a chicken house, deeply burying or burning dead chickens to cut off the infection source, protecting the livers of the laying hens, lightening the liver burden and enhancing the detoxification capability, and strengthening the direct removal capability of mycotoxin by using a feed additive.
The feed additive for the laying hens comprises the following components in parts by weight: 5 parts of pine needle, 0.5 part of lysine, 2 parts of glucose, 3 parts of puffed soybean meal, 5 parts of stone powder for feed, 3 parts of complex enzyme for poultry, 5 parts of dried orange peel, 1 part of salt, 20 parts of oriental wormwood, 10 parts of eucommia ulmoides extract and 20 parts of laccase;
preferably, the pine needles are roasted after fresh pine needle branches are defoliated and cut, the roasting temperature is controlled to be 90 ℃, and when the water content of the raw materials is reduced to 8%, the raw materials are ground into powder;
preferably, the dried orange peel is dried orange peel grinding powder with the humidity of below 15%;
preferably, the stone powder for the feed is medical stone powder.
The preparation method of the laying hen feed additive comprises the following steps:
s1, 5 parts of pine needle, 0.5 part of lysine, 2 parts of glucose, 3 parts of puffed soybean powder, 5 parts of stone powder for feed, 3 parts of complex enzyme for poultry, 5 parts of dried orange peel, 1 part of salt, 20 parts of oriental wormwood, 10 parts of eucommia bark extract and 20 parts of laccase, mixing, adding into a grinder for grinding, and filtering by a 100-mesh sieve; s2, putting the mixture into a granulator for granulation at the temperature of 90 ℃; s3, placing in a dry place for later use.
Example 2
The invention provides a method for removing endotoxin from eggs, which comprises the following steps: a. optimizing the culture environment: selecting a place with excellent ecological environment for breeding. The method has the advantages that no large pollution source exists around the cultivation land, the slope of the mountain land is preferably 3-5 degrees, the maximum slope is not more than 25 degrees, the illumination is good, the shade is greenish, the water source is rich, the water taking is convenient, and the water quality reaches the drinking water standard of people; b. screening chicks: laying hens of 0-5 weeks old are chicks; the temperature of a hen house of 1 week old is 31-33 ℃, the humidity in the hen house is 50-60%, the chicks need to be irradiated for 10-12h after 5 weeks, and the hens are adapted to feed the chicks before 5 weeks; c. breeding the laying hens: after 6 weeks, the laying hens are kept in a constant-temperature henhouse, and after 6 weeks, the laying hens are matched with feed in a growing period; d. daily disease prevention: reducing the breeding density, frequently changing drinking water and timely sterilizing, ventilating air in a chicken house, deeply burying or burning dead chickens to cut off infection sources to form an intestinal tract prevention barrier, protecting the livers of the laying hens, lightening the liver burden and enhancing the toxin expelling capability, and using the feed additive to strengthen the direct mycotoxin removing capability.
Further, the feed additive for the laying hens comprises the following components in parts by weight: 8 parts of pine needle, 1 part of lysine, 3 parts of glucose, 5 parts of puffed soybean meal, 7 parts of stone powder for feed, 5 parts of complex enzyme for poultry, 6 parts of dried orange peel, 2 parts of salt, 35 parts of oriental wormwood, 20 parts of eucommia ulmoides extract and 28 parts of laccase;
preferably, the pine needles are roasted after fresh pine needle branches are defoliated and cut, the roasting temperature is controlled to be 90 ℃, and when the water content of the raw materials is reduced to 8%, the raw materials are ground into powder;
preferably, the dried orange peel is dried orange peel grinding powder with the humidity of below 15%;
preferably, the stone powder for the feed is medical stone powder.
The preparation method of the laying hen feed additive comprises the following steps:
s1, 8 parts of pine needle, 1 part of lysine, 3 parts of glucose, 5 parts of puffed soybean powder, 7 parts of stone powder for feed, 5 parts of complex enzyme for poultry, 6 parts of dried orange peel, 2 parts of salt, 35 parts of oriental wormwood, 20 parts of eucommia bark extract and 28 parts of laccase, mixing, adding into a grinder for grinding, and filtering by a 100-mesh sieve; s2, putting the mixture into a granulator for granulation at the temperature of 90 ℃; s3, placing in a dry place for later use.
Experimental data and results:
1. experiment design:
laying hens of 120 days old are selected and randomly divided into 3 groups of 200 laying hens.
2. Experimental feeding:
the experimental components are A, B, C groups, group A is a common feed which is not added with the additive of the invention and is fed as an experimental reference, group B is the formula used in example 1, group C is the formula used in example 2, the feeding period is 60 days, and three eggs are respectively taken from each group after 60 days and are mixed to be used as a sample.
3. And (3) testing experimental indexes:
aflatoxin B1AFB1 (ppb), vomitoxin don (ppb), zearalenone (ppb) were detected using a fluorescence quantitative detector and data analysis was performed.
4. Statistical analysis
The contents of aflatoxin B1AFB1 (ppb), vomitoxin DON (ppb) and zearalenone (ppb) are detected by using a fluorescence quantitative detector, and are shown in the following table 1:
| group of | Group A | Group B | Group C |
| AFB1 (Aspergillus flavus) content/ppb | 0.6 | 0.2 | 0.16 |
| Vomitoxin DON (ppb) | 160 | 70 | 63 |
| Zearalenone (ppb) | 12 | 7 | 6 |
(Table 1)
As can be seen from the table (table 1), the egg has obviously reduced aflatoxin content by using the additive and the common feed prepared according to the formula in the invention, wherein the group B has 33% reduced aflatoxin content, 43% reduced vomitoxin DON (ppb), 58% reduced zearalenone (ppb), 26% reduced group C has a reduced aflatoxin content, 39% reduced vomitoxin DON (ppb) content, and 50% reduced zearalenone (ppb) content.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (7)
1. A method for removing endotoxin from eggs, comprising the steps of:
a. optimizing the culture environment: selecting a place with excellent and suitable ecological environment for feeding, wherein the surrounding of a stocking place has no large pollution source, the slope of the mountain land is preferably 3-5 degrees, the maximum slope is not more than 25 degrees, the illumination is good, the shade is greenish, the water source is rich, the water taking is convenient, and the water quality reaches the drinking water standard of people;
b. screening chicks: laying hens of 0-5 weeks old are chicks; the temperature of a hen house of 1 week old is 31-33 ℃, the humidity in the hen house is 50-60%, the chicks need to be irradiated for 10-12h after 5 weeks, and the hens are adapted to feed the chicks before 5 weeks;
c. breeding the laying hens: after 6 weeks, the laying hens are kept in a constant-temperature henhouse, and after 6 weeks, the laying hens are matched with feed in a growing period;
d. daily disease prevention: the method has the advantages that the feeding density is reduced, drinking water is changed frequently and disinfected timely, the air in the chicken house is circulated, the infection source of the dead chicken needs to be cut off by deep burying or burning, a mycotoxin intestinal tract prevention barrier is established, the liver of the laying hen is protected, the liver burden is relieved, and the toxin expelling capability is enhanced;
e. the feed additive is used for enhancing the direct removal capacity of mycotoxin.
2. The method for removing the endotoxin in the eggs is characterized in that the feed additive for the laying hens consists of the following components in parts by weight: 5-10 parts of pine needle, 0.5-2 parts of lysine, 2-5 parts of glucose, 3-5 parts of puffed soybean powder, 5-8 parts of stone powder for feed, 3-5 parts of complex enzyme for poultry, 5-8 parts of dried orange peel, 1-3 parts of salt, 20-50 parts of oriental wormwood, 10-25 parts of eucommia ulmoides extract and 20-30 parts of laccase.
3. The method for removing endotoxin from eggs, as claimed in claim 2, wherein the pine needles are freshly cut and dried after being defoliated, the drying temperature is controlled at 90 ℃, and when the moisture content of the raw materials is reduced to 8%, the raw materials are ground into powder.
4. The method for removing endotoxin from eggs according to claim 2, wherein the dried orange peel is ground dried orange peel having a humidity of 15% or less.
5. The method for removing endotoxin from eggs as claimed in claim 2, wherein said stone powder for feed is maifanite stone powder.
6. The method for removing endotoxin from eggs as claimed in claim 2, wherein said stone powder for feed is maifanite stone powder.
7. The method for removing endotoxin from eggs, as claimed in claim 2, wherein the preparation method of the laying hen feed additive comprises the following steps:
s1, 5-10 parts of pine needle, 0.5-2 parts of lysine, 2-5 parts of glucose, 3-5 parts of puffed soybean meal, 5-8 parts of stone powder for feed, 3-5 parts of complex enzyme for poultry, 5-8 parts of dried orange peel, 1-3 parts of salt, 20-50 parts of oriental wormwood, 10-25 parts of eucommia bark extract and 20-30 parts of laccase, mixing, adding into a pulverizer, pulverizing, filtering and sieving with a 100-mesh sieve;
s2, putting the mixture into a granulator for granulation at the temperature of 90 ℃;
s3, placing in a dry place for later use.
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