CN115032151B - Efficient multifunctional enzyme-labeling instrument and use method thereof - Google Patents
Efficient multifunctional enzyme-labeling instrument and use method thereof Download PDFInfo
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- CN115032151B CN115032151B CN202210655693.2A CN202210655693A CN115032151B CN 115032151 B CN115032151 B CN 115032151B CN 202210655693 A CN202210655693 A CN 202210655693A CN 115032151 B CN115032151 B CN 115032151B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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Abstract
The invention discloses a high-efficiency multifunctional microplate reader and a use method thereof, and provides a scheme which comprises the microplate reader and a control panel arranged in the microplate reader, wherein a detection bin and a feed inlet which are communicated are arranged in the microplate reader, the inner wall of the bottom of the detection bin is connected with a supporting plate in a sliding manner, a liquid containing vessel is arranged at the top of the supporting plate, a liquid dropping component for injecting a sample to be detected into a liquid storage tank of the liquid containing vessel is arranged at the top of the microplate reader, and a sealing component for sealing the feed inlet is arranged in the feed inlet.
Description
Technical Field
The invention relates to the technical field of microplate readers, in particular to a high-efficiency multifunctional microplate reader and a using method thereof.
Background
The microplate reader is a phase-change photoelectric colorimeter or spectrophotometer in fact, and the basic working principle of the microplate reader is basically the same as that of a main structure and the photoelectric colorimeter. The light wave emitted by the light source lamp is changed into a beam of monochromatic light through the optical filter or the monochromator, and enters the sample to be measured in the plastic micropore pole. One part of the monochromatic light is absorbed by the specimen, the other part of the monochromatic light penetrates through the specimen and irradiates on a photoelectric detector, the photoelectric detector converts the light signals with different strengths of the specimen to be detected into corresponding electric signals, the electric signals are sent into a microprocessor for data processing and calculation after signal processing such as prepositive amplification, logarithmic amplification, analog-to-digital conversion and the like, and finally, the result is displayed by a display and a printer.
Among the prior art, need the staff to await measuring through the sampling tube and instil into the test household utensils using the ELIASA, put into the ELIASA through the tray with the test household utensils again and detect, because the sample that awaits measuring that instils, and still need carry the test household utensils to ELIASA department, consequently seriously influence detection efficiency, and put into the ELIASA in-process through the tray with the test household utensils, because the rupture phenomenon appears in too big easy messenger's tray of test household utensils and the sample weight that awaits measuring, cause the ELIASA, in addition the control panel of ELIASA exposes for a long time, not only make ELIASA occupation space increase still make control panel impaired in the external world easily, and because the ELIASA is placed in the external world, external bacterium, during the dust gets into the ELIASA, influence subsequent detection.
Disclosure of Invention
The invention aims to solve the problems that in the prior art, a sample to be detected needs to be dropped manually, the dropping of the sample to be detected and the detection of an microplate reader are respectively carried out on different devices, the detection efficiency is low, a tray is easy to break, a control panel is damaged outside, outside bacteria and dust are easy to enter the microplate reader, and the occupied space of the control panel is large, and provides a high-efficiency multifunctional microplate reader with a compact structure and a use method thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
a high-efficiency multifunctional microplate reader comprises a microplate reader and a control panel arranged in the microplate reader, wherein a detection bin and a feed inlet which are communicated are arranged in the microplate reader, the inner wall of the bottom of the detection bin is connected with a supporting plate in a sliding manner, and a liquid containing vessel is arranged at the top of the supporting plate;
the top of the microplate reader is provided with a liquid dropping component for injecting a liquid storage tank of a liquid containing vessel with a sample to be detected;
a sealing assembly for sealing the feed inlet is arranged in the feed inlet, and the liquid dropping assembly can control the sealing assembly;
an unfolding component used for unfolding the control panel is arranged in the microplate reader;
a supporting component for supporting the supporting plate is arranged in the detection bin;
the detection bin is internally provided with a driving assembly for driving the supporting plate to move, and the driving assembly can respectively drive the unfolding assembly and the supporting assembly.
Preferably, the dropping liquid subassembly includes two backup pads of fixed connection at the ELIASA top, two rotate between the backup pad and be connected with the pivot, the outer wall fixed cover of pivot is equipped with U type frame, the outer wall cover of pivot is equipped with two first torsional springs, two the one end that first torsional spring is close to each other all with U type frame fixed connection, the other end and the backup pad fixed connection of first torsional spring, one side fixedly connected with sample storehouse of ELIASA is kept away from to U type frame, sliding connection has the piston rod in the sample storehouse, the bottom in sample storehouse is equipped with a plurality of sampling tubes that are linked together, fixedly connected with fixed plate in the U type frame, the bottom fixedly connected with dog of U type frame, the top sliding connection of ELIASA has the guard box that is used for carrying out the protection to a plurality of sampling tubes, and the guard box cooperatees with the dog and can play the braking action to U type frame.
Preferably, the seal assembly is including setting up the spout at feed inlet top inner wall, sliding connection has the closing plate in the spout, a plurality of slide bars of top fixedly connected with of closing plate, and the top of slide bar extends to the top of ELIASA and cooperatees with the fixed plate, the outer wall cover of slide bar is equipped with the extension spring with spout top inner wall fixed connection, and the bottom of extension spring and the top fixed connection of closing plate.
Preferably, the control assembly comprises a rotating groove arranged at the top of the microplate reader, a rotating shaft is connected in the rotating groove, the control panel is fixedly sleeved on the outer wall of the rotating shaft, two second torsion springs are sleeved on the outer wall of the rotating shaft, one ends, close to each other, of the second torsion springs are fixedly connected with the control panel, the other ends of the second torsion springs are fixedly connected with the inner wall of one side of the rotating groove, and a clamping groove is formed in one side of the control panel.
Preferably, the supporting component comprises a sliding groove arranged on the inner wall of the bottom of the detection bin, a supporting rod is connected in the sliding groove in a sliding mode, a first spring is fixedly connected to one end, away from the feeding hole, of the supporting rod, the other end of the first spring is fixedly connected with the inner wall of one side of the sliding groove, and a protruding block located in the feeding hole is fixedly connected to the top of the supporting rod.
Preferably, the driving assembly comprises a transmission groove arranged on the inner wall of the bottom of the detection bin, two synchronizing wheels are rotationally connected in the transmission groove and connected through synchronous belt transmission, a driving motor is arranged in the microplate reader, an output shaft of the driving motor is fixedly connected with a rotating shaft of one of the synchronizing wheels, a connecting block is fixedly connected to the top of each synchronous belt, and the top of each connecting block is fixedly connected with the bottom of the supporting plate.
Preferably, be equipped with the rectangular channel in the ELIASA, sliding connection has the slide in the rectangular channel, the slide is kept away from a plurality of second springs of one side fixedly connected with in the detection storehouse, and the other end of second spring and one side inner wall fixed connection of rectangular channel, the slide is close to one side fixedly connected with fixture block and the push rod in the detection storehouse, and the other end of fixture block extends to and rotates the inslot and cooperate with the draw-in groove, the other end of push rod extends to in the detection storehouse.
Preferably, one side fixedly connected with blotter that U type frame is close to the ELIASA, and the opposite side of blotter touches with the ELIASA, and when the U type frame received under the power of holding of first torsion spring clockwise turning, can play the cushioning effect to U type frame through the blotter, avoided U type frame and ELIASA rigid collision to appear and cause sample storehouse and sampling tube impaired.
Preferably, one side inner wall of U type frame rotates and is connected with the screw rod that is located the piston rod top, the opposite side inner wall fixedly connected with of U type frame rotates the motor, the output shaft of rotation motor and the other end fixed connection of screw rod, the outer wall thread cover of screw rod is equipped with the nut piece, the bottom of nut piece is rotated and is connected with the dwang, and the bottom of dwang rotates with the top of piston rod and is connected, starts to rotate motor drive screw and rotates, and the screw rod drives the nut piece and removes, and the removal of nut piece can drive the dwang and rotate, and then the dwang can promote the piston rod and move down, accomplishes the sample injection operation that awaits measuring, need not the manual work and press the piston rod alright accomplish the sample injection operation that awaits measuring, just can pour into equivalent sample that awaits measuring in the reservoir in the flourishing liquid dish through starting to rotate the motor.
The use method of the high-efficiency multifunctional microplate reader comprises the following steps:
s1, the protection box is pushed to the left side and is separated from the stop block, and due to the fact that the first torsion spring is always in a force storage state, after the stop block loses the braking of the protection box, the U-shaped frame rotates clockwise under the action of the first torsion spring, the buffer cushion can be in contact with one side of the microplate reader, and the sampling tube is in a vertical state;
s2, a driving motor is started to drive synchronous wheels to rotate, the two synchronous wheels are connected through synchronous belt transmission, then the synchronous belts drive a connecting block to start to move a certain distance to the left, the connecting block drives a supporting plate to move a certain distance to the left, the supporting plate pushes a push rod and a sliding plate to move the left and extrudes a second spring, the sliding plate drives a clamping block to be separated from a clamping groove, the second torsion spring is always in force storage rotation, and after the control panel loses the braking of the clamping block, the control panel starts to rotate under the action of the second torsion spring, so that the control panel is separated from the rotating groove, and the control of workers is facilitated;
s3, after the U-shaped frame rotates clockwise, the fixed plate loses the pushing of the sliding rod, the sliding rod drives the sealing plate to enter the sliding groove from the feeding hole under the pulling force of the tension spring, so that the feeding hole is opened, and the supporting plate and the liquid containing vessel can conveniently enter and exit the detection bin;
s4, the supporting plate and the liquid containing vessel are driven by the connecting block to move towards the right side, when the supporting plate enters the feeding hole, the supporting plate is contacted with the lug, the supporting plate drives the supporting rod to slide towards the right side through the lug along with the continuous movement of the supporting plate, the first spring begins to stretch, the supporting plate can drive the liquid containing vessel to move towards the outer side of the enzyme labeling instrument along with the movement of the liquid containing vessel and the supporting rod driven by the supporting plate, the supporting rod can lift the supporting plate and the supporting plate, and the supporting plate is prevented from being broken by the gravity of the liquid containing vessel and the gravity of a sample in the liquid containing vessel;
s5, when the liquid containing vessel is moved out of the feed inlet, the piston rod is pressed downwards, the piston rod can inject samples to be detected in the sampling bin into the storage grooves in the liquid containing vessel through the sampling tubes respectively, then the connecting block drives the liquid containing vessel to move for a certain distance through the supporting plate and then stops, then the samples to be detected are injected into the storage grooves in the upper row and the lower row of the liquid containing vessel by pressing again, the storage grooves in the liquid containing vessel can be filled with the samples to be detected through reciprocating operation, and then the injection and the detection of the samples to be detected can be carried out on the same equipment.
Compared with the prior art, the invention provides a high-efficiency multifunctional microplate reader, which has the following beneficial effects:
1. this high-efficient multi-functional ELIASA's feed inlet's top inner wall is equipped with the spout, sliding connection has the closing plate in the spout, a plurality of slide bars of top fixedly connected with of closing plate, and the top of slide bar extends to the top of ELIASA, the outer wall cover of slide bar be equipped with spout top inner wall fixed connection's extension spring, and the bottom of extension spring and the top fixed connection of closing plate, after U type frame clockwise rotation, the slide bar loses the braking of U type frame, the closing plate and then can be at the pulling force of extension spring rebound down, and then can open the feed inlet, otherwise can seal the feed inlet and avoid during external bacterium, dust get into the detection storehouse, the influence detects.
2. This rotation inslot rotation of high-efficient multi-functional ELIASA is connected with the axis of rotation, the fixed cover of outer wall of axis of rotation is equipped with control panel, the outer wall cover of axis of rotation is equipped with two second torsional springs, two the one end that the second torsional spring is close to each other all with control panel fixed connection, the other end of second torsional spring and one side inner wall fixed connection who rotates the groove, one side of control panel is equipped with the draw-in groove with fixture block matched with, when the layer board removes and promotes push rod and slide to the left side, the slide drives the fixture block and breaks away from the draw-in groove, and then control panel rotates under the power of holding of second torsional spring, and control panel goes out from rotating the groove, places not only can accomodate control panel to rotating the groove in and avoid control panel impaired can also reduce the space that control panel occupy.
3. This high-efficient multi-functional ELIASA's sliding tray sliding connection has the die-pin, the first spring of one end fixedly connected with of feed inlet is kept away from to the die-pin, and the other end of first spring and one side inner wall fixed connection of sliding tray, the top fixedly connected with of die-pin is located the intraoral lug of feed inlet, and when layer board and flourishing liquid ware promoted the die-pin through the lug and move to the outside, the die-pin can play the supporting role to the layer board, avoids flourishing liquid ware to fold the layer board after greatly getting too much to be measured sample.
4. This two backup pads of one side fixedly connected with of ELIASA of high-efficient multi-functional ELIASA, two rotate between the backup pad and be connected with the pivot, the outer wall fixed cover of pivot is equipped with U type frame, one side fixedly connected with sample storehouse that the ELIASA was kept away from to U type frame, the bottom in sample storehouse is equipped with a plurality of sampling tubes that are linked together, and U type frame begins clockwise rotation under the effect of holding power of first torsional spring, and then can make the sampling tube be in the vertical state, is convenient for pour into the sample that awaits measuring into to liquid containing dish on the ELIASA, can go on the injection of the sample that awaits measuring and detect at same equipment, avoids the transport to liquid containing dish to improve detection efficiency.
The sampling tube is in a vertical state, so that a sample to be detected can be injected later, the feeding hole can be automatically opened, the opening and the closing of the feeding hole are completed, the brake on the control panel can be released through the movement of the supporting plate, the containing and the unfolding of the control panel can be controlled, and the supporting rod and the lug are matched to play a supporting role on the supporting plate when the supporting plate moves outwards, so that the supporting plate is prevented from being broken.
Drawings
FIG. 1 is a front sectional view of a high-efficiency multifunctional microplate reader provided by the invention;
FIG. 2 is an enlarged view of FIG. 1 at A;
FIG. 3 is a sectional view taken along line B-B of FIG. 1;
FIG. 4 is a cross-sectional view taken at C-C of FIG. 3;
FIG. 5 is a three-dimensional cross-sectional view of a microplate reader of the high-efficiency multifunctional microplate reader provided by the invention;
FIG. 6 is a first perspective three-dimensional view of a dropping liquid assembly of the high-efficiency multifunctional microplate reader provided by the invention;
FIG. 7 is a second perspective three-dimensional view of a dropping liquid assembly of the high-efficiency multifunctional microplate reader provided by the invention;
FIG. 8 is a three-dimensional view of the sealing assembly of the high-efficiency multifunctional microplate reader provided by the invention;
FIG. 9 is a three-dimensional view of a dropping liquid assembly of the multifunctional microplate reader according to the second embodiment of the invention.
In the figure: 1. an enzyme-labeling instrument; 2. a detection bin; 3. a feed inlet; 4. a support plate; 5. a liquid container; 6. a protection box; 7. a support plate; 8. a rotating shaft; 9. a U-shaped frame; 10. a first torsion spring; 11. sampling a bin; 12. a sampling tube; 13. a stopper; 14. a chute; 15. a closing plate; 16. a slide bar; 17. a tension spring; 18. a fixing plate; 19. a transmission groove; 20. a synchronizing wheel; 21. a synchronous belt; 22. a drive motor; 23. connecting blocks; 24. a sliding groove; 25. a first spring; 26. a support rod; 27. a bump; 28. a rotating groove; 29. a control panel; 30. a rotating shaft; 31. a second torsion spring; 32. a card slot; 33. a rectangular groove; 34. a slide plate; 35. a second spring; 36. a clamping block; 37. a push rod; 38. a cushion pad; 39. rotating the motor; 40. a screw; 41. a nut block; 42. rotating the rod; 43. a piston rod.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
Example 1:
referring to fig. 1-8, a high-efficiency multifunctional microplate reader, comprising a microplate reader 1 and a control panel 29 arranged in the microplate reader 1, a detection bin 2 and a feed inlet 3 which are communicated with each other are arranged in the microplate reader 1, the bottom inner wall of the detection bin 2 is connected with a supporting plate 4 in a sliding manner, the top of the supporting plate 4 is provided with a liquid containing vessel 5, the top of the microplate reader 1 is provided with a liquid dropping component for injecting a sample to be detected into a liquid storage tank of the liquid containing vessel 5, the feed inlet 3 is internally provided with a sealing component for sealing the feed inlet 3, the liquid dropping component can control the sealing component, the microplate reader 1 is internally provided with an expansion component for expanding the control panel 29, the detection bin 2 is internally provided with a supporting component for supporting the supporting plate 4, detect to be equipped with in the 2 drive assembly who is used for driving layer board 4 to remove in the storehouse, and drive assembly can drive respectively and expand subassembly and supporting component, be equipped with rectangular channel 33 in the ELIASA 1, sliding connection has slide 34 in the rectangular channel 33, slide 34 keeps away from a plurality of second springs 35 of one side fixedly connected with that detect storehouse 2, and the other end of second spring 35 and one side inner wall fixed connection of rectangular channel 33, slide 34 is close to one side that detects storehouse 2 and passes through bolt fixedly connected with fixture block 36 and push rod 37, and the other end of fixture block 36 extends to and rotates in the groove 28 and cooperate with draw-in groove 32, the other end of push rod 37 extends to in detecting storehouse 2.
According to the invention, the dropping liquid assembly comprises two support plates 7 fixedly connected to the top of an ELIASA 1, a rotating shaft 8 is rotatably connected between the two support plates 7, a U-shaped frame 9 is fixedly sleeved on the outer wall of the rotating shaft 8, two first torsion springs 10 are sleeved on the outer wall of the rotating shaft 8, one ends of the two first torsion springs 10, which are close to each other, are fixedly connected with the U-shaped frame 9, the other end of the first torsion spring 10 is fixedly connected with the support plates 7, a sampling bin 11 is fixedly connected to one side, far away from the ELIASA 1, of the U-shaped frame 9, a piston rod 43 is slidably connected in the sampling bin 11, a plurality of communicated sampling tubes 12 are arranged at the bottom of the sampling bin 11, a fixing plate 18 is fixedly connected in the U-shaped frame 9 through bolts, a stop block 13 is fixedly connected to the bottom of the U-shaped frame 9, a protection box 6 used for protecting the plurality of the sampling tubes 12 is slidably connected to the top of the ELIASA 1, the protection box 6 and the stop block 13 are matched with each other to play a braking role on the U-shaped frame 9, the U-shaped frame 9 starts to rotate under the force accumulation effect of the first torsion spring 10, so that the lower opening tube 12 can be in a vertical state, the ELIASA 1 can conveniently, the sample 5 can be conveniently, the same sample can be injected into a carrying liquid container 5, the same carrying device to be detected, and the carrying device can be detected, and the carrying efficiency of the same carrying device can be improved, and the carrying device can be improved.
According to the invention, the sealing assembly comprises a sliding groove 14 arranged on the inner wall of the top of the feed port 3, a sealing plate 15 is connected in the sliding groove 14 in a sliding manner, a plurality of sliding rods 16 are fixedly connected to the top of the sealing plate 15 through bolts, the top ends of the sliding rods 16 extend to the upper side of the microplate reader 1 and are matched with a fixing plate 18, a tension spring 17 fixedly connected with the inner wall of the top of the sliding groove 14 is sleeved on the outer wall of each sliding rod 16, the bottom end of the tension spring 17 is fixedly connected with the top of the sealing plate 15, after the U-shaped frame 9 rotates clockwise, the sliding rods 16 lose the braking of the U-shaped frame 9, the sealing plate 15 can move upwards under the tension of the tension spring 17, the feed port 3 can be opened, and otherwise, the feed port 3 can be sealed to prevent external bacteria and dust from entering the detection bin 2 to influence detection.
In the invention, the control assembly comprises a rotating groove 28 arranged at the top of the microplate reader 1, a rotating shaft 30 is rotatably connected in the rotating groove 28, a control panel 29 is fixedly sleeved on the outer wall of the rotating shaft 30, two second torsion springs 31 are sleeved on the outer wall of the rotating shaft 30, one ends of the two second torsion springs 31 close to each other are fixedly connected with the control panel 29, the other ends of the second torsion springs 31 are fixedly connected with the inner wall of one side of the rotating groove 28, one side of the control panel 29 is provided with a clamping groove 32, when the supporting plate 4 moves towards the left side and pushes the push rod 37 and the sliding plate 34, the sliding plate 34 drives the clamping block 36 to be separated from the clamping groove 32, the control panel 29 rotates under the force accumulation of the second torsion springs 31, the control panel 29 is rotated out from the rotating groove 28, and the control panel 29 can be placed to be accommodated in the rotating groove 28 to avoid the damage to the control panel 29 and reduce the space occupied by the control panel 29.
In the invention, the supporting component comprises a sliding groove 24 arranged on the inner wall of the bottom of the detection bin 2, a supporting rod 26 is connected in the sliding groove 24 in a sliding manner, one end of the supporting rod 26, which is far away from the feed port 3, is fixedly connected with a first spring 25, the other end of the first spring 25 is fixedly connected with the inner wall of one side of the sliding groove 24, the top of the supporting rod 26 is fixedly connected with a lug 27 positioned in the feed port 3 through a bolt, and when the supporting plate 4 and the liquid containing vessel 5 push the supporting rod 26 to move outwards through the lug 27, the supporting rod 26 can play a supporting role on the supporting plate 4, so that the supporting plate 4 is prevented from being folded after the liquid containing vessel 5 contains too many samples to be detected.
In the invention, the driving assembly comprises a transmission groove 19 arranged on the inner wall of the bottom of the detection bin 2, two synchronizing wheels 20 are rotatably connected in the transmission groove 19, the two synchronizing wheels 20 are in transmission connection through a synchronous belt 21, a driving motor 22 is arranged in the microplate reader 1, an output shaft of the driving motor 22 is fixedly connected with a rotating shaft of one of the synchronizing wheels 20, the top of the synchronous belt 21 is fixedly connected with a connecting block 23 through a bolt, and the top end of the connecting block 23 is fixedly connected with the bottom of the supporting plate 4 through a bolt.
In the invention, one side of the U-shaped frame 9, which is close to the microplate reader 1, is fixedly connected with the buffer pad 38 through the bolt, the other side of the buffer pad 38 is contacted with the microplate reader 1, and when the U-shaped frame 9 rotates clockwise under the action of the stored force of the first torsion spring 10, the buffer pad 38 can play a buffer role on the U-shaped frame 9, so that the sample bin 11 and the sample tube 12 are prevented from being damaged due to the hard collision between the U-shaped frame 9 and the microplate reader 1.
Example 2:
referring to fig. 1-9, a high-efficiency multifunctional microplate reader, comprising a microplate reader 1 and a control panel 29 arranged in the microplate reader 1, a detection bin 2 and a feed inlet 3 which are communicated with each other are arranged in the microplate reader 1, the bottom inner wall of the detection bin 2 is connected with a supporting plate 4 in a sliding manner, the top of the supporting plate 4 is provided with a liquid containing vessel 5, the top of the microplate reader 1 is provided with a liquid dropping component for injecting a sample to be detected into a liquid storage tank of the liquid containing vessel 5, the feed inlet 3 is internally provided with a sealing component for sealing the feed inlet 3, the liquid dropping component can control the sealing component, the microplate reader 1 is internally provided with an expansion component for expanding the control panel 29, the detection bin 2 is internally provided with a supporting component for supporting the supporting plate 4, detect to be equipped with in the 2 drive assembly who is used for driving layer board 4 to remove in the storehouse, and drive assembly can drive respectively and expand subassembly and supporting component, be equipped with rectangular channel 33 in the ELIASA 1, sliding connection has slide 34 in the rectangular channel 33, slide 34 keeps away from a plurality of second springs 35 of one side fixedly connected with that detect storehouse 2, and the other end of second spring 35 and one side inner wall fixed connection of rectangular channel 33, slide 34 is close to one side that detects storehouse 2 and passes through bolt fixedly connected with fixture block 36 and push rod 37, and the other end of fixture block 36 extends to and rotates in the groove 28 and cooperate with draw-in groove 32, the other end of push rod 37 extends to in detecting storehouse 2.
According to the invention, the dropping liquid assembly comprises two supporting plates 7 fixedly connected to the top of the microplate reader 1, a rotating shaft 8 is rotatably connected between the two supporting plates 7, a U-shaped frame 9 is fixedly sleeved on the outer wall of the rotating shaft 8, two first torsion springs 10 are sleeved on the outer wall of the rotating shaft 8, one ends, close to each other, of the two first torsion springs 10 are fixedly connected with the U-shaped frame 9, the other end of each first torsion spring 10 is fixedly connected with the supporting plate 7, a sampling bin 11 is fixedly connected to one side, far away from the microplate reader 1, of the U-shaped frame 9, a piston rod 43 is slidably connected in the sampling bin 11, a plurality of sampling tubes 12 communicated with each other are arranged at the bottom of the sampling bin 11, a fixing plate 18 is fixedly connected in the U-shaped frame 9 through bolts, a stop block 13 is fixedly connected to the bottom of the U-shaped frame 9, a protection box 6 used for protecting the plurality of the sampling tubes 12 is slidably connected to the top of the microplate reader 1, the protection box 6 is matched with the protection box 13 and can play a braking role in the U-shaped frame 9, the role in stopping the U-shaped frame 9 under the force accumulation effect of the first torsion springs 10, so that the lower opening of the protection box 12 is in a vertical state, the microplate reader is convenient for injecting the sample into the same sample container 5, and the detection liquid to be detected in the same container 5, and detection device can be detected, and detection efficiency of the detection container 5 can be improved.
According to the invention, the sealing assembly comprises a sliding groove 14 arranged on the inner wall of the top of the feed port 3, a sealing plate 15 is connected in the sliding groove 14 in a sliding manner, a plurality of sliding rods 16 are fixedly connected to the top of the sealing plate 15 through bolts, the top ends of the sliding rods 16 extend to the upper side of the microplate reader 1 and are matched with a fixing plate 18, a tension spring 17 fixedly connected with the inner wall of the top of the sliding groove 14 is sleeved on the outer wall of each sliding rod 16, the bottom end of the tension spring 17 is fixedly connected with the top of the sealing plate 15, after the U-shaped frame 9 rotates clockwise, the sliding rods 16 lose the braking of the U-shaped frame 9, the sealing plate 15 can move upwards under the tension of the tension spring 17, the feed port 3 can be opened, and otherwise, the feed port 3 can be sealed to prevent external bacteria and dust from entering the detection bin 2 to influence detection.
In the invention, the control assembly comprises a rotating groove 28 arranged at the top of the microplate reader 1, a rotating shaft 30 is rotatably connected in the rotating groove 28, a control panel 29 is fixedly sleeved on the outer wall of the rotating shaft 30, two second torsion springs 31 are sleeved on the outer wall of the rotating shaft 30, one ends of the two second torsion springs 31 close to each other are fixedly connected with the control panel 29, the other ends of the second torsion springs 31 are fixedly connected with the inner wall of one side of the rotating groove 28, one side of the control panel 29 is provided with a clamping groove 32, when the supporting plate 4 moves towards the left side and pushes the push rod 37 and the sliding plate 34, the sliding plate 34 drives the clamping block 36 to be separated from the clamping groove 32, the control panel 29 rotates under the force accumulation of the second torsion springs 31, the control panel 29 is rotated out from the rotating groove 28, and the control panel 29 can be placed to be accommodated in the rotating groove 28 to avoid the damage to the control panel 29 and reduce the space occupied by the control panel 29.
In the invention, the supporting assembly comprises a sliding groove 24 arranged on the inner wall of the bottom of the detection bin 2, a supporting rod 26 is connected in the sliding groove 24 in a sliding mode, one end, far away from the feed port 3, of the supporting rod 26 is fixedly connected with a first spring 25, the other end of the first spring 25 is fixedly connected with the inner wall of one side of the sliding groove 24, the top of the supporting rod 26 is fixedly connected with a lug 27 located in the feed port 3 through a bolt, when the supporting plate 4 and the liquid containing vessel 5 push the supporting rod 26 to move outwards through the lug 27, the supporting rod 26 can support the supporting plate 4, and the supporting plate 4 is prevented from being folded after the liquid containing vessel 5 contains too many samples to be detected.
In the invention, the driving assembly comprises a transmission groove 19 arranged on the inner wall of the bottom of the detection bin 2, two synchronizing wheels 20 are rotatably connected in the transmission groove 19, the two synchronizing wheels 20 are in transmission connection through a synchronous belt 21, a driving motor 22 is arranged in the microplate reader 1, an output shaft of the driving motor 22 is fixedly connected with a rotating shaft of one of the synchronizing wheels 20, the top of the synchronous belt 21 is fixedly connected with a connecting block 23 through a bolt, and the top end of the connecting block 23 is fixedly connected with the bottom of the supporting plate 4 through a bolt.
In the invention, one side of the U-shaped frame 9, which is close to the microplate reader 1, is fixedly connected with the buffer pad 38 through the bolt, the other side of the buffer pad 38 is contacted with the microplate reader 1, and when the U-shaped frame 9 rotates clockwise under the action of the stored force of the first torsion spring 10, the buffer pad 38 can play a buffer role on the U-shaped frame 9, so that the sample bin 11 and the sample tube 12 are prevented from being damaged due to the hard collision between the U-shaped frame 9 and the microplate reader 1.
In the invention, a screw 40 positioned above a piston rod 43 is rotatably connected to the inner wall of one side of a U-shaped frame 9, a rotating motor 39 is fixedly connected to the inner wall of the other side of the U-shaped frame 9 through a bolt, an output shaft of the rotating motor 39 is fixedly connected with the other end of the screw 40 through a coupler, a nut block 41 is sleeved on the outer wall of the screw 40 in a threaded manner, a rotating rod 42 is rotatably connected to the bottom of the nut block 41, the bottom end of the rotating rod 42 is rotatably connected with the top end of the piston rod 43, the rotating motor 39 is started to drive the screw 40 to rotate, the screw 40 drives the nut block 41 to move, the rotating rod 42 can be driven to rotate by the movement of the nut block 41, further, the piston rod 43 can be pushed by the rotating rod 42 to move downwards, the injection operation of a sample to be detected is completed, the injection operation of the sample to be detected can be completed without manually pressing the piston rod 43, and the same amount of the sample to be detected can be injected into a liquid storage tank in a liquid container 5 by starting the rotating motor 39.
A use method of a high-efficiency multifunctional microplate reader comprises the following steps:
s1, the protection box 6 is pushed to the left side, the protection box 6 is separated from the stop block 13 to be touched, and as the first torsion spring 10 is always in a force accumulation state before, after the stop block 13 loses the braking of the protection box 6, the U-shaped frame 9 rotates clockwise under the action of the first torsion spring 10, the buffer pad 38 can be touched with one side of the microplate reader 1, and the sampling tube 12 is in a vertical state;
s2, a driving motor 22 is started to drive the synchronizing wheels 20 to rotate, the two synchronizing wheels 20 are in transmission connection through a synchronous belt 21, then the synchronous belt 21 drives a connecting block 23 to start to move a certain distance to the left side, the connecting block 23 drives a supporting plate 4 to move a certain distance to the left, the supporting plate 4 pushes a push rod 37 and a sliding plate 34 to move left and extrude a second spring 35, and the sliding plate 34 drives a clamping block 36 to be separated from a clamping groove 32;
s3, after the U-shaped frame 9 rotates clockwise, the fixed plate 18 loses the pushing of the slide rod 16, the slide rod 16 drives the sealing plate 15 to enter the chute 14 from the feed port 3 under the pulling force of the tension spring 17, so that the feed port 3 is opened, and the supporting plate 4 and the liquid containing vessel 5 can conveniently enter and exit the detection bin 2;
s4, then the connecting block 23 drives the supporting plate 4 and the liquid containing vessel 5 to move towards the right side, after the supporting plate 4 enters the feeding hole 3, the supporting plate 4 is in contact with the lug 27, along with the continuous movement of the supporting plate 4, the supporting plate 4 drives the supporting rod 26 to slide towards the right side through the lug 27, the first spring 25 begins to stretch, along with the movement of the liquid containing vessel 5 and the supporting rod 26 driven by the supporting plate 4, the supporting plate 4 can drive the liquid containing vessel 5 to move towards the outer side of the microplate reader 1, and the supporting rod 26 can lift the supporting plate 4 and the supporting plate 4, so that the supporting plate 4 is prevented from being broken due to the gravity of the liquid containing vessel 5 and the gravity of a sample in the liquid containing vessel 5;
s5, when the liquid containing vessel 5 is moved out from the feed inlet 3, the piston rod 43 is pressed downwards, the piston rod 43 can inject the sample to be detected in the sampling bin 11 into the storage tank on the liquid containing vessel 5 through the sampling tubes 12 respectively, then the connecting block 23 drives the liquid containing vessel 5 to move for a certain distance through the supporting plate 4 and then stops, then the sample to be detected is injected into the storage tank on the upper row and the lower row of the liquid containing vessel 5 by pressing again, the reciprocating operation can fill the storage tank on the liquid containing vessel 5 with the sample to be detected, and then the injection and the detection of the sample to be detected can be carried out on the same equipment.
However, the operation principle and wiring method of the microplate reader 1, the rotating motor 39, the driving motor 22 and the control panel 29, which are well known to those skilled in the art, are conventional means or common knowledge in the art, and will not be described herein, and those skilled in the art can make any choice according to their needs or convenience.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be able to cover the technical scope of the present invention and the equivalent alternatives or modifications according to the technical solution and the inventive concept of the present invention within the technical scope of the present invention.
Claims (9)
1. A high-efficiency multifunctional microplate reader comprises a microplate reader (1) and a control panel (29) arranged in the microplate reader (1), and is characterized in that a detection bin (2) and a feed inlet (3) which are communicated with each other are arranged in the microplate reader (1), the inner wall of the bottom of the detection bin (2) is connected with a supporting plate (4) in a sliding manner, and the top of the supporting plate (4) is provided with a liquid containing vessel (5);
the top of the microplate reader (1) is provided with a dropping liquid assembly for injecting a sample to be detected into a liquid storage tank of the liquid containing vessel (5);
a sealing assembly for sealing the feed port (3) is arranged in the feed port (3), and the dropping liquid assembly can control the sealing assembly;
an unfolding component used for unfolding the control panel (29) is arranged in the microplate reader (1);
a supporting component for supporting the supporting plate (4) is arranged in the detection bin (2);
a driving assembly for driving the supporting plate (4) to move is arranged in the detection bin (2), and the driving assembly can respectively drive the unfolding assembly and the supporting assembly;
dropping liquid subassembly includes two backup pads (7) of fixed connection at ELIASA (1) top, two rotate between backup pad (7) and be connected with pivot (8), the outer wall fixed cover of pivot (8) is equipped with U type frame (9), the outer wall cover of pivot (8) is equipped with two first torsional springs (10), two the one end that first torsional spring (10) are close to each other all with U type frame (9) fixed connection, the other end and backup pad (7) fixed connection of first torsional spring (10), one side fixedly connected with sample storehouse (11) of ELIASA (1) are kept away from in U type frame (9), sliding connection has piston rod (43) in sample storehouse (11), the bottom in sample storehouse (11) is equipped with a plurality of sampling tube (12) that are linked together, fixedly connected with fixed plate (18) in U type frame (9), the bottom fixedly connected with dog (13) of U type frame (9), the top sliding connection of ELIASA (1) has protecting box (6) that are used for carrying out the protection to a plurality of tubes (12), and plays protecting box (6) and U type frame (9) and braking action to cooperate.
2. The efficient multifunctional microplate reader as claimed in claim 1, wherein the sealing assembly comprises a sliding groove (14) formed in the inner wall of the top of the feed port (3), a sealing plate (15) is connected in the sliding groove (14), a plurality of sliding rods (16) are fixedly connected to the top of the sealing plate (15), the top ends of the sliding rods (16) extend to the upper side of the microplate reader (1) and are matched with a fixing plate (18), a tension spring (17) fixedly connected with the inner wall of the top of the sliding groove (14) is sleeved on the outer wall of each sliding rod (16), and the bottom end of each tension spring (17) is fixedly connected with the top of the sealing plate (15).
3. The high-efficiency multifunctional microplate reader as claimed in claim 1, characterized in that the microplate reader further comprises a control assembly, the control assembly comprises a rotating groove (28) arranged at the top of the microplate reader (1), a rotating shaft (30) is rotationally connected in the rotating groove (28), the control panel (29) is fixedly sleeved on the outer wall of the rotating shaft (30), two second torsion springs (31) are sleeved on the outer wall of the rotating shaft (30), one ends of the second torsion springs (31) close to each other are fixedly connected with the control panel (29), the other ends of the second torsion springs (31) are fixedly connected with the inner wall of one side of the rotating groove (28), and a clamping groove (32) is arranged on one side of the control panel (29).
4. The high-efficiency multifunctional microplate reader as claimed in claim 1, wherein the support assembly comprises a sliding groove (24) arranged on the inner wall of the bottom of the detection bin (2), a support rod (26) is connected in the sliding groove (24), one end of the support rod (26) far away from the feed port (3) is fixedly connected with a first spring (25), the other end of the first spring (25) is fixedly connected with the inner wall of one side of the sliding groove (24), and the top of the support rod (26) is fixedly connected with a bump (27) positioned in the feed port (3).
5. The high-efficiency multifunctional microplate reader as claimed in claim 1, wherein the driving assembly comprises a transmission groove (19) arranged on the inner wall of the bottom of the detection bin (2), two synchronizing wheels (20) are rotationally connected in the transmission groove (19), the two synchronizing wheels (20) are in transmission connection through a synchronizing belt (21), a driving motor (22) is arranged in the microplate reader (1), an output shaft of the driving motor (22) is fixedly connected with a rotating shaft of one synchronizing wheel (20), a connecting block (23) is fixedly connected to the top of the synchronizing belt (21), and the top of the connecting block (23) is fixedly connected with the bottom of the supporting plate (4).
6. The high-efficiency multifunctional microplate reader according to claim 1, characterized in that a rectangular groove (33) is arranged in the microplate reader (1), a sliding plate (34) is connected in the rectangular groove (33) in a sliding manner, one side of the sliding plate (34) far away from the detection bin (2) is fixedly connected with a plurality of second springs (35), the other end of each second spring (35) is fixedly connected with the inner wall of one side of the rectangular groove (33), one side of the sliding plate (34) close to the detection bin (2) is fixedly connected with a clamping block (36) and a push rod (37), the other end of the clamping block (36) extends into the rotating groove (28) and is matched with the clamping groove (32), and the other end of the push rod (37) extends into the detection bin (2).
7. The microplate reader of claim 1, wherein a buffer pad (38) is fixedly connected to one side of the U-shaped frame (9) close to the microplate reader (1), and the other side of the buffer pad (38) is in contact with the microplate reader (1).
8. The high-efficiency multifunctional microplate reader as claimed in claim 1, wherein the inner wall of one side of the U-shaped frame (9) is rotatably connected with a screw rod (40) positioned above the piston rod (43), the inner wall of the other side of the U-shaped frame (9) is fixedly connected with a rotating motor (39), the output shaft of the rotating motor (39) is fixedly connected with the other end of the screw rod (40), the outer wall of the screw rod (40) is sleeved with a nut block (41), the bottom of the nut block (41) is rotatably connected with a rotating rod (42), and the bottom end of the rotating rod (42) is rotatably connected with the top end of the piston rod (43).
9. The use method of the high-efficiency multifunctional microplate reader according to any one of claims 1-8, characterized by comprising the following steps:
s1, a protection box (6) is pushed to the left side, the protection box (6) is separated from a stop block (13) to be touched, and due to the fact that a first torsion spring (10) is always in a force storage state, after the stop block (13) loses the braking of the protection box (6), a U-shaped frame (9) rotates clockwise under the action of the first torsion spring (10), a cushion pad (38) can be touched with one side of a microplate reader (1), and a sampling tube (12) is in a vertical state;
s2, a driving motor (22) is started to drive synchronous wheels (20) to rotate, the two synchronous wheels (20) are in transmission connection through a synchronous belt (21), the synchronous belt (21) drives a connecting block (23) to start to move to the left for a certain distance, the connecting block (23) drives a supporting plate (4) to move to the left for a certain distance, the supporting plate (4) pushes a push rod (37) and a sliding plate (34) to move to the left and extrude a second spring (35), the sliding plate (34) drives a clamping block (36) to be separated from a clamping groove (32), and since the second torsion spring (31) is always in power storage rotation, after the control panel (29) loses the braking of the clamping block (36), the control panel (29) starts to rotate under the action of the second torsion spring (31), and therefore the control panel (29) is separated from a rotating groove (28);
s3, after the U-shaped frame (9) rotates clockwise, the fixed plate (18) loses pushing of the sliding rod (16), and the sliding rod (16) drives the closing plate (15) to enter the sliding groove (14) from the feeding hole (3) under the pulling force of the tension spring (17), so that the feeding hole (3) is opened;
s4, then the connecting block (23) drives the supporting plate (4) and the liquid containing vessel (5) to move towards the right side, after the supporting plate (4) enters the feeding hole (3), the supporting plate (4) is in contact with the lug (27), along with the continuous movement of the supporting plate (4), the supporting plate (4) drives the supporting rod (26) to slide towards the right side through the lug (27), the first spring (25) starts to stretch, along with the movement of the liquid containing vessel (5) and the supporting rod (26) driven by the supporting plate (4), the supporting plate (4) can drive the liquid containing vessel (5) to move towards the outer side of the microplate reader (1), and the supporting rod (26) can lift the supporting plate (4) and the supporting plate (4);
s5, when flourishing liquid dish (5) shifted out from feed inlet (3), piston rod (43) are pressed down, piston rod (43) can pour into the sample to be measured in sample storehouse (11) into the accumulator tank on flourishing liquid dish (5) respectively through a plurality of sampling tubes (12), stop after connecting block (23) drive flourishing liquid dish (5) through layer board (4) and remove certain distance in to detecting storehouse (2), press again and pour into the sample to be measured in the accumulator tank of flourishing liquid dish (5) upper and lower one row after that, reciprocal operation can fill up the accumulator tank on flourishing liquid dish (5) with the sample to be measured.
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| CN202210655693.2A CN115032151B (en) | 2022-06-10 | 2022-06-10 | Efficient multifunctional enzyme-labeling instrument and use method thereof |
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| US6448090B1 (en) * | 1999-07-09 | 2002-09-10 | Orchid Biosciences, Inc. | Fluid delivery system for a microfluidic device using alternating pressure waveforms |
| CN202049160U (en) * | 2011-05-03 | 2011-11-23 | 上海尧浩生物技术有限公司 | COxB (Coxsackievirus B) IgG/IgM (immunoglobulin G/immunoglobulin M) detection reagent kit by enzyme-linked immunosorbent assay method |
| CN211402399U (en) * | 2019-08-21 | 2020-09-01 | 河北英茂生物科技有限公司 | Enzyme-linked immunoassay instrument for detecting beta-receptor stimulant |
| CN211856605U (en) * | 2020-03-18 | 2020-11-03 | 天津吉尼克生物科技有限公司 | Multifunctional enzyme-linked immunosorbent assay (ELISA) instrument for proteomics detection |
| CN214174400U (en) * | 2020-12-24 | 2021-09-10 | 青岛万明赛伯药业有限公司 | Enzyme-labeling instrument with universal supporting plate |
| CN216560625U (en) * | 2021-11-19 | 2022-05-17 | 郑州莱士血液制品有限公司 | Enzyme-linked immunosorbent assay (ELISA) instrument for detecting human serum albumin |
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