CN211856605U - Multifunctional enzyme-linked immunosorbent assay (ELISA) instrument for proteomics detection - Google Patents
Multifunctional enzyme-linked immunosorbent assay (ELISA) instrument for proteomics detection Download PDFInfo
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- CN211856605U CN211856605U CN202020340485.XU CN202020340485U CN211856605U CN 211856605 U CN211856605 U CN 211856605U CN 202020340485 U CN202020340485 U CN 202020340485U CN 211856605 U CN211856605 U CN 211856605U
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- 238000001514 detection method Methods 0.000 title claims abstract description 90
- 238000002965 ELISA Methods 0.000 title description 8
- 102000006833 Multifunctional Enzymes Human genes 0.000 title description 2
- 108010047290 Multifunctional Enzymes Proteins 0.000 title description 2
- 239000011148 porous material Substances 0.000 claims abstract description 13
- 239000011248 coating agent Substances 0.000 claims abstract description 10
- 238000000576 coating method Methods 0.000 claims abstract description 10
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims abstract description 8
- 229910052709 silver Inorganic materials 0.000 claims abstract description 8
- 239000004332 silver Substances 0.000 claims abstract description 8
- 239000011521 glass Substances 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims description 28
- 230000001681 protective effect Effects 0.000 claims description 15
- 238000007789 sealing Methods 0.000 claims description 10
- 239000000084 colloidal system Substances 0.000 claims 1
- 230000002093 peripheral effect Effects 0.000 claims 1
- 239000011550 stock solution Substances 0.000 claims 1
- 210000000439 stratum lucidum Anatomy 0.000 claims 1
- 239000003292 glue Substances 0.000 abstract description 7
- 238000000034 method Methods 0.000 description 4
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000029918 bioluminescence Effects 0.000 description 1
- 238000005415 bioluminescence Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000003760 hair shine Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
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Abstract
The utility model belongs to the technical field of ELIASA, in particular to a multifunctional ELIASA for proteomics detection, which comprises an ELIASA whole body, a detection disc, a protection door and a connecting groove, wherein the inner wall of one front side of the ELIASA whole body is embedded and connected with the detection disc, the periphery of the inner wall of the front side of the ELIASA whole body is embedded and connected with the connecting groove, the outer side of the bottom of the detection disc is embedded and connected with a support frame, the top of the inner side of the detection disc is embedded and connected with a pore plate, the other front side of the ELIASA whole body is movably connected with the protection door, the protection door can seal and protect the detection part on the ELIASA, and can also reduce the influence of the external environment on the instrument detection, the silver glue coating on the shielding plate can shield the ultraviolet ray in the external environment, the brown glass layer on the shielding plate can, the influence of the external environment on the detection work inside the instrument is reduced, and the working efficiency of the instrument detection is effectively improved.
Description
Technical Field
The utility model belongs to the technical field of the ELIASA specifically is a protein omics detects uses multi-functional ELIASA.
Background
Enzyme-linked immunosorbent assay (ELISA) instrument. Is a special instrument for enzyme-linked immunosorbent assay, also called as a micropore plate detector. The method can be simply divided into semi-automatic and full-automatic types, but the working principles are basically consistent, and the core of the method is a colorimeter, namely, a colorimetric method is used for analysis. The final volume of the test solution is generally required to be below 250 mu L, and the test cannot be completed by using a common photoelectric colorimeter, so that the method has special requirements on the photoelectric colorimeter in a microplate reader, and the type FLUOstarOPTIMA can be used for detecting fluorescence intensity, time-resolved fluorescence, ultraviolet visible absorption light, chemiluminescence and bioluminescence, but has certain defects.
The existing multifunctional microplate reader for proteomics detection has weak anti-interference performance and poor detection effect, ultraviolet rays have great influence on detection results, the amount of liquid to be detected is difficult to control, the existing multifunctional microplate reader for proteomics detection has no protection part, can not protect the part to be detected, is easily influenced by external environmental factors, the instrument is not perfect enough, the detection result of the microplate reader is influenced, and the requirement of the modern society is difficult to meet.
Therefore, how to design a multifunctional microplate reader for proteomics detection becomes a problem which needs to be solved currently.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a proteomics detects uses multi-functional ELIASA to it is weak to provide interference immunity in solving above-mentioned background art, and the not good problem of detection effect.
In order to achieve the above object, the utility model provides a following technical scheme: a multifunctional ELIASA for proteomics detection comprises an ELIASA whole body, a detection disc, a protection door and a connecting groove, wherein the detection disc is connected to the inner wall of one front side of the ELIASA whole body in an embedded manner, the connecting groove is connected to the periphery of the inner wall of the front side of the ELIASA whole body in an embedded manner, the protection door is movably connected to the other front side of the ELIASA whole body in a movable manner, one end of the protection door is connected with a movable shaft in an embedded manner, a shielding plate is connected to the middle position inside the protection door in an embedded manner, a supporting frame is connected to the outer side of the bottom of the detection disc in an embedded manner, a pore plate is connected to the top of the inner side of the detection disc in an embedded manner, a detection hole is connected to the inner wall of the middle inner bottom of the pore plate in an embedded, the periphery of the other end of the protective door is fixedly connected with a sealing ring, the inner wall of the middle of the interior of the detection disc is connected with a fixing hole in an embedded mode, the middle of the interior of the detection disc is fixedly connected with a transparent layer, and the other side of the bottom of the shielding plate is fixedly connected with a brown glass layer.
Preferably, a silver glue coating is embedded and connected to one side of the bottom of the shielding plate.
Preferably, a strong magnetic block is embedded and connected to the other side of the front face of the protection door.
Preferably, the two sides of the bottom end of the whole microplate reader are fixedly connected with anti-skid support legs.
Preferably, the number of the detection plates is one, the detection plates are arranged on the inner wall of one side of the front of the whole microplate reader, and the detection plates are fixedly connected with the whole microplate reader.
Preferably, the protection door is arranged on the other side of the front of the whole microplate reader, and the protection door is movably connected with the whole microplate reader.
Compared with the prior art, the beneficial effects of the utility model are that:
1. proteomics detects uses multi-functional ELIASA, detects the dish setting at the holistic preceding one side inner wall of ELIASA, detects the dish and the whole fixed connection of ELIASA, detects the dish and can transmit the liquid that awaits measuring that is more than, ensures that every inspection hole on the orifice plate adds the appearance volume and strictly unanimously, reduces the degree of difficulty of instrument operation.
2. Proteomics detects uses multi-functional ELIASA, shutter setting are at the holistic preceding opposite side of ELIASA, shutter and the whole swing joint of ELIASA, and the shutter can seal the detection site on the ELIASA and protect, and can also reduce the influence of external environment to the instrument detection, the effectual work efficiency who improves the instrument detection.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a cross-sectional view of the test tray of the present invention;
FIG. 3 is a cross-sectional view taken along line A-A of the present invention;
fig. 4 is a cross-sectional view of the shielding plate of the present invention.
In the figure: 1. the ELISA reader is integrated, 2, a detection disc, 3, a protection door, 4, a connecting groove, 5, an anti-skidding supporting leg, 6, a movable shaft, 7, a strong magnetic block, 8, a supporting frame, 9, a pore plate, 10, a detection hole, 11, an arc diversion hole, 12, a liquid storage bin, 13, a liquid discharge hole, 14, a fixing hole, 15, a sealing ring, 16, a transparent layer, 17, a shielding plate, 18, a silver glue coating, 19 and a brown glass layer.
Detailed Description
The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, rather than all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative work belong to the protection scope of the present invention based on the embodiments of the present invention.
In a first embodiment, please refer to fig. 1-4, the present invention provides a technical solution: a multifunctional ELIASA for proteomics detection comprises an ELIASA whole body 1, a detection disc 2, a protection door 3 and a connecting groove 4, wherein the inner wall of one front side of the ELIASA whole body 1 is connected with the detection disc 2 in an embedded manner, the periphery of the inner wall of the front side of the ELIASA whole body 1 is connected with the connecting groove 4 in an embedded manner, the other front side of the ELIASA whole body 1 is movably connected with the protection door 3, one end of the protection door 3 is connected with a movable shaft 6 in an embedded manner, the middle position of the interior of the protection door 3 is connected with a baffle plate 17 in an embedded manner, the outer side of the bottom of the detection disc 2 is connected with a support frame 8 in an embedded manner, the top of the inner side of the detection disc 2 is connected with a pore plate 9 in an embedded manner, the middle inner wall of the pore plate 9 is connected with a detection hole 10 in an embedded, a sealing ring 15 is fixedly connected to the periphery of the other end of the protective door 3, a fixing hole 14 is embedded and connected to the inner middle wall of the interior of the detection disc 2, a transparent layer 16 is fixedly connected to the middle portion of the interior of the detection disc 2, and a brown glass layer 19 is fixedly connected to the other side of the bottom of the shielding plate 17.
Preferably, the bottom one side embedding of shielding plate 17 is connected with silver-colored glue coating 18, when treating through the ELIASA and detect the liquid and detect, the ultraviolet ray and the illumination of external environment can influence the result that detects and the accuracy that detects, and silver-colored glue coating 18 is the more smooth aspect, can reflect the ultraviolet ray that external environment shines for in the ultraviolet ray in the external environment can not get into the detection dish 2 in the ELIASA, the effectual interference immunity that has improved the ELIASA and detected time measuring.
Preferably, the preceding opposite side embedding of shutter 3 is connected with strong magnet 7, when using the ELIASA, need close shutter 3, strong magnet 7 is provided with two altogether, a setting is on shutter 3, another setting is on the whole 1 of ELIASA, when closing shutter 3, strong magnet 7 on shutter 3 corresponds and through magnetic force to attracting with the whole 1 strong magnet 7 of ELIASA, make when closing or opening shutter 3, do not need complicated operation to carry out the closure locking, the effectual convenience that improves the instrument and use.
Preferably, the whole 1 bottom both sides fixedly connected with antiskid supporting leg 5 of ELIASA, before using the instrument, need support the whole 1 of ELIASA through antiskid supporting leg 5, antiskid supporting leg 5's bottom is provided with coarse line, make when antiskid supporting leg 5's bottom and the stationary plane contact of instrument, increase antiskid supporting leg 5's bottom and the frictional force of stationary plane contact, the effectual stability that antiskid supporting leg 5 supported fixedly that has improved.
Preferably, one detection disc 2 is arranged, the detection disc 2 is arranged on the inner wall of one side of the front of the whole microplate reader 1, the detection disc 2 is fixedly connected with the whole microplate reader 1, the detection disc 2 can transmit more liquid to be detected, the sample adding amount of each detection hole 10 on the pore plate 9 is ensured to be strictly consistent, the difficulty of instrument operation is reduced, when the liquid to be detected is added into the detection hole 10 and is higher than the arc-shaped diversion hole 11, the liquid to be detected is transmitted into the liquid storage bin 12 through the arc-shaped flow guide holes 11, the liquid level in each detection hole 10 on the pore plate 9 is ensured to be the same, when cleaning the liquid to be detected in the liquid storage bin 12, the liquid can be discharged only by opening the sealing cover on the liquid discharge hole 13, the operation difficulty of the microplate reader during use is reduced, the liquid level of each detection hole 10 can be ensured to be the same by workers with insufficient experience, and the practicability of the instrument is effectively improved.
Preferably, the protection door 3 is provided with one, the protection door 3 is arranged at the other side of the front of the whole microplate reader 1, the protection door 3 is movably connected with the whole microplate reader 1, the protection door 3 can seal and protect the detection part on the microplate reader and can also reduce the influence of the external environment on the detection of the instrument, when the instrument is used, the protection door 3 is moved and closed leftwards through the movable shaft 6, the protection door 3 is fixed and closed through the strong magnet 7, the sealing ring 15 on the protection door 3 is embedded into the connecting groove 4 on the whole microplate reader 1, the position of the detection disc 12 is protected in a sealing way, the shielding plate 17 embedded into the fixing hole 14 of the protection door 3 shields ultraviolet rays and hard light of the external environment, the silver glue coating 18 on the shielding plate 17 can shield the ultraviolet rays in the external environment, the brown glass layer 19 on the shielding plate 17 can shield the hard light of the external environment, the influence of the external environment on the detection work inside the instrument is reduced, and the working efficiency of the instrument detection is effectively improved.
The working principle is as follows: the method comprises the following steps that firstly, a shielding plate 17 is checked or replaced, a silver adhesive coating 18 is embedded and connected to one side of the bottom of the shielding plate 17, when a liquid to be detected is detected through an microplate reader, ultraviolet rays and illumination of the external environment can affect the detection result and the detection accuracy, the silver adhesive coating 18 is a smooth layer, the ultraviolet rays irradiated by the external environment can be reflected, the ultraviolet rays in the external environment cannot enter a detection disc 2 in the microplate reader, and the anti-interference performance of the microplate reader during detection is effectively improved;
then, the instrument is supported and fixed through the anti-skid supporting legs 5, the anti-skid supporting legs 5 are fixedly connected to two sides of the bottom end of the whole enzyme-labeling instrument 1, before the instrument is used, the whole enzyme-labeling instrument 1 needs to be supported through the anti-skid supporting legs 5, rough grains are arranged at the bottom ends of the anti-skid supporting legs 5, when the bottom ends of the anti-skid supporting legs 5 are in contact with a fixing surface of the instrument, the friction force of the bottom ends of the anti-skid supporting legs 5 in contact with the fixing surface is increased, and the supporting and fixing stability of the anti-skid supporting legs 5 is effectively improved;
then, the protective door 3 is closed, one protective door 3 is arranged, the protective door 3 is arranged at the other side of the front of the whole microplate reader 1, the protective door 3 is movably connected with the whole microplate reader 1, the protective door 3 can seal and protect the detection part on the microplate reader and can also reduce the influence of the external environment on the detection of the instrument, when the instrument is used, the protective door 3 is moved and closed leftwards through the movable shaft 6, the protective door 3 is fixed and closed through the strong magnet 7, the sealing ring 15 on the protective door 3 is embedded into the connecting groove 4 on the whole microplate reader 1, the position of the detection disc 12 is protected in a sealing way, the shielding plate 17 embedded into the fixed hole 14 of the protective door 3 shields the ultraviolet rays and the strong light of the external environment, the silver glue coating 18 on the shielding plate 17 can shield the ultraviolet rays in the external environment, the brown glass layer 19 on the shielding plate 17 can shield the strong light of the external environment, the influence of the external environment on the detection work inside the instrument is reduced, and the working efficiency of the instrument detection is effectively improved;
next, the protection door 3 is locked by the strong magnet 7, the strong magnet 7 is embedded and connected to the other side of the front surface of the protection door 3, when the microplate reader is used, the protection door 3 needs to be closed, the strong magnets 7 are totally provided with two, one is arranged on the protection door 3, the other is arranged on the whole microplate reader 1, when the protection door 3 is closed, the strong magnet 7 on the protection door 3 corresponds to the strong magnet 7 on the whole microplate reader 1 and is attracted by magnetic force, so that when the protection door 3 is closed or opened, the locking is closed without complex operation, and the convenience of the use of the instrument is effectively improved;
finally, the liquid to be detected is detected through the detection disc 2, one detection disc 2 is arranged, the detection disc 2 is arranged on the inner wall of one side of the front of the whole ELIASA 1, the detection disc 2 is fixedly connected with the whole ELIASA 1, the detection disc 2 can transmit more liquid to be detected, the sample adding amount of each detection hole 10 on the pore plate 9 is ensured to be strictly consistent, the operation difficulty of the instrument is reduced, when the liquid to be detected is added into the detection hole 10 and exceeds the arc-shaped flow guide hole 11, the liquid to be detected is transmitted into the liquid storage bin 12 through the arc-shaped flow guide hole 11, the liquid level in each detection hole 10 on the pore plate 9 is ensured to be the same, when the liquid to be detected in the liquid storage bin 12 is cleaned, the liquid can be discharged only by opening the sealing cover on the liquid discharge hole 13, the operation difficulty when the ELIASA is used is reduced, and the liquid level of each detection hole 10 can be ensured to be the same by inex, the practicability of the instrument is effectively improved, and the multifunctional microplate reader for proteomics detection is the working principle.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. The utility model provides a proteomics detects uses multi-functional ELIASA, includes that ELIASA is whole (1), detects dish (2), shutter (3) and spread groove (4), its characterized in that: the inner wall of one side of the front of the whole microplate reader (1) is embedded and connected with a detection plate (2), the periphery of the front inner wall of the whole microplate reader (1) is embedded and connected with a connecting groove (4), the other side of the front of the whole microplate reader (1) is movably connected with a protective door (3), one end of the protective door (3) is connected with a movable shaft (6) in an embedded manner, the middle position in the protective door (3) is connected with a baffle plate (17) in an embedded manner, the outer side of the bottom of the detection disc (2) is connected with a support frame (8) in an embedded way, the top of the inner side of the detection disc (2) is embedded and connected with a pore plate (9), the middle inner wall of the pore plate (9) is embedded and connected with a detection hole (10), the inner wall of the bottom end inside the pore plate (9) is embedded and connected with a liquid storage bin (12), arc-shaped flow guide holes (11) are embedded and connected to the inner walls of the two sides of the top of the detection hole (10).
2. The multifunctional microplate reader for proteomics detection according to claim 1, which is characterized in that: the inner wall embedding is connected with outage (13) in the middle of the bottom of stock solution storehouse (12), the peripheral fixedly connected with sealing washer (15) of the other end of shutter (3), inner wall embedding is connected with fixed orifices (14) in the middle of the inside of test tray (2), the inside middle part fixedly connected with stratum lucidum (16) of test tray (2), the bottom opposite side fixedly connected with brown glass layer (19) of shielding plate (17).
3. The multifunctional microplate reader for proteomics detection according to claim 1, which is characterized in that: one side of the bottom of the shielding plate (17) is embedded and connected with a silver colloid coating (18).
4. The multifunctional microplate reader for proteomics detection according to claim 1, which is characterized in that: the other side of the front of the protective door (3) is embedded and connected with a strong magnetic block (7).
5. The multifunctional microplate reader for proteomics detection according to claim 1, which is characterized in that: and the two sides of the bottom end of the whole microplate reader (1) are fixedly connected with anti-skid support legs (5).
6. The multifunctional microplate reader for proteomics detection according to claim 1, which is characterized in that: the detection device is characterized in that one detection disc (2) is arranged, the detection disc (2) is arranged on the inner wall of one side of the front of the whole microplate reader (1), and the detection disc (2) is fixedly connected with the whole microplate reader (1).
7. The multifunctional microplate reader for proteomics detection according to claim 1, which is characterized in that: the protection door (3) is arranged on one side, the protection door (3) is arranged on the other side of the front of the whole microplate reader (1), and the protection door (3) is movably connected with the whole microplate reader (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020340485.XU CN211856605U (en) | 2020-03-18 | 2020-03-18 | Multifunctional enzyme-linked immunosorbent assay (ELISA) instrument for proteomics detection |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202020340485.XU CN211856605U (en) | 2020-03-18 | 2020-03-18 | Multifunctional enzyme-linked immunosorbent assay (ELISA) instrument for proteomics detection |
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| CN211856605U true CN211856605U (en) | 2020-11-03 |
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| CN202020340485.XU Expired - Fee Related CN211856605U (en) | 2020-03-18 | 2020-03-18 | Multifunctional enzyme-linked immunosorbent assay (ELISA) instrument for proteomics detection |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115032151A (en) * | 2022-06-10 | 2022-09-09 | 杨富松 | Efficient multifunctional enzyme-labeling instrument and use method thereof |
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2020
- 2020-03-18 CN CN202020340485.XU patent/CN211856605U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115032151A (en) * | 2022-06-10 | 2022-09-09 | 杨富松 | Efficient multifunctional enzyme-labeling instrument and use method thereof |
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