CN115003829A - 带有修饰的核苷酸的闭合线性dna - Google Patents

带有修饰的核苷酸的闭合线性dna Download PDF

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CN115003829A
CN115003829A CN202180011940.9A CN202180011940A CN115003829A CN 115003829 A CN115003829 A CN 115003829A CN 202180011940 A CN202180011940 A CN 202180011940A CN 115003829 A CN115003829 A CN 115003829A
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dna
cldna
nucleotides
sequence
interest
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J·奥亚萨瓦尔圣马里纳
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Tyrese Therapeutics
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Tyrese Therapeutics
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/7115—Nucleic acids or oligonucleotides having modified bases, i.e. other than adenine, guanine, cytosine, uracil or thymine
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
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    • A61K31/713—Double-stranded nucleic acids or oligonucleotides
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/314—Phosphoramidates
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/323—Chemical structure of the sugar modified ring structure
    • C12N2310/3231—Chemical structure of the sugar modified ring structure having an additional ring, e.g. LNA, ENA
    • C—CHEMISTRY; METALLURGY
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/33—Chemical structure of the base
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/532—Closed or circular

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  • Proteomics, Peptides & Aminoacids (AREA)
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  • Animal Behavior & Ethology (AREA)
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  • Crystallography & Structural Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN202180011940.9A 2020-01-31 2021-01-29 带有修饰的核苷酸的闭合线性dna Pending CN115003829A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20382063 2020-01-31
EP20382063.4 2020-01-31
PCT/EP2021/052204 WO2021152147A1 (en) 2020-01-31 2021-01-29 Closed linear dna with modified nucleotides

Publications (1)

Publication Number Publication Date
CN115003829A true CN115003829A (zh) 2022-09-02

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US (1) US20230323343A1 (https=)
EP (1) EP4097253A1 (https=)
JP (2) JP2023511992A (https=)
KR (1) KR20220133999A (https=)
CN (1) CN115003829A (https=)
AU (1) AU2021213927A1 (https=)
CA (1) CA3164390A1 (https=)
WO (1) WO2021152147A1 (https=)

Cited By (1)

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WO2026012450A1 (zh) * 2024-07-11 2026-01-15 石药集团中奇制药技术（石家庄）有限公司 一种非病毒载体介导的大片段dna定点敲入系统

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EP4083227A1 (en) * 2021-04-29 2022-11-02 4basebio, S.L.U. Linear dna with enhanced resistance against exonucleases
EP4124660A1 (en) * 2021-07-30 2023-02-01 4basebio, S.L.U. Linear dna with enhanced resistance against exonucleases and methods for the production thereof
EP4388118A4 (en) * 2021-08-19 2025-05-21 Twist Bioscience Corporation METHODS AND COMPOSITIONS IN COMPOSITION WITH COVALENTLY CLOSED NUCLEIC ACIDS
CN114075595B (zh) * 2021-11-22 2023-11-17 上海交通大学 甲基化检测组合物、试剂盒及方法
JP2025507359A (ja) * 2022-02-10 2025-03-18 コード バイオセラピューティクス インコーポレイテッド 増強されたｄｎａデンドリマー及びその使用方法
JP2025516638A (ja) * 2022-05-13 2025-05-30 フラッグシップ パイオニアリング イノベーションズ セブン，エルエルシー 二本鎖ｄｎａ組成物及び関連する方法
CN115976138A (zh) * 2022-06-02 2023-04-18 宜明(苏州)细胞生物科技有限公司 新型闭合线性双链dna的体外制备技术
WO2024039652A1 (en) 2022-08-16 2024-02-22 Aldevron, L.L.C. Cell-free method of producing synthetic circular nucleic acid
ES3062031T3 (https=) * 2023-02-01 2026-04-08 4Basebio Uk Ltd
EP4410995A1 (en) * 2023-02-01 2024-08-07 4basebio, S.L.U. Linear dna with enhanced resistance against exonucleases and methods for the production thereof
WO2025034978A1 (en) * 2023-08-09 2025-02-13 Code Biotherapeutics, Inc. Enhanced dna dendrimers and methods of use thereof
WO2025064396A1 (en) * 2023-09-18 2025-03-27 Intellia Therapeutics, Inc. Nuclease resistant double stranded dna product for non-viral delivery to a cell and methods of production thereof
WO2025096807A2 (en) * 2023-10-31 2025-05-08 Flagship Pioneering Innovations Vii, Llc Novel therapeutic dna forms
WO2025202929A1 (en) * 2024-03-29 2025-10-02 Pfizer Inc. Methods for producing nucleic acids
EP4663200A1 (en) * 2024-06-10 2025-12-17 CPTx GmbH Compositions and methods

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CN1918293A (zh) * 2004-02-20 2007-02-21 莫洛根股份公司 用于对人及高等动物进行治疗性和预防性免疫刺激的取代的非编码核酸分子
CN105264074A (zh) * 2014-02-18 2016-01-20 莫洛根股份公司 共价闭合的非编码免疫调节性dna构建体
CN106460065A (zh) * 2014-04-11 2017-02-22 雷德沃特生物科学公司 用于基因组应用和治疗应用的核酸分子的克隆复制和扩增的系统和方法
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2026012450A1 (zh) * 2024-07-11 2026-01-15 石药集团中奇制药技术（石家庄）有限公司 一种非病毒载体介导的大片段dna定点敲入系统

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CA3164390A1 (en) 2021-08-05
JP2025186257A (ja) 2025-12-23
JP2023511992A (ja) 2023-03-23
KR20220133999A (ko) 2022-10-05
AU2021213927A1 (en) 2022-07-28
EP4097253A1 (en) 2022-12-07
WO2021152147A1 (en) 2021-08-05
US20230323343A1 (en) 2023-10-12

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