CA3164390A1 - Closed linear dna with modified nucleotides - Google Patents
Closed linear dna with modified nucleotides Download PDFInfo
- Publication number
- CA3164390A1 CA3164390A1 CA3164390A CA3164390A CA3164390A1 CA 3164390 A1 CA3164390 A1 CA 3164390A1 CA 3164390 A CA3164390 A CA 3164390A CA 3164390 A CA3164390 A CA 3164390A CA 3164390 A1 CA3164390 A1 CA 3164390A1
- Authority
- CA
- Canada
- Prior art keywords
- dna
- cidna
- nucleotide
- interest
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7088—Compounds having three or more nucleosides or nucleotides
- A61K31/7115—Nucleic acids or oligonucleotides having modified bases, i.e. other than adenine, guanine, cytosine, uracil or thymine
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7088—Compounds having three or more nucleosides or nucleotides
- A61K31/713—Double-stranded nucleic acids or oligonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/31—Chemical structure of the backbone
- C12N2310/314—Phosphoramidates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/32—Chemical structure of the sugar
- C12N2310/323—Chemical structure of the sugar modified ring structure
- C12N2310/3231—Chemical structure of the sugar modified ring structure having an additional ring, e.g. LNA, ENA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/33—Chemical structure of the base
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/50—Physical structure
- C12N2310/53—Physical structure partially self-complementary or closed
- C12N2310/531—Stem-loop; Hairpin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/50—Physical structure
- C12N2310/53—Physical structure partially self-complementary or closed
- C12N2310/532—Closed or circular
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Immunology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Analytical Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP20382063 | 2020-01-31 | ||
| EP20382063.4 | 2020-01-31 | ||
| PCT/EP2021/052204 WO2021152147A1 (en) | 2020-01-31 | 2021-01-29 | Closed linear dna with modified nucleotides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3164390A1 true CA3164390A1 (en) | 2021-08-05 |
Family
ID=69713977
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3164390A Pending CA3164390A1 (en) | 2020-01-31 | 2021-01-29 | Closed linear dna with modified nucleotides |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20230323343A1 (https=) |
| EP (1) | EP4097253A1 (https=) |
| JP (2) | JP2023511992A (https=) |
| KR (1) | KR20220133999A (https=) |
| CN (1) | CN115003829A (https=) |
| AU (1) | AU2021213927A1 (https=) |
| CA (1) | CA3164390A1 (https=) |
| WO (1) | WO2021152147A1 (https=) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4083227A1 (en) * | 2021-04-29 | 2022-11-02 | 4basebio, S.L.U. | Linear dna with enhanced resistance against exonucleases |
| EP4124660A1 (en) * | 2021-07-30 | 2023-02-01 | 4basebio, S.L.U. | Linear dna with enhanced resistance against exonucleases and methods for the production thereof |
| EP4388118A4 (en) * | 2021-08-19 | 2025-05-21 | Twist Bioscience Corporation | METHODS AND COMPOSITIONS IN COMPOSITION WITH COVALENTLY CLOSED NUCLEIC ACIDS |
| CN114075595B (zh) * | 2021-11-22 | 2023-11-17 | 上海交通大学 | 甲基化检测组合物、试剂盒及方法 |
| JP2025507359A (ja) * | 2022-02-10 | 2025-03-18 | コード バイオセラピューティクス インコーポレイテッド | 増強されたｄｎａデンドリマー及びその使用方法 |
| JP2025516638A (ja) * | 2022-05-13 | 2025-05-30 | フラッグシップ パイオニアリング イノベーションズ セブン，エルエルシー | 二本鎖ｄｎａ組成物及び関連する方法 |
| CN115976138A (zh) * | 2022-06-02 | 2023-04-18 | 宜明(苏州)细胞生物科技有限公司 | 新型闭合线性双链dna的体外制备技术 |
| WO2024039652A1 (en) | 2022-08-16 | 2024-02-22 | Aldevron, L.L.C. | Cell-free method of producing synthetic circular nucleic acid |
| ES3062031T3 (https=) * | 2023-02-01 | 2026-04-08 | 4Basebio Uk Ltd | |
| EP4410995A1 (en) * | 2023-02-01 | 2024-08-07 | 4basebio, S.L.U. | Linear dna with enhanced resistance against exonucleases and methods for the production thereof |
| WO2025034978A1 (en) * | 2023-08-09 | 2025-02-13 | Code Biotherapeutics, Inc. | Enhanced dna dendrimers and methods of use thereof |
| WO2025064396A1 (en) * | 2023-09-18 | 2025-03-27 | Intellia Therapeutics, Inc. | Nuclease resistant double stranded dna product for non-viral delivery to a cell and methods of production thereof |
| WO2025096807A2 (en) * | 2023-10-31 | 2025-05-08 | Flagship Pioneering Innovations Vii, Llc | Novel therapeutic dna forms |
| WO2025202929A1 (en) * | 2024-03-29 | 2025-10-02 | Pfizer Inc. | Methods for producing nucleic acids |
| EP4663200A1 (en) * | 2024-06-10 | 2025-12-17 | CPTx GmbH | Compositions and methods |
| WO2026012450A1 (zh) * | 2024-07-11 | 2026-01-15 | 石药集团中奇制药技术（石家庄）有限公司 | 一种非病毒载体介导的大片段dna定点敲入系统 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4373071A (en) | 1981-04-30 | 1983-02-08 | City Of Hope Research Institute | Solid-phase synthesis of polynucleotides |
| JP4517061B2 (ja) * | 2003-08-08 | 2010-08-04 | 独立行政法人産業技術総合研究所 | ダンベル型ｄｎａの効率的な製造方法 |
| CN1918293A (zh) * | 2004-02-20 | 2007-02-21 | 莫洛根股份公司 | 用于对人及高等动物进行治疗性和预防性免疫刺激的取代的非编码核酸分子 |
| WO2006085664A1 (ja) | 2005-02-10 | 2006-08-17 | The University Of Tokyo | ポリカチオン荷電性ポリマー及び核酸のキャリヤーとしての使用 |
| ES2359058B1 (es) | 2009-07-02 | 2012-03-27 | Consejo Superior De Investigaciones Cient�?Ficas (Csic) | Quimera de adn polimerasa del fago ph1 29. |
| GB2523187A (en) * | 2014-02-18 | 2015-08-19 | Mologen Ag | Covalently closed non-coding immunomodulatory DNA construct |
| US20170067097A1 (en) * | 2014-04-11 | 2017-03-09 | Redvault Biosciences, Lp | Systems and methods for clonal replication and amplification of nucleic acid molecules for genomic and therapeutic applications |
| GB201418159D0 (en) * | 2014-10-14 | 2014-11-26 | Oxford Nanopore Tech Ltd | Method |
| DK3500682T3 (da) * | 2016-08-16 | 2020-08-24 | Touchlight Ip Ltd | Fremstilling af lukket, lineært dna |
| US11473090B2 (en) * | 2017-05-16 | 2022-10-18 | Helix Nanotechnologies, Inc. | Linear covalently closed vectors and related compositions and methods thereof |
| EP3714064B8 (en) * | 2017-11-21 | 2024-10-30 | 4basebio UK Ltd | Methods and kits for amplification of double stranded dna |
-
2021
- 2021-01-29 CA CA3164390A patent/CA3164390A1/en active Pending
- 2021-01-29 US US17/796,532 patent/US20230323343A1/en active Pending
- 2021-01-29 CN CN202180011940.9A patent/CN115003829A/zh active Pending
- 2021-01-29 AU AU2021213927A patent/AU2021213927A1/en active Pending
- 2021-01-29 WO PCT/EP2021/052204 patent/WO2021152147A1/en not_active Ceased
- 2021-01-29 EP EP21703640.9A patent/EP4097253A1/en active Pending
- 2021-01-29 KR KR1020227030065A patent/KR20220133999A/ko active Pending
- 2021-01-29 JP JP2022545423A patent/JP2023511992A/ja active Pending
-
2025
- 2025-08-21 JP JP2025137441A patent/JP2025186257A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JP2025186257A (ja) | 2025-12-23 |
| JP2023511992A (ja) | 2023-03-23 |
| KR20220133999A (ko) | 2022-10-05 |
| AU2021213927A1 (en) | 2022-07-28 |
| EP4097253A1 (en) | 2022-12-07 |
| WO2021152147A1 (en) | 2021-08-05 |
| CN115003829A (zh) | 2022-09-02 |
| US20230323343A1 (en) | 2023-10-12 |
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