SG11201608486SA - Systems and methods for clonal replication and amplification of nucleic acid molecules for genomic and therapeutic applications - Google Patents

Systems and methods for clonal replication and amplification of nucleic acid molecules for genomic and therapeutic applications

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Publication number
SG11201608486SA
SG11201608486SA SG11201608486SA SG11201608486SA SG11201608486SA SG 11201608486S A SG11201608486S A SG 11201608486SA SG 11201608486S A SG11201608486S A SG 11201608486SA SG 11201608486S A SG11201608486S A SG 11201608486SA SG 11201608486S A SG11201608486S A SG 11201608486SA
Authority
SG
Singapore
Prior art keywords
genomic
amplification
systems
methods
nucleic acid
Prior art date
Application number
SG11201608486SA
Inventor
Michael L Metzker
Christopher August Weier
Original Assignee
Redvault Biosciences Lp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Redvault Biosciences Lp filed Critical Redvault Biosciences Lp
Publication of SG11201608486SA publication Critical patent/SG11201608486SA/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/507—Recombinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/513—Winding/unwinding enzyme, e.g. helicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/519—Topoisomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2522/00—Reaction characterised by the use of non-enzymatic proteins
    • C12Q2522/10—Nucleic acid binding proteins
    • C12Q2522/101—Single or double stranded nucleic acid binding proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/179—Modifications characterised by incorporating arbitrary or random nucleotide sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/125—Rolling circle
SG11201608486SA 2014-04-11 2015-04-11 Systems and methods for clonal replication and amplification of nucleic acid molecules for genomic and therapeutic applications SG11201608486SA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461978823P 2014-04-11 2014-04-11
PCT/US2015/025492 WO2015157747A1 (en) 2014-04-11 2015-04-11 Systems and methods for clonal replication and amplification of nucleic acid molecules for genomic and therapeutic applications

Publications (1)

Publication Number Publication Date
SG11201608486SA true SG11201608486SA (en) 2016-11-29

Family

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Family Applications (2)

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SG10201808952WA SG10201808952WA (en) 2014-04-11 2015-04-11 Systems and methods for clonal replication and amplification of nucleic acid molecules for genomic and therapeutic applications
SG11201608486SA SG11201608486SA (en) 2014-04-11 2015-04-11 Systems and methods for clonal replication and amplification of nucleic acid molecules for genomic and therapeutic applications

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SG10201808952WA SG10201808952WA (en) 2014-04-11 2015-04-11 Systems and methods for clonal replication and amplification of nucleic acid molecules for genomic and therapeutic applications

Country Status (10)

Country Link
US (1) US20170067097A1 (en)
EP (1) EP3129505B1 (en)
JP (2) JP7058502B2 (en)
KR (1) KR102592367B1 (en)
CN (2) CN113528628A (en)
AU (1) AU2015243130B2 (en)
CA (1) CA2945358C (en)
DK (1) DK3129505T3 (en)
SG (2) SG10201808952WA (en)
WO (1) WO2015157747A1 (en)

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WO2018081666A1 (en) * 2016-10-28 2018-05-03 Silgentech Inc. Methods of single dna/rna molecule counting
SG11202004534RA (en) 2017-11-21 2020-06-29 4basebio Sl Methods and kits for amplification of double stranded dna
KR102131494B1 (en) * 2018-04-17 2020-07-07 건국대학교 산학협력단 Detection of Target Nucleic Acid Sequences by PCR-coupled Rolling Circle Amplification
CN109055488B (en) * 2018-08-17 2021-11-02 中山康源基因技术科技有限公司 Method for preparing long probe by using annular single-chain probe and application of long probe in gene chip production
EP3650559B1 (en) * 2018-11-08 2022-06-29 Siemens Healthcare GmbH Direct rna nanopore sequencing with help of a stem-loop polynucleotide
KR102618147B1 (en) * 2018-12-05 2023-12-27 이지아이 테크 (센젠) 컴퍼니 리미티드 Rotational ring amplification method, sequencing library preparation method, and manufactured DNA nanospheres
CN111378750A (en) * 2018-12-29 2020-07-07 北京福安华生物科技有限公司 Artificial mimic nucleic acid molecular beacon and kit for detecting rs885004 site polymorphism of SLC28A3 gene
GB201911421D0 (en) * 2019-08-09 2019-09-25 Dnae Diagnostics Ltd Methods of multiplexed isothermal amplification of nucleic acid sequences
CN111139289A (en) * 2019-12-25 2020-05-12 中国海洋大学 Constant-temperature rolling ring amplification method based on dumbbell ring template
CA3164390A1 (en) * 2020-01-31 2021-08-05 Tyris Therapeutics, S.L. Closed linear dna with modified nucleotides
WO2023049937A2 (en) * 2021-09-27 2023-03-30 Intergalactic Therapeutics, Inc. Synthetic production of circular dna vectors

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US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
CA2360342A1 (en) * 1999-12-02 2001-06-07 Molecular Staging Inc. Generation of single-strand circular dna from linear self-annealing segments
WO2004065582A2 (en) * 2003-01-15 2004-08-05 Dana-Farber Cancer Institute, Inc. Amplification of dna in a hairpin structure, and applications
WO2005014810A1 (en) * 2003-08-08 2005-02-17 National Institute Of Advanced Industrial Science And Technology Efficient process for producing dumbbell dna
WO2005059157A2 (en) * 2003-12-11 2005-06-30 The Board Of Trustees Of The Leland Stanford Junior University METHODS AND COMPOSITIONS FOR USE IN PREPARING HAIRPIN RNAs
GB2413796B (en) * 2004-03-25 2006-03-29 Global Genomics Ab Methods and means for nucleic acid sequencing
ATE457363T1 (en) * 2004-12-29 2010-02-15 Applied Biosystems Llc METHODS, COMPOSITIONS AND KITS FOR FORMING COMPLEMENTARY POLYNUCLEOTIDES
KR101414713B1 (en) * 2007-10-11 2014-07-03 삼성전자주식회사 Method of amplifying target nucleic acids by rolling circle amplification in the presence of ligase and endonuclease
BRPI0909212A2 (en) * 2008-03-28 2015-08-18 Pacific Biosciences California Compositions and method for nucleic acid sequencing
US20110196142A1 (en) * 2008-09-04 2011-08-11 Biotechnology Research Institute The Chinese Academy of Agricultural Sciences DNA molecule for expressing Hairpin RNA, the constructing method and the use thereof
GB0906130D0 (en) 2008-10-03 2009-05-20 Procrata Biosystems Ltd Transcription factor decoys
US9267168B2 (en) * 2012-06-12 2016-02-23 Pacific Biosciences Of California, Inc. Methods and compositions for isolating template nucleic acids
US9416405B2 (en) * 2012-11-02 2016-08-16 Life Technologies Corporation Compositions, methods and kits for enhancing PCR specificity

Also Published As

Publication number Publication date
CA2945358A1 (en) 2015-10-15
JP2020188774A (en) 2020-11-26
JP7058502B2 (en) 2022-04-22
JP7100680B2 (en) 2022-07-13
CN113528628A (en) 2021-10-22
CA2945358C (en) 2022-12-06
KR20160138579A (en) 2016-12-05
EP3129505A1 (en) 2017-02-15
WO2015157747A1 (en) 2015-10-15
KR102592367B1 (en) 2023-10-26
US20170067097A1 (en) 2017-03-09
SG10201808952WA (en) 2018-11-29
AU2015243130B2 (en) 2021-08-26
EP3129505B1 (en) 2021-06-30
EP3129505A4 (en) 2017-12-06
CN106460065A (en) 2017-02-22
DK3129505T3 (en) 2021-10-04
JP2017512071A (en) 2017-05-18
AU2015243130A1 (en) 2016-11-03
WO2015157747A9 (en) 2016-08-25

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