CN114958656A - Biological agent and method for treating livestock and poultry urine and biogas slurry - Google Patents
Biological agent and method for treating livestock and poultry urine and biogas slurry Download PDFInfo
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- CN114958656A CN114958656A CN202210493816.7A CN202210493816A CN114958656A CN 114958656 A CN114958656 A CN 114958656A CN 202210493816 A CN202210493816 A CN 202210493816A CN 114958656 A CN114958656 A CN 114958656A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention belongs to the technical field of biogas slurry treatment, and particularly relates to a biological microbial inoculum and a method for treating livestock urine and biogas slurry, which comprises the steps of mixing a compound microbial inoculum with brown sugar water, uniformly stirring, and rejuvenating for 12-24h to obtain a compound microbial inoculum solution; conveying the livestock and poultry manure biogas slurry wastewater to a biogas slurry deposition tank for precipitation separation to remove suspended matters in the biogas slurry; sprinkling the compound microbial inoculum solution into the biogas slurry, opening an air pump regulating valve and controlling the flow to be 2 multiplied by 2L/min, and carrying out intermittent aeration culture for 72-96 h; conveying the biogas slurry obtained in the step into a microalgae biological purification tank for microalgae cultivation and purification, and finally discharging the biogas slurry up to the standard; the method combines the microbial agent with the aerobic treatment process of the sewage, utilizes high-efficiency microorganisms to quickly decompose pollutants, achieves the effects of decoloring and deodorizing, can achieve standard emission after disinfection treatment, and achieves the purposes of energy conservation, emission reduction, low cost and easy popularization and application.
Description
Technical Field
The invention belongs to the technical field of biogas slurry treatment, and particularly relates to a biological microbial agent and a method for treating livestock and poultry urine and biogas slurry.
Background
The biogas slurry is a brown bright liquid formed by fermenting organic substances and contains various amino acids, vitamins, proteins, auxin, saccharides, nucleic acid, antibiotics and the like. The biogas slurry is mainly used for seed soaking, foliage fertilizer spraying, nutrient soil mixing, flower and fruit retention, soilless culture mother solution, flower planting and the like. However, the biogas slurry also contains substances such as NH3, PO and the like which pollute the environment, and if the substances are not treated and discharged, the substances can pollute the environmental water body.
At present, few methods for treating livestock and poultry manure and biogas slurry are involved, while the traditional treatment of biogas slurry is natural ecological purification, and the other is industrial treatment, however, the former needs a large amount of land occupation and a long time, and the latter needs high cost, and the two methods have some defects in the treatment of livestock and poultry manure and biogas slurry, and are still not ideal enough in the treatment of livestock and poultry manure and biogas slurry, so that a method for treating livestock and poultry manure and biogas slurry is needed to improve the treatment effect of livestock and poultry manure and biogas slurry, so that the method has the advantages of saving the purification treatment time and improving the purification treatment effect.
Disclosure of Invention
In order to solve the defects in the prior art, the invention aims to provide a biological agent and a method for treating livestock urine and biogas slurry, the method combines a microbial agent and a sewage aerobic treatment process, pollutants are quickly decomposed by using high-efficiency microorganisms, the effects of decoloring and deodorizing are achieved, standard discharge can be realized after disinfection treatment, energy conservation and emission reduction are realized, the cost is low, and the method is easy to popularize and apply.
The technical scheme adopted by the invention is as follows:
a biological agent and a method for treating livestock and poultry urine and biogas slurry are provided, wherein the biological agent is obtained through the following steps:
s1: mixing actinomycete strains, lactobacillus strains, saccharomycete strains and aspergillus niger strains to obtain mixed strains;
s2: inoculating the mixed strain in an activation culture medium under the closed condition at the temperature of 20-35 ℃, performing activation culture for 24-48h, fermenting and propagating the cultured mixed strain in a production fermentation culture medium for 24-72h, and continuously shaking or stirring in the processes of the activation culture and the fermentation propagation to obtain a mixed bacterial liquid;
s3: adsorbing the mixed bacteria liquid by a diatomite carrier, drying and crushing to obtain composite bacteria powder;
s4: and uniformly mixing the composite microbial powder with the ground calcium carbonate, the drying agent and the trace elements to obtain the composite microbial agent.
As a further technical scheme, the mixing mass ratio of the actinomycete strains, the lactobacillus strains, the yeast and the aspergillus niger strains in the step S1 is 3: 1: 2: 1.
as a further technical scheme, in the step S2, the production fermentation medium comprises the following components in a mass fraction ratio of 2.5%: 5.0%: 0.5%: 0.8% ammonium chloride, sodium bicarbonate, calcium sulfate dihydrate, and magnesium sulfate heptahydrate.
As a further technical scheme, the mass percentage of each raw material in the step S4 is as follows: 10-70% of composite bacterial powder, 2-10% of trace elements, 5-20% of calcium carbonate and 5-10% of drying agent.
As a further technical scheme, the trace elements consist of zinc sulfate, magnesium sulfate and borax.
As a further technical scheme, the drying agent is one of bentonite, silica gel, white carbon black or attapulgite.
A biological agent and a method for treating livestock and poultry urine and biogas slurry comprise the following steps:
s1: mixing the compound microbial agent with brown sugar water, uniformly stirring, and rejuvenating for 12-24h to obtain a compound microbial agent solution;
s2: conveying the livestock and poultry manure biogas slurry wastewater to a biogas slurry deposition tank for precipitation separation to remove suspended matters in the biogas slurry;
s3: and (3) sprinkling the compound microbial inoculum solution into the biogas slurry, opening an air pump regulating valve, controlling the flow to be 2 multiplied by 2L/min, and carrying out intermittent aeration culture for 72-96 h.
S4: and (4) conveying the biogas slurry obtained in the step (S3) to a microalgae biological purification tank for microalgae cultivation and purification, and finally discharging the biogas slurry up to the standard.
As a further technical scheme, the gap aeration operation is specifically 14h aeration +10h standing, and circulating in sequence.
In summary, compared with the prior art, the invention has the beneficial effects that:
the production method of the compound microbial agent provided by the invention has fewer fermentation steps and easy process improvement, and the compound microbial agent realizes a good removal effect on BOD, ammonia nitrogen, total phosphorus and fecal coliform groups in the biogas slurry, obviously improves the water quality of the biogas slurry and enables the biogas slurry to reach the irrigation standard; the treatment method for treating the urine and the biogas slurry of the livestock and poultry by using the compound microbial agent can well remove total phosphorus and faecal coliform in the biogas slurry through intermittent exposure treatment, can promote the proper development of associated aerobic and anaerobic bacteria in the wastewater, paves a cushion for later microbial flora to obtain a small molecular organic matrix required by growth and reproduction and improve the purification effect and shorten the purification period, then accesses the compound microbial flora for proper aeration, treats the livestock and poultry urine and the biogas slurry through a good symbiotic system and an efficient degradation effect, and further improves the purification effect of the biogas slurry.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions of the embodiments of the present application will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present application without making any creative effort, shall fall within the protection scope of the present application.
The present invention will be described in detail
Example 1:
mixing actinomycete strains, lactobacillus strains, saccharomycete strains and aspergillus niger strains in a mass ratio of 3: 1: 2: 1, mixing to obtain mixed strains; inoculating the mixed strain in an activation culture medium under a closed condition at the temperature of 25 ℃, performing activation culture for 24 hours, then performing fermentation propagation on the cultured mixed strain in a production fermentation culture medium for 24 hours, and continuously shaking or stirring in the processes of the activation culture and the fermentation propagation to obtain a mixed bacterial liquid; adsorbing the mixed bacteria liquid by a diatomite carrier, drying and crushing to obtain composite bacteria powder; mixing the composite bacterial powder with crushed calcium carbonate, drying agent and trace elements according to the mass fraction ratio of 65%: 8%: 20%: 7 percent of the mixture is evenly mixed to obtain the compound microbial agent.
As a further technical scheme, in the step S2, the production fermentation medium comprises the following components in a mass fraction ratio of 2.5%: 5.0%: 0.5%: 0.8% ammonium chloride, sodium bicarbonate, calcium sulfate dihydrate, and magnesium sulfate heptahydrate.
As a further technical scheme, the trace elements consist of zinc sulfate, magnesium sulfate and borax.
As a further technical scheme, the drying agent is one of bentonite, silica gel, white carbon black or attapulgite.
The invention also provides a method for treating livestock urine and biogas slurry based on the compound microbial agent, which comprises the following steps:
s1: mixing the compound microbial agent with brown sugar water, uniformly stirring, and rejuvenating for 24h to obtain a compound microbial agent solution;
s2: conveying the livestock and poultry manure biogas slurry wastewater to a biogas slurry deposition tank for precipitation separation to remove suspended matters in the biogas slurry;
s3: and (3) sprinkling the compound microbial inoculum solution into the biogas slurry, opening an air pump regulating valve, controlling the flow to be 2 multiplied by 2L/min, and carrying out intermittent aeration culture for 72-96 h.
S4: and (4) conveying the biogas slurry obtained in the step (S3) to a microalgae biological purification tank for microalgae cultivation and purification, and finally discharging the biogas slurry up to the standard.
As a further technical scheme, the gap aeration operation is specifically 14h aeration +10h standing, and circulating in sequence.
Example 2:
mixing actinomycete strains, lactobacillus strains, saccharomycete strains and aspergillus niger strains in a mass ratio of 3: 1: 2: 1, mixing to obtain a mixed strain; inoculating the mixed strain in an activation culture medium under the closed condition at the temperature of 35 ℃, performing activation culture for 36h, then performing fermentation propagation on the cultured mixed strain in a production fermentation culture medium for 48h, and continuously shaking or stirring in the processes of the activation culture and the fermentation propagation to obtain a mixed bacterial liquid; adsorbing the mixed bacteria liquid by a diatomite carrier, drying and crushing to obtain composite bacteria powder; the composite bacterial powder, the ground calcium carbonate, the drying agent and the trace elements are mixed according to the mass fraction ratio of 70%: 5%: 15%: and mixing 10% of the mixture evenly to obtain the compound microbial agent.
As a further technical scheme, in the step S2, the production fermentation medium comprises the following components in a mass fraction ratio of 2.5%: 5.0%: 0.5%: 0.8% ammonium chloride, sodium bicarbonate, calcium sulfate dihydrate, and magnesium sulfate heptahydrate.
As a further technical scheme, the trace elements consist of zinc sulfate, magnesium sulfate and borax.
As a further technical scheme, the drying agent is one of bentonite, silica gel, white carbon black or attapulgite.
The invention also provides a method for treating livestock urine and biogas slurry based on the compound microbial agent, which comprises the following steps:
s1: mixing the compound microbial agent with brown sugar water, uniformly stirring, and rejuvenating for 24h to obtain a compound microbial agent solution;
s2: conveying the livestock and poultry manure biogas slurry wastewater to a biogas slurry deposition tank for precipitation separation to remove suspended matters in the biogas slurry;
s3: and (3) sprinkling the compound microbial inoculum solution into the biogas slurry, opening an air pump regulating valve, controlling the flow to be 2 multiplied by 2L/min, and carrying out intermittent aeration culture for 72-96 h.
S4: and (4) conveying the biogas slurry obtained in the step (S3) to a microalgae biological purification tank for microalgae cultivation and purification, and finally discharging the biogas slurry up to the standard.
As a further technical scheme, the gap aeration operation is specifically 14h aeration +10h standing, and circulating in sequence.
Comparative example 1:
unlike the above examples 1 and 2, the conventional biogas slurry treatment process (anaerobic digestion, biogas fermentation) was used in comparative example 1.
The above examples 1 and 2 and comparative example 1 were subjected to a sewage detection test:
immediately after the test, samples were taken for analysis, indices and measurements as given in Table 1.
| Measurement index | Index measuring method | Source of process |
| Biological oxygen demand BOD5. | Dilution and inoculation method | GB/T 7488-87 |
| Chemical oxygen demand CODc | Potassium dichromate process | GB/T 11914-89 |
| Ammonia nitrogen content NH3-N | Colorimetric method using Nashi reagent | GB 7479-87 |
| Total phosphorus content TP | Ammonium tweezer spectrophotometry | GB 11893-89 |
| Total salt content TS | Gravimetric method | Soil agricultural chemical analysis method |
| Faecal coliform FC | Membrane filtering method | GB/T19524.2-2004/HJ/T347-2007 |
| pH | Acidimeter | Method for monitoring and analyzing Water and wastewater |
The germination rate and germination index determination method comprises the following steps: putting three pieces of filter paper with proper size into a clean and sterile 9cm culture dish, and putting 20 plump radish seeds on the filter paper in order; accurately absorbing 5mL of water sample diluent into a culture dish, placing the water sample diluent into an incubator for culture, culturing in the incubator at the temperature of 25 +/-1 ℃ under the dark condition, finishing the test when the germination rate of the control group seeds reaches 65 percent and the root length reaches 20mm (about 48h), measuring the germination rate and the root length of the seeds, simultaneously using deionized water as a blank control, and repeating each treatment for 3 times. The calculation formula is as follows:
the specific experimental results are shown in table 2:
as can be seen from tables 1 and 2, compared with the traditional anaerobic digestion and biogas fermentation treatment process in the existing comparative example, the composite microbial agent and the method for treating the urine and biogas slurry of the livestock and poultry in the embodiments 1 and 2 of the invention have better removal effects on BOD, total phosphorus, faecal coliform bacteria, COD, ammonia nitrogen and total salt content in the biogas slurry, and the indexes such as germination percentage, GI and the like are better than those in the comparative example 1.
Therefore, the compound microbial agent obtained by the invention can be proved to realize a good removal effect on BOD, ammonia nitrogen, total phosphorus and fecal coliform bacteria in the biogas slurry, and obviously improve the water quality of the biogas slurry to reach the irrigation standard; the treatment method for treating the urine and the biogas slurry of the livestock and poultry by using the compound microbial agent can well remove total phosphorus and faecal coliform in the biogas slurry through intermittent exposure treatment, can promote the proper development of associated aerobic and anaerobic bacteria in the wastewater, paves a cushion for later microbial flora to obtain a small molecular organic matrix required by growth and reproduction and improve the purification effect and shorten the purification period, then accesses the compound microbial flora for proper aeration, treats the livestock and poultry urine and the biogas slurry through a good symbiotic system and an efficient degradation effect, and further improves the purification effect of the biogas slurry.
The above-mentioned embodiments are only used for illustrating the technical solutions of the present invention, and not for limiting the same; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (8)
1. A biological agent and a method for treating livestock and poultry urine and biogas slurry are characterized in that the biological agent is obtained through the following steps:
s1: mixing actinomycete strains, lactobacillus strains, saccharomycete strains and aspergillus niger strains to obtain mixed strains;
s2: inoculating the mixed strain in an activation culture medium at the temperature of 20-35 ℃ under a closed condition, performing activation culture for 24-48h, then performing fermentation propagation on the cultured mixed strain in a production fermentation culture medium for 24-72h, and continuously shaking or stirring in the processes of the activation culture and the fermentation propagation to obtain a mixed bacterial liquid;
s3: adsorbing the mixed bacteria liquid by a diatomite carrier, drying and crushing to obtain composite bacteria powder;
s4: and uniformly mixing the composite microbial powder with the ground calcium carbonate, the drying agent and the trace elements to obtain the composite microbial agent.
2. The biological agent and the method for treating livestock urine and biogas slurry according to claim 1, wherein the mixing mass ratio of the actinomycete strains, the lactobacillus strains, the saccharomycetes and the aspergillus niger strains in step S1 is 3: 1: 2: 1.
3. the biological agent and the method for treating livestock urine and biogas slurry according to claim 1, wherein the fermentation medium for production in step S2 comprises the following components in percentage by mass of 2.5%: 5.0%: 0.5%: 0.8% ammonium chloride, sodium bicarbonate, calcium sulfate dihydrate, and magnesium sulfate heptahydrate.
4. The biological agent and the method for treating livestock urine and biogas slurry according to claim 1, wherein the raw materials in step S4 comprise the following components in percentage by mass: 10-70% of composite bacterial powder, 2-10% of trace elements, 5-20% of calcium carbonate and 5-10% of drying agent.
5. The biological agent and the method for treating the urine and the biogas slurry of the livestock and the poultry according to claim 1, wherein the trace elements consist of zinc sulfate, magnesium sulfate and borax.
6. The biological agent and the method for treating livestock and poultry urine and biogas slurry according to claim 1, wherein the drying agent is one of bentonite, silica gel, white carbon black or attapulgite.
7. The biological agent and the method for treating the livestock and poultry urine and biogas slurry according to claim 1 are characterized by comprising the following steps:
s1: mixing the compound microbial agent with brown sugar water, uniformly stirring, and rejuvenating for 12-24h to obtain a compound microbial agent solution;
s2: conveying the livestock and poultry manure biogas slurry wastewater to a biogas slurry deposition tank for precipitation separation to remove suspended matters in the biogas slurry;
s3: sprinkling the compound microbial inoculum solution into the biogas slurry, opening an air pump regulating valve and controlling the flow to be 2 multiplied by 2L/min, and carrying out intermittent aeration culture for 72-96 h;
s4: and (4) conveying the biogas slurry obtained in the step (S3) to a microalgae biological purification tank for microalgae cultivation and purification, and finally discharging the biogas slurry up to the standard.
8. The biological agent and the method for treating the livestock and poultry urine and the biogas slurry according to claim 7, wherein the gap aeration operation is specifically aeration for 14h and standing for 10h, and the operation is circulated in sequence.
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