CN114949126A - Traditional Chinese medicine composition for promoting proliferation of beneficial bacteria in intestinal tract and preparation method and application thereof - Google Patents
Traditional Chinese medicine composition for promoting proliferation of beneficial bacteria in intestinal tract and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine composition for promoting the proliferation of beneficial bacteria in intestinal tracts and a preparation method and application thereof, and relates to the technical field of compositions. The invention provides a traditional Chinese medicine composition which comprises the following raw materials in parts by weight: 5-20 parts of coix seed, 1-15 parts of lotus seed, 1-10 parts of lucid ganoderma and 1-10 parts of Chinese date. The invention selects the medicinal and edible raw materials with good proliferation effect on beneficial bacteria in intestinal tracts (such as bifidobacterium and lactobacillus), and determines the mixture ratio of the raw materials through a large number of experimental screens, so that the combination ratio with obvious proliferation effect is selected, and the traditional Chinese medicine composition is obtained. The invention provides a traditional Chinese medicine composition which has wide raw material sources, and the prepared product has the advantages of strong stability, easy storage, low price and the like.
Description
Technical Field
The invention relates to the technical field of compositions, in particular to a traditional Chinese medicine composition for promoting the proliferation of beneficial bacteria in intestinal tracts, and a preparation method and application thereof.
Background
Coicis semen is dried mature kernel of Coix lacryma-jobi L.var.ma-yuen (Roman.) Stapf of Gramineae. Has effects of promoting diuresis, permeating moisture, invigorating spleen, relieving diarrhea, eliminating arthralgia, expelling pus, removing toxic substance and resolving hard mass. It can be used for treating edema, tinea pedis, dysuria, spleen deficiency, diarrhea, damp arthralgia, spasm, pulmonary abscess, intestinal abscess, wart, and cancer. Lotus seed, a dried mature seed of Nelumbo nucifera Gaertn. Has effects in stopping leukorrhagia, invigorating spleen, relieving diarrhea, invigorating kidney, and nourishing heart. It can be used for treating chronic diarrhea due to spleen deficiency, anorexia, kidney qi deficiency, nocturnal emission and spermatorrhea due to insecurity of essence closure, whitish and turbid urine due to imbalance between heart and kidney, nocturnal emission and spermatorrhea, spleen deficiency, leukorrhagia due to downward flow of water-dampness, imbalance between heart and kidney, vexation, and insomnia. Ganoderma lucidum, also known as Rue Cao, Ling Zhong Ling, Qin Zhen, is a dried fruiting body of Ganoderma lucidum (Leys. ex Fr.) Karst or Ganoderma sinense ZHao, Xu et Zhang, fungi of Polyporaceae. The shape of the plant is umbrella-shaped, pileus is kidney-shaped, semicircular or nearly circular, and the plant generally grows in mountain forests with high humidity and dim light and mainly grows at the roots of rotten trees or trees. The ganoderma lucidum is a sweet, mild and nontoxic medicinal material, enters lung channels, heart channels, spleen channels and kidney channels, benefits heart-qi, calms the heart, strengthens spleen and stomach, has good effect of treating consumptive disease, and treats various consumptive diseases, such as palpitation, insomnia, dizziness, mental fatigue and hypodynamia. Ganoderma can also be used for treating chronic cough and asthma, and has effects on coronary heart disease, silicosis, and tumor. The ganoderma lucidum soaking water has good water-soluble substances dissolved in the water, has the functions of regulating immunity, resisting radiation, resisting inflammation, resisting oxidation, delaying senescence and protecting liver. Fructus Jujubae is dried mature fruit of Ziziphus jujuba Mill of Rhamnaceae. Has effects of invigorating spleen, replenishing qi, nourishing blood, and tranquilizing. It is indicated for spleen deficiency syndrome, irritability and insomnia.
From the physiological perspective of traditional Chinese medicine, the intestinal tract belongs to the broad range of the spleen and stomach in the theory system of visceral manifestation in traditional Chinese medicine, and the < Ling Shu & Ben Shu > is recorded in the following: the large intestine and the small intestine all belong to the stomach. The spleen and stomach visceral manifestation system of traditional Chinese medicine mainly transports and transforms food, generates qi and blood, and distributes to the five zang organs, so that the five zang organs and six fu organs are full of qi and the physiological functions can be normally exerted. The intestinal flora plays an important role in the process of digestion, absorption and metabolism of the body and supply of nutrient substances, and has the wonderful effect of being different from and working with the spleen function in traditional Chinese medicine. Meanwhile, the feed additive has good digestion, absorption and metabolism, and sufficient nutrient supply, can improve the sub-health state of modern people, and improve the immunity. The bifidobacteria and the lactobacilli are main beneficial bacteria in the intestinal tract and play an important role in the health of the intestinal tract. At present, intestinal beneficial bacteria play a vital role in intestinal health, but how to directionally proliferate is less in domestic research.
Disclosure of Invention
Based on the above, the invention aims to overcome the defects of the prior art and provide the traditional Chinese medicine composition for promoting the proliferation of beneficial bacteria in the intestinal tract and the preparation method and application thereof. The traditional Chinese medicine composition has wide raw material sources, the natural plant raw materials are used as components, the prepared product has the advantages of strong stability, easy storage, low price and the like, and the prepared product has excellent effect on the proliferation of beneficial bacteria in intestinal tracts.
In order to realize the purpose, the technical scheme adopted by the invention is as follows: a traditional Chinese medicine composition for promoting the proliferation of beneficial intestinal bacteria comprises the following raw materials in parts by weight: 5-20 parts of coix seed, 1-15 parts of lotus seed, 1-10 parts of lucid ganoderma and 1-10 parts of Chinese date.
The invention selects the medicinal and edible raw materials with good proliferation effect on beneficial bacteria in intestinal tracts (such as bifidobacterium and lactobacillus), and determines the mixture ratio of the raw materials through a large number of experimental screens, thereby optimizing the optimal combination ratio with remarkable proliferation effect to obtain the traditional Chinese medicine composition. The inventor of the application conducts a large number of experiments when screening medicinal and edible raw materials with good proliferation effect on beneficial bacteria in intestinal tracts (such as bifidobacterium and lactobacillus), few researches in the field at home and abroad are conducted before, and finally, the inventor discovers that the raw materials of coix seeds, lotus seeds, lucid ganoderma and Chinese dates have a very remarkable effect on the proliferation of the beneficial bacteria in the intestinal tracts in a synergistic manner.
Preferably, the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 8-16 parts of coix seeds, 5-10 parts of lotus seeds, 3-6 parts of lucid ganoderma and 3-5 parts of Chinese dates.
The inventor finds that when the proportion of the raw materials of the traditional Chinese medicine composition of coix seed, lotus seed, lucid ganoderma and Chinese date is changed, the effect of proliferating beneficial bacteria in intestinal tracts is directly influenced. The influence of the content of the single raw material on the effect of the traditional Chinese medicine composition is not linear, and the traditional Chinese medicine composition has better effect on improving the intestinal health and better effect on promoting the proliferation of beneficial bacteria in the intestinal tract when the proportion is adopted.
Further preferably, the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 11 parts of coix seeds, 7 parts of lotus seeds, 4 parts of lucid ganoderma and 3 parts of Chinese dates.
After a large number of experiments, the inventor discovers that when 11 parts of coix seeds, 7 parts of lotus seeds, 4 parts of lucid ganoderma and 3 parts of Chinese dates are used, the effect of improving the health of intestinal tracts is optimal, and the effect of promoting the proliferation of beneficial bacteria in the intestinal tracts is better.
In addition, another object of the present invention is to provide a method for preparing the Chinese medicinal composition, comprising the steps of: mixing the coix seed, the lotus seed, the lucid ganoderma and the Chinese date according to a ratio, and extracting to obtain the traditional Chinese medicine composition.
Further, the invention provides another preparation method of the traditional Chinese medicine composition, which comprises the following steps: preparing extracts of coix seed, lotus seed, lucid ganoderma and Chinese date, and mixing the extracts of coix seed, lotus seed, lucid ganoderma and Chinese date according to a proportion to obtain the traditional Chinese medicine composition.
Preferably, the preparation method of the coix seed extract comprises the following steps: extracting, concentrating and drying coix seeds to obtain coix seed extracts; the preparation method of the lotus seed extract comprises the following steps: extracting semen Nelumbinis, concentrating, and drying to obtain semen Nelumbinis extract; the preparation method of the ganoderma lucidum extract comprises the following steps: extracting Ganoderma, concentrating, and drying to obtain Ganoderma extract; the preparation method of the Chinese date extract comprises the following steps: the jujube extract is obtained by extracting, concentrating, alcohol precipitating, dissolving precipitate and drying the jujube.
Further preferably, the preparation method of the coix seed extract comprises the following steps: adding 6-8 times of water to Coicis semen, and extracting at 70-90 deg.C for 2 times, each for 2-2.5 hr; the drying is spray drying, the air inlet temperature of the spray drying is 160-195 ℃, and the air outlet temperature of the spray drying is 60-90 ℃; the preparation method of the lotus seed extract comprises the following steps: adding water 4-5 times the weight of semen Nelumbinis into semen Nelumbinis, and extracting at 70-90 deg.C for 2 times, each for 1.5-2 hr; the drying is spray drying, the air inlet temperature of the spray drying is 160-195 ℃, and the air outlet temperature of the spray drying is 60-90 ℃; the preparation method of the ganoderma lucidum extract comprises the following steps: adding water 8-10 times the weight of Ganoderma into Ganoderma, and extracting at 80-100 deg.C for 1.5-2 hr for 2 times; the drying is vacuum drying, and the temperature of the vacuum drying is 70-85 ℃; the preparation method of the Chinese date extract comprises the following steps: adding water 8-10 times of fructus Jujubae weight into fructus Jujubae, and extracting at 80-100 deg.C for 3 times, each time for 1.5-2 hr; the drying is spray drying, the air inlet temperature of the spray drying is 160-195 ℃, and the air outlet temperature of the spray drying is 60-90 ℃.
In addition, it should be particularly noted that the preparation method of the traditional Chinese medicine composition of the present invention is not limited to the above preparation method, and those skilled in the art can obtain the traditional Chinese medicine composition according to actual situations.
The inventor provides two preparation methods of the traditional Chinese medicine composition, and the traditional Chinese medicine composition is prepared by preparing corresponding extracts from coix seeds, lotus seeds, lucid ganoderma and Chinese dates. The inventor finds that the traditional Chinese medicine composition capable of promoting the proliferation of beneficial intestinal bacteria can be obtained well in the two extraction modes in the practical experiment process, the coix seed extract, the lotus seed extract, the lucid ganoderma extract and the Chinese date extract are prepared, and the coix seed extract, the lotus seed extract, the lucid ganoderma extract and the Chinese date extract are combined according to different proportions, so that the finally obtained traditional Chinese medicine composition has a better effect of promoting the proliferation of beneficial intestinal bacteria.
The invention also aims to provide application of the traditional Chinese medicine composition in preparing food or medicines for improving intestinal health.
Furthermore, the invention provides application of the traditional Chinese medicine composition in preparing food or medicines for promoting the proliferation of beneficial intestinal bacteria.
Preferably, the intestinal beneficial bacteria are at least one of lactobacillus rhamnosus, bifidobacterium animalis, bifidobacterium infantis and lactobacillus helveticus.
In addition, the invention provides application of the traditional Chinese medicine composition in preparing food or medicines for tonifying spleen and nourishing stomach.
In addition, the invention provides a food or a medicament, and the food or the medicament comprises the traditional Chinese medicine composition.
Preferably, the food or drug further comprises an adjuvant. More preferably, the auxiliary material is at least one of a flavoring agent, a thickening agent, a disintegrating agent and a filling agent.
In addition, the invention provides the food or medicine preparation, and the food or medicine preparation is one of granules, capsules, tablets, powder, oral liquid, paste, powder and beverage.
Compared with the prior art, the invention has the beneficial effects that: the invention provides a traditional Chinese medicine composition which has wide raw material sources, and the prepared product has the advantages of strong stability, easy storage, low price and the like. The invention also aims to provide a preparation method and application of the traditional Chinese medicine composition, the product can be further processed into food or medicine, and has good effects on the aspects of improving intestinal health, tonifying spleen and nourishing stomach and promoting the proliferation of beneficial bacteria in intestinal tract, and the product provided by the invention has strong market competitiveness.
Detailed Description
To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to specific examples. In the examples, the experimental methods used were all conventional methods unless otherwise specified, and the materials, reagents and the like used were commercially available without otherwise specified.
The parts of the raw materials in the examples refer to the parts by weight of the raw materials.
The materials used in the examples and comparative examples are now described below, but are not limited to these materials:
the coix seed, the lotus seed, the lucid ganoderma, the Chinese date, the yam and the dried longan pulp are purchased from Guangdong Qingyun yam industry Co., Ltd;
the specific strains for the proliferation test of the invention are as follows:
the strain name is as follows: bifidobacterium animalis (Bifidobacterium animalis), strain number: CICC 6165, strain batch: 20202026, supplier: china center for the preservation and management of industrial microbial strains;
the strain name is as follows: bifidobacterium infantis (Bifidobacterium infantis), strain number: CICC 6069, strain batch: 20172015, supplier: china center for the preservation and management of industrial microbial strains;
the strain name is as follows: lactobacillus rhamnosus (Lactobacillus rhamnosus), strain number: CICC 6001, strain batch: 20172021, supplier: china center for the preservation and management of industrial microbial strains;
the strain name is as follows: lactobacillus helveticus (Lactobacillus helveticus), strain number: r0052, strain batch: HB0069, supplier: raman, canada;
examples 1 to 9 and comparative examples 1 to 19
Example 1
The traditional Chinese medicine composition provided by the embodiment of the invention comprises the following raw materials: 11 parts of coix seeds, 7 parts of lotus seeds, 4 parts of lucid ganoderma and 3 parts of Chinese dates;
the preparation method of the traditional Chinese medicine composition of embodiment 1 comprises the following steps: preparing extracts of coix seed, lotus seed, lucid ganoderma and Chinese date, and mixing the extracts of coix seed, lotus seed, lucid ganoderma and Chinese date according to a proportion to obtain the traditional Chinese medicine composition;
the preparation method of the coix seed extract comprises the following steps: extracting, concentrating and drying coix seeds to obtain coix seed extracts; the specific preparation method of the coix seed extract comprises the following steps: extracting with 8 times of water at 80 deg.C for 2h, extracting with 6 times of water at 80 deg.C for 2h, and spray drying at air inlet temperature of 175 deg.C and air outlet temperature of 75 deg.C; the preparation method of the lotus seed extract comprises the following steps: extracting semen Nelumbinis, concentrating, and drying to obtain semen Nelumbinis extract; the specific preparation method for extracting lotus seeds comprises the following steps: extracting with 5 times of water at 80 deg.C for 1.5 hr, extracting with 4 times of water at 80 deg.C for 1.5 hr, and spray drying at air inlet temperature of 175 deg.C and air outlet temperature of 75 deg.C; the preparation method of the ganoderma lucidum extract comprises the following steps: extracting Ganoderma, concentrating, and drying to obtain Ganoderma extract; the specific preparation method of the ganoderma lucidum extract comprises the following steps: extracting with 10 times of water at 100 deg.C for 1.5h, extracting with 8 times of water at 100 deg.C for 1.5h, and vacuum drying at 80 deg.C; the preparation method of the Chinese date extract comprises the following steps: extracting fructus Jujubae, concentrating, precipitating with ethanol, dissolving precipitate, and drying to obtain fructus Jujubae extract; the preparation method for extracting the Chinese date comprises the following steps: extracting with 10 times of water at 100 deg.C for 1.5 hr, extracting with 8 times of water at 100 deg.C for 1.5 hr, and extracting with 8 times of water at 100 deg.C for 1.5 hr, wherein the air inlet temperature is 175 deg.C and the air outlet temperature is 75 deg.C.
Example 2
The difference between the embodiment and the embodiment 1 is only that the traditional Chinese medicine composition comprises the following raw materials: 5 parts of coix seeds, 15 parts of lotus seeds, 6 parts of lucid ganoderma and 3 parts of Chinese dates.
Example 3
The difference between the embodiment and the embodiment 1 is only that the traditional Chinese medicine composition comprises the following raw materials: 8 parts of coix seeds, 1 part of lotus seeds, 10 parts of lucid ganoderma and 5 parts of Chinese dates.
Example 4
The difference between the embodiment and the embodiment 1 is only that the traditional Chinese medicine composition comprises the following raw materials: 16 parts of coix seeds, 5 parts of lotus seeds, 1 part of lucid ganoderma and 10 parts of Chinese dates.
Example 5
The difference between the embodiment and the embodiment 1 is only that the traditional Chinese medicine composition comprises the following raw materials: 20 parts of coix seeds, 10 parts of lotus seeds, 3 parts of lucid ganoderma and 1 part of Chinese dates.
Example 6
The difference between the embodiment and the embodiment 1 is only that the traditional Chinese medicine composition comprises the following raw materials: 8 parts of coix seeds, 6 parts of lotus seeds, 5 parts of lucid ganoderma and 3 parts of Chinese dates.
Example 7
The difference between the embodiment and the embodiment 1 is only that the traditional Chinese medicine composition comprises the following raw materials: 10 parts of coix seed, 10 parts of lotus seed, 3 parts of lucid ganoderma and 3 parts of Chinese date.
Example 8
The difference between this embodiment and embodiment 1 is only that the preparation method of the traditional Chinese medicine composition is different, and the specific preparation method is as follows: mixing the coix seed, the lotus seed, the lucid ganoderma and the Chinese date according to a ratio, and extracting to obtain the traditional Chinese medicine composition.
Wherein the preparation method comprises the following steps: mixing Coicis semen, semen Nelumbinis, Ganoderma, and fructus Jujubae at a certain proportion, extracting for 2 times, extracting for 2h at 70 deg.C with 10 times of water for the first time, extracting for 2h at 70 deg.C with 8 times of water for the second time, and spray drying, wherein the air inlet temperature is 170 deg.C, and the air outlet temperature is 70 deg.C;
example 9
The difference between this embodiment and embodiment 1 is only that the preparation method of the Chinese medicinal composition is different, and the specific preparation method is as follows:
the method for preparing the traditional Chinese medicine composition of embodiment 9 comprises the following steps: preparing extracts of coix seed, lotus seed, lucid ganoderma and Chinese date, and mixing the extracts of coix seed, lotus seed, lucid ganoderma and Chinese date according to a proportion to obtain the traditional Chinese medicine composition;
the preparation method of the coix seed extract comprises the following steps: extracting, concentrating and drying coix seeds to obtain coix seed extracts; the specific preparation method of the coix seed extract comprises the following steps: extracting with 8 times of water at 70 deg.C for 2 hr, extracting with 6 times of water at 90 deg.C for 2.5 hr, and spray drying at inlet air temperature of 160 deg.C and outlet air temperature of 60 deg.C; the preparation method of the lotus seed extract comprises the following steps: extracting semen Nelumbinis, concentrating, and drying to obtain semen Nelumbinis extract; the specific preparation method for extracting lotus seeds comprises the following steps: extracting with 5 times of water at 70 deg.C for 1.5 hr, extracting with 4 times of water at 90 deg.C for 2 hr, and spray drying at inlet air temperature of 160 deg.C and outlet air temperature of 60 deg.C; the preparation method of the ganoderma lucidum extract comprises the following steps: extracting Ganoderma, concentrating, and drying to obtain Ganoderma extract; the specific preparation method of the ganoderma lucidum extract comprises the following steps: extracting with 10 times of water at 80 deg.C for 1.5h, extracting with 8 times of water at 100 deg.C for 2h, and vacuum drying at 70 deg.C; the preparation method of the Chinese date extract comprises the following steps: extracting fructus Jujubae, concentrating, precipitating with ethanol, dissolving precipitate, and drying to obtain fructus Jujubae extract; the preparation method for extracting the Chinese date comprises the following steps: extracting with 10 times of water at 80 deg.C for 2 hr, extracting with 8 times of water at 90 deg.C for 1.5 hr, extracting with 8 times of water at 100 deg.C for 2 hr, and spray drying at inlet air temperature of 195 deg.C and outlet air temperature of 90 deg.C.
The raw materials and the parts by weight of the concrete comparative examples 1 to 19 are selected as follows:
comparative example 1
The difference between the comparative example and the example 1 is that the traditional Chinese medicine composition comprises the following raw materials: 22 parts of coix seeds, 2 parts of lotus seeds, 0.5 part of lucid ganoderma and 1 part of Chinese date.
Comparative example 2
The difference between the comparative example and the example 1 is that the traditional Chinese medicine composition comprises the following raw materials: 10 parts of coix seeds, 0.5 part of lotus seeds, 2 parts of lucid ganoderma and 1 part of Chinese dates.
Comparative example 3
The difference between the comparative example and the example 1 is that the traditional Chinese medicine composition comprises the following raw materials: 11 parts of coix seeds.
Comparative example 4
The difference between the comparative example and the example 1 is that the traditional Chinese medicine composition comprises the following raw materials: and 7 parts of lotus seeds.
Comparative example 5
The difference between the comparative example and the example 1 is that the traditional Chinese medicine composition comprises the following raw materials: 4 parts of lucid ganoderma.
Comparative example 6
The difference between the comparative example and the example 1 is that the traditional Chinese medicine composition comprises the following raw materials: 3 parts of Chinese dates.
Comparative example 7
The difference between the comparative example and the example 1 is that the traditional Chinese medicine composition comprises the following raw materials: 11 parts of coix seeds and 7 parts of lotus seeds.
Comparative example 8
The difference between the comparative example and the example 1 is that the traditional Chinese medicine composition comprises the following raw materials: 11 parts of coix seeds and 4 parts of lucid ganoderma.
Comparative example 9
The difference between the comparative example and the example 1 is that the traditional Chinese medicine composition comprises the following raw materials: 11 parts of coix seeds and 3 parts of Chinese dates.
Comparative example 10
The difference between the comparative example and the example 1 is that the traditional Chinese medicine composition comprises the following raw materials: 7 parts of lotus seed and 4 parts of lucid ganoderma.
Comparative example 11
The difference between the comparative example and the example 1 is that the traditional Chinese medicine composition comprises the following raw materials: 7 parts of lotus seed and 3 parts of Chinese date.
Comparative example 12
The difference between the comparative example and the example 1 is that the traditional Chinese medicine composition comprises the following raw materials: 4 parts of lucid ganoderma and 3 parts of Chinese date.
Comparative example 13
The difference between the comparative example and the example 1 is that the traditional Chinese medicine composition comprises the following raw materials: 11 parts of coix seeds, 7 parts of lotus seeds and 4 parts of lucid ganoderma.
Comparative example 14
The difference between the comparative example and the example 1 is that the traditional Chinese medicine composition comprises the following raw materials: 11 parts of coix seeds, 7 parts of lotus seeds and 3 parts of Chinese dates.
Comparative example 15
The difference between the comparative example and the example 1 is that the traditional Chinese medicine composition comprises the following raw materials: 7 parts of lotus seed, 4 parts of lucid ganoderma and 3 parts of Chinese date.
Comparative example 16
The difference between the comparative example and the example 1 is that the traditional Chinese medicine composition comprises the following raw materials: 11 parts of coix seeds, 4 parts of lucid ganoderma and 3 parts of Chinese dates.
Comparative example 17
The difference between the comparative example and the example 1 is that the traditional Chinese medicine composition comprises the following raw materials: 11 parts of Chinese yam, 7 parts of lotus seed, 4 parts of lucid ganoderma and 3 parts of Chinese date.
Comparative example 18
The difference between the comparative example and the example 1 is that the traditional Chinese medicine composition comprises the following raw materials: 11 parts of coix seeds, 7 parts of lotus seeds, 4 parts of lucid ganoderma and 3 parts of longan aril.
Comparative example 19
The difference between the comparative example and the example 1 is that the traditional Chinese medicine composition comprises the following raw materials: 3 parts of coix seeds, 17 parts of lotus seeds, 4.5 parts of lucid ganoderma and 0.5 part of Chinese date.
Application example 1
A hard capsule comprises the Chinese medicinal composition prepared in example 1 and 0.5 part of magnesium stearate.
Application example 2
A granule comprises the Chinese medicinal composition prepared in example 4, 32 parts of maltodextrin, 1 part of mogroside and 34 parts of isomalt.
Effect verification
Test example 1
The test process comprises the following steps:
(1) preparing the traditional Chinese medicine compositions of the examples and the comparative examples into 0.01g/ml samples for later use, and preparing the samples by adopting sterile distilled water;
(2) carrying out beneficial bacterium proliferation test; blank group: 10ml of MRS liquid culture medium without any raw materials and probiotics; negative control group: under aseptic operation conditions, taking 8ml of MRS liquid culture medium, and adding 1ml of bacterial liquid (bifidobacterium animalis 6165) and 1ml of aseptic distilled water; experimental groups: under the aseptic operation condition, 8ml of MRS liquid culture medium is respectively added with 1ml of bacterial liquid, the concentration of the bacterial liquid is 10^6cfu/ml (bifidobacterium animalis 6165) and 1ml of sample solution; anaerobic culture is carried out for 24h at 36 +/-1 ℃, and an ultraviolet spectrophotometer is used for detecting OD of a sample 600 And (3) light absorption values are obtained in each experiment in parallel, and the average value of the measurement is taken as a final result.
The results of the test on the proliferation of bifidobacterium animalis 6165 are shown in table 1:
TABLE 1
Note: p < 0.05 compared to negative control;
from the above table, it can be seen that: compared with the negative control, the examples 1 to 9 and the comparative examples 1 to 19 can promote the proliferation of the bifidobacterium animalis 6165 to different degrees (P is less than 0.05), the growth of the bifidobacterium animalis 6165 is more favorable than the pure culture of the culture medium, and the proliferation effect of the examples is stronger than that of the comparative examples.
The experimental results in the table show that the traditional Chinese medicine composition prepared from coix seeds, lotus seeds, lucid ganoderma and Chinese dates according to specific weight parts has an obvious proliferation effect on bifidobacterium animalis 6165. When 8-16 parts of coix seeds, 5-10 parts of lotus seeds, 3-6 parts of lucid ganoderma and 3-5 parts of Chinese dates are used, the effect of promoting the proliferation of beneficial bacteria (bifidobacterium animalis 6165) in the intestinal tract is better; wherein, when 11 parts of coix seeds, 7 parts of lotus seeds, 4 parts of lucid ganoderma and 3 parts of Chinese dates, the proliferation effect on animal bifidobacterium 6165 is strongest (P is less than 0.05), which indicates that the embodiment 1 is the best combination.
The traditional Chinese medicine composition comprises three components in the traditional Chinese medicine composition in the comparative examples 3-6, two components in the traditional Chinese medicine composition in the comparative examples 7-12, one component in the traditional Chinese medicine composition in the comparative examples 13-16, arillus longan or Chinese yam in the comparative examples 17-18 to replace one component in the traditional Chinese medicine composition, the total amount of active ingredients is kept unchanged, the comparative examples 1-2 and the comparative example 19 comprise all the components in the traditional Chinese medicine composition, and the proportion is not within the protection range of the invention.
Test example 2
The test process comprises the following steps:
(1) preparing the traditional Chinese medicine compositions of the examples and the comparative examples into 0.01g/ml samples for later use, and preparing the samples by adopting sterile distilled water;
(2) carrying out beneficial bacterium proliferation test; blank group: 10ml of MRS liquid culture medium without any raw materials and probiotics; negative control group: taking 8ml of MRS liquid culture under the aseptic operation conditionAdding 1ml of bacterial liquid into nutrient medium, wherein the concentration of the bacterial liquid is 10^6cfu/ml (bifidobacterium infantis) and 1ml of sterile distilled water; experimental groups: under the aseptic operation condition, taking 8ml of MRS liquid culture medium, and respectively adding 1ml of bacterial liquid (bifidobacterium infantis) and 1ml of sample solution; anaerobic culture is carried out for 24h at 36 +/-1 ℃, and an ultraviolet spectrophotometer is used for detecting OD of a sample 600 And (3) light absorption values are obtained in each experiment in parallel, and the average value of the measurement is taken as a final result.
The results of the test on the proliferation of [ bifidobacterium infantis ] are shown in table 2:
TABLE 2
Note: p < 0.05 compared to negative control;
from the above table, it can be seen that: compared with the negative control, the proliferation of the bifidobacterium infantis (P is less than 0.05) is promoted to different degrees in the example groups 1 to 9 and the comparative example groups 1 to 19, the growth of the bifidobacterium infantis is more favorable than that of the bifidobacterium infantis when the culture medium is simply cultured, and the proliferation effect of the example groups is stronger than that of the comparative example groups.
The experimental results in the table show that the traditional Chinese medicine composition prepared from coix seeds, lotus seeds, lucid ganoderma and Chinese dates in specific weight parts has an obvious proliferation effect on the bifidobacterium infantis. When 8-16 parts of coix seeds, 5-10 parts of lotus seeds, 3-6 parts of lucid ganoderma and 3-5 parts of Chinese dates are used, the effect of promoting the proliferation of beneficial bacteria (bifidobacterium infantis) in the intestinal tract is better; wherein, when 11 parts of coix seeds, 7 parts of lotus seeds, 4 parts of lucid ganoderma and 3 parts of Chinese dates, the proliferation effect on the bifidobacterium infantis is strongest (P is less than 0.05), which indicates that the embodiment 1 is the best combination.
The traditional Chinese medicine composition comprises three components in the traditional Chinese medicine composition in the comparative examples 3-6, two components in the traditional Chinese medicine composition in the comparative examples 7-12, one component in the traditional Chinese medicine composition in the comparative examples 13-16, arillus longan or Chinese yam in the comparative examples 17-18 to replace one component in the traditional Chinese medicine composition, the total amount of active ingredients is kept unchanged, the comparative examples 1-2 and the comparative example 19 comprise all the components in the traditional Chinese medicine composition, and the proportion is not within the protection range of the invention.
Test example 3
The test process comprises the following steps:
(1) preparing the traditional Chinese medicine compositions of the examples and the comparative examples into 0.01g/ml samples for later use, and preparing the samples by adopting sterile distilled water;
(2) carrying out beneficial bacterium proliferation test; blank group: 10ml of MRS liquid culture medium without any raw materials and probiotics; negative control group: under the aseptic operation condition, 8ml of MRS liquid culture medium is taken and added with 1ml of bacterial liquid, the concentration of the bacterial liquid is 10^6cfu/ml (Lactobacillus rhamnosus 6001) and 1ml of aseptic distilled water; experimental groups: under the aseptic operation condition, taking 8ml of MRS liquid culture medium, and respectively adding 1ml of bacterial liquid (lactobacillus rhamnosus 6001) and 1ml of sample solution; anaerobic culture is carried out for 24h at 36 +/-1 ℃, and an ultraviolet spectrophotometer is used for detecting OD of a sample 600 And (3) light absorption values are obtained in each experiment in parallel, and the average value of the measurement is taken as a final result.
The results of the test on the proliferation of lactobacillus rhamnosus 6001 are shown in table 3:
TABLE 3
Note: p < 0.05 compared to negative control;
from the above table, it can be seen that: compared with a negative control, the proliferation of the lactobacillus rhamnosus 6001 can be promoted to different degrees (P is less than 0.05) in each of the example groups 1-9 and the comparative example groups 1-19, the growth of the lactobacillus rhamnosus 6001 is more favorable than that of the lactobacillus rhamnosus 6001 cultured by a culture medium alone, and the proliferation effect of the example groups is stronger than that of the comparative example groups.
The experimental results in the table show that the traditional Chinese medicine composition prepared from coix seeds, lotus seeds, lucid ganoderma and Chinese dates in a specific weight part selection has an obvious proliferation effect on lactobacillus rhamnosus 6001. When the coix seed is 8-16 parts, the lotus seed is 5-10 parts, the ganoderma lucidum is 3-6 parts and the jujube is 3-5 parts, the effect of promoting the proliferation of the beneficial intestinal bacteria (lactobacillus rhamnosus 6001) is better, wherein when the coix seed is 11 parts, the lotus seed is 7 parts, the ganoderma lucidum is 4 parts and the jujube is 3 parts, the proliferation effect on the lactobacillus rhamnosus 6001 is strongest (P is less than 0.05), and the embodiment 1 is the best combination and collocation.
The traditional Chinese medicine composition comprises three components in the traditional Chinese medicine composition in the comparative examples 3-6, two components in the traditional Chinese medicine composition in the comparative examples 7-12, one component in the traditional Chinese medicine composition in the comparative examples 13-16, arillus longan or Chinese yam in the comparative examples 17-18 to replace one component in the traditional Chinese medicine composition, the total amount of active ingredients is kept unchanged, the comparative examples 1-2 and the comparative example 19 comprise all the components in the traditional Chinese medicine composition, and the proportion is not within the protection range of the invention.
Test example 4
The test process comprises the following steps:
(1) preparing the traditional Chinese medicine compositions of the examples and the comparative examples into 0.01g/ml samples for later use, and preparing the samples by adopting sterile distilled water;
(2) carrying out beneficial bacterium proliferation test; blank group: 10ml of MRS liquid culture medium without any raw materials and probiotics;negative control group: under the aseptic operation condition, 8ml of MRS liquid culture medium is taken and added with 1ml of bacterial liquid, the concentration of the bacterial liquid is 10^6cfu/ml (Lactobacillus helveticus) and 1ml of aseptic distilled water; experimental groups: under the aseptic operation condition, taking 8ml of MRS liquid culture medium, and respectively adding 1ml of bacterial liquid (Lactobacillus helveticus) and 1ml of sample solution; anaerobic culture is carried out for 24h at 36 +/-1 ℃, and an ultraviolet spectrophotometer is used for detecting OD of a sample 600 And (3) light absorption values are obtained in each experiment in parallel, and the average value of the measurement is taken as a final result.
The results of the test on proliferation of [ lactobacillus helveticus ] are shown in table 4:
TABLE 4
Note: p < 0.05 compared to negative control;
from the above table, it can be seen that: compared with the negative control, the proliferation of the Lactobacillus helveticus is promoted to different degrees (P is less than 0.05) in the example groups 1 to 9 and the comparative example groups 1 to 19, the growth of the Lactobacillus helveticus is more favorable than that of the Lactobacillus helveticus cultured by a culture medium alone, and the proliferation effect of the example groups is stronger than that of the comparative example groups.
The experimental results in the table show that the traditional Chinese medicine composition prepared from coix seeds, lotus seeds, lucid ganoderma and Chinese dates in specific weight parts has an obvious proliferation effect on lactobacillus helveticus. When 8-16 parts of coix seeds, 5-10 parts of lotus seeds, 3-6 parts of lucid ganoderma and 3-5 parts of Chinese dates are used, the effect of promoting the proliferation of intestinal beneficial bacteria (lactobacillus helveticus) is better; wherein, when 11 parts of coix seeds, 7 parts of lotus seeds, 4 parts of lucid ganoderma and 3 parts of Chinese dates, the proliferation effect on lactobacillus helveticus is strongest (P is less than 0.05), which indicates that the embodiment 1 is the best combination.
The traditional Chinese medicine composition comprises three components in the traditional Chinese medicine composition in the comparative examples 3-6, two components in the traditional Chinese medicine composition in the comparative examples 7-12, one component in the traditional Chinese medicine composition in the comparative examples 13-16, arillus longan or Chinese yam in the comparative examples 17-18 to replace one component in the traditional Chinese medicine composition, the total amount of active ingredients is kept unchanged, the comparative examples 1-2 and the comparative example 19 comprise all the components in the traditional Chinese medicine composition, and the proportion is not within the protection range of the invention.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (15)
1. The traditional Chinese medicine composition for promoting the proliferation of beneficial intestinal bacteria is characterized by comprising the following raw materials in parts by weight: 5-20 parts of coix seed, 1-15 parts of lotus seed, 1-10 parts of lucid ganoderma and 1-10 parts of Chinese date.
2. The traditional Chinese medicine composition of claim 1, which comprises the following raw materials in parts by weight: 8-16 parts of coix seeds, 5-10 parts of lotus seeds, 3-6 parts of lucid ganoderma and 3-5 parts of Chinese dates.
3. The traditional Chinese medicine composition of claim 1, which comprises the following raw materials in parts by weight: 11 parts of coix seeds, 7 parts of lotus seeds, 4 parts of lucid ganoderma and 3 parts of Chinese dates.
4. A method for preparing a Chinese medicinal composition as claimed in any one of claims 1 to 3, comprising the steps of: mixing the coix seed, the lotus seed, the lucid ganoderma and the Chinese date according to a ratio, and extracting to obtain the traditional Chinese medicine composition.
5. A method for preparing a Chinese medicinal composition as claimed in any one of claims 1 to 3, comprising the steps of: preparing extracts of coix seed, lotus seed, lucid ganoderma and Chinese date, and mixing the extracts of coix seed, lotus seed, lucid ganoderma and Chinese date according to a proportion to obtain the traditional Chinese medicine composition.
6. The method for preparing a Chinese medicinal composition according to claim 5, wherein the coix seed extract is prepared by the following steps: extracting, concentrating and drying coix seeds to obtain coix seed extracts; the preparation method of the lotus seed extract comprises the following steps: extracting semen Nelumbinis, concentrating, and drying to obtain semen Nelumbinis extract; the preparation method of the ganoderma lucidum extract comprises the following steps: extracting Ganoderma, concentrating, and drying to obtain Ganoderma extract; the preparation method of the Chinese date extract comprises the following steps: the jujube extract is obtained by extracting, concentrating, precipitating with ethanol, dissolving the precipitate and drying the jujube.
7. The method for preparing a Chinese medicinal composition for promoting the proliferation of beneficial intestinal bacteria according to claim 6, wherein the coix seed extract is prepared by the following steps: adding 6-8 times of water to Coicis semen, and extracting at 70-90 deg.C for 2 times, each for 2-2.5 hr; the drying is spray drying, the air inlet temperature of the spray drying is 160-195 ℃, and the air outlet temperature of the spray drying is 60-90 ℃; the preparation method of the lotus seed extract comprises the following steps: adding water 4-5 times the weight of semen Nelumbinis into semen Nelumbinis, and extracting at 70-90 deg.C for 2 times, each for 1.5-2 hr; the drying is spray drying, the air inlet temperature of the spray drying is 160-195 ℃, and the air outlet temperature of the spray drying is 60-90 ℃; the preparation method of the ganoderma lucidum extract comprises the following steps: adding water 8-10 times the weight of Ganoderma into Ganoderma, and extracting at 80-100 deg.C for 2 times, each for 1.5-2 hr; the drying is vacuum drying, and the temperature of the vacuum drying is 70-85 ℃; the preparation method of the Chinese date extract comprises the following steps: adding water 8-10 times of fructus Jujubae weight into fructus Jujubae, and extracting at 80-100 deg.C for 3 times, each time for 1.5-2 hr; the drying is spray drying, the air inlet temperature of the spray drying is 160-195 ℃, and the air outlet temperature of the spray drying is 60-90 ℃.
8. Use of a Chinese medicinal composition as claimed in any one of claims 1 to 3 in the preparation of a food or medicament for improving gut health.
9. Use of the Chinese medicinal composition of any one of claims 1 to 3 in the preparation of a food or a medicament for promoting the proliferation of beneficial intestinal bacteria.
10. The use of claim 9, wherein the beneficial gut bacteria is at least one of lactobacillus rhamnosus, bifidobacterium animalis, bifidobacterium infantis, lactobacillus helveticus.
11. Use of the Chinese medicinal composition of any one of claims 1-3 in the preparation of food or medicament for invigorating spleen and stomach.
12. A food product comprising the Chinese medicinal composition of any one of claims 1-3.
13. The food product of claim 12, wherein the components of the food product further comprise an adjuvant.
14. The food product of claim 13, wherein the excipient is at least one of a flavoring agent, a thickening agent, a disintegrant, and a filler.
15. The food of claim 14, wherein the food is in the form of one of granules, capsules, tablets, powders, oral liquids, pastes, powders, and beverages.
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