CN114947156B

CN114947156B - Preparation device and preparation method of polypeptide biological puffed compound feed

Info

Publication number: CN114947156B
Application number: CN202210913310.7A
Authority: CN
Inventors: 李欢; 马惠文; 李建文; 付勇
Original assignee: Yantai Dale Feed Co ltd
Current assignee: Yantai Dale Feed Co ltd
Priority date: 2022-08-01
Filing date: 2022-08-01
Publication date: 2022-10-25
Anticipated expiration: 2042-08-01
Also published as: CN114947156A; CN115721031A

Abstract

The invention provides a preparation device and a preparation method of a polypeptide biological puffed compound feed. The preparation device comprises a stirring bin, wherein a first discharge hole is formed in the lower end of the stirring bin, and the stirring bin is communicated with the lower part of the middle partition plate through the first discharge hole; the lateral wall of stirring the storehouse is the material that permeates water, hugs closely the top of intermediate bottom and has seted up the liquid outlet on the lateral wall of the jar body. The middle part of jar body upper end is provided with first motor, and the output of first motor is connected with stirring rod and a plurality of telescopic link, and the lower extreme of every telescopic link is fixed with the clamp plate. The device has solved that harmony is poor between the current feed processing equipment, needs carry the material between different equipment, the problem that wastes time and energy.

Description

Preparation device and preparation method of polypeptide biological puffed compound feed

Technical Field

The invention belongs to the technical field of feed processing equipment, and particularly relates to a preparation device and a preparation method of a polypeptide biological puffed compound feed.

Background

The prawn is a group with high yield and high economic value in marine shrimps. In recent years, commercial culture of marine shrimps has been carried out in large quantities, and the yield is rapidly increasing. The artificial compound feed is the material basis for the healthy culture of the prawns.

Feed processing is an important production link in feed production. The existing feed processing equipment mainly comprises equipment for discharging, storing, crushing, mixing, packaging and the like, the coordination between the equipment is poor, materials need to be carried between different equipment, especially when liquid in the feed mixture needs to be extracted, the feed mixture needs to be carried to special extrusion or separation equipment, and the liquid is extracted and then carried to the next link, so that time and labor are wasted. For example, chinese patent cn201520281720.X provides a pneumatic feed processing unit, including mixing machine, rubbing crusher, rotatory unloading valve, roots's fan, dust filter, fall the ware and the material filter of making an uproar, the top of mixing machine is connected rubbing crusher, rubbing crusher connects the feeding storehouse, the below of mixing machine is connected rotatory unloading valve, the material filter on the mixing machine is through connecting air volume regulator, the one end of air volume regulator is through rotatory unloading valve connection material storehouse, the other end of air volume regulator is through dust filter and the roots's fan of making an uproar connection. The processing unit uses a large number of pipelines and transportation equipment, and wastes time and labor.

Disclosure of Invention

The invention aims to solve the problems that the existing feed processing equipment has poor coordination and needs to carry materials among different equipment, and particularly, when liquid in a feed mixture needs to be extracted, the feed mixture needs to be carried to special extrusion or separation equipment and then carried to the next link after the liquid is extracted, so that time and labor are wasted. In order to solve the technical problems, the invention discloses the following technical scheme:

the utility model provides a biological popped formula feed's of polypeptide preparation facilities, includes jar body and popped conveyer, the jar body includes:

the first feeding port is arranged at the upper end of the tank body; the first charging hole is provided with

The charging door can control the opening and closing of the first charging opening;

the middle partition plate is fixed in the middle of the tank body;

the stirring bin is arranged above the middle partition plate; the lower end of the stirring bin is provided with

The first discharge hole is used for communicating the stirring bin with the lower part of the middle partition plate;

the discharge valve is arranged at the first discharge hole;

the grinding bin is arranged below the first discharge hole and is rotatably connected with the side wall of the tank body;

the output end of the second motor is fixedly connected with the grinding bin;

the second discharge hole is formed in the bottom of the tank body;

the lower part of the second discharge hole is communicated with the inlet of the bulking conveyor;

the side wall of the stirring bin is made of a water-permeable material, a gap is reserved between the side wall of the stirring bin and the side wall of the tank body, a liquid outlet is formed in the side wall of the tank body and is close to the upper part of the middle partition plate, and a liquid outlet valve is arranged on the liquid outlet;

the middle part of jar body upper end is provided with first motor, the output of first motor is connected with stirring rod and a plurality of telescopic link, every the lower extreme of telescopic link is fixed with the clamp plate.

Further, the preparation facilities still includes the air-blower, the entry of air-blower with the terminal upper portion intercommunication of popped conveyer, the export respectively with stir the storehouse and grind jar side wall intercommunication between storehouse and the second discharge gate.

Further, an electric heater is arranged at the output end of the air blower.

Furthermore, the number of the first charging openings is two, and the two first charging openings are respectively positioned at two ends of the top of the tank body; the charging door comprises a circular sliding ring, the top of the tank body is provided with a groove matched with the sliding ring, and the sliding ring is in sliding contact with the groove; two ends of the sliding ring are respectively and fixedly connected with two shielding plates.

Further, be provided with the abrasive disc in the grinding storehouse, the abrasive disc below is provided with gravity sensor, when gravity sensor detects weight and reaches the setting value, closes first discharge gate.

Further, a second charging hole is formed between the second discharging hole and the puffing conveyor.

A method for preparing polypeptide biological puffed compound feed by using the device comprises the following steps:

opening a feeding door, adding quantitative fermentation raw materials and liquid strains into the stirring bin from a first feeding port, and closing the feeding door;

starting a first motor to drive a stirring rod to stir, mix and ferment the materials in the stirring bin; after fermentation is completed, opening a feed door, adding enzyme and water, then closing the feed door, starting a first motor to stir again, and carrying out enzymolysis; after enzymolysis is finished, opening a liquid outlet valve, starting each telescopic rod, pushing each pressing plate to extrude the materials in the stirring bin from top to bottom, and enabling part of liquid to penetrate through the side wall of the stirring bin and then flow out of the liquid outlet;

opening a feed door, and adding the ingredients mixed by the primary ingredients; closing the feeding door, and starting the first motor to stir and mix; opening a discharge valve, and allowing part of the polypeptide mixture to fall into a grinding bin from a stirring bin; closing the discharge valve, and carrying out superfine grinding on the materials in the grinding bin; after the grinding is finished, starting a second motor to drive the grinding bin to rotate for 180 degrees, and enabling the ground material to fall into the bottom of the tank body; the bulking conveyor is started, the materials are continuously taken away from the bottom of the tank body, meanwhile, the liquid ingredients mixed by the secondary ingredients are continuously added from the second feeding port, and the bulking conveyor outputs the bulking granules.

Further, the liquid strain comprises the following components: bifidobacterium 108 CFU/g, lactobacillus acidovorus 2X 108 CFU/g, bacillus subtilis 108 CFU/g, bacillus licheniformis 2X 108 CFU/g.

Further, the secondary ingredient mixing comprises: adding phospholipid oil 20-60kg, fish oil 40-80kg, bile acid 1-2kg, glutathione 0.2-0.3kg, and vitamins 30-50kg into each ton of the superfine pulverized mixture.

Has the advantages that:

1. the preparation device integrates mixing, fermentation, enzymolysis, liquid extraction, crushing and puffing granulation, can simultaneously perform multiple operations which are related to each other, reduces the transportation of materials among different devices, has high automation degree and saves manpower.

2. The invention can automatically extract the liquid in the feed mixture without carrying materials, saves time and labor and is thorough in extraction. The extraction amount can be controlled, and the water content of the mixture after the liquid is extracted can be controlled.

3. The preparation device provided by the invention utilizes the redundant heat of the puffing conveyor to provide heat for fermentation, enzymolysis and preheating before puffing, so that the energy efficiency is improved.

4. The optimal strain ratio is selected through experiment comparison by proportioning the common fermentation strains in the four strains, so that more proteins are fermented and converted into peptides.

5. The secondary ingredients are mixed and arranged after the superfine grinding, so that the unstable components are prevented from being damaged by the superfine grinding.

Drawings

FIG. 1 is a schematic diagram of an apparatus for preparing a biological puffed compound polypeptide feed according to an embodiment of the present invention;

FIG. 2 is a schematic structural view of the inside of a can body according to an embodiment of the present invention;

FIG. 3 is a schematic diagram of the stirring rod, the telescopic rod and the pressing plate according to the embodiment of the present invention;

FIG. 4 is a block diagram of a heating system according to an embodiment of the present invention;

FIG. 5 is a schematic diagram of a loading door according to an embodiment of the present invention.

In the figure: 1-tank body, 2-first charging hole, 3-charging door, 301-sliding ring, 302-baffle plate, 4-intermediate partition plate, 5-stirring bin, 6-first discharging hole, 7-discharging valve, 8-grinding bin, 9-second motor, 10-second discharging hole, 11-puffing conveyor, 12-blower, 13-electric heater, 14-liquid outlet, 15-liquid outlet valve, 16-first motor, 17-stirring rod, 18-telescopic rod, 19-pressing plate, 20-grinding disc, 21-second charging hole and 22-three-way regulating valve.

Detailed Description

The present invention will be described in detail and clearly with reference to the following examples.

Example 1

The embodiment provides a biological popped formula feed preparation facilities of polypeptide, including a

jar body

1, 1 upper ends of jar body are provided with first charge door 2, are provided with charge door 3 on the first charge door 2. Illustratively, the first feed ports 2 are two and are respectively positioned at two ends of the top of the tank body 1. The charge door 3 includes circular

shape sliding ring

301, and 1 top of the jar body is provided with the recess with sliding ring 301 complex, sliding ring 301 and recess sliding contact. Two shielding plates 302 are fixedly connected to the sliding ring 301 respectively, and the two shielding plates 302 can cover the first feeding port 2 or open the first feeding port 2 simultaneously by rotating the sliding ring 301. The middle part in the jar body 1 is provided with intermediate bottom 4, and intermediate bottom 4's top is for stirring storehouse 5, stirs and is provided with temperature sensor in the storehouse 5. The side wall of the stirring bin 5 is made of a water-permeable material, and for example, water-permeable concrete can be used. A gap is reserved between the side wall of the stirring bin 5 and the side wall of the tank body 1, a liquid outlet 14 is formed in the side wall of the tank body 1 and close to the upper part of the middle partition plate 4, and a liquid outlet valve 15 is arranged on the liquid outlet 14. A first motor 16 is arranged in the middle of the upper end of the tank body 1, and the output end of the first motor 16 is connected with a stirring rod 17 for stirring the materials in the stirring bin 5. The output end of the first motor 16 is further connected with a plurality of telescopic rods 18, and the lower end of each telescopic rod 18 is fixed with a pressing plate 19. Mix the lower extreme in storehouse 5 and seted up first discharge gate 6, be provided with bleeder valve 7 on the first discharge gate 6. Mix the below in storehouse 5 and be provided with and grind storehouse 8, grind storehouse 8 and jar body 1 lateral wall rotatable coupling, be fixed with second motor 9 on the 1 lateral wall of the jar body, the output of second motor 9 and grind 8 fixed connection in storehouse can control and grind 8 upsets in storehouse. A grinding disc 20 is arranged in the grinding bin 8, and a gravity sensor is arranged below the grinding disc 20 and used for detecting the gravity borne by the grinding disc 20. The polishing disc 20 is conventional and will not be described in detail herein. Further, 8 lateral walls in grinding storehouse are at the inside hem in top, prevent that the grinding in-process material flies out. The bottom of the tank body 1 is of a conical structure, and a second discharge hole 10 is formed in the center of the conical structure.

The lower part of the second discharge port 10 is communicated with the inlet of the bulking conveyor 11. The bulking conveyor 11 is conventional and will not be described in detail herein. A second charging hole 21 is further arranged between the second discharging hole 10 and the bulking conveyor 11, the second charging hole 21 is used for conveying liquid materials, the charging amount is controlled by a flow controller, and the flow controller is in the prior art.

The feed preparation device is also provided with a heating system which comprises an air blower 12, wherein the inlet of the air blower 12 is communicated with the upper part of the tail end of the puffing conveyor 11, and the outlet of the air blower is communicated with the side wall of the tank body 1 between the stirring bin 5 and the grinding bin 8 and the second discharge hole 10 respectively. The blower 12 extracts and conveys the excess heat generated by the bulking conveyor 11 into the tank 1. The output end of the blower 12 is provided with an electric heater 13 for supplementing heat, and the rear end of the electric heater 13 is provided with a three-way regulating valve 22 for regulating the air flow entering the two heating pipelines of the tank body 1.

Example 2

This example provides a method of preparing a polypeptide bio-expanded compound feed using the apparatus of example 1, comprising the steps of:

the feeding door 3 is opened, a certain amount of fermentation raw materials and liquid strains are added into the stirring bin 5 from the first feeding port 2, and the feeding door 3 is closed. Preferably, the liquid seed culture comprises the following components: 108 CFU/g of bifidobacterium, 2 multiplied by 108 CFU/g of dry sour lactobacillus, 108 CFU/g of bacillus subtilis and 2 multiplied by 108 CFU/g of bacillus licheniformis.

The first motor 16 is started to drive the stirring rod 17 to stir and mix the materials in the stirring bin 5. The temperature sensor detects the temperature in the stirring bin 5, and controls the blower 12, the electric heater 13 and the three-way regulating valve 22 to provide heat for the stirring bin 5 and maintain the fermentation temperature. After the fermentation is completed, the feed door 3 is opened, the enzyme and the water are added, then the feed door 3 is closed, the first motor 16 is started to stir again, and the temperature in the stirring bin 5 is controlled in the same way to carry out enzymolysis. After enzymolysis, open out liquid valve 15, start each telescopic link 18, promote each clamp plate 19 and from top to bottom the extrusion stir the material in the storehouse 5 for most liquid sees through stirring the storehouse 5 lateral wall, and then flows out from liquid outlet 14. In order to extract polypeptide liquid as much as possible, after the first extrusion, the pressing plate 19 can be lifted upwards, the stirring rod 17 can properly stir the materials, and then the extracted polypeptide liquid can be extruded again. The extraction amount of the liquid can be controlled by controlling the extrusion force, so that the water content in the mixture after the liquid is extracted is controlled. The polypeptide mixture with low water content left in the stirring bin 5 is used for preparing prawn feed.

The feed gate 3 is opened and the ingredients of the first ingredient mix are added. Preferably, the ingredients of the one-time ingredient mixing include: adding 250-400kg astaxanthin per ton mixture. The loading door 3 is closed and the first motor 16 is started to mix. Open bleeder valve 7, partial polypeptide mixture falls into grinding storehouse 8 from stirring storehouse 5, and when the gravity sensor in the grinding storehouse 8 detected and reached the setting value, closing bleeder valve 7 to the weight of control grinding material. The grinding disk 20 is started to carry out superfine grinding on the materials. After the crushing is finished, the second motor 9 is started to drive the grinding bin 8 to rotate by 180 degrees, and the ground material falls into the bottom of the tank body 1. The bulking conveyor 11 is started, the material is continuously taken away from the bottom of the tank body 1, and simultaneously the liquid ingredient mixed by the secondary ingredient is continuously added through the second feeding port 21. Preferably, the ingredients of the secondary ingredient mixing comprise: adding phospholipid oil 20-60kg, fish oil 40-80kg, bile acid 1-2kg, glutathione 0.2-0.3kg, and vitamins 30-50kg into each ton of the superfine pulverized mixture. And the puffing conveyor 11 outputs the puffed granules, and then is dried and cooled. Furthermore, the dried expanded feed can be subjected to anaerobic fermentation treatment by adopting vacuum spraying and constant-temperature anaerobic fermentation technologies to kill mould and harmful bacteria.

This embodiment fodder preparation facilities stirs storehouse 5, grinds 8 and the popped conveyer 11 and can move simultaneously, and air-blower 12 extracts unnecessary heat from the end of popped conveyer 11 and carries to stirring storehouse 5, for fermentation, enzymolysis provides the heat, can preheat popped material with heat transport to jar 1 bottom region simultaneously to reduce the energy consumption.

Example 3

The embodiment provides detailed preparation steps and specific preparation conditions of the polypeptide biological puffed compound feed:

1. screening and proportioning raw materials: crushing the fish and shrimp raw materials to 40 meshes, and filtering to obtain fish and shrimp pulp; taking 20-40 parts of fish and shrimp pulp and 60-80 parts of peanut meal, and uniformly mixing and stirring to form a raw material mixture.

2. And (3) microbial fermentation:

(1) Screening and identification of functional lactic acid bacteria

Through investigation, four kinds of most used feed fermentation strains are screened out, including: bifidobacterium, lactobacillus acidophilus, bacillus subtilis and bacillus licheniformis.

(2) Comparative experiment of strain formulation

Each group of raw materials has the same components (30 parts of fish and shrimp pulp and 70 parts of peanut meal) and the same total mass of strains, and the fermentation test is carried out by respectively using 11 strain proportions (bifidobacterium: lactobacillus xerophilus: bacillus subtilis: bacillus licheniformis) shown in the table 1 by adopting the same operation method. And after the fermentation end point is reached, detecting indexes: moisture, small peptides, pH, crude protein. Regarding fermentation time and end point: according to the environmental temperature, the completion time of the fermentation of the raw materials is mastered and controlled to be 72-96 h, the optimal fermentation temperature is 34-39 ℃, and the judgment standard of successful fermentation is as follows: has fermented wine acid flavor and pH value of about 5. According to the evaluation of small peptide content and pH, the experimental formula of the 10# strain (bifidobacterium: lactobacillus xerox: bacillus subtilis: bacillus licheniformis = 1.

(Code)	Strain proportioning	Water content%	pH	Small peptide%	Crude protein%
						1#	1:1:1:1	39.72%	5.22	8.74%	26.95%
2#	2:1:1:1	39.53%	5.31	7.88%	27.21%
						3#	1:2:1:1	40.67%	4.97	7.70%	26.73%
4#	1:1:2:1	41.01%	5.02	5.04%	27.56%
						5#	1:1:1:2	41.68%	5.03	7.18%	26.91%
6#	2:2:1:1	40.85%	4.95	7.86%	26.97%
						7#	2:1:2:1	40.97%	5.02	8.62%	26.53%
8#	2:1:1:2	39.93%	5.08	8.45%	26.38%
						9#	1:2:2:1	41.21%	5.15	9.02%	26.30%
10#	1:2:1:2	41.72%	5.08	10.38%	26.18%
						11#	1:1:2:2	41.36%	5.13	9.18%	26.34%

TABLE 1 evaluation of fermentation effect of leavening agents of different formulations on biologically fermented expanded feed

(3) Preparation of liquid strains and fermentation

The content of each strain is as follows: bifidobacterium 108 CFU/g, lactobacillus acidovorus 2X 108 CFU/g, bacillus subtilis 108 CFU/g, bacillus licheniformis 2X 108 CFU/g.

The raw material mixture and the liquid strain are mixed according to the proportion of 1:0.3, forming a microorganism-raw material mixture, fermenting at 34-39 ℃, and obtaining a fermentation product after 72-96 hours.

3. Enzymolysis: adding 1kg of exogenous alkaline protease and 0.4 ton of water into 1 ton of fermented mixture, mixing uniformly, and performing enzymolysis at 50-60 deg.C for 8-12 hr.

4. Extracting polypeptide liquid: and (3) dehydrating the mixture after enzymolysis to obtain a polypeptide mixture with low water content and a polypeptide liquid.

5. Mixing the ingredients for the first time: adding 250-400kg astaxanthin into each ton of dehydrated polypeptide mixture, and mixing to obtain a first ingredient mixture.

6. Superfine grinding: micronizing the primary ingredient mixture.

7. And (3) secondary ingredient mixing: adding 20-60kg of phospholipid oil, 40-80kg of fish oil, 1-2kg of bile acid, 0.2-0.3kg of glutathione and 30-50kg of vitamin into each ton of the superfine pulverized primary ingredient mixture, and mixing to obtain a secondary ingredient mixture; the secondary ingredients are mixed and arranged after the superfine grinding, so that the unstable components are prevented from being damaged by the superfine grinding.

8. Puffing and granulating: and (4) putting the secondary ingredient mixture into a bulking machine for bulking and granulating to obtain bulked granules.

9. Drying and cooling: after the expanded granules are dried in a dryer, the moisture content is controlled to be less than 10%, and the drying temperature is controlled to be between 50 and 60 ℃, so that the damage to easily pyrolyzed components such as vitamins and the like is avoided; and then cooled by a cooler.

10. Vacuum spraying: active substances such as bacteria-enzyme composite preparation liquid and the like are uniformly sprayed on the surface of the expanded granules by adopting a vacuum spraying technology and permeate into the granules. The bacteria-enzyme composite preparation comprises a composite enzyme preparation, a fermentation culture medium and a composite bacteria preparation. Wherein the compound bacteria preparation is one or more of lactobacillus plantarum, enterococcus faecalis, saccharomyces cerevisiae, bacillus subtilis, bacillus licheniformis, bacillus megaterium, bacillus coagulans and clostridium butyricum; the fermentation medium comprises the following components in parts by weight: 10-20 parts of molasses, 3-5 parts of corn starch, 6-10 parts of glucose, 0.3-0.5 part of sodium chloride, 0.2-0.25 part of monopotassium phosphate, 0.2-0.25 part of ammonium dihydrogen phosphate, 0.15-0.2 part of magnesium sulfate, 0.004-0.005 part of ferrous sulfate, 0.5-1 part of tween 80 and 800-1000 parts of water; the complex enzyme preparation is one or more of phytase, neutral protease, amylase, pectinase, galactosidase, cellulase, glucanase, lipase, maltase and mannanase.

11. Constant-temperature anaerobic fermentation: under the sealing condition, the temperature is kept between 25 ℃ and 40 ℃, and the fermented soft pellet feed is obtained after 10 days to 15 days of fermentation. A large number of tests prove that in the anaerobic fermentation process, the mould (aerobic bacteria) is thoroughly killed, and the bacteria such as escherichia coli, salmonella and the like in the feed raw materials, which can survive under the anaerobic condition, are combined with bifidobacterium, lactobacillus siccatus, bacillus subtilis and bacillus licheniformis to act, so that oxygen is rapidly consumed, and an acidic environment is formed. Under anaerobic and acidic conditions, the growth and reproduction of harmful bacteria are inhibited until the harmful bacteria are killed, and further the pathogenic danger of pathogenic bacteria in the feed path is blocked. Therefore, antibiotics can be completely replaced, and antibiotic-free feeding is realized.

12. Screening, grading and packaging to form the finished feed.

Example 4

This example provides a process for preparing a biologically expanded compound feed containing polypeptides.

Screening and proportioning raw materials: crushing the fish and shrimp raw materials to 40 meshes, and filtering to obtain fish and shrimp pulp; and (3) taking 20 parts of fish and shrimp paste and 80 parts of peanut meal, and uniformly mixing and stirring to form a raw material mixture.

And (3) microbial fermentation: preparing a liquid strain, wherein: 108 CFU/g of bifidobacterium, 2 multiplied by 108 CFU/g of dry sour lactobacillus, 108 CFU/g of bacillus subtilis and 2 multiplied by 108 CFU/g of bacillus licheniformis; the raw material mixture and the liquid strain are mixed according to the proportion of 1:0.3 to form a microorganism-raw material mixture, fermenting at 36 ℃, and obtaining a fermentation product after 82 hours. Detecting indexes of the fermentation product: water 41.63%, small peptide 10.21%, pH 5.10, crude protein 26.26%.

Enzymolysis: 1kg of exogenous alkaline protease and 0.4 ton of water are added into every 1 ton of fermented mixture, and the mixture is uniformly mixed and subjected to enzymolysis for 12 hours at the temperature of 50 ℃.

Extracting polypeptide liquid: and (3) dehydrating the mixture after enzymolysis to obtain a polypeptide mixture with low water content and a polypeptide liquid.

Mixing the ingredients for the first time: adding 250kg astaxanthin per ton of polypeptide mixture, and mixing to obtain a first compounding mixture.

Superfine grinding: micronizing the primary ingredient mixture.

And (3) secondary ingredient mixing: adding 40kg of phospholipid oil, 60kg of fish oil, 1kg of bile acid, 0.2kg of glutathione and 40kg of vitamin into each ton of the superfine pulverized primary ingredient mixture, and mixing to obtain a secondary ingredient mixture.

Puffing and granulating: and (4) putting the secondary ingredient mixture into a bulking machine for bulking and granulating to obtain bulked granules.

Drying and cooling: the expanded granules are dried in a dryer, then the moisture content is controlled to be less than 10%, and then the expanded granules are cooled by a cooler.

Vacuum spraying: the bacteria-enzyme complex formulation liquid of example 3 was uniformly sprayed onto the surface of the expanded pellet and penetrated into the interior of the pellet using a vacuum spray technique.

Constant-temperature anaerobic fermentation: and (3) under a sealed condition, keeping the temperature at 30 ℃, and fermenting for 15 days to obtain the fermented soft pellet feed.

Screening, grading and packaging to form the finished feed.

Example 5

Screening and proportioning raw materials: crushing the fish and shrimp raw materials to 40 meshes, and filtering to obtain fish and shrimp pulp; taking 40 parts of fish and shrimp paste and 60 parts of peanut meal, and uniformly mixing and stirring to form a raw material mixture.

And (3) microbial fermentation: preparing a liquid strain, wherein: 108 CFU/g of bifidobacterium, 2 multiplied by 108 CFU/g of dry sour lactobacillus, 108 CFU/g of bacillus subtilis and 2 multiplied by 108 CFU/g of bacillus licheniformis; the raw material mixture and the liquid strain are mixed according to the proportion of 1:0.3 to form a microorganism-raw material mixture, fermenting at 39 ℃ for 96 hours to obtain a fermented product. Detecting indexes of the fermentation product: water 41.78%, small peptide 10.42%, pH 5.03, crude protein 26.07%.

Enzymolysis: 1kg of exogenous alkaline protease and 0.4 ton of water are added into every 1 ton of fermented mixture, and the mixture is uniformly mixed and subjected to enzymolysis for 8 hours at the temperature of 60 ℃.

Mixing the ingredients for the first time: adding 400kg astaxanthin per ton polypeptide mixture, and mixing to obtain a first compounding mixture.

Superfine grinding: micronizing the primary ingredient mixture.

And (3) secondary ingredient mixing: 60kg of phospholipid oil, 80kg of fish oil, 2kg of bile acid, 0.3kg of glutathione and 50kg of vitamin are added into each ton of the superfine pulverized primary ingredient mixture and mixed to obtain a secondary ingredient mixture.

Constant-temperature anaerobic fermentation: and (3) under a sealed condition, keeping the temperature at 25 ℃, and fermenting for 13 days to obtain the fermented soft pellet feed.

Screening, grading and packaging to form the finished feed.

Example 6

Screening and proportioning raw materials: crushing the fish and shrimp raw materials to 40 meshes, and filtering to obtain fish and shrimp pulp; and (3) taking 30 parts of fish and shrimp paste and 70 parts of peanut meal, and uniformly mixing and stirring to form a raw material mixture.

And (3) microbial fermentation: preparing a liquid strain, wherein: 108 CFU/g of bifidobacterium, 2 multiplied by 108 CFU/g of dry sour lactobacillus, 108 CFU/g of bacillus subtilis and 2 multiplied by 108 CFU/g of bacillus licheniformis; the raw material mixture and the liquid strain are mixed according to the proportion of 1:0.3 to form a microorganism-raw material mixture, fermenting at 34 deg.C for 84 hr to obtain a fermented product. Detecting indexes of the fermentation product: water 41.72%, small peptide 10.36%, pH 5.03, crude protein 26.13%.

Enzymolysis: 1kg of exogenous alkaline protease and 0.4 ton of water are added into every 1 ton of fermented mixture, and the mixture is uniformly mixed and subjected to enzymolysis for 10 hours at the temperature of 55 ℃.

Extracting polypeptide liquid: and dehydrating the mixture after enzymolysis to obtain a polypeptide mixture with low water content and a polypeptide liquid.

Mixing the ingredients for the first time: adding 350kg astaxanthin per ton polypeptide mixture, and mixing to obtain a first compounding mixture.

Superfine grinding: micronizing the primary ingredient mixture.

And (3) secondary ingredient mixing: adding phospholipid oil 20kg, fish oil 40kg, bile acid 2kg, glutathione 0.3kg and vitamins 40kg into each ton of the superfine pulverized primary ingredient mixture, and mixing to obtain a secondary ingredient mixture.

Constant-temperature anaerobic fermentation: and (3) under a sealed condition, keeping the temperature at 40 ℃, and fermenting for 10 days to obtain the fermented soft pellet feed.

Screening, grading and packaging to form the finished feed.

Example 7

And (3) microbial fermentation: preparing a liquid strain, wherein: 108 CFU/g of bifidobacterium, 2 multiplied by 108 CFU/g of dry sour lactobacillus, 108 CFU/g of bacillus subtilis and 2 multiplied by 108 CFU/g of bacillus licheniformis; the raw material mixture and the liquid strain are mixed according to the proportion of 1:0.3 to form a microorganism-raw material mixture, fermenting at 37 ℃ for 72 hours to obtain a fermented product. Detecting indexes of the fermentation product: 41.75% of water, 10.44% of small peptide, 5.04% of pH and 26.02% of crude protein.

Enzymolysis: 1kg of exogenous alkaline protease and 0.4 ton of water are added into every 1 ton of fermented mixture, and the mixture is uniformly mixed and subjected to enzymolysis for 9 hours at the temperature of 55 ℃.

Superfine grinding: micronizing the primary ingredient mixture.

And (3) secondary ingredient mixing: adding 40kg of phospholipid oil, 60kg of fish oil, 2kg of bile acid, 0.3kg of glutathione and 40kg of vitamin into each ton of the superfine pulverized primary ingredient mixture, and mixing to obtain a secondary ingredient mixture.

Constant-temperature anaerobic fermentation: and (3) under a sealed condition, keeping the temperature at 35 ℃, and fermenting for 13 days to obtain the fermented soft pellet feed.

Screening, grading and packaging to form the finished feed.

Effect investigation:

investigation is one:

in order to research the effect of the biological fermentation puffed feed instead of industrial aquaculture feed for litopenaeus vannamei, the research carries out a growth contrast test of the biological fermentation puffed feed and common commercial feed. Three experimental groups A, B, C are set in an experiment of a certain industrial prawn culture base, wherein A is low-protein commercial feed, B is biological fermentation puffed feed and C is high-protein commercial feed. The 9 culture ponds are randomly divided into three groups, each group is repeated by 3, and 8000-10000 healthy litopenaeus vannamei shrimps are placed in each culture pond. During the test period, the shrimp were fed 4% of the shrimp weight daily, four times daily, at 06:00, 10:00, 16:00, 20:00 and recording water quality parameters such as feeding amount, water temperature, pH, salinity, dissolved oxygen and the like. And a bait platform is arranged in the pond, and after the bait is thrown for 1 hour, the ingestion condition of the prawns is observed. And randomly fishing 20 litopenaeus vannamei tails from each test pool of the industrial prawn culture base at intervals of 30d, measuring the body length and the body weight of the litopenaeus vannamei tails, and calculating the average value. And (4) in the shrimp outlet season after the culture is finished, counting the total output of each pond of the industrial prawn culture base.

The test result shows that the group B adopting the biological fermentation expanded feed has no significant difference with the group C with high protein in terms of the tail weight, the weight gain rate, the specific growth rate, the body composition, the TOR gene expression level and the like of the litopenaeus vannamei, the group B is obviously superior to the group A with low protein in all growth indexes of the litopenaeus vannamei, and all indexes of the group C are between the group A and the group B. The biological fermentation expanded feed group shows a growth effect similar to that of high-protein feed and is obviously superior to low-protein feed. Therefore, the biological fermentation expanded feed can replace the total part of high protein feed for the culture of litopenaeus vannamei to feed. After technicians in the culture field react and insist on feeding the biologically fermented puffed feed for 2-3 weeks, the prawns have the advantages of vigorous appetite, obviously increased feed intake, obviously accelerated growth speed, obviously enhanced vitality, transparent shrimp bodies, solid meat and clear liver and pancreas edges. Practice proves that the biological fermentation puffed feed is fed in the whole process and is matched with a water quality regulation and control technology advocated by a prawn ecological breeding mode, the prawn body is obviously robust, and the immunity, stress resistance and stress resistance of the prawn are obviously improved.

And B, investigation II:

in order to research the effect of the biological fermentation expanded feed for replacing the feed for industrially culturing the penaeus vannamei boone, a culture comparison test is carried out in a certain penaeus vannamei boone demonstration base. Through two-year production comparison, the average weight of the prawns in a test group (the biological fermentation expanded feed of the invention) is 17.1g, and the average body length is 11.8cm, and the average weight of a control group (a certain high-protein commercial feed) is 15.2g, and the average body length is 11.1cm; the yield per unit area of a test group is 5.69kg per square meter, the yield per unit area of a comparison group is 5.12kg per square meter, and the yield of the test group is increased by 11.13 percent compared with that of the comparison group.

It is to be understood that the invention is not limited to the specific embodiments described above, but is intended to cover various insubstantial modifications of the inventive process concepts and solutions, or its application to other applications without modification.

Claims

1. The utility model provides a biological popped formula feed's of polypeptide preparation facilities, includes jar body (1) and popped conveyer (11), its characterized in that, jar body (1) includes:

the first feeding port (2) is arranged at the upper end of the tank body (1); the first charging opening (2) is provided with

The charging door (3) can control the opening and closing of the first charging opening (2);

the middle partition plate (4) is fixed in the middle of the tank body (1);

the stirring bin (5) is arranged above the middle partition plate (4); the lower end of the stirring bin (5) is provided with

The first discharge hole (6) is used for communicating the stirring bin (5) with the lower part of the middle partition plate (4);

the discharge valve (7) is arranged at the first discharge hole (6);

the grinding bin (8) is arranged below the first discharge hole (6) and is rotatably connected with the side wall of the tank body (1);

the output end of the second motor (9) is fixedly connected with the grinding bin (8);

the second discharge hole (10) is formed in the bottom of the tank body (1);

the lower part of the second discharge hole (10) is communicated with the inlet of the bulking conveyor (11);

the side wall of the stirring bin (5) is made of a water-permeable material, a gap is reserved between the side wall of the stirring bin (5) and the side wall of the tank body (1), a liquid outlet (14) is formed in the side wall of the tank body (1) and is tightly attached to the upper portion of the middle partition plate (4), and a liquid outlet valve (15) is arranged on the liquid outlet (14);

a first motor (16) is arranged in the middle of the upper end of the tank body (1), the output end of the first motor (16) is connected with a stirring rod (17) and a plurality of telescopic rods (18), and a pressing plate (19) is fixed at the lower end of each telescopic rod (18);

a second charging hole (21) is formed between the second discharging hole (10) and the bulking conveyor (11);

the use method of the preparation device comprises the following steps:

opening a feed door (3), adding quantitative fermentation raw materials and liquid strains into the stirring bin (5) from the first feed port (2), and closing the feed door (3);

starting a first motor (16) to drive a stirring rod (17) to stir, mix and ferment the materials in the stirring bin (5); after fermentation is completed, opening the feed door (3), adding enzyme and water, then closing the feed door (3), starting the first motor (16) to stir again for enzymolysis; after enzymolysis is finished, the liquid outlet valve (15) is opened, each telescopic rod (18) is started, each pressing plate (19) is pushed to extrude the materials in the stirring bin (5) from top to bottom, and partial liquid penetrates through the side wall of the stirring bin (5) and then flows out from the liquid outlet (14);

opening the feed door (3), and adding the ingredients mixed by the primary ingredients; closing the charging door (3), and starting the first motor (16) to stir and mix; opening a discharge valve (7), and allowing part of the polypeptide mixture to fall into a grinding bin (8) from the stirring bin (5); closing the discharge valve (7), and carrying out ultramicro crushing on the materials in the grinding bin (8); after the grinding is finished, a second motor (9) is started to drive the grinding bin (8) to rotate for 180 degrees, and the ground material falls into the bottom of the tank body (1); the puffing conveyor (11) is started, materials are continuously taken away from the bottom of the tank body (1), meanwhile, liquid ingredients mixed by the secondary ingredients are continuously added from the second feeding port (21), and the puffing granular materials are output by the puffing conveyor (11).

2. The device for preparing the polypeptide biological puffed compound feed according to claim 1, characterized by further comprising an air blower (12), wherein the inlet of the air blower (12) is communicated with the upper part of the tail end of the puffing conveyor (11), and the outlet is respectively communicated with the stirring bin (5) and the side wall of the tank body (1) between the grinding bin (8) and the second discharge hole (10).

3. The device for preparing the polypeptide biological puffed compound feed as claimed in claim 2, wherein the output end of the blower (12) is provided with an electric heater (13).

4. The device for preparing the polypeptide biological puffed compound feed according to claim 1, wherein the number of the first feeding ports (2) is two, and the two feeding ports are respectively positioned at two ends of the top of the tank body (1); the feeding door (3) comprises a circular sliding ring (301), a groove matched with the sliding ring (301) is formed in the top of the tank body (1), and the sliding ring (301) is in sliding contact with the groove; two ends of the sliding ring (301) are fixedly connected with two shielding plates (302) respectively.

5. The device for preparing the polypeptide biological puffed compound feed according to claim 1, wherein a grinding disc (20) is arranged in the grinding bin (8), a gravity sensor is arranged below the grinding disc (20), and when the weight detected by the gravity sensor reaches a set value, the first discharge hole (6) is closed.

6. The apparatus for preparing a biological puffed compound polypeptide feed as claimed in claim 1, wherein the liquid bacterial strain comprises: bifidobacterium 108 CFU/g, lactobacillus acidovorus 2X 108 CFU/g, bacillus subtilis 108 CFU/g, bacillus licheniformis 2X 108 CFU/g.

7. The apparatus for preparing a biologically expanded compound feed containing polypeptide according to claim 1, wherein the secondary ingredient mixing comprises: adding phospholipid oil 20-60kg, fish oil 40-80kg, bile acid 1-2kg, glutathione 0.2-0.3kg, and vitamins 30-50kg into each ton of the superfine pulverized mixture.