CN114940631B

CN114940631B - Compound microbial fertilizer and LTC nutrition chelating production process thereof

Info

Publication number: CN114940631B
Application number: CN202210405138.4A
Authority: CN
Inventors: 马海燕; 屈庆涛; 屈凡河; 赵越
Original assignee: Beijing Zhongnongfuyuan Group Co ltd
Current assignee: Beijing Zhongnongfuyuan Group Co ltd
Priority date: 2021-04-19
Filing date: 2022-04-18
Publication date: 2023-04-25
Anticipated expiration: 2042-04-18
Also published as: CN114940631A; CN113045358A

Abstract

The application relates to the field of fertilizer processing, and in particular discloses a compound microbial fertilizer and an LTC nutrition chelating production process thereof, wherein the compound microbial fertilizer is prepared from the following raw materials in parts by weight: composite bacterial, medium trace element powder, amino acid liquid, fulvic acid, humic acid, macroelement powder, plant extract, seaweed chitin, chitosan oligosaccharide, vitamin and sterile water; the preparation method comprises the following steps: weighing medium trace elements, amino acid liquid, fulvic acid, humic acid, macroelement powder, plant extract, seaweed chitin, chitosan oligosaccharide, vitamins and sterile water, mixing uniformly, sterilizing at 115-125deg.C for 25-35min, cooling to 32-38deg.C, and making into mixed solution; weighing composite strains, mixing with the mixed solution, chelating for 100-150min at constant temperature, sampling, detecting, and filling after the composite strains are qualified to obtain a finished fertilizer; has the advantages of high yield and high quality of crops.

Description

Compound microbial fertilizer and LTC nutrition chelating production process thereof

Technical Field

The application relates to the field of fertilizer processing, in particular to a compound microbial fertilizer and an LTC nutrition chelating production process thereof.

Background

The microbial fertilizer industry has no better nutrition chelation technology so far, generally uses single strain and organic matters as main nutrients, namely a production mode of strain water mixing, or adds some liquid humic acid so as to increase the organic matter content, and uniformly stirring the elements in a manual or mechanical mode to form a liquid microbial fertilizer product.

Therefore, the general liquid microbial fertilizer has a very simple formula, single nutrient components and microorganisms distributed in a dispersed form are difficult to survive in nitrogen, phosphorus and potassium and other nutrients, so that the effective viable bacteria content is difficult to ensure, and the process is simple and easy to infect mixed bacteria, so that the fertilizer effect is influenced.

Along with the current planting difficulty in China agriculture and the advocacy of a green ecological agriculture planting mode, the traditional microbial fertilizer product can not meet the demands of China agriculture, so that the biological fertilizer product is needed to be provided, and the planted crops are high in yield and quality.

Disclosure of Invention

In order to ensure that the planted crops have high yield and high quality, the application provides a compound microbial fertilizer and an LTC nutrition chelating production process thereof.

In a first aspect, the present application provides a compound microbial fertilizer, which adopts the following technical scheme:

A compound microbial fertilizer is prepared from the following raw materials in parts by weight: 0.35-1.3 parts of composite strain, 1-5 parts of medium trace element powder, 3-8 parts of amino acid liquid, 1-5 parts of fulvic acid, 2-7 parts of humic acid, 15-25 parts of macroelement powder, 10-20 parts of plant extract, 2-6 parts of seaweed chitin, 3-7 parts of chitosan oligosaccharide, 0.5-1 part of vitamin and 100-200 parts of sterile water.

By adopting the technical scheme, the compound bacteria are chelated by utilizing amino acid liquid, humic acid, fulvic acid, chitosan oligosaccharide and seaweed chitin, and the chelating bacteria still maintain higher activity and strong vitality in the finished fertilizer by matching with medium trace element powder, macroelement powder, plant extract and vitamins, so that the multi-effect integrated and comprehensive-nutrition fertilizer is formed, thereby enabling crops to have high yield and high quality.

The medium trace element powder, the macroelement powder and the vitamins are matched to provide rich nutrients for the soil, so that the absorption of the nutrients by crops is improved, and the crops are high in yield and quality from the perspective of improving the nutrients; the composite strain and the seaweed chitin are matched, so that the effects of nourishing, protecting, increasing, strengthening, preventing and controlling red roots, black roots, rotten roots, dying plants and the like can be achieved, the root systems of crops are well protected, and nutrient substances are better absorbed, so that the quality of the crops is improved, and the yield of the crops is increased; the amino acid liquid, the humic acid and the fulvic acid are matched to play a role in resisting salt and alkali, so that the pH value of soil can be regulated, and the growth of root systems is promoted by building a good soil environment, thereby ensuring good growth of crops, improving the quality of the crops and promoting the yield increase of the crops; the plant extract, the composite strain and the chitosan oligosaccharide are matched, so that continuous cropping, nematode inhibition, drought resistance, cold resistance and dry heat resistance can be realized, the crop stress resistance is improved, the crop growth is prevented from being influenced by the external environment, the crop quality is improved, and the crop yield is increased.

Preferably, the composite strain consists of trichoderma viride, bacillus subtilis, bacillus licheniformis and 5406 actinomycetes in a weight ratio of 1:1-3:1-1.5:0.5-1.

By adopting the technical scheme, the trichoderma viride, the bacillus subtilis, the bacillus licheniformis and the 5406 actinomycetes are matched, and the fertilizer has the advantages of preventing and controlling various soil-borne diseases, killing pests, preventing and controlling the pests, promoting the growth of crops, improving the stress resistance of the crops, improving the soil structure, degrading harmful substances in the soil, decomposing nitrogen, phosphorus and potassium, enhancing the fertilizer efficiency and the like, so that the growth of the crops is promoted, and the crops are high in yield and quality.

The trichoderma viride can directly kill root knot nematodes and underground pests in the roots and soil of crops, can kill plough layer germs and pests, and can inhibit various plant mycoses, such as soil-borne diseases of root rot, damping-off, blight and the like; the bacillus subtilis is metabolized to produce active substances such as subtilisin, polymyxin, gramicidin and the like, which have an inhibitory effect on pathogenic bacteria, can increase soil nutrients, improve soil structure and promote organic matters in soil to decompose into humus, thereby improving the yield and quality of crops; the bacillus licheniformis can inhibit the reproduction of pathogenic bacteria in soil and reduce plant soil-borne diseases; the germination rate and the seedling protection rate of the seeds are improved, the genetic diseases of the seeds are prevented, the survival rate of crops is improved, and the root growth is promoted; improving soil, improving soil aggregate structure and improving soil water storage capacity; 5406 actinomycetes have nitrogen fixation, phosphorus dissolution, potassium release, disease resistance and other functions, and can degrade substances which are difficult to decompose, such as lignin, cellulose, chitin and the like, and actinomycetes can promote the proliferation of azotobacter; the fertilizer is absorbed by the root system through protecting the root system of the crops and promoting the root system, so that the crops have the advantages of high yield and high quality.

Preferably, the medium trace element powder consists of calcium sulfate, magnesium sulfate and silicon dioxide in a weight ratio of 1:0.6-1.2:0.4-1.

By adopting the technical scheme, the calcium sulfate, the magnesium sulfate and the silicon dioxide are matched to provide the calcium element, the magnesium element, the sulfur element and the silicon element for the fertilizer, and the compound strain is convenient to chelate with the medium trace element powder, so that the crops are high in yield and high in quality, and the medium trace element powder can provide a large amount of nutrient substances for the crops, thereby promoting the growth of the crops and enabling the crops to be high in yield and high in quality.

Preferably, the macroelement powder consists of potassium phosphate, monopotassium phosphate, ammonium nitrate and ammonium sulfate in a weight ratio of 1:1-2:0.5-1.5:1-2.

By adopting the technical scheme, the potassium phosphate, the monopotassium phosphate, the ammonium nitrate and the ammonium sulfate are matched to provide potassium element, nitrogen element and phosphorus element for the fertilizer, so that the compound strain is convenient to chelate with the macroelement powder, thereby ensuring that the crops are high in yield and high in quality, and the macroelement powder can provide a large amount of nutrient substances for the crops, thereby promoting the growth of the crops and ensuring that the crops are high in yield and high in quality.

Preferably, the plant extract consists of oleander extract, malachite extract and sesbania gum in the weight ratio of 1:1-2:0.1-0.5.

By adopting the technical scheme, the oleander extract, the malachite extract and the sesbania gum are matched, so that the insecticidal composition has a good effect of inhibiting and killing insects.

Preferably, the fertilizer further comprises the following raw materials in parts by weight: 3-7 parts of modified bamboo fiber.

By adopting the technical scheme, the modified bamboo fiber, the composite strain, the amino acid liquid, the seaweed chitin and the chitosan oligosaccharide are matched, the amino acid liquid, the seaweed chitin and the chitosan can chelate the composite strain, the better water absorption effect of the bamboo fiber is utilized, so that the chelate strain in the finished fertilizer is attached to the surface of the bamboo fiber, when the fertilizer is added into soil, the bamboo fiber can not only provide moisture and oxygen for the growth and propagation of the composite strain, but also provide nutrient substances, promote the growth and propagation of the composite strain, and improve the quantity of the composite strain in the soil, thereby promoting the high quality and high yield of crops.

The modified bamboo fiber, the bacillus licheniformis and the chitosan oligosaccharide are matched, a large amount of preliminary water storage can be realized by utilizing the strong water absorption effect of the bamboo fiber, the soil is greatly water-retaining by matching with the improvement effect of the bacillus licheniformis, and crops are promoted to have high water absorption and water retention capacity by matching with the chitosan oligosaccharide, so that the crops have good drought resistance effect, and the yield of the crops can be improved and the crops are high in quality under drought conditions.

Preferably, the modified bamboo fiber is prepared by the following method:

weighing 5-15 parts of bamboo fibers, adding 35-60 parts of sodium hydroxide solution, reacting for 1-5min, and then adding 35-60 parts of dilute sulfuric acid, and continuously reacting for 1-5min to obtain pretreated bamboo fibers;

II, weighing 0.3-0.8 part of ceric ammonium nitrate, 20-50 parts of acrylamide and 3-9 parts of modified silicon dioxide, adding the ceric ammonium nitrate, the acrylamide and the modified silicon dioxide into the pretreated bamboo fiber prepared by the I, and stirring for 2-4.5 hours at 55-80 ℃ to prepare a semi-finished product;

III, washing the semi-finished product prepared in the step II to be neutral, dehydrating by absolute ethyl alcohol, drying, crushing and vacuum drying to prepare the modified bamboo fiber.

By adopting the technical scheme, the bamboo fiber, the acrylamide and the modified silicon dioxide are matched, and the amino group of the acrylamide is matched with the bamboo fiber to have larger specific surface area, so that the amino group and the hydroxyl group on the surface of the chelate are conveniently attracted and connected, the chelate is conveniently attached to the surface of the modified bamboo fiber, and the self weight of the modified bamboo fiber can be increased; meanwhile, the weight of the bamboo fiber is further increased by matching with modified silicon dioxide attached to the surface of the bamboo fiber; during rainfall, the chelating bacteria and other components in the fertilizer are prevented from being washed away by the rainwater as much as possible, so that the growth of crops is ensured.

Bamboo fiber, acrylamide and field green gum are matched; the surface area of the grafted bamboo fiber is increased, a plurality of grafting sites of the bamboo fiber are convenient to contact with the chelate, and the chelate contacted with the bamboo fiber can be firmly adhered to the surface of the modified bamboo fiber by matching with the good water-soluble cohesiveness of acrylamide and sesbania gum, so that the modified bamboo fiber can better wrap and fix the chelate, and the fertilizer is prevented from being washed away by rainwater due to rainfall after fertilization, thereby ensuring the growth of crops.

Preferably, the modified silicon dioxide is prepared by the following method: weighing 20-35 parts of silicon dioxide, placing the silicon dioxide in 55-75 parts of fluorosilane solution, performing ultrasonic dispersion for 5-10min, taking out the silicon dioxide, and drying at room temperature to obtain the modified silicon dioxide.

Through adopting above-mentioned technical scheme, the hydrophobic modification effect of silica through fluorosilane solution for the silica has hydrophobic effect, and when the rainfall, the hydrophobic effect that utilizes the silica reduces the contact of rainwater and modified bamboo fibre, thereby most rainwater directly flows away with the help of the hydrophobic effect of silica, and is not contacted with modified bamboo fibre, thereby reduces the contact of rainwater and chelate, and when the rainfall after the fertilization, the fertilizer in the soil is taken away to the as far as possible to the rainwater is avoided, thereby guarantees the growth of crop.

Preferably, the fertilizer also comprises 2-6 parts of coated perlite, and the coated perlite is prepared by the following method: weighing carboxymethyl cellulose, dispersing the carboxymethyl cellulose into the open-cell expanded perlite, wherein the weight ratio of the carboxymethyl cellulose to the open-cell expanded perlite is 0.5-1:1, and preparing the loaded perlite; and uniformly spraying an ethyl cellulose solution on the surface of the loaded perlite, wherein the mass ratio of the ethyl cellulose solution to the loaded perlite is 0.1-0.35:1, and drying to obtain the coated perlite.

By adopting the technical scheme, the carboxymethyl cellulose, the open-pore expanded perlite and the ethylcellulose solution are matched, the carboxymethyl cellulose is filled into the pores of the open-pore expanded perlite, then the ethylcellulose solution is used for coating, and an ethylcellulose film is formed on the surface of the open-pore expanded perlite, so that the coated perlite is prepared.

In the fertilizer preparation process, the ethyl cellulose membrane separates the hole expanded perlite to absorb water in the liquid fertilizer, after the coated perlite is added into the soil, the bacteria in the soil are matched with the compound bacteria in the fertilizer to decompose the ethyl cellulose membrane, and the degraded ethyl cellulose can provide energy substances for the bacteria to promote the growth and propagation of the bacteria, so that more bacteria are attached to the surface of the coated perlite; with the decomposition of the ethylcellulose film on the surface of the coated perlite and the incomplete coating of the pores, the open-pore expanded perlite gradually absorbs water slowly, and the carboxymethyl cellulose in the open-pore expanded perlite is slowly dissolved in water, so that when rainfall occurs, the carboxymethyl cellulose is dissolved to generate viscosity, the coated perlite is promoted to be adhered to a chelate close to the coated perlite, and the composite strain in the soil and other raw materials in the fertilizer are prevented from being washed away by rainfall as much as possible, thereby ensuring the growth of crops.

In a second aspect, the application provides a LTC nutrition chelating production process of a compound microbial fertilizer, which adopts the following technical scheme:

an LTC nutrition chelation production process of a compound microbial fertilizer comprises the following steps:

s1, weighing medium trace elements, amino acid liquid, fulvic acid, humic acid, macroelement powder, plant extract, seaweed chitin, chitosan oligosaccharide, vitamins and sterile water, uniformly mixing, sterilizing at 115-125 ℃ for 25-35min, and cooling to 32-38 ℃ to obtain a mixed solution;

s2, weighing the composite strain, mixing with the mixed solution, chelating for 100-150min at constant temperature, sampling, detecting, and filling after the composite strain is qualified to obtain the finished fertilizer.

By adopting the technical scheme, the nutrients are sterilized at high temperature and then inoculated with composite strains, so that the purity of beneficial strains is ensured; the microorganisms are chelated in the nutrient raw materials through constant-temperature chelation, so that good activity and strong vitality are maintained, and the multi-effect integrated and comprehensive-nutrition fertilizer is formed, thereby ensuring high yield and high quality of crops.

In summary, the present application has the following beneficial effects:

1. the compound bacteria are chelated by utilizing the chelating effect of amino acid liquid, humic acid, fulvic acid, seaweed chitin and chitosan oligosaccharide, and the better connecting effect of plant extract is matched, so that the chelated bacteria are contacted with nutrient elements and maintain the activity of the chelated bacteria, and the chelated bacteria still maintain good activity and strong vitality in the finished fertilizer, thus forming the multi-effect integrated and comprehensive-nutrition fertilizer, and promoting the high yield and high quality of crops.

2. The composite strain, the medium trace element powder, the amino acid liquid, the fulvic acid, the humic acid, the macroelement powder, the plant extract, the seaweed chitin, the chitosan oligosaccharide and the vitamin are utilized to be matched, rich nutrient substances are provided for crops to be absorbed firstly, then the crop root system is protected, the crop root system can absorb the nutrient substances better, a good soil environment is built again, the crop growth is promoted, finally the stress resistance of the crop is improved, the influence of the external environment in the crop growth process is avoided, and therefore the effects of improving the crop quality and promoting the crop yield are achieved.

3. The compound bacteria, the medium trace element powder, the amino acid liquid, the fulvic acid, the humic acid, the macroelement powder, the plant extract, the seaweed chitin, the chitosan oligosaccharide and the vitamin are matched, so that the fertilizer has the function of degrading pesticide residues, and the prepared fertilizer has wide applicability and is suitable for various soils and various crops.

4. The prepared fertilizer is convenient to apply, good in effect and durable in fertilizer efficiency, and can effectively reduce the input cost of a grower.

5. The modified bamboo fiber, the coated perlite, the amino acid liquid, the seaweed chitin, the chitosan oligosaccharide and the composite strain are matched, the amino acid liquid, the seaweed chitin and the chitosan oligosaccharide have chelation effect on the composite strain, amino groups and hydroxyl groups on the surface of the chelate are matched with amino groups and hydroxyl groups on the surface of the modified bamboo fiber, the coated perlite is promoted to contact and bond the chelate, and when rainfall occurs, the chelate in the fertilizer is further bound, so that the fertilizer is prevented from being carried away with water, and the growth of crops is ensured.

Detailed Description

The present application is described in further detail below with reference to examples.

Preparation example of modified silica

Silica in the following raw materials was purchased from southwest english-rayleigh biotechnology limited; fluorosilane is purchased from Zhengzhou macro chemical industry Co., ltd.) and has a content of 97%; other raw materials and equipment are all commonly and commercially available.

Preparation example 1: the modified silicon dioxide is prepared by the following method:

28kg of silicon dioxide is weighed and placed in 65kg of fluorosilane solution with the mass fraction of 2%, ultrasonic dispersion is carried out for 8min, the silicon dioxide is taken out, and the modified silicon dioxide is prepared after the silicon dioxide is dried at room temperature, wherein the particle size of the silicon dioxide is 20 meshes.

Preparation example 2: the modified silicon dioxide is prepared by the following method:

20kg of silicon dioxide is weighed and placed in 55kg of fluorosilane solution with the mass fraction of 2%, ultrasonic dispersion is carried out for 5min, the silicon dioxide is taken out, and the modified silicon dioxide is prepared after the silicon dioxide is dried at room temperature, wherein the particle size of the silicon dioxide is 20 meshes.

Preparation example 3: the modified silicon dioxide is prepared by the following method:

35kg of silicon dioxide is weighed and placed in 75kg of fluorosilane solution with the mass fraction of 2%, ultrasonic dispersion is carried out for 10min, the silicon dioxide is taken out, and the modified silicon dioxide is prepared after the silicon dioxide is dried at room temperature, wherein the particle size of the silicon dioxide is 20 meshes.

Preparation example of modified bamboo fiber bamboo scraps of raw materials of which bamboo scraps are purchased from moso bamboo scraps processed by bamboos in Anhui Huning mountain areas; acrylamide is purchased from Shanghai national pharmaceutical group chemical reagent Co., ltd., CP grade; ceric ammonium nitrate is purchased from Shanghai national pharmaceutical group chemical reagent Co., ltd, grade AP; other raw materials and equipment are all commonly and commercially available.

Preparation example 4: the modified bamboo fiber is prepared by the following method:

placing bamboo scraps in a sodium hydroxide solution with the mass fraction of 40%, treating the bamboo scraps in an oil bath at the temperature of 110 ℃ for 2.2 hours, rinsing the bamboo scraps to be neutral with clear water, and drying the bamboo scraps to be constant weight to obtain bamboo fibers;

II, weighing 10kg of the bamboo fibers prepared by the method I, adding the bamboo fibers into 45kg of 1mol/L sodium hydroxide solution for reaction for 3min, and then adding 45kg of 10% dilute sulfuric acid with mass fraction for continuous reaction for 3min to prepare pretreated bamboo fibers;

III, weighing 0.5kg of ammonium cerium nitrate, 35kg of acrylamide and 6kg of modified silicon dioxide prepared in preparation example 1, adding the ammonium cerium nitrate, 35kg of acrylamide and 6kg of modified silicon dioxide into the pretreated bamboo fibers prepared in preparation example 1, and stirring at a water bath condition of 65 ℃ at a rotating speed of 500r/min for 3 hours to prepare a semi-finished product;

IV, washing the semi-finished product prepared by III to be neutral, dehydrating by absolute ethyl alcohol, drying, crushing and vacuum drying to prepare the modified bamboo fiber.

Preparation example 5: the modified bamboo fiber is prepared by the following method:

placing bamboo scraps in 40% sodium hydroxide solution, treating the bamboo scraps in an oil bath at 100 ℃ for 2.5 hours, rinsing the bamboo scraps with clear water to be neutral, and drying the bamboo scraps to constant weight to obtain bamboo fibers;

II, weighing 5kg of the bamboo fibers prepared by the method I, adding the bamboo fibers into 35kg of 1mol/L sodium hydroxide solution, reacting for 1min, and then adding 35kg of 10% dilute sulfuric acid by mass fraction, and continuously reacting for 1min to prepare pretreated bamboo fibers;

III, weighing 0.3kg of ammonium cerium nitrate, 20kg of acrylamide and 3kg of modified silicon dioxide prepared in preparation example 2, adding the ammonium cerium nitrate, the acrylamide and the modified silicon dioxide into the pretreated bamboo fibers prepared in II, and stirring at a water bath condition of 55 ℃ at a rotating speed of 500r/min for 4.5 hours to prepare a semi-finished product;

Preparation example 6: the modified bamboo fiber is prepared by the following method:

placing bamboo scraps in 40% sodium hydroxide solution, treating for 2 hours in an oil bath at 120 ℃, rinsing with clear water to be neutral, and drying to constant weight to obtain bamboo fibers;

II, weighing 15kg of the bamboo fibers prepared by the method I, adding the bamboo fibers into 60kg of 1mol/L sodium hydroxide solution for reaction for 5min, and then adding 60kg of 10% dilute sulfuric acid with mass fraction for continuous reaction for 5min to prepare pretreated bamboo fibers;

III, weighing 0.8kg of ammonium cerium nitrate, 50kg of acrylamide and 9kg of modified silicon dioxide prepared in preparation example 3, adding the ammonium cerium nitrate, 50kg of acrylamide and 9kg of modified silicon dioxide into the pretreated bamboo fiber prepared in preparation example 3, and stirring at a water bath condition of 80 ℃ at a rotating speed of 500r/min for 2 hours to prepare a semi-finished product;

Preparation example 7: the modified bamboo fiber is prepared by the following method:

and I, placing bamboo scraps in a 40% sodium hydroxide solution, treating the bamboo scraps in an oil bath at 120 ℃ for 2 hours, rinsing the bamboo scraps with clear water to be neutral, and drying the bamboo scraps to constant weight to obtain the modified bamboo fibers.

Preparation example of oleander extract

Oleander extract of the following raw materials was purchased from western security australite biotechnology limited.

Preparation example 8: weighing 10kg of oleander extract, placing in 100kg of 10% ethanol water solution, and stirring for 5min at 800r/min to obtain oleander extract.

Preparation example of Peacock grass extract

The malachite extracts of the following materials were purchased from the company of the biological engineering, siam Rayleigh.

Preparation example 9: 10kg of the malachite extract is weighed and placed in 100kg of an ethanol water solution with the mass fraction of 15 percent, and the malachite extract is stirred for 5min under the condition of 800r/min to prepare the malachite extract.

Preparation example of coated perlite

The following raw materials are all commercially available.

Preparation example 10: the coated perlite is prepared by the following steps:

weighing 0.75kg of carboxymethyl cellulose, dispersing into 99% absolute ethyl alcohol by mass fraction, adding into 1kg of open-cell expanded perlite, wherein the particle size of the carboxymethyl cellulose is 80nm, the particle size of the open-cell expanded perlite is 20 meshes, performing ultrasonic dispersion for 10min under the condition of 20kHz, and drying to obtain loaded perlite; and (3) weighing 0.2kg of ethyl cellulose solution, uniformly spraying the ethyl cellulose solution on the surface of 1kg of perlite, wherein the ethyl cellulose solution is ethyl cellulose solution with the mass fraction of 5%, the solvent of the ethyl cellulose solution is a mixed solvent of toluene/ethanol of 1/4, and drying to obtain the coated perlite.

Preparation example 11: the coated perlite is prepared by the following steps:

weighing 0.5kg of carboxymethyl cellulose, dispersing into 99% absolute ethyl alcohol by mass fraction, adding into 1kg of open-cell expanded perlite, wherein the particle size of the carboxymethyl cellulose is 80nm, the particle size of the open-cell expanded perlite is 20 meshes, performing ultrasonic dispersion for 10min under the condition of 20kHz, and drying to obtain loaded perlite; and (3) weighing 0.1kg of ethyl cellulose solution, uniformly spraying the ethyl cellulose solution on the surface of 1kg of perlite, wherein the ethyl cellulose solution is ethyl cellulose solution with the mass fraction of 5%, the solvent of the ethyl cellulose solution is a mixed solvent of toluene/ethanol of 1/4, and drying to obtain the coated perlite.

Preparation example 12: the coated perlite is prepared by the following steps:

1kg of carboxymethyl cellulose is weighed and dispersed into absolute ethyl alcohol with the mass fraction of 99 percent, then the absolute ethyl alcohol is added into 1kg of open-cell expanded perlite, the particle size of the carboxymethyl cellulose is 80nm, the particle size of the open-cell expanded perlite is 20 meshes, the ultrasonic dispersion is carried out for 10 minutes under the condition of 20kHz, and the loaded perlite is prepared after drying; weighing 0.35kg of ethyl cellulose solution, uniformly spraying the solution onto the surface of 1kg of load perlite, wherein the ethyl cellulose solution is ethyl cellulose solution with the mass fraction of 5%, the solvent of the ethyl cellulose solution is a mixed solvent of toluene/ethanol of 1/4, and drying to obtain the coated perlite.

Examples

The amino acid liquid in the following raw materials is purchased from Jinan blaze chemical industry Co., ltd; potassium fulvate is purchased from 370575, water-soluble potassium fulvate produced by city county scene source biotechnology limited; humic acid is purchased from Fengwang biotechnology limited company; seaweed chitin was purchased from Qingdao blue fairy crop nutrition limited; chitosan oligosaccharide is purchased from Qingdao Bozhi Hui biosciences Co., ltd; vitamins B12, B3 and C are purchased from Shandong Jiaoyang Biotechnology Co., ltd; trichoderma viride was purchased from Shandong Hezhong Kangyuan Biotechnology Co., ltd; bacillus subtilis is purchased from Jinan Xin Weida chemical industry Co., ltd; bacillus licheniformis was purchased from Jining Allida bioengineering Inc.; 5406 actinomycetes were purchased from Jining Alida bioengineering Co., ltd; sesbania gum was purchased from shandong duckweed poly biotechnology limited; other raw materials and equipment are all commonly and commercially available.

Example 1: an LTC nutrition chelation production process of a compound microbial fertilizer comprises the following steps:

s1, accurate nutrient proportioning: 3kg of medium trace elements, 5kg of amino acid liquid, 3kg of potassium fulvate, 5kg of humic acid, 20kg of macroelement powder, 15kg of plant extract, 4kg of seaweed chitin, 5kg of chitosan oligosaccharide, 0.8kg of vitamins and 160kg of sterile water; the medium trace element powder consists of calcium sulfate, magnesium sulfate and silicon dioxide in the weight ratio of 1:1:0.7; the macroelement powder consists of potassium phosphate, monopotassium phosphate, ammonium nitrate and ammonium sulfate in the weight ratio of 1:1.5:1:1.5; the plant extract consists of oleander extract prepared in preparation example 8, malachite extract prepared in preparation example 9 and sesbania gum in a weight ratio of 1:1.5:0.3; the vitamin is vitamin B12;

s2, nutrient is put into a tank: weighing medium trace element powder, fulvic acid, humic acid, macroelement powder and vitamin, adding into a liquid fermentation tank, adding plant extract, stirring uniformly, and finally adding amino acid liquid, seaweed chitin, chitosan oligosaccharide and water, mixing and stirring uniformly to obtain premix;

s3, sterilizing and cooling the nutrient at high temperature: sterilizing the premix prepared in the step S2 at 120 ℃ for 30min, and then cooling to 36 ℃ to prepare a mixed solution;

S4, weighing 0.8kg of composite strains, inoculating the composite strains into a liquid fermentation tank, uniformly mixing, contacting the composite strains with a mixed liquid, wherein the composite strains consist of trichoderma viride, bacillus subtilis, bacillus licheniformis and 5406 actinomycetes in a weight ratio of 1:2:1.2:0.8, and chelating the trichoderma viride, bacillus subtilis and bacillus licheniformis at a constant temperature of 36 ℃ for 120min, wherein the activity of microorganisms is detected every half hour in the chelating process, and the number of effective viable bacteria is not reduced to be qualified;

s5, filling and warehousing after the fertilizer is qualified to obtain the finished liquid fertilizer.

Example 2: an LTC nutrition chelation production process of a compound microbial fertilizer comprises the following steps:

s1, accurate nutrient proportioning: 1kg of medium trace elements, 3kg of amino acid liquid, 1kg of potassium fulvate, 2kg of humic acid, 15kg of macroelement powder, 10kg of plant extract, 2kg of seaweed chitin, 3kg of chitosan oligosaccharide and 0.5kg of vitamins; the medium trace element powder consists of calcium sulfate, magnesium sulfate and silicon dioxide in the weight ratio of 1:0.6:0.4; the macroelement powder consists of potassium phosphate, monopotassium phosphate, ammonium nitrate and ammonium sulfate in the weight ratio of 1:1:0.5:1; the plant extract consists of oleander extract prepared in preparation example 8, malachite extract prepared in preparation example 9 and sesbania gum in a weight ratio of 1:1:0.1; the vitamin is vitamin C;

s3, sterilizing and cooling the nutrient at high temperature: sterilizing the premix prepared in the step S2 at 115 ℃ for 35min, and then cooling to 32 ℃ to prepare a mixed solution;

s4, weighing 0.35kg of composite strains, inoculating the composite strains into a liquid fermentation tank, uniformly mixing, contacting the composite strains with a mixed liquid, wherein the composite strains consist of trichoderma viride, bacillus subtilis, bacillus licheniformis and 5406 actinomycetes in a weight ratio of 1:1:1:0.5, and chelating at a constant temperature of 36 ℃ for 100min, wherein the activity of microorganisms is detected every half hour in the chelating process, and the number of effective viable bacteria is not reduced to be qualified;

Example 3: an LTC nutrition chelation production process of a compound microbial fertilizer comprises the following steps:

s1, accurate nutrient proportioning: 5kg of medium trace elements, 8kg of amino acid liquid, 5kg of potassium fulvate, 7kg of humic acid, 25kg of macroelement powder, 20kg of plant extract, 6kg of seaweed chitin, 7kg of chitosan oligosaccharide and 1kg of vitamins; the medium trace element powder consists of calcium sulfate, magnesium sulfate and silicon dioxide in a weight ratio of 1:1.2:1; the macroelement powder consists of potassium phosphate, monopotassium phosphate, ammonium nitrate and ammonium sulfate in the weight ratio of 1:2:1.5:2; the plant extract consists of oleander extract prepared in preparation example 8, malachite extract prepared in preparation example 9 and sesbania gum in a weight ratio of 1:2:0.5; the vitamin is vitamin B3;

s3, sterilizing and cooling the nutrient at high temperature: sterilizing the premix prepared in the step S2 at 125 ℃ for 25min, and then cooling to 38 ℃ to prepare a mixed solution;

s4, weighing 1.3kg of composite strains, inoculating the composite strains into a liquid fermentation tank, uniformly mixing, and contacting the composite strains with a mixed liquid, wherein the composite strains consist of trichoderma viride, bacillus subtilis, bacillus licheniformis and 5406 actinomycetes in a weight ratio of 1:3:1.5:1; chelating at a constant temperature of 36 ℃ for 150min, detecting the activity of microorganisms every half an hour in the chelating process, and ensuring that the number of effective viable bacteria is not reduced to be qualified;

Example 4: this embodiment differs from embodiment 1 in that:

s1, accurate nutrient proportioning: adding 5kg of modified bamboo fiber prepared in preparation example 4 into the raw materials;

s2, nutrient is put into a tank: weighing medium trace element powder, fulvic acid, humic acid, macroelement powder and vitamin, adding into a liquid fermentation tank, adding plant extract, stirring uniformly, adding amino acid liquid, seaweed chitin, chitosan oligosaccharide and water, mixing and stirring uniformly, adding modified bamboo fiber, and stirring uniformly to obtain a premix.

Example 5: this embodiment differs from embodiment 1 in that:

s1, accurate nutrient proportioning: 3kg of the modified bamboo fiber prepared in preparation example 5 is added into the raw materials;

Example 6: this embodiment differs from embodiment 1 in that:

s1, accurate nutrient proportioning: adding 7kg of modified bamboo fiber prepared in preparation example 6 into the raw materials;

Example 7: this embodiment differs from embodiment 1 in that:

the same quality of bacillus subtilis is used for replacing trichoderma viride in the composite strain.

Example 8: this embodiment differs from embodiment 1 in that:

the 5406 actinomycetes are replaced by bacillus licheniformis with the same quality in the composite strain.

Example 9: this embodiment differs from embodiment 1 in that:

the compound strain is prepared by replacing 5406 actinomycetes with trichoderma viride of the same quality.

Example 10: this embodiment differs from embodiment 1 in that:

the bacillus subtilis with the same quality is used for replacing the bacillus licheniformis in the composite strain.

Example 11: this embodiment differs from embodiment 4 in that:

sesbania gum is not added into the plant extract raw material.

Example 12: this embodiment differs from embodiment 4 in that:

the modified bamboo fiber prepared in preparation example 7 is selected as the modified bamboo fiber.

Example 13: this embodiment differs from embodiment 4 in that:

the silicon dioxide in the raw materials for preparing the modified bamboo fiber is not modified.

Example 14: this embodiment differs from embodiment 4 in that:

s1, accurate nutrient proportioning: 4kg of the coated perlite prepared in preparation example 10 is added into the raw materials;

s2, nutrient is put into a tank: weighing medium trace element powder, fulvic acid, humic acid, macroelement powder and vitamins, adding into a liquid fermentation tank, adding plant extract, stirring uniformly, adding amino acid liquid, seaweed chitin, chitosan oligosaccharide and water, mixing and stirring uniformly, and finally adding modified bamboo fiber and coated perlite, and stirring uniformly to obtain a premix.

Example 15: this embodiment differs from embodiment 4 in that:

s1, accurate nutrient proportioning: 2kg of the coated perlite prepared in preparation example 11 is added into the raw materials;

Example 16: this embodiment differs from embodiment 4 in that:

s1, accurate nutrient proportioning: 6kg of the coated perlite prepared in preparation example 12 is added into the raw materials;

Example 17: this embodiment differs from embodiment 14 in that:

In the preparation process of the coated perlite, 0.75kg of carboxymethyl cellulose is weighed and dispersed into absolute ethyl alcohol, then 1kg of open-cell expanded perlite is added, the particle size of the carboxymethyl cellulose is 80nm, the particle size of the open-cell expanded perlite is 20 meshes, ultrasonic dispersion is carried out for 10min under the condition of 20kHz, and the coated perlite is obtained after drying.

Example 18: this embodiment differs from embodiment 14 in that:

in the preparation process of the coated perlite, 0.35kg of ethyl cellulose solution is weighed and evenly sprayed on the surface of 1kg of open-pore expanded perlite, the ethyl cellulose solution is ethyl cellulose solution with the mass fraction of 5%, the solvent of the ethyl cellulose solution is a mixed solvent of toluene/ethanol of 1/4, and the coated perlite is prepared after drying.

Comparative example

Comparative example 1: the comparative example is different from example 1 in that the raw materials are humic acid with the same quality to replace amino acid liquid and chitosan oligosaccharide.

Comparative example 2: this comparative example is different from example 1 in that no plant extract was added to the raw material.

Comparative example 3: this comparative example differs from example 1 in that no algal chitin was added to the raw materials.

Performance test

1. Corn yield test

The preparation methods of examples 1-18 and comparative examples 1-3 were used to prepare fertilizers, the land in the northeast Jilin area was selected, 50kg of fertilizer was applied per mu of land, corn was planted after the fertilizer application, and the yield of autumn-harvested corn was recorded as the yield of non-rainfall corn.

The preparation methods of examples 1-18 and comparative examples 1-3 were used to prepare fertilizers, 50kg of fertilizer was applied to the land in the northeast Jilin area, corn was planted, artificial rainfall was used for 20 hours after fertilization, 1 ton of water was used per mu of land, and the yield of autumn-harvested corn was recorded as the yield of rainfall corn.

2. Corn performance test

The preparation methods of examples 1-13 and comparative examples 1-3 were used to prepare fertilizers, 50kg of fertilizer was applied to each mu of land was selected for use in the northeast Jilin region, corn was planted after fertilization, corn plants were cut at a height position of 2.2m after autumn corn was ripe, the probability of corn plant height being 2.2m or more was calculated, and data were recorded.

The preparation methods of examples 1-13 and comparative examples 1-3 are respectively adopted to prepare fertilizers, 50kg of fertilizer is applied to the land in northeast Jilin areas, corn is planted after the fertilizer is applied, corn plant yellow She Qingkuang is scored when the corn grows to 1m, and the score of She Gailv-100% of each mu of rehmannia is respectively 10-0 minutes.

The fertilizer was prepared by the preparation methods of examples 1 to 13 and comparative examples 1 to 3, respectively, 50kg of fertilizer was applied per mu of land was selected for the land in the northeast Jilin region, corn was planted after the fertilizer application, the length of the corn root was measured after the corn was grown (the length of the root was perfect, and the damaged length of the corn root was not counted), and data was recorded.

3. Rice yield test

The preparation methods of examples 1-13 and comparative examples 1-3 are adopted to prepare fertilizers, 50kg of fertilizer is applied to the land in northeast Jilin areas, 20kg of phosphate fertilizer is added to the land after the fertilizer is applied, and the yield of autumn rice is recorded after the rice is planted to be about 20cm in height.

Table 1 fertilizer performance test table

As can be seen from the combination of examples 1-3 and examples 4-6 and Table 1, the modified bamboo fibers added to the raw materials of examples 4-6 were higher in corn yield than example 1 in both the non-rainfall and rainfall conditions of examples 4-6, and the probability of the corn plant height of 2.2m was higher than that of example 1, the yellow leaf score was higher than that of example 1, the corn root length was higher than that of example 1, and the rice yield of examples 4-6 was higher than that of example 1, as compared with examples 1-3; the modified bamboo fiber, humic acid and chitosan oligosaccharide are combined, and the binding effect of the modified cellulose on the chelate is utilized, so that nutrient elements in the prepared fertilizer can be slowly released, the vitality of microorganisms is improved, meanwhile, the phenomenon that substances which are not melted in the fertilizer are washed away by rainwater due to excessive rainfall is avoided, and the using effect of the fertilizer is affected, so that the planted crops are high in yield and quality is realized.

As can be seen from the combination of example 1 and examples 7 to 13 and the combination of table 1, in example 7, the composite strain material of example 7 replaced trichoderma viride with bacillus subtilis of the same quality, compared with example 1, the corn yield of example 7 under the condition of no rainfall and rainfall is lower than that of example 1, the probability that the corn plant height of example 7 is equal to or greater than 2.2m is lower than that of example 1, the yellow leaf score is lower than that of example 1, the corn root length is lower than that of example 1, and the rice yield of example 7 is lower than that of example 1; the trichoderma viride and the bacillus licheniformis are matched, so that a good protection effect can be achieved on the root system of crops, the effective root system length of the crops is high, nutrients, moisture and other substances in the soil can be better absorbed, the growth of the crops is promoted, and the planted crops are high in yield and quality.

Compared with example 1, the example 8 has the advantages that the 5406 actinomycetes are replaced by bacillus licheniformis with the same quality in the composite strain raw material, compared with example 1, the corn yield under the condition of no rainfall and rainfall is lower than that of example 1, the probability that the corn plant height of example 8 is more than or equal to 2.2m is lower than that of example 1, the yellow leaf score is lower than that of example 1, the corn root length is lower than that of example 1, and the rice yield of example 8 is lower than that of example 1; the bacillus licheniformis and 5406 actinomycetes are matched, and the organic matters in the soil are improved, and the crop root system is promoted to absorb the nutrient substances, so that the crops are high in yield and quality.

Compared with example 1, the example 9 has the advantages that the compound strain raw material is replaced by trichoderma viride of the same quality, compared with example 1, the example 9 has lower corn yield under the conditions of no rainfall and rainfall than example 1, the probability that the corn plant height of example 9 is more than or equal to 2.2m is lower than example 1, the yellow leaf score is lower than example 1, the corn root length is lower than example 1, and the rice yield of example 9 is lower than the rice yield of example 1; the 5406 actinomycetes and the bacillus subtilis are matched, so that nutrient substances such as chitin in soil can be promoted to decompose, and the bacillus subtilis can better absorb nutrients by a crop root system due to the inhibition effect of the bacillus subtilis on pathogenic bacteria, so that crops are high in yield and quality.

Compared with example 1, the example 10 has lower corn yield under the conditions of no rainfall and rainfall than example 1, and the example 10 has lower probability of corn plant height not less than 2.2m than example 1, yellow leaf score lower than example 1, corn root length lower than example 1, and example 10 rice yield lower than example 1; the bacillus subtilis, the trichoderma viride, the bacillus licheniformis and the 5406 actinomycetes are matched, so that the fertilizer has the advantages of preventing and treating various soil-borne diseases, killing pests, preventing and treating the pests, promoting the growth of crops, improving the stress resistance of the crops, improving the soil structure, degrading harmful substances in the soil, decomposing nitrogen, phosphorus and potassium, enhancing the fertilizer efficiency and the like, thereby promoting the growth of the crops and ensuring the high yield and the high quality of the crops.

Compared with example 4, the corn yield of example 11 under the condition of no rainfall and no rainfall is lower than that of example 4, wherein the corn yield under the rainfall condition is obviously reduced, which means that the bamboo fiber, the acrylamide, the sesbania gum and the modified silica are matched, the surface area of the bamboo fiber is improved by grafting the modified silica on the surface of the bamboo fiber, and the bonding effect of the modified bamboo fiber and the chelate is improved by matching with the better bonding effect of the acrylamide and the sesbania gum, so that the modified bamboo fiber better binds the chelate of microorganisms and energy elements, thereby avoiding that part of the fertilizer is washed away by rainwater without melting substances, and ensuring that crops are high in yield and quality.

In example 12, the modified bamboo fiber prepared in preparation example 7 is selected, and the bamboo fiber in preparation example 7 is not modified, compared with example 4, the corn yield under the rainfall condition of example 12 is lower than that of example 4, which means that the bamboo fiber, the acrylamide and the modified silica are matched, the surface area of the bamboo fiber is increased by modifying the surface of the bamboo fiber, the contact site between the bamboo fiber and the chelate is improved, the hydrophobic effect of the modified silica is matched by utilizing the larger gravity of the bamboo fiber after water absorption, the phenomenon that the rain water washes away substances which are not melted in the fertilizer is avoided, the better absorption of nutrient substances by crops is ensured, and the crops are high in yield and high quality.

Compared with example 4, the corn yield of the modified bamboo fiber raw material in example 13 is lower than that of example 4, which shows that the modified silica has better hydrophobic effect, the contact amount of rainwater and bamboo fibers is reduced by utilizing the hydrophobic effect, and the gravity is increased after the bamboo fibers absorb water, so that the modified bamboo fiber bonding chelate is fixed on the ground surface, the probability of flushing away by rainwater is reduced, and crops can absorb nutrient substances better, and the crops have high yield and high quality.

It can be seen in combination with examples 4 and examples 14-16 and with Table 1 that the addition of coated perlite prevents the removal of fertilizer by rainfall water, thereby further preserving the fertilizer content in the soil to promote crop growth and provide high crop yield.

As can be seen from the combination of examples 14 and examples 17-18 and table 1, the coated perlite of example 17 was not treated with ethylcellulose solution, and the coated perlite of example 18 was not treated with hydroxymethyl cellulose, and the fertilizers prepared in examples 17 and 18 were less resistant to rain wash than in example 14, as compared to example 1; the hydroxymethyl cellulose, the expanded perlite and the ethylcellulose solution are matched, so that the adhesiveness of the chelating strain can be improved, the chelating strain can be firmly adhered to the surfaces of the modified group fibers and the coated perlite, and raw material substances in the fertilizer are prevented from being taken away by rainwater as much as possible, so that the growth of crops is ensured.

As can be seen by combining examples 1-3 and comparative examples 1-3 and combining table 1, the comparative example 1 has the same mass of humic acid in the raw material to replace amino acid liquid and chitosan oligosaccharide, compared with example 1, the corn yield of comparative example 1 under the condition of no rainfall and rainfall is lower than that of example 1, the probability that the corn plant height of comparative example 1 is more than or equal to 2.2m is lower than that of example 1, the yellow leaf score is lower than that of example 1, the corn root length is lower than that of example 1, and the rice yield of comparative example 1 is lower than that of example 1; the amino acid liquid, the humic acid and the fulvic acid are matched, so that the saline-alkali resistance effect is achieved, the pH value of soil can be regulated, and the growth of root systems is promoted by building a good soil environment, so that the good growth of crops is ensured, the quality of the crops is improved, and the yield of the crops is promoted.

Compared with the example 1, the corn yield of the comparative example 2 under the conditions of no rainfall and no rainfall is lower than the example 1, the probability that the corn plant height of the comparative example 2 is more than or equal to 2.2m is lower than the example 1, the yellow leaf score is lower than the example 1, the corn root length is lower than the example 1, and the rice yield of the comparative example 2 is lower than the rice yield of the example 1; the plant extract, the composite strain and the chitosan oligosaccharide are matched, so that continuous cropping, nematode inhibition, drought resistance, cold resistance and dry heat resistance can be realized, the crop stress resistance is improved, the crop growth is prevented from being influenced by the external environment, the crop quality is improved, and the crop yield is increased.

Compared with the example 1, the corn yield of the comparative example 3 under the conditions of no rainfall and no rainfall is lower than the example 1, the probability that the corn plant height of the comparative example 3 is more than or equal to 2.2m is lower than the example 1, the yellow leaf score is lower than the example 1, the corn root length is lower than the example 1, and the rice yield of the comparative example 3 is lower than the rice yield of the example 1; the composite strain and seaweed chitin are matched, so that the effects of nourishing, protecting, increasing and strengthening roots, preventing and controlling red roots, black roots, rotten roots, dying plants and the like can be achieved, the root systems of crops are protected well, and nutrient substances are absorbed better, so that the quality of the crops is improved, and the yield of the crops is increased.

The present embodiment is merely illustrative of the present application and is not intended to be limiting, and those skilled in the art, after having read the present specification, may make modifications to the present embodiment without creative contribution as required, but is protected by patent laws within the scope of the claims of the present application.

Claims

1. The compound microbial fertilizer is characterized by comprising the following raw materials in parts by weight: 0.35-1.3 parts of composite strain, 1-5 parts of medium trace element powder, 3-8 parts of amino acid liquid, 1-5 parts of fulvic acid, 2-7 parts of humic acid, 15-25 parts of macroelement powder, 10-20 parts of plant extract, 2-6 parts of seaweed chitin, 3-7 parts of chitosan oligosaccharide, 0.5-1 part of vitamin and 100-200 parts of sterile water; the composite strain consists of trichoderma viride, bacillus subtilis, bacillus licheniformis and 5406 actinomycetes in a weight ratio of 1:1-3:1-1.5:0.5-1; the medium trace element powder consists of calcium sulfate, magnesium sulfate and silicon dioxide in a weight ratio of 1:0.6-1.2:0.4-1; the macroelement powder consists of potassium phosphate, monopotassium phosphate, ammonium nitrate and ammonium sulfate in a weight ratio of 1:1-2:0.5-1.5:1-2; the plant extract consists of oleander extract, malachite extract and sesbania gum in the weight ratio of 1:1-2:0.1-0.5; the fertilizer also comprises the following raw materials in parts by weight: 3-7 parts of modified bamboo fiber;

The modified bamboo fiber is prepared by the following method:

III, washing the semi-finished product prepared in the step II to be neutral, dehydrating by absolute ethyl alcohol, drying, crushing and vacuum drying to prepare modified bamboo fibers;

the modified silicon dioxide is prepared by the following method: weighing 20-35 parts of silicon dioxide, placing the silicon dioxide in 55-75 parts of fluorosilane solution, performing ultrasonic dispersion for 5-10min, taking out the silicon dioxide, and drying at room temperature to obtain modified silicon dioxide;

the preparation method of the compound microbial fertilizer comprises the following steps:

2. The compound microbial fertilizer according to claim 1, further comprising 2-6 parts of coated perlite, wherein the coated perlite is prepared by the following method:

weighing carboxymethyl cellulose, dispersing the carboxymethyl cellulose into the open-cell expanded perlite, wherein the weight ratio of the carboxymethyl cellulose to the open-cell expanded perlite is 0.5-1:1, and preparing the loaded perlite; and uniformly spraying an ethyl cellulose solution on the surface of the loaded perlite, wherein the mass ratio of the ethyl cellulose solution to the loaded perlite is 0.1-0.35:1, and drying to obtain the coated perlite.

3. A process for the production of a compound microbial fertilizer according to any one of claims 1-2.