CN114921372A - 一种提高鸡蛋品质的益生菌制剂、制备方法及应用 - Google Patents

一种提高鸡蛋品质的益生菌制剂、制备方法及应用 Download PDF

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CN114921372A
CN114921372A CN202210569209.4A CN202210569209A CN114921372A CN 114921372 A CN114921372 A CN 114921372A CN 202210569209 A CN202210569209 A CN 202210569209A CN 114921372 A CN114921372 A CN 114921372A
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improving
preparation
quality
probiotic
feed
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刘晏辅
武存芳
屈红军
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Shaanxi Future Green Agriculture Animal Husbandry Dev Co ltd
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Shaanxi Future Green Agriculture Animal Husbandry Dev Co ltd
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Abstract

本发明属于微生物菌剂技术领域，公开了一种提高鸡蛋品质的益生菌制剂、制备方法及应用，提高鸡蛋品质的益生菌制剂由复合菌种和复合酶制剂组成；复合菌种包括地衣芽孢杆菌、枯草芽孢杆菌、两歧双歧杆菌、乳酸菌、嗜酸乳杆菌、保加利亚乳杆菌、丁酸梭菌、植物乳杆菌、产朊假丝酵母、酿酒酵母和沼泽红假单胞菌；复合酶制剂包括淀粉酶、纤维素酶、β‑葡聚糖酶、葡萄糖氧化酶、脂肪酶、麦芽糖酶、甘露聚糖酶、果胶酶、植酸酶、蛋白酶、支链淀粉酶、木聚糖酶和半乳甘露聚糖酶。本发明的提高鸡蛋品质的益生菌制剂能够提高饲料消化吸收率、减少养殖栏舍中臭味氨气；降低苍蝇密度与鸡粪含水量，促进鸡粪发酵；促进生长与提高产蛋率，降低死亡淘汰率。

Description

一种提高鸡蛋品质的益生菌制剂、制备方法及应用
技术领域
本发明属于微生物菌剂技术领域，尤其涉及一种提高鸡蛋品质的益生菌制剂、制备方法及应用。
背景技术
目前，益生菌是一类对宿主有益的活性微生物，是定植于人体肠道、生殖系统内，能产生确切健康功效从而改善宿主微生态平衡、发挥有益作用的活性有益微生物的总称。人体、动物体内有益的细菌或真菌主要有：酪酸梭菌、乳杆菌、双歧杆菌、放线菌、酵母菌等。目前世界上研究的功能最强大的产品主要是以上各类微生物组成的复合活性益生菌，其广泛应用于生物工程、工农业、食品安全以及生命健康领域。
合理有效的益生菌制剂能够有效修复肠道菌群生态系统平衡，是促进人和动物机体保持健康生长状态的关键。固态型益生菌制剂具有易于保存、运输、应用等特点，目前关于商品化的动物益生菌制剂常为液态，传统的固态益生菌制剂中益生菌由于干燥极易死亡，且不能长期保存，从而丧失利用价值。
中国专利CN103690568A公开了一种利用高渗低聚糖液制备固态益生菌制剂的方法，包括以下步骤：1)量取体积为Vml的益生菌菌液，向益生菌菌液中加入保护剂混合均匀后，再加入低聚糖充分搅拌，得到益生菌混合液，所加入保护剂的质量为1～8％Vg，所加入的低聚糖质量为30～70％Vg；2)将益生菌混合液在20～42℃的条件下保温处理30～240min；3)向经保温处理的益生菌混合液中加入发泡膨松剂混合均匀后，再加入辅料充分搅拌，得到益生菌混合物料，所加入的发泡膨松剂质量为0.1～0.8％Vg，所加入辅料的质量为40～80％Vg；4)将益生菌混合物料在30～37℃下真空干燥，然后进行粉碎，即得到固态益生菌制剂。该工艺中使用低聚糖的渗透压效果使细胞脱水，容易导致细胞死亡，且其保护剂在冷冻干燥工艺中的保护效果较差，因此其实际应用效果欠佳。
中国专利CN104250630A公开了一种益生菌固态制剂的制备方法，包括：分别配制含琼脂LB培养基或含琼脂生理盐水，以及LB培养基或生理盐水，并高压灭菌；取益生菌高速离心沉淀，弃去上层菌液，用35～37℃预温的LB培养基或生理盐水重悬；把重悬的益生菌菌液接入液态含琼脂LB培养基或含琼脂生理盐水中，混匀；将液态包含益生菌的含琼脂的LB培养基或液态包含益生菌的含琼脂生理盐水分装至模具中，室温冷却，即凝固成具有模具形状的益生菌固态制剂。该制品的基质为LB培养基，其中的组分长期服用存在较大的安全隐患。
通过上述分析，现有技术存在的问题及缺陷为：
(1)传统的固态益生菌制剂中益生菌由于干燥极易死亡，且不能长期保存，从而丧失利用价值。
(2)现有技术使用低聚糖的渗透压效果使细胞脱水，容易导致细胞死亡，且其保护剂在冷冻干燥工艺中的保护效果较差，因此其实际应用效果欠佳。
(3)现有的益生菌制品的基质为LB培养基，其中的组分长期服用存在较大的安全隐患。
发明内容
针对现有技术存在的问题，本发明提供了一种提高鸡蛋品质的益生菌制剂、制备方法及应用。
本发明是这样实现的，一种提高鸡蛋品质的益生菌制剂，所述提高鸡蛋品质的益生菌制剂由复合菌种和复合酶制剂组成。
进一步，所述复合菌种包括地衣芽孢杆菌、枯草芽孢杆菌、两歧双歧杆菌、乳酸菌、嗜酸乳杆菌、保加利亚乳杆菌、丁酸梭菌、植物乳杆菌、产朊假丝酵母、酿酒酵母和沼泽红假单胞菌。
进一步，所述乳酸菌包括粪肠球菌和屎肠球菌。
进一步，所述复合菌种综合每克≥100亿cfu/g。
进一步，所述复合酶制剂包括淀粉酶、纤维素酶、β-葡聚糖酶、葡萄糖氧化酶、脂肪酶、麦芽糖酶、甘露聚糖酶、果胶酶、植酸酶、蛋白酶、支链淀粉酶、木聚糖酶和半乳甘露聚糖酶。
本发明的另一目的在于提供一种实施所述的提高鸡蛋品质的益生菌制剂的提高鸡蛋品质的益生菌制剂的使用方法。
进一步，所述提高鸡蛋品质的益生菌制剂的用量包括：
雏鸡为500克/吨饲料；中鸡为400克/吨饲料，大鸡为300克/吨饲料，蛋鸡为400克/吨饲料，饮水为100～200克/吨水。
进一步，所述提高鸡蛋品质的益生菌制剂的使用方法包括：
(1)混料饲喂方法：直接混入全价颗粒饲料或自配粉料中，或将益生菌制剂全部混合饲喂量10％的饲料中饲喂，其他90％的料直接投喂；
(2)饮水饲喂方法：将益生菌制剂等重红糖溶解在饮水中一小时后使用；
(3)制粒方法：采用多层包埋技术直接进行制粒，制粒中加大20％用量。
进一步，所述混料饲喂方法还包括将益生菌制剂与等重的红糖、10～20倍低于45℃的温水混合1小时以上，喷洒到饲料上并翻拌后进行饲喂。
进一步，所述饮水饲喂方法中，饮水器具使用前需清洗。
结合上述的技术方案和解决的技术问题，请从以下几方面分析本发明所要保护的技术方案所具备的优点及积极效果为：
第一、针对上述现有技术存在的技术问题以及解决该问题的难度，紧密结合本发明的所要保护的技术方案以及研发过程中结果和数据等，详细、深刻地分析本发明技术方案如何解决的技术问题，解决问题之后带来的一些具备创造性的技术效果。具体描述如下：
本发明提供的提高鸡蛋品质的益生菌制剂是专门针对鸡的生理特性而设计的复合益生菌，特别是针对鸡肠短的特点，采用速效益生菌结合复合酶制剂的技术，从而达到满意的使用效果。本品使用简单、适口性好，具有使用剂量小、成本低廉、安全、无毒，无副作用等特点。
本发明的主要作用如下：
1、节约饲料成本：本品在动物体内大量繁殖代谢，产生大量的淀粉酶、蛋白酶、脂肪酶等，能促进动物对糖类、蛋白质和脂肪的消化吸收，乳酸菌的分泌的乳酸等酸性物质使肠道环境呈酸性，也有利于营养素的消化吸收，因此本品能提高鸡群对饲料的消化吸收率，降低料耗，节约成本。
2、改善养殖生态环境：有效调节鸡的肠道菌群平衡，明显改善肠道微生态环境，抑制沙门氏菌、致病性大肠杆菌的生长和繁殖，从而降低粪便与养殖环境的氨氮味、臭味、异味，改善饲养环境。垫料中的致病菌量会明显下降，能够减少养殖环境及粪便中有机磷等有害物质的含量，减少污染。连续使用本品后，养殖场遍布有益菌，形成益生菌生态养殖环境，粪污水道与储水池能够自动分解改善水质，通过沼气或多级储水池过滤后结合如水生植物等措施能够达到合格排放；
3、减少鸡粪含水量，并消除鸡粪产生的氨氮。清理出来的鸡粪便能够自动快速发酵(含水量低于70％，注意薄膜覆盖密封)成生物菌肥，保护了生态环境。
4、保健效果明显：本品中的以乳酸菌为主的益生菌，鸡食用后能顺利通过胃酸及胆汁的考验，抵达肠道后能迅速增值，促进鸡肠道有益菌落如双歧杆菌、乳酸杆菌的生长，拮抗和抑制肠道内有害菌增殖，纠正肠道菌群紊乱，使鸡肠道菌群正常化，对鸡群肠道保健效果显著。使用本产品后能够明显提高雏鸡的成活率、减少肠道健康问题的发生，鸡群健壮整齐，毛色光顺漂亮。
5、增强饲料营养：本品在动物体内能产生多种有益代谢产物如有机酸、维生素、氨基酸等，直接补充动物生长所需营养，提高产蛋率。
6、改善肉、蛋品质：本品能加强饲料的适口性，降低血清胆固醇含量，减少肉蛋中的药品残留，改善鸡肉和蛋品质，减少破损率与坏蛋比例，长期使用蛋壳鲜亮光滑、肉蛋自然香醇，适合生产生态有机肉有机蛋。
7、节约抗生素等药品开支：长期在饲料中使用本产品，由于保健效果明显，可以减少或不使用抗生素，明显减少药品开支，提高鸡群的成活率，让养殖的鸡肉、鸡蛋更符合食品安全。
8、本品所用菌株稳定性好，抗逆性强，具有耐热、耐酸、耐胆汁、无污染、无任何违禁物质、绿色环保的特点，可长期添加。
第二，把技术方案看做一个整体或者从产品的角度，本发明所要保护的技术方案具备的技术效果和优点，具体描述如下：
本发明提供的提高鸡蛋品质的益生菌制剂，能够提高饲料消化吸收率、减少养殖栏舍中臭味氨气；降低苍蝇密度与鸡粪含水量，促进鸡粪发酵；提高肠道健康水平，改善肉蛋品质；促进生长与提高产蛋率，降低死亡淘汰率。
附图说明
为了更清楚地说明本发明实施例的技术方案，下面将对本发明实施例中所需要使用的附图做简单的介绍，显而易见地，下面所描述的附图仅仅是本发明的一些实施例，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下还可以根据这些附图获得其他的附图。
图1是本发明实施例提供的提高鸡蛋品质的益生菌制剂的使用方法流程图。
具体实施方式
为了使本发明的目的、技术方案及优点更加清楚明白，以下结合实施例，对本发明进行进一步详细说明。应当理解，此处所描述的具体实施例仅仅用以解释本发明，并不用于限定本发明。
针对现有技术存在的问题，本发明提供了一种提高鸡蛋品质的益生菌制剂、制备方法及应用，下面结合附图对本发明作详细的描述。
一、解释说明实施例。为了使本领域技术人员充分了解本发明如何具体实现，该部分是对权利要求技术方案进行展开说明的解释说明实施例。
本发明实施例提供的提高鸡蛋品质的益生菌制剂由复合菌种和复合酶制剂组成；其中，复合菌种包括地衣芽孢杆菌、枯草芽孢杆菌、两歧双歧杆菌、乳酸菌、嗜酸乳杆菌、保加利亚乳杆菌、丁酸梭菌、植物乳杆菌、产朊假丝酵母、酿酒酵母和沼泽红假单胞菌；复合酶制剂包括淀粉酶、纤维素酶、β-葡聚糖酶、葡萄糖氧化酶、脂肪酶、麦芽糖酶、甘露聚糖酶、果胶酶、植酸酶、蛋白酶、支链淀粉酶、木聚糖酶和半乳甘露聚糖酶。
本发明实施例提供的乳酸菌包括粪肠球菌和屎肠球菌。
本发明实施例提供的复合菌种综合每克≥100亿cfu/g。
本发明实施例提供的提高鸡蛋品质的益生菌制剂的用量包括：
雏鸡为500克/吨饲料；中鸡为400克/吨饲料，大鸡为300克/吨饲料，蛋鸡为400克/吨饲料，饮水为100～200克/吨水。
如图1所示，本发明实施例提供的提高鸡蛋品质的益生菌制剂的使用方法包括：
S101，混料饲喂方法：直接混入全价颗粒饲料或自配粉料中，或将益生菌制剂全部混合饲喂量10％的饲料中饲喂，其他90％的料直接投喂；
S102，饮水饲喂方法：将益生菌制剂等重红糖溶解在饮水中一小时后使用；
S103，制粒方法：采用多层包埋技术直接进行制粒，制粒中加大20％用量。
本发明实施例提供的混料饲喂方法还包括将益生菌制剂与等重的红糖、10～20倍低于45℃的温水混合1小时以上，喷洒到饲料上并翻拌后进行饲喂。
本发明实施例提供的饮水饲喂方法中，饮水器具使用前需清洗。
二、应用实施例。为了证明本发明的技术方案的创造性和技术价值，该部分是对权利要求技术方案进行具体产品上或相关技术上的应用实施例。
本发明实施例提供的提高鸡蛋品质的益生菌制剂包括：
主要组成菌种：地衣芽孢杆菌、枯草芽孢杆菌、两歧双歧杆菌、乳酸菌(粪肠球菌、屎肠球菌)、嗜酸乳杆菌、保加利亚乳杆菌、丁酸梭菌、植物乳杆菌、产朊假丝酵母、酿酒酵母、沼泽红假单胞菌，综合每克≥100亿cfu/g。
主要组成酶制剂：淀粉酶、纤维素酶、β-葡聚糖酶、葡萄糖氧化酶、脂肪酶、麦芽糖酶、甘露聚糖酶、果胶酶、植酸酶、蛋白酶、支链淀粉酶、木聚糖酶、半乳甘露聚糖酶。
本发明实施例提供的提高鸡蛋品质的益生菌制剂用法用量如下：
饲养阶段用量(克/吨饲料)：
雏鸡500；中鸡400
大鸡300；蛋鸡400
饮水100～200克/吨水
开始使用请按照上述使用量的一倍连续使用一周，之后按照上述用量，能够快速看到效果。
1、混料饲喂方法：直接混入全价颗粒饲料或自配粉料中，也可以将本品全部混合饲喂量10％的饲料中先饲喂，其他90％的料直接投喂即可。或将本品与等重的红糖、10～20倍低于45℃的温水混合1小时以上，喷洒到饲料上简单翻拌一下进行饲喂，效果更显著。
2、饮水饲喂方法：最好是与本品等重红糖一起溶解在饮水中一小时后再给鸡饮用。饮水器具建议经常清洗。
3、制粒方法：可以直接用于制粒，本品采用多层包埋技术，制粒中高温会带来部分损失，但只要加大20％的用量即可。
本发明实施例提供的提高鸡蛋品质的益生菌制剂无毒无害，饲料中预防和促长保健作用的抗生素不会影响使用效果，在大剂量抗生素治疗期间可能造成效果下降，但无副作用；部分种鸡蛋鸡在开始使用益生菌产品拌料饲喂时可能出现轻度拉稀等，这是正常现象，是由于之前抗生素使用频繁肠道紊乱，益生菌进入后需要几天的“斗争”期。
本发明实施例提供的提高鸡蛋品质的益生菌制剂可以与防霉剂一起使用，还可以减少或代替防霉剂。开封后尽快用完，未用完需要扎口保存。
本发明实施例提供的提高鸡蛋品质的益生菌制剂能够提高饲料消化吸收率、减少养殖栏舍中臭味氨气；降低苍蝇密度与鸡粪含水量，促进鸡粪发酵；提高肠道健康水平，改善肉蛋品质；促进生长与提高产蛋率，降低死亡淘汰率。
以上所述，仅为本发明的具体实施方式，但本发明的保护范围并不局限于此，任何熟悉本技术领域的技术人员在本发明揭露的技术范围内，凡在本发明的精神和原则之内所作的任何修改、等同替换和改进等，都应涵盖在本发明的保护范围之内。

Claims (10)

1.一种提高鸡蛋品质的益生菌制剂，其特征在于，所述提高鸡蛋品质的益生菌制剂由复合菌种和复合酶制剂组成。
2.如权利要求1所述提高鸡蛋品质的益生菌制剂，其特征在于，所述复合菌种包括地衣芽孢杆菌、枯草芽孢杆菌、两歧双歧杆菌、乳酸菌、嗜酸乳杆菌、保加利亚乳杆菌、丁酸梭菌、植物乳杆菌、产朊假丝酵母、酿酒酵母和沼泽红假单胞菌。
3.如权利要求2所述提高鸡蛋品质的益生菌制剂，其特征在于，所述乳酸菌包括粪肠球菌和屎肠球菌。
4.如权利要求1所述提高鸡蛋品质的益生菌制剂，其特征在于，所述复合菌种综合每克≥100亿cfu/g。
5.如权利要求1所述提高鸡蛋品质的益生菌制剂，其特征在于，所述复合酶制剂包括淀粉酶、纤维素酶、β-葡聚糖酶、葡萄糖氧化酶、脂肪酶、麦芽糖酶、甘露聚糖酶、果胶酶、植酸酶、蛋白酶、支链淀粉酶、木聚糖酶和半乳甘露聚糖酶。
6.一种实施如权利要求1～5任意一项所述提高鸡蛋品质的益生菌制剂的提高鸡蛋品质的益生菌制剂的使用方法。
7.如权利要求6所述提高鸡蛋品质的益生菌制剂的使用方法，其特征在于，所述提高鸡蛋品质的益生菌制剂的用量包括：
雏鸡为500克/吨饲料；中鸡为400克/吨饲料，大鸡为300克/吨饲料，蛋鸡为400克/吨饲料，饮水为100～200克/吨水。
8.如权利要求6所述提高鸡蛋品质的益生菌制剂的使用方法，其特征在于，所述提高鸡蛋品质的益生菌制剂的使用方法包括：
(1)混料饲喂方法：直接混入全价颗粒饲料或自配粉料中，或将益生菌制剂全部混合饲喂量10％的饲料中饲喂，其他90％的料直接投喂；
(2)饮水饲喂方法：将益生菌制剂等重红糖溶解在饮水中一小时后使用；
(3)制粒方法：采用多层包埋技术直接进行制粒，制粒中加大20％用量。
9.如权利要求8所述提高鸡蛋品质的益生菌制剂的使用方法，其特征在于，所述混料饲喂方法还包括将益生菌制剂与等重的红糖、10～20倍低于45℃的温水混合1小时以上，喷洒到饲料上并翻拌后进行饲喂。
10.如权利要求8所述提高鸡蛋品质的益生菌制剂的使用方法，其特征在于，所述饮水饲喂方法中，饮水器具使用前需清洗。
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