CN114920363A - Novel biological enzyme algaecide for river water treatment and preparation method thereof - Google Patents
Novel biological enzyme algaecide for river water treatment and preparation method thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/105—Phosphorus compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/007—Contaminated open waterways, rivers, lakes or ponds
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
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- Y02W10/37—Wastewater or sewage treatment systems using renewable energies using solar energy
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Abstract
The invention discloses a novel biological enzyme algaecide for river water treatment and a preparation method thereof, wherein the preparation method comprises the following steps: 1) adding enzyme into a proper amount of water to prepare an enzyme solution, and adding the prepared enzyme solution into a sodium alginate solution to obtain a mixed solution A; 2) adding the sterilized modified zeolite into the microbial bacterium mixed suspension D, oscillating for adsorption, and standing for a period of time to obtain a bacterium liquid mixture B; 3) adding the bacterial liquid mixture B obtained in the step 2) into the mixed liquid A obtained in the step 1), and uniformly mixing to obtain a mixed liquid C; 4) and adding a calcium chloride solution into the mixed solution C, carrying out a crosslinking reaction, and washing with sterile water after the crosslinking reaction is finished to obtain the novel biological enzyme algicide. The solid particles obtained by the method have better material stability and reuse rate, the integrity of a target water area can be protected to a great extent, no secondary pollution is caused, and the microorganisms and the biological enzymes act together, so that the method has a certain water quality improvement effect and shows excellent comprehensive treatment capacity.
Description
Technical Field
The invention relates to the technical field of sewage treatment, in particular to a novel biological enzyme algaecide for river water treatment and a preparation method thereof.
Background
Due to the leakage and drainage of various industrial and agricultural production wastewater and domestic sewage, mixed flow of rain and sewage, damage and extravasation of sewage pipe networks, poor river fluidity and the like, the problems of water eutrophication, water body black and stink and algae bloom are increasingly serious. The eutrophication of the water body means that the content of elements such as nitrogen and phosphorus in the water body is too high, and the direct adverse effect of the eutrophication is that the algae in the water body grow explosively, which is not beneficial to the local ecological protection, economic development and public health. The mass propagation of algae will occupy the living space of other aquatic organisms, so that the oxygen content in the water body is continuously reduced, the organisms in the water body die, and the water body is polluted and the water quality is deteriorated. Part of the algae can release algal toxins to further deteriorate the water pollution degree, and the human health is directly harmed. Therefore, the algae treatment of the water body is not slow enough.
In recent years, a great number of technical means have been developed by many scholars at home and abroad to reduce or remove algae in water bodies and further control algal bloom. The treatment of algae pollution in water body can be divided into three major categories, namely physical method, chemical method and biological method.
The physical method is to directly remove algae by using a physical principle, and the common physical methods comprise ultraviolet radiation algae removal, microfiltration algae removal, air floatation algae removal, ultrasonic algae removal and the like. The physical algae-removing method has obvious algae-removing effect, does not produce secondary pollution, but treats the symptoms and the root causes, and can only be used as a measure for treating algae outbreak.
Chemical algae removal is mainly to add chemical agents to hinder the growth and the propagation of algae so as to achieve the aim of removing algae; the chemical algae removal cost is relatively low, the application range is wide, the operation is simple and convenient, and the effect is obvious; the chemical algicide can rapidly, efficiently and thoroughly kill algae cells, but has obvious side effects and is easy to generate secondary pollution, and meanwhile, chemical medicines are enriched and amplified through a food chain to influence the healthy development of an ecological system, and in addition, the chemical medicines with low concentration can generate drug resistance for the algae after long-term use.
Biological algae removal is to degrade pollutants in water by using biological life activities so as to improve the water environment; biological algae removal has become a relatively good trend, and the method is simple to operate, free of secondary pollution, wide in treatment range, durable in effect, certain in selectivity and extremely low in damage degree to the ecological environment; but also has the limitation that the time is long, the monitoring is needed to be carried out all the time and corresponding adjustment is needed, and the biological disaster to the local species can be caused by introducing foreign organisms due to improper supervision.
In conclusion, although the traditional algaecide has a good treatment effect, the traditional algaecide also has obvious side effects, only treats the algae in the water body, does not have a further water quality purification effect, does not conform to the green development concept in river treatment, and urgently needs to develop a green and efficient algaecide.
Disclosure of Invention
Based on the defects of the prior art, the technical problem solved by the invention is to overcome the defects in the existing technology of river channel algae removal, and provide a novel biological enzyme algae removal agent for river channel water body treatment, which can protect the integrity of a target water area to the maximum extent, is efficient, specific, nontoxic, harmless, reusable, simple to operate, low in cost and wide in application range.
In order to solve the technical problems, the invention provides a preparation method of a novel biological enzyme algaecide for river water body treatment, which comprises the following steps:
(1) adding enzyme into a proper amount of water to prepare an enzyme solution, and adding the prepared enzyme solution into a sodium alginate solution to obtain a mixed solution A;
(2) adding the sterilized modified zeolite into the microbial bacterium mixed suspension D, oscillating for adsorption, and standing for a period of time to obtain a bacterium liquid mixture B;
(3) adding the bacterial liquid mixture B obtained in the step (2) into the mixed liquid A obtained in the step (1), and uniformly mixing to obtain a mixed liquid C;
(4) and (3) adding a calcium chloride solution into the mixed solution C to perform a crosslinking reaction, and washing with sterile water after the crosslinking reaction is finished to obtain the novel biological enzyme algaecide.
As the optimization of the technical scheme, the preparation method of the novel biological enzyme algaecide for river water body treatment further comprises part or all of the following technical characteristics:
as an improvement of the technical scheme, the novel biological enzyme algaecide comprises the following raw materials in parts by weight:
10-15 parts of modified zeolite;
15-25 parts of mixed bacteria;
30-40 parts of cellulase;
10-20 parts of pectinase;
the mixed bacteria comprise at least 4 of slime bacteria, Bacillus subtilis, yeast, phosphorus-accumulating bacteria, and ammonia nitrogen bacteria.
As an improvement of the technical scheme, the enzyme solution in the step (1) is formed by mixing at least two biological enzymes of pectinase and cellulase, and the mass concentration of the sodium alginate solution is 3-4%.
As an improvement of the technical scheme, the modified zeolite in the step (2) is an acid-modified zeolite with 200 meshes; the sterilization temperature is 121 ℃, and the sterilization time is 15 min; the oscillation speed of the oscillation adsorption is 150r/min, and the oscillation time is 30 min; the bacterial liquid mixture B mainly comprises a viscous bacterial group, a bacillus subtilis group, a yeast group, a phosphorus-accumulating group and an ammonia nitrogen group; the standing time is 24 hours, and the standing temperature is 25 ℃.
As an improvement of the technical scheme, the mass concentration of the calcium chloride solution in the step (4) is 2-4%, and the cross-linking reaction is carried out by placing the mixed solution C in a refrigerator at 4 ℃ for 24 hours.
The novel biological enzyme algaecide for the river water body treatment is prepared by any one of the methods.
The use method of the novel biological enzyme algaecide for river water body treatment comprises the following steps: the novel biological enzyme algaecide is filled into a medicine bag and then is put into a fixed position in a polluted river channel, and the adding amount of the novel biological enzyme algaecide is 500-1000 g/m 2 。
The recovery method of the novel biological enzyme algaecide for the river water body treatment comprises the following steps: the novel biological enzyme algaecide for treating the river water body after sewage treatment is washed by sterile water for 3 times or more, and can be repeatedly used.
As an improvement of the technical proposal, the repeatedly used novel biological enzyme algaecide has the algae removal rate of more than 70 percent, the ammonia nitrogen and total phosphorus removal rate of more than 60 percent and good stability of repeated use
The technical principle is as follows: pectinase is a complex enzyme and can decompose pectic substances in the cell wall of the algae so as to destroy the structure of the cell wall of the algae; cellulase is also a mixed enzyme, and various components in the cellulase synergistically degrade cellulose and have the function of hydrolyzing the cell wall of algae.
The combined use of pectinase and cellulase can also destroy the algal cell wall. After being treated by pectinase, the cell wall of the algae is damaged, so that the physicochemical factors such as osmotic pressure, potential and the like in the algae cell are changed, the normal physiology of the cell is influenced, and the growth of the cell is inhibited. Cellulases also have inhibitory effects on the growth of algal cells. The combination of pectinase and cellulase can also damage the glial sheath and cell wall of algal cells, which leads to the difficulty in maintaining normal life when being disturbed by external environment and finally leads to cell death.
The mixed microbial inoculum adopted by the invention is selected from at least 3 of slime bacteria flora, bacillus subtilis flora, yeast flora, phosphorus-accumulating flora and ammonia nitrogen flora. Wherein, the added slime bacteria in the mixed bacteria can secrete pectinase, which can provide pectinase, reduce the use of pectinase in the novel biological enzyme algaecide, and make up for the insufficient activity of pectinase at the later stage; the bacillus subtilis group in the mixed bacteria can secrete cellulase, so that the cellulase can be provided, the use of the cellulase in the novel biological enzyme algicide can be reduced, and the defect of insufficient activity of the cellulase in the later period of use can be made up; the yeast flora and the phosphorus-accumulating flora in the mixed bacteria are added, so that the content of organic matters and total phosphorus in the water body can be effectively reduced, the water quality is improved to a certain extent, the nutrients of algae can be effectively reduced, and the wild growth of the algae can be effectively prevented; the ammonia nitrogen flora in the mixed bacteria is added, so that the total nitrogen content in the water body can be effectively reduced, the water quality is improved to a certain degree, the nutrients of the algae can be effectively reduced, and the wild growth of the algae can be effectively prevented.
The foregoing description is only an overview of the technical solutions of the present invention, and in order to make the technical means of the present invention more clearly understood, the present invention may be implemented in accordance with the contents of the description, and in order to make the above and other objects, features, and advantages of the present invention more clearly understood, the following detailed description is given in conjunction with the preferred embodiments.
Detailed Description
Other aspects, features and advantages of the present invention will become apparent from the following detailed description, which, when taken in conjunction with the drawings, illustrate by way of example the principles of the invention.
Example 1
A preparation method of a novel biological enzyme algaecide for river water treatment specifically comprises the following steps:
(1) adding mixed enzyme such as pectinase, cellulose and the like into a proper amount of water to prepare mixed enzyme liquid, and adding the prepared mixed enzyme liquid into a sodium alginate solution to obtain a mixed solution A;
(2) adding the sterilized modified zeolite into the microbial bacterium mixed suspension D, oscillating for adsorption, and standing for a period of time to obtain a bacterium liquid mixture B;
(3) adding the bacterial liquid mixture B obtained in the step (2) into the mixed liquid A obtained in the step (1), and uniformly mixing to obtain a mixed liquid C;
(4) and (3) adding a calcium chloride solution into the mixed solution C to perform a crosslinking reaction, and washing with sterile water after the crosslinking reaction is finished to obtain the novel biological enzyme algaecide.
Comparative example 1
This comparative example differs from example 1 in that: the procedure was the same as in example 1 except that no cellulase was added.
Comparative example 2
This comparative example differs from example 1 in that: the procedure was the same as in example 1 except that no pectinase was added.
Comparative example 3
This comparative example differs from example 1 in that: the same procedure as in example 1 was repeated except that the bacterial liquid mixture was not added.
Comparative example 4
The comparative example differs from example 1 in that: the procedure of example 1 was repeated except that the mixed enzyme solution was not added.
Comparative example 5
The comparative example differs from example 1 in that: the same procedure as in example 1 was repeated except that the enzyme mixture and the bacterial suspension mixture were not added.
Under the laboratory conditions, 2.5g of novel biological enzyme algaecide immobilized particles for river water body treatment are used for treating water bodies with chlorophyll concentration of 118 mu g/L, ammonia nitrogen concentration of 14.10mg/L and total phosphorus concentration of 2.74mg/L, an equal amount and a proper amount of the novel biological enzyme algaecide after immobilization are sequentially added into each group of water bodies, and the chlorophyll concentration, the ammonia nitrogen concentration and the total phosphorus concentration in water are measured after oscillation reaction for 48 hours in a constant temperature oscillator at the temperature of 30 ℃ at 150r/min, so that the removal effects of the algaecide products of the embodiment 1 and the comparative examples 1, 2, 3, 4 and 5 are tested, and the results are shown in Table 1.
TABLE 1 removal Effect of Components of novel biological enzyme algaecide
| Examples | Chlorophyll removal rate (%) | Ammonia nitrogen removal (%) | Total phosphorus removal (%) |
| Example 1 | 96.61 | 85.10 | 83.94 |
| Comparative example 1 | 71.18 | 84.39 | 80.65 |
| Comparative example 2 | 73.73 | 84.75 | 82.11 |
| Comparative example 3 | 82.20 | 38.29 | 31.02 |
| Comparative example 4 | 43.22 | 84.18 | 78.46 |
| Comparative example 5 | 0.85 | 40.07 | 30.66 |
As can be seen from Table 1, compared with the algaecides in comparative examples 1 and 2, the mixed enzyme combination in the examples has better effect on removing algae in water, while the single enzyme solution in the comparative examples has poorer effect on removing algae; compared with the algaecide in the comparative example 3, the algaecide without the mixed bacterial liquid in the embodiment 1 of the invention has poorer water quality improvement effect; compared with the algaecide in the comparative example 4, although the mixed enzyme solution of pectinase, cellulase and the like is not contained, the algaecide also has a certain removal rate to algae, which shows that microorganisms in the mixed bacteria solution can secrete the mixed enzyme of pectinase, cellulase and the like, and can provide biological enzyme with high activity for the later treatment stage.
The different proportions of the various bacterial communities in the mixed bacterial liquid are different from the solutions with different algae removal effects, and the following are examples and comparative examples with different proportions
Example 2
This example differs from example 1 in that: the mass ratio of the slime bacteria colony, the bacillus subtilis colony, the yeast colony, the phosphorus-accumulating colony and the ammonia nitrogen colony in the mixed colony is 1: 3: 2: 2: 3, the other steps are the same as in example 1.
Example 3
This example differs from example 1 in that: the mass ratio of the slime bacteria colony, the bacillus subtilis colony, the yeast colony, the phosphorus-accumulating colony and the ammonia nitrogen colony in the mixed colony is 1: 1: 1: 1: 1, the other steps are the same as in example 1.
Example 4
This example differs from example 1 in that: the mass ratio of the slime bacteria colony, the bacillus subtilis colony, the yeast colony, the phosphorus-accumulating colony and the ammonia nitrogen colony in the mixed colony is 2: 1: 2: 2: 3, the other steps are the same as in example 1.
Example 5
The present example differs from example 1 in that: the mass ratio of the slime bacteria colony, the bacillus subtilis colony, the yeast colony, the phosphorus-accumulating colony and the ammonia nitrogen colony in the mixed colony is 1: 2: 2: 2: 3, the other steps are the same as in example 1.
Example 6
This example differs from example 1 in that: the mass ratio of the slime bacteria colony, the bacillus subtilis colony, the yeast colony, the phosphorus-accumulating colony and the ammonia nitrogen colony in the mixed colony is 1: 4: 2: 2: 3, the other steps are the same as in example 1.
Example 7
This example differs from example 1 in that: the mass ratio of the slime bacteria colony, the bacillus subtilis colony, the yeast colony, the phosphorus-accumulating colony and the ammonia nitrogen colony in the mixed colony is 1: 5: 2: 2: 3, the other steps are the same as in example 1.
Comparative example 6
This example differs from example 1 in that: no sticky bacteria group exists, and the mass ratio of the bacillus subtilis group, the yeast group, the phosphorus-accumulating group and the ammonia nitrogen group is 3: 2: 2: 3, the other steps are the same as in example 1.
Comparative example 7
This example differs from example 1 in that: the bacillus subtilis group is absent, and the mass ratio of the slime bacteria group, the yeast bacteria group, the phosphorus-accumulating bacteria group and the ammonia nitrogen bacteria group is 1: 2: 2: 3, the other steps are the same as in example 1.
Comparative example 8
This example differs from example 1 in that: the microbial agent does not contain yeast flora and phosphorus-accumulating flora, and the mass ratio of slime flora to bacillus subtilis to phosphorus-accumulating flora to ammonia nitrogen flora is 1: 3: 2: 3, the other steps are the same as in example 1.
Comparative example 9
This example differs from example 1 in that: no phosphorus-accumulating flora exists, and the mass ratio of the slime flora to the bacillus subtilis to the yeast flora to the ammonia nitrogen flora is 1: 3: 2: 3, the other steps are the same as in example 1.
Comparative example 10
The present example differs from example 1 in that: no ammonia nitrogen flora exists, and the mass ratio of the slime flora to the bacillus subtilis to the yeast flora to the phosphorus accumulating flora is 1: 3: 2: the other steps are the same as in example 1.
Under the laboratory conditions, 2.5g of novel biological enzyme algaecide immobilized particles for river water treatment are used for treating water with chlorophyll concentration of 118 mu g/L, ammonia nitrogen concentration of 14.10mg/L and total phosphorus concentration of 2.74mg/L, an equal amount and a proper amount of the novel biological enzyme algaecide immobilized particles are sequentially added into each group of water, and the chlorophyll concentration, the ammonia nitrogen concentration and the total phosphorus concentration in water are measured after oscillation reaction is carried out for 48 hours in a constant temperature oscillator at the temperature of 30 ℃ at 150r/min, so that the removal effects of different proportions of floras in the mixed bacterial liquid are tested, and the results are shown in Table 2.
TABLE 2 comparison of the removal effects of the novel bio-enzyme algaecide with different flora ratios
| Examples | Chlorophyll removal rate (%) | Ammonia nitrogen removal (%) | Total phosphorus removal (%) |
| Example 2 | 97.45 | 88.65 | 89.26 |
| Example 3 | 86.44 | 69.50 | 74.07 |
| Example 4 | 77.12 | 73.05 | 81.48 |
| Example 5 | 90.68 | 83.69 | 87.04 |
| Example 6 | 94.07 | 80.85 | 85.93 |
| Example 7 | 89.83 | 78.72 | 84.07 |
| Comparative example 6 | 83.05 | 82.62 | 85.40 |
| Comparative example 7 | 83.89 | 83.90 | 84.31 |
| Comparative example 8 | 91.53 | 84.83 | 71.89 |
| Comparative example 9 | 89.83 | 84.61 | 67.88 |
| Comparative example 10 | 88.98 | 46.31 | 85.04 |
As can be seen from table 2, when the mass ratio of the slime bacteria, the bacillus subtilis, the yeast bacteria, the phosphorus-accumulating bacteria, and the ammonia nitrogen bacteria in the mixed bacteria is 1: 3: 2: 2: 3, the effect of removing algae, ammonia nitrogen and total phosphorus in the water body is best. The comparison effect of the example 2, the comparative example 6 and the comparative example 7 shows that the slime bacteria and the bacillus subtilis in the mixed bacteria liquid have great influence on the removal of algae in the water body; the comparison effect of the embodiment 2 and the comparison examples 8 and 9 shows that the yeast flora and the phosphorus-accumulating flora in the mixed bacterial liquid have great influence on the removal of the total phosphorus in the water body; the comparison effect between the example 2 and the comparative example 10 shows that the yeast flora and the phosphorus-accumulating flora in the mixed bacterial liquid have great influence on the removal of ammonia nitrogen in the water body.
And (2) cleaning the water body biological enzyme algaecide for 3 times by using sterile water, then respectively putting the water bodies with the concentration of 118 mu g/L, the ammonia nitrogen concentration of 14.10mg/L and the total phosphorus concentration of 2.74mg/L into the water bodies for treatment, sequentially adding an equal amount of fixed novel biological enzyme algaecide into each group of water bodies, carrying out oscillation reaction in a constant-temperature oscillator at the temperature of 30 ℃ for 48 hours at the speed of 150r/min, then measuring the chlorophyll concentration, the ammonia nitrogen concentration and the total phosphorus concentration in the water, and repeating the steps for 3 times to test the reusability of the biological enzyme algaecide for the water body, wherein the test results are shown in Table 3.
TABLE 3 novel bioenzyme algaecide repeat Performance test
As can be seen from Table 3, the novel bio-enzyme algaecide still has a certain removal rate for algae, ammonia nitrogen and total phosphorus in the water body after being recycled for several times, the removal rate for algae is over 70% in the embodiment 2, the removal rate for ammonia nitrogen and total phosphorus is over 60%, and the stability of the repeated use of the algaecide is ensured.
1. The innovation points are as follows:
(1) according to the invention, mixed enzymes such as cellulase and pectinase are used to act on algae biological cells such as blue algae, the colloidal sheath and cell wall of the cells are damaged, the integrity of the cell structure is damaged, the normal life of the cells is difficult to maintain when the cells are interfered by the external environment, and finally the cells are dead. Can effectively remove the algae in the water body and has no secondary pollution.
(2) The biological enzyme used in the invention has specificity, only acts on algae organisms, does not act on other organisms, does not cause irreparable damage to the water body, does not need detoxification after use, does not need oxygenation, is simple and convenient to operate, is easy to implement, does not cause harm to fish, shrimp, crab and other organisms in the water body, and can protect the integrity of a target water area to the greatest extent.
(3) The viscous bacteria in the mixed microbial inoculum can secrete pectinase, the bacillus subtilis can secrete cellulase, the use of the biological enzyme in the novel biological enzyme algaecide can be reduced, the deficiency of the activity of the biological enzyme at the later stage of use can be made up, and the service cycle of the novel biological enzyme is prolonged.
(4) The yeast flora and the phosphorus-accumulating flora in the mixed microbial inoculum can effectively reduce the content of organic matters and total phosphorus in a water body, and the ammonia nitrogen flora can effectively reduce the content of total nitrogen in the water body, so that the water quality is improved to a certain extent, the nutrients of algae can be effectively reduced, and the wild growth of the algae can be effectively prevented.
(5) The invention has the advantages of easily obtained materials, simple preparation method, short production time, low cost, wide application range and certain water quality improvement effect. The biological enzyme also has multiple excellent characteristics of high efficiency, specificity, no toxicity, harmlessness, reusability and the like.
The materials listed in the invention, the upper and lower limits and interval values of the materials in the invention, and the upper and lower limits and interval values of the process parameters (such as temperature, time and the like) can all realize the invention, and the examples are not listed.
While the foregoing is directed to the preferred embodiment of the present invention, other and further embodiments of the invention may be devised without departing from the basic scope thereof, and the scope thereof is determined by the claims that follow.
Claims (9)
1. A preparation method of a novel biological enzyme algaecide for river water body treatment is characterized by comprising the following steps:
(1) adding enzyme into a proper amount of water to prepare an enzyme solution, and adding the prepared enzyme solution into a sodium alginate solution to obtain a mixed solution A;
(2) adding the sterilized modified zeolite into the microbial bacterium mixed suspension D, oscillating for adsorption, and standing for a period of time to obtain a bacterium liquid mixture B;
(3) adding the bacterial liquid mixture B obtained in the step (2) into the mixed liquid A obtained in the step (1), and uniformly mixing to obtain a mixed liquid C;
(4) and (3) adding a calcium chloride solution into the mixed solution C to perform a crosslinking reaction, and washing with sterile water after the crosslinking reaction is finished to obtain the novel biological enzyme algaecide.
2. The preparation method of the novel biological enzyme algaecide for treating the river water body according to claim 1, wherein the raw materials of the novel biological enzyme algaecide comprise the following components in parts by weight:
10-15 parts of modified zeolite;
15-25 parts of mixed bacteria;
30-40 parts of cellulase;
10-20 parts of pectinase;
the mixed bacteria include at least 4 of slime bacteria, Bacillus subtilis, yeast, phosphorus-accumulating bacteria, and ammonia nitrogen bacteria.
3. The preparation method of the novel biological enzyme algaecide for river water body treatment according to claim 1, wherein the preparation method comprises the following steps: the enzyme solution in the step (1) is formed by mixing at least two biological enzymes of pectinase and cellulase, and the mass concentration of the sodium alginate solution is 3-4%.
4. The preparation method of the novel biological enzyme algaecide for river water body treatment according to claim 1, wherein the preparation method comprises the following steps: the modified zeolite in the step (2) is 200-mesh zeolite modified by acid; the sterilization temperature is 121 ℃, and the sterilization time is 15 min; the oscillation speed of the oscillation adsorption is 150r/min, and the oscillation time is 30 min; the bacterial liquid mixture B mainly comprises a viscous bacterial group, a bacillus subtilis group, a yeast group, a phosphorus-accumulating group and an ammonia nitrogen group; the standing time is 24 hours, and the standing temperature is 25 ℃.
5. The preparation method of the novel biological enzyme algaecide for river water body treatment according to claim 1, wherein the preparation method comprises the following steps: the mass concentration of the calcium chloride solution in the step (4) is 2-4%, and the crosslinking reaction is to place the mixed solution C in a refrigerator at 4 ℃ for 24 hours.
6. A novel biological enzyme algaecide for river water body treatment is characterized in that: the novel biological enzyme algaecide for treating the river water body is prepared by the method of any one of claims 1 to 5.
7. The use method of the novel biological enzyme algaecide for the river water body treatment according to any one of claims 1 to 6 is characterized by comprising the following steps: the novel biological enzyme algaecide is put into a medicine bag and then put into a fixed position in a polluted river, and the putting amount of the novel biological enzyme algaecide is 500-1000 g/m 2 。
8. The method for recovering the novel bio-enzyme algaecide for river water body treatment according to claim 7, wherein the recovery method comprises the following steps: the novel biological enzyme algaecide for treating the river water body after sewage treatment is washed by sterile water for 3 times or more, and can be repeatedly used.
9. The method for recovering the novel bio-enzyme algaecide for river water body treatment according to claim 8, wherein the method comprises the following steps: the repeatedly used novel biological enzyme algaecide has the advantages that the algae removal rate is over 70 percent, the ammonia nitrogen and total phosphorus removal rate is over 60 percent, and the repeated use stability is good.
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