CN111892173A - Preparation method of enzyme composite liquid and method for purifying sewer sewage and air by using enzyme composite liquid - Google Patents
Preparation method of enzyme composite liquid and method for purifying sewer sewage and air by using enzyme composite liquid Download PDFInfo
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Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
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- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
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- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
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- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Water Supply & Treatment (AREA)
- Organic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Treatment Of Sludge (AREA)
Abstract
The invention relates to the technical field of sewage treatment, in particular to a preparation method of a ferment composite liquid and a method for purifying sewage and air by using the ferment composite liquid. The method comprises the following steps: the first fermentation raw material containing brown sugar is subjected to first fermentation by utilizing contained sugar and leftovers of melon and fruit vegetables or melon and fruit vegetables to obtain first fermentation liquid. Adding fruit and vegetable or fruit and vegetable leftovers into the first fermentation liquid, and fermenting to obtain a second fermentation liquid. And filtering, purifying, adding bacillus and culturing the second fermentation liquid to obtain the ferment complex liquid, wherein the total colony population contained in the ferment complex liquid is 20X 200-20X 300CFU/ml, the effective viable bacteria content is more than 60-80%, and the digestive decomposition enzyme content is more than 10-20%. The ferment composite liquid is directly sprayed into a sewer, and the breeding of harmful flora is inhibited under the action of effective viable bacteria and decomposition enzyme in beneficial flora in the composite liquid.
Description
Technical Field
The invention relates to the technical field of sewage treatment, in particular to a preparation method of a ferment composite liquid and a method for purifying sewage and air by using the ferment composite liquid.
Background
The information disclosed in this background of the invention is only for enhancement of understanding of the general background of the invention and is not necessarily to be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person skilled in the art.
Along with the acceleration of the urbanization process, the sewers in urban areas are more and longer, more and more toxic, harmful and smelly gases are discharged from the sewage outlet of the sewer every day, and the environmental pollution is more and more serious. Along with the increasing population of cities, the pollution to urban environment is more and more serious, infectious disease bacteria in air in urban spaces are more and more, the traditional treatment method of urban sewers is that sewage enters a treatment plant through a sewer for treatment and then is discharged into rivers, lakes and seas, and rainwater is directly discharged into the rivers, lakes and seas through a rainwater sewer. The traditional method for purifying air in urban space mostly adopts a method of sprinkling water for purification or spraying by using an urban road sprinkler for air purification.
However, the conventional treatment method for urban sewers and air purification has many problems, for example, although the air is purified just after being sprayed by a sprinkler or a spray, harmful bacteria in the air cannot be controlled, harmful bacteria are re-introduced into the air along with steam as the sprayed water evaporates, some bacteria float into the air again along with dust, thus further harming human health, and large-particle dust carries harmful bacteria to enter a sewage treatment plant along with rainwater through the sewers. The sewage treated in the sewage treatment plant is generally subjected to sedimentation, disinfection and decomposition, a large amount of chemicals are adopted in order to reach the rapid discharge standard, such as flocculating agents and the like, although toxic and harmful substances are controlled and reach the discharge standard of the sewage, a large part of beneficial flora is also destroyed, so that the activity of discharged water is greatly reduced, and the secondary pollution of the water is easily caused. The rainwater in the rainwater sewer is directly discharged into rivers, lakes and seas through the rainwater sewer, and organic matters, toxic and harmful substances which are not decomposed in the rainwater sewer are discharged into the rivers, the lakes and the seas along with the rainwater, so that the ecology of the rivers, the lakes and the seas is necessarily polluted. Even if the water discharged from the sewage treatment plant does not cause secondary pollution to rivers, lakes and seas, and the water discharged from the rainwater sewers does not cause pollution to the ecology of rivers, lakes and seas, a large amount of organic matters and toxic and harmful substances in the sewage sewers or the rainwater sewers still remain, and particularly in seasons with little rainwater. Residual organic matters and toxic and harmful substances in the sewer are slowly decomposed in the sewer, harmful flora can not be controlled due to the special environment in the sewer, and beneficial flora is inhibited from propagating and growing, so that only the harmful flora decomposes the organic matters and the toxic and harmful substances in the sewer, and a large amount of toxic, harmful and malodorous gas is released to influence the health of citizens and pollute the environment.
Disclosure of Invention
In order to solve the problems, the invention provides a preparation method of a ferment composite liquid and a method for purifying sewage and air by using the ferment composite liquid. The ferment composite liquid is directly sprayed into a sewer, so that the breeding of harmful flora is inhibited under the action of effective viable bacteria and decomposition enzyme in beneficial flora in the composite liquid, and organic matters and toxic and harmful substances in the sewer are decomposed in the sewer, so that toxic, harmful and malodorous gases are not discharged from a sewer opening of the sewer; and directly spraying the ferment composite liquid into the air when purifying the air.
In order to achieve the above object, the technical solution of the present invention is as follows.
In a first aspect of the present invention, a method for preparing a complex enzyme solution is disclosed, which comprises the following steps:
a first fermentation stage: performing first fermentation on a first fermentation raw material containing brown sugar by using the contained sugar and the leftovers of melon and fruit vegetables or melon and fruit vegetables to obtain a first fermentation solution;
a second fermentation stage: adding fruits and vegetables or fruit and vegetable leftovers into the first fermentation liquid, and fermenting to obtain a second fermentation liquid;
enzyme complex liquid: and filtering, purifying, adding bacillus and culturing the second fermentation liquid to obtain the ferment complex liquid, wherein the total colony population contained in the ferment complex liquid is 20X 200-20X 300CFU/ml, the effective viable bacteria content is more than 60-80%, and the digestive decomposition enzyme content is more than 10-20%.
In a second aspect of the present invention, a method for purifying sewage by using the ferment composite liquid prepared by the above method is disclosed: and directly adding the ferment composite liquid into sewage in a sewer.
In a third aspect of the present invention, a method for purifying air by using the enzyme complex liquid prepared by the above method is disclosed: adding the ferment composite liquid into spray, and then spraying the spray into air; the concentration of the ferment complex liquid is 10-30%, the diluent is clear water, and the preferable concentration of the ferment complex liquid is 30%. By adopting the steps, the breeding of harmful bacteria in the air can be effectively inhibited, and the enzyme compound liquid can be saved to the greatest extent.
Compared with the prior art, the invention has the following beneficial effects:
(1) when the invention is used for purifying the air in urban space, various microorganisms, effective live bacteria and decomposition enzymes in the enzyme complex liquid are sprayed out along with spraying and sprinkling water, and simultaneously are combined with dust in the air and harmful bacteria in the air to form large-particle water drops which fall to the ground along with water and enter a sewer for decomposition and digestion, the harmful bacteria which cannot enter the sewer are inhibited under the action of various enzymes in the enzyme complex liquid, so that the harmful bacteria cannot obtain effective energy, lose activity and lose disease propagation capacity, and then are slowly decomposed under the action of the decomposition enzymes.
(2) The living bacteria, microorganisms and various decomposition enzymes in the ferment complex liquid form a stable structure in the processes of fission, propagation and growth, the required energy has a wide variety of biological floras of the microorganisms, and the biological floras of the microorganisms cooperate with organic matters and toxic and harmful substances in a sewer, so that the growth and the propagation of the harmful microorganisms are inhibited, the beneficial floras are symbiotic and synergestic in the sewer, and the effect of decomposing the organic matters and the toxic and harmful substances in the sewer is achieved. The sewage outlet of the sewer can not discharge poisonous, harmful and malodorous gas any more, and the water discharged by the sewer can not be used for removing water containing pollutants from the rivers, lakes and seabelts any more, thereby achieving the purpose of purifying the environment.
Detailed Description
It is to be understood that the following detailed description is exemplary and is intended to provide further explanation of the invention as claimed. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the invention. As used herein, the singular is intended to include the plural unless the context clearly dictates otherwise. Furthermore, it will be further understood that the terms "comprises" and/or "comprising," when used in this specification, specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof.
As described above, the organic substances and toxic and harmful substances remaining in the sewer are slowly decomposed in the sewer, the harmful flora is not controlled due to the special environment in the sewer, and the beneficial flora is inhibited from propagating and growing, so that only the harmful flora decomposes the organic substances and the toxic and harmful substances in the sewer, and a large amount of toxic, harmful and malodorous gases are released to influence the health of citizens and pollute the environment. Therefore, the invention provides a preparation method of the ferment composite liquid and a method for purifying sewage and air by using the ferment composite liquid;
a first fermentation stage: performing first fermentation on a first fermentation raw material containing brown sugar by using the contained sugar and the leftovers of melon and fruit vegetables or melon and fruit vegetables to obtain a first fermentation solution;
a second fermentation stage: adding fruits and vegetables or fruit and vegetable leftovers into the first fermentation liquid, and fermenting to obtain a second fermentation liquid;
enzyme complex liquid: and filtering, purifying, adding bacillus and culturing the second fermentation liquid to obtain the ferment complex liquid, wherein the total colony population contained in the ferment complex liquid is 20X 200-20X 300CFU/ml, the effective viable bacteria content is more than 60-80%, and the digestive decomposition enzyme content is more than 10-20%.
Further, the raw materials of the first fermentation stage comprise 5-10 parts by weight of brown sugar and 5-10 parts by weight of melons and fruits vegetables or leftovers of melons and fruits vegetables.
Or the raw materials of the first fermentation stage comprise 5-10 parts by weight of brown sugar and 5-10 parts by weight of leaf vegetables or leftovers of the leaf vegetables.
Furthermore, the raw materials of the second fermentation stage comprise 5-10 parts by weight of leftovers of melon and fruit vegetables or melon and fruit vegetables, 5-10 parts by weight of leftovers of leaf vegetables or leaf vegetables and 5-10 parts by weight of fruits.
Or the raw materials of the second fermentation stage comprise 10-20 parts by weight of melon and fruit vegetables or leftovers of melon and fruit vegetables and 10-20 parts by weight of leaf vegetables or leftovers of leaf vegetables.
Or the raw materials of the first fermentation stage comprise 5-10 parts by weight of fruits, and the second fermentation raw materials comprise 20-30 parts by weight of fruits.
In the invention, the fermentation raw materials are rich and various according to local conditions, can change at any time and are not limited by time, environment and seasonal changes.
Further, the brown sugar includes a raw brown sugar for daily use and a brown sugar for industrial use, and the brown sugar for industrial use is preferred in the invention. The melon and fruit vegetables comprise tomatoes, cucumbers, radishes, cauliflowers, eggplants, pumpkin, white gourds and the like, and the tomatoes, the cucumbers, the radishes, the pumpkin and the eggplants are preferably selected in the invention.
Further, the leafy vegetable includes: cabbage, radish leaf, cauliflower leaf, cabbage leaf, broccoli leaf and the like or leftovers thereof. The invention preferably selects Chinese cabbage, radish leaf, cauliflower leaf, broccoli leaf, cabbage leaf or leftovers thereof.
Further, the fruit comprises: apple, banana, dragon fruit, peach, grape, pear and orange. The invention is preferably selected from apple, pear and orange. The fruit can also be replaced by pumpkin seed leftovers.
Further, according to the method of the present invention, the amount of brown sugar added in the first fermentation stage is 5 to 10 parts by weight, preferably 9 to 10 parts by weight, for example 10 parts by weight, of the present invention, and 5 to 10 parts by weight of the leftovers of the melon and fruit vegetables or the melon and fruit vegetables, preferably 8 to 10 parts by weight, for example 10 parts by weight, of the present invention. In the second fermentation stage, 5-10 parts by weight of melons, fruits and vegetables or leftovers of melons, fruits and vegetables are added into the first fermentation solution, and 10 parts by weight is preferably selected; adding 5-10 parts by weight of leaf vegetables or leftovers of the leaf vegetables, preferably 8 parts by weight; 5-10 parts by weight of fruits are added, and 10 parts by weight is preferred in the invention.
Further, according to the method, the first fermentation time is 2 days, the fermentation temperature is 10-40 ℃, the temperature is preferably 20-30 ℃, and beneficial bacteria are generated in the fermentation by adopting the steps.
Further, according to the method, the second fermentation time is 80-90 days, the preferred fermentation time is 90 days, the fermentation temperature is 20-40 ℃ (preferably 25-30 ℃) in the first 20-30 days, the fermentation temperature is 20-40 ℃ (preferably 20-30 ℃) in the last 60 days, and the steps are adopted to be beneficial to generation of beneficial bacterium groups and decomposition enzyme in the fermentation process.
Further, according to the method, within 2 days of the first fermentation stage, stirring is carried out for 5-10 minutes, preferably 7 minutes every day, and standing is carried out for closed fermentation after stirring. And (3) opening the cover and stirring for 5-20 minutes every day in the first 20-30 days of the second fermentation for 90 days, preferably 10 minutes, standing for 60 days, and sealing for fermentation, wherein the steps are adopted to ensure that the fermentation is more uniform and more beneficial flora and decomposition enzyme are generated.
Further, according to the method, the culture time after filtration, purification and addition of the bacillus is 4-7 days, preferably 5 days, the culture temperature is 10-40 ℃, and preferably 20-30 ℃. By adopting the steps, the production of beneficial live bacteria and decomposition enzyme in the beneficial bacteria in the ferment composite liquid can be effectively improved.
Further, according to the method, a filter with 100-300 meshes, preferably a filter with 150 meshes is adopted for filtering. And adding 10-20 times of natural fresh water by weight into the filtered second fermentation broth, and preferably selecting 10 times of well water. The invention preferably adds 5-10 parts by weight of 100-1000 hundred million/g bacillus, preferably 5 parts by weight of 200 hundred million/g bacillus; preferably, the bacillus is added and stirred for 5 to 10 minutes, preferably 10 minutes. And finally, standing and sealing for culture. The steps are adopted to culture more effective viable bacteria of beneficial flora and split more decomposition enzymes.
Further, the solid matter obtained by filtering can be added into municipal domestic waste, and is fermented to obtain a biological fertilizer meeting the national GB 20287-2006 standard, and the biological fertilizer is used for soil improvement and crop yield increase.
Further, the solid matter obtained by filtering can be added into wheat straws and corn straws for fermentation to obtain the non-anti-immune feed which meets 2016-2020 national bacteria resistance inhibition action plan and 2017-2020 national animal source bacteria resistance inhibition action plan.
Furthermore, the concentration of the enzyme complex liquid is 10-30%, the diluent is clear water, and the optimal concentration is 20% in the invention.
Further, the ferment composite liquid can be directly sprayed into a sewer according to batches, so that the ferment composite liquid is fully combined with organic matters and toxic and harmful substances in the sewer, and the spraying interval time is as follows: 5-10 days in spring, preferably 7 days in summer, 10-30 days in summer, preferably 21 days in the invention, 10-20 days in winter in autumn, preferably 14 days in the invention, and the implementation times are properly reduced in summer. The method is helpful for further promoting the organic matters and the toxic and harmful substances in the sewer to be fully decomposed, thereby achieving the purpose of treating the urban sewer.
The sewage includes industrial sewage, municipal rainwater, etc., but the enzyme composite liquid of the present invention is preferably municipal sewage and municipal rainwater in view of treatment effect.
According to one embodiment of the present invention, the prepared ferment compound liquid is directly sprayed into the air in the space, so that the ferment compound liquid can be in contact with dust or harmful bacteria in the air, and the air can be maximally purified. The spraying time is once every 2-3 days, and the spraying time is once every day when the air pollution is serious.
The invention will now be further illustrated with reference to specific examples.
First embodiment
Adding 8 parts by weight of brown sugar and 8 parts by weight of watermelon peel into 100 parts by weight of natural fresh water during first fermentation, sealing and fermenting for 2 days at the temperature of 30 ℃, stirring for 15 minutes every day to obtain a first fermentation solution, adding 8 parts by weight of watermelon peel, 8 parts by weight of celery leaves and 5-10 parts by weight of apples into the first fermentation solution during second fermentation, fermenting for 90 days, wherein the fermentation temperature of the first 30 days is 30 ℃, the stirring is carried out for 12 minutes every day, the fermentation temperature of the subsequent 60 days is 30 ℃, the standing and closed fermentation are carried out to obtain a second fermentation liquid, the second fermentation liquid is filtered and purified by a 100-300-mesh filter, then 15 parts of natural fresh water is added, then 8 parts by weight of bacillus of 600 hundred million/g is added, the mixture is cultured for 7 days in a closed way, the culture temperature is 30 ℃, and the mixture is stirred evenly and then is kept stand for closed culture, thus obtaining the ferment complex liquid which is suitable for treating urban sewers and purifying air in urban spaces.
The bacterial colony number of the ferment complex liquid is 20 × 257cfu/ml, the content of effective viable bacteria is more than 72%, and the content of digestive decomposition enzyme is more than 18%, so that the treatment of urban sewers and urban space air is carried out.
Taking 100ml of sewage in a city sewer, adding 5 times of natural fresh water, and directly spraying the prepared ferment complex liquid into the sewage after dilution to ensure that the sewage and the sewage are in full contact, wherein the using amount is 0.35g/100ml, and the treatment time is 7 days.
Second embodiment
Adding 5 parts by weight of brown sugar and 5 parts by weight of watermelon peel into 100 parts by weight of natural fresh water during first fermentation, sealing and fermenting for 2 days at the temperature of 10 ℃, stirring for 10 minutes every day to obtain a first fermentation solution, adding 5 parts by weight of Hami melon peel, 5-10 parts by weight of celery leaves and 5-10 parts by weight of peaches into the first fermentation solution during second fermentation, fermenting for 90 days, wherein the fermentation temperature of the first 30 days is 20 ℃, the stirring is carried out for 20 minutes every day, the fermentation temperature of the subsequent 60 days is 20 ℃, the standing and closed fermentation are carried out to obtain a second fermentation liquid, the second fermentation liquid is filtered and purified by a 100-300 mesh filter, then 10 natural fresh water is added, then adding 5 parts by weight of 200 hundred million/g bacillus, carrying out closed culture for 5 days at the culture temperature of 45 ℃, uniformly stirring, standing, and carrying out closed culture to obtain the ferment complex liquid which is suitable for treating urban sewers and purifying air in urban spaces.
The bacterial colony number of the ferment complex liquid is 20 x 219cfu/ml, the content of effective viable bacteria is more than 60%, and the content of digestive decomposition enzyme is more than 10%, so that the treatment of urban sewers and urban space air is carried out.
Taking 100ml of sewage in a city sewer, adding 5 times of natural fresh water, and directly spraying the prepared ferment complex liquid into the diluted sewage to ensure that the ferment complex liquid and the diluted sewage are in full contact, wherein the using amount is 0.35g/100ml, and the treatment time is 7 days.
Third embodiment
Adding 10 parts by weight of brown sugar and 10 parts by weight of melon peel into 100 parts by weight of natural fresh water during first fermentation, sealing and fermenting for 2 days at the temperature of 40 ℃, stirring for 20 minutes every day to obtain a first fermentation solution, adding 10 parts by weight of watermelon peel, 5-10 parts by weight of celery leaves and 10 parts by weight of pears into the first fermentation solution during second fermentation, fermenting for 90 days, wherein the fermentation temperature of the first 30 days is 40 ℃, the stirring is carried out for 20 minutes every day, the fermentation temperature of the subsequent 60 days is 40 ℃, the standing and closed fermentation are carried out to obtain a second fermentation liquid, the second fermentation liquid is filtered and purified by a 100-300 mesh filter, then 20 natural fresh water is added, then adding 10 parts by weight of bacillus 1000 hundred million/g, carrying out closed culture for 7 days at the culture temperature of 45 ℃, uniformly stirring, standing, and carrying out closed culture to obtain the ferment complex liquid which is suitable for treating urban sewers and purifying air in urban spaces.
The bacterial colony number of the ferment complex liquid is 20 x 297cfu/ml, the content of effective viable bacteria is more than 80%, and the content of digestive decomposition enzyme is more than 20%, so that the treatment of urban sewers and urban space air is carried out.
Taking 100ml of sewage in a city sewer, adding 5 times of natural fresh water, and directly spraying the prepared ferment complex liquid into the diluted sewage to ensure that the ferment complex liquid and the diluted sewage are in full contact, wherein the using amount is 0.35g/100ml, and the treatment time is 7 days.
Fourth embodiment
Adding 5-10 parts by weight of brown sugar and 5-10 parts by weight of watermelon peel into 100 parts by weight of natural fresh water during first fermentation, performing closed fermentation for 2 days at the temperature of 30 ℃ for 15 minutes every day to obtain a first fermentation solution, adding 8 parts by weight of watermelon peel, 8 parts by weight of celery leaves and 5-10 parts by weight of apples into the first fermentation solution during second fermentation, performing fermentation for 90 days, wherein the fermentation temperature of the first 30 days is 30 ℃, stirring for 12 minutes every day, the fermentation temperature of the subsequent 60 days is 30 ℃, standing for closed fermentation to obtain a second fermentation solution, filtering and purifying the second fermentation solution by using a 100-300-mesh filter, adding 15 parts by weight of natural fresh water, adding 8 parts by weight of bacillus 600 hundred million/gram, performing closed culture for 7 days, performing uniform stirring, and performing standing closed culture to obtain a treated urban sewer, the ferment composite liquid can purify the air in the urban space.
The bacterial colony number of the ferment complex liquid is 20 × 257cfu/ml, the content of effective viable bacteria is more than 72%, and the content of digestive decomposition enzyme is more than 18%, so that the treatment of urban sewers and urban space air is carried out.
When purifying air in urban space, 20m3The amount of the prepared enzyme composite liquid is directly sprayed into the air when the air of the urban space is purified, and the amount of the prepared enzyme composite liquid is 4kg/1000m3The treatment time was 1 day.
Fifth embodiment
Adding 8 parts by weight of brown sugar and 8 parts by weight of watermelon peel into 100 parts by weight of natural fresh water during first fermentation, sealing and fermenting for 2 days at the temperature of 10 ℃, stirring for 10 minutes every day to obtain a first fermentation solution, adding 5 parts by weight of Hami melon peel, 5-10 parts by weight of celery leaves and 5-10 parts by weight of peaches into the first fermentation solution during second fermentation, fermenting for 90 days, wherein the fermentation temperature of the first 30 days is 20 ℃, the stirring is carried out for 20 minutes every day, the fermentation temperature of the subsequent 60 days is 20 ℃, the standing and closed fermentation are carried out to obtain a second fermentation liquid, the second fermentation liquid is filtered and purified by a 100-300 mesh filter, then 10 natural fresh water is added, then adding 5 parts by weight of 200 hundred million/g bacillus, carrying out closed culture for 5 days at the culture temperature of 45 ℃, uniformly stirring, standing, and carrying out closed culture to obtain the ferment complex liquid which is suitable for treating urban sewers and purifying air in urban spaces.
The bacterial colony number of the ferment complex liquid is 20 x 219cfu/ml, the content of effective viable bacteria is more than 60%, and the content of digestive decomposition enzyme is more than 10%, so that the treatment of urban sewers and urban space air is carried out.
When purifying air in urban space, 20m3The amount of the prepared enzyme composite liquid is directly sprayed into the air when the air of the urban space is purified, and the amount of the prepared enzyme composite liquid is 4kg/1000m3The treatment time was 1 day.
Sixth embodiment
Adding 10 parts by weight of brown sugar and 10 parts by weight of melon peel into 100 parts by weight of natural fresh water during first fermentation, sealing and fermenting for 2 days at the temperature of 40 ℃, stirring for 20 minutes every day to obtain a first fermentation solution, adding 10 parts by weight of watermelon peel, 5-10 parts by weight of celery leaves and 10 parts by weight of pears into the first fermentation solution during second fermentation, fermenting for 90 days, wherein the fermentation temperature of the first 30 days is 40 ℃, the stirring is carried out for 20 minutes every day, the fermentation temperature of the subsequent 60 days is 40 ℃, the standing and closed fermentation are carried out to obtain a second fermentation liquid, the second fermentation liquid is filtered and purified by a 100-300 mesh filter, then 20 natural fresh water is added, then adding 10 parts by weight of bacillus 1000 hundred million/g, carrying out closed culture for 7 days at the culture temperature of 45 ℃, uniformly stirring, standing, and carrying out closed culture to obtain the ferment complex liquid which is suitable for treating urban sewers and purifying air in urban spaces.
The bacterial colony number of the ferment complex liquid is 20 x 297cfu/ml, the content of effective viable bacteria is more than 80%, and the content of digestive decomposition enzyme is more than 20%, so that the treatment of urban sewers and urban space air is carried out.
When purifying air in urban space, 20m3The amount of the prepared enzyme composite liquid is directly sprayed into the air when the air of the urban space is purified, and the amount of the prepared enzyme composite liquid is 4kg/1000m3The treatment time was 1 day.
TABLE 1
| Detecting the index | COD(mg/L) | BOD(mg/L) |
| Sewage of urban sewer | 480 | 296 |
| Example 1 | 360 | 182 |
| Example 2 | 210 | 105 |
| Example 3 | 120 | 89 |
TABLE 2
Finally, it should be understood that any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention. Although the foregoing description describes embodiments of the present invention, it is not intended to limit the scope of the present invention, and those skilled in the art will appreciate that various modifications and variations can be made without departing from the spirit and scope of the present invention.
Claims (10)
1. The preparation method of the ferment complex liquid is characterized by comprising the following steps of:
a first fermentation stage: performing first fermentation on a first fermentation raw material containing brown sugar by using the contained sugar and the leftovers of melon and fruit vegetables or melon and fruit vegetables to obtain a first fermentation solution;
a second fermentation stage: adding fruits and vegetables or fruit and vegetable leftovers into the first fermentation liquid, and fermenting to obtain a second fermentation liquid;
enzyme complex liquid: and filtering, purifying, adding bacillus and culturing the second fermentation liquid to obtain the ferment complex liquid, wherein the total colony population contained in the ferment complex liquid is 20X 200-20X 300CFU/ml, the effective viable bacteria content is more than 60-80%, and the digestive decomposition enzyme content is more than 10-20%.
2. The preparation method of the ferment complex liquid according to claim 1, wherein the raw materials of the first fermentation stage comprise 5 to 10 parts by weight of brown sugar, 5 to 10 parts by weight of fruits and vegetables or leftovers of fruits and vegetables;
or the raw materials of the first fermentation stage comprise 5-10 parts by weight of brown sugar and 5-10 parts by weight of leaf vegetables or leftovers of the leaf vegetables.
3. The preparation method of the ferment complex liquid according to claim 1, wherein the raw materials of the second fermentation stage comprise 5-10 parts by weight of fruits and vegetables or leftovers of fruits and vegetables, 5-10 parts by weight of leaves and vegetables or leftovers, and 5-10 parts by weight of fruits;
or the raw materials of the second fermentation stage comprise 10-20 parts by weight of melon and fruit vegetables or leftovers of melon and fruit vegetables and 10-20 parts by weight of leaf vegetables or leftovers of leaf vegetables;
or the raw materials of the first fermentation stage comprise 5-10 parts by weight of fruits, and the second fermentation raw materials comprise 20-30 parts by weight of fruits.
4. The method for preparing the ferment complex liquid according to claim 1, wherein the brown sugar comprises a raw brown sugar, an industrial brown sugar, preferably an industrial brown sugar; the melon and fruit vegetables comprise tomatoes, cucumbers, radishes, cauliflowers, eggplants, pumpkin, pumpkins and white gourds, and preferably tomatoes, cucumbers, radishes, pumpkin and eggplants;
alternatively, the leafy vegetables include: chinese cabbage, radish leaf, cauliflower leaf, cabbage leaf, broccoli leaf or their leftovers; preferably Chinese cabbage, radish leaf, cauliflower leaf, broccoli leaf, cabbage leaf or leftovers thereof;
alternatively, the fruit comprises: apple, banana, dragon fruit, peach, grape, pear, orange; preferably apple, pear, orange; or the fruit is replaced by pumpkin seed leftovers and/or black melon seed leftovers.
5. The method for preparing the ferment complex liquid according to claim 1, wherein the brown sugar is added in an amount of 5 to 10 parts by weight, preferably 9 to 10 parts by weight, in the first fermentation stage; 5-10 parts by weight of melon and fruit vegetables or leftovers of melon and fruit vegetables, preferably 8-10 parts by weight;
in the second fermentation stage, 5-10 parts by weight of melons and fruits or leftovers of melons and fruits vegetables are added into the first fermentation liquid, and 10 parts by weight is preferred; adding 5-10 parts by weight of leaf vegetables or leftovers of the leaf vegetables, preferably 8 parts by weight; adding 5-10 parts by weight of fruits, preferably 10 parts by weight;
or the first fermentation time is 2 days, the fermentation temperature is 10-40 ℃, and the optimal fermentation temperature is 20-30 ℃;
or the second fermentation time is 80-90 days, preferably 90 days, and the fermentation temperature is 20-40 ℃, preferably 25-30 ℃ in the first 20-30 days; the fermentation temperature of the last 60 days is 20-40 ℃, and the optimal fermentation temperature is 20-30 ℃;
or stirring for 5-10 minutes, preferably 7 minutes every day within 2 days of the first fermentation stage, standing for closed fermentation after stirring; and (3) opening the cover and stirring for 5-20 minutes, preferably 10 minutes, every day before 20-30 days of 90 days of the second fermentation, and then standing for 60 days for closed fermentation.
6. The method for preparing the enzyme complex liquid according to claim 1, wherein the culturing time after filtration, purification and addition of the bacillus is 4 to 7 days, preferably 5 days, and the culturing temperature is 10 to 40 ℃, preferably 20 to 30 ℃;
preferably, a filter with 100-300 meshes is adopted for filtering, and a filter with 150 meshes is preferably adopted for filtering;
preferably, 10-20 times of natural fresh water by weight, preferably 10 times of well water, is added into the filtered second fermentation broth;
preferably, 5 to 10 parts by weight of 100 to 1000 parts by weight of Bacillus, preferably 5 parts by weight of 200 parts by weight of Bacillus; preferably, the bacillus is added and stirred for 5-10 minutes, and then the mixture is kept stand and sealed for culture;
preferably, the solid matter obtained by filtering can be added into municipal domestic waste, and is fermented to obtain a biological fertilizer meeting the national GB 20287-2006 standard;
preferably, the solid matter obtained by filtering can be added into wheat straws and corn straws for fermentation to obtain the non-anti-immune feed meeting the national bacterial resistance inhibition action plan 2016-2020 and the national animal source bacterial resistance inhibition action plan 2017-2020.
7. The method for purifying sewage water by using the ferment complex liquid prepared by the method of any one of claims 1 to 6, wherein the ferment complex liquid is directly added into sewage water in a sewer;
preferably, the concentration of the enzyme complex liquid is 10-30%, and the diluent is clear water, more preferably 20%;
or the ferment composite liquid is directly sprayed into a sewer according to batches, so that the ferment composite liquid is fully combined with organic matters and toxic and harmful substances in the sewer, and the spraying interval time is as follows: 5-10 days in spring, 10-30 days in summer and 10-20 days in autumn and winter; the preferred interval between injections is: spring 7 days, summer 21 days, autumn winter 14 days.
8. Method for purifying sewage water according to claim 7, wherein said sewage water comprises one or more of industrial sewage water, municipal rainwater, preferably municipal sewage water, municipal rainwater.
9. A method for purifying air by using the ferment complex liquid prepared by the method of any one of claims 1 to 6, comprising the following steps: adding the ferment composite liquid into spray, and then spraying the spray into air; the concentration of the ferment composite liquid is 10-30%, and the diluent is clear water, preferably 30%.
10. The method for purifying air according to claim 9, wherein the spraying is performed for 2 to 3 days.
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