CN112746040A - Organic garbage biodegradation strain - Google Patents
Organic garbage biodegradation strain Download PDFInfo
- Publication number
- CN112746040A CN112746040A CN202110013642.5A CN202110013642A CN112746040A CN 112746040 A CN112746040 A CN 112746040A CN 202110013642 A CN202110013642 A CN 202110013642A CN 112746040 A CN112746040 A CN 112746040A
- Authority
- CN
- China
- Prior art keywords
- strains
- parts
- organic
- garbage
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000010813 municipal solid waste Substances 0.000 title claims abstract description 111
- 238000006065 biodegradation reaction Methods 0.000 title claims abstract description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 53
- 238000006731 degradation reaction Methods 0.000 claims abstract description 41
- 230000015556 catabolic process Effects 0.000 claims abstract description 36
- 235000015097 nutrients Nutrition 0.000 claims abstract description 29
- 238000000034 method Methods 0.000 claims abstract description 18
- 241000894006 Bacteria Species 0.000 claims abstract description 7
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 6
- 241000193399 Bacillus smithii Species 0.000 claims abstract description 6
- 241000193385 Geobacillus stearothermophilus Species 0.000 claims abstract description 6
- 230000000593 degrading effect Effects 0.000 claims abstract description 6
- 239000000463 material Substances 0.000 claims abstract description 6
- 239000000126 substance Substances 0.000 claims description 29
- 241000252185 Cobitidae Species 0.000 claims description 25
- 241000252496 Siluriformes Species 0.000 claims description 25
- 230000001580 bacterial effect Effects 0.000 claims description 23
- 239000010802 sludge Substances 0.000 claims description 22
- 238000003756 stirring Methods 0.000 claims description 17
- 238000005273 aeration Methods 0.000 claims description 15
- 239000008223 sterile water Substances 0.000 claims description 15
- 239000010815 organic waste Substances 0.000 claims description 14
- 241000894007 species Species 0.000 claims description 12
- 238000012258 culturing Methods 0.000 claims description 10
- 239000002689 soil Substances 0.000 claims description 10
- 239000004568 cement Substances 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 7
- 241000186361 Actinobacteria <class> Species 0.000 claims description 5
- 229920001817 Agar Polymers 0.000 claims description 5
- 241000272525 Anas platyrhynchos Species 0.000 claims description 5
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 5
- 241000228143 Penicillium Species 0.000 claims description 5
- 241000589517 Pseudomonas aeruginosa Species 0.000 claims description 5
- 239000008272 agar Substances 0.000 claims description 5
- 239000008280 blood Substances 0.000 claims description 5
- 210000004369 blood Anatomy 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 238000010907 mechanical stirring Methods 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 239000010902 straw Substances 0.000 claims description 5
- 239000000725 suspension Substances 0.000 claims description 5
- 108010059892 Cellulase Proteins 0.000 claims description 4
- 108091005804 Peptidases Proteins 0.000 claims description 4
- 239000004365 Protease Substances 0.000 claims description 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 4
- 229920002472 Starch Polymers 0.000 claims description 4
- 229940106157 cellulase Drugs 0.000 claims description 4
- 239000001913 cellulose Substances 0.000 claims description 4
- 229920002678 cellulose Polymers 0.000 claims description 4
- 238000007599 discharging Methods 0.000 claims description 4
- 102000004169 proteins and genes Human genes 0.000 claims description 4
- 108090000623 proteins and genes Proteins 0.000 claims description 4
- 239000008107 starch Substances 0.000 claims description 4
- 235000019698 starch Nutrition 0.000 claims description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 2
- 229910052760 oxygen Inorganic materials 0.000 claims description 2
- 239000001301 oxygen Substances 0.000 claims description 2
- 230000008569 process Effects 0.000 abstract description 8
- 238000000354 decomposition reaction Methods 0.000 description 10
- 239000004519 grease Substances 0.000 description 10
- 244000005700 microbiome Species 0.000 description 10
- 238000000855 fermentation Methods 0.000 description 7
- 230000004151 fermentation Effects 0.000 description 7
- 239000002699 waste material Substances 0.000 description 6
- 238000006213 oxygenation reaction Methods 0.000 description 5
- 230000002195 synergetic effect Effects 0.000 description 5
- 230000009471 action Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000010806 kitchen waste Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses an organic garbage biodegradation strain, which comprises at least two or more of bacillus smithii, bacillus stearothermophilus and bacillus licheniformis in parts by weight of 20-30 parts, and also comprises the following materials in parts by weight: 10-15 parts of rapid oil-fat reducing strains, 20-30 parts of classified degrading strains, 6-8 parts of extracted essence, 15-20 parts of fouling bacteria, 100 parts of water and 300 parts of basic layer nutrient solution and 25-35 parts of basic layer nutrient solution. The invention relates to the technical field of garbage degradation treatment. The organic garbage biodegradable strain solves the problems that the existing bin body or the organic garbage in the bin body is easy to generate odor and peculiar smell in the processing process, and meanwhile, the garbage after degradation treatment is inconvenient to recycle and has low degradation speed and low degradation rate.
Description
Technical Field
The invention relates to the technical field of garbage degradation treatment, in particular to an organic garbage biodegradation strain.
Background
A fermenter refers to an apparatus used industrially for carrying out microbial fermentation. The main body is generally a main cylinder made of stainless steel plate, and the volume of the main cylinder is 1m3 to hundreds m 3. The tank body or the bin body similar to a fermentation tank is often needed to be used for production and processing in the daily production and processing process, but the used bin body or the garbage in the tank body is difficult to clean, and in the prior art, peculiar smell or odor is easy to generate in the process of treating the organic garbage in the tank body or the bin body, so that the environment around a garbage treatment place is seriously polluted, and meanwhile, after the organic garbage is degraded and treated, the treated garbage is inconvenient to recycle, so that the resource waste is caused, and the problems of low degradation speed and low degradation rate exist in the treatment process.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides an organic garbage biodegradation strain, which solves the problems that the existing organic garbage treatment process is easy to generate odor and peculiar smell, and the garbage after the degradation treatment is inconvenient to recycle, and has low degradation speed and low degradation rate.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: an organic garbage biodegradation strain, which comprises at least two or more of bacillus smithii, bacillus stearothermophilus and bacillus licheniformis in 20-30 parts by weight, and also comprises the following materials in parts by weight: 10-15 parts of rapid oil-fat reducing strains, 20-30 parts of classified degrading strains, 6-8 parts of extracted essence, 15-20 parts of fouling bacteria, 100 parts of water and 300 parts of basic layer nutrient solution and 25-35 parts of basic layer nutrient solution.
Preferably, the water is sterile water cooled to 40 ℃ by 100 ℃ plain water, and the extracted essence is water-soluble essence. Through adopting the sterile water that is cooled to 40 degrees by 100 degrees plain boiled water, can disinfect to sterile water, effectively avoid the influence of water to follow-up bacterial cultivation, through adding water-soluble essence in the bacterial crowd, can effectively reduce the stink and the peculiar smell that produce in rubbish decomposition or the fermentation in using whole bacterial to carry out the degradation process to organic rubbish.
Preferably, the classified degradation strains specifically comprise starch strains, cellulose strains, protein strains, organic substance strains and mixed strains in equal mass ratio, and the bacteria content of the starch strains, the cellulose strains, the protein strains, the organic substance strains and the mixed strains is more than 95%. By adding the classified degradation strains into the whole strains, the whole organic garbage biodegradable strains can degrade various organic matters, and the application range of the whole strains is greatly improved.
Preferably, the specific preparation method of the rapid oil and fat reduction strain comprises the following steps: taking a culture dish with the size of 5cm multiplied by 5cm, adding agar and the fine orange peel slices soaked by the duck blood, and uniformly paving; adding equal mass of fluorescent bacillus, pseudomonas aeruginosa and penicillium into a culture dish, adding the culture dish, keeping the culture temperature at 30-35 ℃, and culturing for 2-4 hours; observing the surface color of the culture dish until the color becomes dark, sucking the bacterial suspension of actinomycetes and mycobacteria into the culture dish through a straw, culturing for 2-3h, and picking out the mould spores and hyphae on the surface to obtain the rapid oil-fat reducing strain. The rapid degradation grease strain is added into the whole strain, so that the whole strain can rapidly degrade grease substances.
Preferably, the fouling bacteria include aerobic and anaerobic flora, cellulase and protease. By adding aerobic and anaerobic flora, cellulase and protease into the whole organic garbage biodegradation strain, the degradation speed and the degradation rate of the whole strain on the organic garbage can be effectively improved.
A method for biodegrading organic waste by treating the organic waste in a silo or tank using an organic waste biodegrading bacterial species of any one of claims 1 to:
step one, adding water and organic garbage biodegradable strains into a bin body or a tank body, standing, and carrying out oxygen aeration treatment from the bottom of the bin body or the tank body at regular time;
step two, performing timed manual stirring or mechanical stirring on the mixture of the bin body or the tank body, and discharging the mixed liquid in the tank body or the bin into a concrete water pool after repeating for a plurality of times;
step three, adding loaches, catfishes and pond sludge into the concrete pond, and performing mixed culture; and step four, after the mixed culture is finished, removing the loaches and the catfishes, filtering substances in the culture pond, collecting filter residues, drying, collecting dried substances, and using the dried substances as nutrient soil to finish the treatment of the organic garbage. Firstly, the abundant nutrient substances in the organic garbage and the strains contained in the organic garbage are utilized to promote the rapid propagation of the strains in the garbage in an oxygenation mode and improve the decomposition efficiency of the garbage, then the organic garbage is mixed with the sludge in the pond, and by the synergistic action of the microorganisms in the sludge in the pond and the microorganisms in the garbage, the garbage is degraded, and meanwhile, loaches and catfishes with strong adaptability to life are utilized to decompose the garbage, and through the adaptability of the loaches and catfishes to the environment, the regulation of living environment increases the degradation of organic garbage, and simultaneously forms micromolecular organic matters which meet the requirements of the loaches and the catfishes after being used, sedimentated in the sludge to form bottom sludge containing rich nutrient components, thereby being used as nutrient soil, thereby completing the treatment of the kitchen waste, avoiding secondary pollution and completing the reutilization of waste resources.
Preferably, the weight ratio of the water to the organic biodegradable strains in the first step is 1:500, and the cement pond is aerated every 2-3 hours in the first step, wherein the aeration flow rate is 3kg/h, and the aeration time is 20 min.
Preferably, the stirring time of the cement pool in the step two is not less than 30min, and the stirring frequency is not less than 6h per time. The mixed liquid of the organic garbage biodegradation strains and the garbage is stirred at regular time, so that the organic garbage can be fully mixed with the degradation strains, and the degradation speed is effectively accelerated.
Advantageous effects
The invention provides a biological degradation strain for organic garbage. The method has the following beneficial effects:
(1) this organic waste biodegradable bacterial through adopting and cooling to the sterile water of 40 degrees by 100 degrees plain boiled water, can carry out disinfection treatment to sterile water, effectively avoids the influence of water to follow-up bacterial cultivation, through adding water-soluble essence in bacterial crowd, can effectively reduce the stink and the peculiar smell that produce in rubbish decomposition or the fermentation to organic waste degradation process using whole bacterial. By adding the classified degradation strains into the whole strains, the whole organic garbage biodegradable strains can degrade various organic matters, and the application range of the whole strains is greatly improved. The rapid degradation grease strain is added into the whole strain, so that the whole strain can rapidly degrade grease substances. By adding aerobic and anaerobic flora, cellulase and protease into the whole organic garbage biodegradation strain, the degradation speed and the degradation rate of the whole strain on the organic garbage can be effectively improved.
(2) The organic garbage biodegradation method promotes the rapid propagation of strains in the garbage by utilizing rich nutrient substances in the organic garbage and strains contained in the organic garbage and by means of oxygenation, improves the decomposition efficiency of the garbage, then is mixed with pond silt, degrades the garbage by the synergistic action of microorganisms in the pond sludge and microorganisms in the garbage, decomposes the garbage by loaches and catfishes with strong living adaptability, regulates the living environment by the adaptability of the loaches and catfishes, increases the degradation of the organic garbage, simultaneously forms micromolecular organic matters, and settles in the silt to form bottom mud containing rich nutrient components after the use of the loaches and catfishes so as to be used as nutrient soil and further complete the treatment of residues in a cabin or a tank body, secondary pollution is avoided, and the reutilization of waste resources is completed. The mixed liquid of the organic garbage biodegradation strains and the garbage is stirred at regular time, so that the organic garbage can be fully mixed with the degradation strains, and the degradation speed is effectively accelerated.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example one
The organic garbage biodegradation strain comprises at least two or more of bacillus smithii, bacillus stearothermophilus and bacillus licheniformis with the weight portion of 20, and is characterized by also comprising the following materials with the weight portions: 10 parts of rapid oil-fat reducing strains, 20 parts of classified degrading strains, 6 parts of extracted essence, 15 parts of fouling-phagostimulant, 100 parts of water and 25 parts of basic-layer nutrient solution.
Wherein the water is sterile water cooled to 40 deg.C in 100 deg.C plain water, and the extracted essence is water soluble essence. Through adopting the sterile water that is cooled to 40 degrees by 100 degrees plain boiled water, can disinfect to sterile water, effectively avoid the influence of water to follow-up bacterial cultivation, through adding water-soluble essence in the bacterial crowd, can effectively reduce the stink and the peculiar smell that produce in rubbish decomposition or the fermentation in using whole bacterial to carry out the degradation process to organic rubbish.
The preparation method of the strain for quickly reducing the oil and fat comprises the following steps: taking a culture dish with the size of 5cm multiplied by 5cm, adding agar and the fine orange peel slices soaked by the duck blood, and uniformly paving;
adding equal mass of fluorescent bacillus, pseudomonas aeruginosa and penicillium into a culture dish, adding the culture dish, keeping the culture temperature at 30 ℃, and culturing for 2 hours; observing the surface color of the culture dish until the color becomes dark, sucking the bacterial suspension of actinomycetes and mycobacteria into the culture dish through a straw, culturing for 2h, and picking out the mould spores and hyphae on the surface to obtain the rapid oil-fat reducing strain. The rapid degradation grease strain is added into the whole strain, so that the whole strain can rapidly degrade grease substances.
A biodegradation method of organic garbage, which utilizes the organic garbage biodegradation strain to carry out degradation treatment on the organic garbage in a bin body or a tank body, and comprises the following specific steps:
step one, adding water and organic garbage biodegradable strains with the weight ratio of 1:500 into a bin body or a tank body, standing, and carrying out aeration treatment from the bottom of a cement pond at intervals of 2 hours, wherein the aeration flow is 3kg/h, and the aeration time is 20min
Step two, performing timed manual stirring or mechanical stirring on the mixture of the bin body or the tank body; stirring for 30min, stirring frequency of 6 h/time, repeating for several times, and discharging the mixed liquid in the tank or the bin to a concrete pool;
step three, adding loaches, catfishes and pond sludge into the concrete pond, and performing mixed culture;
and step four, after the mixed culture is finished, removing the loaches and the catfishes, filtering substances in the culture pond, collecting filter residues, drying, collecting dried substances, and using the dried substances as nutrient soil to finish the treatment of the organic garbage. Firstly, the abundant nutrient substances in the organic garbage and the strains contained in the organic garbage are utilized to promote the rapid propagation of the strains in the garbage in an oxygenation mode and improve the decomposition efficiency of the garbage, then the organic garbage is mixed with the sludge in the pond, and by the synergistic action of the microorganisms in the sludge in the pond and the microorganisms in the garbage, the garbage is degraded, and meanwhile, loaches and catfishes with strong adaptability to life are utilized to decompose the garbage, and through the adaptability of the loaches and catfishes to the environment, the regulation of living environment increases the degradation of organic garbage, and simultaneously forms micromolecular organic matters which meet the requirements of the loaches and the catfishes after being used, sedimentated in the sludge to form bottom sludge containing rich nutrient components, thereby being used as nutrient soil, thereby completing the treatment of the organic garbage in the tank body or the bin body, avoiding secondary pollution and completing the reutilization of waste resources.
Example two
The organic garbage biodegradation strain comprises at least two or more of bacillus smithii, bacillus stearothermophilus and bacillus licheniformis with the weight part of 25, and also comprises the following materials with the weight parts: 15 parts of rapid oil-fat reducing strains, 25 parts of classified degrading strains, 7 parts of extracted essence, 18 parts of fouling-phagostimulant, 200 parts of water and 30 parts of basic-layer nutrient solution.
Wherein the water is sterile water cooled to 40 deg.C in 100 deg.C plain water, and the extracted essence is water soluble essence. Through adopting the sterile water that is cooled to 40 degrees by 100 degrees plain boiled water, can disinfect to sterile water, effectively avoid the influence of water to follow-up bacterial cultivation, through adding water-soluble essence in the bacterial crowd, can effectively reduce the stink and the peculiar smell that produce in rubbish decomposition or the fermentation in using whole bacterial to carry out the degradation process to organic rubbish.
The preparation method of the strain for quickly reducing the oil and fat comprises the following steps: taking a culture dish with the size of 5cm multiplied by 5cm, adding agar and the fine orange peel slices soaked by the duck blood, and uniformly paving;
adding equal mass of fluorescent bacillus, pseudomonas aeruginosa and penicillium into a culture dish, adding the culture dish, keeping the culture temperature at 32 ℃, and culturing for 3 hours; observing the surface color of the culture dish until the color becomes dark, sucking the bacterial suspension of actinomycetes and mycobacteria into the culture dish through a straw, culturing for 2.5h, and picking out the mould spores and hyphae on the surface to obtain the rapid oil-fat reducing strain. The rapid degradation grease strain is added into the whole strain, so that the whole strain can rapidly degrade grease substances.
A biodegradation method of organic garbage, which utilizes the organic garbage biodegradation strain to carry out degradation treatment on the organic garbage in a bin body or a tank body, and comprises the following specific steps:
step one, adding water and organic garbage biodegradable strains with the weight ratio of 1:500 into a bin body or a tank body, standing, and carrying out aeration treatment from the bottom of a cement pond at intervals of 2.5 hours, wherein the aeration flow is 3kg/h, and the aeration time is 20min
Step two, performing timed manual stirring or mechanical stirring on the mixture of the bin body or the tank body; the stirring time of the cement pool is 40min, the stirring frequency is 7 h/time, and after the stirring is repeated for a plurality of times, the mixed liquid in the tank body or the bin is discharged into the concrete pool
Step three, adding loaches, catfishes and pond sludge into the concrete pond, and performing mixed culture;
and step four, after the mixed culture is finished, removing the loaches and the catfishes, filtering substances in the culture pond, collecting filter residues, drying, collecting dried substances, and using the dried substances as nutrient soil to finish the treatment of the organic garbage. Firstly, the abundant nutrient substances in the organic garbage and the strains contained in the organic garbage are utilized to promote the rapid propagation of the strains in the garbage in an oxygenation mode and improve the decomposition efficiency of the garbage, then the organic garbage is mixed with the sludge in the pond, and by the synergistic action of the microorganisms in the sludge in the pond and the microorganisms in the garbage, the garbage is degraded, and meanwhile, loaches and catfishes with strong adaptability to life are utilized to decompose the garbage, and through the adaptability of the loaches and catfishes to the environment, the regulation of living environment increases the degradation of organic garbage, and simultaneously forms micromolecular organic matters which meet the requirements of the loaches and the catfishes after being used, sedimentated in the sludge to form bottom sludge containing rich nutrient components, thereby being used as nutrient soil, thereby completing the treatment of the organic garbage in the tank body or the bin body, avoiding secondary pollution and completing the reutilization of waste resources.
EXAMPLE III
The organic garbage biodegradation strain comprises at least two or more of bacillus smithii, bacillus stearothermophilus and bacillus licheniformis in parts by weight of 30 parts, and also comprises the following materials in parts by weight: 15 parts of rapid oil-fat reducing strains, 30 parts of classified degrading strains, 8 parts of extracted essence, 20 parts of fouling-phagostimulant, 300 parts of water and 35 parts of basic-layer nutrient solution.
Wherein the water is sterile water cooled to 40 deg.C in 100 deg.C plain water, and the extracted essence is water soluble essence. Through adopting the sterile water that is cooled to 40 degrees by 100 degrees plain boiled water, can disinfect to sterile water, effectively avoid the influence of water to follow-up bacterial cultivation, through adding water-soluble essence in the bacterial crowd, can effectively reduce the stink and the peculiar smell that produce in rubbish decomposition or the fermentation in using whole bacterial to carry out the degradation process to organic rubbish.
The preparation method of the strain for quickly reducing the oil and fat comprises the following steps: taking a culture dish with the size of 5cm multiplied by 5cm, adding agar and the fine orange peel slices soaked by the duck blood, and uniformly paving;
adding equal mass of fluorescent bacillus, pseudomonas aeruginosa and penicillium into a culture dish, adding the culture dish, keeping the culture temperature at 35 ℃, and culturing for 3 hours; observing the surface color of the culture dish until the color becomes dark, sucking the bacterial suspension of actinomycetes and mycobacteria into the culture dish through a straw, culturing for 3h, and picking out the mould spores and hyphae on the surface to obtain the rapid oil-fat reducing strain. The rapid degradation grease strain is added into the whole strain, so that the whole strain can rapidly degrade grease substances.
A biodegradation method of organic garbage, which utilizes the organic garbage biodegradation strain to carry out degradation treatment on the organic garbage in a bin body or a tank body, and comprises the following specific steps:
step one, adding water and organic garbage biodegradable strains with the weight ratio of 1:500 into a bin body or a tank body, standing, and carrying out aeration treatment from the bottom of a cement pond at intervals of 2.5 hours, wherein the aeration flow is 3kg/h, and the aeration time is 20min
Step two, performing timed manual stirring or mechanical stirring on the mixture of the bin body or the tank body; stirring the cement pool for 30min at a stirring frequency of 7 h/time, repeating the stirring for several times, and discharging the mixed liquid in the tank or the bin into the concrete pool
Step three, adding loaches, catfishes and pond sludge into the concrete pond, and performing mixed culture;
and step four, after the mixed culture is finished, removing the loaches and the catfishes, filtering substances in the culture pond, collecting filter residues, drying, collecting dried substances, and using the dried substances as nutrient soil to finish the treatment of the organic garbage. Firstly, the abundant nutrient substances in the organic garbage and the strains contained in the organic garbage are utilized to promote the rapid propagation of the strains in the garbage in an oxygenation mode and improve the decomposition efficiency of the garbage, then the organic garbage is mixed with the sludge in the pond, and by the synergistic action of the microorganisms in the sludge in the pond and the microorganisms in the garbage, the garbage is degraded, and meanwhile, loaches and catfishes with strong adaptability to life are utilized to decompose the garbage, and through the adaptability of the loaches and catfishes to the environment, the regulation of living environment increases the degradation of organic garbage, and simultaneously forms micromolecular organic matters which meet the requirements of the loaches and the catfishes after being used, sedimentated in the sludge to form bottom sludge containing rich nutrient components, thereby being used as nutrient soil, thereby completing the treatment of the organic garbage in the tank body or the bin body, avoiding secondary pollution and completing the reutilization of waste resources.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation. The use of the phrase "comprising one of the elements does not exclude the presence of other like elements in the process, method, article, or apparatus that comprises the element.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. The organic garbage biodegradation strain comprises at least two or more of bacillus smithii, bacillus stearothermophilus and bacillus licheniformis with the weight portion of 20-30, and is characterized by also comprising the following materials with the weight portions: 10-15 parts of rapid oil-fat reducing strains, 20-30 parts of classified degrading strains, 6-8 parts of extracted essence, 15-20 parts of fouling bacteria, 100 parts of water and 300 parts of basic layer nutrient solution and 25-35 parts of basic layer nutrient solution.
2. The organic waste biodegradation species of claim 1 wherein: the water is sterile water cooled to 40 ℃ from 100 ℃ plain boiled water, and the extracted essence is water-soluble essence.
3. The organic waste biodegradation species of claim 2, wherein said species is selected from the group consisting of: the classified degradation strains specifically comprise starch strains, cellulose strains, protein strains, organic substance strains and mixed strains in equal mass ratio, and the bacteria content of the starch strains, the cellulose strains, the protein strains, the organic substance strains and the mixed strains is more than 95%.
4. The organic waste biodegradation species of claim 3, wherein said species is selected from the group consisting of: the preparation method of the strain for quickly reducing the oil and fat comprises the following steps: taking a culture dish with the size of 5cm multiplied by 5cm, adding agar and the fine orange peel slices soaked by the duck blood, and uniformly paving; adding equal mass of fluorescent bacillus, pseudomonas aeruginosa and penicillium into a culture dish, adding the culture dish, keeping the culture temperature at 30-35 ℃, and culturing for 2-4 hours; observing the surface color of the culture dish until the color becomes dark, sucking the bacterial suspension of actinomycetes and mycobacteria into the culture dish through a straw, culturing for 2-3h, and picking out the mould spores and hyphae on the surface to obtain the rapid oil-fat reducing strain.
5. The organic waste biodegradation species of claim 4 wherein: the said dirty-eating bacteria include aerobic and anaerobic flora, cellulase and protease.
6. A method for biodegrading organic waste, characterized in that organic waste in a silo or tank is treated with the organic waste biodegrading bacterial species of any one of claims 1 to 5 by:
step one, adding water and organic garbage biodegradable strains into a bin body or a tank body, standing, and carrying out oxygen aeration treatment from the bottom of the bin body or the tank body at regular time;
step two, performing timed manual stirring or mechanical stirring on the mixture of the bin body or the tank body, and discharging the mixed liquid in the tank body or the bin into a concrete water pool after repeating for a plurality of times;
step three, adding loaches, catfishes and pond sludge into the concrete pond, and performing mixed culture;
and step four, after the mixed culture is finished, removing the loaches and the catfishes, filtering substances in the culture pond, collecting filter residues, drying, collecting dried substances, and using the dried substances as nutrient soil to finish the treatment of the organic garbage.
7. The organic waste biodegradation species of claim 5, wherein said species is selected from the group consisting of: the weight ratio of the water to the organic biodegradable strain in the first step is 1:500, and the cement pond is aerated every 2-3 hours in the first step, wherein the aeration flow is 3kg/h, and the aeration time is 20 min.
8. The organic waste biodegradation species of claim 6, wherein said species is selected from the group consisting of: in the second step, the stirring time of the cement pool is not less than 30min, and the stirring frequency is not less than 6h per time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110013642.5A CN112746040A (en) | 2021-01-06 | 2021-01-06 | Organic garbage biodegradation strain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110013642.5A CN112746040A (en) | 2021-01-06 | 2021-01-06 | Organic garbage biodegradation strain |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112746040A true CN112746040A (en) | 2021-05-04 |
Family
ID=75650037
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110013642.5A Pending CN112746040A (en) | 2021-01-06 | 2021-01-06 | Organic garbage biodegradation strain |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112746040A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113600600A (en) * | 2021-08-09 | 2021-11-05 | 清华大学 | Auxiliary heat enhanced kitchen garbage rapid biological drying system and method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101306867A (en) * | 2008-07-11 | 2008-11-19 | 福州晨翔环保工程有限公司 | High-efficiency purifier of biological enzyme |
| CN102911869A (en) * | 2012-10-25 | 2013-02-06 | 王欢 | Microorganism mixed culture for degrading kitchen wastes, method for producing microorganism mixed culture for degrading kitchen wastes and method for degrading wastes through utilizing culture |
| CN108213037A (en) * | 2017-12-12 | 2018-06-29 | 常州杰轩纺织科技有限公司 | A kind of Food waste treatment method |
| CN109652322A (en) * | 2018-11-16 | 2019-04-19 | 韶关市诚湃新能源科技有限公司 | A kind of house refuse microbiological decomposer and preparation method thereof |
-
2021
- 2021-01-06 CN CN202110013642.5A patent/CN112746040A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101306867A (en) * | 2008-07-11 | 2008-11-19 | 福州晨翔环保工程有限公司 | High-efficiency purifier of biological enzyme |
| CN102911869A (en) * | 2012-10-25 | 2013-02-06 | 王欢 | Microorganism mixed culture for degrading kitchen wastes, method for producing microorganism mixed culture for degrading kitchen wastes and method for degrading wastes through utilizing culture |
| CN108213037A (en) * | 2017-12-12 | 2018-06-29 | 常州杰轩纺织科技有限公司 | A kind of Food waste treatment method |
| CN109652322A (en) * | 2018-11-16 | 2019-04-19 | 韶关市诚湃新能源科技有限公司 | A kind of house refuse microbiological decomposer and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113600600A (en) * | 2021-08-09 | 2021-11-05 | 清华大学 | Auxiliary heat enhanced kitchen garbage rapid biological drying system and method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109182193B (en) | Microbial agent and preparation method and application thereof | |
| JP5819442B2 (en) | Biological treatment method of refractory wastewater and wastewater treatment agent | |
| CN101676225B (en) | A new microbe agent and preparation method thereof | |
| CN110438024B (en) | Bacillus subtilis strain and application thereof | |
| WO2005080539A1 (en) | Cleaning agent involving application of fermentation technology and process for producing the same | |
| CN103695317B (en) | There is the production method of the efficient phosphate-solubilizing penicillium oxalicum microbial inoculum of heavy metal tolerance characteristic | |
| CN103642703B (en) | There is the production method of the efficient phosphate-solubilizing aspergillus japonicus microbial inoculum of heavy metal tolerance characteristic | |
| KR100679754B1 (en) | Method and apparatus for decomposing sludge using alkalophilic strain | |
| KR20120017710A (en) | Method to produce fertilizer using garbage | |
| US6383379B1 (en) | Method of preparing a microbial culture for wastewater treatment | |
| CN107400648A (en) | A kind of complex microorganism combines microbial inoculum, its preparation method and application, a kind of comprehensive processing method of black-odor riverway | |
| CN101045652A (en) | Comprehensive treatment method of bio-pollutant | |
| CN112746040A (en) | Organic garbage biodegradation strain | |
| CN107285482B (en) | Environment-friendly enzyme for purifying eutrophic water and preparation method thereof | |
| CN111892173A (en) | Preparation method of enzyme composite liquid and method for purifying sewer sewage and air by using enzyme composite liquid | |
| CN1340462A (en) | Bacillus subtilis method for biologically repairing culture environment of aquatic products | |
| kabashi et al. | Bio-composting process development by SSF for utilization agro-industrial wastes | |
| CN105087419A (en) | Application of bacillus subtilis in treating total nitrogen in tannery wastewater | |
| CN108358389A (en) | A kind of harmless processing of large-scale pig farm biogas slurry and utilization of resources technique | |
| CN109250883B (en) | Sludge treatment method | |
| KR20120017717A (en) | Method to produce fertilizer using garbage | |
| CN108410754B (en) | High-efficiency JM (JM) bacteria technology for treating high-salt heavy-metal degradation-resistant organic wastewater and resisting bacteria and deodorizing | |
| CN112479391A (en) | Preparation method of degradation product of well site environment-friendly toilet and method for treating water-based solid waste | |
| Govindaraj et al. | Treatment of Dairy Wastewater and Sludge Production Using Algae Bio Reactor | |
| CN110803947A (en) | Manufacturing method for producing efficient water flush fertilizer by utilizing biogas slurry |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20220221 Address after: 215000 room 103, building 4, No. 168, JINGMAO Road, Suzhou Industrial Park, Jiangsu Province Applicant after: Suzhou Dawei Environmental Protection Technology Co.,Ltd. Address before: 215000 room 2104, building 1, Xinghong building, No. 225, Suhong Middle Road, Suzhou Industrial Park, Jiangsu Province Applicant before: Jiangsu sujinyuan Biotechnology Co.,Ltd. |
|
| TA01 | Transfer of patent application right | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210504 |
|
| RJ01 | Rejection of invention patent application after publication |