CN102765817A - Biological agent formula for bioremediation management of black, stinking and eutrophication water body - Google Patents
Biological agent formula for bioremediation management of black, stinking and eutrophication water body Download PDFInfo
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- CN102765817A CN102765817A CN2012102842132A CN201210284213A CN102765817A CN 102765817 A CN102765817 A CN 102765817A CN 2012102842132 A CN2012102842132 A CN 2012102842132A CN 201210284213 A CN201210284213 A CN 201210284213A CN 102765817 A CN102765817 A CN 102765817A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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- Y02W10/10—Biological treatment of water, waste water, or sewage
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Abstract
Provided is a biological agent formula for bioremediation management of a black, stinking and eutrophication water body. The biological agent formula comprises a biological composite enzyme biological agent and a composite microorganism agent composed of a plurality of microorganisms, and proportion by weight of the biological composite enzyme biological agent and the composite microorganism agent is 20:1-1:1. When the biological agent formula is used, the biological agent is prepared according to proportion, the biological agent is added in the water body to be treated, and the biological agent formula has obvious effects on the black, stinking and eutrophication water body, is simple in operation and free of secondary pollution.
Description
Technical field
The biological prosthetic that the present invention relates to water bodys such as black smelly and eutrophication river course, lake is administered the combination formula of the required biotechnological formulation of using, and preparation of the present invention is particularly useful for the improvement of the water bodys such as the river course that discharges the black smelly and eutrophication that causes, lake because nutritive salt such as organic pollutant or nitrogen phosphorus exceed standard.
Background technology
Surface water (river course, lake) is because organic pollutant enters water body, and water surrounding is polluted.After sewage enters water body, water body is produced pollute on the one hand, water body itself has certain ability of purifying waste water on the other hand; Promptly pass through the effect of physics, chemistry with the biology of water body, make that the concentration of pollutent is minimized in the sewage, and under action of microorganisms, decompose; Thereby make water body by the unclean cleaning that reverts to; After after a while, water body often can return to contaminated preceding state, and this process is called the self-purification process of water body.But self-purification has certain limit, the maximum quantity of the pollutent that in the self-purification of waters limit, can hold, be called this water body from net capacity.When the total emission volumn of pollutent had surpassed it from net capacity, water body then can not return to original state voluntarily, and the entire environment system of water body occurs unbalance; The intravital pollutent of water increases, and causing some is that the preponderant algae of material, energy derive is bred in a large number with these pollutents, different preponderant algae populations occurs; And the various dissimilar hydrobiont kinds of related appearance is unbalance; Aquatic ecosystem is seriously damaged, and water quality deterioration causes body eutrophication.And when the self-purification capacity of water body descends, if external source is polluted and do not cut off, then the pollution load of water body will be more and more heavier; The intravital pollutent of water is also cumulative; These organic pollutants itself just need to consume the dissolved oxygen of water body, and the dissolved oxygen amount of its direct result water body also reduces along with the lifting of pollution load, after the water body dissolved oxygen amount is low to moderate 0.5mg/L; Water body begins to occur black smelly, and aerobic microbiological is replaced by anaerobion in the intravital flora advantage of water.And the intravital pollutent of water is also cumulative, and wherein a large portion exists with the form of black smelly bed mud.
Cut a dirty desilting, change public works means such as water to main at present dependence of the improvement method of the surface water pollution type of above-mentioned this type; But such governing measure exists the cost height, removing mud is prone to cause secondary pollution; Shortcoming such as cure the symptoms, not the disease also has to add that chemical reagent repairs, but to add chemical reagent only the be temporary transient improvement pollution of water body; Can not fundamentally change water quality; And adopt that to add chemical reagent improper, be easy to cause secondary pollution, the improvement of water body is had more serious consequence.And the present invention uses biological method to the black smelly and eutrophication control of water body, has adopted pure biotechnological formulation basically, safety non-pollution, and simple to operate, effect is remarkable, does not have above-mentioned shortcoming fully.
Summary of the invention
The object of the inventionBe to disclose a kind of black smelly and employed biotechnological formulation prescription of eutrophication water body biological repairing and treating; To solve because nutrition such as organic contamination and nitrogen phosphorus exceed standard the black smelly and eutrophication problem of the surface water that causes, the ecotope of recovery water body, improve the human settlement.
Technical solution of the present inventionBe:
A kind ofly be used for the biotechnological formulation prescription that black biological prosthetic smelly and eutrophication water is administered; It is characterized in that: comprise compound bio-enzyme biotechnological formulation and the complex microorganism preparations of being made up of multiple mikrobe, wherein the weight ratio of compound bio-enzyme biotechnological formulation and complex microorganism preparations is 20:1-1:1.These biotechnological formulations are with different ratios and dosing method and combine the external auxiliary measure can reach the purpose that smelly and eutrophication water are deceived in improvement.
Wherein the described compound bio-enzyme biotechnological formulation in the prescription is formed by three kinds in glycase, proteolytic enzyme, cellulase, hemicellulase, polygalacturonase, lypase and the phytase preparation, four kinds, five kinds or six kinds.Various enzyme carries out the compound bio-enzyme that mixing preparation forms according to different ratios as required, mainly is to acting on water body in a short time, carrying out the pollution load of water body and cut down rapidly.The concrete composition of various enzymes or the effect as follows:
(1) glycase is the enzyme general name of hydrolyzed starch and glycogen in the prescription, comprises AMS, beta-amylase, saccharifying enzyme and isoamylase.
AMS can cut the inner α-1 of starch chain, and the 4-glycosidic link is hydrolyzed to starch SANMALT-S, contains the oligosaccharides of 6 glucose units and has the oligosaccharides of side chain.
Beta-amylase can cut 1 from the starch molecule non reducing end, and the 4-glycosidic link generates SANMALT-S.
Saccharifying enzyme acts on the non reducing end of starch molecule, is unit with glucose, acts on the α-1 in the starch molecule successively, the oligosaccharides of 4-glycosidic link; The product that acts on after the amylose starch almost all is a glucose.
Isoamylase acts on the α-1 at pulullan molecule tapping point place, and the 6-glycosidic link is with whole side chain cutting-out the becoming amylose starch of pulullan.
(2) proteolytic enzyme in the prescription is the general name of the class of enzymes of catalytically decomposed protein peptide bond, and it acts on protein, and it is decomposed into peptone, polypeptide and total free aminoacids.
(3) cellulase in the prescription is degraded cellulose β-1, and the general name of the class of enzymes of 4-glucoside bond comprises C1 enzyme (NCE5), CX enzyme (exoglucanase) and beta-glucosidase here.
The C1 enzyme mainly acts on natural cellulose, and it is plain to be converted into the hydration amorphous cellulose; The CX enzyme is divided into CX1 enzyme and CX2 enzyme, and the CX1 enzyme is the plain enzyme of interior disconnected fiber type, it from the plain intramolecular action of hydration amorphous cellulose in β-1; The 4-glycosidic link; Generate cellodextrin and cellobiose, the CX2 enzyme is the plain enzyme of outer disconnected fiber type, and it acts on beta-glucosidase from the non reducing end of the plain molecule of hydration amorphous cellulose; Act on cellobiose, generate glucose.These enzyme synergies can thoroughly be degraded to reducing sugar-glucose with Mierocrystalline cellulose.Cellulase can crack the cell walls of fiber-enriched, nutritive substances such as its protein that comprises, starch are discharged and is used.
(4) hemicellulase in the prescription is the general name of decomposing the class of enzymes of semicellulose (comprising various pentose and the hexosans of falling).Mainly comprise beta-glucanase, Galactanase, zytase and mannase.
(5) polygalacturonase in the prescription is the common name of the enzyme of decompose pectin, and the polygalacturonase here comprises protopectinase, pectinesterase lytic enzyme, three kinds of enzymes of pectinesterase.The combined action of three kinds of enzymes can make pectin substance decompose fully.Natural pectin substance is converted to water miscible pectin under the protopectinase effect; Pectin by pectin methyl esters lytic enzyme catalysis removing methoxycarbonyl because of, generate polygalacturonase; Pectinesterase cuts off the α-1 in the pectic acid, and the 4-glycosidic link generates galacturonic acid, and galacturonic acid entering carbohydrate metabolism approach is decomposed and emits energy.
(6) lypase in the prescription is one type of ester linkage hydrolyzing enzyme with multiple catalytic capability, can the catalysis triglyceride and the reverse building-up reactions of hydrolysis, alcoholysis, esterification, transesterificationization and the ester class of some other water-insoluble ester classes.The ester bond that it mainly acts on triglyceride level makes triglyceride level be degraded to triglyceride, direactive glyceride, glycerine and lipid acid.Mainly be to administer to the reinforcement of the surface water that life oil pollution is arranged.Mainly comprise fat triglyceride lypase, hormone-sensitive lipase and monoester lypase.
(7) Sumizyme PHY in the prescription is the enzyme of catalysis phytic acid and salt hydrolysis thereof.Sumizyme PHY can be hydrolyzed into inositol and phosphoric acid salt with phytic acid and phytate.For strengthening the content of administering the intravital phosphorus of later stage degradation water effect is preferably arranged.
Described complex microorganism preparations is wherein formed by three kinds in genus bacillus, Ammonifying bacteria, nitrobacteria, denitrifying bacterium, phosphate solubilizing bacteria, polyP bacteria, milk-acid bacteria, yeast and the photosynthetic bacterium at least.Matrix and raw material that various materials that these mikrobes produce in its process of growth and secretory product thereof form mutual growth, and can form one through mutual symbiosis, propagation relation and form the microflora that complicacy, Stability Analysis of Structures, function have varied bacterium widely.
These complex microorganism preparations are to administer through the reinforcement of compound bio-enzyme at polluted-water when the after stain load descends the intravital pollutant load of water further to be reduced; Simultaneously, carry out the further reduction of the organic pollutant in the bed mud with the collocation of complex microorganism preparations and compound bio-enzyme.
Wherein, Practical situation according to the renewal rate of the pollution load of water body and water body---when the pollution load of water body the utilization compound bio-enzyme strengthen administer the back water body the eutrophication index still greater than 60 or when the renewal rate of water body too fast (water body is shorter than 5 days update time); Combine aerator that water body is carried out oxygenation as required, the aerator that is adopted is preferably web formula four-wheel aerator.
The invention has the beneficial effects as follows: can improve the microecosystem of water body to exceed standard biological prosthetic prescription that the surface water body that causes pollutes of nutritive elements such as organic contamination and nitrogen phosphorus, recover the water body species diversity, the self-purification capacity of raising water body is effectively improved water quality.Whole technology does not need desilting, and is simple, effect is remarkable, and can not cause secondary pollution.Compare with traditional improvement method, but eliminate black smelly, the eutrophication in river course, lake in the short period of time, recover the self-purification capacity in river course, lake.
Embodiment
The present invention is a kind of black smelly and employed biotechnological formulation prescription of eutrophication water body biological repairing and treating, and its embodiment is following:
(1) preparation of biotechnological formulation and addition method
Practical situation to the black smelly and eutrophication water of organic contamination; Earlier prepare compound bio-enzyme biotechnological formulation and complex microorganism preparations respectively: the compound bio-enzyme biotechnological formulation is proteolytic enzyme, cellulase, lypase and the Sumizyme PHY of 1:2.5:1:2 by mass ratio; Choose the bovine serum albumin that enzymatic protective reagent, stablizer and sanitas are 0.1% massfraction, the sodium chloride solution of 80mmol/L, the phenylformic acid of 0.05% mass concentration, be formulated as certain density compound bio-enzyme biotechnological formulation; Complex microorganism preparations: choose nitrobacteria, phosphate solubilizing bacteria, yeast and photosynthetic bacterium that mass ratio is 1:1:0.75:1.25; Be mixed with into complex microorganism preparations; Before sprinkling, be that the ratio of 1:1 is with compound bio-enzyme biotechnological formulation and complex microorganism preparations mixed preparing high-concentration biological preparation, according to the ratio preparation lower concentration biotechnological formulation of 20:1 then according to mass ratio.
Administered 1-5 days, manual work adds the biotechnological formulation of above-mentioned high density in the polluted water surface, and with pump biotechnological formulation is pumped into water bottom.Administer beginning in the 6th day; Utilization high pressure spray appliance is sprayed onto above-mentioned lower concentration biotechnological formulation in the water body; Wherein compound bio-enzyme need be with the high pressure of trying one's best with its bottom that is sprayed onto water body as much as possible to increase itself and the contacting of water body; Simultaneously, adding of compound bio-enzyme needs evenly to spray.Strengthen the pollution load of improvement water body after 30 days when water body and will cut down about 40%.
Complex microorganism preparations add not need with pressure transfer excessive, when adding, to guarantee to add in 50 centimetres of water meter simultaneously.Strengthen dosage (adding concentration is the twice in non-aeration zone) at the needs on every side that aerator is arranged.Adding for the microecosystem of stablizing water body stable of complex microorganism preparations has better action.
(2) biotechnological formulation adds effect
After surface water being carried out biological prosthetic improvement, along with the carrying out of construction, the stink of water body can be eliminated after administering 10 days; And dissolved oxygen also can progressively rise to more than the 2mg/L in about 20 days in improvement; Decrement adds biotechnological formulation subsequently, and the apparent meeting of water body about 40 days considerable change takes place, and can progressively become grey from black; Revert to normal light green at last, each item water-quality guideline of water body will progressively be increased to the above standard of face of land V class water; And the bed mud in river course, lake can progressively be degraded, water ratio can reduce greatly, bed mud gradually reduces, and the bed mud color also can progressively be reverted to light yellow by black, and final, the microecosystem in river course, lake will be stablized, and self-purification capacity is recovered.
Claims (6)
1. one kind is used for the biotechnological formulation prescription that black biological prosthetic smelly and eutrophication water is administered; It is characterized in that: comprise compound bio-enzyme biotechnological formulation and the complex microorganism preparations of being made up of multiple mikrobe, wherein the weight ratio of compound bio-enzyme biotechnological formulation and complex microorganism preparations is 20:1-1:1.
2. according to claim 1ly be used for the biotechnological formulation prescription that black biological prosthetic smelly and eutrophication water is administered; It is characterized in that described compound bio-enzyme biotechnological formulation is formed by three kinds in glycase, proteolytic enzyme, cellulase, hemicellulase, polygalacturonase, lypase and the phytase preparation, four kinds, five kinds or six kinds.
3. according to claim 1ly be used for the biotechnological formulation prescription that black biological prosthetic smelly and eutrophication water is administered; It is characterized in that described complex microorganism preparations is formed by three kinds in genus bacillus, Ammonifying bacteria, nitrobacteria, denitrifying bacterium, phosphate solubilizing bacteria, polyP bacteria, milk-acid bacteria, yeast and the photosynthetic bacterium at least.
4. an employing such as each described biotechnological formulation prescription of claim 1-3 carry out the method for water body treating; It is characterized in that: preparation obtains biotechnological formulation in proportion; Adopt high-pressure sprayer that biotechnological formulation is sprayed on the water surface of staying water, with high-pressure hydraulic pump biotechnological formulation is squeezed into the water bottom of staying water simultaneously.
5. a method as claimed in claim 4 is characterized in that: when spraying biotechnological formulation, in conjunction with aerator water body is carried out oxygenation.
6. method as claimed in claim 5, it is characterized in that: the aerator that is adopted is web formula four-wheel aerator or jet aerator.
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Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103214143A (en) * | 2013-03-21 | 2013-07-24 | 杭州天凯生物科技有限公司 | High efficiency combined restoration technology for urban river water bodies |
| CN103708694A (en) * | 2014-01-08 | 2014-04-09 | 昆山工研院华科生物高分子材料研究所有限公司 | Microorganism dredging method and device |
| CN104178433A (en) * | 2014-09-04 | 2014-12-03 | 山东省农业科学院生物技术研究中心 | Compound microbial preparation for treating urban river water pollution and preparation method of compound microbial preparation |
| CN106282061A (en) * | 2016-08-16 | 2017-01-04 | 南京中科水治理股份有限公司 | For complex microorganism preparations that black-odor riverway is administered and its preparation method and application |
| CN106587386A (en) * | 2017-02-07 | 2017-04-26 | 刘育豪 | Biological treatment method of eutrophicated water |
| CN106745828A (en) * | 2017-03-17 | 2017-05-31 | 中国科学院武汉岩土力学研究所 | A kind of broad-spectrum biological preparation administered for black and odorous water and its application method |
| CN107055806A (en) * | 2017-01-04 | 2017-08-18 | 上田环境修复股份有限公司 | A kind of complex enzyme formulation repaired for river course, lake, landscape water body and its preparation method and application |
| CN107082497A (en) * | 2017-05-22 | 2017-08-22 | 浙江阿凡柯达环保科技有限公司 | A kind of preparation method and application of polluted water body in-situ reparation sustained release enzyme bacterium mixture |
| CN107555498A (en) * | 2017-08-31 | 2018-01-09 | 航天凯天环保科技股份有限公司 | A kind of earth's surface black and odorous water and bed mud integration in situ remediation medicament and its preparation method and application |
| CN107760619A (en) * | 2017-09-30 | 2018-03-06 | 河北天泓环保科技有限公司 | Water process net carries catalytic resin enzyme body and its application method |
| CN107986452A (en) * | 2017-11-29 | 2018-05-04 | 深圳地天国际生物工程科技有限公司 | A kind of catabolic enzyme original powder and preparation method thereof |
| CN108328733A (en) * | 2018-02-11 | 2018-07-27 | 四川蓉泰环保技术有限公司 | Integrated sewage treating apparatus |
| CN108439703A (en) * | 2018-03-21 | 2018-08-24 | 南京大学 | The system and method that a kind of Plain unhurried current and the branch creek comprehensive water-body that breaks end are administered |
| CN108658404A (en) * | 2018-05-15 | 2018-10-16 | 湖南金旅环保股份有限公司 | A kind of solid union biological agent and preparation method thereof administered for black and odorous water in-situ sediment remediation |
| CN111392875A (en) * | 2020-03-25 | 2020-07-10 | 北京邦源环保科技股份有限公司 | Microbial compound enzyme for purifying river and lake sludge and application thereof |
| CN114920363A (en) * | 2022-05-13 | 2022-08-19 | 中国一冶集团有限公司 | Novel biological enzyme algaecide for river water treatment and preparation method thereof |
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| CN101811807A (en) * | 2010-05-11 | 2010-08-25 | 施怀荣 | Black and odorous and eutrophication water body biological and ecological remediation and treatment process |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101811807A (en) * | 2010-05-11 | 2010-08-25 | 施怀荣 | Black and odorous and eutrophication water body biological and ecological remediation and treatment process |
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103214143A (en) * | 2013-03-21 | 2013-07-24 | 杭州天凯生物科技有限公司 | High efficiency combined restoration technology for urban river water bodies |
| CN103708694A (en) * | 2014-01-08 | 2014-04-09 | 昆山工研院华科生物高分子材料研究所有限公司 | Microorganism dredging method and device |
| CN104178433A (en) * | 2014-09-04 | 2014-12-03 | 山东省农业科学院生物技术研究中心 | Compound microbial preparation for treating urban river water pollution and preparation method of compound microbial preparation |
| CN106282061A (en) * | 2016-08-16 | 2017-01-04 | 南京中科水治理股份有限公司 | For complex microorganism preparations that black-odor riverway is administered and its preparation method and application |
| CN107055806A (en) * | 2017-01-04 | 2017-08-18 | 上田环境修复股份有限公司 | A kind of complex enzyme formulation repaired for river course, lake, landscape water body and its preparation method and application |
| CN106587386A (en) * | 2017-02-07 | 2017-04-26 | 刘育豪 | Biological treatment method of eutrophicated water |
| CN106745828A (en) * | 2017-03-17 | 2017-05-31 | 中国科学院武汉岩土力学研究所 | A kind of broad-spectrum biological preparation administered for black and odorous water and its application method |
| CN107082497B (en) * | 2017-05-22 | 2021-06-22 | 浙江阿凡柯达环保科技有限公司 | Preparation method and application of slow-release enzyme-bacterium mixture for in-situ remediation of polluted water |
| CN107082497A (en) * | 2017-05-22 | 2017-08-22 | 浙江阿凡柯达环保科技有限公司 | A kind of preparation method and application of polluted water body in-situ reparation sustained release enzyme bacterium mixture |
| CN107555498A (en) * | 2017-08-31 | 2018-01-09 | 航天凯天环保科技股份有限公司 | A kind of earth's surface black and odorous water and bed mud integration in situ remediation medicament and its preparation method and application |
| CN107555498B (en) * | 2017-08-31 | 2020-07-10 | 航天凯天环保科技股份有限公司 | Integrated in-situ remediation and treatment agent for black and odorous water body and bottom mud on ground surface as well as preparation method and application of integrated in-situ remediation and treatment agent |
| CN107760619A (en) * | 2017-09-30 | 2018-03-06 | 河北天泓环保科技有限公司 | Water process net carries catalytic resin enzyme body and its application method |
| CN107986452A (en) * | 2017-11-29 | 2018-05-04 | 深圳地天国际生物工程科技有限公司 | A kind of catabolic enzyme original powder and preparation method thereof |
| CN108328733A (en) * | 2018-02-11 | 2018-07-27 | 四川蓉泰环保技术有限公司 | Integrated sewage treating apparatus |
| CN108439703A (en) * | 2018-03-21 | 2018-08-24 | 南京大学 | The system and method that a kind of Plain unhurried current and the branch creek comprehensive water-body that breaks end are administered |
| CN108658404A (en) * | 2018-05-15 | 2018-10-16 | 湖南金旅环保股份有限公司 | A kind of solid union biological agent and preparation method thereof administered for black and odorous water in-situ sediment remediation |
| CN111392875A (en) * | 2020-03-25 | 2020-07-10 | 北京邦源环保科技股份有限公司 | Microbial compound enzyme for purifying river and lake sludge and application thereof |
| CN114920363A (en) * | 2022-05-13 | 2022-08-19 | 中国一冶集团有限公司 | Novel biological enzyme algaecide for river water treatment and preparation method thereof |
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Application publication date: 20121107 |