CN114916552B - Compound agricultural bactericide containing penthiopyrad and application thereof - Google Patents

Compound agricultural bactericide containing penthiopyrad and application thereof Download PDF

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CN114916552B
CN114916552B CN202210528018.3A CN202210528018A CN114916552B CN 114916552 B CN114916552 B CN 114916552B CN 202210528018 A CN202210528018 A CN 202210528018A CN 114916552 B CN114916552 B CN 114916552B
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penthiopyrad
percent
agent
active component
bupirimate
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CN114916552A (en
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张小军
李彦飞
冯泽腾
盖洪光
高文兴
王国强
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SINO-AGRI LEADING BIOSCIENCES CO LTD
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SINO-AGRI LEADING BIOSCIENCES CO LTD
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/24—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing the groups, or; Thio analogues thereof
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The application discloses a compound agricultural bactericide containing penthiopyrad and application thereof. The application relates to a compound agricultural bactericidal composition, which consists of an active component A and an active component B; the active component A is penthiopyrad; the active component B is pyraclostrobin or bupirimate. The compound agricultural bactericide maintains the self-sterilization advantages of the two active components, has obvious synergistic effect, can obviously improve the control effect of treatment and protection, reduces the number of times of medication, has high safety to crops, reduces the use cost, and has the advantages of compounding the components with two different action mechanisms, causing pathogenic bacteria not to easily generate drug resistance and delaying the generation risk of drug resistance of a pathogen to a single agent.

Description

Compound agricultural bactericide containing penthiopyrad and application thereof
Technical Field
The application relates to a compound bactericide containing penthiopyrad, and belongs to the technical field of agricultural bactericides.
Background
Penthiopyrad (Penthiopyrad), chemical name: the (RS) -N- [2- (1, 3-dimethylbutyl) -3-thiaphenyl ] -1-methyl-3- (trifluoromethyl) -1H-pyrazole-4-carboxamide is a novel pyrazole amide bactericide developed by the Sanjing chemical company of Japan, is a pathogenic bacteria mitochondrial respiration inhibitor, acts on a protein complex II on a respiratory electron transfer chain of the pathogenic bacteria mitochondria, namely succinic acid dehydrogenase (SDH) or succinic acid-ubiquinone reductase (SQR), causes tricarboxylic acid circulation disorder, blocks energy metabolism, inhibits growth of pathogenic bacteria, and causes death of the pathogenic bacteria, thereby achieving the purpose of preventing and treating diseases (SDHI bactericides are named because of the action mechanism).
The penthiopyrad has different sterilization spectrums with the existing carboxamide bactericides, and is mainly applied to field crops such as rape, peanut, sunflower, potato, soybean and the like, and also applied to markets such as fruit trees, vegetables and the like; preventing and treating various diseases such as fusarium head blight, powdery mildew, gray mold, leaf spot, brown rot, rhizoctonia, sclerotinia sclerotiorum and the like, and ensuring safety to crops; and are also recommended for controlling gray mold and powdery mildew, which are resistant to other bactericides.
Pyraclostrobin is one kind of broad spectrum methoxy acrylic acid sterilizing agent and is used mainly in preventing and controlling various diseases caused by fungi in crop. Bupirimate is a super-strong systemic pyrimidine bactericide, and has special effects on powdery mildew of various crops. However, these two pesticides are applied continuously in high dosage for a long period of time, which is liable to cause problems such as residue of the pesticide, environmental pollution and development of drug-resistant fungi. Therefore, the reasonable chemical bactericide is selected for compounding or blending, has the positive characteristics of expanding the bactericidal spectrum, improving the control effect, prolonging the application period, reducing the dosage, reducing the phytotoxicity, reducing the residue, delaying the occurrence and development of the drug resistance of fungi and the drug resistance, and the like, and the bactericide compounding is one of the most effective methods for solving the problems. In addition, the price of developing new bactericides is continuously increased, and in contrast, the development and research of efficient, low-toxicity and low-residue compound and mixed bactericides has the advantages of low investment and short development period, and is valued at home and abroad, and various enterprises increase the development strength in a dispute.
In the actual process of agricultural production, the most easily generated problem of disease control is the generation of disease resistance. The compounding of different varieties of components is a common method for preventing and treating resistant diseases. Different components are compounded, and whether a certain compounding is synergistic, additive or antagonistic is judged according to the actual application effect. Under most conditions, the sterilizing compound effect is a summation effect, the actual compound with the synergy is few, and especially the compound with obvious synergy and high synergy ratio is less.
Disclosure of Invention
Based on the above circumstances, the application aims to provide a compound bactericide containing penthiopyrad, which is prepared by compounding two bactericides with different structures, has the advantages of obvious synergistic effect, low use cost, excellent control effect, no resistance and the like, and is mainly applied to the control of gray mold and powdery mildew of crops.
In order to achieve the above purpose, the technical scheme adopted by the application is as follows:
in a first aspect, the application provides a compound agricultural sterilization composition, which consists of an active component A and an active component B;
the active component A is penthiopyrad;
the active component B is pyraclostrobin or bupirimate.
In the compound agricultural bactericidal composition, the mass ratio of the active component A to the active component B is 1:0.1 to 1:50, e.g. 1:0.5 to 1:2 or 1:0.1 to 1:20.
preferably, the mass ratio of the active component A to the active component B is 1:0.2 to 1:5.
further preferably, the active component B is pyraclostrobin; the mass ratio of the active component A to the active component B is 1:1.5.
further preferably, the active component B is bupirimate; the mass ratio of the active component A to the active component B is 1:1.
in a second aspect, the application provides the use of the compound agricultural bactericidal composition of any one of the above-mentioned aspects for controlling gray mold and/or powdery mildew of crops.
In a third aspect, the application provides a compound agricultural bactericide, which is prepared from the compound agricultural bactericidal composition and auxiliary materials acceptable in pesticide preparations, wherein the mass percentage of the compound agricultural bactericidal composition is 1-90%. Preferably, the mass percentage of the compound agricultural bactericidal composition is 20-50%, such as 30%, 29% or 32%.
Further, the agricultural sterilization composition can be prepared into any formulation which can be applied to agriculture by adding an auxiliary agent and an excipient according to the actual production requirement, and preferably, the formulation of the compound agricultural sterilization composition can be any one of suspending agent, aqueous emulsion, dispersible oil suspending agent, wettable powder, water dispersible granule, emulsifiable concentrate and microemulsion.
The compound agricultural bactericide is a suspending agent and comprises the following components in percentage by mass: 1 to 40 percent of penthiopyrad, 1 to 40 percent of active component B, 2 to 10 percent of dispersing agent, 1 to 5 percent of wetting agent, 2 to 10 percent of antifreezing agent, 0.1 to 1 percent of defoaming agent, 0.1 to 3 percent of thickening agent, 0.1 to 2 percent of preservative and the balance of water;
preferably, the composition comprises the following components in percentage by mass: 1 to 20 percent of penthiopyrad, 1 to 20 percent of component B, 2 to 6 percent of dispersing agent, 1 to 2 percent of wetting agent, 2 to 5 percent of antifreezing agent, 0.1 to 0.2 percent of defoaming agent, 0.1 to 0.2 percent of thickening agent, 0.1 to 0.2 percent of preservative and the balance of water;
as an example, it consists of the following components in mass percent: 12% of penthiopyrad, 18% of component B, 3% of dispersing agent, 1% of wetting agent, 5% of antifreezing agent, 0.5% of defoaming agent, 0.1% of thickening agent, 0.1% of preservative and the balance of water.
The compound agricultural bactericide is an aqueous emulsion and comprises the following components in percentage by mass: 1 to 40 percent of penthiopyrad, 1 to 40 percent of component B, 2 to 10 percent of dispersing agent, 1 to 5 percent of emulsifying agent, 10 to 30 percent of organic solvent, 2 to 10 percent of antifreezing agent, 0.1 to 1 percent of defoaming agent, 0.1 to 3 percent of thickening agent, 0.1 to 2 percent of preservative and the balance of water;
preferably, the composition comprises the following components in percentage by mass: 1 to 20 percent of penthiopyrad, 1 to 20 percent of component B, 2 to 10 percent of dispersing agent, 1 to 3 percent of emulsifying agent, 10 to 15 percent of organic solvent, 2 to 5 percent of antifreezing agent, 0.1 to 0.2 percent of defoaming agent, 0.1 to 0.2 percent of thickening agent, 0.1 to 0.2 percent of preservative and the balance of water;
as an example, it consists of the following components in mass percent: 14.5% of penthiopyrad, 14.5% of component B, 3% of dispersing agent, 3% of emulsifying agent, 15% of organic solvent, 5% of antifreezing agent, 0.2% of defoaming agent, 0.2% of thickening agent, 0.2% of preservative and the balance of water; or alternatively, the first and second heat exchangers may be,
the coating comprises the following components in percentage by mass: 25% of penthiopyrad, 5% of component B, 3% of dispersing agent, 3% of emulsifying agent, 20% of organic solvent, 5% of antifreezing agent, 0.2% of defoaming agent, 0.2% of thickening agent, 0.2% of preservative and the balance of water.
The compound agricultural bactericide is a dispersible oil suspending agent and comprises the following components in percentage by mass: 1 to 40 percent of penthiopyrad, 1 to 40 percent of component B, 1 to 5 percent of dispersing agent, 5 to 20 percent of emulsifying agent, 1 to 5 percent of thickening agent and the balance of oil medium;
preferably, the composition comprises the following components in percentage by mass: 1 to 20 percent of penthiopyrad, 1 to 20 percent of component B, 1 to 5 percent of dispersing agent, 5 to 15 percent of emulsifying agent, 1 to 5 percent of thickening agent and the balance of oil medium;
as an example, it consists of the following components in mass percent: 17.5% of penthiopyrad, 12.5% of component B, 1% of dispersing agent, 6% of emulsifying agent, 2% of thickening agent and the balance of oil medium; or alternatively, the first and second heat exchangers may be,
the coating comprises the following components in percentage by mass: 20% of penthiopyrad, 10% of component B, 2% of dispersing agent, 5% of emulsifying agent, 3% of thickening agent and the balance of oil medium.
The compound agricultural bactericide is wettable powder and consists of the following components in percentage by mass: 1 to 40 percent of penthiopyrad, 1 to 40 percent of component B, 3 to 10 percent of dispersing agent, 1 to 5 percent of wetting agent and the balance of filler;
preferably, the composition comprises the following components in percentage by mass: 1 to 20 percent of penthiopyrad, 1 to 20 percent of component B, 5 to 10 percent of dispersing agent, 1 to 5 percent of wetting agent and the balance of filler;
as an example, it consists of the following components in mass percent: 10% of penthiopyrad, 20% of component B, 7% of dispersing agent, 3% of wetting agent and the balance of filler.
The compound agricultural bactericide is water dispersible granules and comprises the following components in percentage by mass: 1 to 40 percent of penthiopyrad, 1 to 40 percent of component B, 1 to 10 percent of dispersing agent, 1 to 5 percent of wetting agent and the balance of filler;
preferably, the composition comprises the following components in percentage by mass: 1 to 20 percent of penthiopyrad, 1 to 20 percent of component B, 1 to 10 percent of dispersing agent, 1 to 5 percent of wetting agent and the balance of filler;
as an example, it consists of the following components in mass percent: 24% of penthiopyrad, 8% of component B, 7% of dispersing agent, 4% of wetting agent and the balance of filler; or alternatively, the first and second heat exchangers may be,
the coating comprises the following components in percentage by mass: 15% of penthiopyrad, 7% of component B, 7% of dispersing agent, 4% of wetting agent and the balance of filler.
The compound agricultural bactericide is missible oil, and consists of the following components in percentage by mass: 1 to 40 percent of penthiopyrad, 1 to 40 percent of component B, 1 to 10 percent of emulsifying agent and the balance of organic solvent;
preferably, the composition comprises the following components in percentage by mass: 1 to 20 percent of penthiopyrad, 1 to 20 percent of component B, 1 to 10 percent of emulsifying agent and the balance of organic solvent.
The compound agricultural bactericide is microemulsion and comprises the following components in percentage by mass: 1 to 40 percent of penthiopyrad, 1 to 40 percent of component B, 5 to 25 percent of organic solvent, 15 to 25 percent of emulsifier and the balance of water;
preferably, the composition comprises the following components in percentage by mass: 1 to 20 percent of penthiopyrad, 1 to 20 percent of component B, 5 to 25 percent of organic solvent, 15 to 25 percent of emulsifier and the balance of water;
as an example, it consists of the following components in mass percent: 15% of penthiopyrad, 15% of component B, 25% of organic solvent, 20% of emulsifier and the balance of water.
The auxiliary agents in the preparation are as follows:
the dispersing agent is selected from one or more than two of naphthalene formaldehyde condensate sulfonate, alkyl naphthalene formaldehyde condensate sulfonate, fatty alcohol ethylene oxide adduct sulfonate, alkylphenol polyoxyethylene ether sulfonate, lignin and derivative sulfonate thereof, polymerized alkylaryl sulfonate, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, fatty alcohol polyoxyethylene ether sulfate, fatty alcohol ethane adduct phosphate, alkylphenol polyoxyethylene phosphate, polyacrylate, copolymer salt (or ester) of polyacrylic acid and maleic anhydride, EO-PO block copolymer, hydroxy stearic acid and alkyl glycol block copolymer.
The wetting agent is selected from one or a mixture of more than two of sodium dodecyl sulfate, sodium dodecyl benzene sulfonate, sodium triisopropyl naphthalene sulfonate, sodium diisobutyl naphthalene sulfonate, dioctyl sodium succinate, fatty alcohol polyoxyethylene ether sulfate, alkylphenol polyoxyethylene ether sulfosuccinate and fatty alcohol polyoxyethylene ether (normal or isomeric).
The emulsifier is selected from one or more than two of alkylphenol ethoxylates, benzyl phenol ethoxylates, phenethyl phenol ethoxylate phosphate, fatty alcohol ethoxylates, phenethyl phenol ethoxylate polyoxypropylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate formaldehyde condensate, polyoxyethylene sorbitan monooleate, sorbitan fatty acid ester, polyethylene glycol oleate, sorbitol polyoxyethylene ether oleate and dodecylbenzene sulfonate (calcium salt or isopropylamine salt).
The antifreezing agent is selected from one or a mixture of more than two of ethylene glycol, propylene glycol, glycerol, polyethylene glycol, urea, sorbitol and inorganic salt.
The defoamer is selected from one or a mixture of more than two of organic silicone, aliphatic alcohol with 8-10 carbon atoms, saturated aliphatic carboxylic acid with 19-20 carbon atoms and ester, ester and ether compounds.
The thickener is selected from one or a mixture of more than two of xanthan gum, acacia, methylcellulose, sodium carboxymethylcellulose, hydroxypropyl cellulose, sodium acrylate, polyvinyl alcohol, polyvinylpyrrolidone, sodium polyacrylate, polyvinyl acetate, dispersible silicic acid, fumed silica, organic bentonite and magnesium aluminum silicate.
The preservative is selected from one or a mixture of more than two of sodium benzoate, sorbic acid, sodium salicylate, pinus koraiensis, benziothiazolinone and phenoxyethanol.
The organic solvent is selected from one or a mixture of more than two of cyclohexanone, N-dimethylformamide, N-dimethyldecanoamide, N-dimethyloctadecanoamide, N-methylpyrrolidone, N-octylpyrrolidone, dibasic ester and aromatic hydrocarbon solvent oil.
The oil medium is one or more of soybean oil, rapeseed oil, cotton seed oil, corn oil, castor oil, palm oil, epoxidized soybean oil, diesel oil, methyl esterified vegetable oil, engine oil and mineral oil.
The filler is selected from one or a mixture of more than two of diatomite, attapulgite, light calcium carbonate, sepiolite, kaolin, white carbon black, talcum powder, montmorillonite, corn starch and ammonium sulfate.
In a fourth aspect, the application provides the use of the compound agricultural fungicide of any one of the above aspects in controlling gray mold or powdery mildew of crops.
In the above application, the crop may be strawberry, melon or tomato.
The compound agricultural bactericide is applied to crops or crop parts by spraying, root irrigation or seed dressing.
The inventor has long-term research and found on the basis of a large number of experiments that the compound of the penthiopyrad and the pyraclostrobin is used for controlling gray mold of crops, the compound of the penthiopyrad and the bupirimate is used for controlling powdery mildew of crops, and the compound of the penthiopyrad and the bupirimate has obvious synergism compared with the corresponding single dose and has no resistance. In addition, the report related to the compounding of penthiopyrad and pyraclostrobin or bupirimate has little disclosure, and the application has very important significance for expanding the application of the compound product containing penthiopyrad.
Compared with the prior art, the beneficial effects are that:
the application provides a compound bactericidal composition containing penthiopyrad, which is compounded by penthiopyrad and one of pyraclostrobin or bupirimate, maintains the self bactericidal advantages of two active components, has obvious synergistic action, can obviously improve the control effect of treatment and protection, reduces the number of times of drug use, has high safety to crops, reduces the use cost, and is difficult to generate drug resistance for pathogenic bacteria and delays the generation risk of drug resistance of the pathogenic matters to single agents due to the composition of two different action mechanisms.
Detailed Description
The following detailed description of the application is provided in connection with the accompanying drawings that are presented to illustrate the application and not to limit the scope thereof. The examples provided below are intended as guidelines for further modifications by one of ordinary skill in the art and are not to be construed as limiting the application in any way.
The experimental methods used in the following examples are all conventional methods unless otherwise specified; the materials, reagents and the like used, unless otherwise specified, are all commercially available.
Examples of biological Activity
The embodiment is used for explaining the synergistic effect of the penthiopyrad-containing compound bactericidal composition.
The inventor carries out synergism analysis of different proportions of binary compound bactericide compositions of penthiopyrad, pyraclostrobin and bupirimate by a large number of screening tests by using a grand cloud Peel method, and discovers that the penthiopyrad and pyraclostrobin composition has obvious synergism on gray mold of crops in a certain proportion range; the composition of the penthiopyrad and bupirimate has obvious synergistic effect on powdery mildew of crops. This synergy is not a simple addition of the two agents, and is specifically illustrated by the bioassay examples below.
Biological Activity example 1, compounding penthiopyrad with pyraclostrobin was used for the in-house bioassay of strawberry gray mold.
Test strain: the botrytis cinerea (Botrytis cinerea) is obtained from a strawberry planting greenhouse in Nanchang city by separation and purification (Zhang Chuanbo, easy germination, sun Yunzi, and the like), and the indoor toxicity of several bactericides and compound agents thereof on the botrytis cinerea is measured [ J ]. Hubei agricultural science, 2013,52 (14): 3299-3301+3305.).
Test agent: 99.5% of penthiopyrad original drug and 97.5% of pyraclostrobin original drug. The raw materials are provided by Zhongnonghua biotechnology Co., ltd.
The test method comprises the following steps:
a plate mycelium growth inhibition method is adopted. Mother liquor of penthiopyrad and pyraclostrobin was prepared at 2500. Mu.g/mL with dimethyl sulfoxide at 50mL each, and the mother liquor was diluted with 0.05% Tween-80 water. On the basis of the preliminary experiments, the following is carried out: 1,1.5:1,1:1,1:1.5,1:2 to finally prepare the drug-containing PDA culture medium with the concentration gradients of 1, 5, 10, 20 and 30 mug/mL of single medicament and mixed medicament.
The activity of penthiopyrad and pyraclostrobin on the gray mold of the strawberry is determined by a growth rate method, under the aseptic operation condition, bacterial cakes with the diameter of 5mm are taken from the colony edge of the gray mold of the strawberry by a sterilization puncher, the mycelia are inoculated on a medicated PDA culture medium downwards, one bacterial cake is cultivated in an inverted way at the temperature of 25 ℃, and each treatment is repeated for 3 times, and a plate containing dimethyl sulfoxide is used as a control. When the hypha growth diameter of the control group reaches about 50% of the diameter of the culture dish, the colony diameter is measured by a crisscross method, and the (relative) bacteriostasis rate is calculated. With the help of SPSS software, the logarithmic value of the concentration of the medicament is taken as an abscissa (x), the value of the rate of inhibiting hypha growth is taken as an ordinate (y), and a virulence regression equation and EC are obtained 50 And a correlation coefficient, calculating a synergistic coefficient (SR) of the mixture according to the Wadley method, and evaluating the combined action type of the mixture. See table 1.
Table 1: penthiopyrad and pyraclostrobin and their combination are used for indoor toxicity determination of strawberry gray mold
As can be seen from the test results in Table 1, the 99.5% penthiopyrad technical has more remarkable effect of inhibiting the growth of Botrytis cinerea compared with the 97.5% pyraclostrobin technical. Pithiabendazole and pyraclostrobinThe 5 different mixed combinations of the esters have good inhibition effect on the botrytis cinerea of the strawberries and ideal activity. Wherein the penthiopyrad and pyraclostrobin are mixed according to the weight ratio of 1:1.5 compounding combinations of EC 50 13.93 mug/mL, the synergy coefficient SR=1.65, the inhibition effect is better than that of two single agents, and the synergy is most obvious; EC of other 4 ratios 50 The addition was found to be 13.15. Mu.g/mL, 16.99. Mu.g/mL, 19.29. Mu.g/mL, and 16.78. Mu.g/mL, respectively.
Bioactive example 2, penthiopyrad and bupirimate were compounded for in-house bioassay of strawberry powdery mildew.
Test agent:
99.5% of penthiopyrad original drug and 95.2% of bupirimate original drug. The raw materials are provided by Zhongnonghua biotechnology Co., ltd.
Test target
From the collection and separation of non-applied penthiopyrad and bupirimate from the same type of strawberry potting, the test strawberry variety: and (3) flanged ground.
The test method comprises the following steps:
firstly, dissolving raw medicines with acetone, preparing proper amounts of the two raw medicines into different proportions according to the result of a preliminary experiment, and then diluting each treatment into a plurality of concentration gradients for standby. The method comprises the steps of cultivating a disease-sensitive strawberry variety in a seedling pot in a flange manner, placing the strawberry variety in a greenhouse for cultivation, and collecting leaves with the same leaf age after plants enter a flowering phase for cultivation and measurement of powdery mildew of the strawberry.
Culture of strawberry powdery mildew and preparation of spore suspension
The powdery mildew of strawberry is cultivated by a living plant method under the conditions of 20 ℃ and 12h light-dark alternation, and is cultivated for 1 time every 30 days. Eluting conidium on diseased leaf with sterile water during inoculation to obtain a spore concentration of 1×10 6 individual/mL of suspension.
Test strain sensitivity assay
Toxicity was determined by a She Diebao wet method. Firstly preparing the collected leaves into leaf discs with the diameter of 1.5cm, randomly mixing, respectively soaking in prepared series of concentrated liquid medicine for lh, and each concentrated liquid medicineThe test uses the treatment without adding the medicament as blank control, the front surface of the leaf is placed on the water absorbing paper wetted by the same liquid medicine concentration after the soaking is finished, the liquid medicine on the leaf disk is sucked dry, 10L of prepared spore suspension is inoculated in the center of the leaf disk, the leaf disk is placed for 5min at room temperature, and is cultured under the condition of 20 ℃ and 12h light and dark alternation, the disease area on the leaf disk is measured after 10 days, and the EC is calculated 50 。
Based on the preliminary test, the toxicity of single-dose penthiopyrad and bupirimate are measured by the method, and EC of the single-dose penthiopyrad and bupirimate is measured 50 The values were 7.213mg/L and 7.840mg/L, respectively.
Dividing disease grade according to the percentage of disease spot area to leaf disc area
Level 0: no disease;
stage 1: the area of the spore pile is less than 5% of the whole leaf area;
3 stages: the area of the spore pile accounts for less than 6% -10% of the whole leaf area;
5 stages: the area of the spore pile accounts for 11% -20% of the whole leaf area;
7 stages: the area of the spore pile accounts for 21% -50% of the whole leaf area;
stage 9: the area of the spore pile accounts for more than 50% of the whole leaf area.
Mixed agent toxicity determination and result analysis
The toxicity of the mixture is measured according to the mixture ratio by a single-dose toxicity measuring method.
If the control mortality is less than 5%, the correction is not performed, and the control mortality is between 5% and 20%, the correction is performed according to a formula, and the control mortality is more than 20%, and the test is reworked.
The logarithmic value of the concentration (mg/L) of the medicament is taken as an independent variable x, the several value of the corrected mortality is taken as a dependent variable y, virulence regression equations are respectively established, and DPS software is adopted to calculate the EC of the single medicament and each mixed medicament 50 Co-toxicity coefficients (CTCs) were calculated according to the grand cloud Pei method. The co-toxicity coefficient CTC is calculated as follows: (taking penthiopyrad as a standard medicament, the toxicity index of the penthiopyrad is 100):
toxicity index of bupirimate (TI) =ec of penthiopyrad 50 Eb/bEC of phenolsulfonate 50 ×100；
True virulence index (ATI) =ec of penthiopyrad for M 50 EC of/M 50 ×100；
Theoretical virulence index (TTI) =ti×p penthiopyrad+ti×p bupirimate of penthiopyrad;
co-toxicity coefficient of M (CTC) =ati of M/tti×100 of M;
wherein:
m is a mixture of penthiopyrad and bupirimate in different proportions;
the P bupirimate is the proportion of bupirimate in the mixture;
the P-penthiopyrad is the proportion of the penthiopyrad in the mixture.
The virulence measurement results are as follows:
table 2: indoor toxicity measurement result of penthiopyrad and bupirimate combination on powdery mildew
The indoor toxicity measurement result shows that: the pyrithione and bupirimate have better control effect on strawberry powdery mildew pathogenic bacteria when being mixed, especially when the pyrithione is mixed with bupirimate: bupirimate with mass ratio of 24:24, i.e. 1: the synergy is most remarkable in 1.
Formulation examples
Formulation example 1, 30% penthiopyrad pyraclostrobin suspension
12% of penthiopyrad, 18% of pyraclostrobin, 3% of dispersing agent Morwet D-425 (alkyl naphthalene formaldehyde condensate sulfonate, akzo Nobel), 1% of wetting agent Genapol X080 (isomeric alcohol polyoxyethylene ether, solvay), 5% of 1, 2-propanediol (antifreezing agent, shandong national chemical Co., ltd.), 0.1% of xanthan gum (thickening agent, hebei Pengyu Biotech Co., ltd.), 0.1% of kasong (preservative, beijing Guangdong Yinong Chemie Co., ltd.), 0.5% of SAG 1522 (organosilicon defoamer, michaelis high new material group) and 100% of deionized water (self-made) are weighed according to mass percentage. The raw materials are mixed, sanded by a sand mill until the particle size is smaller than 5 mu m, and the 30% penthiopyrad pyraclostrobin suspending agent is obtained after filtration.
Formulation example 2, 30% penthiopyrad pyraclostrobin wettable powder
Weighing 10% of penthiopyrad, 20% of pyraclostrobin and dispersing agent according to mass percentNN8906 (naphthalene formaldehyde condensate sulfonate, BASF) 7%, a wetting agent Geropon SDS (dioctyl sodium sulfosuccinate, solvay) 3%, filler SIPERNAT 22LS (white carbon black, EVONIK) 7%, and filler Celite 499 (diatomaceous earth, imerrys) make up 100%. The raw materials are mixed, crushed by an airflow crusher and uniformly mixed, and the 30% penthiopyrad pyraclostrobin wettable powder is obtained.
Formulation example 3, 30% penthiopyrad pyraclostrobin water dispersible granule
15% of penthiopyrad, 15% of pyraclostrobin, 7% of a dispersing agent Suparex K powder (naphthalene formaldehyde condensate sulfonate dispersing agent, switzerland high chemical company), 4% of wetting agent sodium dodecyl sulfate (Toming Co., ltd.), 20% of corn starch (filler, lin Yitailong Utility Co., ltd.) and the filler MXK (calcined kaolin, inner Mongolin Kaolin Co., ltd.) are weighed according to the mass percentage. The raw materials are mixed, crushed by an airflow crusher, uniformly mixed, kneaded, and granulated, dried and screened to obtain the 30% penthiopyrad pyraclostrobin water dispersible granule.
Formulation example 4, 30% penthiopyrad pyraclostrobin dispersible oil suspension
According to mass percentage, 20 percent of penthiopyrad, 10 percent of pyraclostrobin, 6 percent of emulsifier Emulsogen EL360 (castor oil polyoxyethylene ether, clariant), 5 percent of emulsifier Phenylsulfonat CAL (calcium dodecyl benzene sulfonate, clariant), 2 percent of dispersant Atlox Metasperse 550S (polyacrylate, croda), 3 percent of thickener ODgel-04 (organic soil, zhejiang Feng Hong New Material Co., ltd.) and 100 percent of dispersion medium methyl oleate 0295 (Suzhou FengBei Biotechnology Co., ltd.) are weighed. The raw materials are mixed, sanded by a sand mill until the particle size is smaller than 5 mu m, and filtered to obtain the 30% penthiopyrad pyraclostrobin dispersible oil suspending agent.
Formulation example 5, 29% penthiopyrad pyraclostrobin aqueous emulsion
According to mass percentage, 14.5% of penthiopyrad, 14.5% of pyraclostrobin, 15% of solvent oil S-150# (Jiangsu Hua Lun chemical Co., ltd.), 3% of dispersing agent Soprophor SC (alkylphenol ethoxylate, solvay), 3% of emulsifying agent EL-40 (castor oil polyoxyethylene ether, jiangsu sea Androme petrochemical Co., ltd.), 5% of antifreezing agent urea (chemical fertilizer control company, gmbH), 0.2% of antifoaming agent Sag 630 (organic silicone, michaelis high new material group), 0.2% of thickening agent xanthan gum (Shanghai Mo Hua trade Co., ltd.), 0.2% of preservative Kathon (Beijing Guangdong Yinong chemical Co., ltd.) and 100% of water are weighed. Firstly, the penthiopyrad and the pyraclostrobin are dissolved in solvent oil to form an oil phase, then the rest components are mixed with water to form a water phase, finally, the oil phase is slowly added into the water phase sheared at high speed in batches, and the 29% penthiopyrad pyraclostrobin aqueous emulsion is obtained through homogenizing, emulsifying and filtering.
Formulation example 6, 30% penthiopyrad bupirimate dispersible oil suspension
17.5% of penthiopyrad, 12.5% of bupirimate, 6% of emulsifier Emulsogen TS200 (phenethyl phenol polyoxyethylene ether phosphate, clariant), 6% of emulsifier Zephrym 3300B (dodecylbenzene sulfonic acid isopropyl amine salt, croda) 6%, 1% of dispersant YUS-EP60P (alkylphenol polyoxyethylene ether sulfosuccinate, TAKEMOTO), 2% of thickener AEROSIL R974 (white carbon black, EVONIK) and 100% of soybean oil (Yihai Jia Rib group) are weighed according to mass percentage. The raw materials are mixed, sanded by a sand mill until the particle size is smaller than 5 mu m, and filtered to obtain the 30% penthiopyrad ethirimol sulfonate dispersible oil suspending agent.
Formulation example 7, 30% aqueous emulsion of penthiopyrad bupirimate
According to mass percentage, weighing 25% of penthiopyrad, 5% of bupirimate, 20% of solvent oil S-200# (Jiangsu Huaren chemical Co., ltd.), 3% of dispersing agent TERSPERSE2020 (alkyl naphthalene sulfonate, huntsman), 3% of emulsifying agent ATLAS G-1086 (sorbitol polyoxyethylene ether oleate, croda) 3%, 5% of antifreezing agent urea (medium chemical fertilizer control Co., ltd.), 0.2% of antifoaming agent Sag 630 (organic silicone, michaku high new material group), 0.2% of thickening agent xanthan gum (Shanghai Mo Hua engineering Co., ltd.), 0.2% of preservative Kathon (Beijing Guangdong Yinong chemical Co., ltd.) and 100% of water. Firstly, the penthiopyrad and bupirimate are dissolved in solvent oil to form an oil phase, then the rest components are mixed with water to form a water phase, finally, the oil phase is slowly added into the water phase sheared at high speed in batches, and the mixture is homogenized, emulsified and filtered to obtain 30% penthiopyrad bupirimate aqueous emulsion.
Formulation example 8, 30% penthiopyrad bupirimate microemulsion
15% of penthiopyrad, 15% of bupirimate, 25% of solvent N, N-dimethyl octanamide (Hubei Chengfeng chemical Co., ltd.) and 20% of emulsifier Tween-20 (polyoxyethylene sorbitan monooleate, jiangsu sea-Ann petrochemical plant) are weighed according to the mass percentage, and the water is filled up to 100%. Dissolving penthiopyrad and bupirimate in an organic solvent, adding an emulsifying agent, stirring uniformly, and finally adding water, stirring uniformly to obtain the 30% penthiopyrad bupirimate microemulsion.
Formulation example 9, 30% penthiopyrad bupirimate wettable powder
20% of penthiopyrad, 10% of bupirimate, 7% of dispersing agent TERSPERSE2020 (alkyl naphthalene sulfonate, huntsman), 3% of wetting agent Geropon SDS (dioctyl sodium sulfonate, solvay), 8% of filler SIPERNAT 22LS (white carbon black, EVONIK), and 100% of filler MXK (calcined kaolin, inner Mongolian kaolin Co.) are weighed according to mass percentage. The raw materials are mixed, crushed by an air flow crusher and uniformly mixed to obtain 30% penthiopyrad bupirimate wettable powder.
Formulation example 10, 32% penthiopyrad bupirimate water dispersible granule
24% of penthiopyrad, 8% of bupirimate, 7% of a dispersing agent SUPRAGIL GN (naphthalene formaldehyde condensate sulfonate dispersing agent, solvay), 4% of a wetting agent sodium dodecyl sulfate (Toming club evolutionary Co., ltd.), 20% of filler ammonium sulfate (Shandong wars biotechnology Co., ltd.) and the filler MXK (calcined kaolin, inner Mongolian Kaolin Co., ltd.) are weighed according to the mass percentage. The raw materials are mixed, crushed by an airflow crusher, uniformly mixed, kneaded, and granulated, dried and screened to obtain the 32% penthiopyrad pyraclostrobin water dispersible granule.
Example of field efficacy
Example of field efficacy 1, example of field efficacy of Gray mold of strawberry
Test agent: selecting a mixed agent of penthiopyrad and pyraclostrobin prepared in preparation examples 1-5;
20% penthiopyrad suspension (AGRO, sanwell chemical Co., ltd.);
25% pyraclostrobin suspension (Shandong Kang Qiao Biotechnology Co., ltd.);
the test is carried out on a strawberry base in a full city of Hebei baoding to verify the control effect of each mixed pesticide on the gray mold of the strawberry. The test crop is strawberry, and the variety is Chaptera crassifolia. The test agent and the dosage are shown in Table 3, the cell treatment of the test agent, the control agent and the blank control adopts random block arrangement, and the cell area is 30m 2 The disease number is investigated before the drug administration for 4 times, the drug administration is carried out twice, and the control effect is investigated before each drug administration and 7 days and 15 days after the last drug administration. Four-point investigation is randomly carried out in each cell, two plants are investigated in each spot, and all leaves are investigated in each plant. The disease index was calculated and the results are shown in Table 3.
Classification method (in units of leaves) to
Grade 0: no lesions;
grade 1, wherein the area of the disease spots accounts for less than 5% of the whole leaf area;
grade 3, wherein the area of the disease spots accounts for 6% -10% of the whole leaf area;
grade 5, wherein the area of the disease spots accounts for 11% -25% of the whole leaf area;
7 grades, wherein the area of the disease spots accounts for 26% -50% of the whole leaf area;
grade 9, the area of the disease spots accounts for more than 50% of the whole leaf area.
The calculation method of the drug effect is that
Safety investigation and impact on other organisms: the effect of each treatment of the agent on strawberry growth and on other diseases was observed on the first day and several days after each spray.
Table 3 results of field efficacy experiments for controlling strawberry gray mold by penthiopyrad and pyraclostrobin mixture
Safety investigation of strawberries shows that each treatment agent does not cause phytotoxicity to strawberry stems and leaves during the test period.
The field test results in table 3 show that the mixed agent of the penthiopyrad and the pyraclostrobin has good control effect on the gray mold of strawberries compared with the single agent of the penthiopyrad and the pyraclostrobin by taking clear water as a control, and the control effect on the gray mold of strawberries is optimal when the mass ratio of the penthiopyrad to the pyraclostrobin is 1:1.5. The compound preparation not only reduces the dosage and the cost of the dosage, but also is safe for the growth of strawberries.
Field efficacy example 2, strawberry powdery mildew field efficacy example
Test agent: selecting a mixed agent of penthiopyrad and bupirimate prepared in preparation examples 6-10;
20% penthiopyrad suspension (AGRO, sanwell chemical Co., ltd.);
25% bupirimate microemulsion (Simarouble technology Co., ltd.).
Experiments were performed on strawberry bases in Qingdao area Zhao Tunzhen in Shanghai city to verify the control effect of each blended drug on strawberry powdery mildew. The test crop is strawberry, and the variety is Xintun No. one. The test method refers to GB/T17980.119-2004 pesticide field efficacy test criterion (one) efficacy test of the bactericide for preventing and treating powdery mildew of strawberries. The test agents and their dosages are shown in Table 4. A blank control was additionally placed, with each treatment being repeated 4 times, with 20 strawberries per cell, 48 cells total, and a random block arrangement. And uniformly spraying the whole plant by adopting a conventional spraying method. The 1 st application is carried out at the initial stage of strawberry powdery mildew, and the second application is carried out after 14 days, and the total application is carried out for 2 times.
The investigation and statistical method comprises the following steps:
the disease index was investigated 14 days after the first and 14 days after the second, 5 spots were randomly investigated for each cell, all leaves of 3 plants were investigated for each spot, each leaf was recorded in stages according to the percentage of the leaf area of the lesion, the total leaf number of the investigation and the leaf number of each stage were recorded, and the disease index was calculated, and the results are shown in table 4.
The grading criteria are:
level 0: no disease;
stage 1: the connecting area of the disease spots accounts for less than 5% of the whole fruit area;
3 stages: the connecting area of the disease spots accounts for 5-10% of the whole fruit area;
5 stages: the joint area of the disease spots accounts for 11% -25% of the whole fruit area;
7 stages: the joint area of the disease spots accounts for 26-50% of the whole fruit area;
stage 9: the area of the connected disease spots accounts for more than 50% of the whole fruit area.
The disease index and the prevention and treatment effect calculating method comprises the following steps:
safety investigation and impact on other organisms: the effect of each treatment of the agent on strawberry growth and on other diseases was observed on the first day and several days after each spray.
Table 4 results of field efficacy experiments of penthiopyrad and bupirimate mixture for controlling powdery mildew of strawberry
Safety investigation of strawberries shows that each treatment agent does not cause phytotoxicity to strawberry stems and leaves during the test period.
The field test results in table 4 show that, compared with single doses of penthiopyrad and bupirimate, the penthiopyrad and bupirimate have good control effects on strawberry powdery mildew, and the control effect on strawberry powdery mildew is optimal when the mass ratio of the penthiopyrad to the bupirimate is 1:1. The compound preparation not only reduces the dosage and the cost of the dosage, but also is safe for the growth of strawberries.
Example of field efficacy 3, example of field efficacy of melon powdery mildew
Test agent: selecting a mixed agent of penthiopyrad and bupirimate prepared in preparation examples 6-10;
20% penthiopyrad suspension (AGRO, sanwell chemical Co., ltd.);
25% bupirimate microemulsion (Simarouble technology Co., ltd.).
The test was carried out in facility greenhouses of Zhuang Zhenguo villages of Qingjingsu county, qingjingshi, tangshan, hebei province, and the cultivation conditions in the communities were uniform, and melon and nectarine were planted in the surrounding fields. So as to verify the control effect of each mixed pesticide on melon powdery mildew. The test crop is melon, and the variety is Jindian. The test agent and the dosage are shown in Table 5, and the test agent and the control agentCell processing with blank control adopts random block arrangement, and cell area: 32m 2 . Repeated 3 times.
The medicine is applied for 2 times, and the interval period is about 7 days, and the front and back surfaces of stems and leaves are uniformly sprayed. Disease number was investigated prior to administration, mid-term investigation was performed prior to the second administration, and last investigation was performed 7 days after the second administration. Four points are randomly taken from each cell, all leaves of 2 plants are investigated at each point, each leaf is classified according to the percentage of the area of the disease spots to the area of the whole leaf, the total leaf number and the leaf number of each stage of disease are recorded and investigated, and the disease index is calculated, and the result is shown in Table 5.
The grading method comprises the following steps:
level 0: no disease spots
Stage 1: the area of the disease spots accounts for less than 5% of the whole leaf area;
3 stages: the area of the lesion accounts for 6% -10% of the whole leaf area;
5 stages: the area of the lesion accounts for 11% -20% of the whole leaf area;
7 stages: the area of the lesion accounts for 21% -40% of the whole leaf area;
stage 9; the area of the disease spots accounts for more than 40% of the whole leaf area.
The drug effect calculation method comprises the following steps:
safety investigation and impact on other organisms: the first day and several days after each spraying, the effect of each treatment with the agent on melon growth and on other diseases was observed.
Table 5 results of experiments on efficacy of penthiopyrad and bupirimate mixture in fields for controlling powdery mildew of melon
Safety investigation of muskmelon shows that each treatment agent does not generate phytotoxicity on stems and leaves of muskmelon during the test period.
The field test results in Table 5 show that, compared with single doses of penthiopyrad and bupirimate, the penthiopyrad and bupirimate have good control effects on melon powdery mildew, and the control effect on melon powdery mildew is optimal when the mass ratio of the penthiopyrad to the bupirimate is 1:1. The compound preparation not only reduces the dosage and the cost of the dosage, but also is safe for melon growth.
Example of field efficacy 4, example of field efficacy of Botrytis cinerea
Test agent: selecting a mixed agent of penthiopyrad and pyraclostrobin prepared in preparation examples 1-5;
20% penthiopyrad suspension (AGRO, sanwell chemical Co., ltd.);
25% pyraclostrobin suspension (Shandong Kang Qiao Biotechnology Co., ltd.);
the experiment was carried out in the Wang Gang street Zhangdangbao village of Shenyang city of Shenjia in Liaoning province, which has a history of tomato planting for many years, and tomato gray mold occurs throughout the year. To verify the control effect of each mixed pesticide on the gray mold of tomatoes. The test crop is tomato and the variety is gardening winter treasure. The test agent and the dosage are shown in Table 6, the cell treatment of the test agent, the control agent and the blank control adopts random block arrangement, and the cell area is 20m 2 The disease number was investigated before 3 replicates, the total number of administrations was investigated twice, and the control effect was investigated before administration, 7 days after the first administration and 10 days after the second administration. Five-point investigation is randomly carried out in each cell, 2-3 plants are investigated in each spot, and all leaves are investigated in each plant. The disease index was calculated and the results are shown in Table 6.
Tomato fruit pest grading method (in fruit unit):
level 0: no disease spots;
stage 1: onset of residual flowers;
3 stages: disease of the umbilicus;
5 stages: the disease spot length is less than 10 percent of the fruits;
7 stages: the disease spot length accounts for 11-25% of the fruit;
stage 9: the length of the disease spots is more than 26 percent.
The calculation method of the drug effect is that
Safety investigation and impact on other organisms: after the application, the influence of each treatment on crops is observed in detail before the next application, whether symptoms of phytotoxicity (death, growth inhibition, chlorosis, deformity, leaf blight and the like) appear on the tomato plants in the treatment area or not is accurately described, and the record is carried out.
Table 6 results of field efficacy experiments for controlling tomato gray mold by penthiopyrad and pyraclostrobin mixture
Safety investigation of tomatoes shows that each treatment agent does not cause phytotoxicity to tomato stems and leaves in the test period.
The field test results in table 6 show that the mixed agent of the penthiopyrad and the pyraclostrobin has good control effect on the gray mold of tomatoes compared with the single agent of the penthiopyrad and the pyraclostrobin by taking clear water as a control, and has the best control effect on the gray mold of tomatoes when the mass ratio of the penthiopyrad to the pyraclostrobin is 1:1.5. The combination of the two not only reduces the dosage and the cost of the dosage, but also is safe for the growth of tomatoes.
In conclusion, the sterilizing combination containing penthiopyrad has good control effect on gray mold and powdery mildew of crops, and is safe to target crops. The compound preparation not only improves the control effect, but also reduces the dosage and the cost, and is an ideal medicament for preventing and treating gray mold and powdery mildew. Therefore, the application and popularization of the compound preparation have very important significance to society.
The present application is described in detail above. It will be apparent to those skilled in the art that the present application can be practiced in a wide range of equivalent parameters, concentrations, and conditions without departing from the spirit and scope of the application and without undue experimentation. While the application has been described with respect to specific embodiments, it will be appreciated that the application may be further modified. In general, this application is intended to cover any variations, uses, or adaptations of the application following, in general, the principles of the application and including such departures from the present disclosure as come within known or customary practice within the art to which the application pertains. The application of some of the basic features may be done in accordance with the scope of the attached claims.

Claims (6)

1. A compound agricultural sterilization composition is characterized in that: the composite material consists of an active component A and an active component B;
the active component A is penthiopyrad;
the active component B is bupirimate;
the mass ratio of the active component A to the active component B is 1:1.
2. the use of the compound agricultural bactericidal composition of claim 1 for preventing and treating powdery mildew of crops.
3. The compound agricultural bactericide is prepared from the compound agricultural bactericide composition and auxiliary materials acceptable in pesticide preparations, wherein the mass percentage of the compound agricultural bactericide composition is 1-90%.
4. A formulated agricultural fungicide according to claim 3, characterized in that: the formulation of the compound agricultural bactericide is any one of suspending agent, aqueous emulsion, dispersible oil suspending agent, wettable powder, water dispersible granule, emulsifiable concentrate and microemulsion.
5. The use of the compound agricultural bactericide according to claim 3 or 4 for controlling powdery mildew of crops.
6. The use according to claim 5, characterized in that: the crop is strawberry, melon or tomato.
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