CN114891651A - Composite nutrient active yeast, preparation method and application thereof - Google Patents
Composite nutrient active yeast, preparation method and application thereof Download PDFInfo
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- A21D2/00—Treatment of flour or dough by adding materials thereto before or during baking
- A21D2/08—Treatment of flour or dough by adding materials thereto before or during baking by adding organic substances
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- A—HUMAN NECESSITIES
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- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
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- A21D8/02—Methods for preparing dough; Treating dough prior to baking
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- A21D8/047—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with yeasts
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Abstract
The invention discloses a composite nutrient active yeast, a preparation method and application thereof. Wherein, the raw materials of the composite nutrient active yeast comprise: fresh yeast, yeast cell walls containing vitamin D2, beta-glucan and auxiliary materials, wherein the content of vitamin D2 in the composite nutrient active yeast is 250-500 mug per 100g of dry yeast. The active yeast product containing vitamin D2 and beta-glucan complex nutrients can be directly used as common yeast, can simply and conveniently supplement vitamin D and improve immunity through daily dietary reinforcement of baked wheaten food, and is suitable for vegetarians to be used as a supplement choice of the vitamin D.
Description
Technical Field
The invention relates to the technical field of yeast related products, in particular to composite nutrient active yeast, a preparation method and application thereof.
Background
Vitamin D is a lipid-soluble steroid derivative essential to human body, mainly including vitamin D2 and D3, which play an important role in maintaining calcium and phosphorus metabolism and normal physiological functions of human body, and deficiency of vitamin D can lead to rickets in children and osteomalacia and osteoporosis in adults. At present, vitamin D deficiency has become a common phenomenon worldwide. The commercially available supplemental vitamin D is usually present in the form of an oral dosage of synthetic vitamin D3. Vitamin D3 is made from sheep wool by several solvent extractions and chemical treatments prior to crystallization and irradiation, and is therefore not suitable for vegetarians. At present, products which can supplement vitamin D and improve immunity and are suitable for vegetarians are expected to be fortified by daily diet.
Vitamin D2 is a natural form of vegetarian vitamin D, and in some plants and yeast exists as ergosterol, a vitamin D precursor, which produces vitamin D2 upon exposure to UV-B ultraviolet light, and is particularly suitable for use by vegetarians to supplement vitamin D. Currently, there are some methods for preparing vitamin D2, but there are still a series of problems, such as: irradiating the instant dried yeast product under a UV-B ultraviolet lamp for a certain time to obtain instant dried yeast containing high content of vitamin D2; however, this method has the following disadvantages: 1) the ultraviolet radiation of the dry yeast can cause the genetic variation of the strain; 2) direct irradiation of active yeast cells by uv light results in a significant reduction in yeast fermentability; 3) after irradiation, the yeast contains high levels of vitamin D, which is not conducive to small amounts of customer-calculated additions. For another example: the yeast cell walls containing high vitamin D2 content can be obtained by treating the yeast cell wall parts with UV light, or treating the yeast with UV light, followed by autolysis or hydrolysis and further separation. The yeast cell wall can be independently used as a fortified food additive; however, the method for producing yeast cell walls containing a high content of vitamin D provided in this proposal does not have the characteristic of high fermentation power of yeast and is not suitable for addition as a daily diet.
Disclosure of Invention
The invention aims to provide a composite nutrient active yeast, a preparation method and application thereof, so as to provide a vitamin D supplement suitable for vegetarians.
In order to achieve the above object, according to one aspect of the present invention, there is provided a complex nutrient active yeast. The raw materials of the composite nutrient active yeast comprise: fresh yeast, yeast cell walls containing vitamin D2, beta-glucan and auxiliary materials, wherein the content of vitamin D2 in the composite nutrient active yeast is 250-500 mug per 100g of dry yeast.
Furthermore, the solid content of fresh yeast in the composite nutrient active yeast is 33-40 wt%, and the weight percentage of beta-glucan is 2-5%.
Further, the auxiliary materials comprise sorbitan monostearate andor vitamin C.
Furthermore, the dosage of the auxiliary materials is 0.8-1.2% relative to the composite nutritional active yeast.
Further, the preparation method comprises the following steps: mixing fresh yeast, yeast cell wall containing vitamin D2, beta-glucan and adjuvants, extruding into fine strip or pellet, and continuously drying with roller drying bed or fluidized bed to make the water content in the final compound nutrient active yeast less than 6%.
Further, the temperature of the continuous drying in a roller drying bed or a fluidized bed is 30-80 ℃.
Further, yeast cell walls containing vitamin D2 were prepared by the following steps: and (3) irradiating the yeast cell wall powder under a UV-B ultraviolet lamp to obtain the yeast cell wall containing vitamin D2.
According to another aspect of the present invention, there is provided a method for preparing a complex nutrient active yeast. The preparation method comprises the following steps: mixing fresh yeast, yeast cell wall containing vitamin D2, beta-glucan and adjuvants, extruding into fine strip or pellet, and continuously drying with roller drying bed or fluidized bed to make water content in final compound nutrient active yeast less than 6%; preferably, the weight content of the vitamin D2 in the composite nutrient active yeast is 250-500 mug/100 g; the solid content of fresh yeast in the composite nutrient active yeast is 33-40 wt%, and the weight percentage of beta-glucan is 2-5%.
Further, the temperature of the continuous drying by a roller drying bed or a fluidized bed is 30-80 ℃; preferably, the yeast cell wall containing vitamin D2 is prepared by the following steps: and (3) irradiating the yeast cell wall powder under a UV-B ultraviolet lamp to obtain the yeast cell wall containing vitamin D2.
According to a further aspect of the invention there is provided the use of a complex nutrient active yeast as described above in baked pasta.
The active yeast product containing vitamin D2 and beta-glucan complex nutrients can be directly used as common yeast, can simply and conveniently supplement vitamin D and improve immunity through daily dietary reinforcement of baked wheaten food, and is suitable for vegetarians to be used as a supplement choice of the vitamin D.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the invention and, together with the description, serve to explain the invention and not to limit the invention. In the drawings:
FIG. 1 shows a process scheme for an IDY form of complex nutrient yeast according to an embodiment of the present invention;
figure 2 shows a process scheme for ADY form complex nutrient yeast according to an embodiment of the invention.
Detailed Description
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present invention will be described in detail below with reference to the embodiments with reference to the attached drawings.
According to an exemplary embodiment of the present invention, a complex nutrient active yeast is provided. The raw materials comprise: fresh yeast, yeast cell walls containing vitamin D2, beta-glucan and auxiliary materials, wherein the content of vitamin D2 in the composite nutrient active yeast is 250-500 mug per 100g of dry yeast.
The yeast beta-glucan has the physiological functions of reducing cholesterol, regulating blood sugar level, promoting the proliferation of probiotics in intestinal tracts, regulating the immunity of organisms, resisting tumors and the like, and the active yeast product contains vitamin D2 and the beta-glucan, can supplement vitamin D and beta-glucan nutrients for human bodies through diet and improve the immunity of the human bodies. The invention adds vitamin D2 generated by ultraviolet irradiation of yeast cell wall into active yeast product, which can not cause variation of strain and keep high fermentation ability of yeast product and is suitable for vegetarian food.
The active yeast product containing vitamin D2 and beta-glucan complex nutrients can be directly used as common yeast, can simply and conveniently supplement vitamin D and improve immunity through daily dietary reinforcement of baked wheaten food, and is suitable for vegetarians to be used as a supplement choice of the vitamin D.
Preferably, the solid content of fresh yeast in the composite nutrient active yeast is 33-40 wt%, and the weight percentage of beta-glucan is 2-5%.
According to a typical embodiment of the invention, the adjuvants comprise sorbitan monostearate andor vitamin C; preferably, the dosage of the auxiliary material relative to the composite nutritional active yeast is 0.8-1.2%.
According to a typical embodiment of the invention, the preparation method comprises the following steps: mixing fresh yeast, yeast cell wall containing vitamin D2, beta-glucan and adjuvants, extruding into fine strip or pellet, and continuously drying with roller drying bed or fluidized bed to make the water content in the final compound nutrient active yeast less than 6%.
Preferably, the temperature of the continuous drying by a roller drying bed or a fluidized bed is 30-80 ℃, the activity of the yeast can be ensured by drying at the temperature, and the yeast is stored for a long time.
According to an exemplary embodiment of the present invention, the yeast cell wall containing vitamin D2 is prepared by the following steps: and (3) irradiating the yeast cell wall powder under a UV-B ultraviolet lamp to obtain the yeast cell wall containing vitamin D2.
According to an exemplary embodiment of the present invention, a method for preparing a complex nutrient active yeast is provided. The preparation method comprises the following steps: mixing fresh yeast, yeast cell wall containing vitamin D2, beta-glucan and adjuvants, extruding into fine strip or pellet, and continuously drying with roller drying bed or fluidized bed to make water content in final compound nutrient active yeast less than 6%; preferably, the content of the vitamin D2 in the composite nutrient active yeast is 250-500 mug per 100g of dry yeast; the solid content of fresh yeast in the composite nutrient active yeast is 33-40 wt%, and the weight percentage of beta-glucan is 2-5%, preferably 2.8%.
According to a typical embodiment of the invention, the composite nutrient active yeast can be directly used as common yeast, and can simply and conveniently supplement vitamin D and improve immunity through daily dietary enrichment of baked wheaten food. Meanwhile, the product is suitable for vegetarians as a supplement choice of vitamin D.
The following examples are provided to further illustrate the advantageous effects of the present invention.
Example 1
Preparation of cell wall powder containing vitamin D2
The yeast cell wall can be obtained by the following process: the dry matter is yeast milk with concentration of 12-15%, autolysis is carried out at 50-60 ℃, or 1 ‰ catalytic hydrolysis is carried out by adding protease, or mechanical crushing is carried out, heavy phase is separated to obtain cell wall, and the product is obtained by concentrating, spraying and drying. The yeast cell wall can be derived from yeast, such as Saccharomyces cerevisiae, but not limited to Saccharomyces cerevisiae.
The cell wall powder containing vitamin D2 is prepared by the following processes: 100g of yeast cell wall powder was evenly spread over 900cm 2 In the square plate, the intensity of the ultraviolet lamp is respectively exposed to 5-8 mW/cm 2 Continuously turning and irradiating under UV-B ultraviolet lamp for 10min, 20min and 30 min. The vitamin D2 contents of 343 μ g/g, 525.3 μ g/g and 767.6 μ g/g can be obtained respectively.
Example 2
Composite nutrient active yeast in the form of instant dry yeast
Referring to fig. 1, a sugar-free or high-sugar yeast (yeast for flour use) is used for three-stage amplification culture to obtain a fermentation liquid with a thallus concentration of 7%, the fermentation liquid is subjected to centrifugal separation and concentration, water with a certain proportion is added for washing to obtain yeast milk with a thallus content of 25%, vacuum filtration is used for concentration to obtain a fresh yeast sample with a solid content of 36%, the fresh yeast sample is mixed with yeast beta-glucan (with an effective content of 70%) and yeast cell walls containing vitamin D2 (with a content of 401.14 μ g of vitamin D2/g cell walls in the embodiment), the preparation process is the same as that in embodiment 1), and auxiliary materials (such as sorbitan monostearate and the like (the auxiliary materials contain vitamin C) are mixed according to a solid content mass ratio of 95: 2.8: 1.2: 1 to obtain a nutrient emulsified yeast mixed sample containing vitamin D2 and beta-glucan; extruding the mixture to obtain yeast strips, further extruding and granulating the yeast strips by controlling the granulation aperture to be 0.55mm, oscillating to obtain yeast granules with the particle size of 0.55-1 mm, transferring the yeast granules to a fluidized drying bed for drying, setting the air temperature to be 60 ℃ and the air volume to be 120m 3 And h, drying for 20min until the water content is lower than 6 wt%, thus obtaining the instant active dry yeast of the composite nutrient active yeast.
Example 3
Active Yeast containing multiple nutrients in the form of granule ADY (Active Dry Yeast)
Referring to fig. 2, a sugar-free or high-sugar yeast is adopted for three-stage amplification culture to obtain a fermentation liquid with a thallus concentration of 7%, the fermentation liquid is subjected to centrifugal separation and concentration, a certain proportion of water is added for washing to obtain yeast milk with a thallus content of 25%, vacuum filtration is adopted for concentration to obtain a fresh yeast sample with a solid content of 36%, the fresh yeast sample, yeast beta-glucan containing vitamin (effective content 70%), yeast cell walls with a D2 content of 401.14 mug/g and auxiliary materials (the auxiliary materials contain vitamin C) such as sorbitan monostearate are mixed according to a solid content mass ratio of 95: 2.8: 1.2: 1 to obtain a nutrient emulsified yeast mixed sample containing vitamin D2 and beta-glucan; extruding the mixture to obtain yeast strips, further extruding and granulating the yeast strips by controlling the granulation aperture to be 1-3 mm, and oscillating to obtain yeast master batches with the particle size of 1-3 mm; transferring the granulated yeast particles to a roller drying bedThe granular yeast is made into pellet, and the pellet is transferred into fluidized drying bed for drying, with air temperature of 30 deg.C and air volume of 100m 3 And/h, drying for 8h until the water content is lower than 6 wt%, and obtaining the active dry yeast of the spherical particle composite nutrient yeast (the dry yeast in the form needs to be activated by warm water at 38 ℃ for a plurality of minutes before use).
Example 4
Rod-shaped ADY form composite nutrient active yeast
Performing three-stage amplification culture by using sugar-free or high-sugar yeast to obtain fermentation liquor with the thallus concentration of 7%, performing centrifugal separation and concentration, adding a certain proportion of water for washing to obtain yeast milk with the thallus content of 25%, performing vacuum filtration and concentration to obtain a fresh yeast sample with the solid content of 36%, mixing the fresh yeast sample with yeast beta-glucan (effective content 70%), yeast cell walls with vitamin D2 content of 401.14 mu g/g and auxiliary materials (the auxiliary materials contain vitamin C) such as sorbitan monostearate and the like according to the solid content mass ratio of 95: 2.8: 1.2: 1 to obtain a nutrient emulsified yeast mixed sample containing vitamin D2 and beta-glucan; extruding the mixture to obtain yeast strips, further extruding and granulating the yeast strips by controlling the granulation aperture to be 1-3 mm, and oscillating to obtain yeast master batches with the particle size of 1-3 mm; transferring the granular yeast particles into a roller drying bed to make the granular yeast into spherical shape, transferring the spherical yeast particles into a fluidized drying bed for drying, setting the air temperature at 60 deg.C and the air quantity at 100m 3 And/h, drying for 6h until the water content is lower than 6 wt%, and obtaining the active dry yeast of the rod-shaped compound nutrient yeast.
Example 5
Essentially the same as in example 2, except that the yeast pellets were transferred to a fluidized drying bed for drying, the air temperature was set at 80 ℃.
Example 6
Essentially the same as in example 2, except that the cell wall contained vitamin D2 in an amount of 250 μ g vitamin D2/g cell wall and the ratio of yeast to yeast cell wall containing vitamin D2, β -glucan and excipients was 93: 5: 1.0: 0.8.
example 7
Essentially the same as example 2, except that the cell wall contained vitamin D2 in an amount of 500 μ g vitamin D2/g cell wall and the ratio of yeast to yeast cell wall containing vitamin D2, yeast cell wall containing vitamin D2, β -glucan and excipients was 95.8: 2: 1.0: 1.2.
the samples from examples 2 to 7 and the commercial dry yeast products were tested for simulated dough fermentability as described in Q/YB.J19.026, Table 1
TABLE 1 results of the measurement of the leavening ability of the sample of example
| Examples | Sugar-free fermentability mLCO 2 /h | ADY Yeast sugar-free fermentability mLCO 2 /h |
| Example 2 | 800 | - |
| Example 3 | - | 700 |
| Example 4 | - | 725 |
| Example 5 | 780 | - |
| Example 6 | 770 | - |
| Example 7 | 780 | - |
| Commercial dry yeast | 820 | - |
Experiments prove that the operation is feasible, the prepared yeast cell wall containing high-content vitamin D2 is compounded with sugar-free yeast, and spherical granular ADY yeast is obtained by drying in a roller and a fluidized bed, the content of the vitamin D2 is 2.5-5 mu g/g, and the application effect of the prepared yeast cell wall is not obviously different from that of a commercially available common instant dried yeast IDY form.
From the above description, it can be seen that the above-described embodiments of the present invention achieve the following technical effects:
the active yeast product contains vitamin D2 and beta-glucan, can supplement vitamin D and beta-glucan nutrients to human bodies through diet, and improves the immunity of the human bodies.
The invention adds vitamin D2 generated by ultraviolet irradiation of yeast cell wall into active yeast product, which can not cause variation of strain and keep high fermentation ability of yeast product and is suitable for vegetarian food.
One form of the active yeast of the present invention is spherical granular ADY active yeast, which is shelf stable.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. The composite nutrient active yeast is characterized by comprising the following raw materials: fresh yeast, yeast cell walls containing vitamin D2, beta-glucan and auxiliary materials, wherein the content of vitamin D2 in the composite nutrient active yeast is 250-500 mug per 100g of dry yeast.
2. The composite nutrient active yeast as claimed in claim 1, wherein the fresh yeast has a solid content of 33-40 wt% and the beta-glucan is 2-5 wt%.
3. The composite nutrient active yeast according to claim 1, wherein the auxiliary material comprises sorbitan monostearate and/or vitamin C.
4. The composite nutrient active yeast according to claim 3, wherein the amount of the auxiliary material is 0.8-1.2% relative to the amount of the composite nutrient active yeast.
5. The composite nutrient active yeast according to claim 1, which is prepared by the following preparation method: mixing the fresh yeast, the yeast cell wall containing vitamin D2, the beta-glucan and the auxiliary materials, extruding into a thin strip shape or a small ball shape, and continuously drying by a roller drying bed or a fluidized bed to ensure that the water content in the final compound nutrient active yeast is less than 6%.
6. The composite nutrient active yeast according to claim 5, wherein the temperature of the continuous drying in the roller drying bed or the fluidized bed is 30 to 80 ℃.
7. The composite nutrient active yeast according to claim 5, wherein the yeast cell wall containing vitamin D2 is prepared by the following steps: and (3) irradiating the yeast cell wall powder under a UV-B ultraviolet lamp to obtain the yeast cell wall containing vitamin D2.
8. A preparation method of composite nutrient active yeast is characterized by comprising the following steps: mixing fresh yeast, yeast cell wall containing vitamin D2, beta-glucan and auxiliary materials, extruding into thin strips or small balls, and continuously drying by a roller drying bed or a fluidized bed to ensure that the water content in the compound nutrient active yeast is less than 6 percent;
preferably, the content of the vitamin D2 in the composite nutrient active yeast is 250-500 mug per 100g of dry yeast; the solid content of the fresh yeast in the composite nutrient active yeast is 33-40 wt%, and the weight percentage of the beta-glucan is 2-5%.
9. The method according to claim 8, wherein the temperature of the drum drying bed or the fluidized bed is 30 to 80 ℃ during continuous drying;
preferably, the yeast cell wall containing vitamin D2 is prepared by the following steps: and (3) irradiating the yeast cell wall powder under a UV-B ultraviolet lamp to obtain the yeast cell wall containing vitamin D2.
10. Use of a complex nutrient active yeast according to any one of claims 1 to 7 in baked pasta.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105861480A (en) * | 2015-01-19 | 2016-08-17 | 安琪酵母股份有限公司 | Yeast enriching vitamin D2 and production technology thereof |
| JP2019068843A (en) * | 2018-12-28 | 2019-05-09 | ダンスター・フェルメント・アーゲー | Yeast cell wall containing vitamin d2, use of the same, and method for producing the same |
| CN111066952A (en) * | 2019-12-26 | 2020-04-28 | 安琪酵母（伊犁）有限公司 | High-temperature-resistant spherical yeast particles, preparation method thereof and complete granular material |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105861480A (en) * | 2015-01-19 | 2016-08-17 | 安琪酵母股份有限公司 | Yeast enriching vitamin D2 and production technology thereof |
| JP2019068843A (en) * | 2018-12-28 | 2019-05-09 | ダンスター・フェルメント・アーゲー | Yeast cell wall containing vitamin d2, use of the same, and method for producing the same |
| CN111066952A (en) * | 2019-12-26 | 2020-04-28 | 安琪酵母（伊犁）有限公司 | High-temperature-resistant spherical yeast particles, preparation method thereof and complete granular material |
Non-Patent Citations (1)
| Title |
|---|
| 刘静;刘聚祥;: "酵母菌的营养特性及在畜牧业中的应用" * |
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