CN114891551B - 一种生物细胞氢载体骨架的制备方法及其组合物 - Google Patents
一种生物细胞氢载体骨架的制备方法及其组合物 Download PDFInfo
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- CN114891551B CN114891551B CN202210408885.3A CN202210408885A CN114891551B CN 114891551 B CN114891551 B CN 114891551B CN 202210408885 A CN202210408885 A CN 202210408885A CN 114891551 B CN114891551 B CN 114891551B
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- biological cell
- hydrogen carrier
- cell hydrogen
- carrier skeleton
- hydrogen
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Abstract
本发明公开了一种生物细胞氢载体骨架的制备方法及其组合物，方法包括如下步骤：原料处理；培育生长。组合物包括如下组分：聚山梨醇脂、脂醚、脂肪醇环氧乙烷、生物细胞氢载体骨架；脂醚、椰油酰胺丙基甜菜碱、脂肪醇环氧乙、生物细胞氢载体骨架；十二月桂酸脂、醇醚、脂肪醇环氧乙烷、生物细氢载体骨架；亚油酸甘油脂、醇醚、脂肪醇环氧乙烷、生物细胞氢载体骨架。生物细胞氢载体骨架具有良好的乳化、润湿、扩散、增溶、防腐抗氧化、助清洁内燃系统、降低内燃机因高温氧化、抗静电等作用。生物细胞氢载体骨架组合物与碳氢化合物无限溶合，微分子亲合在电子核上，燃烧过程中产生了裂变助燃现象，提高了热值，燃烧更彻底更充分使动力增强。
Description
技术领域
本发明属于生物细胞化学工程领域，涉及一种生物细胞氢载体骨架的制备方法及其组合物。
背景技术
进入二十一世纪，氢能源作为绿色能源具有可持续发展的趋势，从1766年英国科学家卡文迪向英国皇家学会提交的《人造空气实验》研究报告以来，引起了无数科学家对氢能的研究，例如：煤炭制氢、石油制氢、天然气制氢、甲烷制氢、电解水制氢及氢能电池等。上世纪美、日、意科学家曾在化工等领域利用生物制取生物催化剂，但因成本过高难以继续发展，多次以生物为原料制取氢燃料也因成本过高放弃产出。
在我国水葫芦于20世纪30年代作为花卉引入，后来又作为猪饲料在大江湖泊蔓延，广泛分布于大江南北，如华北、华东、华中、华南等十几个省市，对农牧业等造成严重危害。在2014年举行的中国外来物种入侵生物水葫芦现场灭除活动中获悉，2004年—2014年十年间，新增入侵物种近50种，20种在中国国土上呈现出更快的增长，大面积成灾。同时，由于海洋污染日益加重，海澡泛滥成灾。如何利用莲藕径叶、水葫芦、海藻、油藻等水生植物，研发生物细胞氢载体骨架，使其变废为宝，避免环境危害，是一种行之有效的解决方法。
发明内容
本发明的目的是提供一种能够使水生植物变废为宝，避免环境危害的生物细胞氢载体骨架制备方法及其组合物。
本发明采用的技术方案为：
一种生物细胞氢载体骨架的制备方法，包括如下步骤：
A原料处理：
①将水生植物清洗，在1500r/min-3000r/min转速下粉碎成浆，经过60目-120目过滤滤出粗渣；②将滤得浆液通入紫外线玻璃管道中，在80W—100W功率下，杀菌10min-15min，杀掉杂菌，即得水生植物浆液，滤液收率为40％-60％；
B培育生长：
①按照水生植物浆液10％-20％、无菌小分子水60％-90％、载体蛋白酶1％-5％、连体蛋白1％-5％、受体蛋白1％-5％的质量百份比称取原料，上述原料的质量百份之和为100％；②将上述原料分别投入不绣钢保温容器内，该不绣钢保温容器分别设有上进料口、下出料口及带阀门的中取样口；③原料投入后，关闭进料口，开始缓慢升温至40℃，④通入氢气，通氢压力控制在0.001MPa-0.015MPa持续180min-500min，⑤控制温度25℃-40℃保持180min-200min后，通过中取样口取样，停止加热及通入氢气，最后通过下出料口将生物细胞氢载体骨架抽出，即得。
上述步骤A中水生植物包括莲藕径叶、水葫芦、海藻、油藻。
一种生物细胞氢载体骨架组合物，包括如下组分及质量百分比：
一、聚山梨醇脂2％-5％、脂醚1％-5％、脂肪醇环氧乙烷3％-5％、生物细胞氢载体骨架余量。
二、脂醚1％-5％、椰油酰胺丙基甜菜碱0.5％-3％、脂肪醇环氧乙烷2％-5％、生物细胞氢载体骨架余量。
三、十二月桂酸脂3％-5％、醇醚0.5％-3％、脂肪醇环氧乙烷2％-5％、生物细氢载体骨架余量。
四、亚油酸甘油脂3％-6％、醇醚0.6％-2.5％、脂肪醇环氧乙烷2％-5％、生物细胞氢载体骨架余量。
生物细胞氢载体骨架是无毒透明水状液体，略苦，密度0.9g/cm3—1.0g/cm3，在-3℃-5℃下结冰。其具有良好的乳化、润湿、扩散、增溶、防腐抗氧化、助清洁内燃系统、降低内燃机因高温氧化、抗静电等作用。
生物细胞氢载体骨架组合物与碳氢化合物碳水化合物溶合(醇类、油类苯类无限溶合)无腐蚀，pH值为5.5-8.5之间，在与高能燃料碳氢化合物、碳水化合混合下，不燃烧不爆炸，常温封闭下不变质耐储存。其生产过程中所产生的副产品——滤渣，可以制造沼气、绿色有机肥等，每吨滤渣可产沼气200m3-246m3，绿色有机肥0.45吨-0.6吨。
生物细胞氢载体骨架组合物的作用原理如下：
1、生物细胞氢载体骨架组合物溶入燃油中，形成氢网络骨架稳固溶合在碳氢化合健上，燃油燃烧过程中氢产生裂变助燃现象，使燃油燃烧更重分，燃烧室内温度迅速升高，动力更强进排放降低是在燃油动能中节能环保途径。2、生物细胞氢载体骨架组合物是一种生物细胞基因组织，其由载体蛋白酶、连体蛋白、受体蛋白酶和活化氢存在下，培养而形成的微生物网络骨架载体。3、生物细胞氢载体骨架组合物加入燃油中，与燃油充分分散，溶合、亲合在碳分子电子核上，结合数个氢分子团，在内机燃烧过程中与碳氢化合物共燃，产生裂变聚变现象，使碳氢化合物燃烧充分，化合物排出排气管道外。由于燃烧值提高，动力增强，减少了油耗，燃烧重分，燃机内积炭减少。4、生物细胞氢载体骨架组合物含有一定量表面活性基因，具有清洗内燃系统作用，由于机体表面积炭及胶质减少，乃导致润滑油受热均衡，延迟老化、氧化、硫化等现象，延长了润滑油使用寿命，更换次数降低。
生物细胞氢载体骨架组合物具有如下优点：
1、生物细胞氢载体骨架组合物包括：阴阳离子表面活性剂、脂醚、椰油酰胺、丙基甜菜碱、十二月桂酸脂、亚油酸甘油脂、脂肪醇环氧乙琓、醇醚等，它们是具有良好乳化、润湿、扩散、增溶、防腐抗氧化、助清洁清除污垢内燃机燃烧系统、降低内燃机因高温氧化修复、抗静电等性能。
2、生物细胞氢载体骨架组合物与碳氢化合物无限溶合，微分子亲合在电子核上，燃烧过程中产生了裂变助燃现象，提高了热值，燃烧更彻底更充分使动力增强。
3、生物细胞氢载体骨架组合物与碳氢化合物混合燃烧过程中，由于增加了N个氢分子，氢健所燃烧后混合气体大部分变化为碳水化合物排出。
4、生物细胞氢载体骨架组合物在E92#、E95#、E98#、92#、95#、98#车用汽油，按0.1％-0.3％比例加入时，节油10％-20％，降低排放有害物排放20％-30％。生物细胞氢载体骨架组合物在润滑油S型系列、Y型系列、Q型系列、W型系列中加入时，分别多行驶2500km-3000km。生物细胞氢载体骨架组合物在柴油5#、0#、-10#、-20#、-30#、-35#，其中包括(国五，国六)型，按照0.1％-0.5％比例加入时，可提高行驶里程10％-20％。生物细胞氢载体骨架组合物在船燃4#、5#、180#、240#，分别按照3％-5％加入时，可节油12％-16％，减少烟尘30％、硫化物15％、氮化物5％，一氧化碳总排放量下降18％-25％。
5、生物细胞氢载体骨架组合物的功能是与碳氢化合物充分溶合，微分子亲合在电子核上，燃烧过程中产生了裂变巨变现象提高了热值，燃烧更彻底、更充分，使动力增强。增加了多个氢分子，氢健燃烧后混合气体大部分变化为碳水化合物排出。添加了阴阳离子活性剂等，能有效洁清内燃机系统积炭污垢，从而减少了棵粒物及有害气体排放，防止了润滑油老化延长了润滑油使用寿命，减少润滑油更换濒率。
6、生物细胞氢载体骨架组合物制取过程中对环境不造成污染，原料易得供应充足，生产成本低廉，终端零售价格不超过10元，远低于市场同类产品，而且还是具多功能节油，清洁内燃机系统的作用。
具体实施方式
实施例一：一种生物细胞氢载体骨架制备方法，包括如下步骤：
A、原料处理：
①将水生植物清洗，在1500r/min-3000r/min转速下粉碎成浆，经60目-120目过滤滤出粗渣；②将滤得浆液通入紫外线玻璃管道中，在80W—100W功率下，杀菌10min-15min，杀掉杂菌，即得水生植物浆液，滤液收率为40％-60％；
B、培育生长：
①按照水生植物浆液10％-20％、无菌小分子水60％-90％、载体蛋白酶1％-5％、连体蛋白1％-5％、受体蛋白1％-5％的质量百份比称取原料，上述原料的质量百份之和为100％；②将上述原料分别投入0.02m3不绣钢保温容器内，该不绣钢保温容器分别设有上进料口、下出料口及带阀门的中取样口；③原料投入后，关闭进料口，开始缓慢升温至40℃，④通入氢气，通氢压力控制在0.001MPa-0.015MPa持续180min-300min，⑤控制温度25℃-40℃保持180min-200min后，通过中取样口取样，停止加热及通入氢气，最后通过下出料口将生物细胞氢载体骨架抽出，即得。
实施例二：一种生物细胞氢载体骨架制备方法，包括如下步骤：
A、原料处理：
①将水生植物清洗，在1500r/min-3000r/min转速下粉碎成浆，经60目-120目过滤滤出粗渣；②将滤得浆液通入紫外线玻璃管道中，在80W—100W功率下，杀菌10min-15min，杀掉杂菌，即得水生植物浆液，滤液收率为40％-60％；
B、培育生长：
①按照水生植物浆液10％-20％、无菌小分子水65％-80％、载体蛋白酶1％-5％、连体蛋白1％-5％、受体蛋白1％-5％的质量百份比称取原料，上述原料的质量百份之和为100％；②将上述原料分别投入0.02m3不绣钢保温容器内，该不绣钢保温容器分别设有上进料口、下出料口及带阀门的中取样口；③原料投入后，关闭进料口，开始缓慢升温至40℃，④通入氢气，通氢压力控制在0.001MPa-0.015MPa持续300min-400min，⑤控制温度25℃-40℃保持180min-200min后，通过中取样口取样，停止加热及通入氢气，最后通过下出料口将生物细胞氢载体骨架抽出，即得。
实施例三：一种生物细胞氢载体骨架制备方法，包括如下步骤：
A、原料处理：
①将水生植物清洗，在1500r/min-3000r/min转速下粉碎成浆，经60目-120目过滤滤出粗渣；②将滤得浆液通入紫外线玻璃管道中，在80W—100W功率下，杀菌10min-15min，杀掉杂菌，即得水生植物浆液，滤液收率为40％-60％；
B、培育生长：
①按照水生植物浆液10％-20％、无菌小分子水70％-90％、载体蛋白酶1％-5％、连体蛋白1％-5％、受体蛋白1％-5％的质量百份比称取原料，上述原料的质量百份之和为100％；②将上述原料分别投入0.02m3不绣钢保温容器内，该不绣钢保温容器分别设有上进料口、下出料口及带阀门的中取样口；③原料投入后，关闭进料口，开始缓慢升温至40℃，④通入氢气，通氢压力控制在0.001MPa-0.015MPa持续400min-500min，⑤控制温度25℃-40℃保持180min-200min后，通过中取样口取样，停止加热及通入氢气，最后通过下出料口将生物细胞氢载体骨架抽出，即得。
实施例四：一种生物细胞氢载体骨架组合物，包括如下组分及质量百分比：
聚山梨醇脂2％、脂醚1％、脂肪醇环氧乙烷3％、生物细胞氢载体骨架余量。
实施例五：一种生物细胞氢载体骨架组合物，包括如下组分及质量百分比：
聚山梨醇脂5％、脂醚5％、脂肪醇环氧乙烷5％、生物细胞氢载体骨架余量。
实施例六：一种生物细胞氢载体骨架组合物，包括如下组分及质量百分比：
脂醚1％、椰油酰胺丙基甜菜碱0.5％、脂肪醇环氧乙烷2％、生物细胞氢载体骨架余量。
实施例七：一种生物细胞氢载体骨架组合物，包括如下组分及质量百分比：
脂醚5％、椰油酰胺丙基甜菜碱3％、脂肪醇环氧乙烷5％、生物细胞氢载体骨架余量。
实施例八：一种生物细胞氢载体骨架组合物，包括如下组分及质量百分比：
十二月桂酸脂3％、醇醚0.5％、脂肪醇环氧乙烷2％、生物细氢载体骨架余量。
实施例九：一种生物细胞氢载体骨架组合物，包括如下组分及质量百分比：
十二月桂酸脂5％、醇醚3％、脂肪醇环氧乙烷5％、生物细氢载体骨架余量。
实施例十：一种生物细胞氢载体骨架组合物，包括如下组分及质量百分比：
亚油酸甘油脂3％、醇醚0.6％％、脂肪醇环氧乙烷2％、生物细胞氢载体骨架余量。
实施例十一：一种生物细胞氢载体骨架组合物，包括如下组分及质量百分比：
亚油酸甘油脂6％、醇醚2.5％、脂肪醇环氧乙烷5％、生物细胞氢载体骨架余量。
本发明生物细胞氢载体骨架组合物经实验所得数据及对比数据见下列各表：
表1、生物细胞氢载体骨架组合物与无添加国家标准排气放对比：
无添加国五国六排放标准
加入生物细胞氢载体骨架组合物后实际测试指标
表2、生物细胞氢载体骨架组合物加入1#汽油中实验平台三菱四缸100马力功率74KW排气量1584cc
表3、生物细胞氢载体骨架组合物加入1#汽油0.1％中，实验平台六缸3.5通用发动机
表4、平台试验三菱四缸100马力功率74KW排气量1584cc
表5、路面试验三菱四缸100马力功率74kw排气量1584cc装车实验承载1000kg
表6、发动机型号路面试验无锡柴油6113350马力257kw6轴标载总重49t国五0#柴油中：
表7、企业标准——生物细胞氢载体骨架组合物
表8、企业标准——生物细胞氢载体骨架组合物
Claims (2)
1.一种生物细胞氢载体骨架的制备方法，包括如下步骤：
A原料处理：
①将水生植物清洗，在1500r/min-3000r/min转速下粉碎成浆，经过60目-120目过滤滤出粗渣；②将滤得浆液通入紫外线玻璃管道中，在80W—100W功率下，杀菌10min-15min，杀掉杂菌，即得水生植物浆液，滤液收率为40％-60％；
B培育生长：
①按照水生植物浆液10％-20％、无菌小分子水60％-90％、载体蛋白酶1％-5％、连体蛋白1％-5％、受体蛋白1％-5％的质量百份比称取原料，上述原料的质量百份之和为100％；②将上述原料分别投入不绣钢保温容器内，该不绣钢保温容器分别设有上进料口、下出料口及带阀门的中取样口；③原料投入后，关闭进料口，开始缓慢升温至40℃，④通入氢气，通氢压力控制在0.001MPa-0.015MPa持续180min-500min，⑤控制温度25℃-40℃保持180min-200min后，通过中取样口取样，停止加热及通入氢气，最后通过下出料口将生物细胞氢载体骨架抽出，即得。
2.根据权利要求1所述的一种生物细胞氢载体骨架的制备方法，其特征是：所述步骤A中水生植物选自莲藕径叶、水葫芦、海藻、油藻中的一种或多种。
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