CN114891551A - 一种生物细胞氢载体骨架的制备方法及其组合物 - Google Patents

一种生物细胞氢载体骨架的制备方法及其组合物 Download PDF

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CN114891551A
CN114891551A CN202210408885.3A CN202210408885A CN114891551A CN 114891551 A CN114891551 A CN 114891551A CN 202210408885 A CN202210408885 A CN 202210408885A CN 114891551 A CN114891551 A CN 114891551A
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谭永君
谭清峰
陈昕昕
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FUSHUN WANGHUA YANWU CHEMICAL PLANT
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Abstract

本发明公开了一种生物细胞氢载体骨架的制备方法及其组合物，方法包括如下步骤：原料处理；培育生长。组合物包括如下组分：聚山梨醇脂、脂醚、脂肪醇环氧乙烷、生物细胞氢载体骨架；脂醚、椰油酰胺丙基甜菜碱、脂肪醇环氧乙、生物细胞氢载体骨架；十二月桂酸脂、醇醚、脂肪醇环氧乙烷、生物细氢载体骨架；亚油酸甘油脂、醇醚、脂肪醇环氧乙烷、生物细胞氢载体骨架。生物细胞氢载体骨架具有良好的乳化、润湿、扩散、增溶、防腐抗氧化、助清洁内燃系统、降低内燃机因高温氧化、抗静电等作用。生物细胞氢载体骨架组合物与碳氢化合物无限溶合，微分子亲合在电子核上，燃烧过程中产生了裂变助燃现象，提高了热值，燃烧更彻底更充分使动力增强。

Description

一种生物细胞氢载体骨架的制备方法及其组合物
技术领域
本发明属于生物细胞化学工程领域，涉及一种生物细胞氢载体骨架的制备 方法及其组合物。
背景技术
进入二十一世纪，氢能源作为绿色能源具有可持续发展的趋势，从1766年 英国科学家卡文迪向英国皇家学会提交的《人造空气实验》研究报告以来，引 起了无数科学家对氢能的研究，例如：煤炭制氢、石油制氢、天然气制氢、甲 烷制氢、电解水制氢及氢能电池等。上世纪美、日、意科学家曾在化工等领域 利用生物制取生物催化剂，但因成本过高难以继续发展，多次以生物为原料制 取氢燃料也因成本过高放弃产出。
在我国水葫芦于20世纪30年代作为花卉引入，后来又作为猪饲料在大江 湖泊蔓延，广泛分布于大江南北，如华北、华东、华中、华南等十几个省市， 对农牧业等造成严重危害。在2014年举行的中国外来物种入侵生物水葫芦现场 灭除活动中获悉，2004年—2014年十年间，新增入侵物种近50种，20种在中 国国土上呈现出更快的增长，大面积成灾。同时，由于海洋污染日益加重，海 澡泛滥成灾。如何利用莲藕径叶、水葫芦、海藻、油藻等水生植物，研发生物 细胞氢载体骨架，使其变废为宝，避免环境危害，是一种行之有效的解决方法。
发明内容
本发明的目的是提供一种能够使水生植物变废为宝，避免环境危害的生物 细胞氢载体骨架制备方法及其组合物。
本发明采用的技术方案为：
一种生物细胞氢载体骨架的制备方法，包括如下步骤：
A原料处理：
①将水生植物清洗，在1500r/min-3000r/min转速下粉碎成浆，经过60目-120 目过滤滤出粗渣；②将滤得浆液通入紫外线玻璃管道中，在80W—100W功率下， 杀菌10min-15min，杀掉杂菌，即得水生植物浆液，滤液收率为40％-60％；
B培育生长：
①按照水生植物浆液10％-20％、无菌小分子水60％-90％、载体蛋白酶 1％-5％、连体蛋白1％-5％、受体蛋白1％-5％的质量百份比称取原料，上述原料 的质量百份之和为100％；②将上述原料分别投入不绣钢保温容器内，该不绣钢 保温容器分别设有上进料口、下出料口及带阀门的中取样口；③原料投入后， 关闭进料口，开始缓慢升温至40℃，④通入氢气，通氢压力控制在 0.001MPa-0.015MPa持续180min-500min，⑤控制温度25℃-40℃保持 180min-200min后，通过中取样口取样，停止加热及通入氢气，最后通过下出料 口将生物细胞氢载体骨架抽出，即得。
上述步骤A中水生植物包括莲藕径叶、水葫芦、海藻、油藻。
一种生物细胞氢载体骨架组合物，包括如下组分及质量百分比：
一、聚山梨醇脂2％-5％、脂醚1％-5％、脂肪醇环氧乙烷3％-5％、生物细胞 氢载体骨架余量。
二、脂醚1％-5％、椰油酰胺丙基甜菜碱0.5％-3％、脂肪醇环氧乙烷2％-5％、 生物细胞氢载体骨架余量。
三、十二月桂酸脂3％-5％、醇醚0.5％-3％、脂肪醇环氧乙烷2％-5％、生物 细氢载体骨架余量。
四、亚油酸甘油脂3％-6％、醇醚0.6％-2.5％、脂肪醇环氧乙烷2％-5％、生 物细胞氢载体骨架余量。
生物细胞氢载体骨架是无毒透明水状液体，略苦，密度0.9g/cm3—1.0g/cm 3，在-3℃-5℃下结冰。其具有良好的乳化、润湿、扩散、增溶、防腐抗氧化、 助清洁内燃系统、降低内燃机因高温氧化、抗静电等作用。
生物细胞氢载体骨架组合物与碳氢化合物碳水化合物溶合(醇类、油类苯 类无限溶合)无腐蚀，pH值为5.5-8.5之间，在与高能燃料碳氢化合物、碳水化 合混合下，不燃烧不爆炸，常温封闭下不变质耐储存。其生产过程中所产生的 副产品——滤渣，可以制造沼气、绿色有机肥等，每吨滤渣可产沼气200m3-246m 3，绿色有机肥0.45吨-0.6吨。
生物细胞氢载体骨架组合物的作用原理如下：
1、生物细胞氢载体骨架组合物溶入燃油中，形成氢网络骨架稳固溶合在碳 氢化合健上，燃油燃烧过程中氢产生裂变助燃现象，使燃油燃烧更重分，燃烧 室内温度迅速升高，动力更强进排放降低是在燃油动能中节能环保途径。2、生 物细胞氢载体骨架组合物是一种生物细胞基因组织，其由载体蛋白酶、连体蛋 白、受体蛋白酶和活化氢存在下，培养而形成的微生物网络骨架载体。3、生物 细胞氢载体骨架组合物加入燃油中，与燃油充分分散，溶合、亲合在碳分子电 子核上，结合数个氢分子团，在内机燃烧过程中与碳氢化合物共燃，产生裂变 聚变现象，使碳氢化合物燃烧充分，化合物排出排气管道外。由于燃烧值提高， 动力增强，减少了油耗，燃烧重分，燃机内积炭减少。4、生物细胞氢载体骨架 组合物含有一定量表面活性基因，具有清洗内燃系统作用，由于机体表面积炭 及胶质减少，乃导致润滑油受热均衡，延迟老化、氧化、硫化等现象，延长了 润滑油使用寿命，更换次数降低。
生物细胞氢载体骨架组合物具有如下优点：
1、生物细胞氢载体骨架组合物包括：阴阳离子表面活性剂、脂醚、椰油酰 胺、丙基甜菜碱、十二月桂酸脂、亚油酸甘油脂、脂肪醇环氧乙琓、醇醚等， 它们是具有良好乳化、润湿、扩散、增溶、防腐抗氧化、助清洁清除污垢内燃 机燃烧系统、降低内燃机因高温氧化修复、抗静电等性能。
2、生物细胞氢载体骨架组合物与碳氢化合物无限溶合，微分子亲合在电子 核上，燃烧过程中产生了裂变助燃现象，提高了热值，燃烧更彻底更充分使动 力增强。
3、生物细胞氢载体骨架组合物与碳氢化合物混合燃烧过程中，由于增加了 N个氢分子，氢健所燃烧后混合气体大部分变化为碳水化合物排出。
4、生物细胞氢载体骨架组合物在E92#、E95#、E98#、92#、95#、98#车用 汽油，按0.1％-0.3％比例加入时，节油10％-20％，降低排放有害物排放20％-30％。 生物细胞氢载体骨架组合物在润滑油S型系列、Y型系列、Q型系列、W型系 列中加入时，分别多行驶2500km-3000km。生物细胞氢载体骨架组合物在柴油 5#、0#、-10#、-20#、-30#、-35#，其中包括(国五，国六)型，按照0.1％-0.5％ 比例加入时，可提高行驶里程10％-20％。生物细胞氢载体骨架组合物在船燃4#、 5#、180#、240#，分别按照3％-5％加入时，可节油12％-16％，减少烟尘30％、 硫化物15％、氮化物5％，一氧化碳总排放量下降18％-25％。
5、生物细胞氢载体骨架组合物的功能是与碳氢化合物充分溶合，微分子亲 合在电子核上，燃烧过程中产生了裂变巨变现象提高了热值，燃烧更彻底、更 充分，使动力增强。增加了多个氢分子，氢健燃烧后混合气体大部分变化为碳 水化合物排出。添加了阴阳离子活性剂等，能有效洁清内燃机系统积炭污垢， 从而减少了棵粒物及有害气体排放，防止了润滑油老化延长了润滑油使用寿命， 减少润滑油更换濒率。
6、生物细胞氢载体骨架组合物制取过程中对环境不造成污染，原料易得供 应充足，生产成本低廉，终端零售价格不超过10元，远低于市场同类产品，而 且还是具多功能节油，清洁内燃机系统的作用。
具体实施方式
实施例一：一种生物细胞氢载体骨架制备方法，包括如下步骤：
A、原料处理：
①将水生植物清洗，在1500r/min-3000r/min转速下粉碎成浆，经60目-120 目过滤滤出粗渣；②将滤得浆液通入紫外线玻璃管道中，在80W—100W功率下， 杀菌10min-15min，杀掉杂菌，即得水生植物浆液，滤液收率为40％-60％；
B、培育生长：
①按照水生植物浆液10％-20％、无菌小分子水60％-90％、载体蛋白酶1％-5％、连体蛋白1％-5％、受体蛋白1％-5％的质量百份比称取原料，上述原料的质量百份 之和为100％；②将上述原料分别投入0.02m3不绣钢保温容器内，该不绣钢保温 容器分别设有上进料口、下出料口及带阀门的中取样口；③原料投入后，关闭 进料口，开始缓慢升温至40℃，④通入氢气，通氢压力控制在0.001MPa-0.015MPa 持续180min-300min，⑤控制温度25℃-40℃保持180min-200min后，通过中取 样口取样，停止加热及通入氢气，最后通过下出料口将生物细胞氢载体骨架抽 出，即得。
实施例二：一种生物细胞氢载体骨架制备方法，包括如下步骤：
A、原料处理：
①将水生植物清洗，在1500r/min-3000r/min转速下粉碎成浆，经60目-120 目过滤滤出粗渣；②将滤得浆液通入紫外线玻璃管道中，在80W—100W功率下， 杀菌10min-15min，杀掉杂菌，即得水生植物浆液，滤液收率为40％-60％；
B、培育生长：
①按照水生植物浆液10％-20％、无菌小分子水65％-80％、载体蛋白酶1％-5％、连体蛋白1％-5％、受体蛋白1％-5％的质量百份比称取原料，上述原料的质量百份 之和为100％；②将上述原料分别投入0.02m3不绣钢保温容器内，该不绣钢保温 容器分别设有上进料口、下出料口及带阀门的中取样口；③原料投入后，关闭 进料口，开始缓慢升温至40℃，④通入氢气，通氢压力控制在0.001MPa-0.015MPa 持续300min-400min，⑤控制温度25℃-40℃保持180min-200min后，通过中取 样口取样，停止加热及通入氢气，最后通过下出料口将生物细胞氢载体骨架抽 出，即得。
实施例三：一种生物细胞氢载体骨架制备方法，包括如下步骤：
A、原料处理：
①将水生植物清洗，在1500r/min-3000r/min转速下粉碎成浆，经60目-120 目过滤滤出粗渣；②将滤得浆液通入紫外线玻璃管道中，在80W—100W功率下， 杀菌10min-15min，杀掉杂菌，即得水生植物浆液，滤液收率为40％-60％；
B、培育生长：
①按照水生植物浆液10％-20％、无菌小分子水70％-90％、载体蛋白酶1％-5％、连体蛋白1％-5％、受体蛋白1％-5％的质量百份比称取原料，上述原料的质量百份 之和为100％；②将上述原料分别投入0.02m3不绣钢保温容器内，该不绣钢保温 容器分别设有上进料口、下出料口及带阀门的中取样口；③原料投入后，关闭 进料口，开始缓慢升温至40℃，④通入氢气，通氢压力控制在0.001MPa-0.015MPa 持续400min-500min，⑤控制温度25℃-40℃保持180min-200min后，通过中取 样口取样，停止加热及通入氢气，最后通过下出料口将生物细胞氢载体骨架抽 出，即得。
实施例四：一种生物细胞氢载体骨架组合物，包括如下组分及质量百分比：
聚山梨醇脂2％、脂醚1％、脂肪醇环氧乙烷3％、生物细胞氢载体骨架余量。
实施例五：一种生物细胞氢载体骨架组合物，包括如下组分及质量百分比：
聚山梨醇脂5％、脂醚5％、脂肪醇环氧乙烷5％、生物细胞氢载体骨架余量。
实施例六：一种生物细胞氢载体骨架组合物，包括如下组分及质量百分比：
脂醚1％、椰油酰胺丙基甜菜碱0.5％、脂肪醇环氧乙烷2％、生物细胞氢载 体骨架余量。
实施例七：一种生物细胞氢载体骨架组合物，包括如下组分及质量百分比：
脂醚5％、椰油酰胺丙基甜菜碱3％、脂肪醇环氧乙烷5％、生物细胞氢载体 骨架余量。
实施例八：一种生物细胞氢载体骨架组合物，包括如下组分及质量百分比：
十二月桂酸脂3％、醇醚0.5％、脂肪醇环氧乙烷2％、生物细氢载体骨架余 量。
实施例九：一种生物细胞氢载体骨架组合物，包括如下组分及质量百分比：
十二月桂酸脂5％、醇醚3％、脂肪醇环氧乙烷5％、生物细氢载体骨架余量。
实施例十：一种生物细胞氢载体骨架组合物，包括如下组分及质量百分比：
亚油酸甘油脂3％、醇醚0.6％％、脂肪醇环氧乙烷2％、生物细胞氢载体骨 架余量。
实施例十一：一种生物细胞氢载体骨架组合物，包括如下组分及质量百分 比：
亚油酸甘油脂6％、醇醚2.5％、脂肪醇环氧乙烷5％、生物细胞氢载体骨架 余量。
本发明生物细胞氢载体骨架组合物经实验所得数据及对比数据见下列各 表：
表1、生物细胞氢载体骨架组合物与无添加国家标准排气放对比：
无添加国五国六排放标准
Figure BDA0003603289580000061
Figure BDA0003603289580000071
Figure BDA0003603289580000072
加入生物细胞氢载体骨架组合物后实际测试指标
Figure BDA0003603289580000073
表2、生物细胞氢载体骨架组合物加入1#汽油中实验平台三菱四缸100马 力功率74KW排气量1584cc
Figure BDA0003603289580000074
表3、生物细胞氢载体骨架组合物加入1#汽油0.1％中，实验平台六缸3.5 通用发动机
Figure BDA0003603289580000075
表4、平台试验三菱四缸100马力功率74KW排气量1584cc
Figure BDA0003603289580000081
表5、路面试验三菱四缸100马力功率74kw排气量1584cc装车实验承载 1000kg
Figure BDA0003603289580000082
表6、发动机型号路面试验无锡柴油6113 350马力257kw 6轴标载总重49t 国五0#柴油中：
Figure BDA0003603289580000083
表7、企业标准——生物细胞氢载体骨架组合物
Figure BDA0003603289580000084
表8、企业标准——生物细胞氢载体骨架组合物
Figure BDA0003603289580000085
Figure BDA0003603289580000091

Claims (6)

1.一种生物细胞氢载体骨架的制备方法，包括如下步骤：
A原料处理：
①将水生植物清洗，在1500r/min-3000r/min转速下粉碎成浆，经过60目-120目过滤滤出粗渣；②将滤得浆液通入紫外线玻璃管道中，在80W—100W功率下，杀菌10min-15min，杀掉杂菌，即得水生植物浆液，滤液收率为40％-60％；
B培育生长：
①按照水生植物浆液10％-20％、无菌小分子水60％-90％、载体蛋白酶1％-5％、连体蛋白1％-5％、受体蛋白1％-5％的质量百份比称取原料，上述原料的质量百份之和为100％；②将上述原料分别投入不绣钢保温容器内，该不绣钢保温容器分别设有上进料口、下出料口及带阀门的中取样口；③原料投入后，关闭进料口，开始缓慢升温至40℃，④通入氢气，通氢压力控制在0.001MPa-0.015MPa持续180min-500min，⑤控制温度25℃-40℃保持180min-200min后，通过中取样口取样，停止加热及通入氢气，最后通过下出料口将生物细胞氢载体骨架抽出，即得。
2.根据权利要求1所述的一种生物细胞氢载体骨架的制备方法，其特征是：所述步骤A中水生植物包括莲藕径叶、水葫芦、海藻、油藻。
3.一种生物细胞氢载体骨架组合物，包括如下组分及质量百分比：聚山梨醇脂2％-5％、脂醚1％-5％、脂肪醇环氧乙烷3％-5％、生物细胞氢载体骨架余量。
4.一种生物细胞氢载体骨架组合物，包括如下组分及质量百分比：脂醚1％-5％、椰油酰胺丙基甜菜碱0.5％-3％、脂肪醇环氧乙烷2％-5％、生物细胞氢载体骨架余量。
5.一种生物细胞氢载体骨架组合物，包括如下组分及质量百分比：十二月桂酸脂3％-5％、醇醚0.5％-3％、脂肪醇环氧乙烷2％-5％、生物细氢载体骨架余量。
6.一种生物细胞氢载体骨架组合物，包括如下组分及质量百分比：亚油酸甘油脂3％-6％、醇醚0.6％-2.5％、脂肪醇环氧乙烷2％-5％、生物细胞氢载体骨架余量。
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