CN114854489B - Chamomile essential oil and preparation method and application thereof - Google Patents

Chamomile essential oil and preparation method and application thereof Download PDF

Info

Publication number
CN114854489B
CN114854489B CN202210508704.4A CN202210508704A CN114854489B CN 114854489 B CN114854489 B CN 114854489B CN 202210508704 A CN202210508704 A CN 202210508704A CN 114854489 B CN114854489 B CN 114854489B
Authority
CN
China
Prior art keywords
parts
essential oil
methyl
methylethyl
methylene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202210508704.4A
Other languages
Chinese (zh)
Other versions
CN114854489A (en
Inventor
刘华
陈文丹
田宇
黄丛林
陈海霞
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beijing Academy of Agriculture and Forestry Sciences
Original Assignee
Beijing Academy of Agriculture and Forestry Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beijing Academy of Agriculture and Forestry Sciences filed Critical Beijing Academy of Agriculture and Forestry Sciences
Priority to CN202210508704.4A priority Critical patent/CN114854489B/en
Publication of CN114854489A publication Critical patent/CN114854489A/en
Application granted granted Critical
Publication of CN114854489B publication Critical patent/CN114854489B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B9/00—Essential oils; Perfumes
    • C11B9/0069—Heterocyclic compounds
    • C11B9/0073—Heterocyclic compounds containing only O or S as heteroatoms
    • C11B9/008—Heterocyclic compounds containing only O or S as heteroatoms the hetero rings containing six atoms
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B9/00—Essential oils; Perfumes
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B9/00—Essential oils; Perfumes
    • C11B9/0007—Aliphatic compounds
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B9/00—Essential oils; Perfumes
    • C11B9/0007—Aliphatic compounds
    • C11B9/0015—Aliphatic compounds containing oxygen as the only heteroatom
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B9/00—Essential oils; Perfumes
    • C11B9/0026—Essential oils; Perfumes compounds containing an alicyclic ring not condensed with another ring
    • C11B9/0034—Essential oils; Perfumes compounds containing an alicyclic ring not condensed with another ring the ring containing six carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B9/00—Essential oils; Perfumes
    • C11B9/0042—Essential oils; Perfumes compounds containing condensed hydrocarbon rings
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B9/00—Essential oils; Perfumes
    • C11B9/0042—Essential oils; Perfumes compounds containing condensed hydrocarbon rings
    • C11B9/0046—Essential oils; Perfumes compounds containing condensed hydrocarbon rings containing only two condensed rings
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B9/00—Essential oils; Perfumes
    • C11B9/0042—Essential oils; Perfumes compounds containing condensed hydrocarbon rings
    • C11B9/0046—Essential oils; Perfumes compounds containing condensed hydrocarbon rings containing only two condensed rings
    • C11B9/0049—Essential oils; Perfumes compounds containing condensed hydrocarbon rings containing only two condensed rings the condensed rings sharing two common C atoms
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B9/00—Essential oils; Perfumes
    • C11B9/0061—Essential oils; Perfumes compounds containing a six-membered aromatic ring not condensed with another ring
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B9/00—Essential oils; Perfumes
    • C11B9/02—Recovery or refining of essential oils from raw materials
    • C11B9/027—Recovery of volatiles by distillation or stripping
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • Dentistry (AREA)
  • Microbiology (AREA)
  • Agronomy & Crop Science (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Botany (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention belongs to the technical field of essential oil, and particularly relates to a coreopsis tinctoria essential oil, and a preparation method and application thereof. The flos Matricariae Chamomillae essential oil comprises components of flowers and leaves of flos Matricariae Chamomillae. The preparation method comprises the following steps: distilling flos Chrysanthemi and leaves with steam, and collecting distillate to obtain flos Chrysanthemi essential oil. The research shows that the Mao Hua chrysanthemum essential oil can effectively inhibit and/or kill the activity of the strain of xanthomonas, thereby preventing and treating black rot.

Description

Chamomile essential oil and preparation method and application thereof
Technical Field
The invention belongs to the technical field of essential oil, and particularly relates to a coreopsis tinctoria essential oil, and a preparation method and application thereof.
Background
The Chamomile (Chrysanthemum vestitum) is a wild herb of the genus Chrysanthemum of the family Compositae, a perennial herb, about 80 cm high. The stem is hard, the basal part is wooden, the dense-covered gray fluff is provided with axillary buds or axillary buds of the dense fluff are developed into short nutrition branches. Thick leaf quality and large change of leaf shape. The Mao Hua chrysanthemum grows on hillside, rock, valley, river bank, wasteland and loess hilly land with altitude of 340-1500 m, and is produced in the southwest part of Henan, the western part of Hubei province and the western part of Anhui province. The flower is used as a medicine, and has the effects of clearing heat and detoxicating, clearing liver and improving vision and the like. No report on flowers and leaves of Maohuaju as essential oil is known so far.
Black rot, a fungus, namely, trichuria subphylum palmitosis and phytophthora infestans. The black rot leaves are ill, yellow spots are generated at a plurality of leaves edges, the rear part of the leaf turns into V shape to develop inwards, the leaf veins turn black to be netty, and the whole leaf turns yellow and dry gradually. The symptoms are usually rot, black spots, yellowing of leaves and the like, which seriously affect the yield and quality of main vegetable crops, oil crops and landscape plants, and bring about serious loss. Some of the pathogenic bacteria have very limited bactericidal effects commonly used in application production, and are difficult to inhibit infection of horticultural crops.
Xanthomonas campestris (Xanthomonas campestris) is an important pathogenic bacterium capable of causing black rot of all crucifers (such as Chinese cabbage, cauliflower, etc.) on a global scale, and is a model bacterium for studying the interrelation between plants and pathogenic microorganisms, and is represented by Xanthomonas campestris, a pathogenic variety of Xanthomonas campestris (Xanthomonas campestris pv. Campestris, abbreviated as Xcc).
Disclosure of Invention
In order to solve the technical problems, the inventor of the invention provides a coreopsis tinctoria essential oil, a preparation method and application thereof based on the research of the field and through a great deal of experimental investigation.
Specifically, the invention firstly provides the Chamomile essential oil, which comprises the components of flowers and leaves of Chamomile.
According to the research of the invention, the flower and the leaf of the Mao Hua chrysanthemum are subjected to composite distillation, and the extracted essential oil component can effectively inhibit and/or kill the activity of the strain of xanthomonas, so that the black rot is prevented and treated.
Preferably, the coreopsis tinctoria essential oil comprises: camphor, 1-methyl-4- (prop-2-yloxy) benzene, borneol, 1, 8-cineole, stereoisomer-1-methyl-3-methylene-8- (1-methylethyl) -tricyclo [4.4.0.02,7] decane, (R) -4-methyl-1- (1-methylethyl) -3-cyclohexen-1-ol, S-myristal, (1S) -2, 2-dimethyl-3-methylene-bicyclo [2.2.1] heptane, 1-methyl-3- (1-methylethyl) -benzene, borneol acetate, (1S) -6, 6-dimethyl-2-methylene-bicyclo [3.1.1] heptane, 1, 7-trimethylbicyclo [2.2.1] hept-5-en-2-ol, sha Binxi, pinoleyl acetate, pinacolone.
Preferably, the coreopsis tinctoria essential oil comprises, in parts by weight: 13 to 16 parts of camphor, 6.5 to 8.5 parts of 1-methyl-4- (prop-2-yloxy) benzene, 5 to 7 parts of borneol, 4 to 6 parts of 1, 8-cineole, 2 to 4 parts of stereoisomer-1-methyl-3-methylene-8- (1-methylethyl) -tricyclo [4.4.0.02,7] decane, 3 to 5 parts of (R) -4-methyl-1- (1-methylethyl) -3-cyclohexen-1-ol, 2.5 to 4.5 parts of S-myristal, 2 to 4 parts of (1S) -2, 2-dimethyl-3-methylene-bicyclo [2.2.1] heptane, 2 to 4 parts of 1-methyl-3- (1-methylethyl) -benzene, 2 to 4 parts of borneol acetate, 2 to 4 parts of (1S) -6, 6-dimethyl-2-methylene-bicyclo [3.1.1] heptane, 2.1] hept-5-ene-2-ol, 2 to 35 parts of 1, 3 to 3 parts of pinone, 3 to 3 parts of pinolene acetate.
The Mao Hua chrysanthemum essential oil provided by the invention contains a large amount of specific terpenes, a proper amount of specific aromatic hydrocarbon and a small amount of specific aldehyde and ester, and the substances are compounded to endow the Mao Hua chrysanthemum essential oil with excellent bactericidal activity on a strain of Xanthomonas.
The invention also provides a preparation method of the coreopsis tinctoria essential oil, which comprises the following steps:
distilling flos Chrysanthemi and leaves with steam, and collecting distillate to obtain flos Chrysanthemi essential oil. The method carries out compound extraction on the flowers and leaves of the coreopsis tinctoria by a steam distillation method, and has simple process and no pollution.
Preferably, the distillation time is 1 to 4 hours. At the above distillation times, it is ensured that the target product is distilled off substantially completely.
Preferably, the mass ratio of flowers and leaves of the Maohuaju is 1:3-5. The obtained Chamomile essential oil has further improved antibacterial effect on Xanthomonas strains.
The invention also provides application of the Mao Hua chrysanthemum essential oil in inhibiting and/or killing activity of a strain of xanthomonas.
The invention also provides application of the Mao Hua chrysanthemum essential oil in preventing and treating black rot caused by a strain of xanthomonas.
Preferably, the strain of Xanthomonas is Xanthomonas campestris.
The invention also provides a compound essential oil with a sterilizing effect, which comprises the following components in parts by volume:
4-6 parts of the Maohuaju essential oil, 2-4 parts of tea tree, 1-3 parts of cinnamon and 0.5-1.5 parts of thyme.
The invention also provides application of the compound essential oil in inhibiting and/or killing activity of a xanthomonas strain, preferably xanthomonas campestris.
The inventor of the invention reasonably combines the flos chrysanthemi indici essential oil with tea tree, cinnamon and thyme to play a role in synergy, and compared with the single flos chrysanthemi essential oil, the obtained compound essential oil has more efficient antibacterial/sterilizing effects on the strain of xanthomonas, especially xanthomonas campestris.
Drawings
Fig. 1 is a diagram for verifying the antibacterial effect of Mao Hua chrysanthemum essential oil against xanthomonas campestris in the embodiment of the invention, wherein A is Mao Hua chrysanthemum essential oil treatment group and B is CK control group.
Fig. 2 is a graph for verifying the bactericidal effect of Mao Hua chrysanthemum essential oil against xanthomonas campestris in the embodiment of the invention, wherein A1-A3 are Mao Hua chrysanthemum essential oil treatment groups, and A4 is a CK control group.
Detailed Description
The present invention is described in further detail below with reference to specific examples, but is not intended to limit the scope of the present invention.
The experimental methods used in the following examples are conventional methods unless otherwise specified. The experimental materials and related equipment used in the examples below, unless otherwise specified, are all commercially available.
Example 1
Example 1 provides a coreopsis tinctoria essential oil, which is prepared by the following steps:
3663g of the flower and leaf parts of Maohuaju (mass ratio of flower to leaf is 1:4) and 7326ml of water are added to an essential oil hydrosol extraction apparatus, and heated by an induction cooker. Heating to boil with 2000W, then adjusting to 1200W, opening condensate water in a condenser tube, heating for distillation until no essential oil is separated out, and collecting distillate to obtain flos Matricariae Chamomillae essential oil with an essential oil extraction rate of 2.87%.
Analysis of the components of the obtained Maohuaju essential oil:
1.1 chromatographic Mass Spectrometry acquisition conditions
HS-SPME extraction conditions: shaking for 5min at a constant temperature of 60 ℃, inserting a 120 mu mDVB/CWR/PDMS extraction head into a sample headspace bottle, extracting for 15min at the headspace, analyzing for 5min at 250 ℃, and then performing GC-MS separation and identification. The extraction head was aged at 250℃for 5min in Fiber Conditioning Station before sampling.
Chromatographic conditions: the chromatographic column is a DB-5MS capillary column (30 m×0.25mm×0.25 μm), carrier gas: he (99.999%);
constant flow rate of 1.2mL/min, sample injection at 250 ℃ without split injection, and solvent delay of 3.5min. Heating program: the initial temperature of the column is 40 ℃, the column is kept for 3.5min, the temperature is increased to 100 ℃ at 10 ℃/min, the temperature is increased to 180 ℃ at 7 ℃/min, and the temperature is increased to 280 ℃ at 25 ℃/min, and the column is kept for 5min.
Mass spectrometry conditions: electron bombardment ion source (EI source), ion source temperature 230 deg.C, quaternary rod temperature 150 deg.C, mass spectrum interface temperature 280 deg.C, electron energy 70eV, scanning mode of selecting ion detection mode (SIM), qualitative and quantitative example accurate scanning (GB 23200.8-2016)
1.2 analysis results of the Components
The main components and the relative contents of the obtained Maohuaju essential oil are shown in table 1.
TABLE 1
A total of 562 species were detected. Wherein the content is 36 in total and is more than 0.5 percent, and the content is 80.64 percent of the total essential oil.
Verification experiment of the function of the Chamomile essential oil: xanthomonas campestris pathogenic variants
Selecting Xanthomonas campestris wild oilThe concentration of pathogenic variety of vegetable (XCC) is 3×10 8 100 mu L of CFU/ml bacterial liquid is uniformly coated on an LB plate without antibiotics, a sterilized 6mm filter paper sheet is added to the center of the plate, and finally 10 mu L of the Mao Hua chrysanthemum essential oil stock solution of the example 1 is added to the filter paper sheet, and a filter paper sheet culture dish without essential oil is used as a control for culturing for 24 hours at 28 ℃. Three groups are respectively arranged in the treatment group and the control group of the Mao Hua chrysanthemum essential oil.
1. Essential oil antibacterial effect
After 24 hours of incubation at 28℃the colony growth was as shown in FIG. 1 below. The obvious antibacterial zone appears in the treatment group of the Mao Hua chrysanthemum essential oil, and the CK control group has no antibacterial zone, which shows that the Mao Hua chrysanthemum essential oil has a certain inhibition effect on the growth of the pathogenic varieties of the Xanthomonas campestris.
The width of the antibacterial zone of the coreopsis tinctoria essential oil treatment group is respectively as follows: 21.61mm, 25.51mm, 21.45mm, average 22.86.+ -. 1.88mm.
2. Essential oil sterilizing effect
And (3) respectively scribing culture mediums (CK) in the bacteriostatic zone area and the non-bacteriostatic zone area of the coreopsis tinctoria essential oil treatment group, scribing on the new culture mediums, and then culturing for 24 hours in a constant-temperature incubator at 28 ℃.
The results are shown in FIG. 2, and after the culture medium selected from the zone of inhibition zone is cultured in the new culture medium, no colony grows (see A1-A3 in FIG. 2); while the medium selected from the zone of non-zone of inhibition, after streaking on the new medium, the bacteria can grow normally and form colonies (see A4 in FIG. 2). It is explained that in the region where the coreopsis tinctoria essential oil of example 1 acts, bacteria are killed or growth activity is affected.
While the invention has been described in detail in the foregoing general description, embodiments and experiments, it will be apparent to those skilled in the art that modifications and improvements can be made thereto. Accordingly, such modifications or improvements may be made without departing from the spirit of the invention and are intended to be within the scope of the invention as claimed.

Claims (9)

1. Use of an essential oil of coreopsis tinctoria for inhibiting and/or killing the activity of a strain of xanthomonas, characterized in that the essential oil of coreopsis tinctoria comprises the components of flowers and leaves of coreopsis tinctoria;
the strain of Xanthomonas is Xanthomonas campestris;
the preparation method of the Maohuaju essential oil comprises the following steps:
distilling flos Chrysanthemi and leaves with steam, and collecting distillate to obtain flos Chrysanthemi essential oil;
the distillation time is 1-4 hours;
the mass ratio of flowers to leaves of the Maohuaju is 1:3-5.
2. The use according to claim 1, wherein said coreopsis tinctoria essential oil comprises: camphor, 1-methyl-4- (prop-2-yloxy) benzene, borneol, 1, 8-cineole, stereoisomer-1-methyl-3-methylene-8- (1-methylethyl) -tricyclo [4.4.0.02,7] decane, (R) -4-methyl-1- (1-methylethyl) -3-cyclohexen-1-ol, S-myristal, (1S) -2, 2-dimethyl-3-methylene-bicyclo [2.2.1] heptane, 1-methyl-3- (1-methylethyl) -benzene, borneol acetate, (1S) -6, 6-dimethyl-2-methylene-bicyclo [3.1.1] heptane, 1, 7-trimethylbicyclo [2.2.1] hept-5-en-2-ol, sha Binxi, pinoleyl acetate, pinacolone.
3. The use according to claim 1, characterized in that said coreopsis tinctoria essential oil comprises, in parts by weight: 13-16 parts of camphor, 6.5-8.5 parts of 1-methyl-4- (prop-2-yloxy) benzene, 5-7 parts of borneol, 4-6 parts of 1, 8-cineole, 3-5 parts of stereoisomer-1-methyl-3-methylene-8- (1-methylethyl) -tricyclo [4.4.0.02,7] decane, 2.5-4.5 parts of (R) -4-methyl-1- (1-methylethyl) -3-cyclohexen-1-ol, 2-4 parts of S-myristal, (1S) -2, 2-dimethyl-3-methylene-bicyclo [2.2.1] heptane, 2-4 parts of 1-methyl-3- (1-methylethyl) -benzene, 2-4 parts of borneol acetate, (1S) -6, 6-dimethyl-2-methylene-bicyclo [3.1.1] heptane, 2, 7-trimethyl bicyclo [ 2.1] hept-5-oxa-2-ene-2, 2.1] heptane, 1-4 parts of pinone, 1-methyl-3-pine, and 1-pine.
4. The use of an essential oil of coreopsis tinctoria for controlling black rot caused by a strain of xanthomonas, characterized in that the essential oil of coreopsis tinctoria comprises the components of flowers and leaves of coreopsis tinctoria;
the strain of Xanthomonas is Xanthomonas campestris;
the preparation method of the Maohuaju essential oil comprises the following steps:
distilling flos Chrysanthemi and leaves with steam, and collecting distillate to obtain flos Chrysanthemi essential oil;
the distillation time is 1-4 hours;
the mass ratio of flowers to leaves of the Maohuaju is 1:3-5.
5. The use according to claim 4, wherein said coreopsis tinctoria essential oil comprises: camphor, 1-methyl-4- (prop-2-yloxy) benzene, borneol, 1, 8-cineole, stereoisomer-1-methyl-3-methylene-8- (1-methylethyl) -tricyclo [4.4.0.02,7] decane, (R) -4-methyl-1- (1-methylethyl) -3-cyclohexen-1-ol, S-myristal, (1S) -2, 2-dimethyl-3-methylene-bicyclo [2.2.1] heptane, 1-methyl-3- (1-methylethyl) -benzene, borneol acetate, (1S) -6, 6-dimethyl-2-methylene-bicyclo [3.1.1] heptane, 1, 7-trimethylbicyclo [2.2.1] hept-5-en-2-ol, sha Binxi, pinoleyl acetate, pinacolone.
6. The use according to claim 4, characterized in that said coreopsis tinctoria essential oil comprises, in parts by weight: 13-16 parts of camphor, 6.5-8.5 parts of 1-methyl-4- (prop-2-yloxy) benzene, 5-7 parts of borneol, 4-6 parts of 1, 8-cineole, 3-5 parts of stereoisomer-1-methyl-3-methylene-8- (1-methylethyl) -tricyclo [4.4.0.02,7] decane, 2.5-4.5 parts of (R) -4-methyl-1- (1-methylethyl) -3-cyclohexen-1-ol, 2-4 parts of S-myristal, (1S) -2, 2-dimethyl-3-methylene-bicyclo [2.2.1] heptane, 2-4 parts of 1-methyl-3- (1-methylethyl) -benzene, 2-4 parts of borneol acetate, (1S) -6, 6-dimethyl-2-methylene-bicyclo [3.1.1] heptane, 2, 7-trimethyl bicyclo [ 2.1] hept-5-oxa-2-ene-2, 2.1] heptane, 1-4 parts of pinone, 1-methyl-3-pine, and 1-pine.
7. The application of compound essential oil with sterilization effect in inhibiting and/or killing the activity of xanthomonas strains is characterized in that the compound essential oil comprises the following components in parts by volume:
4-6 parts of coreopsis tinctoria essential oil, 2-4 parts of tea tree, 1-3 parts of cinnamon and 0.5-1.5 parts of thyme;
the flos Matricariae Chamomillae essential oil comprises the components of flowers and leaves of flos Matricariae Chamomillae;
the strain of Xanthomonas is Xanthomonas campestris;
the preparation method of the Maohuaju essential oil comprises the following steps:
distilling flos Chrysanthemi and leaves with steam, and collecting distillate to obtain flos Chrysanthemi essential oil;
the distillation time is 1-4 hours;
the mass ratio of flowers to leaves of the Maohuaju is 1:3-5.
8. The use according to claim 7, wherein said coreopsis tinctoria essential oil comprises: camphor, 1-methyl-4- (prop-2-yloxy) benzene, borneol, 1, 8-cineole, stereoisomer-1-methyl-3-methylene-8- (1-methylethyl) -tricyclo [4.4.0.02,7] decane, (R) -4-methyl-1- (1-methylethyl) -3-cyclohexen-1-ol, S-myristal, (1S) -2, 2-dimethyl-3-methylene-bicyclo [2.2.1] heptane, 1-methyl-3- (1-methylethyl) -benzene, borneol acetate, (1S) -6, 6-dimethyl-2-methylene-bicyclo [3.1.1] heptane, 1, 7-trimethylbicyclo [2.2.1] hept-5-en-2-ol, sha Binxi, pinoleyl acetate, pinacolone.
9. The use according to claim 7, characterized in that said coreopsis tinctoria essential oil comprises, in parts by weight: 13-16 parts of camphor, 6.5-8.5 parts of 1-methyl-4- (prop-2-yloxy) benzene, 5-7 parts of borneol, 4-6 parts of 1, 8-cineole, 3-5 parts of stereoisomer-1-methyl-3-methylene-8- (1-methylethyl) -tricyclo [4.4.0.02,7] decane, 2.5-4.5 parts of (R) -4-methyl-1- (1-methylethyl) -3-cyclohexen-1-ol, 2-4 parts of S-myristal, (1S) -2, 2-dimethyl-3-methylene-bicyclo [2.2.1] heptane, 2-4 parts of 1-methyl-3- (1-methylethyl) -benzene, 2-4 parts of borneol acetate, (1S) -6, 6-dimethyl-2-methylene-bicyclo [3.1.1] heptane, 2, 7-trimethyl bicyclo [ 2.1] hept-5-oxa-2-ene-2, 2.1] heptane, 1-4 parts of pinone, 1-methyl-3-pine, and 1-pine.
CN202210508704.4A 2022-05-10 2022-05-10 Chamomile essential oil and preparation method and application thereof Active CN114854489B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210508704.4A CN114854489B (en) 2022-05-10 2022-05-10 Chamomile essential oil and preparation method and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210508704.4A CN114854489B (en) 2022-05-10 2022-05-10 Chamomile essential oil and preparation method and application thereof

Publications (2)

Publication Number Publication Date
CN114854489A CN114854489A (en) 2022-08-05
CN114854489B true CN114854489B (en) 2024-03-22

Family

ID=82636802

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210508704.4A Active CN114854489B (en) 2022-05-10 2022-05-10 Chamomile essential oil and preparation method and application thereof

Country Status (1)

Country Link
CN (1) CN114854489B (en)

Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4569841A (en) * 1983-06-10 1986-02-11 Chevron Research Company Erwinia herbicola strain that inhibits pathogenic plant bacteria
WO2013054959A1 (en) * 2011-10-13 2013-04-18 덕성여자대학교 산학협력단 Antibacterial composition extracted from chrysanthemum morifolium flower
CN107982298A (en) * 2017-12-19 2018-05-04 中国农业科学院烟草研究所 The extracting method of wormwood essential oil and the application in terms of phytopathogen is suppressed
CN108715773A (en) * 2018-05-30 2018-10-30 叶于诚 The extraction process of shaddock peel essential oil, the application of shaddock peel essential oil and application method
KR20190009177A (en) * 2017-07-18 2019-01-28 중앙대학교 산학협력단 ScpB Protein in Xanthomonas campestris pv. vesicatoria and the use thereof
CN109601559A (en) * 2019-01-07 2019-04-12 贵州省烟草公司遵义市公司 It is a kind of prevent and treat phytophthora mother chrysanthemum essential oil and its application
CN110643527A (en) * 2019-09-06 2020-01-03 华南农业大学 Degradation strain of pesticide tetramethrin and application thereof
CN110859872A (en) * 2019-12-04 2020-03-06 湖北创界生物科技有限公司 Anti-inflammatory and bactericidal essential oil composition, and preparation method and application thereof
CN112076240A (en) * 2020-09-23 2020-12-15 北京农业生物技术研究中心 Essential oil for branches and leaves of Taisui chrysanthemum and application thereof
CN112121076A (en) * 2020-09-23 2020-12-25 北京农业生物技术研究中心 Chamomile essential oil and application thereof
CN112263602A (en) * 2020-10-29 2021-01-26 北京农业生物技术研究中心 Minghuang chrysanthemum essential oil and application thereof
CN112680279A (en) * 2020-12-09 2021-04-20 重庆市日用化学工业研究所 Natural medicinal fragrant plant composite essential oil and essential oil soap with sterilizing and anti-inflammation functions and preparation method thereof
WO2021094552A1 (en) * 2019-11-15 2021-05-20 Universiteit Gent Sunflower bark extract and uses thereof

Patent Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4569841A (en) * 1983-06-10 1986-02-11 Chevron Research Company Erwinia herbicola strain that inhibits pathogenic plant bacteria
WO2013054959A1 (en) * 2011-10-13 2013-04-18 덕성여자대학교 산학협력단 Antibacterial composition extracted from chrysanthemum morifolium flower
KR20190009177A (en) * 2017-07-18 2019-01-28 중앙대학교 산학협력단 ScpB Protein in Xanthomonas campestris pv. vesicatoria and the use thereof
CN107982298A (en) * 2017-12-19 2018-05-04 中国农业科学院烟草研究所 The extracting method of wormwood essential oil and the application in terms of phytopathogen is suppressed
CN108715773A (en) * 2018-05-30 2018-10-30 叶于诚 The extraction process of shaddock peel essential oil, the application of shaddock peel essential oil and application method
CN109601559A (en) * 2019-01-07 2019-04-12 贵州省烟草公司遵义市公司 It is a kind of prevent and treat phytophthora mother chrysanthemum essential oil and its application
CN110643527A (en) * 2019-09-06 2020-01-03 华南农业大学 Degradation strain of pesticide tetramethrin and application thereof
WO2021094552A1 (en) * 2019-11-15 2021-05-20 Universiteit Gent Sunflower bark extract and uses thereof
CN110859872A (en) * 2019-12-04 2020-03-06 湖北创界生物科技有限公司 Anti-inflammatory and bactericidal essential oil composition, and preparation method and application thereof
CN112076240A (en) * 2020-09-23 2020-12-15 北京农业生物技术研究中心 Essential oil for branches and leaves of Taisui chrysanthemum and application thereof
CN112121076A (en) * 2020-09-23 2020-12-25 北京农业生物技术研究中心 Chamomile essential oil and application thereof
CN112263602A (en) * 2020-10-29 2021-01-26 北京农业生物技术研究中心 Minghuang chrysanthemum essential oil and application thereof
CN112680279A (en) * 2020-12-09 2021-04-20 重庆市日用化学工业研究所 Natural medicinal fragrant plant composite essential oil and essential oil soap with sterilizing and anti-inflammation functions and preparation method thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
毛华菊精油化学成分研究;王国亮等;武汉植物学研究;第13卷(第4期);383-384 *
赵乃昕等.医学细菌名称及分类鉴定（第三版）.山东大学出版社,2013,435-438. *

Also Published As

Publication number Publication date
CN114854489A (en) 2022-08-05

Similar Documents

Publication Publication Date Title
CN110150333B (en) Cinnamomum longepaniculatum leaf essential oil bacteriostasis method
CN104762230B (en) Prevent and treat Shewanella alga and its application of crop storage phase Aspergillus flavus and toxin
CN102676420B (en) Pseudomonas putida strain for bio-control of Tobacco mosaic virus
CN114854488B (en) Wild chrysanthemum essential oil and preparation method and application thereof
CN112574892B (en) Mucor circinelloides for promoting root system development of red sage root and tanshinone synthesis and its use
CN114854489B (en) Chamomile essential oil and preparation method and application thereof
CN103290065A (en) Method for preparing borneol by using microorganism separated from natural world
CN110438011B (en) Cladosporium tenuissimum and application thereof in promoting synthesis of effective components of root system of salvia miltiorrhiza
CN114774199B (en) Extraction method of natural perfume
Zhang et al. Effects of crop development on the emission of volatiles in leaves of Lycopersicon esculentum and its inhibitory activity to Botrytis cinerea and Fusarium oxysporum
Khorasani et al. Beneficial effects of endophytic fungi inoculation on tanshinones and phenolic compounds of Salvia abrotanoides
CN108795773A (en) Moschus trichoderma strain and its fragrance of preparation
CN108795772A (en) Moschus trichoderma strain and its fragrance of preparation
CN104830717B (en) Red sage root endophyte with induction tanshin polyphenolic acid B summation and application thereof
CN108795771A (en) Moschus trichoderma strain and its fragrance of preparation
CN108064887B (en) Application of cleome robusta in inhibiting activity of pathogenic bacteria of plant soil-borne diseases
CN111838197B (en) Active ingredient of marigold rhizome and application thereof in preventing and treating ralstonia solanacearum
CN113647414B (en) Aquilaria plant edgeworthia chrysantha promoter as well as preparation method and application thereof
Donovan et al. Inhibitory effects of essential oil components extracted from celery (Apium graveolens) on the growth of Septoria apiicola, causal agent of leaf spot disease
CN112877222B (en) Strain for antagonizing sclerotinia rot of asarum and application thereof
Yao Preliminary assessment of rapid agarwood-produced technique using artificial infusion method in taiwan
CN116965407B (en) Application of artemisia rupestris extract in preparation of preparation for preventing and treating tobacco black shank
CN117941700B (en) Botanical fungicide for preventing and treating black spot of pseudo-ginseng
CN108849960B (en) Compound essential oil preparation for resisting sweet potato chayote, preparation method thereof and application thereof in preventing and treating black spot of sweet potato
Zamfirache et al. Research regarding the volatile oils composition for Ocimum basilicum L. and their possible phytotherapeutic effects

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant