CN113647414B - Aquilaria plant edgeworthia chrysantha promoter as well as preparation method and application thereof - Google Patents
Aquilaria plant edgeworthia chrysantha promoter as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of aquilaria resinifera and discloses an aquilaria resinifera growth promoter, and a preparation method and application thereof. The agarwood plant edgeworthia chrysantha promoter comprises the following components: agilawood water-soluble extract, lactobacillus plantarum bacterial liquid, saccharides and organic acid. The agilawood water-soluble extract and the lactobacillus plantarum strain are used as main raw materials of the agilawood formation promoter for the agilawood plants, so that the biocompatibility is good, the residues of heavy metals or other toxic chemical agents in the agilawood and the phenomenon that the normal growth and metabolism of the agilawood plants are seriously hindered are avoided, and the formation promoter is green, environment-friendly, safe and reliable.
Description
Technical Field
The invention belongs to the technical field of aquilaria resinatum, and particularly relates to an aquilaria resinatum promoter as well as a preparation method and application thereof.
Background
Agilawood is a famous and precious aromatic medicine which is reputed in the world, various agilawood plants are original plants for obtaining agilawood medicinal materials, and the main agilawood medicinal material in China is mainly Aquilaria sinensis (Lour.) Gilg. The natural agilawood formation period of the agilawood plant is long, the agilawood formation probability is low, and the agilawood is obtained by adopting an artificial agilawood formation method mostly. The method for promoting the aquilaria resinifera to produce the aquilaria resinifera is a mainstream technical means in the agilawood industry at present by an infusion method, the aquilaria resinifera promoter is a main factor directly influencing the aquilaria resinifera production of the aquilaria resinifera by the infusion method, and the problems that heavy metals or other toxic chemical agent residues exist in the conventional aquilaria resinifera promoter in the aquilaria resinifera industry, the normal growth and metabolism of the aquilaria resinifera plant are seriously hindered due to the input of the aquilaria resinifera promoter, the detection indexes specified in national formulary such as linalool-soluble extract and agarotetraol obtained by promoting the aquilaria resinifera are not high, and the like exist in the conventional aquilaria resinifera promoter.
The agilawood essential oil (volatile oil) is a main component of volatile efficacy of agilawood and is an important product form in the current agilawood medicinal material deep processing industry. At present, the existing methods such as traditional steam distillation of preparation of agalloch eaglewood essential oil also have the novel technical method such as the extensive supercritical fluid extraction of present application, but all have a problem, the extract after having extracted the essential oil directly abandons many times promptly, and this extract is mainly the non-volatile composition in the agalloch eaglewood medicinal material, does not carry out further utilization and directly abandons and causes the wasting of resources.
A plurality of scholars at home and abroad discover that the formation of the natural agilawood is closely related to the induction of fungi, and discover that some fungi can induce the agilawood plants to generate the agilawood. Lactobacillus plantarum is one of lactic acid bacteria, is one of common fermentation strains for fermenting food, and no report of using the Lactobacillus plantarum in agilawood edgeworthia chrysantha is found at present.
Disclosure of Invention
The invention aims at providing an aquilaria plant edgeworthia chrysantha promoter.
The second aspect of the present invention is to provide a method for preparing the agaric plant edgeworthia chrysantha promoter of the first aspect.
The third aspect of the present invention is to provide the use of the agaric plant edgeworthia chrysantha promoter in the agaric plant edgeworthia chrysantha of the first aspect.
The fourth aspect of the invention aims to provide a method for producing agilawood.
The fifth aspect of the invention aims to provide agilawood.
The sixth aspect of the invention aims at providing the application of the agilawood of the fifth aspect in preparing products.
The seventh aspect of the invention aims at providing agilawood essential oil.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
the invention provides a first aspect of an agilawood formation promoter, which comprises the following components: agilawood water-soluble extract, lactobacillus plantarum bacterial liquid, saccharides and organic acid.
Preferably, the agarwood plant edgeworthia chrysantha promoter comprises, by weight: 5 to 30 percent of agilawood water-soluble extract, 5 to 15 percent of lactobacillus plantarum bacterial liquid, 2 to 8 percent of saccharides and 0.5 to 3 percent of organic acid.
Further preferably, the agaric plant edgeworthia chrysantha promoter comprises, by weight: 10-30% of agilawood water-soluble extract, 5-10% of lactobacillus plantarum bacterial liquid, 2-5% of saccharides and 1-3% of organic acid.
Still more preferably, the agaric plant edgeworthia chrysantha promoter comprises, by weight: 20-30% of agilawood water-soluble extract, 5-8% of lactobacillus plantarum bacterial liquid, 3-5% of saccharides and 1-1.5% of organic acid.
Preferably, the preparation method of the agilawood water-soluble extract comprises the following steps: mixing the essential oil-extracted agilawood with water, and leaching to obtain the agilawood water-soluble extract.
Preferably, the mass volume ratio (g/mL) of the agilawood to the water is 1: (10-20).
Further preferably, the mass-to-volume ratio (g/mL) of the agilawood to the water is 1: (10-15).
Preferably, the temperature of the leaching is 90-100 ℃.
Preferably, the leaching time is 8-10 h.
Preferably, the preparation method of the lactobacillus plantarum bacterial liquid comprises the following steps: inoculating the lactobacillus plantarum into a culture medium, and culturing to obtain lactobacillus plantarum bacterial liquid.
Further preferably, the medium is MRS medium.
Further preferably, the temperature of the cultivation is 30 to 40 ℃.
Even more preferably, the temperature of the cultivation is 35-37 ℃.
Further preferably, the culturing time is 15-25 h.
More preferably, the lactobacillus plantarum bacterial solution is cultured until the bacterial solution concentration is 2 × 108-6×108CFU/mL。
Preferably, the sugar is one or more of white granulated sugar, glucose and sucrose.
Preferably, the organic acid is one or more of citric acid, tartaric acid, acetic acid, succinic acid and oxalic acid.
Preferably, the agarwood plant edgeworthia chrysantha promoter further comprises water, and the balance is water.
In a second aspect of the present invention, there is provided a method for preparing the agaric plant edgeworthia chrysantha promoter of the first aspect of the present invention, comprising the steps of: mixing the agilawood water-soluble extract, saccharides, lactobacillus plantarum bacterial liquid, organic acid and water to obtain the edgeworthia chrysantha promoter.
Preferably, the water-soluble extract of lignum Aquilariae Resinatum is dried before mixing.
Further preferably, the drying conditions of the agilawood water-soluble extract are as follows: drying at 60-80 deg.C.
In a third aspect of the present invention, there is provided a use of the agaric plant edgeworthia chrysantha promoter of the first aspect of the present invention in edgeworthia chrysantha of the agaric plant.
Preferably, the agarwood is selected from one of aquilaria sinensis, linaloe yunnanensis and linaloe majorana.
Further preferably, the aquilaria plant is aquilaria sinensis.
In a fourth aspect of the present invention, a method for producing agilawood is provided, wherein the agilawood production promoter of the first aspect of the present invention is added to an agilawood plant.
Preferably, the agaric plant edgeworthia chrysantha promoter of the first aspect of the present invention is introduced into the agaric plant by an infusion method.
Preferably, the agaric plant edgeworthia chrysantha promoter of the first aspect of the present invention is injected into the agaric plant using a bottle injection method or a bottle insertion method.
In a fifth aspect of the present invention, an agilawood is provided, which is obtained by the agilawood formation method according to the fourth aspect of the present invention.
Preferably, the content of the ethanol extract of the agilawood is 50.0% -60%.
Further preferably, the content of the ethanol extract of the agilawood is 57.76-60%.
Preferably, the content of the agaratetraol in the agilawood is 1.0% -2.0%.
Further preferably, the content of the agarotetraol in the agilawood is 1.13% -2.0%.
The sixth aspect of the invention provides the application of the agilawood of the fifth aspect in preparing products.
Preferably, the products include pharmaceuticals, essential oils, foods, cosmetics, daily chemicals, and crafts.
Further preferably, the handicraft is a conventional handicraft in industries such as pen containers, beads, wood carving ornaments and the like.
The seventh aspect of the invention provides agilawood essential oil which is refined from the agilawood of the fifth aspect.
Preferably, the linalool content of the agilawood essential oil is 1% -5%.
Further preferably, the linalool content of the agilawood essential oil is 2.16% -5%.
Preferably, the content of benzyl acetone in the agilawood essential oil is 0.1% -0.5%.
Further preferably, the content of benzyl acetone in the agilawood essential oil is 0.29% -0.5%.
Preferably, the aquilaria sinensis essential oil has an aquilaria sinensis aldehyde content of 7% -10%.
Further preferably, the aquilaria sinensis essential oil has an aquilaria sinensis aldehyde content of 8.35% -10%.
Preferably, the lactarius vellereus aldehyde content of the agilawood essential oil is 11% -15%.
Further preferably, the lactarius vellereus aldehyde content of the agilawood essential oil is 12.11% -15%.
Preferably, the alkene content of the agilawood essential oil is 10% -13.5%.
Further preferably, the alkene content of the agilawood essential oil is 12% -13.5%.
Preferably, the oleic acid content of the agilawood essential oil is 10% -13%.
Further preferably, the oleic acid content of the agilawood essential oil is 10.62% -13%.
The invention has the beneficial effects that: 1. according to the invention, the agilawood water-soluble extract and the lactobacillus plantarum are used as main raw materials of the aquilaria plant edgeworthia chrysantha promoter, so that the biocompatibility is better, the residues of heavy metals or other toxic chemical agents in agilawood and the phenomenon that the normal growth and metabolism of the agilawood are seriously hindered are avoided, and the aquilaria plant edgeworthia chrysantha promoter is green, environment-friendly, safe and reliable; 2. the agaric plant edgeworthia chrysantha promoter provided by the invention can effectively increase the biomass of chemical components of the agaric tree edgeworthia chrysantha, and improve the edgeworthia chrysantha success rate and the yield and quality of agilawood; 3. the agarwood plant edgeworthia chrysantha promoter provided by the invention can increase the biomass of volatile aroma substances in the agilawood essential oil, thereby improving the quality of the agilawood essential oil.
Drawings
FIG. 1 is a field view of inputting the edgeworthia chrysantha promoting agent into aquilaria sinensis.
FIG. 2 is a macroscopic view of the lignum Aquilariae Resinatum produced by the lignum Aquilariae Resinatum promoter.
Detailed Description
The present invention will now be described in detail with reference to specific examples, but the scope of the present invention is not limited thereto.
The materials, reagents and the like used in the present examples are commercially available materials and reagents unless otherwise specified. The lactobacillus plantarum is purchased from a Chinese microbial strain inquiry network, and the platform number is as follows: bio-60181, http:// www.biobw.org/China-strain/bio-60181. html.
Example 1
An agilawood formation promoter comprises 20 wt% of agilawood water-soluble extract, 8 wt% of lactobacillus plantarum bacterial liquid, 3 wt% of white granulated sugar, 1.5 wt% of vinegar and 67.5 wt% of purified water;
the preparation method of the agilawood water-soluble extract comprises the following steps: taking 25g of agilawood powder dried after extracting essential oil (the agilawood essential oil extraction method refers to the patent document, "distillation production method and equipment of agilawood essential oil" application No. CN201610212220.X example 1, agilawood fragments remained in a distillation pot in the step 2 are agilawood powder used by the invention), mixing the agilawood powder with distilled water (the mass-volume ratio of material liquid is 1:10), leaching at 95-100 ℃ for 8h, and filtering by adopting three layers of gauze to obtain an agilawood water-soluble extract;
the preparation method of the lactobacillus plantarum bacterial liquid comprises the following steps: inoculating Lactobacillus plantarum in MRS culture medium, culturing at 37 deg.C in anaerobic incubator for 18 hr to obtain 3 × 10 strain solution8CFU/mL lactobacillus plantarum solution.
The MRS culture medium is as follows: casein peptone 10.0g, beef extract 10.0g, yeast powder 5.0g, glucose 5.0g, sodium acetate 5.0g, diammonium citrate 2.0g, Tween 801.0 g, and K2HPO4 2.0g、MgSO4.7H2O 0.2g、MnSO4.H2O 0.05g、CaCO320.0g of agar and 15.0g of agar, diluting to 1.0L, and adjustingThe pH was 6.8.
The preparation method of the agaric plant edgeworthia chrysantha promoter comprises the following specific steps:
removing water from lignum Aquilariae Resinatum water soluble extract by rotary evaporator (YRE-301 type), oven drying at 60 deg.C, adding white sugar, mixing, pulverizing to powder, sieving with 100 mesh sieve, adding Lactobacillus plantarum liquid, edible vinegar and purified water, and mixing to obtain lignum Aquilariae Resinatum plant lignum Aquilariae Resinatum formation promoter.
Example 2
An agilawood formation promoter for a plant of the genus agilawood comprises 30 wt% of agilawood water-soluble extract, 10 wt% of lactobacillus plantarum bacterial liquid, 5 wt% of white granulated sugar, 3 wt% of table vinegar and 52 wt% of purified water;
the preparation method of the agilawood water-soluble extract comprises the following steps: taking 25g of agilawood powder dried after extracting essential oil (the agilawood essential oil extraction method refers to the patent document, "distillation production method and equipment of agilawood essential oil" application No. CN201610212220.X example 1, agilawood fragments remained in a distillation pot in the step 2 are agilawood powder used by the invention), mixing the agilawood powder with distilled water (the mass-volume ratio of material liquid is 1:10), leaching at 95-100 ℃ for 8h, and filtering by adopting three layers of gauze to obtain an agilawood water-soluble extract;
the preparation method of the lactobacillus plantarum bacterial liquid comprises the following steps: inoculating Lactobacillus plantarum in MRS culture medium, culturing at 37 deg.C in anaerobic incubator for 21 hr to obtain bacterial liquid with concentration of 4 × 108CFU/mL lactobacillus plantarum bacterial liquid;
the MRS culture medium is as follows: casein peptone 10.0g, beef extract 10.0g, yeast powder 5.0g, glucose 5.0g, sodium acetate 5.0g, diammonium citrate 2.0g, Tween 801.0 g, and K2HPO4 2.0g、MgSO4.7H2O 0.2g、MnSO4.H2O 0.05g、CaCO320.0g of agar and 15.0g of agar, and adjusting the pH to 6.8 after the constant volume of 1.0L.
The preparation method of the agaric plant edgeworthia chrysantha promoter comprises the following specific steps:
removing water from the water-soluble extract of lignum Aquilariae Resinatum by rotary evaporator (YRE-301 type), oven drying at 60 deg.C, adding white sugar, mixing, pulverizing, sieving with 100 mesh sieve, adding Lactobacillus plantarum bacterial liquid, edible vinegar and purified water, and mixing to obtain lignum Aquilariae Resinatum plant lignum Aquilariae Resinatum promoter.
Example 3
An agilawood formation promoter comprises, by weight, 10% of agilawood water-soluble extract, 5% of lactobacillus plantarum bacterial liquid, 2% of white granulated sugar, 1% of vinegar and 82% of purified water;
the preparation method of the agilawood water-soluble extract comprises the following steps: taking 25g of agilawood powder dried after extracting essential oil (the agilawood essential oil extraction method refers to the patent document, "distillation production method and equipment of agilawood essential oil" application No. CN201610212220.X example 1, agilawood fragments remained in a distillation pot in the step 2 are agilawood powder used by the invention), mixing the agilawood powder with distilled water (the mass-volume ratio of material liquid is 1:10), leaching at 95-100 ℃ for 8h, and filtering by adopting three layers of gauze to obtain an agilawood water-soluble extract;
the preparation method of the lactobacillus plantarum bacterial liquid comprises the following steps: inoculating Lactobacillus plantarum in MRS culture medium, culturing in 37 deg.C anaerobic incubator for 24 hr to obtain 5 × 10 bacterial liquid8CFU/mL lactobacillus plantarum bacterial liquid;
the MRS culture medium is: casein peptone 10.0g, beef extract 10.0g, yeast powder 5.0g, glucose 5.0g, sodium acetate 5.0g, diammonium citrate 2.0g, Tween 801.0 g, and K2HPO4 2.0g、MgSO4.7H2O 0.2g、MnSO4.H2O 0.05g、CaCO320.0g of agar and 15.0g of agar, and adjusting the pH to 6.8 after the constant volume of 1.0L.
The preparation method of the agaric plant edgeworthia chrysantha promoter comprises the following specific steps:
removing water from the water-soluble extract of lignum Aquilariae Resinatum with rotary evaporator (YRE-301 type), oven drying at 60 deg.C, adding white sugar, mixing, pulverizing, sieving with 100 mesh sieve, adding Lactobacillus plantarum bacterial liquid, edible vinegar and purified water, and mixing to obtain lignum Aquilariae Resinatum plant lignum Aquilariae Resinatum formation promoter.
Effects of the embodiment
1. Inoculating lignum Aquilariae Resinatum plant lignum Aquilariae Resinatum promoter (infusion method)
(1) The aquilaria sinensis trees (2 aquilaria sinensis trees in total, wherein 1 aquilaria sinensis fruiting promoter is inoculated on 1 aquilaria sinensis fruiting tree, and 1 aquilaria sinensis fruiting promoter is not inoculated on 1 aquilaria sinensis fruiting tree) which are planted in situ, have large crown, luxuriant leaves, intact bark and full illumination and are 8-year-old trunks and 12cm in diameter at chest are selected. Clearing the shade around the aquilaria sinensis tree, weeding and applying fertilizer (poultry and livestock excrement, peanut bran and the like) for a period of time, so that the aquilaria sinensis tree grows vigorously and stores enough nutrients.
(2) Opening a hole: a hole is drilled at 40cm above the ground from the root of the aquilaria sinensis, a drill bit is used for a percussion drill with the specification of 0.6mm, holes with the diameter of 0.7cm and the depth of 8cm are drilled in the trunk according to different breast diameters, and 2 holes are drilled in each tree.
(3) Transfusion: the agaric plant agaric accelerant prepared in the example 1 is transferred into an infusion bag and hung on a higher trunk, then an infusion needle is inserted into a hole formed in a tree, so that the agaric plant agaric accelerant flows from the infusion bag through a conduit and flows into the needle to be continuously input into the aquilaria sinensis tree, and a preservative film is used for binding and sealing (as shown in figure 1), so that infectious microbe infection, insect and ant hazards, and agaric agent outflow and rainwater scouring are prevented. After the infusion is finished, the needle head is pulled out of the hole, and the situations of tree leaf falling and tree trunk death are not observed within ten days after the infusion is finished.
(4) Nursing: after one month of opening the holes, fertilizing the aquilaria sinensis by using feces of livestock and poultry, peanut bran and the like, topdressing after half a year, and simultaneously paying attention to insect prevention.
(5) Agilawood collection: agilawood can be collected 12 months after the artificial promotion of agilawood formation treatment. Before agilawood is harvested, the phloem sieve tube channel is partially or completely cut off at one or more positions on the harvested trunk or branch, so that the transmission of nutrient substances to a root system is reduced, the nutrient substances are accumulated at the harvesting position, and the quality of the agilawood is obviously improved; selecting a tree trunk with aromatic resin when harvesting, cutting and removing white wood parts and rotten parts by a sharp knife, and drying in the shade to obtain the agilawood.
2. Agilawood essential oil refining method
The agilawood essential oil is extracted by taking the collected agilawood as a raw material (taking natural agilawood, namely agilawood without inoculating an agilawood plant fragrance-forming agent as a comparative example) according to a volatile oil extraction (steam distillation method) method recorded in Chinese pharmacopoeia (2020 edition).
3. Detection method for detection indexes of agilawood and agilawood essential oil
Inspecting and judging agilawood according to the content of an ethanol extract, a color reaction, a thin-layer chromatography, a high performance liquid characteristic chromatography and other methods recorded in national forestry industry standard LY/T2904-2017 agilawood;
detecting the content of the linalool in the agilawood according to a method recorded in Chinese pharmacopoeia (2020 edition);
the composition and content of the agilawood essential oil (volatile oil) are measured according to the method recorded in Chinese pharmacopoeia (2020 edition).
4. Analysis of results
The aquilaria resinifera thunb growth promoter prepared in the example 1 is input into an aquilaria sinensis tree, and the situation that leaves fall off and a trunk dies is not observed within ten days after infusion is finished, so that the aquilaria resinifera thunb growth promoter provided by the embodiment can not influence the normal growth and metabolism of the aquilaria sinensis tree. Harvesting lignum Aquilariae Resinatum 12 months after artificial promotion of lignum Aquilariae Resinatum production, as shown in FIG. 2, the dark brown oily matter and yellow brown color-changing xylem formed in lignum Aquilariae Resinatum tree inoculated with lignum Aquilariae Resinatum production promoter, i.e. lignum Aquilariae Resinatum produced by radix Aquilariae Resinatum, while the lignum Aquilariae Resinatum tree not inoculated with lignum Aquilariae Resinatum production promoter has no lignum Aquilariae Resinatum.
By detecting the contents of the ethanol extract, the agarotetraol and the like in the agilawood, the result is shown in table 1, the content of the ethanol extract in the agilawood obtained in example 1 is 57.76%, the content of the agarotetraol is 1.13%, and the content of the agarotetraol is far greater than national forestry industry standard LY/T2904-2017 and relevant standards of Chinese pharmacopoeia (the content of the ethanol extract is more than or equal to 10.0%, and the content of the agarotetraol is more than or equal to 0.10%), the prepared agilawood is cherry red through color reaction, thin-layer chromatography and high performance liquid characteristic map analysis, fluorescent spots with the same color are displayed on positions corresponding to the chromatography of the agilawood control sample, and 6 characteristic peaks which are the same as those on the high-phase liquid characteristic map of the control sample are displayed. Compared with the patent of invention granted under patent number ZL 201610373562.X), the obtained ethanol extract of lignum Aquilariae Resinatum and linalool are 18.58% and 0.19% respectively, which are obviously lower than the ethanol extract of lignum Aquilariae Resinatum and linalool content obtained in example 1, which indicates that the lignum Aquilariae Resinatum formation promoter prepared in example 1 is input into lignum Aquilariae Resinatum tree for formation of lignum Aquilariae Resinatum, so that the formation success rate and formation efficiency of radix Aquilariae Resinatum can be effectively improved, and the yield and quality of lignum Aquilariae Resinatum are improved.
By comparing the example 1 with natural agilawood (naturally formed agilawood without artificial treatment), the main components and the contents of the essential oils of agilawood extracted from agilawood are obtained, and the results are shown in table 2, and the contents of agarol, aquilaria aldehyde, villous Lactarius aldehyde, alkene and oleic acid in the essential oil of agilawood extracted from example 1 are obviously higher than those of the essential oil of agilawood extracted from natural agilawood, which indicates that the agilawood plant agilawood formation promoter prepared in example 1 can increase the biomass of volatile aroma substances in agilawood, and further improve the quality of the agilawood essential oil.
Table 1 detection results of each detection index of agilawood obtained in example 1
Table 2 detection results of indexes of the natural agilawood and the agilawood essential oil prepared from the agilawood obtained in example 1
The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge of those skilled in the art without departing from the gist of the present invention. Furthermore, the embodiments of the present invention and the features of the embodiments may be combined with each other without conflict.
Claims (6)
1. The agilawood formation accelerant is characterized by comprising the following components: agilawood water-soluble extract, lactobacillus plantarum bacterial liquid, saccharides and organic acid;
the agilawood edgeworthia chrysantha promoter comprises the following components in percentage by weight: 5 to 30 percent of agilawood water-soluble extract, 5 to 15 percent of lactobacillus plantarum bacterial liquid, 2 to 8 percent of saccharides and 0.5 to 3 percent of organic acid;
the preparation method of the agilawood water-soluble extract comprises the following steps: mixing the essential oil-extracted and sun-dried agilawood with water, and leaching to obtain the agilawood water-soluble extract.
2. The agilawood edgeworthia chrysantha promoter according to claim 1, wherein the sugar is one or more of white granulated sugar, glucose and sucrose.
3. The agilawood edgeworthia chrysantha promoter according to claim 1 or 2, wherein the organic acid is one or more of citric acid, tartaric acid, acetic acid, succinic acid and oxalic acid.
4. The preparation method of the agilawood edgeworthia chrysantha promoter according to any one of claims 1 to 3, characterized by comprising the following steps: mixing the agilawood water-soluble extract, saccharides, lactobacillus plantarum bacterial liquid, organic acid and water to obtain the agilawood knot fragrance accelerant.
5. Use of the agilawood edgeworthia chrysantha promoter according to any one of claims 1 to 3 in agilawood edgeworthia chrysantha.
6. A method for producing agilawood, characterized in that the agilawood production promoter according to any one of claims 1 to 3 is added to an aquilaria plant.
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