CN114847468A - Bamboo salt composition and application thereof - Google Patents
Bamboo salt composition and application thereof Download PDFInfo
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- CN114847468A CN114847468A CN202111364030.7A CN202111364030A CN114847468A CN 114847468 A CN114847468 A CN 114847468A CN 202111364030 A CN202111364030 A CN 202111364030A CN 114847468 A CN114847468 A CN 114847468A
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- 235000015334 Phyllostachys viridis Nutrition 0.000 title claims abstract description 84
- 239000011425 bamboo Substances 0.000 title claims abstract description 84
- 150000003839 salts Chemical class 0.000 title claims abstract description 78
- 239000000203 mixture Substances 0.000 title claims abstract description 39
- 235000002639 sodium chloride Nutrition 0.000 claims abstract description 63
- 239000000843 powder Substances 0.000 claims abstract description 52
- 235000003145 Hippophae rhamnoides Nutrition 0.000 claims abstract description 18
- 239000001506 calcium phosphate Substances 0.000 claims abstract description 17
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims abstract description 17
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- 229940078499 tricalcium phosphate Drugs 0.000 claims abstract description 17
- 229910000391 tricalcium phosphate Inorganic materials 0.000 claims abstract description 17
- 241000220317 Rosa Species 0.000 claims abstract description 16
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- 239000011647 vitamin D3 Substances 0.000 claims abstract description 16
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- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000011575 calcium Substances 0.000 claims abstract description 14
- 229910052791 calcium Inorganic materials 0.000 claims abstract description 14
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 claims abstract description 13
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000000811 xylitol Substances 0.000 claims abstract description 13
- 235000010447 xylitol Nutrition 0.000 claims abstract description 13
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 claims abstract description 13
- 229960002675 xylitol Drugs 0.000 claims abstract description 13
- 241000186604 Lactobacillus reuteri Species 0.000 claims abstract description 12
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- 240000000950 Hippophae rhamnoides Species 0.000 claims abstract 4
- 239000008267 milk Substances 0.000 claims description 11
- 210000004080 milk Anatomy 0.000 claims description 11
- 235000013336 milk Nutrition 0.000 claims description 11
- 239000000243 solution Substances 0.000 claims description 11
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Chemical compound [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 5
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- 235000013361 beverage Nutrition 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- 235000001465 calcium Nutrition 0.000 claims description 3
- 229910000027 potassium carbonate Inorganic materials 0.000 claims description 3
- 240000002547 Rosa roxburghii Species 0.000 claims description 2
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- 239000001103 potassium chloride Substances 0.000 claims description 2
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- 239000012086 standard solution Substances 0.000 claims description 2
- 230000000968 intestinal effect Effects 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 4
- 230000001737 promoting effect Effects 0.000 abstract description 3
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- 238000010521 absorption reaction Methods 0.000 abstract 1
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- 229910052500 inorganic mineral Inorganic materials 0.000 description 4
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- 241000894006 Bacteria Species 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 3
- 235000011613 Pinus brutia Nutrition 0.000 description 3
- 241000018646 Pinus brutia Species 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
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Images
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/40—Table salts; Dietetic salt substitutes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/385—Concentrates of non-alcoholic beverages
- A23L2/39—Dry compositions
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/15—Vitamins
- A23L33/155—Vitamins A or D
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/173—Reuteri
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/90—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in food processing or handling, e.g. food conservation
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- Molecular Biology (AREA)
- Inorganic Chemistry (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention provides a bamboo salt composition and application thereof, wherein the bamboo salt composition comprises specific contents of bamboo salt, xylitol, sea buckthorn powder, roxburgh rose powder, tricalcium phosphate, phyllostachys pubescens leaf powder, vitamin D3 and lactobacillus reuteri, and can promote calcium absorption and achieve the technical effect of promoting and regulating intestinal flora.
Description
Technical Field
The invention relates to a bamboo salt composition and a preparation method and application thereof.
Background
The traditional bamboo salt is extracted by putting sun salt into a three-year-old phyllostachys pubescens, sealing two ends with natural loess, and calcining pine trees as fuel at high temperature. The traditional bamboo salt can not achieve the effect of promoting the regulation of intestinal flora.
Disclosure of Invention
Based on the above, there is a need for a bamboo salt composition with the function of promoting intestinal flora regulation and application thereof.
The invention adopts the following technical scheme:
the invention provides a bamboo salt composition, which at least comprises nine-roasting bamboo salt, lactobacillus reuteri, tricalcium phosphate, sea buckthorn powder and roxburgh rose powder.
Preferably, the weight ratio of the nine-baked bamboo salt, the tricalcium phosphate, the sea buckthorn powder and the roxburgh rose powder is (80-99): 0.5-5: (0.5-5).
In some of these embodiments, the bamboo salt composition further comprises at least one of milk calcium, vitamin D3, phyllostachys edulis powder, and xylitol.
In some embodiments, the weight ratio of the nine-baked bamboo salt, the milk calcium, the vitamin D3, the phyllostachys edulis powder and the xylitol is (80-99): 0.3-3): 0.00001-0.0001): 0.5-5.
In some embodiments, the weight ratio of the nine-baked bamboo salt, the tricalcium phosphate, the sea buckthorn powder, the roxburgh rose powder, the milk calcium, the vitamin D3, the phyllostachys edulis leaf powder and the xylitol is (90-99): 0.5-2): 0.5-1.5): 0.3-3): 0.00001-0.0001): 0.5-1.0): 0.8-1.2.
In some of these embodiments, the Lactobacillus reuteri NCIMB30242 is added in an amount of 500 ten thousand to 10 hundred million CFU/g.
In some of these embodiments, the bamboo salt composition further comprises potassium carbonate and/or potassium chloride.
Use of a bamboo salt composition as described in any of the above for the preparation of a product for gut flora modulation.
A bamboo salt solid beverage comprises a bamboo salt composition containing any one of the above.
The invention also provides a bamboo salt and bamboo salt standard solution, which is a solution containing any one of the bamboo salt composition.
The invention has the beneficial effects that:
compared with the prior art, the bamboo salt composition has the advantages that the bamboo salt, the tricalcium phosphate, the sea buckthorn powder, the roxburgh rose powder, the milk calcium, the vitamin D3, the phyllostachys pubescens leaf powder and the xylitol are compounded to supplement calcium, mineral substances and trace elements needed by a human body, provide an alkaline environment close to body fluid, can be used as a solid beverage for regulating the balance of intestinal flora, reduce the problems caused by the lack of vitamin mineral substances, and expel toxin and diminish inflammation.
Drawings
Fig. 1 is a statistical chart of the application effect of the bamboo salt composition and the iodine salt of example 6.
Fig. 2 is a statistical chart of the application effect of the bamboo salt composition of example 6 and pure nine-bake bamboo salt.
Detailed Description
The present invention is further described in detail below with reference to specific examples so that those skilled in the art can more clearly understand the present invention.
The following examples are provided only for illustrating the present invention and are not intended to limit the scope of the present invention. All other embodiments obtained by a person skilled in the art based on the specific embodiments of the present invention without any inventive step are within the scope of the present invention.
In the examples of the present invention, all the raw material components are commercially available products well known to those skilled in the art, unless otherwise specified; in the examples of the present invention, unless otherwise specified, all technical means used are conventional means well known to those skilled in the art.
Description of key material sources:
the preparation process of the nine-roasting bamboo salt comprises the following steps: selecting natural sea salt, selecting fresh phyllostachys pubescens which grow for three years, cutting the phyllostachys pubescens into bamboo tubes with one section and one section, pressing the sea salt into a bamboo slip, sealing the bamboo slip with clean loess and mud with the depth of four meters underground, placing the sealed bamboo slip into a refining furnace, roasting the bamboo slip for 8-10 h at the temperature of about 800 ℃ by taking pine resin as fuel, burning the bamboo slip into ash to obtain a gray salt rod with mineral elements in the bamboo, loess and pine fused, and crushing the gray salt rod to obtain the roasted bamboo salt. And (4) putting the first-baked bamboo salt into a new bamboo tube again, and repeatedly baking to obtain second-baked bamboo salt. Placing the two-baked bamboo salt in a new bamboo tube again, and repeatedly baking for 9 times to obtain nine-baked bamboo salt.
Sea buckthorn powder, purchased from west ampere osier.
Rosa roxburghii powder, purchased from Sai' an Ousae.
Milk calcium, purchased from west anao.
Phyllostachys edulis powder purchased from west anao.
Example 1
The embodiment provides a bamboo salt composition, which is prepared by mixing the following raw materials: 95.7 wt% of nine-roasted bamboo salt, 0.8 wt% of tricalcium phosphate, 1 wt% of sea buckthorn powder, 1 wt% of roxburgh rose powder, 1 wt% of xylitol, 0.5 wt% of lophatherum gracile powder, 0.00005% of vitamin D3 and 10 hundred million CFU/g of lactobacillus reuteri NCIMB 30242.
Example 2
The embodiment provides a bamboo salt composition, which is prepared by mixing the following raw materials: 89.5 wt% of nine-roasted bamboo salt, 2 wt% of tricalcium phosphate, 3 wt% of sea buckthorn powder, 2 wt% of roxburgh rose powder, 0.5 wt% of xylitol, 3 wt% of lophatherum gracile powder, 0.00005% of vitamin D3 and 10 hundred million CFU/g of lactobacillus reuteri NCIMB 30242.
Example 3
The embodiment provides a bamboo salt composition, which is prepared by mixing the following raw materials: 89.5 wt% of nine-roasted bamboo salt, 2 wt% of tricalcium phosphate, 3 wt% of sea buckthorn powder, 2 wt% of roxburgh rose powder, 0.5 wt% of xylitol, 3 wt% of lophatherum gracile, 0.00005% of vitamin D3 and 10 hundred million/g of lactobacillus reuteri NCIMB 30242.
Example 4
The embodiment provides a bamboo salt composition, which is prepared by mixing the following raw materials: 88 wt% of nine-baked bamboo salt, 3.5 wt% of tricalcium phosphate, 0.5 wt% of milk calcium, 4 wt% of sea buckthorn powder, 0.5 wt% of roxburgh rose powder, 0.5 wt% of xylitol, 2 wt% of lophatherum gracile powder, 1 wt% of potassium carbonate, 0.00005% of vitamin D3 and 10 hundred million/g of lactobacillus reuteri NCIMB 30242.
Example 5
The embodiment provides a bamboo salt composition, which is prepared by mixing the following raw materials: 95 wt% of nine-roasted bamboo salt, 1 wt% of tricalcium phosphate, 2 wt% of sea buckthorn powder, 2 wt% of roxburgh rose powder, 0.00005% of vitamin D3 and 10 hundred million/g of lactobacillus reuteri NCIMB 30242.
Example 6
The embodiment provides a bamboo salt composition, which is prepared by mixing the following raw materials: 94.5 wt% of nine-baked bamboo salt, 1 wt% of tricalcium phosphate, 2 wt% of sea buckthorn powder, 2 wt% of roxburgh rose powder, 0.5 wt% of lophatherum gracile powder, 0.00005% of vitamin D3 and 10 hundred million/g of lactobacillus reuteri NCIMB 30242.
Example 7
The embodiment provides a bamboo salt composition, which is prepared by mixing the following raw materials: 94.5 wt% of nine-baked bamboo salt, 1 wt% of tricalcium phosphate, 2 wt% of milk calcium, 2 wt% of sea buckthorn powder, 2 wt% of roxburgh rose powder, 0.5 wt% of lophatherum gracile powder, 0.00005% of vitamin D3 and 10 hundred million/g of lactobacillus reuteri NCIMB 30242.
Comparative example 1
This comparative example provides a bamboo salt composition having substantially the same composition as example 1, except that: the sea buckthorn powder and the tricalcium phosphate are replaced by nine kinds of baked bamboo salt with the same amount.
Comparative example 2
This comparative example provides a bamboo salt composition having substantially the same composition as example 6, except that: equal amount of nine-roasting bamboo salt is used to replace light bamboo leaf powder and roxburgh rose powder.
The bamboo salt composition 2 prepared in examples 1, 6 and 7 and comparative examples 1 and 2 were used to prepare physiological salt solution, and the preparation method comprises the following steps:
physiological concentration salt solution: 0.9g of the salt was weighed, dissolved in ultrapure water, and diluted to 100 mL.
2-fold physiological concentration saline solution: 1.8g of the salt was weighed, dissolved in ultrapure water, and diluted to 100 mL.
The efficacy of the bamboo salt physiological solution prepared in the examples 1, 6 and 7 and the comparative examples 1 and 2 is respectively researched by taking commercially available iodine salt and nine-roasted bamboo salt as a control, and the method comprises the following steps:
(1) preparing a culture medium:
60g of the improved GAM broth is weighed by an electronic analytical balance and recorded, the improved GAM broth is poured into a 1000mL beaker, 800mL of ultrapure water is weighed by a measuring cylinder and poured into the beaker, a stirring rotor is placed, the beaker is placed on a magnetic heating stirrer and stirred until the improved GAM broth is completely dissolved, a glass rod is used for draining the improved GAM broth into a 1000mL volumetric flask, a small amount of ultrapure water is used for flushing the beaker, the volumetric flask is poured, and the process is repeated for 3 times. The liquid in the volumetric flask is fixed to the scale mark by ultrapure water, and the liquid is turned upside down for 3 times after the cap is plugged, so that the solution is ensured to be fully mixed.
The solution after constant volume is poured into a clean yellow glass bottle with a cover, 500mL of culture medium is put in each 1000mL bottle, and the solution is prevented from being sprayed out during autoclaving. The name, volume, date, effective period, operator and other information of the culture medium are marked on the bottle body.
And (3) sterilization: the glass bottle with the yellow cap containing the culture medium is unscrewed and placed into an autoclave for 15 minutes at 121 ℃. After sterilization, the bottle cap is immediately screwed down and cooled to room temperature.
(2) Pretreatment of sample solutions
The total mass of the stool sample was weighed out on a hundredth scale. An empty 50mL centrifuge tube is first placed on a balance and zeroed before the sample is weighed 10 g. An appropriate amount of PBS buffer was taken by an automatic pipette and added to the centrifuge tube and mixed well on a shaker. And (3) evenly distributing the completely mixed sample into new 50ml centrifuge tubes on average, and adding a proper amount of PBS buffer solution into each tube. Filtering in a biological safety cabinet after uniformly mixing, and sequentially passing the diluted samples through 20-mesh, 50-mesh, 100-mesh and 200-mesh filter screens. Placing the centrifuge tube into a centrifuge, balancing, centrifuging at 6000G and 4 deg.C for 15min, and discarding the supernatant. After weighing the precipitate, the volume was adjusted to 5% of the final concentration. The aliquots were immediately and evenly dispensed into new 50mL centrifuge tubes as required by the experiment. And (4) immediately feeding the subpackaged sample diluent into an anaerobic incubator.
After filtration, 0.5mL of 50% glycerol was added to 2mL of the cryopreserved tube, followed by 0.5mL of the viable bacteria sample. 6 tubes are backed up and put into a refrigerator at minus 80 ℃ for storage.
(3) salt-GAM full culture medium configuration
The blank glass culture dish side is correspondingly coded. The medium was dispensed into glass tubes 2mL per tube using a large volume pipette. The cap was placed over the finish and masked. Find the corresponding numbered glass bottle and physiological saline solution, open the cover. The salt solution was removed with a 1mL pipette and transferred to correspondingly numbered glass petri dishes, 2mL per tube. Attention is paid to replacing the gun head to prevent cross contamination. Finally the lid is screwed down.
(4) Experimental grouping (3 replicates per group) and anaerobic culture operation:
experimental group 2mL of culture medium, 2mL of bamboo salt composition solution and 1mL of sample bacteria solution
The condition control group comprises 2mL culture medium, 2mL iodine salt/nine-roast bamboo salt solution and 1mL PBS buffer solution
Blank control group 2mL Medium +3mL PBS buffer
Determining a power main power key on a control panel of the anaerobic incubator, electrifying the equipment, pressing a works space key on the side surface of the anaerobic incubator to replace the gas environment in the incubator for four hours, and then setting the anaerobic incubator in an anaerobic state; after confirming that each glass tube cover is screwed, transferring the glass tube cover into an anaerobic box transfer box, and disinfecting with a disinfectant before placing. When the diluted fecal sample bacteria are placed into the transfer chamber, the outer surface is sprayed with disinfectant, the outer door of the transfer chamber is opened, the transfer chamber is rapidly placed into the anaerobic box transfer chamber, the outer door is closed, and the auto cycle key is pressed, so that the transfer chamber is in an anaerobic state; after placing in the anaerobic chamber, the caps were unscrewed and O2 was replaced overnight.
The fecal specimen bacterial solution was transferred to the numbered salt-GAM whole medium and blank groups with 1mL pipette, 1mL per tube. After the sample is applied, the cap is unscrewed. After 72 hours of culture observation, culture products are collected, and sent for detection, and the detection statistics of the flora abundance are obtained, and the results are shown in the following tables 1 to 3 and figures 1 and 2:
table 1 statistics for differences in abundance of Succinivibrio flora
Table 2 statistical results for differences in the abundance of Bacteroides flora
Table 3 statistics for differences in abundance of Prevotella flora
Actually, the bamboo salt composition disclosed by the invention can supplement calcium, mineral substances and trace elements required by a human body by preferably compounding bamboo salt, tricalcium phosphate, sea buckthorn powder, roxburgh rose powder, milk calcium, vitamin D3, lophatherum gracile powder and xylitol, provides an alkaline environment close to body fluid, can be used as a solid beverage for regulating the balance of intestinal flora, reduces the problems caused by the deficiency of vitamin and mineral substances, and is capable of expelling toxins and diminishing inflammation.
It should be noted that the above examples are only for further illustration and description of the technical solution of the present invention, and are not intended to further limit the technical solution of the present invention, and the method of the present invention is only a preferred embodiment, and is not intended to limit the protection scope of the present invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A bamboo salt composition is characterized by at least comprising nine-roasted bamboo salt, Lactobacillus reuteri, tricalcium phosphate, sea buckthorn powder and roxburgh rose powder.
2. The bamboo salt composition as claimed in claim 1, wherein the weight ratio of the nine-roasted bamboo salt, the tricalcium phosphate, the sea buckthorn powder and the roxburgh rose powder is (80-99): 0.5-5: (0.5-5).
3. The bamboo salt composition as claimed in claim 2, further comprising at least one of milk calcium, vitamin D3, phyllostachys edulis powder and xylitol.
4. The bamboo salt composition as claimed in claim 3, wherein the weight ratio of the nine-roasted bamboo salt, the milk calcium, the vitamin D3, the phyllostachys edulis powder and the xylitol is (80-99): (0.3-3): 0.00001-0.0001): (0.5-5).
5. The bamboo salt composition as claimed in claim 4, wherein the weight ratio of the nine-roasted bamboo salt, tricalcium phosphate, sea buckthorn powder, rosa roxburghii powder, milk calcium, vitamin D3, phyllostachys pubescens leaf powder and xylitol is (90-99): 0.5-2): 0.5-1.5): 0.3-3): 0.00001-0.0001): 0.5-1.0): 0.8-1.2.
6. Bamboo salt composition according to claim 5, wherein the Lactobacillus reuteri NCIMB30242 is added in an amount of 500 ten thousand to 10 hundred million CFU/g.
7. Bamboo salt composition according to any one of claims 1 to 6, further comprising potassium carbonate and/or potassium chloride.
8. Use of a bamboo salt composition according to any one of claims 1 to 7 for the preparation of a gut flora modulating product.
9. A bamboo salt solid beverage characterized by comprising the bamboo salt composition of any one of claims 1 to 7.
10. A bamboo salt standard solution characterized by being a solution containing the bamboo salt composition according to any one of claims 1 to 7.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20010066711A (en) * | 1999-12-31 | 2001-07-11 | 김원배 | Bamboo Salt Using Veinstone |
| CN106509913A (en) * | 2016-10-25 | 2017-03-22 | 天益健康科学研究院（镇江）有限公司 | Composition for regulating balance of intestinal flora |
| CN107114723A (en) * | 2017-05-05 | 2017-09-01 | 广州赛莱拉干细胞科技股份有限公司 | It is a kind of to improve the composition and a kind of fruit juice of improvement enteric microorganism of enteric microorganism |
| CN110800985A (en) * | 2019-11-25 | 2020-02-18 | 武汉绿时代创新科技有限公司 | Multi-variety edible bamboo salt fused with sea buckthorn and preparation method thereof |
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2021
- 2021-11-17 CN CN202111364030.7A patent/CN114847468A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20010066711A (en) * | 1999-12-31 | 2001-07-11 | 김원배 | Bamboo Salt Using Veinstone |
| CN106509913A (en) * | 2016-10-25 | 2017-03-22 | 天益健康科学研究院（镇江）有限公司 | Composition for regulating balance of intestinal flora |
| CN107114723A (en) * | 2017-05-05 | 2017-09-01 | 广州赛莱拉干细胞科技股份有限公司 | It is a kind of to improve the composition and a kind of fruit juice of improvement enteric microorganism of enteric microorganism |
| CN110800985A (en) * | 2019-11-25 | 2020-02-18 | 武汉绿时代创新科技有限公司 | Multi-variety edible bamboo salt fused with sea buckthorn and preparation method thereof |
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