CN114835778B - 一种h9n2亚型aiv mhc b2限制性表位肽及其应用 - Google Patents
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Abstract
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CN202210449829.4A CN114835778B (zh) | 2022-04-27 | 2022-04-27 | 一种h9n2亚型aiv mhc b2限制性表位肽及其应用 |
US18/305,735 US20230346912A1 (en) | 2022-04-27 | 2023-04-24 | Restrictive epitope peptide of major histocompatibility complex b2 of h9n2 subtype avian influenza virus and application thereof |
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CN202210449829.4A CN114835778B (zh) | 2022-04-27 | 2022-04-27 | 一种h9n2亚型aiv mhc b2限制性表位肽及其应用 |
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CN114835778A CN114835778A (zh) | 2022-08-02 |
CN114835778B true CN114835778B (zh) | 2023-06-06 |
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Title |
---|
hypothetical protein, partial [Arthrobacter psychrolactophilus];NCBI Reference Sequence: WP_146233446.1;unknown;《NCBI》;全文 * |
Identification and characterization of a novel antigenic epitope in the hemagglutinin of the escape mutants of H9N2 avian influenza viruses;Yinbiao Zhu等;《Veterinary Microbiology》;第178卷(第1-2期);第144-149页 * |
通用H9N2禽流感病毒抗原表位的筛选;黄锐坤等;《中国优秀硕士学位论文全文数据库(电子期刊);A006-84 * |
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