CN114806849A

CN114806849A - Microbial purification appearance

Info

Publication number: CN114806849A
Application number: CN202210606803.6A
Authority: CN
Inventors: 褚殿慧; 郑富全; 狄增如; 吴思; 庞寓天
Original assignee: Ruishi Intelligent Jinan Technology Research Center LP
Current assignee: Ruishi Intelligent Jinan Technology Research Center LP
Priority date: 2022-05-31
Filing date: 2022-05-31
Publication date: 2022-07-29

Abstract

The invention relates to a microorganism purifying instrument. The processing system for automatically purifying the microorganisms can realize automatic identification and seed taking, the camera shoots images during seed taking, the images are analyzed by the computer, and microbial colonies with higher purity can be found after the images are processed by the computer algorithm. Calculate bacterial colony position and issue the instruction and accomplish for the system and get kind of operation, solved the problem that current automatic seed-picking equipment can't realize bacterial colony discernment. In addition, the microbial purification instrument provided by the invention provides a reasonable culture dish moving line through the arrangement of the working table top, the storage table top and the lifting device, the input and the output of the culture dish are culture dishes, the problem of damage caused by the fact that the culture dish needs to be replaced and the culture dish falls freely in the existing equipment is solved, and the microbial purification instrument is a purification system which can automatically operate and has higher biological safety.

Description

Microbial purification appearance

Technical Field

The invention belongs to the technical field of microbial purification, and particularly relates to a microbial purification instrument.

Background

The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and is not necessarily to be construed as an admission or any form of suggestion that this information forms the prior art that is already known to a person of ordinary skill in the art.

The process of selecting one or a certain strain of microorganism from a heterogeneous population of microorganisms is called isolation and purification of the microorganism. In the research and application of molecular biology, not only separation and purification technology is required to separate a specific microorganism from a microorganism population, but also the purity of the microorganism must be maintained to prevent other biological contamination. Among them, the streaking method is a common method for microbial purification, in which microbes mixed together or different cells in the same microbial population are diluted by inoculating loops on the surface of a plate medium by zone streaking to obtain a plurality of single cells distributed independently, and the single cells are cultured and propagated to form a single colony, and the single colony is usually regarded as a pure species of a microbe to be separated. The prior common marking method mainly adopts manual marking or automatic marking, and the manual seed taking and marking purification mainly has the following problems:

(1) when the microorganism sample is manually marked, the microorganism sample is exposed in the environment, so that biological pollution is caused to the environment, and personal harm is caused to operators; meanwhile, the environment can also interfere with the culture of microorganisms, and the purification result is influenced.

(2) The manual operation has high requirements on the whole sterile environment, and complicated operation is required. The microorganism needs to be repeatedly selected and streaked during purification to reach proper purity, and the whole process is time-consuming and labor-consuming in manual operation and high in labor cost.

(3) The manual streaking operation has instability when streaking and smearing according to the hand feeling, and inevitably brings human errors, randomness and nonstandard operation to influence the final result.

Other automatic marking devices currently have the following problems:

(1) most automated microbiological processing systems sample mixed sample tubes. Lack of identification of microbial colonies, inability to automatically identify and select seeds, high mixing degree of new samples after streaking, and difficulty in purification.

(2) The input of the existing equipment is a test tube sample, and the output is a culture dish. The purification of the multiple scribing treatment cannot be achieved only by once scribing.

(3) When the culture dish is loaded, the culture dish is pushed from the lowest culture dish to be taken, and the upper culture dish freely falls to easily damage the culture dish. And partial ware all has a recess from top to bottom, and the mode of promoting to take can't solve this type of ware.

(4) The handwritten mark on the sample culture dish can not be identified, the coding information of the existing equipment is printed, and the handwriting of an operator can not be identified.

(5) The culture dish after marking has no mark or uses the mode of labeling, and no mark is easy to be confused to cause the sample information error, and the mechanical structure of the mode of labeling is complicated.

Disclosure of Invention

The invention provides a novel microorganism sample purification system, which realizes automatic feeding and automatic uncovering of a sample, identification of handwritten or printed mark information on the side and the bottom of a sample culture dish, automatic image analysis and identification of microorganism groups in the sample dish, automatic selection of sample microorganisms with high purity, determination of the positions of the sample microorganisms for seed taking, automatic uncovering of a clean culture dish, code spraying and marking of an inoculated culture dish according to sample information, streaking culture and recovery of the sample dish and the streaked culture dish. The contact between people and the environment and the microorganisms in the culture dish in the process of seed taking and streaking is avoided. Environmental pollution is avoided, and operators are easily injured. And the interference of the external environment to the culture dish is also reduced.

In a first aspect of the present invention, a microbial purification apparatus is provided, which includes a rack, a scribing module, a sample carrying switch cover module, a vision camera system, a sample storage lifting module, an inkjet printer, a culture dish carrying module, a seed taking identification rotating module, and a culture dish storage lifting module;

the rack is a square frame main body, the middle part of the rack is provided with a working table surface, and the bottom of the rack is provided with a storage table surface; the working table top is used for completing seed taking, lineation, inoculation and code spraying marking of the sample vessel, and the storage table top is used for completing storage, taking and placing of the sample vessel, the plate vessel and the culture vessel;

the sample carrying switch cover module and the vision camera system are positioned on the left side of the working table, and the sample storage lifting module is positioned in the left space of the working table;

the ink-jet printer and the culture dish carrying module are positioned on the right side of the working table, and the culture dish storage lifting module is positioned in the right space of the working table and the storage table;

the scribing module is located above the working table.

Preferably, the top of the microorganism purifier is provided with a control system, the control system is used for driving, and the control system can control and display data of the microorganism purifier through a liquid crystal display screen arranged at the top of the microorganism purifier.

Further preferably, the top of the microorganism purification instrument is also provided with an ultraviolet disinfection lamp, the ultraviolet disinfection lamp is positioned above the working table, and the working table is covered by illumination after the ultraviolet disinfection lamp is started.

Preferably, the working table and the frame are movably connected, and four corners of the working table are movably connected with the frame of the frame, so that the leveling of the working table or the height adjustment of the working table is facilitated.

Preferably, the left side of the working table surface is provided with three holes, the centers of the holes are collinear, and a sample vessel ascending channel, a sample photographing and seed taking table and a recovery vessel descending channel are sequentially arranged from left to right, wherein the sizes of the sample vessel ascending channel and the recovery vessel descending channel can accommodate a culture vessel to pass through; the sample carrying, cover opening and cover closing module is fixed on the working table surface, comprises two groups of slide rails and clamps fixed on the slide rails, and respectively comprises a sample bottom moving module, a sample clamp dish clamping jaw, a sample clamp cover clamping jaw and a sample cover moving module, wherein the sample bottom moving module and the sample cover moving module are a pair of slide rails arranged in parallel and are distributed on two sides of a sample dish lifting channel, a sample photographing and seed taking table and a recovery dish descending channel, and the sample clamp dish clamping jaw is fixed on the sample bottom moving module and can move along the sample bottom moving module; the sample cover clamping jaw is fixed on the sample cover moving module and can move along the sample cover moving module; there is the difference in height between sample clamp closure clamping jaw and the sample presss from both sides the ware jack catch.

Preferably, the table surface right side is opened has two holes that can hold the culture dish and pass through, for dish ascending channel and culture dish decline passageway, is marking off workstation and inkjet sign indicating number platform in one side of two hole center lines, the table surface right side still has culture dish transport module, including four slide rails and two grabs, is plate clamp cover clamping jaw, plate respectively and moves and cover the module, plate and move end module, plate clamp end clamping jaw, marking off group dish module and inkjet group dish module, plate clamp cover clamping jaw is installed on the slider that the plate moved the cover module, plate clamp end clamping jaw is installed on the slider that the plate moved end module.

Furthermore, the centers of the scribing worktable and the ink-jet coding table are positioned on the extension lines of the centers of three holes of the sample vessel ascending channel, the sample photographing and seed taking table and the recovery vessel descending channel; the center connecting lines of the scribing workbench, the ink-jet coding table, the plate ascending channel and the culture dish descending channel form a rectangle, and the plate cover moving module, the plate bottom clamping jaw, the scribing dish shifting module and the ink-jet dish shifting module are parallel to the rectangle and positioned on the outer side of the rectangle; the dish moves die block group and is located the dish ascending passageway and the streak inoculation platform outside, the dish moves lid module and is located the dish ascending passageway and the culture dish decline passageway outside, the dish module is dialled in the streak is located the streak revolving stage and the inkjet revolving stage outside to the streak, the dish module is dialled in the inkjet is located the inkjet revolving stage and the culture dish decline passageway outside to the inkjet.

Furthermore, an ink-jet printer is arranged beside the ink-jet printing table and used for carrying out ink-jet marking on the culture dish after lineation, and a nozzle of the ink-jet printer is tangent to the edge of the ink-jet rotating table.

Preferably, the scribing module is fixed on the rack and located above the workbench surface, the scribing module comprises X, Y, R-axis three mechanical arms, wherein an X axis is fixedly connected with the rack, a Y axis is connected with a sliding block arranged on the X axis and can slide along the X axis, an R axis is arranged on the sliding block of the Y axis and can slide along the Y axis, and a seed taking device is loaded on the R axis; the Y-axis sliding block is connected with the motor reducer of the R axis by a connecting piece and is used for controlling the seed taking device to be close to or far away from the working table surface.

Preferably, the visual camera system comprises a microorganism sample identification camera, a prism, a culture dish periphery code spraying identification camera, a plane mirror, a culture dish bottom mark identification camera and a camera fixing frame; the microorganism sample identification camera is fixed right above the sample photographing seed taking table, and light sources are uniformly distributed from the periphery of the camera to irradiate the sample vessel; the plane mirror is arranged right below the sample photographing and seed taking table, and the prism penetrates through the working table surface and is positioned between the sample photographing and seed taking table and the sample cover moving module; the culture dish periphery code-spraying recognition camera is fixed below the working table board, is at the same horizontal position with the prism, and is used for reading the culture dish periphery code-spraying; the culture dish bottom mark identification camera is also arranged below the working table surface and is at the same horizontal position with the plane mirror for reading the culture dish bottom mark.

Preferably, the sample storage, conveying, lifting and recovery module comprises a sample lifting module, a recovery lifting table, a recovery bin storage and conveying module, a sample lifting table and a sample bin storage and conveying module; the sample bin conveying module and the recycling bin storage conveying module are a pair of conveying belts, parallel grooves are formed in the storage table surface, and the sample bin conveying module and the recycling bin storage conveying module are installed through fixing pieces; the sample lifting module and the recovery lifting module are a pair of grooved guide rails, are arranged between the working table top and the storage table top and are vertically arranged; sample elevating platform, recovery elevating platform are used for fixed sample ware and recovery ware respectively, carry the module, retrieve storehouse storage through the slider and carry module, sample elevating module, retrieve the elevating module and move on for realize the transportation and the recovery of sample ware.

Further, the guide plates are distributed on two sides of the sample bin conveying module in parallel along the grooves, the dishes are prevented from being toppled left and right, and the guide plates are perpendicular to the storage table board.

Preferably, the culture dish lifting storage module comprises a plate lifting module, a plate storage device, a culture dish lifting module and a culture dish storage device; the plate lifting module comprises a lifting platform for clamping a plate and a guide rail for operating the lifting platform, is arranged between the storage table surface and the working table surface, is vertical to the working table surface, and can move between the storage table surface and the working table surface through the guide rail; the plate storage device is arranged on the storage plane, and a plurality of storage channels are distributed on the plate storage device and are uniformly distributed in a circumferential array; the culture dish lifting module is arranged between the storage table surface and the working table surface, is vertical to the working table surface, and comprises a lifting platform for clamping a culture dish and a guide rail for the operation of the lifting platform; the culture dish storage device is arranged on the storage plane, the storage channels are uniformly distributed in a circumferential array manner, and the lifting platform of the lifting module can move between the storage platform surface and the working platform surface.

Preferably, the microorganism purification instrument further comprises a seed taking device recovery bin, wherein the seed taking device recovery bin is fixed on the working table surface and is a tank body used for recovering and using the seed taking device.

In one embodiment of the above preferred embodiment, the operation of the microorganism purification apparatus is as follows: the sample vessel is stored on the sample bin storage and conveying module, the sample vessel is moved to the rear end of the sample bin storage and conveying module through the matching of the sample bin storage and conveying module and the sensor, the sample lifting table of the sample lifting module lifts the bottommost part of the sample vessel from the bottom and moves to the working table surface along the sample lifting channel, the sample vessel is opened through the sample carrying, cover opening and closing module, the sample vessel is moved to the sample photographing and seed taking position, the sample vessel clamping claw is loosened, and the sample vessel is placed on the seed taking identification rotating module; the seed taking and identifying rotary module rotates to enable the visual camera system to identify codes on the side surface and the bottom of the sample vessel, sample information on the front surface of the sample vessel enters the microbial sample identifying camera, the system automatically selects a microbial colony with higher purity according to the information, determines the position of the microbial colony, and extracts a microbial sample through the seed taking device of the scribing module; the sample vessel after the seed taking is changed into a recovery vessel, and the recovery vessel is moved to a recovery conveying module through a sample storage, conveying, lifting and recovery module; blank culture dish of plate storage device storage is moved to table surface and is accomplished uncapping by culture dish lift storage module, accomplishes the inoculation at the streak inoculation platform, later removes to the inkjet coding platform and carries out the coding, retrieves to storing the mesa by culture dish decline passageway after the coding is accomplished.

The beneficial effects of one or more technical schemes are as follows:

1. the invention can put a plurality of batches of different samples into the equipment and can identify the information of the handwritten and printed samples. The problem of current equipment can't discern the handwriting mark is solved.

2. The invention can realize automatic identification and seed taking, the camera shoots images during seed taking, the computer analyzes the images, and microbial colonies with higher purity can be found after the images are processed by the computer algorithm. And calculating the position of the bacterial colony, and issuing an instruction to the system to finish the seed taking operation. Solves the problem that the bacterial colony identification is not available in seed taking.

3. The input of the invention is a sample vessel, the output is a culture vessel, the output can be put into equipment after culture to continue to take seeds, line and purify, and the problem that the vessel needs to be replaced due to the non-uniform input and output of other equipment is solved.

4. The feeding mode of the invention is from bottom to top. The dish cover and the dish bottom are clamped separately. The lifting module and the carrying module are matched to carry out loading and unloading, and a fall exists when the loading is avoided through accurate electromechanical control. The problem of other equipment can't handle the culture dish that the ware body has the recess is solved. The problem of other modes have the drop, and the damage of culture dish free fall to self is solved.

5. The coding mode of the invention is as follows: and ink jetting and code endowing are carried out, and the code is sprayed on the scribed sample in real time, so that the scribed sample is prevented from being confused. Compared with the labeling mode, the space of the labeling machine is saved.

6. The invention realizes full automation of the microorganism purification process. The contact between people and microorganisms in the manual seed taking and scribing process is avoided, and harm to people and environmental pollution are prevented. The interference of the external environment to the purification process is reduced. The invention can complete manual marking action, and also can realize spiral marking, which is more uniform than the traditional manual marking mode. The microorganism is uniformly distributed, the purification efficiency is improved, and the purification time is shortened.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this specification, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate exemplary embodiments of the invention and together with the description serve to explain the invention and not to limit the invention.

FIG. 1 is a schematic isometric view of the structure of the present invention;

FIG. 2 is a schematic elevational view of the structure of the present invention;

FIG. 3 is a schematic view of the position of the plate handling module according to the present invention;

FIG. 4 is a schematic view of the storage structure and loading/unloading of the culture dish of the present invention;

FIG. 5 is an optical diagram of the vision camera system [4] of the present invention;

FIG. 6 is a schematic view of a seed-picking identification rotation module according to the present invention

Wherein, 1-the frame, 1.1-the liquid crystal touch screen, 1.2-the ultraviolet disinfection lamp, 1.3-the working table, 1.4-the storage table; 2-a scribing module; 3-sample carrying, cover opening and closing module, 3.1-sample bottom moving module, 3.2-sample clamp dish clamping jaw, 3.3-sample clamp cover clamping jaw and 3.4-sample cover moving module; 4-vision camera system, 4.1-microorganism sample identification camera, 4.2-prism, 4.3-culture dish periphery code spraying identification camera, 4.4-plane mirror, 4.5-culture dish bottom mark identification camera and 4.6-camera fixing frame; 5-recovering bin of waste seed-taking device; 6-a sample storage, conveying, lifting and recovering module and a 6.1 sample lifting module; 6.2-recovering the lifting module; 6.3-recovery lifting platform, 6.4-recovery bin storage and conveying module, 6.5-sample lifting platform and 6.6-sample bin storage and conveying module; 7-an ink jet printer; 8-culture dish carrying, 8.1-dish clamping cover clamping jaw, 8.2-dish cover moving module, 8.3-dish bottom moving module, 8.4-dish clamping bottom clamping jaw, 8.5-scribing dish shifting module and 8.6-ink-jet dish shifting module; 9-seed taking identification rotating module, 9.1-turntable motor, 9.2-motor fixing seat, 9.3-driving wheel, 9.4-turntable fixing seat, 9.5-turntable and 9.6-driven wheel; 10-culture dish lifting storage module, 10.1-plate lifting module, 10.2-plate storage device and 10.3-culture dish lifting module 10.4-culture dish storage device; 11-sample vessel ascent channel; 12-taking a seed position by photographing a sample; 13-recovery dish descending channel; 14-plate ascending channel; 15-streaking inoculation station; 16-an inkjet numbering station; 17-Petri dish Down channel.

Detailed Description

It is to be understood that the following detailed description is exemplary and is intended to provide further explanation of the invention as claimed. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

In the following description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", "lateral", "vertical", and the like, are used in the orientations and positional relationships indicated in the drawings, which are based on the orientations and positional relationships shown in the drawings, and are used for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.

In the present invention, unless otherwise explicitly specified or limited, the terms "connected," "fixed," and the like are to be construed broadly, and for example, "fixed" may be a fixed connection, a detachable connection, or an integral part; may be a mechanical connection, may be an electrical connection; either directly or through an intermediary, either internally or in any other relationship, unless expressly specified otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

Interpretation of terms:

plating: the term "plate" in the present document denotes a blank culture dish without inoculated strain.

Sample vessel: the term "sample dish" in the present document refers to a microorganism culture dish before purification.

In order to make the technical solutions of the present invention more clearly understood by those skilled in the art, the technical solutions of the present invention will be described in detail below with reference to specific embodiments.

Example 1

In the embodiment, a microbial purification appearance that possesses automatic feeding, culture dish uncap, vision identification seed selection, gets kind of, streak culture, culture dish according to the image and retrieve the function is provided, microbial purification appearance includes frame [1], streak module [2], sample handling switch lid module [3], vision camera system [4], get kind of a ware and retrieve storehouse [5], lifting module [6] is stored to the sample, ink jet printer [7], culture dish transport module [8], get kind of a discernment rotation module [9], lifting module [10] is stored to the culture dish.

The frame [1] is a frame main body of the microorganism purifying instrument, the top of the frame [1] is provided with a liquid crystal touch screen [1.1] and an ultraviolet disinfection lamp [1.2], the middle part of the frame [1] is fixed with a working table surface [1.3], and the bottom part is fixed with a storage table surface [1.4 ]. The ultraviolet disinfection lamp [1.2] can be illuminated after being turned on to cover the working table surface [1.3], the working table surface [1.3] is arranged in parallel with the storage table surface [1.4], and four corners of the working table surface [1.3] can move on a frame of the machine frame [1] to realize leveling of the working table surface [1.3] and adjustment of the height of the working table surface [1.3 ].

The left side of the working table surface [1.3] is provided with 3 holes in a collinear way, and the sample vessel ascending channel [11], the sample photographing and seed taking platform [12] and the recovery vessel descending channel [13] are sequentially arranged, wherein the sample vessel ascending channel [11] and the recovery vessel descending channel [13] can accommodate culture vessels to pass through. The sample carrying, opening and closing module [3] is fixed on the working table surface [1.3] and comprises two groups of slide rails and clamping clips fixed on the slide rails, namely a sample bottom moving module [3.1], a sample clamp dish clamping jaw [3.2], a sample clamping cover clamping jaw [3.3] and a sample cover moving module [3.4 ]; wherein, the sample bottom moving module [3.1] and the sample cover moving module [3.4] are a pair of slide rails arranged in parallel and distributed at two sides of the sample vessel ascending channel [11], the sample photographing and seed taking platform [12] and the recovery vessel descending channel [13 ]. The sample clamping cover clamping jaw [3.3] is fixed on the sample moving bottom die set [3.1] and moves along the sample moving bottom die set [3.1] and is used for grabbing the bottom of a culture dish and moving the culture dish, and the sample clamping cover clamping jaw [3.3] is fixed on the sample moving cover module [3.4] and can move along the sample moving cover module [3.4] and is used for grabbing a culture dish cover and moving the culture dish cover. The height difference exists between the clamping cover clamping jaw (3.3) and the dish clamping jaw (3.2), so that collision interference in mutual movement is prevented. The contents of the work mainly completed on the left side of the working table surface [1.3] are as follows: the sample vessel arrives at the workbench from the sample vessel ascending channel [11], the sample vessel is controlled to move and open the cover through the sample carrying and cover opening and closing module [3], the works such as photographing and seed taking are completed, and the sample vessel descends to the storage table surface [1.4] through the recovery vessel descending channel [13 ].

Two holes for accommodating culture dishes to pass through are formed in the right side of the working table surface [1.3], a plate ascending channel [14] and a culture dish descending channel [17], a scribing working table [15] and an ink-jet coding table [16] are distributed in parallel on one side of a hole line (a central connecting line of the plate ascending channel [14] and the culture dish descending channel [17 ]), and an ink-jet printer [7] is arranged beside the ink-jet coding table [16] and used for marking the scribed culture dishes. The right side of the working table surface [1.3] is also provided with a culture dish carrying [8] module, and a dish clamping cover clamping jaw [8.1] is arranged on a slide block of the dish cover moving module [8.2 ]. The plate moving bottom die group [8.3] is distributed in parallel with the line (the connecting line of the plate lifting channel [14] and the scribing rotary table [15 ]) and is positioned at the left side of the line. The plate bottom clamping jaw [8.4] is arranged on a slide block of the plate bottom moving die set [8.3 ]. The scribing and dish-poking modules [8.5] are distributed in parallel with the line (the connecting line of the scribing rotary table [15] and the ink-jet rotary table [16 ]) and are positioned above the line. The ink-jet dish-poking module [8.6] is parallel to a line (a connecting line of the ink-jet rotating platform [16] and the culture dish descending channel [17 ]) and is positioned on the right side of the ink-jet dish-poking module, and optimally, a spray head is tangent to the edge of the ink-jet rotating platform.

The scribing module [2] is fixed on the rack [1] and arranged above the working table surface [1.3] and keeps a certain working distance. The scribing module [2] consists of three X/Y/R axes, wherein the X-axis module is fixedly connected with the rack [1 ]. The X-axis sliding block is connected with the Y-axis module, the Y-axis sliding block is connected with a motor speed reducer of the R-axis through a connecting piece, and the R-axis drives the seed taking device loading device to rotate.

The vision camera system [4] comprises a microorganism sample identification camera [4.1], a prism [4.2], a culture dish periphery code spraying identification camera [4.3], a plane mirror [4.4], a culture dish bottom mark identification camera [4.5] and a camera fixing frame [4.6 ]. Wherein, the microorganism sample recognition camera [4.1] is fixed right above the sample photographing seed taking table [12], and light sources are uniformly distributed around the camera to irradiate the sample. The plane mirror [4.4] is arranged right below the sample photographing and seed taking table [12], and the prism [4.2] penetrates through the working table surface [3.1] and is positioned between the sample photographing and seed taking table [12] and the sample cover moving module [3.4 ]. The culture dish periphery code-spraying recognition camera [4.3] is fixed below the working table surface [3.1] and is at the same horizontal position with the prism [4.2] for reading the culture dish periphery code-spraying. The culture dish bottom mark recognition camera [4.5] is also arranged below the worktable surface [3.1] and is at the same horizontal position with the plane mirror [4.4] for reading the culture dish bottom mark. The prism [4.2] is arranged by utilizing the principle of a periscope, coded image information on the desktop is reflected to the lower part of the desktop to be read, and the plane mirror also reflects the marked information at the bottom of the dish to the lens.

The sample storage, conveying, lifting and recovery module is positioned at the left part of the storage table surface [1.4], the sample bin conveying module [6.6] and the recovery bin storage and conveying module [6.4] are a pair of conveying belts, parallel grooves are formed in the storage table surface [1.4], and the sample bin conveying module [6.6] and the recovery bin storage and conveying module [6.4] are installed through fixing pieces. The sample lifting module [6.1] and the recovery lifting module [6.2] are a pair of grooved guide rails, are arranged between the working table surface [1.3] and the storage table surface [1.4], and are vertical to the table surfaces. The sample lifting platform [6.5] and the recovery lifting platform [6.3] are respectively used for fixing a sample vessel and a recovery vessel, and move on the sample bin conveying module [6.6], the recovery bin storage conveying module [6.4], the sample lifting module [6.1] and the recovery lifting module [6.2] through the sliding blocks, wherein the sample vessel passes through the sample vessel ascending channel [11] to reach the working table surface [1.3], and the recovery vessel is recovered from the working table surface [1.3] to the storage table surface [1.4] through the recovery vessel descending channel [13 ]. The conveyer belt of the sample bin conveying module [6.6] is slightly higher than the upper table surface of the storage table surface [1.4] so that the dish can be pressed on the conveyer belt. And when the sample lifting platform [6.5] is arranged on a slide block of the sample lifting module [6.1] and descends to the lowest point of the stroke, the platform surface is lower than the storage platform surface [1.4] by a certain distance. When the lifting platform rises to the highest point of the stroke, the table top is higher than the working table top by a certain distance. The middle of the workbench is designed to be hollow, and the lifting operation can be carried out on the dish by passing through the rear end of the sample bin conveying module [6.6 ]. Guide plates are distributed on two sides of the sample bin conveying module [6.6] in parallel along the grooves to prevent the dish from toppling left and right, and the guide plates are perpendicular to the storage table surface [1.4 ].

The sample vessels are stored on the sample storehouse storage and conveying module [6.6] and can store a plurality of stacks of sample vessels. The sample vessel is moved to the rear end of the sample storage and conveying module [6.6] through the matching of the sample storage and conveying module [6.6] and the sensor, the sample lifting platform [6.5] of the sample lifting module [6.1] lifts the bottommost part of the sample vessel from the bottom and moves to the cover taking height along the sample lifting channel [11], the sample cover moving module [3.4] moves to the cover taking position (namely above the sample lifting channel [11 ]), the vessel cover is clamped through the sample cover clamping jaw [3.3], the sample lifting module [6.1] descends to complete the cover opening action, and the sample cover moving module [3.4] moves the vessel cover to the recovery descending channel [13 ]. And the sample moving bottom die set [3.1] moves to the sample taking position (namely above the sample lifting channel [11 ]), and the sample clamp claw [3.2] clamps the opened sample vessel. The sample moving bottom die set [3.1] moves to a sample photographing and seed taking position [12], a sample dish clamping jaw [3.2] is loosened, and a sample dish is placed on the seed taking identification rotary die set [9 ].

The main body of the seed taking and identifying rotary module [9] is a [9.4] rotary table fixing seat, and the rotary table fixing seat is arranged on the lower table top of the working table top [1.3] through the [9.4] rotary table fixing seat. The structure of the seed-taking identification rotating module [9] is shown in fig. 6 and mainly comprises a driving part and a wheel set, wherein the driving device comprises a [9.1] rotary table motor, a [9.2] motor fixing seat and a [9.3] driving wheel, the [9.1] rotary table motor is fixedly connected with the [9.3] driving wheel and is installed on a [9.4] rotary table fixing seat through the [9.2] motor fixing seat. The wheel set comprises a [9.5] rotary table and a [9.6] driven wheel, the [9.5] rotary table and the [9.6] driven wheel are fixedly connected, the [9.5] rotary table is arranged on a working table surface [1.3] and is higher than the working table surface [1.3] by a certain height and used for rotatably holding and rotating the sample vessel, and the [9.3] driving wheel is connected with the [9.6] driven wheel through a belt.

And (3) rotating a rotary table [9.5] in the seed identification rotary module [9], enabling the side-surface code-sprayed image of the sample vessel to enter a culture vessel periphery code-sprayed identification camera [4.3] through a light path 2, and analyzing in image information to obtain the code of the sample vessel. The dish bottom mark enters the culture dish bottom mark recognition camera [4.5] through the optical path 3. And obtaining the information of the bottom of the sample dish. The sample information on the front of the sample vessel enters a microorganism sample recognition camera [4.1] through an optical path 1. The system automatically selects the microbial colonies with higher purity according to the information, determines the positions of the microbial colonies, and extracts the microbial samples through the seed taking device of the scribing module [2 ].

After the seeds are taken, the sample vessel becomes a recovery vessel for recovery. The sample moving bottom die set [3.1] carries the dish to the recovery dish descending channel [13], the recovery dish lifting module [6.2] ascends to receive the bottom of the dish, and the sample clamping dish clamping claw [3.2] loosens to place the dish. And the sample cover moving module [3.4] moves to the recovery dish descending channel [13], and the sample cover clamping jaw [3.3] loosens and releases the cover to complete the cover closing action. The recycling lifting module [6.2] moves the recycling dish to the recycling conveying module [6.4 ].

The culture dish lifting storage module [10] is positioned on the right side of the storage table top [1.4], and comprises a plate lifting module [10.1], a plate storage device [10.2], a culture dish lifting module [10.3] and a culture dish storage device [10.4], wherein the plate lifting module [10.1], the plate storage device [10.2] is positioned below a plate lifting channel [14], the culture dish lifting module [10.3] and the culture dish storage device [10.4] are positioned below a culture dish descending channel [17 ]. The plate lifting module [10.1] comprises a lifting platform for clamping a plate and a guide rail for operating the lifting platform, is arranged between the storage table surface [1.4] and the working table surface [1.3] and is vertical to the working table surface [1.3], and can move between the storage table surface [1.4] and the working table surface [1.3] through the guide rail. The plate storage device [10.2] is arranged on a storage plane [1.4], and a plurality of storage channels are distributed on the plate storage device and are uniformly distributed in a circumferential array. The culture dish lifting module [10.3] is arranged between the storage table surface [1.4] and the working table surface [1.3] and is vertical to the working table surface [1.3] and comprises a lifting platform for clamping a culture dish and a guide rail for operating the lifting platform. The culture dish storage devices [10.4] are arranged on the storage plane [1.4], storage channels are uniformly distributed in a circumferential array on the culture dish storage devices, and the lifting platform of the lifting module [10.3] can move between the storage platform surface [1.4] and the working platform surface [1.3 ].

The culture dish in the dish storage device [10.2] is moved to a dish cover taking position by a dish lifting module [10.1], a dish cover moving module [8.2] is moved above a dish lifting channel [14], a dish cover is clamped by a dish clamping cover clamping jaw [8.1], the dish lifting module [10.1] descends to realize the cover opening action, and the dish cover moving module [8.2] moves above a culture dish recovery channel [17 ]. The plate moving bottom die set [8.3] moves to the position above the plate lifting channel [14] to take a plate, and a plate clamping bottom clamping jaw [8.4] clamps the plate to finish the plate taking action; the plate bottom moving die set [8.3] moves to the position above the scribing rotary table [15], the plate bottom clamping jaw [8.4] is loosened, and the scribing action is finished by the scribing die set [2 ].

At the moment, the plate becomes an inoculated culture dish, the culture dish is shifted to an ink jet coding table [16] by a scribing dish shifting module [8.5], a corresponding code is generated by the system, the ink jet printer [7] carries out real-time rotary ink jet, and the culture dish is moved to a culture dish descending channel [17] by the ink jet dish shifting module [8.6] after the coding is finished. And (3) loosening a cover clamping jaw (3.3) of the plate cover moving module (3.4) to buckle the dish cover on the dish to finish the closing action of the dish cover. The culture dish is moved by a culture dish lifting module [10.3] to a culture dish storage device [10.4 ].

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A microbial purification instrument is characterized by comprising a rack, a scribing module, a sample carrying switch cover module, a visual camera system, a sample storage lifting module, an ink-jet printer, a culture dish carrying module, a seed taking identification rotating module and a culture dish storage lifting module;

the scribing module is located above the working table.

2. The microorganism purifying apparatus as claimed in claim 1, wherein the top of the microorganism purifying apparatus is provided with a control system, the control system is used for driving, and the control and data display of the microorganism purifying apparatus can be performed through a liquid crystal display screen arranged on the top of the microorganism purifying apparatus;

preferably, the top of the microbial purification instrument is also provided with an ultraviolet disinfection lamp, the ultraviolet disinfection lamp is positioned above the working table, and the working table is illuminated to cover after being started;

or the working table surface and the frame are movably connected, and four corners of the working table surface are movably connected with the frame of the frame, so that the leveling of the working table surface or the height adjustment of the working table surface is convenient to realize.

3. The microbial purification apparatus of claim 1, wherein the left side of the work table has three holes, the centers of the three holes are collinear, and the sample dish ascending channel, the sample photographing and seed taking table and the recovery dish descending channel are arranged from left to right, wherein the sample dish ascending channel and the recovery dish descending channel are sized to allow the culture dish to pass through; the sample carrying, cover opening and cover closing module is fixed on the working table surface, comprises two groups of slide rails and clamps fixed on the slide rails, and respectively comprises a sample bottom moving module, a sample clamp dish clamping jaw, a sample clamp cover clamping jaw and a sample cover moving module, wherein the sample bottom moving module and the sample cover moving module are a pair of slide rails arranged in parallel and are distributed on two sides of a sample dish lifting channel, a sample photographing and seed taking table and a recovery dish descending channel, and the sample clamp dish clamping jaw is fixed on the sample bottom moving module and can move along the sample bottom moving module; the sample cover clamping jaw is fixed on the sample cover moving module and can move along the sample cover moving module; there is the difference in height between sample clamp closure clamping jaw and the sample presss from both sides the ware jack catch.

4. The microbe purifying instrument as claimed in claim 1, wherein the right side of the working table is opened with two holes for accommodating the culture dish, a dish ascending channel and a dish descending channel, a scribing workbench and an ink-jet coding table at one side of the central line of the two holes, the right side of the working table is further provided with a culture dish carrying module comprising four slide rails and two grippers, respectively a dish cover clamping jaw, a dish cover moving module, a dish bottom clamping jaw, a scribing dish shifting module and an ink-jet dish shifting module, the dish cover clamping jaw is mounted on the slide block of the dish cover moving module, and the dish bottom clamping jaw is mounted on the slide block of the dish bottom moving module;

furthermore, the centers of the scribing worktable and the ink-jet coding table are positioned on the extension lines of the centers of three holes of the sample vessel ascending channel, the sample photographing and seed taking table and the recovery vessel descending channel; the center connecting lines of the scribing workbench, the ink-jet coding table, the plate ascending channel and the culture dish descending channel form a rectangle, and the plate cover moving module, the plate bottom clamping jaw, the scribing dish shifting module and the ink-jet dish shifting module are parallel to the rectangle and positioned on the outer side of the rectangle; the plate dish moving bottom die set is positioned on the outer sides of the plate dish lifting channel and the scribing inoculation table, the plate dish moving cover module is positioned on the outer sides of the plate dish lifting channel and the culture dish descending channel, the scribing dish-poking module is positioned on the outer sides of the scribing rotary table and the ink-jet rotary table, and the ink-jet dish-poking module is positioned on the outer sides of the ink-jet rotary table and the culture dish descending channel;

further, an ink-jet printer is arranged beside the ink-jet printing table and used for carrying out ink-jet marking on the culture dish after lineation, and a nozzle of the ink-jet printer is tangent to the edge of the ink-jet rotating table;

furthermore, the main body of the seed taking identification rotating module is a rotary table fixing seat and is arranged at the lower table surface of the working table; the seed taking identification rotating module comprises a driving part and a wheel set, the driving device comprises a rotary table motor, a motor fixing seat and a driving wheel, the rotary table motor is fixedly connected with the driving wheel and is arranged on the rotary table fixing seat through the motor fixing seat; the wheel set comprises a rotary table and a driven wheel, the rotary table is fixedly connected with the driven wheel, the rotary table is arranged on the working table and is higher than the working table by a certain height, the rotary table is used for rotatably containing and rotating the sample vessel, and the driving wheel is connected with the driven wheel through a belt.

5. The microbial purification apparatus of claim 1, wherein the scribing module is fixed on the frame above the worktable and comprises X, Y, R axes of three mechanical arms, wherein the X axis is fixedly connected with the frame, the Y axis is connected with a slide block arranged on the X axis and can slide along the X axis, the R axis is arranged on the slide block of the Y axis and can slide along the Y axis, and the R axis is loaded with the seed taking device; the Y-axis sliding block is connected with the motor reducer of the R axis by a connecting piece and is used for controlling the seed taking device to be close to or far away from the working table surface.

6. The microbe purifier of claim 1, wherein the vision camera system comprises a microbe sample recognition camera, a prism, a culture dish periphery code-spraying recognition camera, a plane mirror, a culture dish bottom mark recognition camera, and a camera fixing frame; the microorganism sample identification camera is fixed right above the sample photographing seed taking table, and light sources are uniformly distributed from the periphery of the camera to irradiate the sample vessel; the plane mirror is arranged right below the sample photographing and seed taking table, and the prism penetrates through the working table surface and is positioned between the sample photographing and seed taking table and the sample cover moving module; the culture dish periphery code-spraying recognition camera is fixed below the working table board, is at the same horizontal position with the prism, and is used for reading the culture dish periphery code-spraying; the culture dish bottom mark identification camera is also arranged below the working table surface and is at the same horizontal position with the plane mirror for reading the culture dish bottom mark.

7. The microbial purification apparatus of claim 1, wherein the sample storage, transportation, elevation and recovery module comprises a sample elevation module, a recovery elevation platform, a recovery bin storage and transportation module, a sample elevation platform, a sample bin storage and transportation module; the sample bin conveying module and the recycling bin storage conveying module are a pair of conveying belts, parallel grooves are formed in the storage table surface, and the sample bin conveying module and the recycling bin storage conveying module are installed through fixing pieces; the sample lifting module and the recovery lifting module are a pair of grooved guide rails, are arranged between the working table top and the storage table top and are vertically arranged; the sample lifting table and the recovery lifting table are respectively used for fixing a sample vessel and a recovery vessel, and move on the sample bin conveying module, the recovery bin storage and conveying module, the sample lifting module and the recovery lifting module through the sliding blocks so as to realize the transfer and recovery of the sample vessel;

8. The microbial purification instrument of claim 1, wherein the dish lifting storage module comprises a dish lifting module, a dish storage device, a dish lifting module, and a dish storage device; the plate lifting module comprises a lifting platform for clamping a plate and a guide rail for operating the lifting platform, is arranged between the storage table surface and the working table surface, is vertical to the working table surface, and can move between the storage table surface and the working table surface through the guide rail; the plate storage device is arranged on the storage plane, and a plurality of storage channels are distributed on the plate storage device and are uniformly distributed in a circumferential array; the culture dish lifting module is arranged between the storage table surface and the working table surface, is vertical to the working table surface, and comprises a lifting table for clamping a culture dish and a guide rail for the operation of the lifting table; the culture dish storage device is arranged on the storage plane, the storage channels are uniformly distributed in a circumferential array manner, and the lifting platform of the lifting module can move between the storage platform surface and the working platform surface.

9. The microbial purification instrument of claim 1, further comprising a seed dispenser recovery bin, wherein the seed dispenser recovery bin is fixed on the working table and is a tank body for recovering the used seed dispenser.

10. The microbial purification apparatus of any one of claims 1-9, wherein the apparatus operates as follows: the sample vessel is stored on the sample bin storage and conveying module, the sample vessel is moved to the rear end of the sample bin storage and conveying module through the cooperation of the sample bin storage and conveying module and the sensor, the sample lifting table of the sample lifting module lifts the bottommost part of the sample vessel from the bottom and moves to the working table surface along the sample lifting channel, the sample vessel is opened through the sample carrying, cover opening and closing module, the sample vessel is moved to the sample photographing and seed taking position, the sample vessel clamping claw is loosened, and the sample vessel is placed on the seed taking identification and rotation module; the seed taking and identifying rotary module rotates to enable the visual camera system to identify codes on the side surface and the bottom of the sample vessel, sample information on the front surface of the sample vessel enters the microbial sample identifying camera, the system automatically selects a microbial colony with higher purity according to the information, determines the position of the microbial colony, and extracts a microbial sample through the seed taking device of the scribing module; the sample vessel after the seed taking is changed into a recovery vessel, and the recovery vessel is moved to a recovery conveying module through a sample storage, conveying, lifting and recovery module; blank culture dish of plate storage device storage is moved to table surface and is accomplished uncapping by culture dish lift storage module, accomplishes the inoculation at the streak inoculation platform, later removes to the inkjet coding platform and carries out the coding, retrieves to storing the mesa by culture dish decline passageway after the coding is accomplished.