CN117264752A - Automatic operating system for screening bacteria and purifying cultured bacteria - Google Patents
Automatic operating system for screening bacteria and purifying cultured bacteria Download PDFInfo
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- CN117264752A CN117264752A CN202311219952.8A CN202311219952A CN117264752A CN 117264752 A CN117264752 A CN 117264752A CN 202311219952 A CN202311219952 A CN 202311219952A CN 117264752 A CN117264752 A CN 117264752A
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- 238000012216 screening Methods 0.000 title claims abstract description 11
- 230000001954 sterilising effect Effects 0.000 claims abstract description 49
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 39
- 230000001580 bacterial effect Effects 0.000 claims abstract description 10
- 239000001963 growth medium Substances 0.000 claims abstract description 10
- 238000011081 inoculation Methods 0.000 claims abstract description 5
- 238000012546 transfer Methods 0.000 claims description 27
- 241000233866 Fungi Species 0.000 claims description 25
- 239000007921 spray Substances 0.000 claims description 17
- 238000005406 washing Methods 0.000 claims description 15
- 230000005540 biological transmission Effects 0.000 claims description 11
- 239000002775 capsule Substances 0.000 claims description 7
- 238000012544 monitoring process Methods 0.000 claims description 7
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/02—Cleaning by the force of jets or sprays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F26—DRYING
- F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
- F26B21/00—Arrangements or duct systems, e.g. in combination with pallet boxes, for supplying and controlling air or gases for drying solid materials or objects
- F26B21/004—Nozzle assemblies; Air knives; Air distributors; Blow boxes
Abstract
The invention discloses an automatic operation system for screening bacteria and purifying cultured bacteria, wherein when the bacteria receiving device receives a bacteria receiving instruction sent by a controller, bacteria receiving operation is performed, the unsealing dish cover is detached from the bacteria-carrying culture dish, and the bacteria receiving device takes down bacterial colonies at the bottom of the bacteria-carrying dish and receives a culture medium in the bottom of the dish to be received; when the first sterilization device receives a sterilization instruction sent by the controller, the sterilization operation is executed for the bacteria receiving device which has executed the bacteria receiving operation. The invention can realize the technical effects of integrating automatic inoculation and sterilization and screening bacteria, save labor force and select bacteria with different forms.
Description
Technical Field
The invention relates to the technical field of microbial experiment instruments, in particular to an automatic operating system for screening bacteria and purifying cultured bacteria.
Background
The microorganism includes: bacteria, viruses, fungi and some small protozoa, microalgae, etc., and its individual is tiny and closely related to human. A plurality of harmful species are covered, and the food, medicine, industry and agriculture, environmental protection, sports and other fields are widely related. In textbooks in our country, microorganisms are classified into the following 8 major categories: bacteria, viruses, fungi, actinomycetes, rickettsia, mycoplasma, chlamydia, spirochetes. Some microorganisms are visible to the naked eye like mushrooms, ganoderma lucidum, mushrooms, etc. which are fungi. Also microorganisms are a class of "non-cellular organisms" consisting of a few components, such as nucleic acids and proteins.
There are many strains of special function in nature, for example, decomposing bacteria that decompose corn straw fibers are typically screened in straw soil, while starch decomposing bacteria are screened in soil near starch plants. In order to screen specific strains, the strains need to be screened in soil, ocean and other environments, the strains in nature are particularly various, the specific strains are screened from the strains, the purification culture process is very complicated, the culture dish needs to be cleaned and sterilized, and the like, and the culture dish needs to be manually cleaned and sterilized, so that the time and the energy of workers are greatly occupied.
Therefore, it is highly desirable to provide an automated operation system for biological screening, purification culture, and cleaning sterilization.
Disclosure of Invention
In view of this, the present invention provides an automated operating system for screening and purifying cultured bacteria. The purpose is to solve the above-mentioned shortcomings.
In order to solve the technical problems, the invention adopts the following technical scheme:
an automated operating system for screening and purifying culture bacteria, which conveys culture dishes to be inoculated and bacteria-carrying culture dishes, wherein the culture dishes to be inoculated comprise: the packaging device comprises a to-be-packaged dish cover and a to-be-connected dish bottom, wherein the to-be-connected dish bottom is sterile; the bacteria-carrying culture dish comprises: unsealing a dish cover and a bacteria-carrying dish bottom, wherein bacteria are carried in the bacteria-carrying dish bottom;
characterized in that the system comprises: the device comprises a controller, a to-be-packaged dish cover conveying assembly, a to-be-connected dish bottom conveying assembly, a bacteria receiving device and a first sterilization device, wherein the to-be-packaged dish cover conveying assembly, the to-be-connected dish bottom conveying assembly, the bacteria receiving device and the first sterilization device are respectively and electrically connected with the controller;
the to-be-packaged dish cover conveying assembly and the to-be-received dish bottom conveying assembly are arranged in the same direction;
the bacteria receiving device is adjacent to the transmission inlet end of the dish cover transporting assembly to be packaged and the transmission inlet end of the dish bottom transporting assembly to be received;
the first sterilization device is arranged adjacent to the bacteria receiving device;
when the bacteria receiving device receives a bacteria receiving instruction sent by the controller, bacteria receiving operation is performed, the unsealing dish cover is detached from the bacteria-carrying culture dish, and the bacteria receiving device takes down a bacterial colony at the bottom of the bacteria-carrying dish and receives the bacterial colony on a culture medium in the bottom of the dish to be received; when the first sterilization device receives a sterilization instruction sent by the controller, the sterilization operation is executed for the bacteria receiving device which has executed the bacteria receiving operation.
Preferably, the apparatus further comprises a first transfer device adjacent to the transport entry end of the capsule transport assembly to be packaged and the transport entry end of the capsule bottom transport assembly to be received;
the first transfer device receives a first transfer instruction sent by the controller, and transfers the dish cover to be packaged to the bottom of the dish to be connected to be combined into the culture dish to be inoculated.
Preferably, the device further comprises a culture dish collecting device, the culture dish collecting device is correspondingly arranged with the conveying output end of the dish bottom conveying assembly to be connected, and the culture dish collecting device is used for collecting the combined culture dishes to be inoculated.
Preferably, the first sterilizing device is an electric flame gun or an ultraviolet emitter.
Preferably, the transport assembly for the dish cover to be packaged is connected with a first position sensor, and the first position sensor is used for sending the monitored position information of the dish cover to be packaged to the controller, so that the controller can control the transport assembly for the dish cover to be packaged to start or pause according to the position information of the dish cover to be packaged.
Preferably, the end part of the bacteria receiving device is provided with a bacteria receiving head, and the bacteria receiving head is a bacteria receiving needle or ring.
Preferably, the device further comprises a bacteria-carrying culture dish conveying assembly, a bacteria-carrying dish bottom conveying assembly, a unsealing dish cover conveying assembly, a second transferring device and an automatic spray washing cabinet;
the tail output end of the bacteria-carrying culture dish conveying assembly is correspondingly arranged with the input end of the unsealing dish cover conveying assembly;
the transport assembly with the bacteria dish bottom is parallel to the transport assembly with the unsealed dish cover;
the second transfer device is adjacent to the tail output end of the culture dish carrying assembly and the input end of the unsealing dish cover carrying assembly, and is used for transferring the unsealing dish cover on the culture dish carrying assembly to the bottom carrying assembly and transferring the bottom of the culture dish on the culture dish carrying assembly to the bottom carrying assembly;
the automatic spray washing cabinet is arranged above the carrier assembly of the bottom of the bacteria-carrying dish and the carrier assembly of the cover of the unsealed dish, and is used for cleaning and sterilizing the cover of the unsealed dish on the carrier assembly of the bottom of the bacteria-carrying dish and the carrier assembly of the cover of the unsealed dish.
Preferably, a hot air device is arranged in the automatic spray washing cabinet, and an air outlet of the hot air device faces to the unsealed dish cover on the transportation assembly with the bottom of the bacteria-carrying dish on the transportation assembly with the unsealed dish cover.
Preferably, the device further comprises a dish cover collecting device and a dish bottom collecting device,
the dish cover collecting device is arranged at the outgoing end of the bacteria-carrying dish bottom conveying assembly and is used for collecting the unsealed dish cover on the bacteria-carrying dish bottom conveying assembly;
the collection device at the bottom of the dish set up in the outgoing end of unsealing dish lid transportation subassembly, collection device at the bottom of the dish is used for collecting unsealing dish lid transportation subassembly is last take the fungus dish to end.
Preferably, the bacteria-carrying culture dish transporting assembly is connected with a second position sensor, the second position sensor is used for monitoring the bottom position of the bacteria-carrying dish transported by the bacteria-carrying culture dish transporting assembly, and the second position sensor sends the bottom position information of the bacteria-carrying dish transported by the bacteria-carrying culture dish transporting assembly to the controller to control the starting or stopping of the bacteria-carrying culture dish transporting assembly;
the transportation assembly at the bottom of the bacteria-carrying dish is connected with a third position sensor, and the third position sensor is used for monitoring the position of the unsealed dish cover transported by the transportation assembly at the bottom of the bacteria-carrying dish and controlling the transportation assembly at the bottom of the bacteria-carrying dish to start or pause.
Compared with the prior art, the invention has the following technical effects:
1. receiving a bacteria receiving instruction sent by the controller through the bacteria receiving device, executing bacteria receiving operation, detaching the unsealing dish cover from the bacteria-carrying culture dish, and taking down the bacterial colony at the bottom of the bacteria-carrying dish by the bacteria receiving device and receiving the bacterial colony on the culture medium in the bottom of the dish to be received; when the first sterilization device receives a sterilization instruction sent by the controller, the sterilization operation is performed for the bacteria receiving device which performs the bacteria receiving operation, so that the technical effects of automatically receiving and sterilizing into a whole and screening bacteria are achieved, labor is saved, and bacteria with different forms are selected;
2. the first position sensor sends position information to the controller, and the sensor automatically controls starting or stopping, so that the personnel can be reduced to watch the whole device;
3. the second position sensor sends position information to the controller, and the controller automatically controls starting or stopping, so that the attention of personnel to the operation of the whole device is further reduced;
4. the automatic spray washing cabinet is used for cleaning and sterilizing, and the hot air device in the automatic spray washing cabinet is matched for blowing hot air, so that the technical effects of integrating automatic cleaning and sterilizing with hot air drying are realized.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a schematic view of the cooperation of the support frame and the frame of the present invention;
FIG. 3 is another schematic view of the present invention from the perspective of FIG. 2;
fig. 4 is a schematic view of a first transfer device according to embodiment 2 of the present invention.
FIG. 5 is a schematic structural diagram of embodiment 3 of the present invention;
FIG. 6 is a schematic diagram of a constant volume pipetting assembly of the invention;
in the figure:
100-a culture dish to be inoculated; 101, a dish cover to be packaged; 102-receiving the bottom of the dish;
700-bacteria-carrying culture dish; 701-unsealing the dish cover; 702-a bottom of the bacteria-carrying dish;
1-a dish cover transportation assembly to be packaged; 111-a first position sensor;
2-a transport assembly at the bottom of the dish to be connected;
3-a bacteria receiving device;
4-a first sterilization device;
5-a first transfer device; 51-connecting seats; 52-rotating the ear;
6-collecting a culture dish device;
7-a bacteria-carrying culture dish transport assembly; 71-a second position sensor;
81-a transport assembly with a bacteria dish bottom; 82-a tamper evident capsule transport assembly; 811-a third position sensor; 9-a second transfer device;
10-an automatic spray washing cabinet;
11-dish cover collection device;
12-a dish bottom collecting device;
13-a bacteria counting device;
15-replacing the fungus heads for later use;
16-a support frame;
17-visual component;
18-ultraviolet germicidal lamp;
191-a first gun; 192-second gun; 193-third gun; 194-gun head base; 195-gun box;
20-marking mechanism.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
Referring to FIGS. 1-3, an automated operating system for screening and purifying culture media is shown, in which a culture dish 100 to be inoculated, a culture dish 700 with bacteria is transported, the culture dish 100 to be inoculated comprising: the container cover 101 to be packaged and the container bottom 102 to be connected are sterile in the container bottom 102 to be connected; the bacteria-carrying culture dish 700 includes: the dish cover 701 is unsealed, the bacteria carrying dish bottom 702 is provided, and bacteria are carried in the bacteria carrying dish bottom 702.
The system comprises: the device comprises a controller, a to-be-packaged dish cover conveying assembly 1, a to-be-connected dish bottom conveying assembly 2, a bacteria receiving device 3 and a first sterilization device 4, wherein the to-be-packaged dish cover conveying assembly, the to-be-connected dish bottom conveying assembly, the bacteria receiving device 3 and the first sterilization device 4 are respectively and electrically connected with the controller;
the to-be-packaged dish cover conveying assembly 1 and the to-be-received dish bottom conveying assembly 2 are arranged in the same direction;
the bacteria receiving device 3 is adjacent to the transmission inlet end of the dish cover conveying assembly 1 to be packaged and the transmission inlet end of the dish bottom conveying assembly 2 to be received;
the first sterilization device 4 is arranged adjacent to the inoculation device 3; when the bacteria receiving device 3 receives a bacteria receiving instruction sent by the controller, a bacteria receiving operation is performed, the unsealing dish cover 701 is detached from the bacteria-carrying culture dish 700, and the bacteria receiving device 3 removes a bacterial colony of the bacteria-carrying dish bottom 702 and receives the bacterial colony on the culture medium in the dish bottom 102 to be received; when the first sterilization device 4 receives a sterilization instruction sent by the controller, the sterilization operation is performed for the fungus receiving device 3 which has performed the fungus receiving operation.
In the embodiment of the invention, when the fungus receiving operation is needed, the fungus receiving instruction can be sent through the controller, so that the fungus receiving device executes the fungus receiving operation. The bacteria receiving operation can enable the carried bacterial colony to complete automatic bacteria receiving operation.
After the fungus picking operation is completed, the controller can send a sterilization instruction to the first sterilization device, so that the first sterilization device sterilizes relevant parts for fungus picking in the fungus picking device.
In the embodiment of the invention, the tray bottom conveying assembly 2 to be connected is controlled by the controller to automatically convey the tray bottom, the tray cover conveying assembly 1 to be packaged is controlled to automatically convey the tray cover, the bacteria receiving device 3 is controlled to automatically receive bacteria for the tray bottom conveyed by the tray cover conveying assembly 2 to be packaged, and the first sterilization device 4 is controlled to sterilize the bacteria receiving device after bacteria receiving, so that the technical effect of integrating automatic bacteria receiving and sterilization is achieved.
In the embodiment of the invention, the bacteria receiving device 3 is provided with a sensor for detecting the position of the bottom of the bacteria receiving dish, when the sensor for detecting the bottom of the bacteria receiving dish detects the position of the bottom of the bacteria receiving dish, whether the position meets the first control condition is determined, if the position meets the first control condition, the transport assembly 1 of the dish cover to be packaged is controlled to stop the transmission operation, and the bacteria receiving device 3 is used for detecting the bacteria receiving at the bottom of the bacteria receiving dish, so that the accuracy of bacteria receiving is improved. The first control condition may be that when the position of the dish bottom is at a specific position, the specific position may be set according to actual needs, so long as the bacteria receiving operation can be accurately performed.
In order to achieve the technical effects that the dish cover transport assembly 1 to be packaged automatically runs and does not need to take care of operation manually, the dish cover transport assembly 1 to be packaged is connected with the first position sensor 111, the first position sensor 111 is arranged at the top end of the frame and is close to the position of the dish cover transport assembly 1 to be packaged, the first position sensor 111 is used for monitoring the position of a dish cover, the first position sensor 111 is electrically connected with the controller and sends dish cover position information to the controller, and the controller controls the dish cover transport assembly 1 to be packaged to start or stop when receiving the dish cover position information, so that the technical effects that automatic operation is achieved and the operation care of personnel to the whole device is reduced are achieved.
In the embodiment of the invention, the bacteria-receiving device 3 may be a telescopic rod or a mechanical arm, and the end of the telescopic rod or the mechanical arm is provided with a bacteria-receiving head, which may be a bacteria-receiving needle.
In the embodiment of the present invention, the first sterilization device 4 is an electric flame gun or an ultraviolet emitter.
When sterilization device 4 is electronic flaming gun, wait to encapsulate dish lid transportation subassembly 1 and wait to connect dish lid electric roller group, wait to connect dish lid electric roller group high temperature resistant to avoided electronic flaming gun to driven influence, and wait to connect dish lid electric roller group operation steady, the defect that does not skid. The electric flame gun receives a sterilization instruction of the controller, and sprays flame to the bacteria receiving device 3, so that the mixed bacteria on the metal material bacteria receiving needle or the coating rod can be eliminated;
when the sterilization device 4 is an ultraviolet emitter, the to-be-packaged dish cover conveying assembly 1 is a to-be-connected dish cover conveying belt. The ultraviolet transmitter receives the sterilization instruction of the controller, and irradiates ultraviolet rays to the bacteria receiving device 3 to eliminate the miscellaneous bacteria on the metal material needle or the coating rod.
In the embodiment of the invention, the bacteria counting device 13 is arranged between the bacteria receiving device 3 and the first transferring device 5, and the bacteria counting device 13 is used for counting the number of microorganisms on the culture medium after bacteria are received and before packaging.
The fungus device 13 is an XK97-A semiautomatic bacteria inspection instrument quantity tester, the fungus of the fungus receiving device 3 is a liquid diluted microorganism, and the statistic quantity is favorable for subsequent microorganism culture and scientific experiments.
The device also comprises a first transfer device 5, wherein the first transfer device 5 is adjacent to the transmission inlet end of the dish cover conveying assembly 1 to be packaged and the transmission inlet end of the dish bottom conveying assembly 2 to be connected;
the first transferring device 5 receives a first transferring instruction sent by the controller, and the first transferring device 5 transfers the dish cover 101 to be packaged to the dish bottom 102 to be connected to be combined into the dish 100 to be inoculated.
The device also comprises a collection culture dish device 6, wherein the collection culture dish device 6 is correspondingly arranged with the transmission output end of the to-be-inoculated culture dish bottom conveying assembly 2, and the collection culture dish device 6 is used for collecting the combined to-be-inoculated culture dishes 100.
The first sterilizing device 4 is an electric flame gun or an ultraviolet emitter.
The to-be-packaged dish cover transporting assembly 1 is connected with a first position sensor 111, and the first position sensor 111 is used for sending the monitored position information of the to-be-packaged dish cover 101 to the controller, so that the controller can control the to-be-packaged dish cover transporting assembly 1 to start or stop according to the position information of the to-be-packaged dish cover 101.
The end part of the bacteria receiving device 3 is provided with a bacteria receiving head which is a bacteria receiving needle or ring.
In the embodiment of the invention, the dish cover transporting component 1 to be packaged can be a dish cover driving belt to be connected or an electric roller set of the dish cover to be connected.
When the first sterilization device 4 is an electric flame gun, the to-be-packaged dish cover transportation assembly 1 is an electric roller set of the to-be-connected dish cover, and the electric roller set at the bottom of the dish is high-temperature resistant, so that the influence of the electric flame gun on transmission is avoided;
when the first sterilization device 4 is an ultraviolet emitter, the to-be-packaged dish cover conveying assembly 1 is a to-be-connected dish cover conveying belt, and the to-be-connected dish cover conveying belt has the advantages of being simple in structure and convenient to adjust.
The first transferring device 5 may be a first pneumatic clamping and grabbing device, where a clamping end of the first pneumatic clamping and grabbing device is provided with a plurality of air discs, when the first pneumatic clamping and grabbing device receives an instruction for transferring the dish cover of the dish cover transporting assembly 1 to be packaged, the plurality of air discs of the first pneumatic clamping and grabbing device absorb the dish cover of the dish cover transporting assembly 1 to be packaged and transfer the dish cover to the dish bottom after the inoculation of the dish bottom transporting assembly 2 to be connected, and the plurality of air discs of the clamping end of the second pneumatic clamping and grabbing device are combined into a culture dish, so that the technical effect of avoiding damage to the dish cover of the dish cover transporting assembly 1 to be packaged is achieved.
The collecting culture dish device 6 is arranged corresponding to the outgoing end of the to-be-received dish bottom conveying assembly 2, and the collecting culture dish device 6 is used for collecting the combined culture dishes. The culture dish collecting device 6 is used for collecting the culture dishes, and the combined culture dishes are piled together, so that the technical effect of avoiding manual operation is achieved.
The device also comprises a bacteria-carrying culture dish conveying assembly 7, a bacteria-carrying dish bottom conveying assembly 81, a unsealing dish cover conveying assembly 82, a second transferring device 9 and an automatic spray washing cabinet 10;
the tail output end of the bacteria-carrying culture dish conveying assembly 7 is correspondingly arranged with the input end of the unsealing dish cover conveying assembly 82;
the carrier assembly 81 with the bacteria bottom is parallel to the carrier assembly 82 with the unsealed vessel cover;
the second transfer device 9 is adjacent to the end output end of the petri dish transporting assembly 7 and the input end of the unsealed dish cover transporting assembly 82, and the second transfer device 9 is used for transferring the unsealed dish cover 701 on the petri dish transporting assembly 7 to the petri dish bottom transporting assembly 81 and transferring the petri dish bottom 702 on the petri dish transporting assembly 7 to the unsealed dish cover transporting assembly 82;
the automatic spray washing cabinet 10 is arranged above the bacteria-carrying dish bottom conveying assembly 81 and the unsealing dish cover conveying assembly 82, and the automatic spray washing cabinet 10 is used for cleaning and sterilizing the unsealing dish cover 701 on the bacteria-carrying dish bottom conveying assembly 81 and the bacteria-carrying dish bottom 702 on the unsealing dish cover conveying assembly 82.
In the embodiment of the invention, the second transferring device 9 may be a second pneumatic clamping and grabbing device, and the clamping end of the second pneumatic clamping and grabbing device is provided with a plurality of air trays, when the second pneumatic clamping and grabbing device receives the instruction of transferring the dish cover of the dish transporting assembly 7, the plurality of air trays of the second pneumatic clamping and grabbing device absorb the dish cover of the dish transporting assembly 7 and transfer the dish cover to the transporting assembly 81 with the bacteria dish bottom, and the technical effect of avoiding damage to the dish cover of the dish transporting assembly 7 is achieved through the plurality of air trays of the clamping end of the second pneumatic clamping and grabbing device.
The automatic spray washing cabinet 10 is internally provided with a hot air device, and the air outlet of the hot air device faces to the unsealed dish cover 701 on the transportation assembly 81 of the bottom of the bacteria-carrying dish and the bottom 702 of the bacteria-carrying dish on the transportation assembly 82 of the unsealed dish cover.
The device also comprises a dish cover collecting device 11 and a dish bottom collecting device 12, wherein the dish cover collecting device 11 is arranged at the outgoing end of the conveying assembly 81 with the fungus dish bottom, and the dish cover collecting device 11 is used for collecting the unsealed dish cover 701 on the conveying assembly 81 with the fungus dish bottom; the bottom collection device 12 is disposed at the outgoing end of the unsealed vessel lid transport assembly 82, and the bottom collection device 12 is configured to collect the bacteria-carrying bottom 702 on the unsealed vessel lid transport assembly 82.
The dish lid collection device 11 sets up in the outgoing end of taking the transportation subassembly of fungus dish end 81, and dish lid collection device 11 is used for collecting the dish lid of taking transportation subassembly of fungus dish end 81. The dish cover collecting device 11 is a dish cover stacker, and the dish covers with the bacteria dish bottom conveying components 81 are stacked together, so that the technical effect of avoiding manual operation is achieved.
The dish bottom collecting device 12 is arranged at the outgoing end of the bacteria-carrying culture dish conveying assembly 7, and the dish bottom collecting device 12 is used for collecting the split dish bottom of the bacteria-carrying culture dish conveying assembly 7. The dish bottom collecting device 12 is a dish bottom stacker, and the split dish bottoms of the bacteria-carrying culture dish conveying assembly 7 are stacked together, so that the technical effect of avoiding manual operation is achieved.
The bacteria culture dish conveying assembly 7 is connected with a second position sensor 71, the second position sensor 71 is used for monitoring the position of the bacteria culture dish bottom 702 conveyed by the bacteria culture dish conveying assembly 7, and the second position sensor 71 sends the position information of the bacteria culture dish bottom 702 conveyed by the bacteria culture dish conveying assembly 7 to the controller to control the bacteria culture dish conveying assembly 7 to start or stop;
the bottom carrier assembly 81 is connected with a third position sensor 811, and the third position sensor 811 is used for monitoring the position of the unsealed dish cover 701 transported by the bottom carrier assembly 81 and controlling the bottom carrier assembly 81 to start or stop.
In the embodiment of the invention, the filter screen is arranged below the automatic spray washing cabinet 10 and is used for filtering and discharging the sterilizing liquid downwards, so that the influence of accumulated water on the top end of the rack is avoided.
The automatic spray washing cabinet is internally provided with a hot air device, and the air outlet of the hot air device faces to the split dish bottom and the split dish cover, so that the technical effect of keeping dry is achieved.
In this embodiment, a supporting frame 16 is connected to the rack, a top frame of the supporting frame 16 is connected to a vision component 17, the vision component 17 faces the rack, the vision component 17 performs image data acquisition, and colonies of the culture dishes to be picked up are identified and screened through correlation among fungus image acquisition, image position identification and fungus colony size identification.
Five rows of parallel ultraviolet germicidal lamps 18 are arranged on the inner side of the top frame of the supporting frame 16, and the radiation ends of the five rows of ultraviolet germicidal lamps 18 face the frame for eliminating bacteria.
Example 2
Referring to fig. 4, on the basis of embodiment 1, the first transfer device 5 includes: the rotary ear 52 is driven to rotate by the output end of the rotary motor extending to the outer side of the connecting seat 51 and connected with the rotary ear 52. The bottom end of the rotary ear 52 is connected with a suction cup. After grabbing the dish cover, the dish cover can be inclined at an angle, so that a large amount of air is prevented from entering the culture dish, and after line drawing is completed, the dish cover can be aligned and covered on the culture dish.
Example 3
Referring to fig. 5 and 6, on the basis of embodiment 2, the present invention further includes: a constant volume pipetting assembly and marking mechanism 20, the constant volume pipetting assembly comprising: first gun 191, second gun 192 and third gun 193, wherein first gun 191 is preferred to transfer 1ml of bacteria to a constant volume, second gun 192 is preferred to transfer 9ml of sterile water to a constant volume, and third gun 193 is preferred for dilution coating. When a diluting and coating instruction is received, the operation is performed on the empty dish bottom, 1ml of bacterial suspension is removed by the first gun 191, 9ml of sterile water is removed by the second gun 192, the two are mixed, the concentration is reduced to 1/10, 1ml of target culture medium placed on the target dish bottom on the conveying component 2 of the dish bottom to be received is sucked from the container, and the container is covered and then is sent to collection culture. The dilution process is repeated again to reduce the concentration, 1ml of the diluted solution is absorbed and then coated into the target culture medium at the bottom of the target dish on the conveying component 2 at the bottom of the dish to be received, and the dilution coating process is sequentially realized.
In this embodiment, the first gun 191, the second gun 192 and the third gun 193 are preferably inserted in the gun mount 194, and the gun mount 194 is adjacent to the bacteria receiving device 3. A gun box 195 is arranged beside the gun head seat 194, and the gun box 195 is used for recycling and placing the disposable gun head.
The marking mechanism 20 is used for diluting the coated culture dish and carrying out coding differentiation on colonies needing to be diluted and coated.
The foregoing description is only of the preferred embodiments of the present invention and is not intended to limit the technical scope of the present invention, so that any minor modifications, equivalent changes and modifications made to the above embodiments according to the technical principles of the present invention still fall within the scope of the technical solutions of the present invention.
Claims (10)
1. An automated handling system for screening and purifying culture media, in which a culture dish (100) to be inoculated, a culture dish with bacteria (700) are transported, said culture dish (100) to be inoculated comprising: the packaging device comprises a dish cover (101) to be packaged and a dish bottom (102) to be connected, wherein the dish bottom (102) to be connected is sterile; the bacteria-carrying culture dish (700) comprises: the device comprises a unsealing vessel cover (701) and a bacteria carrying vessel bottom (702), wherein bacteria are carried in the bacteria carrying vessel bottom (702);
characterized in that the system comprises: the device comprises a controller, a to-be-packaged dish cover conveying assembly (1), a to-be-connected dish bottom conveying assembly (2), a bacteria inoculation device (3) and a first sterilization device (4), wherein the to-be-packaged dish cover conveying assembly, the to-be-connected dish bottom conveying assembly, the bacteria inoculation device (3) and the first sterilization device (4) are respectively and electrically connected with the controller;
the to-be-packaged dish cover conveying assembly (1) and the to-be-received dish bottom conveying assembly (2) are arranged in the same direction;
the bacteria receiving device (3) is adjacent to the transmission inlet end of the dish cover transport assembly (1) to be packaged and the transmission inlet end of the dish bottom transport assembly (2) to be received;
the first sterilization device (4) is arranged adjacent to the bacteria receiving device (3);
when the bacteria receiving device (3) receives a bacteria receiving instruction sent by the controller, bacteria receiving operation is executed, the unsealing dish cover (701) is detached from the bacteria carrying culture dish (700), and the bacteria receiving device (3) removes a bacterial colony of the bacteria carrying dish bottom (702) and receives a culture medium in the dish bottom (102) to be received; when the first sterilization device (4) receives a sterilization instruction sent by the controller, the sterilization operation is performed for the bacteria receiving device (3) which performs the bacteria receiving operation.
2. The device according to claim 1, characterized in that it further comprises first transfer means (5), said first transfer means (5) being adjacent to the transport entry end of the capsule-to-be-packaged transport assembly (1) and to the transport entry end of the capsule-to-be-received transport assembly (2);
the first transfer device (5) receives a first transfer instruction sent by the controller, and the first transfer device (5) transfers the dish cover (101) to be packaged to the dish bottom (102) to be connected to be combined into the dish (100) to be inoculated.
3. The device according to claim 1, further comprising a collection culture dish device (6), wherein the collection culture dish device (6) is arranged corresponding to the delivery output end of the dish bottom transport assembly (2), and the collection culture dish device (6) is used for collecting the combined culture dishes (100) to be inoculated.
4. The device according to claim 1, characterized in that the first sterilizing device (4) is an electric flame gun or an ultraviolet emitter.
5. The device according to claim 1, wherein the to-be-packaged capsule transport assembly (1) is connected with a first position sensor (111), and the first position sensor (111) is used for sending monitored position information of the to-be-packaged capsule (101) to the controller, so that the controller can control the to-be-packaged capsule transport assembly (1) to start or stop according to the position information of the to-be-packaged capsule (101).
6. The device according to claim 1, characterized in that the end of the bacteria-receiving device (3) is provided with a bacteria-receiving head, which is a bacteria-receiving needle or ring.
7. The device according to claim 1, further comprising a petri dish transport assembly (7), a petri dish bottom transport assembly (81), a unsealed dish cover transport assembly (82), a second transfer device (9) and an automatic spray booth (10);
the tail output end of the bacteria-carrying culture dish conveying assembly (7) is arranged corresponding to the input end of the unsealing dish cover conveying assembly (82);
the carrier assembly (81) with the bacteria dish bottom is parallel to the carrier assembly (82) with the unsealed dish cover;
the second transfer device (9) is adjacent to the end output end of the bacteria culture dish conveying assembly (7) and the input end of the unsealing dish cover conveying assembly (82), and the second transfer device (9) is used for transferring the unsealing dish cover (701) on the bacteria culture dish conveying assembly (7) to the bacteria dish bottom conveying assembly (81) and transferring the bacteria dish bottom (702) on the bacteria culture dish conveying assembly (7) to the unsealing dish cover conveying assembly (82);
the automatic spray washing cabinet (10) is arranged above the bacteria-carrying dish bottom conveying assembly (81) and the unsealing dish cover conveying assembly (82), and the automatic spray washing cabinet (10) is used for cleaning and sterilizing the unsealing dish cover (701) on the bacteria-carrying dish bottom conveying assembly (81) and the bacteria-carrying dish bottom (702) on the unsealing dish cover conveying assembly (82).
8. The device according to claim 7, wherein a hot air device is arranged in the automatic spray washing cabinet (10), and an air outlet of the hot air device faces to the unsealed dish cover (701) on the transportation assembly (81) of the bottom of the dish and the bottom (702) of the dish on the transportation assembly (82) of the unsealed dish cover.
9. The device according to claim 7, further comprising a dish cover collecting device (11) and a dish bottom collecting device (12),
the dish cover collecting device (11) is arranged at the outgoing end of the conveying assembly (81) with the fungus dish bottom, and the dish cover collecting device (11) is used for collecting the unsealed dish cover (701) on the conveying assembly (81) with the fungus dish bottom;
the dish bottom collecting device (12) is arranged at the delivery end of the unsealing dish cover transporting assembly (82), and the dish bottom collecting device (12) is used for collecting the bacteria-carrying dish bottom (702) on the unsealing dish cover transporting assembly (82).
10. The device according to claim 7, wherein a second position sensor (71) is connected to the culture dish carrying assembly (7), the second position sensor (71) is used for monitoring the position of the culture dish bottom (702) carried by the culture dish carrying assembly (7), and the second position sensor (71) sends the position information of the culture dish bottom (702) carried by the culture dish carrying assembly (7) to the controller, so as to control the start or stop of the culture dish carrying assembly (7);
the transporting assembly (81) at the bottom of the bacteria-carrying dish is connected with a third position sensor (811), and the third position sensor (811) is used for monitoring the position of the unsealed dish cover (701) transported by the transporting assembly (81) at the bottom of the bacteria-carrying dish and controlling the transporting assembly (81) at the bottom of the bacteria-carrying dish to start or stop.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311219952.8A CN117264752A (en) | 2023-09-21 | 2023-09-21 | Automatic operating system for screening bacteria and purifying cultured bacteria |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311219952.8A CN117264752A (en) | 2023-09-21 | 2023-09-21 | Automatic operating system for screening bacteria and purifying cultured bacteria |
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| CN202311219952.8A Pending CN117264752A (en) | 2023-09-21 | 2023-09-21 | Automatic operating system for screening bacteria and purifying cultured bacteria |
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| JPH0348498U (en) * | 1989-09-14 | 1991-05-09 | ||
| CN107118950A (en) * | 2017-05-06 | 2017-09-01 | 尹康康 | A kind of bacterium automatic vaccination instrument |
| CN109342140A (en) * | 2018-10-30 | 2019-02-15 | 济南百博生物技术股份有限公司 | A kind of high-effective microorganism system for pretreating sample |
| CN114806849A (en) * | 2022-05-31 | 2022-07-29 | 锐视智能(济南)技术研究中心(有限合伙) | Microbial purification appearance |
| WO2022217950A1 (en) * | 2021-04-14 | 2022-10-20 | 中国科学院深圳先进技术研究院 | Automatic colony picking device |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0348498U (en) * | 1989-09-14 | 1991-05-09 | ||
| CN107118950A (en) * | 2017-05-06 | 2017-09-01 | 尹康康 | A kind of bacterium automatic vaccination instrument |
| CN109342140A (en) * | 2018-10-30 | 2019-02-15 | 济南百博生物技术股份有限公司 | A kind of high-effective microorganism system for pretreating sample |
| WO2022217950A1 (en) * | 2021-04-14 | 2022-10-20 | 中国科学院深圳先进技术研究院 | Automatic colony picking device |
| CN114806849A (en) * | 2022-05-31 | 2022-07-29 | 锐视智能(济南)技术研究中心(有限合伙) | Microbial purification appearance |
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