CN114793813B - Drought resistance evaluation method for sugarcane fine-stem wild germplasm resources - Google Patents
Drought resistance evaluation method for sugarcane fine-stem wild germplasm resources Download PDFInfo
- Publication number
- CN114793813B CN114793813B CN202210561328.5A CN202210561328A CN114793813B CN 114793813 B CN114793813 B CN 114793813B CN 202210561328 A CN202210561328 A CN 202210561328A CN 114793813 B CN114793813 B CN 114793813B
- Authority
- CN
- China
- Prior art keywords
- drought
- tested
- drought resistance
- test material
- test
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000011156 evaluation Methods 0.000 title claims abstract description 52
- 240000000111 Saccharum officinarum Species 0.000 title claims abstract description 41
- 235000007201 Saccharum officinarum Nutrition 0.000 title claims abstract description 41
- 238000012360 testing method Methods 0.000 claims abstract description 133
- 239000000463 material Substances 0.000 claims abstract description 95
- 230000004083 survival effect Effects 0.000 claims abstract description 59
- 230000006378 damage Effects 0.000 claims abstract description 46
- 238000004519 manufacturing process Methods 0.000 claims abstract description 33
- 241000196324 Embryophyta Species 0.000 claims description 44
- 208000005156 Dehydration Diseases 0.000 claims description 23
- 238000000034 method Methods 0.000 claims description 12
- 238000003973 irrigation Methods 0.000 claims description 9
- 230000002262 irrigation Effects 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 238000005520 cutting process Methods 0.000 claims description 6
- 238000012216 screening Methods 0.000 claims description 3
- 241000209134 Arundinaria Species 0.000 description 6
- 230000008641 drought stress Effects 0.000 description 6
- 230000035882 stress Effects 0.000 description 3
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000013095 identification testing Methods 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 240000005382 Saccharum spontaneum Species 0.000 description 1
- 235000014704 Saccharum spontaneum Nutrition 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000012854 evaluation process Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000003976 plant breeding Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/55—Sugar cane
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06F—ELECTRIC DIGITAL DATA PROCESSING
- G06F17/00—Digital computing or data processing equipment or methods, specially adapted for specific functions
- G06F17/10—Complex mathematical operations
- G06F17/18—Complex mathematical operations for evaluating statistical data, e.g. average values, frequency distributions, probability functions, regression analysis
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Data Mining & Analysis (AREA)
- Forests & Forestry (AREA)
- Mathematical Optimization (AREA)
- Theoretical Computer Science (AREA)
- Biodiversity & Conservation Biology (AREA)
- Mathematical Physics (AREA)
- Pure & Applied Mathematics (AREA)
- Computational Mathematics (AREA)
- Environmental Sciences (AREA)
- Ecology (AREA)
- Botany (AREA)
- Mathematical Analysis (AREA)
- Evolutionary Biology (AREA)
- Operations Research (AREA)
- Probability & Statistics with Applications (AREA)
- Bioinformatics & Computational Biology (AREA)
- Algebra (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Databases & Information Systems (AREA)
- Software Systems (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to a drought resistance evaluation method for sugarcane fine-stem wild germplasm resources, and belongs to the technical field of drought resistance evaluation. The drought survival test is carried out on the test material to be tested, the drought survival rate is determined from the drought survival type evaluation angle, the drought production type test is carried out on the test material to be tested, a plurality of yield property indexes are determined from the drought production type evaluation angle, the drought resistance damage coefficient of each yield property index is calculated, the average membership value is calculated according to the drought survival rate and the drought resistance damage coefficient of each yield property index, the drought resistance level of the test material to be tested is determined according to the average membership value, the drought resistance evaluation is carried out on the combination of the drought resistance survival type and the drought resistance production type, and the drought resistance of the test material to be tested can be accurately determined by utilizing the comprehensive evaluation method of the drought resistance damage coefficient and the membership value so as to screen excellent drought resistance germplasm resources.
Description
Technical Field
The invention relates to the technical field of drought resistance evaluation, in particular to a drought resistance evaluation method for sugarcane fine-stem wild germplasm resources.
Background
Along with the rapid promotion of the urban process and the increasing competition of crops in the sugarcane area in China, the conditions in the sugarcane area are gradually transferred from paddy fields to high-altitude, low-rainfall and soil-barren dry hillsides. According to statistics, more than 70% of the area of the national sugarcane area is a dry sloping field, wherein the unit yield of the sugarcane in the dry sloping field is less than 3.0 tons/mu, and the unit yield of the sugarcane in most of the arid serious sugarcane areas is long-term loitering between 1 ton/mu and 2 tons/mu. Therefore, drought has become one of the main reasons for low yield per unit of sugarcane and poor quality in China. How to solve the problem of low yield of sugarcane under drought stress is an important subject faced in the current production, and the best solution at present is to cultivate new varieties with excellent drought resistance. Therefore, development and utilization of germplasm resources with excellent drought resistance of the sugarcane are developed, drought-resistant breeding level of the sugarcane is improved, and particularly single yield level of the sugarcane in arid and semiarid regions is improved, so that the method is extremely important for guaranteeing sugar safety and agricultural sustainable development.
The sugarcane fine-stalk wild species (Saccharum spontaneum L), also called as cut-stalk, is an important wild parent resource of modern sugarcane varieties and is an important donor parent for variety resistance. The modern sugarcane varieties all contain the dense blood margin of the cut hand, are the most widely and successfully utilized germplasm resources in wild germplasm resources of the sugarcane, are the most representative successful application of the dense cut hand of Java, the dense cut hand of India and the dense cut hand of cliff city, not only broaden the blood margin foundation of the modern sugarcane varieties, but also have breakthrough contribution to improvement of variety yield and resistance. The secret of the cutting hand is very easy to be mixed and fleed around due to the developed underground transverse stem, and the cross species and the materials are easy to be stored in disorder. Therefore, the drought resistance evaluation of the close-cut germplasm resources is developed, a field experimental evaluation method cannot be simply adopted, and a barrel planting experimental method is best adopted, and in the drought resistance evaluation process, the drought resistance evaluation result has one-sided performance and inaccuracy due to the difference of drought resistance index selection, the length of stress time, the evaluation method and the difference of evaluation angles, so that the method is not suitable for the accurate drought resistance evaluation of the sugarcane germplasm resources.
The drought resistance evaluation method disclosed by the invention is tightly combined with the drought resistance production requirement of the sugarcane, combines the drought survival type and the production type, develops the water stress of the cut-hand dense germplasm resource in the whole growth period, and is simple, convenient and easy to operate in the investigation process by taking the yield character as a main component in the drought resistance index selection, so that the drought resistance of the cut-hand dense germplasm resource can be accurately obtained.
Disclosure of Invention
The invention aims to provide a drought resistance evaluation method for a sugarcane fine-stem wild germplasm resource, which can accurately obtain the drought resistance of the sugarcane fine-stem wild germplasm resource.
In order to achieve the above object, the present invention provides the following solutions:
a drought resistance evaluation method for sugarcane fine-stem wild germplasm resources comprises the following steps:
carrying out drought survival type test on the test material to be tested, and determining the drought survival rate of the test material to be tested from the drought resistance survival type evaluation angle; the test material to be tested is a cutting density; the drought survival test comprises water stress treatment in a seedling stage;
carrying out drought production type test on the test material to be tested, determining a plurality of yield property indexes of the test material to be tested from the drought production type evaluation angle, and calculating a drought resistance damage coefficient of each yield property index; the drought production test comprises water stress treatment after a tillering stage; the yield trait indexes comprise plant height, stem diameter, effective stem number, single-stem weight and sugarcane stem yield;
calculating the average membership value of the test material to be tested according to the drought survival rate of the test material to be tested and the drought damage coefficient of each yield property index by combining two drought resistance evaluation angles of drought resistance survival type and drought resistance production type;
and evaluating the drought resistance of the test material to be tested according to the average membership value, and determining the drought resistance level of the test material to be tested.
According to the specific embodiment provided by the invention, the invention discloses the following technical effects:
the invention is used for providing a drought resistance evaluation method for a sugarcane fine-stalk wild germplasm resource, carrying out drought survival test on a material to be tested, determining drought survival rate from drought survival type evaluation angle, carrying out drought production type test on the material to be tested, determining a plurality of yield character indexes from drought production type evaluation angle, calculating drought resistance damage coefficient of each yield character index, calculating average membership value according to the drought survival rate and the drought resistance damage coefficient of each yield character index, evaluating drought resistance of the material to be tested according to the average membership value, determining drought resistance level of the material to be tested, thereby combining drought resistance survival type and drought resistance production type to carry out drought resistance evaluation, and accurately determining drought resistance of the material to be tested by utilizing a comprehensive evaluation method of the drought resistance damage coefficient and membership value so as to screen excellent drought resistance germplasm resources.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
Fig. 1 is a flow chart of the drought resistance evaluation method provided in embodiment 1 of the present invention.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The invention aims to provide a drought resistance evaluation method for a sugarcane fine-stem wild germplasm resource, which can accurately obtain the drought resistance of the sugarcane fine-stem wild germplasm resource.
In order that the above-recited objects, features and advantages of the present invention will become more readily apparent, a more particular description of the invention will be rendered by reference to the appended drawings and appended detailed description.
Example 1:
the embodiment is used for providing a drought resistance evaluation method for sugarcane fine-stalk wild germplasm resources, as shown in fig. 1, comprising the following steps:
s1: carrying out drought survival type test on the test material to be tested, and determining the drought survival rate of the test material to be tested from the drought resistance survival type evaluation angle; the test material to be tested is a cutting density; the drought survival test comprises water stress treatment in a seedling stage;
the test material to be tested in this embodiment may be the density of the cutting hands introduced from abroad (such as thailand, india), or may be the density of the cutting hands of Yunnan, fujian, guangxi, hainan, etc. collected from the wild of the country. The drought survival test of the embodiment can also be called drought survival drought resistance identification test, and the test site is selected to be performed in a drought greenhouse. In S1, performing the drought survival test on the test material may include: and planting a plurality of test materials to be tested in the same single bud amount in a drought greenhouse barrel, wherein N single buds are planted in the drought greenhouse barrel. And (3) normally watering the single buds in the drought greenhouse barrel corresponding to each test material to be tested until the single buds emerge and have 5 leaves, performing water stress treatment until the drought mortality of the seedlings in the drought greenhouse barrel reaches a preset value, and performing irrigation and rehydration.
Specifically, the N may be 12, and the predetermined value may be 50%, and then the drought survival test of the test material may include: and planting a plurality of test materials to be tested in a drought greenhouse barrel in a mode of 12 buds/barrel, wherein the specification of the drought greenhouse barrel can be 60cm or 40cm, and normally watering before emergence of seedlings of each drought greenhouse barrel corresponding to each test material to be tested, so that all single buds can normally grow, and fixing seedlings in a 5-leaf period before the tillering period starts. The growing period of the sugarcane is divided into five stages of seedling stage, tillering stage, jointing stage, elongation stage and maturity stage, the 5-leaf stage seedling stage before the tillering stage starts in this embodiment means that single bud seedling emergence and 5 leaves are provided, namely when the seedling has 5-leaf age, water stress treatment is started, watering is not performed all the time, and drought stress is started during the seedling stage. Irrigation and rehydration are carried out when the overall drought mortality reaches about 50%.
In S1, after the above test, determining the drought survival rate of the test material to be tested from the drought-resistant survival type evaluation perspective may include: for each test material to be tested, counting the number of seedlings in the drought greenhouse barrel before water stress treatment and the number of survival seedlings in the drought greenhouse barrel after irrigation and rehydration, and calculating the drought survival rate of the test material to be tested according to the number of seedlings and the number of survival seedlings, wherein the drought survival rate=the number of survival seedlings/the number of seedlings is 100%, so as to determine the drought survival rate of each test material to be tested.
As an alternative implementation manner, the present embodiment may further perform repeated tests, for each test material to be tested, respectively planting the test material to be tested in a plurality of drought greenhouse barrels, performing the same treatment on single buds in the plurality of drought greenhouse barrels, counting the number of seedlings that emerge from each barrel before drought stress (seedling stage) and the number of survival seedlings that survive after irrigation and rehydration (seedling stage), and calculating an average value of the number of emerging seedlings and an average value of the number of survival seedlings, so as to more accurately calculate the drought survival rate of the test material to be tested. The method can be carried out for 3 times, and the workload and the cost can be reduced for 3 times.
S2: carrying out drought production type test on the test material to be tested, determining a plurality of yield property indexes of the test material to be tested from the drought production type evaluation angle, and calculating a drought resistance damage coefficient of each yield property index; the drought production test comprises water stress treatment after a tillering stage; the yield trait indexes comprise plant height, stem diameter, effective stem number, single-stem weight and sugarcane stem yield;
the drought production type test of the embodiment can also be called a sugarcane production type drought resistance identification test, and the test site is selected to be carried out in a drought greenhouse. Then in S2, performing a drought production test on the test material to be tested may include: for each of the multiple test materials to be tested involved in S1, planting the test materials to be tested in two test barrels with the same single bud amount, wherein M single buds are planted in each test barrel; marking two test barrels as a first barrel and a second barrel respectively, and normally watering single buds in the first barrel until the tillering stage is finished, and performing water stress treatment, wherein water is watered every A day until the buds are ripe; and (3) normally watering the single buds in the second barrel until the tillering stage is finished, and watering every B days until the buds are mature, wherein B is smaller than A. Of course, bud picking screening can be performed after the single buds in the first barrel and the single buds in the second barrel are sprouted, so that the quantity of the single buds remained in the first barrel is the same as that of the single buds remained in the second barrel, and the quantity of the single buds corresponding to each part of test material to be tested is also ensured to be the same.
Specifically, if M is preferably 4, A is preferably 4, and B is preferably 2, the drought production test of the test material may include: for each of the multiple test materials to be tested involved in the step S1, the test materials to be tested are planted in two test barrels in the same single bud, the specification of each test barrel is 35cm x 50cm, 4 single buds are planted in each test barrel, the two test barrels are respectively a water stress barrel (namely a first barrel) and a control barrel (namely a second barrel), normal watering is carried out before emergence, all normal growth of sugarcane seedlings is ensured, bud picking screening after emergence is carried out, 2 healthy seedlings are ensured in each barrel, normal watering is carried out in seedling stage and tillering stage, water stress treatment is carried out after the tillering stage is finished, water is carried out in each 4 days of each water stress barrel, and water is carried out in each 2 days of each control barrel. The water stress is started after the tillering is finished, and the characteristic of the single bud in the seedling stage and the tillering stage is ensured to be fully expressed.
According to the above test method, determining a plurality of yield property indexes of the test material to be tested from the drought-resistant production type evaluation angle, and calculating the drought-resistant damage coefficient of each yield property index may include: for each yield property index, counting a first index value in the first barrel and a second index value in the second barrel, and calculating a drought resistance damage coefficient of the yield property index according to the first index value and the second index value, wherein the drought resistance damage coefficient of the yield property index is specifically obtained by taking the ratio of the first index value to the second index value.
Specifically, the method may include:
(1) Counting the first plant height, the first stem diameter, the first effective stem number, the first single-stem weight and the first sugarcane stem yield of the mature cut stems in the first barrel, and counting the second plant height, the second stem diameter, the second effective stem number, the second single-stem weight and the second sugarcane stem yield of the mature cut stems in the second barrel.
And in the mature period of the plant, counting the plant height, stem diameter, effective stem number, single-stem weight and cane stem yield of each barrel of material. For the plant height and the stem diameter, 3 plants which grow normally are randomly selected in a barrel in the mature period, the plant height and the stem diameter of the 3 plants are measured, the average value of the plant height and the stem diameter of the 3 plants is calculated, and the results of the plant height and the stem diameter are expressed as the average value; for effective stem numbers, investigating the number of plants with the plant height exceeding 100cm in the mature period in the barrel; for single-stem weight, randomly selecting 3 plants in a barrel in the plant maturity period, actually weighing the total weight of the plants, and dividing the sum of the weights of the plants by the total number of the plants to calculate the average weight of the single stem; for cane stalk yield, the weight of all cane stalks in the barrels was measured at plant maturity. The plants at this time are close in shape after the mature plants are cut.
(2) Calculating a plant height drought resistance damage coefficient according to the first plant height and the second plant height; calculating a drought resistance damage coefficient of the stem diameter according to the first stem diameter and the second stem diameter; calculating an effective stem drought resistance damage coefficient according to the first effective stem number and the second effective stem number; calculating a single-stalk weight drought resistance damage coefficient according to the first single-stalk weight and the second single-stalk weight; and calculating drought resistance damage coefficients of the sugarcane stem yield according to the first sugarcane stem yield and the second sugarcane stem yield.
The calculation formula of the drought resistance damage coefficient used in this embodiment is as follows:
wherein DC ij Drought resistance damage coefficients of the j-th yield property index of the i-th test material to be tested, wherein the yield property index comprises plant height, stem diameter, effective stem number, single-stem weight and cane stem yield; x is X ijws The value of the j-th yield property index of the i-th test material to be tested under drought stress, namely the value in the first barrel; x is X ijww The j-th yield property index of the i-th test material to be tested is the value of the j-th yield property index under the normal irrigation condition, namely the value in the second barrel.
According to the above formula, calculating the drought damage coefficient of the plant height according to the first plant height and the second plant height may include: calculating the ratio of the first plant height to the second plant height to obtain the drought resistance damage coefficient of the plant height. Such as: the measured plant height (namely the second plant height) obtained under the normal irrigation condition is 200cm, the measured plant height (namely the first plant height) obtained under the water stress condition is 150cm, and the drought resistance damage coefficient of the plant height is: 150/200=0.75.
As an alternative implementation mode, the embodiment can also be repeated for a plurality of times, and for each test material to be tested, the test design is controlled and drought stress is conducted, in the test process, a plurality of times of repetition exists in a control area and a stress area, and average values of plant height drought resistance damage coefficients, stem diameter drought resistance damage coefficients, effective stem number drought resistance damage coefficients, single-stem weight drought resistance damage coefficients and cane yield drought resistance damage coefficients of a plurality of tests are calculated, so that experimental errors of environmental factors and human factors are reduced. The method can be carried out for 3 times, and the workload and the cost can be reduced for 3 times.
S3: calculating the average membership value of the test material to be tested according to the drought survival rate of the test material to be tested and the drought damage coefficient of each yield property index;
specifically, S3 may include:
(1) Calculating the drought survival rate and the membership value of the drought damage coefficient of each yield property index respectively by using a membership value calculation formula;
the membership value calculation formula used in this embodiment is:
wherein U is ij The index is a membership value of the j index of the i-th test material to be tested, and comprises drought survival rate, plant height drought resistance damage coefficient, stem diameter drought resistance damage coefficient, effective stem number drought resistance damage coefficient, single-stem weight drought resistance damage coefficient and cane stem yield drought resistance damage coefficient; DC (direct current) ij The measured value of the j index of the i-th test material to be tested; DC (direct current) jmin The minimum value of the j index of all the materials to be tested; DC (direct current) jmax Is the maximum value of the j index of all the tested materials.
(2) And calculating the average value of the membership value of the drought damage coefficient of the drought survival rate and each yield property index to obtain the average membership value.
The calculation formula of the average membership value is as follows:
wherein U is i For the average membership value of the ith test material, U i The larger the value is, the stronger the drought resistance is; n is the total number of indicators, n=6 in this embodiment.
S4: and evaluating the drought resistance of the test material to be tested according to the average membership value, and determining the drought resistance level of the test material to be tested.
Specifically, S4 may include:
(1) Judging whether the average membership value is larger than or equal to a first preset value or not to obtain a first judgment result;
(2) If the first judgment result is yes, the drought resistance level of the test material to be tested is extremely high;
(3) If the first judgment result is negative, judging whether the average membership value is greater than or equal to a second preset value, and obtaining a second judgment result; the second preset value is smaller than the first preset value;
(4) If the second judgment result is yes, the drought resistance level of the test material to be tested is strong;
(5) If the second judgment result is negative, judging whether the average membership value is larger than or equal to a third preset value, and obtaining a third judgment result; the third preset value is smaller than the second preset value;
(6) If the third judgment result is yes, the drought resistance level of the test material to be tested is medium;
(7) If the third judgment result is negative, judging whether the average membership value is larger than or equal to a fourth preset value, and obtaining a fourth judgment result; the fourth preset value is smaller than the third preset value;
(8) If the fourth judgment result is yes, the drought resistance level of the test material to be tested is sensitive;
(9) If the fourth judgment result is negative, the drought resistance level of the test material to be tested is high.
Specifically, the first preset value may be 0.7, the second preset value may be 0.6, the third preset value may be 0.4, and the fourth preset value may be 0.2, and the drought resistance level may be divided according to the following table 1.
TABLE 1 drought resistance rating criteria for plants
The method of the embodiment tightly combines drought-resistant production requirements of plants, combines drought survival type and drought production type, develops water stress of the close-cut germplasm resources in the whole growth period, avoids the influence of serious water shortage in a single period on yield, reduces the requirement on growth period consistency, and reduces test errors. Meanwhile, the drought resistance of the compact seed resource of the cutterhead can be accurately obtained by adopting a membership function comprehensive evaluation method based on drought resistance injury coefficients.
In the drought survival test, the drought survival rate is selected as an index, no watering is performed at all times when water stress treatment is started, and irrigation and rehydration are performed when the overall drought mortality reaches about 50%. In drought production type test, 4 buds are planted in each barrel, buds are picked and screened after seedling emergence, 2 healthy seedlings in each barrel are guaranteed, and the bud numbers of each test material to be tested are consistent before stress. In the drought resistance index selection, the yield property is mainly selected, the investigation process is simple and convenient, the operation is easy, due to different drought resistance evaluation angles, the number of buds of each barrel is different, 12 buds of each barrel are drought-resistant survivable, 4 buds of each barrel are drought-resistant producing, barrels with different specifications are selected, barrels with 60cm x 40cm drought survivable selection specifications are selected, and barrels with 35cm x 50cm drought production selection specifications are selected, so that the test is performed better.
In the drought production type test, water stress is controlled in the whole growth period till the test is finished after the tillering period is finished, the water stress is equally distributed in different growth periods, the influence of serious water shortage in a single period on yield is avoided, the requirement on growth period consistency is reduced, and test errors are reduced.
The drought resistance evaluation method combines two aspects of drought resistance survival type and drought resistance production type, closely combines plant breeding requirements, and screens excellent drought resistance germplasm resources by utilizing a drought resistance damage coefficient membership function comprehensive evaluation method in two experiments of full growth period drought stress after the end of plant seedling period extreme water control and tillering period. The embodiment combines drought resistance survival type and drought resistance production type to develop drought resistance evaluation, and the drought resistance evaluation is more comprehensive. The embodiment adopts a membership function comprehensive evaluation method based on drought resistance damage coefficients, namely the membership function comprehensive evaluation method based on the drought resistance damage coefficients, so that the starting points of all the materials to be tested are consistent.
The principles and embodiments of the present invention have been described herein with reference to specific examples, the description of which is intended only to assist in understanding the methods of the present invention and the core ideas thereof; also, it is within the scope of the present invention to be modified by those of ordinary skill in the art in light of the present teachings. In view of the foregoing, this description should not be construed as limiting the invention.
Claims (7)
1. The drought resistance evaluation method for the sugarcane fine-stalk wild germplasm resources is characterized by comprising the following steps of:
carrying out drought survival type test on the test material to be tested, and determining the drought survival rate of the test material to be tested from the drought resistance survival type evaluation angle; the test material to be tested is a cutting density; the drought survival test comprises water stress treatment in a seedling stage;
carrying out drought production type test on the test material to be tested, determining a plurality of yield property indexes of the test material to be tested from the drought production type evaluation angle, and calculating a drought resistance damage coefficient of each yield property index; the drought production test comprises water stress treatment after a tillering stage; the yield trait indexes comprise plant height, stem diameter, effective stem number, single-stem weight and sugarcane stem yield;
calculating the average membership value of the test material to be tested according to the drought survival rate of the test material to be tested and the drought damage coefficient of each yield property index;
evaluating the drought resistance of the test material to be tested according to the average membership value, and determining the drought resistance level of the test material to be tested;
the drought survival test for the test material to be tested specifically comprises the following steps:
planting a plurality of test materials to be tested in the same single bud amount in a drought greenhouse barrel, wherein N single buds are planted in the drought greenhouse barrel; normally watering single buds in drought greenhouse barrels corresponding to each part of the test material to be tested until the single buds emerge and have 5 leaves, performing water stress treatment until the drought mortality of seedlings in the drought greenhouse barrels reaches a preset value, and performing irrigation and rehydration;
the drought production type test for the test material to be tested specifically comprises the following steps:
for each of the plurality of test materials to be tested, planting the test materials to be tested in two test barrels with the same single bud amount, wherein M single buds are planted in each test barrel; the two test barrels are respectively marked as a first barrel and a second barrel; normally watering single buds in the first barrel until the tillering stage is finished, and performing water stress treatment, wherein water is watered every A day until the buds are ripe; normally watering the single buds in the second barrel until the tillering stage is finished, and watering once every B days until the single buds are mature; wherein B is less than A.
2. The drought resistance evaluation method according to claim 1, wherein the determining of the drought survival rate of the test material to be tested from the drought resistance survival evaluation perspective specifically comprises:
counting the number of seedlings in the drought greenhouse barrel before water stress treatment and the number of survival seedlings in the drought greenhouse barrel after irrigation and rehydration for each part of the test material to be tested; and calculating the drought survival rate of the test material to be tested according to the emergence number and the survival number.
3. The drought resistance assessment method according to claim 1, wherein M is less than N.
4. The drought resistance evaluation method according to claim 1, wherein bud picking screening is performed after the single buds in the first barrel and the single buds in the second barrel are sprouted, so that the number of the single buds remaining in the first barrel is the same as the number of the single buds remaining in the second barrel.
5. The drought resistance evaluation method according to claim 1, wherein determining a plurality of yield trait indices of the test material to be tested from a drought resistance production type evaluation angle, and calculating a drought resistance damage coefficient of each of the yield trait indices specifically comprises:
counting a first index value in the first barrel and a second index value in the second barrel for each yield trait index; and calculating drought resistance damage coefficients of the yield property indexes according to the first index values and the second index values.
6. The drought resistance evaluation method according to claim 1, wherein calculating the average membership value of the test material to be tested according to the drought survival rate of the test material to be tested and the drought resistance damage coefficient of each yield trait index specifically comprises:
calculating the drought survival rate and the membership value of the drought damage coefficient of each yield property index respectively by using a membership value calculation formula;
and calculating the average value of the drought survival rate and the membership value of the drought resistance damage coefficient of each yield property index to obtain an average membership value.
7. The drought resistance evaluation method according to claim 1, wherein the evaluating the drought resistance of the test material to be tested according to the average membership value, determining the drought resistance level of the test material to be tested specifically comprises:
judging whether the average membership value is larger than or equal to a first preset value or not to obtain a first judgment result;
if the first judgment result is yes, the drought resistance level of the test material to be tested is extremely high;
if the first judgment result is negative, judging whether the average membership value is larger than or equal to a second preset value, and obtaining a second judgment result; the second preset value is smaller than the first preset value;
if the second judgment result is yes, the drought resistance level of the test material to be tested is strong;
if the second judgment result is negative, judging whether the average membership value is larger than or equal to a third preset value, and obtaining a third judgment result; the third preset value is smaller than the second preset value;
if the third judgment result is yes, the drought resistance level of the test material to be tested is medium;
if the third judgment result is negative, judging whether the average membership value is larger than or equal to a fourth preset value, and obtaining a fourth judgment result; the fourth preset value is smaller than the third preset value;
if the fourth judgment result is yes, the drought resistance level of the test material to be tested is sensitive; and if the fourth judgment result is negative, the drought resistance level of the test material to be tested is high.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210561328.5A CN114793813B (en) | 2022-05-23 | 2022-05-23 | Drought resistance evaluation method for sugarcane fine-stem wild germplasm resources |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210561328.5A CN114793813B (en) | 2022-05-23 | 2022-05-23 | Drought resistance evaluation method for sugarcane fine-stem wild germplasm resources |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114793813A CN114793813A (en) | 2022-07-29 |
| CN114793813B true CN114793813B (en) | 2024-03-19 |
Family
ID=82516640
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210561328.5A Active CN114793813B (en) | 2022-05-23 | 2022-05-23 | Drought resistance evaluation method for sugarcane fine-stem wild germplasm resources |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114793813B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102301878A (en) * | 2011-07-09 | 2012-01-04 | 云南省农业科学院甘蔗研究所 | Method for evaluating drought resistance/drought tolerance of sugarcane seedlings at seedling stage |
| CN106105753A (en) * | 2016-08-08 | 2016-11-16 | 云南省农业科学院甘蔗研究所 | A kind of Caulis Sacchari sinensis barrel plant drought stress test method |
| CN106818101A (en) * | 2016-07-29 | 2017-06-13 | 新疆农业科学院粮食作物研究所 | A kind of method for identifying drought resistance of maize |
| CN107047292A (en) * | 2017-06-08 | 2017-08-18 | 河北省农林科学院旱作农业研究所 | A kind of selection of corn variety |
| AU2020102986A4 (en) * | 2020-10-23 | 2020-12-24 | Yunnan Agricultural University | Method for Cultivating New Germplasm of Drought-resistant and Cold-resistant Sugarcane Containing the Blood Relationship of Erianthus Fulvus |
| CN112314379A (en) * | 2020-11-11 | 2021-02-05 | 广西壮族自治区农业科学院 | Sugarcane barrel-cultivation drought stress test method |
-
2022
- 2022-05-23 CN CN202210561328.5A patent/CN114793813B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102301878A (en) * | 2011-07-09 | 2012-01-04 | 云南省农业科学院甘蔗研究所 | Method for evaluating drought resistance/drought tolerance of sugarcane seedlings at seedling stage |
| CN106818101A (en) * | 2016-07-29 | 2017-06-13 | 新疆农业科学院粮食作物研究所 | A kind of method for identifying drought resistance of maize |
| CN106105753A (en) * | 2016-08-08 | 2016-11-16 | 云南省农业科学院甘蔗研究所 | A kind of Caulis Sacchari sinensis barrel plant drought stress test method |
| CN107047292A (en) * | 2017-06-08 | 2017-08-18 | 河北省农林科学院旱作农业研究所 | A kind of selection of corn variety |
| AU2020102986A4 (en) * | 2020-10-23 | 2020-12-24 | Yunnan Agricultural University | Method for Cultivating New Germplasm of Drought-resistant and Cold-resistant Sugarcane Containing the Blood Relationship of Erianthus Fulvus |
| CN112314379A (en) * | 2020-11-11 | 2021-02-05 | 广西壮族自治区农业科学院 | Sugarcane barrel-cultivation drought stress test method |
Non-Patent Citations (3)
| Title |
|---|
| 水稻品种苗期抗旱性鉴定指标筛选及其综合评价;李艳, 马均, 王贺正, 张荣萍, 李旭毅;西南农业学报(第03期);第250-255页 * |
| 灌溉与干旱条件下甘蔗工农艺性状的相关性分析与品种抗旱性评价;赵俊;白亚东;赵兴东;杨丽华;卢顺才;李秀梅;马云飞;李梅会;王少河;范源洪;;湖南农业大学学报(自然科学版)(06);第579-586页 * |
| 赵俊 ; 白亚东 ; 赵兴东 ; 杨丽华 ; 卢顺才 ; 李秀梅 ; 马云飞 ; 李梅会 ; 王少河 ; 范源洪 ; .灌溉与干旱条件下甘蔗工农艺性状的相关性分析与品种抗旱性评价.湖南农业大学学报(自然科学版).2016,(06),第579-586页. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114793813A (en) | 2022-07-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Dingkuhn et al. | Relationships between upland rice canopy characteristics and weed competitiveness | |
| Gerik et al. | Plant nitrogen status and boll load of cotton | |
| Kamau et al. | Farmers’ participatory selection for early bulking cassava genotypes in semi-arid Eastern Kenya | |
| Fougereux et al. | Water stress during reproductive stages affects seed quality and yield of pea (Pisum sativum L.) | |
| CN108575736B (en) | Method for screening density-resistant high-yield corn strain suitable for grain machine harvesting in field | |
| CN110073974A (en) | It is a kind of to screen the method and its application for resisting cold cotton variety | |
| Dalal et al. | Seasonal variation in growth, leaf physiology and fruit development in Kinnow, a mandarin hybrid | |
| AU2020102107A4 (en) | A method of characterizing rice population quality | |
| CN111436368A (en) | Drought-resistant wheat breeding method | |
| CN105248272A (en) | Breeding method of high-photosynthetic-efficiency core collections of rape | |
| CN113229076A (en) | Wheat breeding method for screening excellent salt-tolerant strains by using salt pond | |
| CN106818100A (en) | A kind of method for screening drought-resistant maize germplasm | |
| CN114793813B (en) | Drought resistance evaluation method for sugarcane fine-stem wild germplasm resources | |
| CN109146270A (en) | Establishment method of vegetable seedling strengthening index | |
| CN111480476A (en) | Comprehensive evaluation method for identifying drought tolerance of coix seed germplasm resources | |
| Zhang et al. | Influence of leaf age, irrigation and fertilization on leaf tensile strength of Cynodon dactylon and Zoysia japonica | |
| CN109041875A (en) | The yield of rape based on economical character and the prediction technique of lodging resistance | |
| CN109430047B (en) | Breeding method of drought-enduring maize inbred line | |
| CN103782782A (en) | Method for identifying late rice head sprouting flowering period drought-enduring type varieties in field | |
| CN109511407B (en) | Method for rapidly identifying drought tolerance of radish | |
| CN107451927B (en) | Method for representing quality of rice population | |
| Balan | Methods of Foliar Surface Determination along the ontogenetic cycle on apple trees | |
| Nwankwo et al. | Increasing the multiplication efficiency of breeder seed tuber white yam (Dioscorea rotundata Poir) clones through vine cuttings. | |
| CN116298092A (en) | Method for measuring drought stress capacity of sugarcane and application thereof | |
| Teague et al. | Interactions of cotton seeding rates and insect pest control in Arkansas cotton grown in different cover crop systems. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |