CN114793759A - Oyster mushroom culture medium prepared from traditional Chinese medicine residues and preparation method thereof - Google Patents
Oyster mushroom culture medium prepared from traditional Chinese medicine residues and preparation method thereof Download PDFInfo
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- 235000001603 Pleurotus ostreatus Nutrition 0.000 title claims abstract description 94
- 239000001963 growth medium Substances 0.000 title claims abstract description 64
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- 238000002360 preparation method Methods 0.000 title abstract description 26
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- 235000020971 citrus fruits Nutrition 0.000 claims abstract description 50
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims abstract description 33
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 33
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 33
- 239000002994 raw material Substances 0.000 claims abstract description 29
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims abstract description 14
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims abstract description 13
- 238000004519 manufacturing process Methods 0.000 claims abstract description 4
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- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 20
- 238000000034 method Methods 0.000 claims description 19
- 230000001954 sterilising effect Effects 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 14
- 238000010298 pulverizing process Methods 0.000 claims description 14
- 238000005303 weighing Methods 0.000 claims description 14
- 239000000758 substrate Substances 0.000 claims description 12
- 238000011081 inoculation Methods 0.000 claims description 8
- 238000000855 fermentation Methods 0.000 claims description 6
- 230000004151 fermentation Effects 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
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- 230000000694 effects Effects 0.000 abstract description 5
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- 239000002075 main ingredient Substances 0.000 description 8
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 238000012986 modification Methods 0.000 description 5
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- 230000001737 promoting effect Effects 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 240000004534 Scutellaria baicalensis Species 0.000 description 2
- 235000017089 Scutellaria baicalensis Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000003139 buffering effect Effects 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
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- 238000012360 testing method Methods 0.000 description 2
- 241000045403 Astragalus propinquus Species 0.000 description 1
- 241000132012 Atractylodes Species 0.000 description 1
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- 235000009685 Crataegus X maligna Nutrition 0.000 description 1
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- 235000009682 Crataegus limnophila Nutrition 0.000 description 1
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- 239000009636 Huang Qi Substances 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 235000014676 Phragmites communis Nutrition 0.000 description 1
- 241001492261 Pleurotaceae Species 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 241000612118 Samolus valerandi Species 0.000 description 1
- 240000001949 Taraxacum officinale Species 0.000 description 1
- 235000005187 Taraxacum officinale ssp. officinale Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
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- 239000003963 antioxidant agent Substances 0.000 description 1
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- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
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- AYOOGWWGECJQPI-NSHDSACASA-N n-[(1s)-1-(5-fluoropyrimidin-2-yl)ethyl]-3-(3-propan-2-yloxy-1h-pyrazol-5-yl)imidazo[4,5-b]pyridin-5-amine Chemical group N1C(OC(C)C)=CC(N2C3=NC(N[C@@H](C)C=4N=CC(F)=CN=4)=CC=C3N=C2)=N1 AYOOGWWGECJQPI-NSHDSACASA-N 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention belongs to the technical field of oyster mushroom production, and particularly relates to an oyster mushroom culture medium prepared from traditional Chinese medicine residues and a preparation method thereof. The oyster mushroom culture medium is prepared from the following raw materials in parts by weight: 60-100 parts of traditional Chinese medicine residues, 30-60 parts of corncobs, 10-30 parts of citrus leaves, 10-30 parts of orange peels, 0.3-0.5 part of dipotassium hydrogen phosphate and 0.3-0.5 part of monopotassium phosphate. The oyster mushroom culture medium and the preparation method provided by the invention can improve the yield of oyster mushrooms, have obvious effect and have good application prospect.
Description
Technical Field
The invention belongs to the technical field of oyster mushroom production, and particularly relates to an oyster mushroom culture medium prepared from traditional Chinese medicine residues and a preparation method thereof.
Background
Pleurotus ostreatus, named Pleurotus ostreatus and Pleurotus ostreatus, is a species of Pleurotaceae of Basidiomycotina, and is most commonly edible in Gray. The oyster mushroom contains rich nutrients such as protein, amino acid and the like, is suitable for various temperatures, can be planted all the year round, and is easy to produce, so that the edible value and the nutritional value are high.
The main factors influencing the yield of the oyster mushroom are strains, culture mediums and cultivation methods, so if the yield of the oyster mushroom is to be improved and economic benefits are to be improved, the three aspects are preferably started. The selection of the strains needs operations such as screening, cultivation, performance identification and the like, the time period is long, and the selection of the strains has uncertainty, so that new strains with improved properties are difficult to breed. The cultivation method is characterized in that the best conditions are provided for the growth and fruiting of the oyster mushrooms by controlling the environmental factors such as the temperature and the humidity of the oyster mushroom growth, the advantages of the existing culture medium of the existing strains are brought into play to the maximum extent, and the yield reduction phenomenon is avoided, namely, the improvement of the cultivation method is mainly to avoid the yield reduction (compared with the yield obtained by adopting the best cultivation method when a scientific research worker screens the oyster mushroom strains and configures a culture medium formula), and the yield is hardly broken through. The culture medium substrate is adjusted, the nutrient formula is improved, the growth of the oyster mushroom is stimulated, and the method is a quick and effective method for improving the yield of the oyster mushroom and is a hot point of research.
The oyster mushroom culture medium in the prior art comprises the following main components: the components of corncob, sawdust, rice chaff and sugar are used as main materials with higher content, so that the main materials are cheap and easy to obtain, but because the culture medium formed by the components has insufficient nutrition, the yield of the oyster mushroom is not high, and therefore, the nutrient supplement containing nutrient substances such as nitrogen, phosphorus, potassium, amino acid and the like is added into the medium, but the range of improving the yield by the nutrient supplement is limited. The general appeal in the field of oyster mushroom cultivation is that the yield of oyster mushrooms is improved, and a culture medium with a new formula capable of improving the yield of oyster mushrooms is continuously developed.
The herb residue is a byproduct of traditional Chinese medicine production, and if recycling of the herb residue can be realized, environmental pollution can be reduced, and waste utilization effect can be achieved, so that a culture medium containing the herb residue and capable of improving yield of oyster mushrooms needs to be provided.
Disclosure of Invention
In order to solve the technical problems, the invention provides an oyster mushroom culture medium prepared from traditional Chinese medicine residues.
The invention aims to provide an oyster mushroom culture medium prepared from traditional Chinese medicine residues, which is prepared from the following raw materials in parts by weight: 60-100 parts of traditional Chinese medicine residues (providing trace element nutrition, lignin and the like), 30-60 parts of corncobs (providing elements such as nitrogen, carbon and the like and providing some trace elements), 10-30 parts of citrus leaves (promoting the growth of oyster mushrooms), 10-30 parts of orange peels (promoting the growth of oyster mushrooms), 0.3-0.5 part of dipotassium phosphate (buffering pH and supplementing potassium) and 0.3-0.5 part of monopotassium phosphate (buffering pH and supplementing potassium).
Preferably, the oyster mushroom culture medium prepared by using the traditional Chinese medicine residues is prepared by drying the residual medicine residues in the process of producing Pudilan oral liquid (Jichuan pharmaceutical industry group limited company, Chinese medicine standard Z20030095, main components of dandelion, bunge corydalis herb, radix isatidis and scutellaria baicalensis) and Jianer digestion-promoting oral liquid (Sichuan Taihuatang pharmaceutical limited company, Chinese medicine standard Z1102093, main components of astragalus mongholicus, bighead atractylodes rhizome, radix ophiopogonis, dried orange peel, semen raphani, hawthorn and scutellaria baicalensis) for later use.
Preferably, the oyster mushroom culture medium prepared from the traditional Chinese medicine residues is prepared from the following raw materials in parts by weight: 60-80 parts of traditional Chinese medicine residues, 30-40 parts of corncobs, 10-20 parts of citrus leaves, 20-30 parts of orange peels, 0.3-0.5 part of dipotassium hydrogen phosphate and 0.3-0.5 part of monopotassium phosphate.
Preferably, the oyster mushroom culture medium prepared from the traditional Chinese medicine residues is prepared from the following raw materials in parts by weight: 70 parts of traditional Chinese medicine residues, 35 parts of corncobs, 15 parts of citrus leaves, 25 parts of orange peels, 0.4 part of dipotassium hydrogen phosphate and 0.4 part of monopotassium phosphate.
Preferably, the preparation method of the oyster mushroom culture medium prepared from the traditional Chinese medicine residues comprises the following steps:
weighing the raw material components in proportion;
respectively pulverizing the Chinese medicinal residue, corn cob, citrus leaf and pericarpium Citri Tangerinae into 1-5mm pieces;
mixing the above granules, adding dipotassium hydrogen phosphate and potassium dihydrogen phosphate, adding water until the water content of the materials is 60-65%, mixing thoroughly to obtain Pleurotus Ostreatus culture medium, and sterilizing.
Preferably, the preparation method of the oyster mushroom culture medium prepared from the traditional Chinese medicine residues comprises the following steps:
weighing the raw material components in proportion;
respectively pulverizing the Chinese medicinal residue, corn cob, citrus leaf and pericarpium Citri Tangerinae into 1-5mm pieces;
mixing the above Chinese medicinal residue and corn cob, fermenting with yeast, mixing the obtained fermented product with mandarin orange leaf, pericarpium Citri Tangerinae, dipotassium hydrogen phosphate and potassium dihydrogen phosphate to obtain Pleurotus Ostreatus culture medium, and sterilizing.
Preferably, in the preparation method of the oyster mushroom culture medium prepared from the Chinese medicine residues, the yeast is saccharomyces cerevisiae CCTCC NO: m2020708.
Preferably, the method for preparing the oyster mushroom culture medium from the herb residue comprises the following steps:
drying the Chinese medicinal residue and corn cob, mixing the dried materials, adding water to the mixture until the water content reaches 40-50%, inoculating yeast, and fermenting at room temperature for 3-5 days to obtain fermented product.
Preferably, in the preparation method of the oyster mushroom culture medium prepared from the traditional Chinese medicine residues, the inoculation amount of the yeast is 3-5%.
Preferably, the preparation method of the oyster mushroom culture medium prepared from the traditional Chinese medicine residues comprises the steps of mixing the fermentation product with citrus leaves and citrus peel, standing for one day, and then fully mixing with dipotassium hydrogen phosphate and potassium dihydrogen phosphate to obtain the oyster mushroom culture medium.
Compared with the prior art, the invention has the following beneficial effects:
1. the oyster mushroom fermentation substrate is prepared from traditional Chinese medicine residues, corncobs, citrus leaves, orange peels, dipotassium hydrogen phosphate and potassium dihydrogen phosphate; the Chinese medicine residue contains abundant trace element nutrition, lignin, etc.; the corncob contains nitrogen, carbon and some trace elements; the citrus leaves and the citrus peel contain antioxidant components and some nutrient elements, and experiments show that the citrus leaves and the citrus peel can accelerate the growth of oyster mushrooms and improve the yield of the oyster mushrooms; dipotassium hydrogen phosphate and potassium dihydrogen phosphate can supplement potassium element and balance the pH value of the culture medium.
2. The invention utilizes the Saccharomyces cerevisiae CCTCC NO: m2020708 ferments traditional Chinese medicine residue and corncob, degrades macromolecular substances therein into micromolecular substances, is convenient for oyster mushroom absorption and utilization, and experimental results show that the growth of oyster mushroom can be accelerated by taking the fermented materials as a part of culture medium, and the yield of oyster mushroom is improved.
3. Compared with the prior art, the oyster mushroom culture medium and the preparation method provided by the invention can improve the yield of oyster mushrooms, have obvious effects and have good application prospects.
Detailed Description
In order that those skilled in the art will better understand the technical solutions of the present invention to practice, the present invention will be further described with reference to the following specific examples.
In the description of the present invention, reagents used are commercially available and methods used are conventional in the art, unless otherwise specified. In the invention, the citrus leaves are leaves remaining after harvesting citrus in the citrus tree cultivation process, healthy leaves without diseases are selected, and the orange peel is fresh orange peel (the peeling time from the orange is not more than 3 days).
Influence of different substrate formulas on growth of oyster mushrooms
Example 1
An oyster mushroom culture medium prepared from traditional Chinese medicine residues is prepared from the following raw materials in parts by weight: 70 parts of traditional Chinese medicine residues, 35 parts of corncobs, 15 parts of citrus leaves, 25 parts of orange peels, 0.4 part of dipotassium hydrogen phosphate and 0.4 part of monopotassium phosphate. The traditional Chinese medicine residue is used for producing Pudilan oral liquid (JICHUAN pharmaceutical industry group, Inc., Chinese medicine standard Z20030095, main ingredients of herba Taraxaci, herba Violae, radix Isatidis and Scutellariae radix), and the residue is air dried for use.
The preparation method comprises the following steps:
weighing the raw material components in proportion;
respectively pulverizing the Chinese medicinal residue, corn cob, citrus leaf and pericarpium Citri Tangerinae into 2-3mm pieces;
mixing the above residues, corncob, citrus leaves and orange peel, adding dipotassium hydrogen phosphate and potassium dihydrogen phosphate, adding water until water content is 60-65%, mixing to obtain Pleurotus Ostreatus culture medium, and steam sterilizing.
Example 2
An oyster mushroom culture medium prepared from traditional Chinese medicine residues is prepared from the following raw materials in parts by weight: 60 parts of traditional Chinese medicine residues, 30 parts of corncobs, 30 parts of citrus leaves, 30 parts of orange peels, 0.5 part of dipotassium hydrogen phosphate and 0.5 part of monopotassium phosphate. The traditional Chinese medicine residue is used for producing Pudilan oral liquid (JICHUAN pharmaceutical industry group, Inc., Chinese medicine standard Z20030095, main ingredients of herba Taraxaci, herba Violae, radix Isatidis and Scutellariae radix), and the residue is air dried for use.
The preparation method comprises the following steps:
weighing the raw material components in proportion;
respectively pulverizing the Chinese medicinal residue, corn cob, citrus leaf and pericarpium Citri Tangerinae into 2-3mm pieces;
mixing the above residues, corncob, citrus leaf and orange peel, adding dipotassium hydrogen phosphate and potassium dihydrogen phosphate, adding water until the water content of the materials is 60-65%, mixing thoroughly to obtain Pleurotus Ostreatus culture medium, and steam sterilizing.
Example 3
An oyster mushroom culture medium prepared from traditional Chinese medicine residues is prepared from the following raw materials in parts by weight: 100 parts of traditional Chinese medicine residues, 60 parts of corncobs, 10 parts of citrus leaves, 10 parts of orange peels, 0.3 part of dipotassium hydrogen phosphate and 0.3 part of monopotassium phosphate. The traditional Chinese medicine residue is used for producing Pudilan oral liquid (JICHUAN pharmaceutical industry group, Inc., Chinese medicine standard Z20030095, main ingredients of herba Taraxaci, herba Violae, radix Isatidis and Scutellariae radix), and the residue is air dried for use.
The preparation method comprises the following steps:
weighing the raw material components in proportion;
respectively pulverizing the Chinese medicinal residue, corn cob, citrus leaf and pericarpium Citri Tangerinae into 2-3mm pieces;
mixing the above residues, corncob, citrus leaf and orange peel, adding dipotassium hydrogen phosphate and potassium dihydrogen phosphate, adding water until the water content of the materials is 60-65%, mixing thoroughly to obtain Pleurotus Ostreatus culture medium, and steam sterilizing.
Example 4
An oyster mushroom culture medium prepared from traditional Chinese medicine residues is prepared from the following raw materials in parts by weight: 80 parts of traditional Chinese medicine residues, 40 parts of corncobs, 20 parts of citrus leaves, 20 parts of orange peels, 0.4 part of dipotassium hydrogen phosphate and 0.4 part of monopotassium phosphate. The traditional Chinese medicine residue is used for producing Pudilan oral liquid (JICHUAN pharmaceutical industry group, Inc., Chinese medicine standard Z20030095, main ingredients of herba Taraxaci, herba Violae, radix Isatidis and Scutellariae radix), and the residue is air dried for use.
The preparation method comprises the following steps:
weighing the raw material components in proportion;
respectively pulverizing the Chinese medicinal residue, corn cob, citrus leaf and pericarpium Citri Tangerinae into 2-3mm pieces;
mixing the above residues, corncob, citrus leaf and orange peel, adding dipotassium hydrogen phosphate and potassium dihydrogen phosphate, adding water until the water content of the materials is 60-65%, mixing thoroughly to obtain Pleurotus Ostreatus culture medium, and steam sterilizing.
Example 5
An oyster mushroom culture medium prepared from traditional Chinese medicine residues is prepared from the following raw materials in parts by weight: 90 parts of traditional Chinese medicine residues, 50 parts of corncobs, 25 parts of citrus leaves, 15 parts of orange peels, 0.4 part of dipotassium hydrogen phosphate and 0.4 part of monopotassium phosphate. The traditional Chinese medicine residue is used for producing Pudilan oral liquid (JICHUAN pharmaceutical industry group, Inc., Chinese medicine standard Z20030095, main ingredients of herba Taraxaci, herba Violae, radix Isatidis and Scutellariae radix), and the residue is air dried for use.
The preparation method comprises the following steps:
weighing the raw material components in proportion;
respectively pulverizing the Chinese medicinal residue, corn cob, citrus leaf and pericarpium Citri Tangerinae into 2-3mm pieces;
mixing the above residues, corncob, citrus leaf and orange peel, adding dipotassium hydrogen phosphate and potassium dihydrogen phosphate, adding water until the water content of the materials is 60-65%, mixing thoroughly to obtain Pleurotus Ostreatus culture medium, and steam sterilizing.
Example 6
An oyster mushroom culture medium prepared from traditional Chinese medicine residues is prepared from the following raw materials in parts by weight: 70 parts of traditional Chinese medicine residues, 35 parts of corncobs, 15 parts of citrus leaves, 25 parts of orange peels, 0.4 part of dipotassium hydrogen phosphate and 0.4 part of monopotassium phosphate. The Chinese medicinal residue is residue of Chinese medicinal decoction for producing children promoting digestion oral liquid (Sichuan Taihuatang pharmaceutical Co., Ltd., Chinese medicine standard Z1102093, main ingredients of radix astragali, Atractylodis rhizoma, radix Ophiopogonis, pericarpium Citri Tangerinae, Raphani semen, fructus crataegi and Scutellariae radix), and air drying the residue for use.
The preparation method comprises the following steps:
weighing the raw material components in proportion;
respectively pulverizing the Chinese medicinal residue, corn cob, citrus leaf and pericarpium Citri Tangerinae into 2-3mm pieces;
mixing the above residues, corncob, citrus leaves and orange peel, adding dipotassium hydrogen phosphate and potassium dihydrogen phosphate, adding water until water content is 60-65%, mixing to obtain Pleurotus Ostreatus culture medium, and steam sterilizing.
Control group 1
An oyster mushroom culture medium prepared from traditional Chinese medicine residues is prepared from the following raw materials in parts by weight: 70 parts of traditional Chinese medicine residues, 35 parts of corncobs, 15 parts of citrus leaves, 0.4 part of dipotassium hydrogen phosphate and 0.4 part of potassium dihydrogen phosphate. The traditional Chinese medicine residue is used for producing Pudilan oral liquid (JICHUAN pharmaceutical industry group, Inc., Chinese medicine standard Z20030095, main ingredients of herba Taraxaci, herba Violae, radix Isatidis and Scutellariae radix), and the residue is air dried for use.
The preparation method comprises the following steps:
weighing the raw material components in proportion;
respectively pulverizing the Chinese medicinal residue, corn cob, citrus leaf and pericarpium Citri Tangerinae into 2-3mm pieces;
mixing the above residues, corncob, citrus leaf and orange peel, adding dipotassium hydrogen phosphate and potassium dihydrogen phosphate, adding water until the water content of the materials is 60-65%, mixing thoroughly to obtain Pleurotus Ostreatus culture medium, and steam sterilizing.
Control group 2
An oyster mushroom culture medium prepared from traditional Chinese medicine residues is prepared from the following raw materials in parts by weight: 70 parts of traditional Chinese medicine residues, 35 parts of corncobs, 15 parts of citrus leaves, 0.4 part of dipotassium hydrogen phosphate and 0.4 part of potassium dihydrogen phosphate. The traditional Chinese medicine residue is used for producing Pudilan oral liquid (JICHUAN pharmaceutical industry group, Inc., Chinese medicine standard Z20030095, main ingredients of herba Taraxaci, herba Violae, radix Isatidis and Scutellariae radix), and the residue is air dried for use.
The preparation method comprises the following steps:
weighing the raw material components in proportion;
respectively pulverizing the Chinese medicinal residue, corn cob, citrus leaf and pericarpium Citri Tangerinae into 2-3mm pieces;
mixing the above residues, corncob, citrus leaf and orange peel, adding dipotassium hydrogen phosphate and potassium dihydrogen phosphate, adding water until the water content of the materials is 60-65%, mixing thoroughly to obtain Pleurotus Ostreatus culture medium, and steam sterilizing.
Control group 3
CN 201410348283.9A Pleurotus ostreatus culture medium containing reed straw and its preparation method, the substrate obtained by the specific embodiment mode of the embodiment.
The substrates of examples 1 to 6 and control groups 1 to 3 were packed in bags, respectively, each containing 2kg of wet weight of the substrate, and 9 parallel tests were prepared for each group. Pleurotus ostreatus was cultivated according to the method of 1.2.3-1.2.4 of Xinlong \31066. See table 1 for results. The results showed that the method of example-6 group had higher average growth rate of the hypha of fresh mushroom.
TABLE 1 Effect of different substrate formulations on the growth of Pleurotus Ostreatus
Secondly, the influence of different preparation methods on the growth of oyster mushrooms
Taking the formulation of example 1 as an example, the influence of different substrate preparation methods on the growth of oyster mushroom was studied.
Control group: preparation of example 1
Experimental group 1: weighing the raw material components according to the proportion of the example 1; respectively pulverizing the Chinese medicinal residue, corn cob, citrus leaf and pericarpium Citri Tangerinae into 2-3mm pieces; drying the Chinese medicinal residue and corn cob in the sun to dry thoroughly (sterilizing with sunlight), mixing the dried materials, adding water to the mixture to make the water content of the materials reach 40-50% (w/w), inoculating yeast, and fermenting at room temperature for 4 days to obtain fermented product. And then, fully mixing the fermentation product with citrus leaves, orange peel, dipotassium hydrogen phosphate and potassium dihydrogen phosphate to obtain the oyster mushroom culture medium, and sterilizing for use.
Wherein, the inoculation amount of the yeast (yeast material) accounts for 3 percent of the mass of the wet material.
The preparation method of the yeast material for inoculation comprises the following steps: the saccharomyces cerevisiae CCTCC NO: m2020708 research on yeast material by using "Xuezhiming. Pleurotus ostreatus liquid strain culture and inoculation automation technology [ D ]. Jiangsu university, 2008" 2.1.
Experimental group 2: weighing the raw material components according to the proportion of the example 1; respectively pulverizing the Chinese medicinal residue, corn cob, citrus leaf and pericarpium Citri Tangerinae into 2-3mm pieces; airing the Chinese medicine residues and the crushed corn cobs until the crushed corn cobs are fully dried (sterilization by sunlight), mixing the dried materials, adding water into the mixture to enable the water content of the materials to reach 40-50% (w/w), inoculating saccharomycetes, fermenting at room temperature for 4 days to obtain a fermented product, mixing the fermented product with citrus leaves and orange peels, standing for one day, fully mixing the fermented product with dipotassium hydrogen phosphate and potassium dihydrogen phosphate to obtain an oyster mushroom culture medium, and sterilizing for use.
Wherein, the inoculation amount of the yeast (yeast material) accounts for 3 percent of the mass of the wet material.
The preparation method of the yeast material for inoculation comprises the following steps: the saccharomyces cerevisiae CCTCC NO: m2020708 research on yeast material by using "Xuezhiming. Pleurotus ostreatus liquid strain culture and inoculation automation technology [ D ]. Jiangsu university, 2008" 2.1.
The substrates of example 1 and experimental groups 1-2 were packed in bags, each containing 2kg of wet weight of the substrate, and 9 parallel tests were prepared for each group. Pleurotus ostreatus was cultivated according to the method of 1.2.3-1.2.4 of Xinlong \31066. See table 2 for results. The result shows that the oyster mushroom culture medium prepared by the fermentation method has higher average growth speed of the fresh mushroom hyphae. Note that table 1 and table 2 are experiments performed in the same batch, and therefore, the weight of the first fresh mushroom, the amount of the second fresh mushroom, the weight of the third fresh mushroom, and the growth rate of the first fresh mushroom mycelia, which were prepared by the method of example 1, were the same.
TABLE 2 Effect of different substrate preparation methods on the growth of Pleurotus Ostreatus
It should be noted that, when the present invention relates to a numerical range, it should be understood that two endpoints of each numerical range and any value between the two endpoints can be selected, and since the steps and methods adopted are the same as those in the embodiment, in order to prevent redundancy, the present invention describes a preferred embodiment. While preferred embodiments of the present invention have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. Therefore, it is intended that the appended claims be interpreted as including preferred embodiments and all such alterations and modifications as fall within the scope of the invention.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (10)
1. The oyster mushroom culture medium prepared from the traditional Chinese medicine residues is characterized by being prepared from the following raw materials in parts by weight: 60-100 parts of traditional Chinese medicine residues, 30-60 parts of corncobs, 10-30 parts of citrus leaves, 10-30 parts of orange peels, 0.3-0.5 part of dipotassium hydrogen phosphate and 0.3-0.5 part of monopotassium phosphate.
2. The oyster mushroom cultivation substrate prepared from the herb residue according to claim 1, wherein the herb residue is residue from the production of Pudilan oral liquid and Jianer Xiaoshi oral liquid, and is dried for later use.
3. The oyster mushroom culture medium prepared from the herb medicine residues according to claim 2, wherein the oyster mushroom culture medium is prepared from the following raw materials in parts by weight: 60-80 parts of traditional Chinese medicine residues, 30-40 parts of corncobs, 10-20 parts of citrus leaves, 20-30 parts of orange peels, 0.3-0.5 part of dipotassium hydrogen phosphate and 0.3-0.5 part of monopotassium phosphate.
4. The oyster mushroom culture medium prepared from the herb medicine residues according to claim 3, wherein the oyster mushroom culture medium is prepared from the following raw materials in parts by weight: 70 parts of traditional Chinese medicine residues, 35 parts of corncobs, 15 parts of citrus leaves, 25 parts of orange peels, 0.4 part of dipotassium hydrogen phosphate and 0.4 part of monopotassium phosphate.
5. The method for preparing a culture medium of Pleurotus Ostreatus prepared from Chinese medicinal residue as claimed in claim 1, comprising the steps of:
weighing the raw material components in proportion;
respectively pulverizing the Chinese medicinal residue, corn cob, citrus leaf and pericarpium Citri Tangerinae into 1-5mm pieces;
mixing the above granules, adding dipotassium hydrogen phosphate and potassium dihydrogen phosphate, adding water until the water content of the materials is 60-65%, mixing thoroughly to obtain Pleurotus Ostreatus culture medium, and sterilizing.
6. The method for preparing a culture medium of Pleurotus Ostreatus prepared from Chinese medicinal residue as claimed in claim 1, comprising the steps of:
weighing the raw material components in proportion;
respectively pulverizing the Chinese medicinal residue, corn cob, citrus leaf and pericarpium Citri Tangerinae into 1-5mm pieces;
mixing the above Chinese medicinal residue and corn cob, fermenting with yeast, mixing the obtained fermented product with mandarin orange leaf, pericarpium Citri Tangerinae, dipotassium hydrogen phosphate and potassium dihydrogen phosphate to obtain Pleurotus Ostreatus culture medium, and sterilizing.
7. The method for preparing Pleurotus ostreatus culture medium using herb residue as claimed in claim 6, wherein the yeast is Saccharomyces cerevisiae CCTCC NO: m2020708.
8. The method for preparing Pleurotus ostreatus culture medium by using herb residue as claimed in claim 7, wherein the fermentation method using yeast is as follows:
drying the Chinese medicinal residue and corn cob, mixing the dried materials, adding water to the mixture until the water content reaches 40-50%, inoculating yeast, and fermenting at room temperature for 3-5 days to obtain fermented product.
9. The method for preparing Pleurotus ostreatus culture medium using herb residue as defined in claim 8, wherein the inoculation amount of yeast is 3-5%.
10. The method for preparing Pleurotus Ostreatus culture medium from Chinese medicinal residue according to claim 9, wherein the fermentation product is mixed with citrus leaf and pericarpium Citri Tangerinae, and then left for one day, and then thoroughly mixed with dipotassium hydrogen phosphate and potassium dihydrogen phosphate to obtain Pleurotus Ostreatus culture medium.
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