CN114717298A - 一种多重扩增方法 - Google Patents

一种多重扩增方法 Download PDF

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Publication number
CN114717298A
CN114717298A CN202110011778.2A CN202110011778A CN114717298A CN 114717298 A CN114717298 A CN 114717298A CN 202110011778 A CN202110011778 A CN 202110011778A CN 114717298 A CN114717298 A CN 114717298A
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CN
China
Prior art keywords
sequence
amplification
nucleic acid
capture oligonucleotide
universal
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Pending
Application number
CN202110011778.2A
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English (en)
Chinese (zh)
Inventor
徐高连
徐宏
杨浩
古宏晨
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Shanghai Huizhong Tongkang Biotechnology Co ltd
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Shanghai Huizhong Tongkang Biotechnology Co ltd
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Publication date
Application filed by Shanghai Huizhong Tongkang Biotechnology Co ltd filed Critical Shanghai Huizhong Tongkang Biotechnology Co ltd
Priority to CN202110011778.2A priority Critical patent/CN114717298A/zh
Priority to PCT/CN2022/070427 priority patent/WO2022148388A1/zh
Priority to DE112022000534.3T priority patent/DE112022000534T5/de
Publication of CN114717298A publication Critical patent/CN114717298A/zh
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/16Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202110011778.2A 2021-01-06 2021-01-06 一种多重扩增方法 Pending CN114717298A (zh)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CN202110011778.2A CN114717298A (zh) 2021-01-06 2021-01-06 一种多重扩增方法
PCT/CN2022/070427 WO2022148388A1 (zh) 2021-01-06 2022-01-06 一种多重扩增方法
DE112022000534.3T DE112022000534T5 (de) 2021-01-06 2022-01-06 Verfahren zur Multiplex-Amplifizierung

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202110011778.2A CN114717298A (zh) 2021-01-06 2021-01-06 一种多重扩增方法

Publications (1)

Publication Number Publication Date
CN114717298A true CN114717298A (zh) 2022-07-08

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ID=82234897

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202110011778.2A Pending CN114717298A (zh) 2021-01-06 2021-01-06 一种多重扩增方法

Country Status (3)

Country Link
CN (1) CN114717298A (de)
DE (1) DE112022000534T5 (de)
WO (1) WO2022148388A1 (de)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5882856A (en) 1995-06-07 1999-03-16 Genzyme Corporation Universal primer sequence for multiplex DNA amplification
US20060216737A1 (en) * 2005-03-10 2006-09-28 John Bodeau Methods for multiplex amplification
CN104694630B (zh) * 2015-02-02 2017-05-24 江苏佰龄全基因生物医学技术有限公司 用于多重连接扩增技术的探针制备方法
CN109477105A (zh) * 2016-05-24 2019-03-15 阿提拉生物系统股份有限公司 Ω扩增
CN108300716B (zh) * 2018-01-05 2020-06-30 武汉康测科技有限公司 接头元件、其应用和基于不对称多重pcr进行靶向测序文库构建的方法
CN108753935A (zh) * 2018-06-15 2018-11-06 上海优甲医疗科技有限公司 一种降低多重聚合酶链式反应中引物二聚体扩增的方法

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Publication number Publication date
DE112022000534T5 (de) 2023-10-26
WO2022148388A1 (zh) 2022-07-14

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Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
EE01 Entry into force of recordation of patent licensing contract

Application publication date: 20220708

Assignee: Mingde Henan Biotechnology Co.,Ltd.

Assignor: Shanghai Huizhong Tongkang Biotechnology Co.,Ltd.

Contract record no.: X2023980041926

Denomination of invention: A Multiple Amplification Method

License type: Common License

Record date: 20230918

EE01 Entry into force of recordation of patent licensing contract