CN114700188B - Detector is collected to gene tissue section centrifugation successive layer - Google Patents

Detector is collected to gene tissue section centrifugation successive layer Download PDF

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Publication number
CN114700188B
CN114700188B CN202210619535.1A CN202210619535A CN114700188B CN 114700188 B CN114700188 B CN 114700188B CN 202210619535 A CN202210619535 A CN 202210619535A CN 114700188 B CN114700188 B CN 114700188B
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groove
centrifugal
extraction
locking
wall
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CN114700188A (en
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张金银
巩赞博
巩赞斌
巩赞华
黄咏华
彭劼
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Huachenyang Shenzhen Technology Co ltd
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Huachenyang Shenzhen Technology Co ltd
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B5/00Other centrifuges
    • B04B5/04Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B04B5/0414Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B11/00Feeding, charging, or discharging bowls
    • B04B11/04Periodical feeding or discharging; Control arrangements therefor
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B11/00Feeding, charging, or discharging bowls
    • B04B11/04Periodical feeding or discharging; Control arrangements therefor
    • B04B2011/046Loading, unloading, manipulating sample containers

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  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention relates to a gene tissue slice centrifugal layer-by-layer collecting detector, which relates to the technical field of gene detection and comprises a detection box body, wherein a centrifugal processing mechanism is arranged in the detection box body, a separation and extraction mechanism is arranged on the detection box body, and the separation and extraction mechanism and the centrifugal processing mechanism are matched with each other to collect gene tissue slices subjected to centrifugal layering.

Description

Detector is collected to gene tissue section centrifugation successive layer
Technical Field
The application relates to the technical field of gene detection, in particular to a gene tissue slice centrifugal layer-by-layer collection detector.
Background
The genetic testing is a process of finding abnormality of a specific genetic material by performing testing and analysis using genetic and molecular biological techniques. Gene detection has been widely used in clinical examination, precision medicine, food inspection and quarantine, species identification, and the like. In the gene detection process, the form and structure of biological tissues or cells are researched by using a tissue slice technology, components in the tissue slices are extracted by mixing special medicaments and slices and adopting a centrifugal technology to detect in the process of detecting the tissue slices at present, the components of a solution in the tissue slices form a layered state due to the difference of the component quality and the density after centrifugation, and more accurate detection can be obtained by collecting liquid in different layers for detection.
In the existing gene detecting apparatus, for example, chinese patent No. CN2110699253A, it discloses a gene detecting system and a gene detecting method, specifically, it includes: a control unit, a reagent container unit, provided with a label; a label recognition unit that recognizes a position of each reagent container; a constant temperature storage unit; a centrifuge; a gene detector; a pipetting assembly; and a turnaround unit; the label identification unit identifies the type and the position of each reagent container in the reagent container unit, then transmits the identified information to the control unit, the control unit analyzes and calculates according to the received information, calculates to obtain an operation program of the mechanical arm and sends a control instruction to the mechanical arm, and the mechanical arm acts according to the control instruction. The gene detection system realizes linkage between the label identification unit and the turnover unit through the control unit, reduces manual participation, reduces labor cost, has the advantages of high working efficiency and low error rate, and can improve the detection efficiency and accuracy of the gene detection system;
in the prior art, the label recognition unit and the mechanical arm are controlled by the control unit to act, the test tube discrimination in the detection operation is controlled by the automatic mechanical action, the steps after the gene detection is finished are further accurately controlled, the situation that the detection result does not correspond to the sample is placed, the preparation operation before the gene detection is not involved, the gene tissue section solution after the layering is extracted in a layering way in the problems to ensure the accuracy of the detection result, at present, when the gene solution for the tissue section is extracted in a layering way, the solutions in different layers are collected and extracted by a needle tube or other tools, when the tools are inserted into the solution, the solution in the previous layer is inevitably brought into the next layer, and the solution in different layers needs to stand for a period of time to wait for the extraction after the separation, such an operation takes a long time and affects the efficiency of gene detection.
Disclosure of Invention
In order to enable the gene tissue slices to accurately and quickly collect the genes layered after centrifugation in the detection process, the application provides a gene tissue slice centrifugation layer-by-layer collection detector.
The application provides a detector is collected to gene tissue section centrifugation successive layer adopts following technical scheme:
including detecting the box, detect the inside centrifugal treatment mechanism that is provided with of box, be provided with separation extraction mechanism on the detection box, separation extraction mechanism and centrifugal treatment mechanism mutually support and gather the gene tissue section after the centrifugation layering:
the centrifugal processing mechanism comprises a drawing plate, a centrifugal motor, a centrifugal rotating plate and a placing sleeve, wherein a limiting chute is formed in the bottom of the detection box body, the drawing plate is arranged in the limiting chute in a sliding mode, the drawing plate is provided with the centrifugal motor through a motor base, the centrifugal rotating plate is arranged on an output shaft of the centrifugal motor, and the placing sleeve for placing a test tube is arranged on the centrifugal rotating plate;
the separation extraction mechanism includes lift cylinder, the crane, the extraction pipe, the butt joint ring, the rubber buffer and extraction branch chain, and lift cylinder installs on the inner wall that detects the box, installs the crane on the lift cylinder, and the extraction pipe is installed to the lower extreme of crane, installs the butt joint ring through the bearing on the outer wall of extraction pipe, installs on the inner wall of butt joint ring to be used for blockking up test tube open-ended rubber buffer, is provided with the extraction branch chain in the extraction pipe, evenly sets up on the crane and extracts branch chain complex lift groove.
By adopting the technical scheme, in the process of actually carrying out centrifugal treatment on the gene tissue slices, the pumping plate is controlled to carry out displacement, the test tube with the gene tissue section can be conveniently placed in the placing sleeve, the test tube is limited by the placing sleeve, the lifting cylinder is started to drive the lifting frame to move downwards so that the extraction tube is inserted into the test tube, and the rubber plug is inserted into the opening position of the test tube, the test tube can be limited, the test tube can be synchronously driven to rotate when the centrifugal motor is started to control the centrifugal rotating plate to rotate, the placing sleeve and the rubber plug are matched to play a role in protecting the test tube in the process, after the centrifugal operation is finished, the layering condition of the gene tissue slice in the test tube is observed, and (4) carrying out positioning collection on the gene tissue slices after layering in the test tube by extracting the branched chains.
Preferably, the upper end face for placing the sleeve is provided with an annular groove, the inner wall of the annular groove is uniformly provided with supporting springs, and the supporting springs are provided with annular plates.
Preferably, place and be provided with the locking branch chain that is used for advancing line location to the test tube on the sleeve pipe, the locking branch chain includes locking installation piece, locking spring, block rubber, connecting rod and the lantern ring, places and evenly begins to have a locking groove along its circumference on the sleeve pipe, and locking installation piece slides and sets up in the locking groove, locking installation piece and place and be provided with locking spring between the sheathed tube outer wall, be provided with the block rubber on the locking installation piece.
Preferably, place and evenly set up the linkage groove with the ring channel intercommunication on the sleeve pipe, the connecting rod slides and sets up in the linkage groove, and the one end of connecting rod is connected on the annular slab, and the other end of connecting rod is installed on the inner wall of the lantern ring.
Preferably, the diameter of the lantern ring increases progressively from top to bottom, and the locking mounting block abuts against the inner wall of the lantern ring.
Through adopting above-mentioned technical scheme, in order to realize the test tube at the stability of rotation process, the accessible docking ring drives connecting rod and lantern ring from last down motion to the extrusion of annular plate in step, and the lantern ring drives the rubber block extrusion on the locking installation piece when the downstream on the outer wall of test tube, can realize the stable locking to the test tube through the rubber block to the multiple spot extrusion of test tube, guarantees the test tube and follows the stability of centrifugal rotor plate at the rotation in-process.
Preferably, place the sleeve pipe and from last down seted up the extraction hole, the extraction hole is the spiral orbit and arranges placing the sleeve pipe, and place set up on the sleeve pipe with extraction hole complex sliding tray.
Preferably, the extraction branch chain comprises a hose, a connecting ring, a piston tube, a closing plate, a connecting frame, a closing spring and a control assembly, the connecting ring is uniformly arranged on the lifting frame, one end of the hose is connected with the extraction hole, the other end of the hose is connected with the connecting ring, the control assembly is arranged on the outer wall of the lifting frame, the piston tube is placed on the control assembly, the pipe orifice of the piston tube is inserted into the connecting ring, the closing plate is arranged in the sliding groove in a sliding mode, the closing spring is arranged between the closing plate and the inner wall of the sliding groove, the connecting frame is arranged on the closing plate, and the connecting frame is connected with the lifting groove in a sliding mode.
Preferably, the control assembly comprises a support bracket, a moving block and a control support, the support bracket is arranged on the lifting frame at equal intervals, the support bracket is used for placing the piston tube, a moving groove is formed in the support bracket, the moving block is arranged in the moving groove in a sliding mode, an arc groove is formed in the moving block, a piston rod on the piston tube is placed in the arc groove, the lower end of the moving block is provided with the control support, and the control support extends into the lifting groove and abuts against the upper end face of the connecting frame.
Through adopting above-mentioned technical scheme, when extracting the genetic tissue section solution after centrifugal treatment in vitro, through observing the layering condition of genetic tissue section solution after centrifugal treatment in vitro, select the piston pipe that corresponds with it, make the closure plate bulk extraction hole of upward movement through the removal of control movable block in the shifting chute, synchronous piston rod extracts the collection through the hose to the solution after the layering when following the movable block displacement, accomplishes the purpose that genetic tissue section layering was gathered.
Preferably, place the sheathed tube right-hand member and offer the rectangular channel that is used for observing the test tube, detect the right-hand member face of box and offer and be used for the viewing aperture, viewing aperture department is provided with glass panels, and glass panels selects convex surface glass, and the front side that detects the box articulates there is the door plant, is provided with the metal strip on the door plant, be provided with on the detection box with metal strip complex magnet strip.
Through the technical scheme, after the gene tissue slices in the test tube are centrifugally operated, the layering condition of the gene tissue slices in the test tube is observed through the glass panel of the convex glass.
To sum up, this application beneficial technology effect does:
carry out centrifugation and layering collection in-process to the gene tissue section in reality, insert test tube open position through the rubber piece, and place the sleeve pipe spacing to the test tube, can improve the stability of test tube in rotating the centrifugation process, gene tissue section in the test tube is through the centrifugation back, accessible glass panels observes the in vitro divided into condition of gene tissue section, the solution of layering is gathered to the sliced concrete position control extraction branch chain of gene tissue according to different levels, through this technical scheme, can guarantee the purity of the gene tissue section solution of gathering, ensure that the gene solution between the different levels can not mix, guarantee the accurate nature of testing result, improve the efficiency that detects.
Drawings
Fig. 1 is a schematic view of the overall structure of the present application.
FIG. 2 is a schematic view showing the structure of the quantitative determination casing, the centrifugal processing mechanism and the separation and extraction mechanism.
Fig. 3 is a schematic structural view of the separating and extracting mechanism.
FIG. 4 is a sectional view of the crane and the extraction branch.
Fig. 5 is a schematic view of the structure between the extraction tube, the docking ring, the rubber stopper, the closing spring and the closing plate.
FIG. 6 is a schematic view of the second embodiment of the present invention showing the structure between the centrifugal rotor plate and the placing sleeve.
Description of the reference numerals: detecting a box body 1; a centrifugal processing mechanism 2; a separating and extracting mechanism 3; a pulling plate 21; a centrifugal motor 22; a centrifugal rotating plate 23; placing a sleeve 24; a lifting cylinder 31; a lifting frame 32; an extraction tube 33; a docking ring, 34; rubber plug, 35; extracting a branched chain 36; a hose, 361; a connection ring, 362; a piston tube, 363; a closure plate, 364; a connecting frame 365; a closing spring, 366; a control assembly, 367; a support bracket 3671; a moving mass 3672; a control bracket, 3673; a locking mounting block, 242; a locking spring, 243; rubber blocks, connecting rods, 244; a collar, 245.
Detailed Description
The present application is described in further detail below with reference to figures 1-6.
The embodiment of the application discloses detector is collected to gene tissue section centrifugation successive layer can carry out accurate collection that draws fast to the gene tissue section of in vitro layering after to gene tissue section centrifugation layering, has improved the accurate nature of gene tissue section in the detection.
The first embodiment is as follows:
referring to fig. 1, for the disclosure of this embodiment, including detecting box 1, detect the inside centrifugal processing mechanism 2 that is provided with of box 1, be provided with separation extraction mechanism 3 on detecting box 1, separation extraction mechanism 3 mutually supports with centrifugal processing mechanism 2 and gathers the gene tissue section after the centrifugation layering:
the front side that detects box 1 articulates there is the door plant, is provided with the metal strip on the door plant, detect box 1 on be provided with metal strip complex magnet strip, place the rectangular channel that the test tube was offered to the right-hand member of sleeve pipe 24, detect box 1's right-hand member face and offer and be used for the viewing aperture, viewing aperture department is provided with glass panels, glass panels selects convex surface glass.
Referring to fig. 2 to 5, in order to better collect gene tissue slices in a test tube after the gene tissue slices are subjected to centrifugal treatment in a layered manner, in the embodiment, a centrifugal treatment mechanism 2 includes a drawing plate 21, a centrifugal motor 22, a centrifugal rotating plate 23 and a placing sleeve 24, a limiting chute is formed at the bottom of a detection box body 1, the drawing plate 21 is slidably arranged in the limiting chute, the drawing plate 21 is provided with the centrifugal motor 22 through a motor base, an output shaft of the centrifugal motor 22 is provided with the centrifugal rotating plate 23, and the placing sleeve 24 for placing the test tube is arranged on the centrifugal rotating plate 23;
separation extraction mechanism 3 includes lift cylinder 31, crane 32, extraction tube 33, butt joint ring 34, rubber buffer 35 and extraction branch chain 36, lift cylinder 31 installs on the inner wall that detects box 1, install crane 32 on the lift cylinder 31, extraction tube 33 is installed to crane 32's lower extreme, install butt joint ring 34 through the bearing on extraction tube 33's the outer wall, install on butt joint ring 34's the inner wall and be used for blockking up test tube open-ended rubber buffer 35, be provided with extraction branch chain 36 in the extraction tube 33, evenly set up on crane 32 with extraction branch chain 36 complex lift groove.
By adopting the technical scheme, in the actual process of carrying out centrifugal treatment on the gene tissue slices, the pulling plate 21 is controlled to displace, a test tube with the gene tissue slices can be conveniently placed in the placing sleeve 24, the test tube is limited by placing the sleeve 24, the pulling plate 21 is pushed to a working position, then the door plate on the detection box body 1 is closed to enable the magnet strip to adsorb the metal strip, the door plate is abutted against the pulling plate 21 to limit the metal strip, the lifting cylinder 31 is started to drive the lifting frame 32 to move downwards to enable the pulling tube 33 to be inserted into the test tube, the rubber plug 35 is inserted into the opening position of the test tube to limit the test tube, the centrifugal motor 22 is started to control the centrifugal rotating plate 23 to rotate, the test tube can be synchronously driven to rotate, and the placing sleeve 24 and the rubber plug 35 are matched to play a protection role in the test tube in the process, after the centrifugal operation is finished, the layering condition of the gene tissue slices in the test tube is observed, and the layered gene tissue slices in the test tube are positioned and collected through the extraction branched chain 36.
Place sleeve pipe 24 and from last down seted up the extraction hole, the extraction hole is the spiral orbit and arranges placing sleeve pipe 24 on, and place sleeve pipe 24 on set up with extraction hole complex sliding tray.
The extraction branched chain 36 comprises a hose 361, a connecting ring 362, a piston tube 363, a closing plate 364, a connecting frame 365, a closing spring 366 and a control assembly 367, wherein the connecting ring 362 is uniformly arranged on the lifting frame 32, one end of the hose 361 is connected with an extraction hole, the other end of the hose 361 is connected with the connecting ring 362, the control assembly 367 is arranged on the outer wall of the lifting frame 32, the piston tube 363 is arranged on the control assembly 367, the nozzle of the piston tube 363 is inserted into the connecting ring 362, the closing plate 364 is arranged in a sliding groove in a sliding manner, the closing spring 366 is arranged between the closing plate 364 and the inner wall of the sliding groove, the connecting frame 365 is arranged on the closing plate 364, and the connecting frame 365 is connected with the lifting groove in a sliding manner.
An annular rubber block is arranged on the inner wall of the connecting ring 362, the cross section of the annular rubber block is of a stepped structure, and the connection position can be sealed by the annular rubber block when the piston tube 363 is in butt joint with the connecting ring 362.
The same mark is used on the corresponding extraction hole and the piston tube 363, for example, a color, number or symbol awakening mark is used, so that the specific situation of the gene tissue slice after centrifugal layering can be accurately judged in the actual operation process, the color difference of the solution at different layers can be generated after the gene tissue slice is centrifugally stood and layered, the corresponding piston tube 363 is selected according to the color difference position corresponding to the extraction hole to extract the solution with different color differences for collection, the accuracy of the collected gene tissue slice solution can be ensured, and the gene tissue slice solution cannot be mixed with components at other layers.
The control component 367 comprises a supporting bracket 3671, a moving block 3672 and a control bracket 3673, the supporting bracket 3671 is arranged on the lifting frame 32 at equal intervals, the supporting bracket 3671 is used for placing a piston tube 363, a moving groove is formed in the supporting bracket 3671, the moving block 3672 is arranged in the moving groove in a sliding mode, an arc groove is formed in the moving block 3672, a piston rod on the piston tube 363 is placed in the arc groove, the control bracket 3673 is arranged at the lower end of the moving block 3672, and the control bracket 3673 extends into the lifting groove and abuts against the upper end face of the connecting frame 365.
Through the technical scheme, after the gene tissue slices in the test tube are centrifugally operated, the layering condition of the gene tissue slices in the test tube is observed through the glass panel of the convex glass.
The control bracket 3673 abuts against the upper end face of the connecting frame 365 in an initial state, at the moment, the connecting frame 365 drives the closing plate 364 to extrude downwards, so that the extraction hole is in a closed state, when the control bracket 3673 moves along with the moving block 3672, the control bracket 3673 contacts and extrudes the connecting frame 365, at the moment, the connecting frame 365 drives the closing plate 364 to move upwards under the action of the closing spring 366, and the piston tube 363 synchronously extracts and collects the gene tissue slices of the designated layers through the extraction tube 33.
By adopting the technical scheme, when the gene tissue slice solution which is subjected to centrifugal treatment in the test tube is extracted, the piston tube 363 corresponding to the centrifugal treatment is selected by observing the layering condition of the gene tissue slice solution which is subjected to centrifugal treatment in the test tube, the closing plate 364 moves upwards through controlling the moving block 3672 to move in the moving groove to form the large extracting hole, the layered solution is extracted into the piston tube 363 through the hose 361 when the synchronous piston rod follows the moving block 3672 to move for collection, and the purpose of layered collection of the gene tissue slice is achieved.
In the second embodiment, referring to fig. 6, in order to improve the stability of the test tubes in the centrifugal operation and enable the test tubes of different specifications to perform stable operation in the present invention on the basis of the first embodiment, in the present embodiment, an annular groove is formed on the upper end surface of the placing sleeve 24, supporting springs are uniformly installed on the inner wall of the annular groove, and an annular plate 241 is installed on the supporting springs;
place and be provided with the locking branch chain that is used for fixing a position the test tube on the sleeve pipe 24, the locking branch chain includes locking installation piece 242, locking spring 243, the rubber piece, connecting rod 244 and lantern ring 245, places and evenly begins to have a locking groove along its circumference on the sleeve pipe 24, and locking installation piece 242 slides and sets up in the locking groove, is provided with locking spring 243 between locking installation piece 242 and the outer wall of placing sleeve pipe 24, is provided with the rubber piece on the locking installation piece 242.
The placing sleeve 24 is uniformly provided with a linkage groove communicated with the annular groove, the connecting rod 244 is arranged in the linkage groove in a sliding manner, one end of the connecting rod 244 is connected to the annular plate 241, and the other end of the connecting rod 244 is arranged on the inner wall of the lantern ring 245.
The collar 245 increases in diameter from top to bottom and the lock mounting block 242 abuts against the inner wall of the collar 245.
When inserting the annular groove in the butt joint ring 34 downstream and extrude annular plate 241 downwards, annular plate 241 drives lantern ring 245 from last down motion in the in-process of downstream in step, lantern ring 245 extrudees locking installation piece 242 when from last down motion, locking installation piece 242 receives to drive the rubber block after the extrusion and extrudees towards the outer wall of test tube, it is spacing to carry out the multiple spot to the test tube through the rubber block, can make the test tube can be stable rotate in the centrifugal processing operation.
Through adopting above-mentioned technical scheme, in order to realize the test tube at the stability of rotation process, accessible butt joint ring 34 drives connecting rod 244 and lantern ring 245 from last down motion to the extrusion of annular plate 241 in step, lantern ring 245 drives the rubber block extrusion on the locking installation piece 242 when the downstream on the outer wall of test tube, can realize the stable locking to the test tube through the rubber block to the multiple spot extrusion of test tube, guarantee the test tube and follow the stability of centrifugal rotor plate 23 at the rotation in-process.
The working principle of the embodiment is as follows;
(1): preparing centrifugation, placing the prepared gene tissue slices into a test tube, placing the test tube into a placing sleeve 24, pushing the drawing plate 21 to a working position, and covering a door plate;
(2): performing centrifugal operation, namely positioning the test tube in the placing sleeve 24, controlling the centrifugal rotating plate 23 to rotate through the centrifugal motor 22, synchronously driving the test tube to rotate, performing centrifugal treatment on the gene tissue slice through the rotation of the test tube, standing the gene tissue slice after the centrifugal treatment in the test tube, automatically layering the gene tissue slice after the centrifugal treatment according to different components in the process, and enabling the colors displayed by the gene tissue slice after the centrifugal treatment to be different;
(3): the solutions are collected, and the corresponding piston tube 363 is selected to extract the solutions with different color differences according to the color difference positions corresponding to the extraction holes to collect the solutions, so that the accuracy of the collected gene tissue section solutions can be ensured, and the gene tissue section solutions cannot be mixed with other layers of components.
The embodiments of the present invention are preferred embodiments of the present invention, and the scope of the present invention is not limited by these embodiments, so: all equivalent changes made according to the structure, shape and principle of the invention are covered by the protection scope of the invention.

Claims (8)

1. A detector for collecting gene tissue slices layer by layer in a centrifugal mode comprises a detection box body (1) and is characterized in that a centrifugal processing mechanism (2) is arranged inside the detection box body (1), a separation and extraction mechanism (3) is arranged on the detection box body (1), and the separation and extraction mechanism (3) and the centrifugal processing mechanism (2) are matched with each other to collect gene tissue slices subjected to centrifugal layering;
the centrifugal processing mechanism (2) comprises a drawing plate (21), a centrifugal motor (22), a centrifugal rotating plate (23) and a placing sleeve (24), a limiting sliding groove is formed in the bottom of the detection box body (1), the drawing plate (21) is arranged in the limiting sliding groove in a sliding mode, the centrifugal motor (22) is installed on the drawing plate (21) through a motor base, the centrifugal rotating plate (23) is installed on an output shaft of the centrifugal motor (22), and the placing sleeve (24) used for placing test tubes is installed on the centrifugal rotating plate (23);
separation extraction mechanism (3) are including lift cylinder (31), crane (32), extraction pipe (33), butt ring (34), rubber buffer (35) and extraction branch chain (36), install lift cylinder (31) on the inner wall of detection box (1), install crane (32) on lift cylinder (31), install the lower extreme of crane (32) extraction pipe (33), install butt ring (34) through the bearing on the outer wall of extraction pipe (33), install on the inner wall of butt ring (34) and be used for blockking up test tube open-ended rubber buffer (35), be provided with in extraction pipe (33) extraction branch chain (36), evenly seted up on crane (32) with extraction branch chain (36) complex lift groove.
2. The detector as claimed in claim 1, wherein the upper end surface of the placing sleeve (24) is provided with an annular groove, the inner wall of the annular groove is uniformly provided with supporting springs, and the supporting springs are provided with annular plates (241).
3. The detector of claim 2, wherein the placing sleeve (24) is provided with a locking branch chain for positioning the test tube, the locking branch chain comprises a locking mounting block (242), a locking spring (243), a rubber block, a connecting rod (244) and a sleeve ring (245), the placing sleeve (24) is provided with a locking groove along the circumferential direction, the locking mounting block (242) is slidably arranged in the locking groove, the locking spring (243) is arranged between the locking mounting block (242) and the outer wall of the placing sleeve (24), and the rubber block is arranged on the locking mounting block (242);
the placing sleeve (24) is evenly provided with a linkage groove communicated with the annular groove, the connecting rod (244) is arranged in the linkage groove in a sliding mode, one end of the connecting rod (244) is connected to the annular plate (241), and the other end of the connecting rod (244) is installed on the inner wall of the lantern ring (245).
4. The detector as claimed in claim 3, wherein the diameter of the collar (245) increases from top to bottom, and the locking mounting block (242) abuts against the inner wall of the collar (245).
5. The detector as claimed in claim 2, wherein the placing sleeve (24) is provided with drawing holes from top to bottom, the drawing holes are arranged on the placing sleeve (24) in a spiral track, and the placing sleeve (24) is provided with a sliding groove matched with the drawing holes.
6. The detector of claim 5, wherein the extraction branch chain (36) comprises a flexible tube (361), a connecting ring (362), a piston tube (363), a closing plate (364), a connecting frame (365), a closing spring (366) and a control component (367), the connecting ring (362) is uniformly arranged on the lifting frame (32), one end of the flexible tube (361) is connected with the extraction hole, the other end of the flexible tube (361) is connected with the connecting ring (362), the control component (367) is arranged on the outer wall of the lifting frame (32), the piston tube (363) is placed on the control component (367), the nozzle of the piston tube (363) is inserted into the connecting ring (362), the closing plate (364) is slidably arranged in the sliding groove, and the closing spring (366) is arranged between the closing plate (364) and the inner wall of the sliding groove, the closing plate (364) is provided with a connecting frame (365), and the connecting frame (365) is connected with the lifting groove in a sliding mode.
7. The genetic tissue slice centrifugal layer-by-layer collection detector according to claim 6, wherein the control component (367) comprises a support bracket (3671), a moving block (3672) and a control bracket (3673), the support bracket (3671) is arranged on the lifting frame (32) at equal intervals, the support bracket (3671) is used for placing a piston tube (363), a moving groove is formed in the support bracket (3671), the moving block (3672) is arranged in the moving groove in a sliding mode, an arc groove is formed in the moving block (3672), a piston rod on the piston tube (363) is placed in the arc groove, the control bracket (3673) is arranged at the lower end of the moving block (3672), and the control bracket (3673) extends into the lifting groove and abuts against the upper end face of the connecting frame (365).
8. The detector according to claim 7, wherein a rectangular groove for observing the test tube is formed at the right end of the placing sleeve (24), an observation opening is formed in the right end face of the detection box body (1), a glass panel is arranged at the observation opening, a door plate is hinged to the front side of the detection box body (1), a metal strip is arranged on the door plate, and a magnet strip matched with the metal strip is arranged on the detection box body (1).
CN202210619535.1A 2022-06-02 2022-06-02 Detector is collected to gene tissue section centrifugation successive layer Active CN114700188B (en)

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