CN114698840A - Natural plant extract for enhancing immunity and production process thereof - Google Patents

Abstract

The invention discloses a natural plant extract for enhancing immunity and a production process thereof, belonging to the field of development and utilization of natural products and comprising the following raw materials in parts by weight: 15-22 parts of honeysuckle extract, 8-15 parts of medlar extract, 6-12 parts of lucid ganoderma extract, 5-10 parts of ginseng extract, 5-10 parts of astragalus extract, 9-14 parts of mushroom extract, 8-14 parts of tomato extract, 6-12 parts of carrot extract, 3-8 parts of aloe extract and 2-6 parts of green tea extract. The natural plant extract has the effects of regulating endocrine, naturally clearing, providing vitamins, minerals and other special nutrients, and can strengthen, protect and nourish the immune system and effectively strengthen the immunity.

Description

Natural plant extract for enhancing immunity and production process thereof

Technical Field

The invention relates to the field of natural product development and utilization, in particular to a natural plant extract for enhancing immunity and a production process thereof.

Background

Immunity refers to the ability of the body to resist external attack and maintain the stability of the environment in the body. The air is filled with various microorganisms, bacteria, viruses, mycoplasma, chlamydia, fungi, etc. In the case of insufficient immunity, they can both become pathogens of the catch-up shift.

With the rapid development of social economy and frequent occurrence of food safety accidents, the food safety is more and more concerned by the public. Animal husbandry is an important link for solving the problem of food safety, and is directly related to the physical health of people. At present, under the influence of multiple factors such as breeding environment, breeding method, weather, diseases and the like, the subhealth state of the livestock and poultry organism becomes a common phenomenon, which is mainly manifested by low immunity, poor digestion and absorption capacity, reduced vitality and the like.

In order to prevent and treat animal diseases and obtain greater economic benefits, some enterprises often use excessive antibiotics to ensure the health of animals in the animal breeding process. However, an excessive amount of antibiotics leads to a decrease in the quality of meat products from animals and an increase in the resistance to antibiotics and can be transmitted through the food chain to human health posing a serious threat. In view of the side effects of antibiotic use and the growing concern of food safety, research for finding antibiotic substitutes is imperative. The plant extract is natural material from nature, has abundant resource, low cost, and easy acquisition, has antiviral, antiinflammatory, and immunity regulating effects, and has no toxic substance residue. Therefore, the natural plant extract has wide application prospect, and it is particularly important to find substances which can be extracted from natural plants and used for enhancing immunity.

Disclosure of Invention

The invention aims to provide a natural plant extract for enhancing immunity and a production process thereof, which are used for solving the problems in the prior art, can effectively enhance the immunity, and have good efficacy and no toxic or side effect.

In order to achieve the purpose, the invention provides the following scheme:

the invention provides a natural plant extract for enhancing immunity, which comprises the following raw materials in parts by weight: 15-22 parts of honeysuckle extract, 8-15 parts of medlar extract, 6-12 parts of lucid ganoderma extract, 5-10 parts of ginseng extract, 5-10 parts of astragalus extract, 9-14 parts of mushroom extract, 8-14 parts of tomato extract, 6-12 parts of carrot extract, 3-8 parts of aloe extract and 2-6 parts of green tea extract.

Further, the weight parts of the raw materials are as follows: 17-20 parts of honeysuckle extract, 10-13 parts of medlar extract, 8-10 parts of lucid ganoderma extract, 6-8 parts of ginseng extract, 7-9 parts of astragalus extract, 10-12 parts of mushroom extract, 9-12 parts of tomato extract, 8-10 parts of carrot extract, 5-7 parts of aloe extract and 3-5 parts of green tea extract.

The invention also provides a production process of the natural plant extract for immunopotentiation, which comprises the following steps:

(1) respectively preparing the raw materials, cleaning, drying and crushing to obtain powder;

(2) respectively weighing the powder prepared in the step (1), adding water, uniformly mixing, leaching, filtering and collecting an extracting solution;

(3) respectively adding water into the filter residues obtained in the step (2) for extraction, filtering, and combining the two extracting solutions;

(4) adding ethanol into the extracting solution obtained in the step (3), standing, filtering, precipitating, concentrating and drying;

(5) mixing the extracts according to the weight part ratio.

Further, the raw materials in the step (1) are crushed to 20-60 meshes.

Furthermore, the water adding amount in the step (2) is 8-10 times of the weight of each raw material.

Further, the leaching in the step (2) is extraction at 40-80 ℃ for 60-90 min.

Furthermore, the water addition amount in the step (3) is 5-8 times of that of each raw material.

Further, the leaching in the step (3) is extraction at 60-100 ℃ for 40-60 min.

Further, the adding amount of the ethanol in the step (4) is 20-30% of the weight of the extracting solution;

preferably, the ethanol mass fraction is 60-70% ethanol solution.

Further, the standing in the step (4) is carried out for 12-24h at 4-8 ℃;

preferably, the concentration in the step (4) is carried out until the relative density is 1.01-1.05.

The invention discloses the following technical effects:

the chlorogenic acid contained in the honeysuckle has the biological activity, and can promote the metabolism of a human body, regulate the functions of the human body and improve the immunity. The medlar can comprehensively enhance the immunity and has good effects on inhibiting the growth of tumors and cell mutation; has health promoting and blood circulation promoting effects. The Ganoderma spore powder can improve cellular immunity and humoral immunity, promote increase of leukocyte, increase immunoglobulin and complement content, induce interferon production, activate natural killer cell and macrophage activity, and enhance immune organ thymus, spleen and liver weight, thereby enhancing resistance of organism to various diseases. The panaxan and ginsenoside in Ginseng radix are active factors for regulating immunity of Ginseng radix. The nutrient substances such as amino acid, polysaccharide and the like contained in the astragalus can help to enhance the immunity and resist the invasion of pathogenic bacteria. Lentinan in Lentinus Edodes can improve phagocytic function of macrophage, promote generation of T lymphocyte, and improve killing activity of T lymphocyte. Tomato contains rich lycopene, is an antioxidant substance similar to beta-carotene, and has the function of enhancing human body immunity. The carrots contain rich beta-carotene, the carotene is converted into vitamin A, the immunity of the body can be enhanced, and the carrot has an important function in the process of preventing epithelial cell canceration. The lignin in carrot can also improve the immune mechanism of the organism and indirectly kill cancer cells. The asparagus juice can promote the transformation and proliferation of peripheral blood T lymphocytes and is a biological regulator of the organism immune function. Aloe polysaccharide has resistance to any pathogenic nest in vivo, so as to improve self strength to resist pathogenic bacteria and accelerate therapeutic effect. The green tea is helpful for preventing and resisting cancer, and the tea polyphenol can block the synthesis of various carcinogenic substances such as ammonium nitrite in vivo, and has the effects of directly killing cancer cells and improving the immunity of the human body.

The plant capable of enhancing immunity has three functions: firstly, endocrine function is regulated, so that immunity is enhanced; secondly, the medicine has natural clearing efficacy, can clear harmful substances submerged in the organism and protect the immune system; and thirdly, vitamins, minerals and other special nutrients are provided to nourish the immune system. The multiple plant extracts can singly or synergistically play a role in enhancing immunity, and the effect is good.

The preparation method of the invention has simple and convenient operation, high yield of extracted active ingredients, high extraction rate, low water content and capability of fully utilizing raw material resources, and is very suitable for industrial production, and the effective ingredients of plants are reserved.

Detailed Description

Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention.

It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Further, for numerical ranges in this disclosure, it is understood that each intervening value, between the upper and lower limit of that range, is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.

It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The specification and examples are exemplary only.

As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including, but not limited to.

The "parts" in the present invention are all parts by mass unless otherwise specified.

Example 1

A natural plant extract for immunopotentiation comprises the following raw materials in parts by weight: 15 parts of honeysuckle extract, 8 parts of medlar extract, 6 parts of lucid ganoderma extract, 5 parts of ginseng extract, 5 parts of astragalus extract, 9 parts of mushroom extract, 8 parts of tomato extract, 6 parts of carrot extract, 3 parts of aloe extract and 2 parts of green tea extract.

Example 2

The differences from the embodiment 1 are that 22 parts of honeysuckle extract, 15 parts of medlar extract, 12 parts of lucid ganoderma extract, 10 parts of ginseng extract, 10 parts of astragalus extract, 14 parts of mushroom extract, 14 parts of tomato extract, 12 parts of carrot extract, 8 parts of aloe extract and 6 parts of green tea extract.

Example 3

The differences from the embodiment 1 are that the honeysuckle flower extract is 17 parts, the medlar extract is 10 parts, the lucid ganoderma extract is 8 parts, the ginseng extract is 6 parts, the astragalus extract is 7 parts, the mushroom extract is 10 parts, the tomato extract is 9 parts, the carrot extract is 8 parts, the aloe extract is 5 parts, and the green tea extract is 3 parts.

Example 4

The differences from the embodiment 1 are that 20 parts of honeysuckle extract, 13 parts of medlar extract, 10 parts of lucid ganoderma extract, 8 parts of ginseng extract, 9 parts of astragalus extract, 12 parts of mushroom extract, 12 parts of tomato extract, 10 parts of carrot extract, 7 parts of aloe extract and 5 parts of green tea extract.

Example 5

The differences from the embodiment 1 are that the honeysuckle extract is 19 parts, the medlar extract is 12 parts, the lucid ganoderma extract is 9 parts, the ginseng extract is 7 parts, the astragalus extract is 8 parts, the mushroom extract is 11 parts, the tomato extract is 11 parts, the carrot extract is 9 parts, the aloe extract is 6 parts, and the green tea extract is 4 parts.

Example 6

The process for producing natural plant extracts for immunopotentiation according to the above examples 1 to 5 comprises the following steps:

(1) respectively preparing raw materials, cleaning, drying, and pulverizing to 20 meshes to obtain powder;

(2) respectively weighing the powder prepared in the step (1), adding water with the weight 8 times that of the weighed powder, uniformly mixing, leaching for 90min at 40 ℃, filtering, and collecting an extracting solution;

(3) adding water into the filter residue obtained in the step (2) respectively, wherein the water addition amount is 5 times of the weight of the filter residue, leaching for 60min at 60 ℃, filtering, and combining the two extracting solutions;

(4) adding ethanol with the mass fraction of 60% into the extracting solution obtained in the step (3) respectively, wherein the adding amount is 20% of the weight of the extracting solution, standing for 12h at 4 ℃, filtering, precipitating, concentrating and drying until the relative density is 1.01;

(5) mixing the extracts according to the weight part ratio.

Example 7

The difference from example 6 is that each raw material was pulverized to 60 mesh in step (1).

Example 8

The difference from example 6 is that in step (2), water was added in an amount of 10 times the weight of each raw material.

Example 9

The difference from example 6 is that the leaching in step (2) was carried out at 80 ℃ for 60 min.

Example 10

The difference from example 6 is that the amount of water added in step (3) was 8 times as much as the amount of each raw material.

Example 11

The difference from example 6 is that the leaching in step (3) is an extraction at 100 ℃ for 40 min.

Example 12

The difference from example 6 is that the amount of ethanol added in step (4) is 30% by weight of the extract.

Example 13

The difference from example 6 is that the mass fraction of the ethanol solution is 70%.

Example 14

The difference from example 6 is that the standing in step (4) was carried out at 8 ℃ for 24 hours.

Example 15

The difference from example 6 is that the concentration in step (4) was carried out to a relative density of 1.05.

Comparative example 1

The differences from the embodiment 1 are that 8 parts of honeysuckle extract, 4 parts of medlar extract, 5 parts of lucid ganoderma extract, 2 parts of ginseng extract, 3 parts of astragalus extract, 15 parts of mushroom extract, 17 parts of tomato extract, 15 parts of carrot extract, 10 parts of aloe extract and 10 parts of green tea extract. The preparation method is the same as example 1.

Comparative example 2

The method is different from the production process of the natural plant extract for enhancing immunity in example 1, and comprises the following steps:

(1) respectively preparing raw materials, cleaning, drying, and pulverizing to 100 meshes to obtain powder;

(2) respectively weighing the powder prepared in the step (1), adding water 15 times the weight of the weighed powder, uniformly mixing, leaching for 20min at 100 ℃, filtering, and collecting an extracting solution;

(3) adding water into the filter residue obtained in the step (2) respectively, wherein the water addition amount is 10 times of the weight of the filter residue, leaching for 90min at 120 ℃, filtering, and combining the two extracting solutions;

(4) respectively adding 30% by weight of ethanol into the extracting solution obtained in the step (3), standing at room temperature for 12h, filtering, precipitating, concentrating, and drying to relative density of 1.80;

(5) mixing the extracts according to the weight part ratio.

Comparative example 3

The difference from the example 1 is that the honeysuckle extract and the astragalus extract are not contained. The preparation method is the same as example 1.

Effect verification

90 mice were taken and weighed 18-24g, and randomly divided into 9 groups of 10, 8 groups of 10 mice were fed with the natural plant extracts prepared in examples 1-5 and comparative examples 1-3, respectively, 1.5g/kg of mice were given distilled water in an equal amount per day, 1 group of control group was given distilled water once per day for 30 consecutive days, 2% SRBC was intraperitoneally injected on the 25 th day after immunization for 5 days, the spleen was sacrificed by dislocation, the spleen was taken out, filtered through a 200-mesh screen, washed 3 times with Hanks's solution, centrifuged at 100Or/min for 10min each time, and the cells were suspended in 5mLRPM1640 culture solution. Heating and dissolving the surface layer culture medium, preserving heat in a 45 ℃ water bath, mixing with an equivalent amount of 2-fold concentration Hanks solution, subpackaging into small test tubes, adding 0.5mL of each tube, adding 50 mu L of 110% SRBC and 20 mu L of spleen cell suspension, uniformly mixing, inverting, culturing in a carbon dioxide incubator for 1 hour, adding complement (1:10) diluted by SA buffer solution, continuing to culture for 1 hour, and counting the number of hemolytic plaques, wherein the results are shown in Table 1.

TABLE 1 Effect of the invention on mouse antibody-producing cells: (

n＝10)

As can be seen from Table 1, the natural plant extracts (examples 1-5) prepared by the invention can obviously increase antibody-producing cells of mice, and the effect is obviously better than that of comparative example 1 (different raw material ratios) and comparative example 2 (different plant extract production processes) and comparative example 3 (no honeysuckle extract and astragalus extract are added in the raw materials), especially the best effect of example 5, so that the raw material ratios, the plant extract production processes and the raw material components have influence on the curative effect of the prepared product, therefore, the invention adopts the specific raw material ratios, the plant extract production processes and the selection of the proper raw material components, and the prepared plant extracts have the effect of improving antibody-producing cells.

The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.

Claims (10)

1. A natural plant extract for immunopotentiation characterized by: the feed comprises the following raw materials in parts by weight: 15-22 parts of honeysuckle extract, 8-15 parts of medlar extract, 6-12 parts of lucid ganoderma extract, 5-10 parts of ginseng extract, 5-10 parts of astragalus extract, 9-14 parts of mushroom extract, 8-14 parts of tomato extract, 6-12 parts of carrot extract, 3-8 parts of aloe extract and 2-6 parts of green tea extract.

2. A natural plant extract for immunopotentiation according to claim 1, wherein: the weight parts of the raw materials are as follows: 17-20 parts of honeysuckle extract, 10-13 parts of medlar extract, 8-10 parts of lucid ganoderma extract, 6-8 parts of ginseng extract, 7-9 parts of astragalus extract, 10-12 parts of mushroom extract, 9-12 parts of tomato extract, 8-10 parts of carrot extract, 5-7 parts of aloe extract and 3-5 parts of green tea extract.

3. A process for the production of a natural plant extract for immunopotentiation according to any one of claims 1-2, wherein: the method comprises the following steps:

(1) respectively preparing the raw materials, cleaning, drying and crushing to obtain powder;

(2) respectively weighing the powder prepared in the step (1), adding water, uniformly mixing, leaching, filtering and collecting an extracting solution;

(3) respectively adding water into the filter residues obtained in the step (2) for extraction, filtering, and combining the two extracting solutions;

(4) adding ethanol into the extracting solution obtained in the step (3), standing, filtering, precipitating, concentrating and drying;

(5) mixing the extracts according to the weight part ratio.

4. The production process according to claim 3, characterized in that: and (2) crushing the raw materials in the step (1) to 20-60 meshes.

5. The production process according to claim 3, characterized in that: the water adding amount in the step (2) is 8-10 times of the weight of each raw material.

6. The production process according to claim 3, characterized in that: the leaching in the step (2) is carried out for 60-90min at 40-80 ℃.

7. The production process according to claim 3, characterized in that: the water addition amount in the step (3) is 5-8 times of that of each raw material.

8. The production process according to claim 3, characterized in that: the leaching in the step (3) is carried out for 40-60min at 60-100 ℃.

9. The production process according to claim 3, characterized in that: the adding amount of the ethanol in the step (4) is 20-30% of the weight of the extracting solution;

preferably, the ethanol mass fraction is 60-70% ethanol solution.

10. The production process according to claim 3, wherein the standing in the step (4) is standing at 4 to 8 ℃ for 12 to 24 hours;

preferably, the concentration in the step (4) is carried out until the relative density is 1.01-1.05.