CN114685213A - Preparation method of organic compound fertilizer for peach branches - Google Patents

Preparation method of organic compound fertilizer for peach branches Download PDF

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Publication number
CN114685213A
CN114685213A CN202011608204.5A CN202011608204A CN114685213A CN 114685213 A CN114685213 A CN 114685213A CN 202011608204 A CN202011608204 A CN 202011608204A CN 114685213 A CN114685213 A CN 114685213A
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liquid
culture medium
temperature
content
fermentation
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高程达
梁军
芮朝利
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Beijing Reclaimed Water Huafeng Biology Technology Co ltd
Beijing University of Agriculture
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Beijing Reclaimed Water Huafeng Biology Technology Co ltd
Beijing University of Agriculture
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F3/00—Fertilisers from human or animal excrements, e.g. manure
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The invention relates to a preparation method of an organic compound fertilizer for peach branches, which comprises the steps of crushing peach branches into peach branch powder; adding water and organic substances into the broken peach branch powderCarrying out compost fermentation on the fertilizer and the compound degrading microbial inoculum at the environmental temperature of 19-35 ℃ for 35-45 days, and then aging for 45 days at normal temperature and normal pressure; wherein the composite degrading bacteria agent comprises composite degrading bacteria liquid and an adsorbent, wherein the composite degrading bacteria liquid contains 3.60 multiplied by 10 strains9The content of the bacillus subtilis bacterial liquid/gram is more than 3.60 multiplied by 109The strain content of the yeast bacterial liquid is 2.40 multiplied by 10 per gram9The strain content of the rhodopseudomonas palustris strain liquid is more than 1.80 multiplied by 109The content of azotobacteria bacterial liquid and bacterial strain is 3.60X 109More than one gram of trichoderma bacteria liquid and total bacteria number in composite degrading bacteria agent>4.5×109Per gram. The fertilizer prepared by the invention has the characteristics of quick temperature rise, long high-temperature period, high humus content and low lignocellulose content.

Description

Preparation method of organic compound fertilizer for peach branches
Technical Field
The invention relates to the technical field of agricultural biology and science, in particular to a preparation method of an organic compound fertilizer.
Background
The yield of the waste branches of crops is more than 20 million tons every year in the world, and China accounts for 5 million tons. Taking the flat valley as a famous peach producing base as an example, the average annual production of rejected waste peach branches is about 15 million tons or more. Because the peach branches have high lignin content, large molecular weight and complex structure, the natural degradation rate of the peach branches is very slow, and the peach branches have little influence on the regional environment. In addition, the process of obtaining heat through incineration is slow in progress and low in utilization rate, and environmental pollution is easily caused, so that a large number of peach branches are disorderly discarded and stacked, land resources are occupied, and meanwhile, natural resources are wasted.
The peach branch fermentation compost is an effective way for solving the problems. However, most of composting microbial agents and composting methods used at the present stage are dedicated to waste crops of straws, and peach branches have high lignification degree, high lignin content and higher decomposition difficulty relative to the straw crops, so that the problems cannot be fundamentally solved if the decomposition rate is not increased.
Disclosure of Invention
The present invention has been made to solve one or more of the above problems, and an object of the present invention is to provide a method for preparing an organic complex fertilizer capable of accelerating the transformation of peach branches.
A preparation method of the peach branch organic compound fertilizer comprises the following steps: crushing peach branches into peach branch powder; adding water, organic fertilizer and composite degrading bacteria agent into the peach branch powder to perform composting fermentation at the temperature of 19-35 ℃ for 35-45 days, and then standing and aging for 45 days at normal temperature and normal pressure.
Wherein the composite degrading bacteria agent comprises composite degrading bacteria liquid and an adsorbent, wherein the composite degrading bacteria liquid contains 3.60 multiplied by 10 strains9The content of the bacillus subtilis bacterial liquid/gram is more than 3.60 multiplied by 109The strain content of the yeast bacterial liquid is 2.40 multiplied by 10 per gram9The strain content of the rhodopseudomonas palustris strain liquid is more than 1.80 multiplied by 109The content of azotobacteria bacterial liquid and bacterial strain is 3.60X 109More than one gram of trichoderma liquid, and the total bacterial number in the composite degrading bacterial agent>4.5×109/g。
The mass ratio of the composite degrading bacteria liquid to the adsorbent is 1:2, the mass ratio of the peach branch powder, the water, the organic fertilizer and the composite degrading bacteria agent is 3.75-5: 3.75-5: 2.5: 0.01.
wherein, the step of compost fermentation comprises: under natural conditions, stacking raw materials consisting of peach branch powder, water, organic fertilizer and composite degradation microbial inoculum into a strip pile, and performing natural composting fermentation, wherein the size of the strip pile is 8-12 meters long, 2-3 meters wide and 1-1.5 meters high.
In the compost fermentation process, the environmental temperature and the temperature of a compost are recorded simultaneously from the feeding day, the temperature is measured in the morning every day, when the temperature of the compost reaches 60 ℃, the compost is turned for the first time, then the temperature and the water of the compost are measured in the morning, in the middle and at night every day, the compost is turned once when the temperature of the compost exceeds 60 ℃, the moisture content of the compost is lower than 50%, and the water of the material is supplemented to 60%.
Wherein the length of the peach branch powder is 05-2.5cm, and the organic fertilizer is one or a mixture of pig manure and chicken manure.
The bacillus subtilis liquid is obtained by activating bacillus subtilis and then inoculating the activated bacillus subtilis liquid in a bacillus subtilis culture medium for enrichment, wherein 1L of the bacillus subtilis culture medium comprises 30g of corn flour, 35g of soybean meal, 5g of NaCl and the balance of 1L of distilled water. Wherein the enrichment condition is that air is purified, and oxygen is added to keep dissolved oxygen above 5 PPM; the fermentation temperature was 37 ℃.
Wherein, the yeast liquid is obtained by activating yeast, inoculating the activated yeast in a yeast culture medium, and enriching, wherein 1L of the culture medium comprises 40 g of starch, 15g of agar, and the balance of 1L of distilled water; the enrichment condition comprises fermentation temperature of 25-28 deg.C, pH of 4-6, air purification, and oxygen enrichment to maintain dissolved oxygen above 4 PPM.
The trichoderma harzianum liquid is obtained by activating trichoderma harzianum and then inoculating the activated trichoderma harzianum liquid in a mildew culture medium for enrichment, wherein 1L of the culture medium comprises 1L of 20% potato juice, 3g of monopotassium phosphate, 1.5g of magnesium sulfate and 20g of glucose; the enrichment conditions are as follows: keeping the fermentation temperature at 28 deg.C, introducing purified air, and increasing oxygen to maintain dissolved oxygen above 5 PPM.
Wherein, the rhodopseudomonas palustris bacterial liquid is prepared by inoculating activated rhodopseudomonas palustris in a rhodopseudomonas palustris culture medium for enrichment; wherein 1L of rhodopseudomonas palustris culture medium comprises 30g of corn flour, 1g of monopotassium phosphate, 0.5g of magnesium sulfate and the balance of 1L of distilled water; the enrichment conditions are as follows: the fermentation temperature is 30 ℃, and the illumination intensity is 4000 and 5000 lux.
The azotobacteria bacterial liquid is formed by inoculating activated azotobacteria into an azotobacteria culture medium for enrichment; wherein 1L of azotobacter culture medium contains 10g of glucose, 1g of monopotassium phosphate, 0.2g of magnesium sulfate, 0.2g of calcium sulfate and the balance of 1L of distilled water; the enrichment conditions are as follows: introducing purified air, and maintaining the fermentation temperature at 25-28 deg.C.
Compared with the prior art, the invention has the following technical advantages:
1. the fertilizer prepared by the invention has the advantages of fast temperature rise and long high-temperature period, so that the internal microbial population is rich and the fertilizer activity is high. The actual measurement shows that the starting time is 3-4 days, and the high temperature period is 28-25 days.
2. The preparation method disclosed by the invention has high conversion efficiency on lignin and cellulose in the peach branches. Tests prove that the prepared organic compound fertilizer has the lignin content of below 17 percent and the cellulose content of below 7 percent.
3. The fertilizer prepared by the invention has high nutrient content and comprehensive nutrition. Wherein the organic matter content is more than 48.13%, the humus content is more than 21.9%, and the contents of quick-acting phosphorus and quick-acting potassium (which can be directly absorbed by plants) are also obviously increased.
4. Various indexes of the peach branch organic compound fertilizer prepared by the invention meet the national standards (NY525-2012 and NY 884-2012).
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts. In the drawings:
fig. 1 is a graph showing a comparison of fermentation temperature changes of peach branch organic complex fertilizer according to an embodiment of the present invention.
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
Exemplary embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings. While exemplary embodiments of the present disclosure are shown in the drawings, it should be understood that the present disclosure may be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art.
The basic idea of the invention is that peach branches are used as raw materials to carry out fermentation composting by using a targeted microbial compound degradation microbial inoculum, lignin and cellulose in the peach branches are fully converted into sugar, the growth of microbes is promoted, and the prepared fertilizer is richer in microbial inoculum and high in nutrition content.
In the present invention, the original sources of the species used are as follows:
bacillus subtilis purchased from Beijing Baina union of Industrial science and biotechnology research institute and deposited in Beijing biological microorganism strain collection center with the collection number BNCC 193111.
Yeast (Saccharomyces), purchased from research institute of Biotechnology, Baina, Beijing, and deposited in Beijing biological microorganism culture Collection with the deposit number BNCC 336054.
The rhodopseudomonas palustris is purchased from Beijing Baina Chuanglian Biotechnology research institute and is preserved in Beijing biological microorganism culture collection center, and the preservation number is BNCC 336448.
The Azotobacter (Azotobacter sp.) used was purchased from the institute of Biotechnology, Baina, Beijing and collected in the Beijing biological microorganism culture Collection with the collection number BNCC 335805.
The Trichoderma (Trichoderma spp.) used was purchased from the institute of Biotechnology, Baina, Beijing and collected in the Beina biological microorganism culture Collection with the collection number BNCC 189286.
The technical solution of the present invention will be explained in further detail by the form of specific embodiments.
Example 1 enrichment of the Medium
1. The bacillus subtilis culture medium comprises the following components:
the 1L culture medium contains corn flour 30g, soybean meal 35g, NaCl 5g, and the balance of 1L distilled water.
2. The yeast culture medium comprises the following components:
the yeast culture medium comprises the following components: 1L of the culture medium contains 40 g of starch, 15g of agar and the balance of 1L of distilled water.
3. The components of the rhodopseudomonas palustris culture medium are as follows:
the rhodopseudomonas palustris culture medium comprises the following components: the 1L culture medium contains corn flour 30g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g, and the rest is 1L distilled water.
4. The nitrogen-fixing bacteria culture medium comprises the following components:
the nitrogen-fixing bacteria culture medium comprises the following components: 1L of culture medium contains 10g of glucose, 1g of monopotassium phosphate, 0.2g of magnesium sulfate, 0.2g of calcium sulfate and the balance of 1L of distilled water.
5. The components of the trichoderma culture medium are as follows:
the trichoderma culture medium comprises the following components: the 1L culture medium comprises 1L of 20% potato juice, 3g of potassium dihydrogen phosphate, 1.5g of magnesium sulfate and 20g of glucose.
Example 2 enrichment Process
1. The enrichment process of the bacillus subtilis comprises the following steps: and unfreezing the purchased bacillus subtilis microbial inoculum, inoculating the bacillus subtilis microbial inoculum into a screening culture medium for culture, selecting a thriving colony from the bacillus subtilis microbial inoculum, streaking and inoculating the selected colony again, and repeatedly screening for 4-5 times to obtain a well-grown colony. Selecting the same components as the screening culture medium as an enrichment culture medium, selecting a colony with good growth to be inoculated into the enrichment culture medium, and carrying out enrichment fermentation culture. The fermentation culture conditions are that air is purified, and oxygen is added to keep dissolved oxygen above 5 PPM; the fermentation temperature was 37 ℃. Obtaining the bacillus subtilis fermentation liquor.
2. And (3) enriching yeast: and unfreezing the purchased yeast agent, inoculating the thawed yeast agent into a screening culture medium for culture, selecting out a thriving colony from the culture medium, streaking and inoculating the colony again, and repeatedly screening for 4-5 times to obtain a colony with good growth. Selecting the same components as the screening culture medium as an enrichment culture medium, selecting a colony growing well, inoculating into the enrichment culture medium, and performing enrichment fermentation culture. The enrichment fermentation conditions are as follows: fermenting at 25-28 deg.C and pH of 4-6, purifying air, and adding oxygen to maintain dissolved oxygen above 4 PPM. Obtaining the yeast fermentation liquor.
3. Enrichment process of rhodopseudomonas palustris: and unfreezing the purchased rhodopseudomonas palustris microbial inoculum, inoculating the rhodopseudomonas palustris microbial inoculum into a screening culture medium for culture, selecting out a thriving colony from the rhodopseudomonas palustris microbial inoculum, streaking and inoculating the thriving colony again, and repeatedly screening for 4-5 times to obtain a well-grown colony. Selecting the same components as the screening culture medium as an enrichment culture medium, selecting a colony growing well, inoculating into the enrichment culture medium, and performing enrichment fermentation culture. The conditions of enrichment fermentation are as follows: the enrichment fermentation conditions are as follows: the fermentation temperature is 30 ℃, and the illumination intensity is 4000 and 5000 lux. Obtaining the rhodopseudomonas palustris fermentation liquor.
4. And (3) enriching nitrogen-fixing bacteria: and unfreezing the purchased azotobacter inoculum, inoculating the obtained azotobacter inoculum into a screening culture medium for culture, selecting out a growing and robust bacterial colony from the obtained azotobacter inoculum, streaking and inoculating the bacterial colony again, and repeatedly screening for 4-5 times to obtain the bacterial colony with good growth. Selecting the same components as the screening culture medium as an enrichment culture medium, selecting a colony with good growth to be inoculated into the enrichment culture medium, and carrying out enrichment fermentation culture. The conditions of enrichment fermentation are as follows: introducing purified air, and maintaining the fermentation temperature at 25-28 deg.C. Obtaining the azotobacteria fermentation liquor.
5. And (3) enrichment process of trichoderma: and unfreezing the purchased trichoderma fungicide, inoculating the trichoderma fungicide into a screening culture medium for culture, selecting out a thriving colony from the trichoderma fungicide, streaking and inoculating the colony again, and repeatedly screening for 4-5 times to obtain a colony with good growth. Selecting the same components as the screening culture medium as an enrichment culture medium, selecting a colony with good growth to be inoculated into the enrichment culture medium, and carrying out enrichment fermentation culture. The enrichment fermentation conditions are as follows: keeping the fermentation temperature at 28 deg.C, introducing purified air, and increasing oxygen to maintain dissolved oxygen above 5 PPM. Obtaining the trichoderma fermentation liquor.
Example 3 preparation of Complex degrading microbial inoculum S
Respectively diluting and coating the obtained bacillus subtilis fermentation liquor, yeast fermentation liquor, rhodopseudomonas palustris fermentation liquor, azotobacter fermentation liquor and trichoderma fermentation liquor, and selecting bacillus subtilis with the weight of 3.60 multiplied by 109One per gram, yeast 3.60 × 109Rhodopseudomonas palustris 2.40X 1091.80X 10 of azotobacteria9Respectively, Trichoderma 3.60X 109And adding nutrient solution one by one to prepare 1g of composite degrading bacteria liquid.
Sieving the adsorbent with a 50-mesh sieve, and adding the adsorbent and the composite degrading bacteria liquid in a ratio of 2:1 to prepare the solid composite degrading bacteria S. The adsorbent is turf or vermiculite.
The composition of the microbial composite degradation bacterial liquid related to the invention is exemplarily shown in the form of a table below. The microbial composite degraded bacterial liquid shown in the following table does not represent all embodiments of the microbial composite degraded bacterial liquid, and the scope of the claims required for the microbial composite degraded bacterial liquid of the present invention is subject to the final limitation of the claims.
Table 1: 1g of microorganism composite degradation bacterial liquid composition (unit: strain)
Figure BDA0002874052290000061
Example 4 preparation method of organic Compound Fertilizer X
The method specifically comprises the following steps:
pulverizing collected peach branches, and sequentially pulverizing into 0.5-2.5cm peach branch powder by coarse powder machine and fine powder machine. Putting the peach branch scraps into a fermentation device, adding water for swelling, supplementing chicken manure serving as a carbon source, and finally uniformly scattering the composite degrading microbial inoculum prepared in the embodiment 3, wherein the adding ratio of the peach branch scraps, the water, the chicken manure and the composite degrading microbial inoculum is 5:5: 2.5: 0.01. stacking into a stack, and fermenting at ambient temperature (19-35 deg.C) and normal pressure by natural ventilation. Wherein the size of the strip pile is 10 meters long, 2.5 meters wide and 1.2 meters high. Turning over once on the third day of fermentation; turning over the pile once to twice every day on the fourth to fourteenth days of fermentation, replenishing water once on the tenth day of fermentation, turning over the pile once every day on the fifteenth to twenty-fourth days of fermentation, and replenishing water once on twenty-four days. After natural fermentation for 38 days (2019.5.21-2019.6.28), keeping the original environment and continuing aging for 45 days to prepare the organic compound fertilizer X.
The detection shows that the starting time of the organic compound fertilizer X is 3 days (5 months and 24 days) to 60 ℃, the high-temperature period (above 60 ℃) is as long as 34 days (5 months and 24 days to 6 months and 27 days), and the specific result is shown as a curve X in figure 1. In the fertilizer, the content of organic matters is 48.13%, wherein the content of lignin is 16.12%, the content of cellulose is 6.6%, the content of humus is 21.9%, the content of hydrolyzable nitrogen is 2526mg/kg, the content of quick-acting phosphorus is 6083mg/kg, and the content of quick-acting potassium is 9727 mg/kg.
EXAMPLE 5 preparation of organic Compound Fertilizer Y
The method specifically comprises the following steps:
pulverizing collected peach branches, and sequentially pulverizing into 0.5-2.0cm of peach branch powder by coarse powder machine and fine powder machine. Putting the peach branch scraps into a fermentation device, adding water for swelling, supplementing pig manure serving as a carbon source, and finally uniformly scattering the composite degrading microbial inoculum prepared in the example 3, wherein the adding proportion of the peach branch scraps, the water, the pig manure and the composite degrading microbial inoculum is as follows. 3.75:3.75: 2.5: 0.01. stacking into a strip pile, and fermenting naturally in a ventilation way at ambient temperature (19-35 ℃) and normal pressure. Wherein the size of the strip pile is 8 meters long, 2 meters wide and 1 meter high. Turning over once on the third day of fermentation; turning over the pile once to twice every day on the fourth to fourteenth days of fermentation, replenishing water once on the tenth day of fermentation, turning over the pile once every day on the fifteenth to twenty-fourth days of fermentation, and replenishing water once on twenty-four days. And after fermenting for 35 days, continuously aging for 45 days to obtain the organic compound fertilizer Y.
The detection shows that the starting temperature of the organic compound fertilizer Y reaches 60 ℃ in 3 days, and the high temperature period (above 60 ℃) reaches 32 days. In the fertilizer, the content of organic matters is 50.09 percent, wherein the content of lignin is 16.98 percent, the content of cellulose is 6.8 percent, the content of humus is 23 percent, the content of hydrolyzable nitrogen is 2257mg/kg, the content of quick-acting phosphorus is 5974mg/kg, and the content of quick-acting potassium is 9523 mg/kg.
EXAMPLE 6 preparation of organic Compound Fertilizer Z
The method specifically comprises the following steps:
pulverizing collected peach branches, and sequentially pulverizing into 1.5-2.5cm peach branch powder by coarse powder machine and fine powder machine. Putting the peach branch scraps into a fermentation device, adding water for swelling, supplementing chicken manure serving as a carbon source, and finally uniformly scattering the composite degrading microbial inoculum prepared in the embodiment 3, wherein the adding ratio of the peach branch scraps, the water, the chicken manure and the composite degrading microbial inoculum is 3.75: 2.5: 0.01. stacking into a strip pile, and fermenting naturally in a ventilation way at ambient temperature (19-35 ℃) and normal pressure. Wherein the size of the strip pile is 12 meters long, 3 meters wide and 1.5 meters high. Turning over once on the third day of fermentation; turning over the pile once to twice every day on the fourth to fourteenth days of fermentation, replenishing water once on the tenth day of fermentation, turning over the pile once every day on the fifteenth to twenty-fourth days of fermentation, and replenishing water once on twenty-four days. And after fermenting for 45 days, aging for 45 days to obtain the organic compound fertilizer Z.
The detection shows that the starting temperature of the organic compound fertilizer Z reaches 60 ℃ in 2.5 days, and the high temperature period (above 60 ℃) reaches 35 days. In the fertilizer, the content of organic matters is 48.13 percent, wherein the content of lignin is 15.75 percent, the content of cellulose is 6.1 percent, the content of humus is 22.2 percent, the content of hydrolyzable nitrogen is 2742mg/kg, the content of quick-acting phosphorus is 6123mg/kg, and the content of quick-acting potassium is 9854 mg/kg.
Test examples comparison of quality of various organic Compound fertilizers
Keeping the operation steps of compost fermentation the same as other added components, selecting organic compound fertilizer X1 without adding any microbial compound degradation microbial inoculum as a blank control, adding organic compound fertilizer X2 produced by core flora with the same content of compound degradation microbial inoculum S, adding organic compound fertilizer X3 produced by auxiliary flora with the same content of compound degradation microbial inoculum S, and comparing the quality of the organic compound fertilizer X4 with the same amount of straw degradation microbial inoculum M with the quality of the organic compound fertilizer X recorded in the example 4. Wherein the straw degrading microbial inoculum M is a straw returning decomposing agent produced by MicroBiotechnology Limited company in Guangzhou city. The comparative results are as follows:
the results of comparing the fermentation temperatures during the fertilizer production process are shown in FIG. 1. As can be seen from FIG. 1, the organic compound fertilizer X has the fastest temperature rise and the longest high-temperature period, which indicates that the microorganisms in the organic compound fertilizer X are the most active and the nutrient content is richer.
The lignin and cellulose contents of the fertilizers were compared and the results are shown in tables 1 and 2 below. As can be seen from tables 1 and 2, the contents of lignin and cellulose in the organic compound fertilizer X are both lower than those of other fertilizers to a greater extent, which shows that the microbial compound degradation microbial inoculum S used in the fertilizer has better lignin and cellulose degradation capability on peach branches under the same fermentation conditions.
Table 1: determination of Lignin content (%)
2019, 5 months and 19 days 6 and 28 months in 2019 7 month and 28 days 2019
Organic compound fertilizer X1 29.72 28.68 28.54
Organic compound fertilizer X2 28.97 26.14 26.01
Organic compound fertilizer X3 29.25 27.37 26.52
Organic compound fertilizer X4 28.70 26.44 26.11
Organic compound fertilizer X 29.58 16.12 15.44
Table 2: cellulose content determination (%)
2019, 5 months and 19 days 6 and 28 months in 2019 7 month and 28 days 2019
Organic compound fertilizer X1 13.2 12.9 12.7
Organic compound fertilizer X2 13.5 12.1 11.7
Organic compound fertilizer X3 12.7 11.9 11.5
Organic compound fertilizer X4 13.2 8.1 7.9
Organic compound fertilizer X 12.9 6.6 6.4
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.

Claims (9)

1. The preparation method of the peach branch organic compound fertilizer is characterized by comprising the following steps:
crushing peach branches into peach branch powder;
adding water, organic fertilizer and composite degradation microbial inoculum into the peach branch powder for composting fermentation, wherein the fermentation environment temperature is 19-35 ℃, and the fermentation time is 35-45 days;
then standing and aging for 45 days at normal temperature and normal pressure;
wherein the composite degrading bacteria agent comprises composite degrading bacteria liquid and an adsorbent, wherein the composite degrading bacteria liquid contains 3.60 multiplied by 10 strains9The content of the bacillus subtilis bacterial liquid/gram is more than 3.60 multiplied by 109The strain content of the yeast bacterial liquid is 2.40 multiplied by 10 per gram9The strain content of the rhodopseudomonas palustris bacterial liquid is more than 1.80 multiplied by 109The content of azotobacteria bacterial liquid and bacterial strain is 3.60X 109More than one gram of trichoderma liquid, and the total bacterial number in the composite degrading bacterial agent>4.5×109Per gram.
2. The method according to claim 1, wherein the reaction mixture,
the mass ratio of the composite degrading bacteria liquid to the adsorbent is 1:2, and the mass ratio of the peach branch powder, the water, the organic fertilizer and the composite degrading bacteria agent is 3.75-5: 3.75-5: 2.5: 0.01.
3. the method of claim 1, wherein the step of fermenting the compost comprises:
under natural conditions, stacking raw materials consisting of the peach branch powder, the water, the organic fertilizer and the composite degrading microbial inoculum into a strip pile, and performing natural composting fermentation, wherein the strip pile is 8-12 meters long, 2-3 meters wide and 1-1.5 meters high.
4. The method according to claim 3,
the method comprises the steps of starting from the feeding day, simultaneously recording the ambient temperature and the temperature of a stack, measuring the temperature in the morning every day, starting to turn the stack for the first time when the temperature of the stack reaches above 60 ℃, measuring the temperature and the water of the stack for the early, middle and late days every day, turning the stack once when the temperature of the stack exceeds 60 ℃, and supplementing the water of the stack to 60% when the water content of the stack is lower than 50%.
5. The method according to claim 1, wherein the reaction mixture,
the length of the peach branch powder is 0.5-2.5cm, and the organic fertilizer is one or a mixture of pig manure and chicken manure.
6. The method according to claim 1, wherein the reaction mixture,
the bacillus subtilis liquid is obtained by activating bacillus subtilis and then inoculating the activated bacillus subtilis liquid into a bacillus subtilis culture medium for enrichment, wherein 1L of the bacillus subtilis culture medium comprises 30g of corn flour, 35g of bean pulp and 5g of NaCl, and the balance is 1L of distilled water;
wherein the enrichment condition is that air is purified, and oxygen is increased to keep dissolved oxygen above 5 PPM; the fermentation temperature was 37 ℃.
7. The method according to claim 1, wherein the reaction mixture,
the yeast liquid is obtained by activating yeast, inoculating the activated yeast in a yeast culture medium, and enriching, wherein 1L of the culture medium comprises 40 g of starch, 15g of agar, and the balance of 1L of distilled water;
the enrichment condition is that the fermentation temperature is 25-28 deg.C, pH is 4-6, air is purified, and oxygen is added to maintain dissolved oxygen above 4 PPM.
8. The method according to claim 1, wherein the reaction mixture,
the trichoderma fungus liquid is obtained by activating trichoderma fungus and then inoculating the activated trichoderma fungus liquid into a fungus culture medium for enrichment, wherein 1L of the culture medium comprises 1L of 20% potato juice, 3g of monopotassium phosphate, 1.5g of magnesium sulfate and 20g of glucose;
the enrichment conditions are as follows: keeping the fermentation temperature at 28 deg.C, introducing purified air, and increasing oxygen to maintain dissolved oxygen above 5 PPM.
9. The method according to claim 1, wherein the reaction mixture,
the rhodopseudomonas palustris bacterial liquid is prepared by inoculating activated rhodopseudomonas palustris in a rhodopseudomonas palustris culture medium for enrichment; wherein 1L of rhodopseudomonas palustris culture medium comprises 30g of corn flour, 1g of monopotassium phosphate, 0.5g of magnesium sulfate and the balance of 1L of distilled water; the enrichment conditions are as follows: the fermentation temperature is 30 ℃, and the illumination intensity is 4000 and 5000 lux.
The azotobacter bacterial liquid is formed by inoculating activated azotobacter in an azotobacter culture medium and enriching; wherein 1L of azotobacter culture medium contains 10g of glucose, 1g of monopotassium phosphate, 0.2g of magnesium sulfate, 0.2g of calcium sulfate and the balance of 1L of distilled water; the enrichment conditions are as follows: introducing purified air, and maintaining the fermentation temperature at 25-28 deg.C.
CN202011608204.5A 2020-12-30 2020-12-30 Preparation method of organic compound fertilizer for peach branches Pending CN114685213A (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106282048A (en) * 2016-08-04 2017-01-04 华侨大学 A kind of complex microorganism preparations and its preparation method and application
CN109136148A (en) * 2018-09-21 2019-01-04 浙江省农业科学院 A kind of high-temperature fibre element degradation bacillus screening technique and application method
CN109679860A (en) * 2018-11-30 2019-04-26 北京国环清华环境工程设计研究院有限公司 A kind of composite bacteria agent and the preparation method and application thereof for the processing of gardens green waste

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106282048A (en) * 2016-08-04 2017-01-04 华侨大学 A kind of complex microorganism preparations and its preparation method and application
CN109136148A (en) * 2018-09-21 2019-01-04 浙江省农业科学院 A kind of high-temperature fibre element degradation bacillus screening technique and application method
CN109679860A (en) * 2018-11-30 2019-04-26 北京国环清华环境工程设计研究院有限公司 A kind of composite bacteria agent and the preparation method and application thereof for the processing of gardens green waste

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