CN114680141A - Use of a natural composition as a disinfectant in blocking the transmission of viruses through packaging surfaces - Google Patents
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Abstract
The invention relates to an application of a natural composition as a disinfectant in blocking virus from spreading through a packaging surface. The natural composition can be used for disinfection in food processing, production, circulation and use links, can also be used for disinfection of surrounding environments of public health places such as hospitals, aviation, public health and the like, and disinfection of parts such as human hands, faces and the like, and has wide market application prospect.
Description
Technical Field
The invention belongs to the field of biological medicine, relates to an application of a natural composition as a disinfectant in blocking virus from being transmitted through a packaging surface, and particularly relates to an application of a natural composition in preventing and killing coronavirus on food or article packaging.
Background
The traditional Chinese medicine considers that: the lung governs respiration and opens into the nose, warms up pathogenic factors and first attacks the lung to prevent epidemic diseases, and most importantly, the spreading path of epidemic qi is cut off to protect the nose, mouth and skin of a human body. The experts in traditional Chinese medicine indicate that: the prevention of Chinese herbs can be expressed in two sentences, one sentence called strengthening body qi and the second sentence called avoiding pathogenic qi. The traditional Chinese medicine has four flavors and five flavors, and the aromatic traditional Chinese medicine can play the roles of avoiding plague, avoiding dirty, refreshing, restoring consciousness, relieving stuffiness and inducing resuscitation, and can prevent infectious diseases such as influenza, for example, pepper and aniseed are common traditional Chinese medicines and also are aromatic famous materials for making dishes.
The research of modern medicine finds that the plant volatile oil is the material basis of aromatic traditional Chinese medicines, and in vitro tests find that the volatile oil has certain bacteriostatic and antiviral effects, is aromatic and avoids dirt, and changes the living environment where viruses attach. For example, shikimic acid, a component in Chinese medicine anise, is a raw material for preparing oseltamivir and is used for developing anti-avian influenza medicines.
Coronaviridae (Coronaviridae) viruses are a large group of viruses that are widely found in nature and infect vertebrates such as humans, mice, pigs, cats, dogs, wolves, chickens, cattle, birds. The human disease that can be caused by the disease is mainly respiratory infection (including severe acute respiratory syndrome, SARS). The virus is very temperature sensitive and grows well at 33 ℃ but is inhibited at 35 ℃. Due to this property, winter and early spring are the epidemic seasons of the viral disease. Coronavirus is one of the main pathogens of common cold of adults, the infection rate of children is high, the infection rate is mainly upper respiratory tract infection, the main symptoms are watery stool, fever and vomiting, even bloody watery stool appears in severe cases, and the nervous system syndrome is caused in rare cases. In 2019, a new coronavirus was shown to infect pneumonia, and the pathogen is a new coronavirus which has not been found in human before, namely the new coronavirus. Based on the current epidemiological investigation and research results, the incubation period is 1 to 14 days, and is mostly 3 to 7 days; the infection source is mainly a patient infected by the novel coronavirus, and asymptomatic infected patients can also become the infection source; the main transmission path is through respiratory droplets and contact transmission, the possibility of transmission through aerosol exists under the condition of long-time exposure to high-concentration aerosol in a relatively closed environment, and other transmission paths are yet to be determined; the general susceptibility of people is changed into a global public health incident at present, the economic development and the people life of each country are seriously influenced, and the world first-size problem in 2020 is solved.
At present, the main focus of epidemic prevention and control in China is to strictly prevent the outward transportation and strengthen the cleaning and disinfection of the environment, objects and the like, but the disinfection of food, especially the disinfection of food in cold chain environment, particularly the disinfection of meat, seafood and fish food has certain difficulty, the food is not disinfected, and the food is easy to become a new infection source once flowing into society.
Therefore, the market urgently needs to develop a product which can effectively kill or prevent the coronavirus on the food, the product belongs to a disinfection or external preparation, can be used for manufacturing, storing, transporting, selling and using the food, can also be used for carrying out comprehensive disinfection and sterilization and personal daily protection measures of the surrounding environment of the high-risk area with important epidemic situation, and can play a role in preventing the spread and infection of the new coronavirus, so the achievement of the invention has great significance in timely popularization and application.
The virus is sensitive to heat, and can be inactivated at 56 deg.C for 90min or 75 deg.C for 30 min; the effective time of ultraviolet irradiation is 30 min. Common disinfectants, such as 75% by volume ethanol, iodophors, mid-range quaternary ammonium salts, chlorine-containing species, peroxides and other chemical disinfectants, can be effectively killed within a specified time. In addition, hydrogen peroxide aerosol spraying, peracetic acid heating fumigation, pulse ultraviolet sterilization technology, visible light sterilization technology and the like are used as novel disinfectants and sterilization technologies, the inactivation effect on novel coronavirus is good, and the device is suitable for surface sterilization of objects. Although peracetic acid has a good disinfecting effect, it is somewhat corrosive to the surface of the item to be disinfected. It has certain irritation to skin and great harm to human body, and is common chlorine disinfectant in the market. Bleaching powder, sodium hypochlorite, chlorine dioxide, sodium dichloroisocyanurate, sodium trichloroisocyanurate and other disinfectants can generate pungent chlorine in the disinfection process. And residues of the similar disinfectants in the various markets cause certain pollution and damage to the environment and articles.
The natural composition comprises hawthorn kernel dry distillation oil, dry distillation liquid and other components, and comprises raw materials or intermediate products of a Honghua medicinal preparation produced by Shandong Tan Shenzhou pharmaceutical Co., Ltd, wherein the Honghua medicinal preparation is a Fujie lotion, and the national standard character number of the medicine is Z10980131. In vitro cell screening tests show that the traditional Chinese medicine lotion of the natural composition has a remarkable killing effect on the novel coronavirus.
The applicant expects that the natural composition of the present invention can be widely used in disinfectant formulations for external use for environmental disinfection and personal disinfection, and that the natural composition of the present invention is used for disinfection of food in the entire industrial chain, thereby being advantageous for protecting food practitioners and consumers. However, since the traditional Chinese medicine lotion and the food preparation of the natural composition are different, and the traditional Chinese medicine lotion and the food preparation of the natural composition have different auxiliary materials, different preparations and different application environments, whether the natural composition can be used for disinfection of the industrial chain of food needs to be deeply researched, the research is not simply pushed forward, and the applicant carries out deep research and development on the natural composition in order to meet the long-term requirement of epidemic prevention and control.
Disclosure of Invention
The invention provides the application of a natural composition as a disinfectant in blocking the transmission of viruses through the surface of a package, and the natural composition has the advantages of good effect of killing various viruses, stable production quality and no pollution to the surrounding environment. The natural composition can be used for sterilizing food in the processes of processing, production, circulation and use, can also be used for sterilizing the surrounding environment of public health places such as hospitals, aviation, public health and the like and sterilizing the hands, the faces and the like of human bodies, can be popularized and applied in time, and has wide market application prospect.
The invention provides an application of a natural composition as a disinfectant in blocking virus from spreading through a packaging surface, wherein the natural composition comprises the following components in parts by weight: 3-7 parts of hawthorn seed dry distillation oil.
As a preferred aspect of the present invention, the natural composition further comprises the following components: 18-22 parts of hawthorn seed dry distillation liquid.
As a further preferred of the present invention, the natural composition is: 3-7 parts of hawthorn seed dry distillation oil and 18-22 parts of hawthorn seed dry distillation liquid.
The present invention provides the use of a natural composition to block the transmission of viruses through packaging surfaces, including but not limited to the cold chain.
The present invention provides the use of a natural composition to block the transmission of viruses through packaging surfaces, including but not limited to the manufacture, storage, transportation, sale, use of food products.
The invention provides an application of a natural composition in preventing and killing coronavirus on food.
The natural composition comprises the following components in parts by weight: 5 parts of hawthorn seed dry distillation oil, 20 parts of hawthorn seed dry distillation liquid, 50 parts of deionized water and a proper amount of auxiliary materials; the preparation form is external preparation, cleaning solution, gel, hand cleanser, and spray.
The auxiliary materials of the invention are preferably food auxiliary materials, medicine auxiliary materials and cosmetic auxiliary materials.
The preparation method of the natural composition comprises the following steps: mixing the semen crataegi dry distillation oil and semen crataegi dry distillation liquid, adding food grade adjuvants, stirring at room temperature for 20-40 min to obtain mixed solution, standing, and filtering to obtain natural composition;
the natural composition can be in the form of solution, gel, emulsion, spray, aerosol, disinfectant, liniment, or liquid smoke.
As a further preference, the preparation method of the natural composition is:
the method comprises the steps of putting hawthorn kernel dry distillation oil into a batching cylinder, adding 120-mesh screen food auxiliary materials, and stirring for 30 minutes at normal temperature;
adding the hawthorn kernel dry distillation liquid into the liquid medicine in the step, and stirring for 30 minutes at normal temperature to obtain a mixed liquid A;
adding purified water into the mixed solution obtained in the second step, and stirring at normal temperature for 30 minutes to obtain a mixed solution B;
fourthly, standing the mixed liquid B obtained in the step three for 4 hours, and filtering to obtain the liquid B.
The invention provides the use of a natural composition for blocking the transmission of viruses through packaging surfaces, and the use of said natural composition for the preparation of a medicament for the prevention and eradication of coronaviruses.
The virus killed by the natural composition comprises: single-stranded RNA viruses, double-stranded RNA viruses, single-stranded DNA viruses, and double-stranded DNA viruses;
the virus killed by the invention comprises: adenovirus, herpesvirus, poxvirus, reovirus, small DNA virus, togavirus, orthomyxovirus, paramyxovirus, cannonball virus, hepatitis a virus, hepatitis b virus, hepatitis c virus, hepatitis e virus, poliovirus, aids virus, encephalitis b virus, dengue fever virus, yellow fever virus, west nile virus, japanese encephalitis virus, tick-borne encephalitis virus, hemorrhagic fever virus, bovine viral diarrhea virus, zaka virus, measles virus, rabies virus, influenza virus, avian influenza virus, swine influenza virus, ebola virus, rotavirus, coronavirus, SARS virus, MERS virus;
the coronavirus of the present invention is preferably SARS-COV-2 novel coronavirus.
The in vitro cell experiment of the present invention is represented by the novel coronavirus (SARS-COV-2), and the diseases prevented and killed by the present invention are not limited to the novel coronavirus, and can also refer to the coronavirus similar to the novel coronavirus.
The active component of the natural composition is a hawthorn seed extract, and all active component substances of the natural composition are hawthorn seed dry distillation liquid and hawthorn seed dry distillation oil. The extraction process method of the effective substance components comprises the following steps: taking hawthorn kernels, placing the hawthorn kernels in a dry distillation kettle, wherein the feeding amount is 55% of the volume ratio of equipment, adopting a dynamic dry distillation process with nitrogen as a driving force, heating the hawthorn kernels at a heating rate of 40 ℃/h, controlling the reaction process of dry distillation liquid, keeping the pressure in the dry distillation kettle at 0.04-0.12MPa, heating the dry distillation kettle at a heating temperature of 160-190 ℃ for 1 hour, collecting dry distillation products at a temperature of more than 160 ℃, keeping the temperature at 250-280 ℃ for 3 hours, and collecting fractions to obtain the hawthorn kernel dry distillation liquid and the dry distillation oil. The component research of the substances shows that the substances mainly contain various effective components such as organic acid, ursolic acid, furfuraldehyde, phenols and the like.
The natural composition can be prepared into a dosage form of solution, cleaning solution, gel, spray, aerosol, disinfectant, hand sanitizer, liniment, liquid smoke and the like by utilizing the hawthorn seed extract and adding the commonly used auxiliary materials.
Cleaning liquid agent: the hawthorn seed extract is mixed with a proper amount of auxiliary materials to prepare cleaning liquid. The selected solvents were: water, glycerol, ethanol, propylene glycol, fatty oil, etc. The solubilizer is as follows: sodium stearate, lauric acid, triethanolamine organic soap, alkali metal soap, sulfated castor oil, sodium dodecyl sulfate, lecithin and soybean lecithin. Cosolvent: benzoic acid, potassium iodide, and the like. Latent solvent: glycerol, ethanol, propylene glycol, and the like. The preservatives selected were: sodium benzoate, sorbic acid, benzalkonium bromide, parabens, and the like.
Gelling agent: mixing semen crataegi extract with adjuvant, and making into gel. The selected aqueous gel matrix comprises: tragacanth, gelatin, polyethylene glycol, povidone, polyvinyl acetate, cellulose derivatives, propylene glycol, sodium alginate, carbomer, and the like. Oily gel bases, such as: liquid paraffin, polyoxyethylene, fatty oil, colloidal silica, aluminum soap or zinc soap, etc. Preservatives, such as: methyl paraben, benzalkonium bromide, and the like. Humectants, such as: polyethylene glycol, glycerol, propylene glycol, sorbitol, sodium pyrrolidone carboxylate, PDS-3 high-efficiency humectant, etc. Oils, such as: ethoxylated esters, almond oil, and the like. pH regulators, such as triethanolamine, sodium hydroxide, etc. Solubilizers, such as: PEG-40 hydrogenated castor oil or Tween 80.
Liquid soap: the hawthorn seed extract is mixed with a proper amount of pharmaceutical excipients to prepare a liquid soap or a liniment. The material is selected from dispersion medium, high concentration ethanol. Cationic bactericide: quaternary ammonium salts such as: benzalkonium chloride, benzylalkyl ammonium chloride, cetylpyridinium chloride, dialkyl dimethyl ammonium halides, monoalkyl benzylalkyl ammonium halides; biguanides such as chlorhexidine gluconate, chlorhexidine acetate, chlorhexidine hydrochloride, polyhexamethylene guanidine, and the like. Thickening agent: including alkanolamides such as coconut diethanolamide, coconut monoethanolamide, coconut monoisopropanolamide, cocoamide, lauroyl-linoleoyl diethanolamide, lauroyl-myristoyl diethanolamide, isostearyl diethanolamide, linoleoyl diethanolamide, and the like. The surface active agent is: tea saponin, quillaja acid, cholesterol, phospholipid, acacia, tragacanth, alginic acid, chitosan and the like. Nonionic surfactant: alkyl glycoside, coconut oil diethanolamide, lauramidopropyl amine oxide, sucrose ester, alkylphenol polyoxyethylene ether, etc. Zwitterionic surfactant: cocamidopropyl betaine or dodecyl dimethyl betaine. Anionic surfactant: sodium fatty alcohol-polyoxyethylene ether sulfate, secondary alkyl sodium sulfonate, fatty acid methyl ester sodium sulfonate, a-alkenyl sulfonate, sodium dodecyl sulfate, sodium lauroyl sarcosinate, potassium alkyl phosphate, sodium alkyl phosphate, ammonium alkyl sulfate and the like. pH regulator: and (4) citric acid. Humectant: glycerol, ethylene glycol, sorbitol, and the like. Essence: osmanthus essence, apple essence, lavender essence and the like.
Spraying agent: mixing semen crataegi extract with appropriate amount of medicinal adjuvants, and making into spray. The selected medicinal auxiliary material solubilizers are as follows: absolute ethyl alcohol, distilled water, and the like. Humectant: propylene glycol, glycerol, polyethylene glycol, and the like. Surfactant (b): such as polyethylene glycol, tween 80, sodium dodecyl sulfate, and the like. Preservative: methyl paraben, ethyl paraben, benzalkonium chloride, benzalkonium bromide, chlorhexidine acetate, and the like. pH regulator: one or more of disodium citrate, trisodium citrate, sodium citrate, citric acid monohydrate, potassium citrate, sodium carbonate, sodium bicarbonate, potassium bicarbonate, sodium hydroxide, potassium hydroxide, phosphate, dihydrogen phosphate, hydrogen phosphate, tartaric acid, tartrate, hydrogen tartrate, amino acid and salts thereof. Antioxidant: sulfurous acid, sulfites, bisulfites, pyrosulfites, thiosulfates, ascorbic acid, and the like. Aromatic agent: natural or synthetic essence and flavor including rose essence, clove oil, eucalyptus oil, etc.
The natural composition has the following beneficial effects: the external pharmacodynamic test proves that the Chinese medicinal preparation of the natural composition has stronger killing effect on the novel coronavirus (SARS-COV-2), and the selected active components are various effective active ingredients such as phenols, aldehydes, ketones, organic acids and the like contained in the hawthorn seed extract, so the Chinese medicinal preparation has stronger external sterilization, killing and virus replication inhibiting effects.
The hawthorn seed extract of the natural composition can be prepared into the following components: solution, cleaning liquid, lotion, gel, spray, aerosol, disinfectant, hand sanitizer, liniment, and liquid smoke.
The use of one of the natural compositions of the present invention as a prophylactic and/or a disinfectant against new coronaviruses provides for the use of blocking the transmission of coronaviruses through pathways including, but not limited to, the cold chain.
The use of one of the natural compositions of the present invention as a prophylactic and/or disinfectant for the treatment of new coronaviruses provides for the use of blocking the transmission of coronaviruses through pathways including, but not limited to, the surface of a food or article.
The use of a natural composition of the present invention as a prophylactic and/or disinfectant for the treatment of new coronaviruses provides for the use of blocking the transmission of coronaviruses through routes including, but not limited to, food manufacture, storage, transportation, sale, use.
The natural composition also has the advantages of stable quality, lower production cost, obvious industrialization and very wide market application prospect and value.
The application method of the natural composition can be divided into mixing and adding. Mixing: the preparation of the natural composition of the present invention is uniformly added or sprayed onto the surface of the food container. Adding: the preparation of the natural composition of the invention is applied to the surface of the container surface by adopting the modes of smearing, rubbing, smearing, soaking, spraying, atomizing, gasifying and the like.
One of the applications is as follows: the antiseptic solution of the natural composition of the present invention is applied to the surface of the food container and dried for placement of the food.
The food container that can be used for killing is: paper, wood, bamboo, degradable plastic, metal and other packaging containers;
the food materials which can be used for killing are: pork, beef, mutton, chicken, duck, goose, fish, mollusk, animal bone, animal cartilage, dairy, sausage, hot dog, smoked sausage, roasted sausage, casing, etc.
The second application is as follows: the natural composition disinfection solution of the invention is applied to the surface of the sanitary article and is used for disinfecting hands, faces and other parts.
The solution can be sprayed on the surface of sanitary articles, such as napkin, napkin cloth, dish cloth, toilet paper, packaging bag, packaging box, packing box, diaper, sanitary towel, disposable clothes and trousers, disposable bed sheet, tablecloth, chair cloth, and toilet seat pad, and the sanitary articles can be used after drying.
The solution is sprayed onto the surface of sanitary product such as napkin and toilet paper, and the sanitary product is used in wet state.
The application of the product of the invention relates to seafood, fresh seafood, aquatic products, slaughter products, meat products, bone products, viscera products, skin products, hoof products, ear products and the like.
The applications of the products of the invention relate to the entirety and to the finished products of these products. The food is in the form of block, powder, granule, paste, liquid, etc.
The product of the invention is applied to manual operation and mechanical operation, and has high automation degree.
The present invention is further illustrated by the following exemplary embodiments in order that the practice of the invention may be more fully understood. The beneficial effects of the natural composition of the present invention are demonstrated below and by in vitro antiviral tests.
Examples of the experiments
1. Experimental report for in vitro screening of novel coronavirus (SARS-COV-2)
1.1, purpose of experiment
The anti-novel coronavirus (SARS-COV-2) effect of the Chinese medicinal preparation of the natural composition of the present invention was evaluated at the cellular level during the emergency of epidemic situation.
1.2, experimental materials:
1.2.1 test drugs: tests are carried out by adopting different drug concentrations of the traditional Chinese medicine preparation of the natural composition, and the name and the concentration of the drug are shown in table 1.
1.2.2 test cells: VeroE6 cells, maintained by the national focus laboratory Virus laboratory of the Guangzhou respiratory health institute respiratory disease.
1.2.3 sources of viral columns tested: SARS-CoV-2, titer TCID50＝10-6100 μ L, stored at 80 ℃ in BSL-3 laboratory (respiratory disease national center laboratory high pathogenic microorganism research laboratory). Using a Virus titre of 100TCID50。
1.3, an experimental method:
1.3.1 sample preparation
The Chinese medicinal preparation (Honghe Fujie lotion) of the natural composition is respectively diluted by 800 times, 1000 times, 1600 times and 3200 times with purified water and is taken as a determination sample solution of a sample.
1.3.2 test drugs:
table 1: drug name, experimental concentration and grouping
1.3.3 antiviral test of test drugs
(1) Sterile 96-well culture plate, 100. mu.L of 2X 10 concentration is added into each well5cells/mL VeroE6 cells, 5% CO at 37 ℃2Culturing for 24 hours;
(2) addition of 100TCID to the plates, and to the virus control50Virus liquid 100 mul/well, 37 deg.C, 5% CO2Adsorbing for 2 hours by an incubator;
(3) after 2 hours, discarding the cell culture solution in the 96-well culture plate; diluting the tested medicine into each concentration in table 1, wherein each concentration is 3 multiple holes, and adding the liquid medicine into each hole with the concentration of 100 mu l;
(4) simultaneously setting up a cell control, a blank control (solvent control) and a virus control (negative control);
(5) cell 37 ℃, 5% C02Incubating in an incubator for 3-4 days;
(6) optical displayCytopathic effect (CPE) was observed under the microscope and the degree of cytopathic effect was recorded according to the following 6-point scale: "-" no lesions present; "±" means less than 10% cytopathic effect; "+" indicates about 25% cellular pathology; "+ +" indicates about 50% of cellular pathology; "+ + + +" indicates that about 75% of the cells are diseased: "+ ++" indicates that 75% or more of the lesions are diseased. The half maximal effective concentration (IC) was calculated using the Reed-Muench method or GraphPad prism5.050). Judging the drug effect standard: concentrations that inhibit viral CPE by 50% are considered effective concentrations.
1.3.4 Experimental conditions: the above experimental operations are all completed in a BSL-3 laboratory.
1.4, results of the experiment
TABLE 1 results of the pharmacological effects of Red nucleus (batch No. 180802) against the novel coronavirus
The experiment result shows that the Chinese medicinal preparation red nucleus gynecological cleaning lotion (batch No. 180802) of the natural composition has an IC50 value of 743.2 times of dilution for the cytopathic effect of a novel coronavirus (SARS-COV-2) infected Vero E6 cell.
The illustrative embodiments of the present invention and the description thereof are provided to illustrate the present invention and should not be construed as unduly limiting the present invention wherein the medicament is red nucleus feminine wash solution.
Detailed Description
The following are specific examples of the present disclosure, which are used to illustrate technical solutions to be solved in the present disclosure and help those skilled in the art understand the present disclosure, but the present disclosure is not limited to these examples.
EXAMPLE 1 preparation and use of solution A of the Natural composition of the invention
Weighing 7g of hawthorn seed dry distillation oil and 15g of hawthorn seed dry distillation liquid, adding purified water to 1000ml, stirring uniformly, standing, and filtering to obtain the product.
One of the applications is as follows: the solution is sprayed onto the surface of a food container and dried for placement of the food.
The food container for killing comprises: packaging containers made of paper, wood, bamboo, degradable plastics, metal and the like;
the food for killing comprises the following components: pork, beef, mutton, chicken, duck, goose, fish, mollusk, animal bone, animal cartilage, dairy, sausage, hot dog, smoked sausage, roasted sausage, casing, etc.
The second application is as follows: the solution can be applied on the surface of sanitary product for disinfecting hands and face.
The solution can be sprayed on the surface of sanitary articles, such as napkin, napkin cloth, dish cloth, toilet paper, packaging bag, packaging box, packing box, diaper, sanitary towel, disposable clothes and trousers, disposable bed sheet, tablecloth, chair cloth, and toilet seat pad, and the sanitary articles can be used after drying.
The solution is sprayed onto the surface of sanitary product such as napkin, napkin cloth, and dish cloth, and the sanitary product is used in wet state.
EXAMPLE 2 preparation and use of solution B of the Natural composition of the invention
Weighing 7g of hawthorn seed dry distillation oil, 15g of hawthorn seed dry distillation liquid and a proper amount of glycerol, adding purified water to 1000ml, stirring and uniformly mixing, standing, and filtering to obtain the hawthorn seed dry distillation oil.
The same applies as in example 1.
EXAMPLE 3 preparation and use of solution C of the Natural composition of the invention
Weighing 7g of hawthorn seed dry distillation oil, 15g of hawthorn seed dry distillation liquid and a proper amount of carbomer, adding purified water to 1000ml, stirring and uniformly mixing, standing, and filtering to obtain the product.
The same applies as in example 1.
EXAMPLE 4 preparation and application of cleaning solution of Natural composition of the invention
Putting 5g of hawthorn kernel dry distillation oil into a batching cylinder, adding a 120-mesh screen, 25g of tween (80) and 200g of purified water, and stirring for 30 minutes at normal temperature;
20g of hawthorn seed dry distillation liquid is added into the liquid medicine, and the mixture is stirred for 30 minutes at normal temperature;
adding 50g of purified water into the liquid medicine, and stirring for 30 minutes at normal temperature;
fourthly, the mixed liquid is left to stand for 4 hours and then filtered to prepare a cleaning liquid.
The same applies as in example 1.
EXAMPLE 5 preparation and use of spray A of Natural composition of the invention
Weighing 7g of hawthorn kernel dry distillation oil, 15g of hawthorn kernel dry distillation liquid and 96g of carboxymethyl cellulose for later use;
and taking a proper amount of purified water, adding 96g of carboxymethyl cellulose, soaking for 12 hours, and waiting until the carboxymethyl cellulose is completely swelled for later use.
And thirdly, sequentially adding 7g of hawthorn kernel dry distillation oil and 15g of hawthorn kernel dry distillation liquid into the solution obtained in the step 2, stirring while adding, and stirring uniformly.
And fourthly, supplementing purified water to 8000ml, stirring and uniformly mixing, standing and filtering to obtain the water-based paint.
The same applies as in example 1.
EXAMPLE 8 preparation and use of spray B of Natural composition of the invention
Weighing 7g of hawthorn kernel dry distillation oil, 15g of hawthorn kernel dry distillation liquid, 80g of sodium dodecyl sulfate, 160g of glycerol, 8g of sodium benzoate, 8ml of peppermint oil and 96g of carboxymethyl cellulose for later use;
and taking a proper amount of purified water, adding 96g of carboxymethyl cellulose, soaking for 12 hours, and waiting until the carboxymethyl cellulose is completely swelled for later use.
And thirdly, sequentially adding the hawthorn seed extract, the peppermint oil, the sodium dodecyl sulfate, the glycerol and the sodium benzoate into the solution obtained in the step 2, stirring while adding, and uniformly stirring.
And fourthly, supplementing purified water to 8000ml, stirring and uniformly mixing, standing and filtering to obtain the water-based paint.
The same applies as in example 1.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.
Claims (9)
1. Use of a natural composition as a disinfectant in blocking the transmission of viruses through packaging surfaces, characterized in that: the natural composition comprises the following components in parts by weight: 3-7 parts of hawthorn seed dry distillation oil.
2. Use according to claim 1, characterized in that: the natural composition also comprises the following components: 18-22 parts of hawthorn seed dry distillation liquid.
3. Use according to claim 1 or 2, characterized in that: the propagation pathway includes, but is not limited to, the cold chain.
4. Use according to claim 3, characterized in that: the transmission routes include, but are not limited to, the manufacture, storage, transportation, sale, use of food.
5. Use according to claim 1 or 2, characterized in that: use of said natural composition for the prevention and disinfection of coronavirus on a packaging surface.
6. Use according to claim 2, characterized in that: the natural composition comprises the following components in parts by weight: 5 parts of hawthorn seed dry distillation oil, 20 parts of hawthorn seed dry distillation liquid, 50 parts of deionized water and a proper amount of auxiliary materials in balance.
7. The use according to claim 6, wherein the natural composition is prepared by a process comprising:
mixing semen crataegi dry distillation oil and semen crataegi dry distillation liquid, adding medicinal adjuvants, stirring at room temperature for 20-40 min to obtain mixed solution, standing, and filtering to obtain natural composition;
the natural composition can be in the form of solution, gel, spray, aerosol, disinfectant, or liniment.
8. Use according to claim 1 or 2, characterized in that: the virus comprises: adenovirus, herpesvirus, poxvirus, reovirus, small DNA virus, togavirus, orthomyxovirus, paramyxovirus, cannonball virus, hepatitis a virus, hepatitis b virus, hepatitis c virus, hepatitis e virus, poliovirus, aids virus, encephalitis b virus, dengue fever virus, yellow fever virus, west nile virus, japanese encephalitis virus, tick-borne encephalitis virus, hemorrhagic fever virus, bovine viral diarrhea virus, zaka virus, measles virus, rabies virus, influenza virus, avian influenza virus, swine influenza virus, ebola virus, rotavirus, coronavirus, SARS virus, MERS virus;
the coronavirus is preferably SARS-COV-2 novel coronavirus.
9. Use according to claim 4, characterized in that: the food container is as follows: paper, wood, bamboo, degradable plastic, metal and other packaging containers;
the sanitary product comprises: napkin paper, napkin cloth, dish-washing cloth, toilet paper, packing bag, packing box, diaper, sanitary towel, disposable clothes and trousers, disposable bed sheet, tablecloth, chair cloth and toilet seat pad.
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| CN202011619948.7A CN114680141A (en) | 2020-12-30 | 2020-12-30 | Use of a natural composition as a disinfectant in blocking the transmission of viruses through packaging surfaces |
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| CN202011619948.7A CN114680141A (en) | 2020-12-30 | 2020-12-30 | Use of a natural composition as a disinfectant in blocking the transmission of viruses through packaging surfaces |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1502244A (en) * | 2002-11-25 | 2004-06-09 | 戈 王 | Disinfectant and preparation process thereof |
| CN1555847A (en) * | 2004-01-05 | 2004-12-22 | 王贵林 | Medicne for preventing and treating virus and its preparaton method |
| CN1969982A (en) * | 2006-12-08 | 2007-05-30 | 李剑一 | Chinese hawthorn seed and application thereof in preparation of medicament for treating respiratory tract infection and air disinfectant |
| CN1985907A (en) * | 2005-12-22 | 2007-06-27 | 北京佗林医药科技有限公司 | Antiviral effect and antiviral medicine application of haw stone extract |
| CN104107237A (en) * | 2014-07-22 | 2014-10-22 | 山东步长神州制药有限公司 | Application of traditional Chinese medicine preparation in preparation of medicines for treating dermatophytosis |
| CN106860647A (en) * | 2017-04-18 | 2017-06-20 | 席亚彬 | A kind of preparation method and applications of natural source bouvardin |
-
2020
- 2020-12-30 CN CN202011619948.7A patent/CN114680141A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1502244A (en) * | 2002-11-25 | 2004-06-09 | 戈 王 | Disinfectant and preparation process thereof |
| CN1555847A (en) * | 2004-01-05 | 2004-12-22 | 王贵林 | Medicne for preventing and treating virus and its preparaton method |
| CN1985907A (en) * | 2005-12-22 | 2007-06-27 | 北京佗林医药科技有限公司 | Antiviral effect and antiviral medicine application of haw stone extract |
| CN1969982A (en) * | 2006-12-08 | 2007-05-30 | 李剑一 | Chinese hawthorn seed and application thereof in preparation of medicament for treating respiratory tract infection and air disinfectant |
| CN104107237A (en) * | 2014-07-22 | 2014-10-22 | 山东步长神州制药有限公司 | Application of traditional Chinese medicine preparation in preparation of medicines for treating dermatophytosis |
| CN106860647A (en) * | 2017-04-18 | 2017-06-20 | 席亚彬 | A kind of preparation method and applications of natural source bouvardin |
Non-Patent Citations (4)
| Title |
|---|
| 叶磊: "山楂核干馏油抑菌及杀菌作用的研究", 北京联合大学学报, no. 03, pages 48 - 51 * |
| 嫩米香薰: "来自民间的一份请愿", pages 1 - 6, Retrieved from the Internet <URL:https://zhuanlan.zhihu.com/p/105469544> * |
| 张文福;: "更安全更友好的消毒剂", 中国消毒学杂志, no. 05, pages 397 - 401 * |
| 温娜;刘妍如;陆世海;唐志书;刘红波;宋忠兴;王明耿;钱大玮;: "山楂核干馏油GC-MS主要成分分析及含量测定", 天然产物研究与开发, vol. 30, no. 06, pages 990 - 996 * |
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