CN114668884A - Scar dressing containing traditional Chinese medicine exosomes and preparation method thereof - Google Patents
Scar dressing containing traditional Chinese medicine exosomes and preparation method thereof Download PDFInfo
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- CN114668884A CN114668884A CN202210316292.4A CN202210316292A CN114668884A CN 114668884 A CN114668884 A CN 114668884A CN 202210316292 A CN202210316292 A CN 202210316292A CN 114668884 A CN114668884 A CN 114668884A
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- chinese medicine
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- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The invention discloses a scar dressing containing traditional Chinese medicine exosomes and a preparation method thereof, wherein the scar dressing comprises the following components in parts by weight: 3-12 parts of traditional Chinese medicine exosome, 2-6 parts of matrine, 4-7 parts of curcumin, 5-12 parts of tanshinone IIA, 10-30 parts of simethicone, 1-5 parts of glycerol, 2-6 parts of carbomer, 801-5 parts of polysorbate, 2-5 parts of triethanolamine, 0.1-0.5 part of ethylparaben and 5-33 parts of purified water. The invention utilizes exosome from traditional Chinese medicine sources as a carrier of matrine, curcumin and tanshinone IIA, has unique advantages, solves the problems of low solubility, instability, low bioavailability, low targeting property and the like of active ingredients in the prior art, has definite effect on preventing scar formation and can greatly enhance the effect of preventing and improving pathological scars of skin.
Description
Technical Field
The invention relates to the field of biological medicines, in particular to a scar dressing containing traditional Chinese medicine exosomes and a preparation method thereof.
Background
The skin is the first line of defense of human bodies against pathogen invasion, however, in daily life, the skin is often affected by various external factors to cause skin trauma, and more or less physical and psychological burdens are brought to individuals. Scars are a general term of appearance morphology and histopathological changes of normal skin tissues caused by various wounds, and are inevitable products of the fact that the tissues cannot be completely and automatically repaired normally in the wound repair process and are replaced by fibrous tissues for repair. Excessive scar growth due to various causes during the wound healing process, known as pathological scars, includes hypertrophic scars and keloids. Pathological scars are different in shape and red or purple-red in surface, so that the beauty is influenced, pain, pruritus and even neuropathic pain are accompanied, contracture can be formed in severe cases to generate dysfunction, and the normal life quality of a patient is seriously influenced, so that the scar treatment product has an application prospect.
Exosomes are vesicles with a particle size of 30-200nm derived from an inner membrane, are composed of lipid bilayers and proteins, Deoxyribonucleotides (DNA) and Ribonucleotides (RNA) contained in the lipid bilayers, and have the function of communicating cells. The exosome extracted from the traditional Chinese medicine is a traditional Chinese medicine exosome, the traditional Chinese medicine exosome has a stable bimolecular phospholipid structure, an internal cavity is beneficial to loading a large amount of water-soluble substances, a hydrophobic region in the middle of bimolecular phospholipid can load hydrophobic substances, and the special structure can protect the loaded medicine from being degraded or diluted after being stored for a long time in a severe environment, can well permeate through a skin barrier and acts on an affected part in a targeted mode. The traditional Chinese medicine exosome is used as a medicine carrier, and can effectively solve the problems of poor water solubility, low bioavailability, low capability of penetrating and retaining skin, unsatisfactory distribution in vivo, poor targeting positioning on cells and the like of the medicine. Modern pharmacological research shows that some active monomers of the traditional Chinese medicine have obvious effect on scars, and the prepared traditional Chinese medicine exosome also plays an important role in adjusting in the process of wound repair, can promote angiogenesis, optimize the performance of fibroblast, regulate and control the fibrosis process, and has very obvious effect of inhibiting scars.
Disclosure of Invention
In order to solve the defects of the prior art, the invention discloses a scar dressing containing traditional Chinese medicine exosomes and a preparation method thereof, wherein the scar dressing comprises the traditional Chinese medicine exosomes, matrine, curcumin, tanshinone, dimethyl silicone oil and the like. And modern pharmacological research shows that some active monomers of the traditional Chinese medicine have obvious effects on scars, such as matrine, curcumin and tanshinone IIA, and especially, the traditional Chinese medicine exosome is used as a carrier of the matrine, the curcumin and the tanshinone IIA, so that the traditional Chinese medicine exosome has unique advantages.
The matrine is extracted from sophora flavescens of leguminous plants by organic solvents such as ethanol, and researches show that the matrine can inhibit the proliferation of scar fibroblasts, so that the expression of apoptosis-related factors bax in the fibroblasts is up-regulated, and the expression of p53 and bc1-2 is down-regulated, thereby promoting the apoptosis of the fibroblasts.
Curcumin is a diketone compound extracted from rhizome of Curcuma rhizome of Zingiberaceae and Araceae, and has pharmacological effects of resisting inflammation and oxidation, inhibiting proliferation of malignant tumor cells, inhibiting proliferation of keloid fibroblast, and arresting cell cycle at G1 stage.
Tanshinone IIA, a diterpene compound extracted from dried root and rhizome of Salvia miltiorrhiza Bunge of Labiatae, has pharmacological effects of improving coronary artery circulation, resisting oxidation, resisting infection, etc. Research shows that tanshinone IIA can promote wound healing and inhibit fibroblast proliferation, so as to reduce scar formation.
The invention provides a scar dressing containing traditional Chinese medicine exosomes, which solves the problems of poor effect, low solubility of active ingredients, instability, low targeting property and the like of preventing and treating pathological scars in the prior art, and adopts the following technical means:
a scar dressing containing traditional Chinese medicine exosomes comprises the following components in parts by weight: 3-12 parts of traditional Chinese medicine exosome, 2-6 parts of matrine, 4-7 parts of curcumin, 5-12 parts of tanshinone IIA, 10-30 parts of simethicone, 1-5 parts of glycerol, 2-6 parts of carbomer, 801-5 parts of polysorbate, 2-5 parts of triethanolamine, 0.1-0.5 part of ethylparaben and 5-33 parts of purified water.
Preferably, the dressing consists of the following components in parts by weight: 7 parts of traditional Chinese medicine exosome, 4 parts of matrine, 6 parts of curcumin, 9 parts of tanshinone IIA, 20 parts of simethicone, 3 parts of glycerol, 4 parts of carbomer, 803 parts of polysorbate-803, 4 parts of triethanolamine, 0.3 part of ethylparaben and 15 parts of purified water.
Preferably, the source of the Chinese medicinal exosomes is one or more of salvia miltiorrhiza, turmeric, centella asiatica, rheum officinale and aloe.
A preparation method of scar dressing containing Chinese medicinal exosomes comprises the following steps:
(1) adding carbomer and purified water into the water phase reaction kettle, swelling, stirring until no granules exist, adding triethanolamine, and adjusting pH to 6.0-8.0 for later use;
(2) adding the dimethyl silicone oil, the humectant glycerol, the emulsifier polysorbate-80 and the preservative ethylparaben into the oil phase reaction kettle, stirring, adding the water phase, and uniformly mixing to obtain a pre-gel system;
(3) squeezing the Chinese medicinal materials, filtering, centrifuging at 2-9 deg.C and 2000-. The supernatant was centrifuged at 10000-. Centrifuging the supernatant at 2-9 deg.C and 10000-. And (4) resuspending the precipitate with purified water, and taking the lower layer to obtain the Chinese medicinal exosome. Loading matrine, curcumin and tanshinone IIA into the Chinese medicinal exosome by electroporation to obtain an exosome loaded with matrine, curcumin and tanshinone IIA;
(4) adding the traditional Chinese medicine exosome loaded with the matrine, the curcumin and the tanshinone IIA into a pre-gelling system, and uniformly mixing to obtain the scar dressing containing the traditional Chinese medicine exosome.
Drawings
FIG. 1 is a transmission electron microscope morphology of the experimental exosomes of the present invention.
FIG. 2 is a diagram showing the effect of the present invention on scar treatment on rabbit skin.
Detailed Description
The invention is further illustrated by the following examples, which are not intended to limit the scope of the invention. The experimental methods used in the following examples are all conventional methods unless otherwise specified; the materials, reagents and the like used are, unless otherwise specified, commercially available reagents and materials.
Example 1
A preparation method of scar dressing containing Chinese medicinal exosomes comprises the following steps:
(1) adding 2 parts of carbomer and 5 parts of purified water into a water phase reaction kettle, swelling, stirring until no particles exist, adding triethanolamine, and adjusting the pH value to 6.0-8.0 for later use;
(2) taking 10 parts of dimethyl silicone oil, 801 parts of polysorbate-801 parts, 0.1 part of ethylparaben and 1 part of glycerol, adding into an oil phase reaction kettle, stirring, and mixing the water phase and the oil phase to obtain a pre-gel system;
(3) pulverizing one or more of Saviae Miltiorrhizae radix, Curcuma rhizome, herba Centellae, radix et rhizoma Rhei, and Aloe, centrifuging at 2 deg.C and 2000g for 30 min, and collecting supernatant. The supernatant was centrifuged at 10000g at 4 ℃ for 40 minutes to obtain a supernatant. The supernatant was centrifuged at 10000g at 2 ℃ for 40 minutes to obtain a precipitate. And (4) resuspending the precipitate with purified water, and taking the lower layer to obtain the Chinese medicinal exosome. Taking 3 parts of traditional Chinese medicine exosomes, and loading 2 parts of matrine, 4 parts of curcumin and 5 parts of tanshinone IIA into the traditional Chinese medicine exosomes in an electroporation mode to obtain exosomes loaded with matrine, curcumin and tanshinone IIA;
(4) adding the traditional Chinese medicine exosome loaded with the matrine, the curcumin and the tanshinone IIA into a pre-gelling system, and uniformly mixing to obtain the scar dressing containing the traditional Chinese medicine exosome.
Example 2
A preparation method of scar dressing containing Chinese medicine exosomes comprises the following steps:
(1) adding 3 parts of carbomer and 10 parts of purified water into a water phase reaction kettle, swelling, stirring until no particles exist, adding triethanolamine, and adjusting the pH value to 6.0-8.0 for later use;
(2) adding 15 parts of dimethyl silicone oil, 802 parts of polysorbate-802 parts, 0.2 part of ethylparaben and 2 parts of glycerol into an oil phase reaction kettle, stirring, and mixing the water phase and the oil phase to obtain a pre-gel system;
(3) pulverizing one of Saviae Miltiorrhizae radix, radix Sophorae Flavescentis and Curcuma rhizome, centrifuging at 3 deg.C and 3000g for 40 min, and collecting supernatant. The supernatant was centrifuged at 12000g at 2 ℃ for 50 minutes to obtain a supernatant. The supernatant was centrifuged at 12000g at 4 ℃ for 50 minutes to obtain a precipitate. And (4) resuspending the precipitate with purified water, and taking the lower layer to obtain the Chinese medicinal exosome. Taking 5 parts of traditional Chinese medicine exosomes, and loading 3 parts of matrine, 5 parts of curcumin and 7 parts of tanshinone IIA into the exosomes in an electroporation mode to obtain the exosomes loaded with the matrine, the curcumin and the tanshinone IIA;
(4) adding the traditional Chinese medicine exosome loaded with the matrine, the curcumin and the tanshinone IIA into a pre-gelling system, and uniformly mixing to obtain the scar dressing containing the traditional Chinese medicine exosome.
Example 3
A preparation method of scar dressing containing Chinese medicinal exosomes comprises the following steps:
(1) adding 4 parts of carbomer and 15 parts of purified water into a water phase reaction kettle, swelling, stirring until no particles exist, adding triethanolamine, and adjusting the pH value to 6.0-8.0 for later use;
(2) adding 20 parts of dimethyl silicone oil, 803 parts of polysorbate, 0.3 part of ethylparaben and 3 parts of glycerol into an oil phase reaction kettle, stirring, and mixing the water phase and the oil phase to obtain a pre-gel system;
(3) pulverizing one or more of Saviae Miltiorrhizae radix, Curcuma rhizome, herba Centellae, radix et rhizoma Rhei, and Aloe, centrifuging at 5 deg.C and 4000g for 50 min, and collecting supernatant. The supernatant was centrifuged at 13000g at 5 ℃ for 60 minutes to obtain a supernatant. The supernatant was centrifuged at 14000g at 6 ℃ for 60 minutes to obtain a precipitate. And (4) resuspending the precipitate with purified water, and taking the lower layer to obtain the Chinese medicinal exosome. Taking 7 parts of traditional Chinese medicine exosomes, and loading 4 parts of matrine, 6 parts of curcumin and 9 parts of tanshinone IIA into exosomes by electroporation to obtain exosomes loaded with matrine, curcumin and tanshinone IIA;
(4) adding the traditional Chinese medicine exosome loaded with the matrine, the curcumin and the tanshinone IIA into a pre-gelling system, and uniformly mixing to obtain the scar dressing containing the traditional Chinese medicine exosome.
Example 4
A preparation method of scar dressing containing Chinese medicinal exosomes comprises the following steps:
(1) adding 5 parts of carbomer and 20 parts of purified water into a water phase reaction kettle, swelling, stirring until no particles exist, adding triethanolamine, and adjusting the pH value to 6.0-8.0 for later use;
(2) adding 25 parts of dimethyl silicone oil, 804 parts of polysorbate, 0.4 part of ethylparaben and 4 parts of glycerol into an oil phase reaction kettle, stirring, and mixing the water phase and the oil phase to obtain a pre-gel system;
(3) pulverizing one or more of Saviae Miltiorrhizae radix, Curcuma rhizome, herba Centellae, radix et rhizoma Rhei, and Aloe, centrifuging at 7 deg.C and 5000g for 60 min, and collecting supernatant. The supernatant was centrifuged at 15000 ℃ for 70 minutes at 7 ℃ to obtain a supernatant. The supernatant was centrifuged at 16000g at 6 ℃ for 70 minutes to obtain a precipitate. And (4) resuspending the precipitate with purified water, and taking the lower layer to obtain the Chinese medicinal exosome. Taking 8 parts of traditional Chinese medicine exosomes, and loading 5 parts of matrine, 7 parts of curcumin and 11 parts of tanshinone IIA into exosomes by an electroporation mode to obtain exosomes loaded with matrine, curcumin and tanshinone IIA;
(4) adding the traditional Chinese medicine exosome loaded with the matrine, the curcumin and the tanshinone IIA into a pre-gelling system, and uniformly mixing to obtain the scar dressing containing the traditional Chinese medicine exosome.
Example 5
A preparation method of scar dressing containing Chinese medicinal exosomes comprises the following steps:
(1) adding 6 parts of carbomer and 25 parts of purified water into a water phase reaction kettle, swelling, stirring until no particles exist, adding triethanolamine, and adjusting the pH value to 6.0-8.0 for later use;
(2) adding 30 parts of dimethyl silicone oil, 805 parts of polysorbate, 0.5 part of ethylparaben and 5 parts of glycerol into an oil phase reaction kettle, stirring, and mixing the water phase and the oil phase to obtain a pre-gel system;
(3) pulverizing one or more of Saviae Miltiorrhizae radix, Curcuma rhizome, herba Centellae, radix et rhizoma Rhei, and Aloe, centrifuging at 8 deg.C and 6000g at different speed, and centrifuging for 70 min to obtain supernatant. The supernatant was centrifuged at 14000g at 9 ℃ for 80 minutes to obtain a supernatant. The supernatant was centrifuged at 18000g for 80 minutes at 8 ℃ to obtain a precipitate. And (4) resuspending the precipitate with purified water, and taking the lower layer to obtain the Chinese medicinal exosome. Taking 10 parts of traditional Chinese medicine exosomes, and loading 6 parts of matrine, 6 parts of curcumin and 12 parts of tanshinone IIA into the exosomes in an electroporation mode to obtain the exosomes loaded with the matrine, the curcumin and the tanshinone IIA;
(4) adding the traditional Chinese medicine exosome loaded with the matrine, the curcumin and the tanshinone IIA into a pre-gelling system, and uniformly mixing to obtain the scar dressing containing the traditional Chinese medicine exosome.
Example 6
A preparation method of scar dressing containing Chinese medicinal exosomes comprises the following steps:
(1) taking 6 parts of carbomer and 33 parts of purified water, adding into a water phase reaction kettle, swelling, stirring until no particles exist, adding triethanolamine, and adjusting the pH value to 6.0-8.0 for later use;
(2) adding 30 parts of dimethyl silicone oil, 804 parts of polysorbate, 0.3 part of ethylparaben and 4 parts of glycerol into an oil phase reaction kettle, stirring, and mixing the water phase and the oil phase to obtain a pre-gel system;
(3) pulverizing one or more of Saviae Miltiorrhizae radix, Curcuma rhizome, herba Centellae, radix et rhizoma Rhei, and Aloe, centrifuging at 9 deg.C and 3000g for 70 min, and collecting supernatant. The supernatant was centrifuged at 13000g at 4 ℃ for 90 minutes to obtain a supernatant. The supernatant was centrifuged at 20000g at 9 ℃ for 90 minutes to obtain a precipitate. And (4) resuspending the precipitate with purified water, and taking the lower layer to obtain the Chinese medicinal exosome. Taking 12 parts of traditional Chinese medicine exosomes, and loading 6 parts of matrine, 7 parts of curcumin and 12 parts of tanshinone IIA into exosomes in an electroporation mode to obtain exosomes loaded with matrine, curcumin and tanshinone IIA;
(4) adding the traditional Chinese medicine exosome loaded with the matrine, the curcumin and the tanshinone IIA into a pre-gelling system, and uniformly mixing to obtain the scar dressing containing the traditional Chinese medicine exosome.
Example 7
A preparation method of scar dressing containing Chinese medicinal exosomes comprises the following steps:
(1) adding 4 parts of carbomer and 25 parts of purified water into a water phase reaction kettle, swelling, stirring until no particles exist, adding triethanolamine, and adjusting the pH value to 6.0-8.0 for later use;
(2) adding 20 parts of dimethyl silicone oil, 803 parts of polysorbate, 0.4 part of ethylparaben and 4 parts of glycerol into an oil phase reaction kettle, stirring, and mixing the water phase and the oil phase to obtain a pre-gel system;
(3) pulverizing one or more of Saviae Miltiorrhizae radix, Curcuma rhizome, herba Centellae, radix et rhizoma Rhei, and Aloe, centrifuging at 5 deg.C and 4000g for 60 min, and collecting supernatant. The supernatant was centrifuged at 13000g at 4 ℃ for 70 minutes to obtain a supernatant. The supernatant was centrifuged at 18000g at 7 ℃ for 60 minutes to obtain a precipitate. And (4) resuspending the precipitate with purified water, and taking the lower layer to obtain the Chinese medicinal exosome. Taking 10 parts of traditional Chinese medicine exosomes, and loading 4 parts of matrine, 5 parts of curcumin and 10 parts of tanshinone IIA into exosomes by an electroporation mode to obtain exosomes loaded with matrine, curcumin and tanshinone IIA;
(4) adding the traditional Chinese medicine exosome loaded with the matrine, the curcumin and the tanshinone IIA into a pre-gelling system, and uniformly mixing to obtain the scar dressing containing the traditional Chinese medicine exosome.
Example 8
A preparation method of scar dressing containing Chinese medicinal exosomes comprises the following steps:
(1) adding 2 parts of carbomer and 15 parts of purified water into a water phase reaction kettle, swelling, stirring until no particles exist, adding triethanolamine, and adjusting the pH value to 6.0-8.0 for later use;
(2) adding 10 parts of dimethyl silicone oil, 802 parts of polysorbate, 0.1 part of ethylparaben and 5 parts of glycerol into an oil phase reaction kettle, stirring, and mixing the water phase and the oil phase to obtain a pre-gel system;
(3) pulverizing one or more of Saviae Miltiorrhizae radix, Curcuma rhizome, herba Centellae, radix et rhizoma Rhei, and Aloe, centrifuging at 7 deg.C and 5000g for 50 min, and collecting supernatant. The supernatant was centrifuged at 13000g at 4 ℃ for 60 minutes to obtain a supernatant. The supernatant was centrifuged at 16000g at 4 ℃ for 50 minutes to obtain a precipitate. And (4) resuspending the precipitate with purified water, and taking the lower layer to obtain the Chinese medicinal exosome. Taking 8 parts of traditional Chinese medicine exosomes, and loading 2 parts of matrine, 4 parts of curcumin and 8 parts of tanshinone IIA into exosomes by an electroporation mode to obtain exosomes loaded with matrine, curcumin and tanshinone IIA;
(4) adding the traditional Chinese medicine exosome loaded with the matrine, the curcumin and the tanshinone IIA into a pre-gelling system, and uniformly mixing to obtain the scar dressing containing the traditional Chinese medicine exosome.
To study the performance and efficacy of a scar dressing containing traditional Chinese medicine exosomes prepared in example 3, the following experiments were performed:
first, biological characteristic identification experiment of exosome
Separating one or more fresh medicinal materials of salvia miltiorrhiza, turmeric, centella asiatica, rheum officinale and aloe by adopting a differential centrifugation method to obtain vesicle particles with a certain form, and in order to identify whether the vesicle particles are required exosomes, according to the requirements of extracellular vesicle experiments: (1) performing imaging analysis on the sample by using an electron microscope and the like to confirm the structure of the film bubble; (2) measuring the particle size of the film bubble by a nano-scale analysis technology; (3) the following experiment was performed by detecting protein markers of vesicles using techniques such as immunoblotting and flow-through.
1. Form and particle size of exosome
Taking 10uL of the exosome heavy suspension to drop on a special copper net for an electron microscope, standing for 3 minutes, then counterstaining with 10uL of 2% phosphotungstic acid solution, standing for 5 minutes, and then sucking residual redundant floating liquid with absorbent filter paper. Standing and drying at normal temperature for about 10 minutes, placing under a transmission electron microscope, imaging by using 80-120kV, and observing. Under the field of an electron microscope, the exosome provided by the invention has the advantages of good dispersity, three-dimensional double-membrane structure, complete shape, different sizes, typical spherical or elliptical cup-shaped structure, diameter of 30-150nm, and accordance with the exosome structure mentioned in the existing literature, and the result is shown in figure 1.
Determination of exosome marker protein CD9 by Westernblotting
And quantitatively detecting the exosome protein suspension by adopting a bicinchoninic acid (BCA) method, carrying out water bath at 100 ℃ for 10 minutes, preparing gel and loading the sample. Followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), gel cutting, transfer to PVDF membrane, and blocking for 2 hours. The primary antibody was incubated at 4 ℃ overnight, then washed with Tris-Tween (TBST) buffer, followed by addition of the secondary antibody, incubation at room temperature for 2 hours, washing with TBST buffer, and development by electrochemiluminescence. The result shows that the specificity marker protein CD9 of the exosome extracted by the invention is expressed positively, which indicates that the exosome extracted by the invention meets the requirement in the literature.
Second, skin irritation test
4 healthy adult New Zealand rabbits (2.5-3.0kg) were used for skin stimulation test and divided into test group and control group at random. Selecting 3 experimental parts of each rabbit, each part is about 3 × 3cm2And selecting regions for depilation. The experimental group was coated with a scar dressing containing Chinese medicinal exosomes, and the control group was given physiological saline. The skin condition of the rabbits was observed at 1, 24 and 48h after application, and scored according to the skin adverse reaction grading standard table (table 1). The results show that the adverse reactions of the skin of the experimental group and the control group do not have the phenomena of erythema and edema, the scores are all 0 point, and the skin irritation reaction is not caused.
TABLE 1 adverse skin reaction grading
Experiment of the treatment effect of rabbit skin scar
18 clean scar models of New Zealand rabbits are selected, the male and female parts of the new Zealand rabbits are respectively, and the weight of the new Zealand rabbits ranges from 2.5 kg to 3.0 kg. The scar model group is not specially treated, the experimental group is given scar dressing containing Chinese medicine exosome, and the positive medicine control group is given silicone gel dressing. The medicine is administered 2 times daily, 0.2g each time, and continuously for 30 days. Another 2 new zealand rabbits with clean grade normal skin were used as a blank control group. After the experiment, the rabbits were sacrificed for histological observation.
After 30 days, histological observation shows that the number of fibroblasts in the blank control group (normal skin tissue) is small, and collagen fibers are arranged regularly; the dermis layer in the scar model group is thickened, the number of fibroblasts is large, a large amount of collagen fibers are accumulated and arranged in disorder, and inflammatory cell infiltration is accompanied; the experimental group (scar dressing containing Chinese medicine exosome) has the advantages that the fibroblasts are obviously reduced, the arrangement of collagen fibers is regular, and the treatment effect is better than that of a positive medicine control group (silicone gel dressing). The results are shown in FIG. 2.
Clinical experiment for preventing and treating pathological scars
1. Clinical data
90 scar patients were selected for clinical trials, and included as standard: (1) the clinical diagnosis standard of pathological scars is met; (2) informed consent was signed and this experiment was performed. Exclusion criteria: (1) pregnant patients; (2) scar constitution; (3) past history of scar hyperplasia, etc. Patients were randomized into control (n-45) and experimental (n-45) groups with no significant difference in clinical data between the two groups (P > 0.05).
2. Experimental methods
The experimental group patients were given a scar dressing containing Chinese medicinal exosomes; control patients were given a silicone gel dressing. The two groups of patients adopt an external application mode, and the medicine is externally applied to the affected part without ulcer and damage twice a day, and the treatment course is 3 months.
3. Scar assessment score for two groups of patients
And observing and recording the VSS scores of two groups of patients before and after 3 months of treatment according to the evaluation quantity (VSS) score of the international Vancouver scar, wherein the VSS scores comprise four aspects of scar color, blood vessel distribution, thickness and softness, and are divided into 0-5 points according to the severity degree, and the higher the VSS score is, the more serious the pathological scar of the patient is, and the total score is 15 points.
After 3 months of treatment, the experimental group had a significantly lower VSS score (4.1 ± 1.8) than the pre-treatment VSS score. Compared with a control group, the experimental group has very obvious difference (P is less than 0.01), and the experimental result shows that the treatment effect of the experimental group on the pathological scars is better than that of the control group.
Table 2 two groups of patients VSS score
Denotes P < 0.01.
The results show that the scar dressing containing the traditional Chinese medicine exosomes can effectively inhibit the formation of fibroblasts and inflammatory cells, has obvious effect on preventing and treating pathological scars of the skin, has better treatment effect than a silicone gel dressing, and has clinical application value.
The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments, and all technical solutions belonging to the idea of the present invention belong to the protection scope of the present invention. Several modifications and enhancements may be made without departing from the principles of the invention.
Claims (6)
1. A scar dressing containing Chinese medicine exosomes and a preparation method thereof are characterized in that the dressing is composed of the following components in parts by weight: 3-12 parts of traditional Chinese medicine exosome, 2-6 parts of matrine, 4-7 parts of curcumin, 5-12 parts of tanshinone IIA, 10-30 parts of simethicone, 1-5 parts of glycerol, 2-6 parts of carbomer, 801-5 parts of polysorbate, 2-5 parts of triethanolamine, 0.1-0.5 part of ethylparaben and 5-33 parts of purified water.
2. The scar dressing containing traditional Chinese medicine exosomes according to claim 1, which is characterized by being optimized in parts by weight as follows: 7 parts of traditional Chinese medicine exosome, 4 parts of matrine, 6 parts of curcumin, 9 parts of tanshinone IIA, 20 parts of simethicone, 3 parts of glycerol, 4 parts of carbomer, 803 parts of polysorbate-803, 4 parts of triethanolamine, 0.3 part of ethylparaben and 15 parts of purified water.
3. The scar dressing containing traditional Chinese medicine exosomes according to claim 1, wherein the source of the traditional Chinese medicine exosomes is one or more of salvia miltiorrhiza, turmeric, centella asiatica, rheum officinale and aloe.
4. The traditional Chinese medicine exosome according to claim 3, which is prepared by the following steps: squeezing the fresh medicinal materials, filtering, performing differential centrifugation on the filtrate at 2-9 ℃ and 6000g of filtrate at 2000-. The supernatant was centrifuged at 10000-. Centrifuging the supernatant at 2-9 deg.C and 10000-. And (4) resuspending the precipitate with purified water, and taking the lower layer to obtain the Chinese medicinal exosome.
5. A preparation method of scar dressing containing traditional Chinese medicine exosomes is characterized by comprising the following steps:
(1) adding carbomer and purified water into the water phase reaction kettle, swelling, stirring until no granules exist, and adding triethanolamine to adjust pH to 6.0-8.0 for later use;
(2) adding the dimethyl silicone oil, the humectant glycerol, the emulsifier polysorbate-80 and the preservative ethylparaben into the oil phase reaction kettle, stirring, adding the water phase, and uniformly mixing to obtain a pre-gel system;
(3) loading matrine, curcumin and tanshinone IIA into the traditional Chinese medicine exosome according to claim 4 by electroporation to obtain an exosome loaded with matrine, curcumin and tanshinone IIA;
(4) adding the traditional Chinese medicine exosome loaded with the matrine, the curcumin and the tanshinone IIA into a pre-gelling system, and uniformly mixing to obtain the scar dressing containing the traditional Chinese medicine exosome.
6. A scar dressing containing Chinese medicinal exosome and its preparation method are provided, which is mainly used for auxiliary improvement of skin pathological scar and prevention of skin pathological scar (such as skin scar caused by burn, scald, wound or operation).
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