CN114667351A - 蛋白质的制造方法 - Google Patents

蛋白质的制造方法 Download PDF

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Publication number
CN114667351A
CN114667351A CN202080076873.4A CN202080076873A CN114667351A CN 114667351 A CN114667351 A CN 114667351A CN 202080076873 A CN202080076873 A CN 202080076873A CN 114667351 A CN114667351 A CN 114667351A
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medium
culture
concentration
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phosphoric acid
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Chinese (zh)
Inventor
古关靖道
片山贵裕
大矢裕介
绪方文
平川圣
樋口拓哉
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Ajinomoto Co Inc
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Ajinomoto Co Inc
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/42—Organic phosphate, e.g. beta glycerophosphate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/46—Amines, e.g. putrescine
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
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    • C12N2510/00—Genetically modified cells
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    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

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  • Genetics & Genomics (AREA)
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  • Biochemistry (AREA)
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  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN202080076873.4A 2019-11-05 2020-11-05 蛋白质的制造方法 Pending CN114667351A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019-200753 2019-11-05
JP2019200753 2019-11-05
PCT/JP2020/041377 WO2021090888A1 (ja) 2019-11-05 2020-11-05 タンパク質の製造方法

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CN114667351A true CN114667351A (zh) 2022-06-24

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US (1) US12584156B2 (https=)
EP (1) EP4056678A4 (https=)
JP (1) JP7513034B2 (https=)
KR (1) KR20220097421A (https=)
CN (1) CN114667351A (https=)
WO (1) WO2021090888A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116064706A (zh) * 2022-11-10 2023-05-05 江苏江山聚源生物技术有限公司 通过调节磷酸浓度和添加方式提高重组胶原蛋白发酵水平的工艺

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01222793A (ja) * 1988-02-29 1989-09-06 Morinaga & Co Ltd モノクローナル抗体生産増強法
JPH01247097A (ja) * 1988-03-30 1989-10-02 Morinaga & Co Ltd モノクローナル抗体生産増強法
US6924124B1 (en) * 2002-08-23 2005-08-02 Immunex Corporation Feeding strategies for cell culture
WO2008033517A2 (en) * 2006-09-13 2008-03-20 Abbott Laboratories Cell culture improvements
WO2008136398A1 (ja) * 2007-04-26 2008-11-13 Chugai Seiyaku Kabushiki Kaisha 高濃度アミノ酸含有培地を用いた細胞の培養方法
CN101360820A (zh) * 2006-01-04 2009-02-04 巴克斯特国际公司 无寡肽的细胞培养基
WO2011134921A1 (en) * 2010-04-26 2011-11-03 Novartis Ag Improved cell culture medium
CN102858952A (zh) * 2010-04-26 2013-01-02 诺瓦提斯公司 改进的细胞培养基
CN102994441A (zh) * 2012-09-19 2013-03-27 上海瀚康生物医药科技有限公司 一种细胞培养基及其制备方法和用途

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1434857B1 (en) * 2001-10-02 2007-08-01 Novo Nordisk Health Care AG Method for production of recombinant proteins in eukaryote cells
TWI369401B (en) * 2005-07-05 2012-08-01 Ares Trading Sa Serum-free culture medium for the production of recombinant gonadotropins
RU2016144057A (ru) 2014-04-10 2018-05-15 Байер Хелскеа Ллк Смешанный состав порошка среды и способ получения жидкой среды для культуры клеток
EP3382031B1 (en) 2015-11-25 2023-11-29 Ajinomoto Co., Inc. Method for producing fibroin-like protein
WO2017171001A1 (ja) 2016-03-31 2017-10-05 味の素株式会社 フィブロイン様タンパク質改変体および細胞培養方法

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01222793A (ja) * 1988-02-29 1989-09-06 Morinaga & Co Ltd モノクローナル抗体生産増強法
JPH01247097A (ja) * 1988-03-30 1989-10-02 Morinaga & Co Ltd モノクローナル抗体生産増強法
US6924124B1 (en) * 2002-08-23 2005-08-02 Immunex Corporation Feeding strategies for cell culture
CN101360820A (zh) * 2006-01-04 2009-02-04 巴克斯特国际公司 无寡肽的细胞培养基
WO2008033517A2 (en) * 2006-09-13 2008-03-20 Abbott Laboratories Cell culture improvements
WO2008136398A1 (ja) * 2007-04-26 2008-11-13 Chugai Seiyaku Kabushiki Kaisha 高濃度アミノ酸含有培地を用いた細胞の培養方法
WO2011134921A1 (en) * 2010-04-26 2011-11-03 Novartis Ag Improved cell culture medium
CN102858952A (zh) * 2010-04-26 2013-01-02 诺瓦提斯公司 改进的细胞培养基
CN102858953A (zh) * 2010-04-26 2013-01-02 诺瓦提斯公司 改进的细胞培养基
CN102994441A (zh) * 2012-09-19 2013-03-27 上海瀚康生物医药科技有限公司 一种细胞培养基及其制备方法和用途

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HANDL ALINE: "Development of M3C strategies in production processes with CHO cell cultures to prevent the formation of product aggregation", Retrieved from the Internet <URL:http://oparu.uni-ulm.de/xmlui/bitstream/handle/123456789/19305/Dissertation_Handl.pdf?sequence=8&isAllowed=y> *
S. SATO 等: "Stimulation of monoclonal antibody production by human-human hybridoma cells with an elevated concentration of potassium or sodium phosphate in serum-free medium", CYTOTECHNOLOGY, vol. 2, 31 December 1989 (1989-12-31), pages 63 - 67, XP009536658, DOI: 10.1007/BF00365416 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116064706A (zh) * 2022-11-10 2023-05-05 江苏江山聚源生物技术有限公司 通过调节磷酸浓度和添加方式提高重组胶原蛋白发酵水平的工艺

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US12584156B2 (en) 2026-03-24
JP7513034B2 (ja) 2024-07-09
EP4056678A1 (en) 2022-09-14
JPWO2021090888A1 (https=) 2021-05-14
WO2021090888A1 (ja) 2021-05-14
KR20220097421A (ko) 2022-07-07
US20220251622A1 (en) 2022-08-11
EP4056678A4 (en) 2023-12-06

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