CN114651895A - Method for preparing high-dietary-fiber fermented feed by using pineapple peel residues and application of high-dietary-fiber fermented feed - Google Patents

Method for preparing high-dietary-fiber fermented feed by using pineapple peel residues and application of high-dietary-fiber fermented feed Download PDF

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CN114651895A
CN114651895A CN202210321117.4A CN202210321117A CN114651895A CN 114651895 A CN114651895 A CN 114651895A CN 202210321117 A CN202210321117 A CN 202210321117A CN 114651895 A CN114651895 A CN 114651895A
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pineapple
fermented
pineapple peel
dietary fiber
feed
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韩丽
彭翔
李阳
李爽
张广民
蔡辉益
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Tianjin Bofeide Science & Technology Co ltd
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Tianjin Bofeide Science & Technology Co ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/33Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from molasses
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/105Aliphatic or alicyclic compounds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/24Compounds of alkaline earth metals, e.g. magnesium
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/10Feeding-stuffs specially adapted for particular animals for ruminants
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/30Feeding-stuffs specially adapted for particular animals for swines
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/70Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75Feeding-stuffs specially adapted for particular animals for birds for poultry
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/113Acidophilus
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

Abstract

The invention relates to a method for preparing high dietary fiber fermented feed by utilizing pineapple peel residues and application thereof. The high dietary fiber fermented feed is prepared by anaerobic fermentation of 83.75-91.74 parts of pineapple peel residues, 7.80-15.97 parts of microbial leavening agent and 0-0.46 part of sodium carbonate serving as pH value regulator; the microbial starter comprises the following components: 0.07-7.34 parts of lactobacillus acidophilus, 0.0004-0.05 part of saccharomyces cerevisiae, 0-7.33 parts of molasses, 0-1.69 parts of urea and 0.42-0.84 part of calcium carbonate. The method fully utilizes the pineapple residue resources, increases the functions and the comprehensive additional value of the pineapple residue byproduct product, and the fermented pineapple residue product obtained by adopting the fermentation process has fragrant and mellow smell, stable quality and uniform quality; it is rich in high dietary fiber, beneficial viable count or organic acid, and greatly improves palatability and animal intestinal health; the nutritive value, the feeding value and the utilization rate of the pineapple residue are greatly improved, and the feed cost is reduced; makes full use of unconventional feed resources, prevents environmental pollution and promotes the sustainable development of the breeding industry.

Description

Method for preparing high-dietary-fiber fermented feed by using pineapple peel residues and application of high-dietary-fiber fermented feed
Technical Field
The invention relates to the technical field of feeds, and particularly relates to a method for preparing a high-dietary-fiber fermented feed by using pineapple peel residues and application of the high-dietary-fiber fermented feed.
Technical Field
China is one of ten major countries of pineapples, the pineapple yield of China reaches millions of tons every year, the pineapple pomace accounts for 50-60% of the whole pineapple, but the utilization amount of the pineapple peel pomace generated after the pineapples are processed is extremely limited, the pineapple peel pomace is not used as an animal auxiliary feed or used for extracting bromelain and pectin, and is not used as a waste material but used in a high-valued manner, so that resources are wasted, and serious environmental pollution is caused. The pineapple peel residue has high nutritive value and good palatability, is popular among cattle, sheep and pigs, has higher feeding value and unique pharmaceutical function of bromelain, and is the best raw material of animal feed. However, because the pineapple dregs are easy to go bad and rot, the problems that the safety of the meat milk product is influenced by directly feeding residual ripener, pesticide and the like on the peels without treatment and the like are solved, and the use of the pineapple dregs is limited. Therefore, if the corresponding technology is adopted for reasonable processing, the maximum utilization of resource utilization can be realized, and the feeding value of the feed is improved.
The dietary fiber is a high molecular polysaccharide which is difficult to digest by digestive enzymes of human bodies, and has various generally accepted physiological functions, for example, the dietary fiber diet can solve some problems of piglet diarrhea, sow constipation and the like. At present, the development and application of food dietary fibers (beet fibers, wheat bran, corn bran, oat fibers, soybean residue fibers and the like) in China are wide, but the research and development of fruit dietary fibers are relatively few, and the fruit dietary fibers comprise pineapple peel residues. The pineapple peel residue is rich in dietary fiber, rich in sugar and vitamins and good in water retention and fermentability. If the pineapple peel residue is fermented by the microbial fermentation technology to produce high-quality dietary fiber products and the like, the nutritional value and the feeding value of the pineapple peel residue can be improved, and the effects of energy conservation and emission reduction are achieved.
At present, experts at home and abroad have carried out certain research on the development and utilization of pineapple pomace, and the silage for cattle and sheep is mainly prepared by a silage technology, and protein feed, probiotic preparation, active dry yeast or other biological feed and the like for single stomach and ruminant are prepared by a fermentation technology. The prior art has the problems of complex fermentation process, high energy consumption and processing cost, loss of beneficial nutritional ingredients in the processing process or unstable product quality and the like, and the research on preparing the pineapple dietary fiber by the fermentation technology is relatively less. In the research aspect of preparing dietary fiber from pineapple peel residues, the publication number is CN102246966A, namely the patent application of 'preparation method of pineapple dietary fiber', the dietary fiber is prepared from pineapple residues serving as raw materials by the working procedures of alkali treatment, alkali washing, pulping to remove black spots, acid regulation, filter pressing, sterilization and the like. The patent application utilizes pineapple dregs for processing, the adopted process is more basic, and a microbial fermentation technology is not adopted, so that the obtained product is more primary. Therefore, a large digging space exists for the development and utilization of the pineapple peel residues. If the high-quality dietary fiber products and the like are prepared by fermenting the pineapple peel residues by using the microbial fermentation technology, the aims of improving the product quality, reducing the product cost, protecting the environment and turning waste into wealth can be fulfilled.
Disclosure of Invention
In order to solve the problems, the invention provides a method for preparing high dietary fiber fermented feed by using pineapple peel residues and application thereof. The research aims to provide a comprehensive utilization method of pineapple peel residues through a microbial anaerobic fermentation technology, and aims to solve the limitation of industrial utilization of the pineapple peel residues in the prior art, fully convert the pineapple peel residues into usable substances, improve the feeding value of the pineapple peel residues and avoid pollution of waste pineapple peels to the environment.
The method for preparing the high dietary fiber fermented feed by using the pineapple peel residues, provided by the invention, comprises the steps of 83.75-91.74 parts of the pineapple peel residues, 7.80-15.97 parts of a microbial leavening agent and 0-0.46 part of a pH value regulator sodium carbonate, and performing anaerobic fermentation to obtain the high dietary fiber fermented feed; the microbial starter comprises the following components: 0.07-7.34 parts of lactobacillus acidophilus, 0.0004-0.05 part of saccharomyces cerevisiae, 0-7.33 parts of molasses, 0-1.69 parts of urea and 0.42-0.84 part of calcium carbonate.
The method for preparing the high dietary fiber fermented feed by using the pineapple peel residues comprises the following steps:
1) activating lactobacillus acidophilus, performing amplification culture for 19-21h to obtain lactobacillus acidophilus liquid, mixing lactobacillus acidophilus liquid and saccharomyces cerevisiae powder in proportion to obtain composite microbial liquid, and adding molasses, urea and calcium carbonate into the composite microbial liquid in proportion to be mixed uniformly to obtain a microbial starter for later use;
2) pulping the pineapple peel residue at 4-10 ℃ overnight to obtain pineapple peel residue pulp, inoculating the microbial starter into the pineapple peel residue pulp, uniformly mixing, adding sodium carbonate to adjust the pH to 5.48-6.48 to obtain the pineapple peel residue pulp to be fermented, and placing the pineapple peel residue pulp in an environment at 25-30 ℃;
3) squeezing out the water in the pineapple peel residue pulp to be fermented by using a filter press to obtain pineapple peel juice to be fermented and pineapple peel residue to be fermented, wherein the water content of the pineapple peel residue juice to be fermented is 70-75%;
4) filling the pineapple residue juice to be fermented into a fermentation barrel, sealing and fermenting, 25-40 kg-
Barrel, standing and fermenting for 2-5 days at 30-35 ℃ to obtain fermented pineapple dreg juice;
5) and (3) putting the pineapple peel residues to be fermented into a fermentation breather bag with a breather hole for fermentation, wherein the fermentation breather bag is 20-30 kg/bag, and standing and fermenting at the temperature of 30-35 ℃ for 2-5 days to obtain the fermented pineapple peel residues.
Further, the fermentation in the steps 4) and 5) is anaerobic fermentation.
Further, the fermented pineapple dreg juice obtained in the step 4) is high-acid ferment, and is diluted by 300 times during ensiling and then used for spraying ensiling raw materials.
Further, the fermented pineapple peel residue obtained in the step 5) is high dietary fiber fermented feed, and concentrated feed is replaced by 10-40% in ruminant breeding.
Further, the fermented pineapple peel residue obtained in the step 5) is high dietary fiber fermented feed, and is used for replacing complete feed in a proportion of 5-20% in the feeding of sows or laying hens.
The invention has the following beneficial effects:
1. the invention fully utilizes the pineapple residue resources, increases the functions and the comprehensive additional value of the byproduct product of the pineapple residue, effectively improves the nutritional value of the pineapple peel residue fermented feed, improves the quality of dietary fiber and reduces the production cost.
2. The preparation process has strong controllability, high efficiency and short fermentation period, and can prevent mixed bacteria pollution; the microbial conversion produces some flavor substances, improves the palatability of the feed, and can greatly improve the content level and the fermentation depth of active substances such as the number of beneficial living bacteria, organic acid and the like, thereby improving the nutritional value and the utilization value of the feed.
3. The invention uses the common additives of the fermented feed allowed in the feed additive catalog (2013) for fermentation of fermented pineapple peel residues, the strain combination of the common additives is mainly composed of lactobacillus acidophilus and saccharomyces cerevisiae, the method for fermenting pineapple peel residues by the microbial fermentation technology to obtain high-quality byproducts fills the blank of the technology for preparing dietary fibers by fermenting pineapple peel residues by microorganisms.
4. The lactobacillus acidophilus selected by the invention can generate acidic substances such as lactic acid, acetic acid and the like or antibiotics and the like in microbial fermentation, can inhibit the growth of mixed bacteria, improve the total acid content in the fermented pineapple, reduce the pH value of the fermented pineapple, and achieve the purpose of improving the smell and the palatability of the fermented feed.
5. The saccharomyces cerevisiae selected by the invention can be rapidly proliferated in the pineapple peel residue substrate added with molasses, and the monosaccharide is decomposed into alcohol, so that the smell of the fermentation material is improved, and the feeding attraction of the feed is improved.
6. According to the embodiment of the invention, the pineapple peel residue is fermented by adopting an anaerobic fermentation technology, namely the pineapple peel residue is inoculated with lactobacillus acidophilus and saccharomyces cerevisiae, and a carbon source, a nitrogen source or an acid-base regulator is supplemented for fermentation, so that the total acid level and the fermentation depth of the pineapple peel residue are improved, the quality of dietary fibers is improved, and the fermentation benefit of the pineapple residue is improved. The fermented pineapple dregs adopt an anaerobic fermentation mode, can inhibit the growth of mixed bacteria, promote the proliferation of lactobacillus acidophilus and the like and the generation of metabolites thereof, and increase the food calling property and the palatability.
7. The production process for preparing the fermented pineapple residue product in the preferred embodiment of the invention is easy to operate, high in efficiency, short in fermentation time and strong in controllability. The fermented pineapple residue product obtained by the fermentation process has fragrant smell, stable quality and uniform quality; it is rich in high dietary fiber, beneficial viable count or organic acid, and greatly improves palatability and animal intestinal health; the nutritive value, the feeding value and the utilization rate of the pineapple residue are greatly improved, and the feed cost is reduced; makes full use of unconventional feed resources, prevents environmental pollution and promotes the sustainable development of the breeding industry.
Detailed Description
The present invention is further illustrated by the following examples.
Example 1 preparation of high dietary fiber fermented feed A from pineapple bran
The high dietary fiber fermented feed A is prepared by carrying out anaerobic fermentation on 84.03 parts of pineapple peel residues, 15.55 parts of a microbial starter and 0.42 part of a pH value regulator sodium carbonate; the microbial starter comprises the following components: 6.72 parts of lactobacillus acidophilus, 0.0004 part of saccharomyces cerevisiae, 6.72 parts of molasses, 1.68 parts of urea and 0.42 part of calcium carbonate. The preparation method comprises the following steps:
1) activating lactobacillus acidophilus, performing amplification culture for 20h to obtain lactobacillus acidophilus liquid, mixing lactobacillus acidophilus liquid and saccharomyces cerevisiae powder in proportion to obtain composite microbial liquid, and adding molasses, urea and calcium carbonate into the composite microbial liquid in proportion to be mixed uniformly to obtain a microbial starter for later use;
2) pulping the pineapple peel residue at 4 ℃ overnight to obtain pineapple peel residue pulp, inoculating the microbial starter into the pineapple peel residue pulp, uniformly mixing, adding sodium carbonate to adjust the pH value to 5.48 to obtain the pineapple peel residue pulp to be fermented, and placing the pineapple peel residue pulp in an environment at 30 ℃;
3) squeezing out the water in the pineapple peel residue pulp to be fermented by using a filter press to obtain pineapple residue juice to be fermented and pineapple peel residue to be fermented, wherein the water content of the pineapple peel residue juice to be fermented is 75%;
4) filling the pineapple residue juice to be fermented into a fermentation barrel, sealing and fermenting at 30 kg/barrel, standing and fermenting at 30 ℃ for 3 days to obtain fermented pineapple residue juice;
5) and (3) putting the pineapple peel residues to be fermented into a fermentation breather bag with a breather hole for fermentation, wherein the fermentation breather bag is 30 kg/bag, and standing and fermenting at 30 ℃ for 3 days to obtain the fermented pineapple peel residues.
Example 2 preparation of high dietary fiber fermented feed B from pineapple bran
The high dietary fiber fermented feed B is prepared by 84.39 parts of pineapple peel residues, 15.19 parts of a microbial starter culture and 0.42 part of a pH value regulator sodium carbonate through anaerobic fermentation; the microbial starter comprises the following components: 6.75 parts of lactobacillus acidophilus, 0.0004 part of saccharomyces cerevisiae, 6.75 parts of molasses, 1.69 parts of urea and 0.42 part of calcium carbonate.
The preparation method is the same as example 1, and the pH value in step 2) is pH 5.48.
Example 3 preparation of high dietary fiber fermented feed C from pineapple bran
The high dietary fiber fermented feed C is prepared by carrying out anaerobic fermentation on 84.03 parts of pineapple peel residues, 15.55 parts of a microbial starter and 0.42 part of a pH value regulator sodium carbonate; the microbial starter comprises the following components: 6.72 parts of lactobacillus acidophilus, 0.0004 part of saccharomyces cerevisiae, 6.75 parts of molasses, 1.68 parts of urea and 0.84 part of calcium carbonate.
The preparation method is the same as example 1, except that:
the pH value in the step 2) is 5.9;
the water content of the pineapple peel residue to be fermented in the step 3) is 70 percent;
step 4), filling the pineapple dreg juice into a fermentation barrel, sealing and fermenting the pineapple dreg juice at 25 kg/barrel, standing and fermenting the pineapple dreg juice at 30 ℃ for 4 days to obtain fermented pineapple dreg juice;
and 5) putting the pineapple peel residues into a fermentation breather bag with a breather hole for fermentation, wherein the fermentation breather bag is 25 kg/bag, and standing and fermenting at 30 ℃ for 4 days to obtain the fermented pineapple peel residues.
Example 4 preparation of high dietary fiber fermented feed D from pineapple bran
The high dietary fiber fermented feed D is prepared by 83.89 parts of pineapple peel residues, 15.94 parts of microbial leavening agent and 0.17 part of sodium carbonate serving as pH value regulator through anaerobic fermentation; the microbial starter comprises the following components: 6.71 parts of lactobacillus acidophilus, 0.0004 part of saccharomyces cerevisiae, 6.71 parts of molasses, 1.68 parts of urea and 0.84 part of calcium carbonate.
The preparation method is the same as example 1, except that:
the pH value in the step 2) is 6.14;
the water content of the pineapple peel residue to be fermented in the step 3) is 70 percent;
putting the pineapple dreg juice into a fermentation barrel in the step 4), sealing and fermenting the pineapple dreg juice, wherein the fermentation barrel is 25 kg/barrel, and standing and fermenting the pineapple dreg juice at 35 ℃ for 2 days to obtain fermented pineapple dreg juice;
and 5) putting the pineapple peel residues into a fermentation breather bag with a breather hole for fermentation, wherein the fermentation breather bag is 25 kg/bag, and standing and fermenting at 35 ℃ for 2 days to obtain the fermented pineapple peel residues.
Example 5 preparation of high dietary fiber fermented feed E from pineapple bran
The high dietary fiber fermented feed E is prepared by 83.75 parts of pineapple peel residues, 15.91 parts of a microbial starter culture and 0.34 part of a pH value regulator sodium carbonate through anaerobic fermentation; the microbial starter comprises the following components: 6.70 parts of lactobacillus acidophilus, 0.0004 part of saccharomyces cerevisiae, 6.70 parts of molasses, 1.68 parts of urea and 0.84 part of calcium carbonate.
The preparation method is the same as example 1, except that:
the pH value in the step 2) is pH 6.48;
the water content of the pineapple peel residues to be fermented in the step 3) is 72 percent;
putting the pineapple dreg juice into a fermentation barrel in the step 4), sealing and fermenting the pineapple dreg juice, wherein the fermentation barrel is 35 kg/barrel, and standing and fermenting the pineapple dreg juice at 35 ℃ for 3 days to obtain fermented pineapple dreg juice;
and 5) putting the pineapple peel residues into a fermentation breather bag with a breather hole for fermentation, wherein the fermentation breather bag is 35 kg/bag, and standing and fermenting at 35 ℃ for 3 days to obtain the fermented pineapple peel residues.
Example 6 preparation of high dietary fiber fermented feed F from pineapple bran
The high dietary fiber fermented feed F is prepared by carrying out anaerobic fermentation on 91.74 parts of pineapple peel residues, 7.80 parts of microbial leavening agent and 0.46 part of sodium carbonate serving as pH value regulator; the microbial leavening agent comprises the following components: 7.34 parts of lactobacillus acidophilus, 0.0005 part of saccharomyces cerevisiae, 6.70 parts of molasses, 1.68 parts of urea and 0.84 part of calcium carbonate.
The preparation method is the same as example 1, except that:
the pH value in the step 2) is 6.70;
the water content of the pineapple peel residue to be fermented in the step 3) is 75 percent;
putting the pineapple dreg juice into a fermentation barrel in the step 4), sealing and fermenting the pineapple dreg juice, wherein the fermentation barrel is 35 kg/barrel, and standing and fermenting the pineapple dreg juice at 35 ℃ for 5 days to obtain fermented pineapple dreg juice;
and 5) putting the pineapple peel residues into a fermentation breather bag with a breather hole for fermentation, wherein the fermentation breather bag is 35 kg/bag, and standing and fermenting at 35 ℃ for 5 days to obtain the fermented pineapple peel residues.
Example 7 preparation of high dietary fiber fermented feed G from pineapple bran
The high dietary fiber fermented feed G is prepared by carrying out anaerobic fermentation on 85.47 parts of pineapple peel residues, 14.07 parts of a microbial starter culture and 0.46 part of a pH value regulator sodium carbonate; the microbial leavening agent comprises the following components: 6.84 parts of lactobacillus acidophilus, 0.0004 part of saccharomyces cerevisiae, 6.70 parts of molasses, 1.68 parts of urea and 0.84 part of calcium carbonate.
The preparation method is the same as example 1, except that:
the pH value in the step 2) is 6.38;
the water content of the pineapple peel residue to be fermented in the step 3) is 75 percent;
step 4), filling the pineapple dreg juice into a fermentation barrel, sealing and fermenting the pineapple dreg juice at 35 kg/barrel, standing and fermenting the pineapple dreg juice at 35 ℃ for 4 days to obtain fermented pineapple dreg juice;
and 5) putting the pineapple peel residues into a fermentation breather bag with a breather hole for fermentation, wherein the fermentation breather bag is 35 kg/bag, and standing and fermenting for 4 days at 35 ℃ to obtain the fermented pineapple peel residues.
TABLE 1 detection indexes of fermented pineapple peel residue and pineapple residue juice in examples
Figure BDA0003569728950000101
As can be seen from Table 1, under the parameter conditions provided by the scheme of the invention, the detection index results of the fermented pineapple peel residues obtained by fermentation in the invention cases are better. The fermented pineapple peel residues prepared under the parameter conditions provided by the scheme of the invention have good stability. Compared with untreated raw materials, the fermented pineapple peel residue obtained by the scheme of the invention is rich in various active substances such as beneficial viable count, various organic acids and the like, such as obviously improving the total acid content and increasing the food calling property and palatability of the feed; the quality of the fermented pineapple bran dietary fiber product is greatly improved, the nutritive value and the feeding value of the pineapple bran are improved, the feed cost is reduced, the fermented pineapple bran dietary fiber product can be used as a good dietary fiber fermented feed, and the utilization rate of pineapple byproducts is improved.
The fermented pineapple peel residue is high dietary fiber fermented feed, concentrated feed can be replaced by 10-40% of the fermented pineapple peel residue in ruminant breeding, and high-quality dietary fiber and energy sources are provided for ruminant rumen microorganisms.
The fermented pineapple peel residue is high dietary fiber fermented feed, can replace complete feed by 5-20% in the feeding of sows or laying hens, provides high-quality dietary fiber for monogastric animals, and improves the intestinal health state of the monogastric animals fed in an intensive manner.
The fermented pineapple dreg juice is high-acid ferment, and is diluted by 300 times during ensiling to be used for spraying ensiling raw materials, so that ensiling fermentation speed can be increased, and ensiling mildew can be reduced.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims (6)

1. A method for preparing high dietary fiber fermented feed by using pineapple peel residues is characterized in that the high dietary fiber fermented feed is prepared by 83.75-91.74 parts of pineapple peel residues, 7.80-15.97 parts of microbial leavening agents and 0-0.46 part of sodium carbonate serving as pH value regulators through anaerobic fermentation; the microbial starter comprises the following components: 0.07-7.34 parts of lactobacillus acidophilus, 0.0004-0.05 part of saccharomyces cerevisiae, 0-7.33 parts of molasses, 0-1.69 parts of urea and 0.42-0.84 part of calcium carbonate.
2. The method for preparing the high dietary fiber fermented feed by using the pineapple peel residues, according to claim 1, is characterized by comprising the following steps:
1) activating lactobacillus acidophilus, performing amplification culture for 19-21h to obtain lactobacillus acidophilus liquid, mixing lactobacillus acidophilus liquid and saccharomyces cerevisiae powder in proportion to obtain composite microbial liquid, and adding molasses, urea and calcium carbonate into the composite microbial liquid in proportion to be mixed uniformly to obtain a microbial starter for later use;
2) pulping the pineapple peel residue at 4-10 ℃ overnight to obtain pineapple peel residue pulp, inoculating the microbial starter into the pineapple peel residue pulp, uniformly mixing, adding sodium carbonate to adjust the pH to 5.48-6.48 to obtain the pineapple peel residue pulp to be fermented, and placing the pineapple peel residue pulp in an environment at 25-30 ℃;
3) squeezing out the water in the pineapple peel residue pulp to be fermented by using a filter press to obtain pineapple peel juice to be fermented and pineapple peel residue to be fermented with the water content of 70-75%;
4) filling the pineapple residue juice to be fermented into a fermentation barrel, sealing and fermenting the pineapple residue juice at 25-40 kg/barrel, standing and fermenting the pineapple residue juice at 30-35 ℃ for 2-5 days to obtain fermented pineapple residue juice;
5) and (3) putting the pineapple peel residues to be fermented into a fermentation breather bag with a breather hole for fermentation, wherein the fermentation breather bag is 20-30 kg/bag, and standing and fermenting at the temperature of 30-35 ℃ for 2-5 days to obtain the fermented pineapple peel residues.
3. The method for preparing a high dietary fiber fermented feed by using pineapple peel residues as claimed in claim 2, wherein the fermentation in steps 4) and 5) is anaerobic fermentation.
4. The method for preparing a high dietary fiber fermented feed by using pineapple peel residues as claimed in claim 2, wherein the fermented pineapple peel juice obtained in step 4) is high acid ferment, and is diluted by 300 times for spraying silage raw material during ensiling.
5. The method for preparing a high dietary fiber fermented feed using pineapple peel pomace according to claim 2, wherein the fermented pineapple peel pomace obtained in the step 5) is a high dietary fiber fermented feed, and the concentrated feed is replaced by 10-40% in feeding ruminant animals.
6. The method for preparing a high dietary fiber fermented feed by using pineapple peel residues as claimed in claim 2, wherein the fermented pineapple peel residues obtained in step 5) are high dietary fiber fermented feeds, and are used for replacing complete feed in a proportion of 5-20% in the feeding of sows or laying hens.
CN202210321117.4A 2022-03-29 2022-03-29 Method for preparing high-dietary-fiber fermented feed by using pineapple peel residues and application of high-dietary-fiber fermented feed Pending CN114651895A (en)

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CN101617737A (en) * 2009-07-31 2010-01-06 广州市希普生物饲料有限公司 Method for using pineapple peel and slag to prepare high activity dried yeast and ferment feed
CN102150746A (en) * 2010-12-28 2011-08-17 广东海洋大学 Packaged and fermented pineapple dreg feed and energy-saving processing method thereof
CN104472865A (en) * 2014-11-29 2015-04-01 吴成文 Method for preparing silage
CN104544111A (en) * 2014-05-22 2015-04-29 林坤华 Method for reusing pineapple peel
CN106819408A (en) * 2017-03-06 2017-06-13 中国林业科学研究院林业研究所 Aroma type Chinese toon biological feedstuff and preparation method thereof
CN107889954A (en) * 2017-11-28 2018-04-10 佛山宝来迪生物科技有限公司 A kind of pineapple peel viable bacteria feedstuff for live pigs and its preparation and feeding method
CN107981046A (en) * 2017-11-28 2018-05-04 佛山宝来迪生物科技有限公司 A kind of method of comprehensive utilization of pineapple peel
CN114158648A (en) * 2021-11-09 2022-03-11 新疆冠农果茸股份有限公司 Tomato peel residue biological fermentation feed, preparation method and application thereof

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101617737A (en) * 2009-07-31 2010-01-06 广州市希普生物饲料有限公司 Method for using pineapple peel and slag to prepare high activity dried yeast and ferment feed
CN102150746A (en) * 2010-12-28 2011-08-17 广东海洋大学 Packaged and fermented pineapple dreg feed and energy-saving processing method thereof
CN104544111A (en) * 2014-05-22 2015-04-29 林坤华 Method for reusing pineapple peel
CN104472865A (en) * 2014-11-29 2015-04-01 吴成文 Method for preparing silage
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CN114158648A (en) * 2021-11-09 2022-03-11 新疆冠农果茸股份有限公司 Tomato peel residue biological fermentation feed, preparation method and application thereof

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