CN114642625A

CN114642625A - Whitening and moisturizing traditional Chinese medicine composition and preparation method and application thereof

Info

Publication number: CN114642625A
Application number: CN202210146160.1A
Authority: CN
Inventors: 孙怀庆; 曾令椿; 孙云起; 郭朝万; 陈杰
Original assignee: Guangdong Marubi Biological Technology Co Ltd
Current assignee: Guangdong Marubi Biological Technology Co Ltd
Priority date: 2022-02-17
Filing date: 2022-02-17
Publication date: 2022-06-21
Anticipated expiration: 2042-02-17
Also published as: CN114642625B

Abstract

The invention discloses a whitening and moisturizing traditional Chinese medicine composition as well as a preparation method and application thereof, wherein the composition comprises the following raw materials: red wine, cherry plum peel extract, cherry fruit extract, cherry plum kernel extract, rhizoma Atractylodis Macrocephalae, Poria, rhizoma Bletillae, Bombyx Batryticatus, radix Paeoniae alba, cortex Phellodendri, Galla chinensis, fructus Cnidii, semen Coicis, and salicylic acid. Has effects of whitening skin, keeping moisture and removing speckle, and has no toxicity and photosensitivity. The preparation method of the composition comprises the following steps: firstly, grinding bighead atractylodes rhizome, white poria, bletilla striata, stiff silkworm, white paeony root, golden cypress, gallnut, common cnidium fruit and coix seed into powder to obtain traditional Chinese medicine powder; mixing the Chinese medicinal powder with red wine, stirring, adding Prunus cerasifera pericarp extract, Prunus cerasifera fruit extract and Prunus cerasifera seed extract, mixing, fermenting to obtain fermented product, and adding salicylic acid. The fermented composition has stronger penetrability and improves the effects of moistening, repairing and relieving. The composition is applied to facial masks, essence, toner and cream.

Description

Whitening and moisturizing traditional Chinese medicine composition and preparation method and application thereof

Technical Field

The invention relates to the technical field of whitening skin care products, in particular to a whitening and moisturizing traditional Chinese medicine composition and a preparation method and application thereof.

Background

At present, the most applied whitening methods in modern science and technology are two, the first method is to adopt vitamin C and derivatives thereof, the substances have reducibility and can reduce the generation of oxidation free radicals so as to reduce the generation of tyrosinase, but the substances have photosensitivity and cannot be used in the daytime; another class of substances are resorcinol-containing compounds, such as arbutin, ferulic acid, and the like, which are easily oxidatively decomposed by light and are not suitable for use in daytime whitening creams. The seven-seed white mask is a long-circulating beauty formula in China, and consists of seven Chinese medicaments with white characters, which are respectively as follows: radix angelicae, radix paeoniae alba, bletilla striata, rhizoma typhonii, poria alba, rhizoma atractylodis macrocephalae and radix ampelopsis, but seven rhizoma typhonii are toxic, and radix angelicae is photosensitive.

With the rapid expansion of the planting area of cherry plum in China, the deep processing field of cherry plum is continuously expanded. A large amount of cherry plums are produced in the cherry deep processing process of cherry juice, wine, cans, preserved fruits and the like, and most of the cherry plums are used for feed production or waste, so that waste and resource loss are caused.

Disclosure of Invention

In order to overcome the defects of the prior art, one of the purposes of the invention is to provide a whitening and moisturizing traditional Chinese medicine composition, wherein cherry plum peel extract, cherry fruit extract and cherry plum kernel extract are supplemented with traditional Chinese medicines with whitening function, so that the whitening and moisturizing effect is achieved, and no photosensitivity exists; the invention also aims to provide a preparation method of the whitening and moisturizing traditional Chinese medicine composition, which comprises the steps of mixing the components, fermenting, adding salicylic acid, and improving the permeability; the invention also aims to provide the application of the whitening and moisturizing traditional Chinese medicine composition, and the composition can be used as one of raw materials of masks, essence, toner and face cream, and is wide in application.

One of the purposes of the invention is realized by adopting the following technical scheme:

a whitening and moisturizing traditional Chinese medicine composition comprises the following raw materials:

red wine, cherry plum peel extract, cherry fruit extract, cherry plum kernel extract, rhizoma Atractylodis Macrocephalae, Poria, rhizoma Bletillae, Bombyx Batryticatus, radix Paeoniae alba, cortex Phellodendri, Galla chinensis, fructus Cnidii, semen Coicis, and salicylic acid.

Further, the feed comprises the following raw materials in parts by mass:

50-60 parts of red wine, 10-15 parts of cherry plum peel extract, 10-20 parts of cherry fruit extract, 10-20 parts of cherry plum kernel extract, 10-20 parts of bighead atractylodes rhizome, 10-20 parts of white poria, 10-15 parts of bletilla striata, 5-10 parts of stiff silkworm, 10-20 parts of white paeony root, 5-10 parts of golden cypress, 5-10 parts of Chinese gall, 5-10 parts of fructus cnidii, 5-10 parts of semen coicis and 0.1-0.2 part of salicylic acid. Wherein the cherry fruit extract is obtained by pulping cherry plum pulp, vacuum drying, and pulverizing. The cherry plum kernel extract is powder obtained by pulverizing and grinding cherry stone.

Specifically, the efficacy of each ingredient is as follows:

white atractylodes rhizome: a plant of Atractylodes of Compositae is used as medicine. Invigorating spleen, invigorating qi, eliminating dampness, promoting diuresis, arresting sweating, and preventing miscarriage. The rhizome contains volatile oil, and contains various alcohols (including various whitening components) such as alpha-humulene, beta-elemene, alpha-curcumene, 3 beta-acetoxy atractylone, and the like, and also contains various amino acids. Has effects in whitening skin, removing speckle, and promoting urination; reducing blood sugar; anticoagulant effect; anti-tumor effect; has antibacterial effect and can promote hematopoiesis.

White poria: the collagen has the effect of promoting proliferation of integrin and the like, the integrin can reflect the proliferation condition of fibroblasts and the adhesion condition between cells and between fibrin, and the proliferation of the integrin can reduce the diameter and the volume of collagen wrapped by the fibroblasts, so that the collagen has a contraction effect and can be used for skin tightening and fine line cosmetics; has antibacterial property, and has good inhibitory effect on various bacteria and fungi; it also has anti-inflammatory and moisture keeping effects.

Bletilla striata: the composition has an inhibiting effect on 5 a-reductase, shows that the composition has a good effect of preventing and treating alopecia caused by high androgen, and can be used for hair growth and acne products; the bletilla striata extract is also an antioxidant, a skin whitening agent and an anti-inflammatory agent with obvious effects.

Silkworm larva: bombyx Batryticatus is Bombycidae. Before silking, the larva of the insect silkworm is infected with beauveria bassiana to cause death. The herbs for external use are salty and pungent in flavor and neutral in nature. Can be used for treating facial dark and diseases of facial paralysis. Title of compendium of materia Medica: the white morning glory (white morning glory seed, white morning glory, ugly and cattle also named after the old world) is added with the white silkworm powder, the water and the mixture. Bombyx Batryticatus contains amino acids and active mercerizing agent, and has effects of nourishing skin and caring skin. The protein contained in the stiff silkworm stimulates the epithelial gland and regulates the secretion of sex hormone, so that the stiff silkworm has certain curative effect on chloasma caused by female sexual hormone secretion disorder. The Bombyx Batryticatus contains vitamin E9.89%, and has effects of scavenging free radicals and resisting senile plaque formed by oxidation of lipid. The active mercerizing agent can promote the regeneration of skin cells, regulate sebum, improve skin microcirculation, whiten and prevent sunburn, eliminate pigmentation and keep skin elasticity.

Radix Paeoniae alba is sweet and sour in flavor and slightly cold in nature, has the effect of nourishing blood, and can be used for treating sallow complexion and facial mottle.

Phellodendron bark: is a yellow powder extract product of bark of phellodendron amurense belonging to Rutaceae. Mainly contains berberine, and contains palmatine, phellodendrine, dauricine, sinomenine, phellodendron bark, etc. Can promote the generation of collagen, and has the function of anti-aging by combining the oxidation resistance; can inhibit hair growth, and can be used for treating skin hirsutism in combination with aromatase activation; has anti-inflammatory and odor inhibiting effects.

Gallnut: has effects in nourishing and moistening skin, protecting skin, relieving inflammation, inhibiting bacteria, whitening skin, nourishing hair, blackening hair, preventing corrosion and oxidation, perfuming skin, and regulating color. The main functions are grease-resistant oxidant and antibacterial agent.

Fructus cnidii: is a plant of annual herbaceous plant of Umbelliferae, and has antifungal effect; has good efficacy for treating acute exudative dermatosis; it also has skin nourishing effect. Mainly used as anti-inflammatory agent and skin conditioner.

Coix seed: has moderate antioxidant effect, and can increase skin activity, reduce wrinkle, and resist aging; can reduce skin oil wax property and improve skin softness; has good decomposition effect on fat cells, and can be used for weight-reducing products; has effects in inhibiting tyrosinase and melanocyte, whitening skin, and removing speckle.

Salicylic acid: salicylic acid is the most major component in non-prescription acne treatment, and salicylic acid is the major functional component in a large number of classical acne removal products. Salicylic acid is oil-soluble, can permeate into pores rich in lipid, removes acne dirt accumulated in pores, loosens acne plugs, plays a certain anti-inflammatory role, is an effective component for treating acne, and can be used in combination with a cleanser, a moisturizer and a cream. When the salicylic acid is used at a low concentration (1% -2%) and a medium concentration (5% -10%), it has keratolytic effect, and can whiten and tenderize skin when applied to the skin.

The cherry plum fruits also contain active substances such as organic acids, amino acids, anthocyanins, polyphenols, trace elements and the like, and the content of the anthocyanins in the cherry plum fruits is the highest especially in the cherry plum peels. The anthocyanin is a natural plant component and is used for preparing the facial mask to clean redundant horny layers and free radicals on the face, so that the skin can be more white, bright and smooth; in addition, the anthocyanin has the effects of sterilizing and diminishing inflammation, and the long-term application of the anthocyanin mask can adjust the water-oil balance of the skin, improve the skin sensitivity problems such as acne and the like, reduce the occurrence of acne and comedo and fade acne marks; meanwhile, the anthocyanin has good oxidation resistance and can also help to play a role in resisting aging and smoothing fine wrinkles. The main components of cherry pit include water, flavonoids, protein, volatile oil, etc. Also contains antioxidant substances such as anthocyanidin, vitamin C, carotene, vitamin E, selenium, and brass, and is helpful for resisting oxidation of free radicals. Particularly, the cherry plum kernel extract takes cherry kernels produced in the production of cherry wine as raw materials. The kernel outing rate of cherry stone can reach more than 20 percent, and the content of unsaturated fatty acid in cherry oil is very high, thus having higher nutritive value.

Further, the preparation method of the cherry plum peel extract comprises the following steps:

1) collecting cherry peel, pulping, and performing vacuum freeze drying and crushing pretreatment to obtain cherry peel powder;

2) adding the cherry peel powder into ethanol, and performing ultrasonic extraction to obtain a crude extract;

3) and purifying and eluting the crude extract by adopting macroporous resin to obtain the cherry plum peel extract.

Further, the vacuum freeze-drying conditions are as follows: the temperature is-78 to-70 ℃, the vacuum degree is 15 to 20Pa, and the drying time is 40 to 48 hours.

Still further, the conditions of purification elution are as follows: ethanol with the concentration of 70-80% is used as eluent, and the volume ratio of the crude extract to the macroporous resin column is 1: (3-20), the elution flow rate is 1-5 mL/min, and 3-7 column volumes are eluted.

The second purpose of the invention is realized by adopting the following technical scheme:

the preparation method of the whitening and moisturizing traditional Chinese medicine composition comprises the following steps:

1) grinding Atractylodis rhizoma, Poria, rhizoma Bletillae, Bombyx Batryticatus, radix Paeoniae alba, cortex Phellodendri, Galla chinensis, fructus Cnidii, and Coicis semen into powder to obtain Chinese medicinal powder;

2) mixing the Chinese medicinal powder with red wine, and stirring;

3) adding Prunus cerasifera pericarp extract, Prunus cerasifera fruit extract and Prunus cerasifera seed extract, mixing, and fermenting to obtain fermented product;

4) salicylic acid is added into the fermentation product to obtain the whitening and moisturizing traditional Chinese medicine composition.

Further, in the step 2), the mixing speed is 50-100 r/min, and the stirring time is 5-10 min.

Further, in the step 2), mixing at 50-70 ℃.

Further, in the step 3), fermenting for 2-5 days at the temperature of 32-37 ℃.

The third purpose of the invention is realized by adopting the following technical scheme:

the application of the whitening and moisturizing traditional Chinese medicine composition is applied to masks, essence, toning water and face cream.

Compared with the prior art, the invention has the beneficial effects that:

(1) the main components of the composition are cherry plum peel extract, cherry fruit extract, cherry plum kernel extract, bighead atractylodes rhizome, white poria, bletilla striata, stiff silkworm, white paeony root, amur corktree bark, Chinese gall, common cnidium fruit, coix seed and salicylic acid; the raw materials of the cherry plum peel extract, the cherry fruit extract and the cherry plum kernel extract are cherry plums, but the extracts of the peel, the pulp and the kernel are respectively added to better extract the active ingredients in the cherry plum fruit extract, and the raw materials of the cherry plum kernel extract are prepared by recovering waste kernels in wine brewing. The white atractylodes rhizome, the white poria, the bletilla striata, the stiff silkworm and the white paeony root are selected from the seven-seed white formula, and the coix seed and the golden cypress are taken as auxiliary materials, so that the white atractylodes rhizome, the white poria, the bletilla striata, the stiff silkworm and the white paeony root have the effects of whitening, moisturizing and removing freckles, and do not have toxicity and photosensitivity; the fructus cnidii has a good nourishing effect and can improve the moisturizing effect, and the salicylic acid can improve the permeability of the composition, but the irritation of the salicylic acid can be greatly reduced by compounding the fructus cnidii with the components. In addition, the composition of the invention takes red wine as a solvent, the red wine contains alcohol, the solubility of the traditional Chinese medicine components can be improved, and the red wine is also an antioxidant.

(2) The preparation method of the cherry plum peel extract serving as one of the raw materials of the composition comprises the following steps: the cherry plum peel is subjected to freeze drying and then extracted, an extracting solution is filtered and subjected to reduced pressure concentration, and then is purified by adopting macroporous resin, so that the extraction rate of the obtained anthocyanin extract is over 3 percent, the content of active ingredients in the anthocyanin extract is high, impurities are few, the content of flavone in the cherry plum peel extract is up to 40-70 percent, the cherry plum peel extract has good antioxidant effect and tyrosinase activity inhibition effect, the total antioxidant value is over 1.2mmol, the tyrosinase activity inhibition effect is over 85 percent, and the cherry plum peel extract is safe and reliable.

(3) The preparation method of the composition comprises the following steps: firstly, grinding bighead atractylodes rhizome, white poria, bletilla striata, stiff silkworm, white paeony root, golden cypress, gallnut, common cnidium fruit and coix seed into powder to obtain traditional Chinese medicine powder; mixing the Chinese medicinal powder with red wine, stirring, adding Prunus cerasifera pericarp extract, Prunus cerasifera fruit extract and Prunus cerasifera seed extract, mixing, fermenting to obtain fermented product, and adding salicylic acid. The fermented composition can change effective components into small molecules which are easier to be absorbed by the skin, the small molecules have stronger permeability, and stronger moistening, repairing and relieving effects can be naturally brought to the skin.

(4) The composition can be used as one of raw materials, is applied to masks, essence, toning water and creams, and has the advantages of definite effect, safe use and wide application range.

Detailed Description

The present invention is further described below with reference to specific embodiments, and it should be noted that, without conflict, any combination between the embodiments or technical features described below may form a new embodiment.

Example 1

A whitening and moisturizing traditional Chinese medicine composition comprises the following raw materials in parts by mass:

50 parts of red wine, 10 parts of cherry plum peel extract, 10 parts of cherry fruit extract, 10 parts of cherry plum kernel extract, 10 parts of bighead atractylodes rhizome, 10 parts of white poria, 10 parts of bletilla striata, 5 parts of stiff silkworm, 10 parts of white peony root, 5 parts of golden cypress, 5 parts of Chinese gall, 5 parts of fructus cnidii, 5 parts of semen coicis and 0.1 part of salicylic acid.

Specifically, the preparation method of the cherry plum peel extract comprises the following steps:

1) collecting cherry peel, pulping, and performing vacuum freeze drying and crushing pretreatment to obtain cherry peel powder; wherein the freezing temperature is-78 deg.C, the vacuum degree is 15Pa, and the drying time is 48 h.

2) Adding the cherry peel powder into the extracting solution, and carrying out ultrasonic extraction to obtain a crude extracting solution; wherein, the ultrasonic extraction conditions are as follows: the extract is acidified ethanol solution with material-liquid ratio of 1:5, extraction temperature of 30 deg.C, extraction time of 150min, ultrasonic power of 800W, ethanol concentration of acidified ethanol solution of 20%, and hydrochloric acid concentration of 0.05%.

3) Purifying and eluting the crude extract with DA201-C macroporous resin to obtain Prunus cerasifera pericarp extract. The conditions of the purification elution are as follows: ethanol with the concentration of 70-80% is used as eluent, and the volume ratio of the crude extract to the macroporous resin column is 1: (3-20), eluting at the flow rate of 1-5 mL/min for 3-7 column volumes.

2) heating the Chinese medicinal powder and red wine to 50 deg.C, mixing at 50r/min, and stirring for 10 min;

3) adding Prunus cerasifera pericarp extract, Prunus cerasifera fruit extract, and Prunus cerasifera seed extract, mixing, and fermenting at 32 deg.C for 2 days to obtain fermented product;

Example 2

60 parts of red wine, 15 parts of cherry plum peel extract, 20 parts of cherry fruit extract, 20 parts of cherry plum kernel extract, 20 parts of bighead atractylodes rhizome, 20 parts of white poria, 15 parts of bletilla striata, 10 parts of stiff silkworm, 20 parts of white peony root, 10 parts of golden cypress, 10 parts of Chinese gall, 10 parts of fructus cnidii, 10 parts of semen coicis and 0.2 part of salicylic acid.

1) collecting cherry peel, pulping, and performing vacuum freeze drying and crushing pretreatment to obtain cherry peel powder; the vacuum freeze-drying conditions are as follows: the temperature is-70 ℃, the vacuum degree is 20Pa, and the drying time is 40 h.

2) Adding the cherry peel powder into the extracting solution, and carrying out ultrasonic extraction to obtain a crude extracting solution; wherein, the ultrasonic extraction conditions are as follows: the extracting solution is acidified ethanol solution, the material-liquid ratio is 1:40), the extracting temperature is 80 ℃, the extracting time is 30min, the ultrasonic power is 200W, the ethanol concentration of the acidified ethanol solution is 80%, and the hydrochloric acid concentration is 1.00%.

3) Purifying and eluting the crude extract with DA201-C macroporous resin to obtain Prunus cerasifera pericarp extract; wherein the purification elution conditions are as follows: ethanol with the concentration of 80% is used as eluent, and the volume ratio of the crude extraction liquid to the macroporous resin column is 1: 20) the elution flow rate was 5mL/min, and 7 column volumes were eluted.

2) heating the Chinese medicinal powder and red wine to 70 deg.C, mixing at 100r/min, and stirring for 5 min;

3) adding Prunus cerasifera pericarp extract, Prunus cerasifera fruit extract and Prunus cerasifera seed extract, mixing, and fermenting at 37 deg.C for 2 days to obtain fermented product;

Example 3

55 parts of red wine, 12 parts of cherry plum peel extract, 15 parts of cherry fruit extract, 15 parts of cherry plum kernel extract, 12 parts of bighead atractylodes rhizome, 15 parts of white poria, 13 parts of bletilla striata, 6 parts of stiff silkworm, 12 parts of white paeony root, 8 parts of golden cypress, 6 parts of Chinese gall, 8 parts of fructus cnidii, 6 parts of semen coicis and 0.15 part of salicylic acid.

1) collecting cherry peel, pulping, and performing vacuum freeze drying and crushing pretreatment to obtain cherry peel powder; wherein, the vacuum freeze-drying conditions are as follows: the temperature is-70 ℃, the vacuum degree is 20Pa, and the drying time is 48 h.

2) Adding the cherry peel powder into the extracting solution, and performing ultrasonic extraction to obtain a crude extracting solution; wherein, the ultrasonic extraction conditions are as follows: the extract is acidified ethanol solution with material-liquid ratio of 1:20, extraction temperature of 50 deg.C, extraction time of 60min, ultrasonic power of 600W, ethanol concentration of acidified ethanol solution of 60%, and hydrochloric acid concentration of 0.05%.

3) Purifying and eluting the crude extract with DA201-C macroporous resin to obtain Prunus cerasifera pericarp extract. Wherein the conditions of the purification elution are as follows: ethanol with the concentration of 70-80% is used as eluent, and the volume ratio of the crude extract to the macroporous resin column is 1: (3-20), the elution flow rate is 1-5 mL/min, and 3-7 column volumes are eluted.

2) heating the Chinese medicinal powder and red wine to 60 deg.C, mixing at 60r/min, and stirring for 8 min;

3) adding Prunus cerasifera pericarp extract, Prunus cerasifera fruit extract, and Prunus cerasifera seed extract, mixing, and fermenting at 35 deg.C for 3 days to obtain fermented product;

Example 4

58 parts of red wine, 11 parts of cherry plum peel extract, 16 parts of cherry fruit extract, 18 parts of cherry plum kernel extract, 18 parts of bighead atractylodes rhizome, 12 parts of white poria, 13 parts of bletilla striata, 8 parts of stiff silkworm, 11 parts of white peony root, 9 parts of golden cypress, 6 parts of Chinese gall, 8 parts of fructus cnidii, 5 parts of semen coicis and 0.2 part of salicylic acid.

2) Adding the cherry peel powder into the extracting solution, and carrying out ultrasonic extraction to obtain a crude extracting solution; wherein, the ultrasonic extraction conditions are as follows: the extraction solution is acidified ethanol solution with a material-liquid ratio of 1:30, an extraction temperature of 70 ℃, an extraction time of 40min and an ultrasonic power of 500W, wherein the ethanol concentration of the acidified ethanol solution is 70%, and the hydrochloric acid concentration is 0.05%.

3) Purifying and eluting the crude extract with DA201-C macroporous resin to obtain Prunus cerasifera pericarp extract. Wherein the purification elution conditions are as follows: ethanol with the concentration of 70-80% is used as eluent, and the volume ratio of the crude extract to the macroporous resin column is 1: (3-20), the elution flow rate is 1-5 mL/min, and 3-7 column volumes are eluted.

2) heating the Chinese medicinal powder and red wine to 60 deg.C, mixing at 60r/min, and stirring for 6 min;

3) adding Prunus cerasifera pericarp extract, Prunus cerasifera fruit extract and Prunus cerasifera seed extract, mixing, and fermenting at 37 deg.C for 5 days to obtain fermented product;

Example 5

56 parts of red wine, 14 parts of cherry plum peel extract, 17 parts of cherry fruit extract, 16 parts of cherry plum kernel extract, 13 parts of bighead atractylodes rhizome, 14 parts of white poria, 13 parts of bletilla striata, 7 parts of stiff silkworm, 15 parts of white paeony root, 16 parts of golden cypress, 6 parts of Chinese gall, 7 parts of fructus cnidii, 10 parts of coix seed and 0.1 part of salicylic acid.

1) collecting cherry peel, pulping, and performing vacuum freeze drying and crushing pretreatment to obtain cherry peel powder; wherein the vacuum freeze-drying conditions are as follows: the temperature is-75 ℃, the vacuum degree is 15Pa, and the drying time is 40 h.

2) Adding the cherry peel powder into the extracting solution, and carrying out ultrasonic extraction to obtain a crude extracting solution; wherein, the ultrasonic extraction conditions are as follows: the extract is acidified ethanol solution with material-liquid ratio of 1:30, extraction temperature of 60 deg.C, extraction time of 100min, ultrasonic power of 500W, ethanol concentration of acidified ethanol solution of 50%, and hydrochloric acid concentration of 0.08%.

3) Purifying and eluting the crude extract with DA201-C macroporous resin to obtain Prunus cerasifera pericarp extract. Wherein the conditions of the purification elution are as follows: ethanol with the concentration of 70-80% is used as eluent, and the volume ratio of the crude extract to the macroporous resin column is 1: 15, elution flow 14mL/min, elution 5 column volumes.

2) heating the Chinese medicinal powder and red wine to 55 deg.C, mixing at 70r/min, and stirring for 6 min;

3) adding Prunus cerasifera pericarp extract, Prunus cerasifera fruit extract and Prunus cerasifera seed extract, mixing, and fermenting at 34 deg.C for 4 days to obtain fermented product;

Comparative example 1

Comparative example 1 differs from example 2 in that: comparative example 1 no cherry plum peel extract was added. The remaining amounts and preparation were the same as in example 2.

Comparative example 2

Comparative example 2 differs from example 2 in that: comparative example 2 no cherry plum kernel extract was added.

The remaining amounts and preparation were the same as in example 2.

Comparative example 3

Comparative example 3 differs from example 2 in that: comparative example 3 No Atractylodis rhizoma, Poria, rhizoma Bletillae, Bombyx Batryticatus, radix Paeoniae alba, and Coicis semen were added. The remaining amounts and preparation were the same as in example 2.

Comparative example 4

Comparative example 4 differs from example 2 in that: comparative example 4 no phellodendron parts, gallnut and cnidium fruit were added. The remaining amounts and preparation were the same as in example 2.

Comparative example 5

Comparative example 5 differs from example 2 in that: comparative example 5 no salicylic acid was added. The remaining amounts and preparation were the same as in example 2.

Comparative example 6

Comparative example 6 differs from example 2 in that: the preparation method of the cherry plum peel extract of comparative example 6 was: collecting cherry peel, pulping, vacuum freeze drying, and pulverizing to obtain cherry peel powder. The remaining amounts and preparation were the same as in example 2.

Comparative example 7

Comparative example 7 differs from example 2 in that: comparative example 7 No red wine was added, and distilled water was used instead. The remaining amounts and preparation were the same as in example 2.

Performance test

First, moisture retention test

The test method comprises the following steps: 5.0g of each of the compositions of examples 1 to 5 and comparative examples 1 to 6 was weighed, placed in weighing bottles, and placed in an environment of 43% and 81% relative humidity at room temperature (24 to 30 ℃) for 24 hours, and the moisture retention rate was calculated according to formula (1).

In the formula, m₀G, the mass of a sample to be detected; m is_nMass of sample, g, after 24 hours of standing.

The moisturizing rate data for each group is shown in table 1.

TABLE 1 moisture retention data for each group

As can be seen from Table 1, the moisturizing rates of examples 1 to 5 are all superior to those of comparative examples 1 to 7, and the combination of red wine, cherry plum peel extract, cherry fruit extract, cherry plum kernel extract, white atractylodes rhizome, white poria, bletilla striata, silkworm larva, white peony root, phellodendron bark, Chinese gall, fructus cnidii, coix seed and salicylic acid can achieve the optimal moisturizing effect.

Second, test of whitening Performance

The preparation method of the solution to be detected comprises the following steps: weighing 1.0g of each sample, uniformly dispersing the samples by using distilled water, transferring the samples into a 100mL volumetric flask for constant volume, and respectively diluting the samples to be detected until the concentration of the samples is 5.0 mg/mL.

The method for measuring the tyrosinase inhibition rate comprises the following steps: respectively preparing a Phosphate Buffer Solution (PBS) with the pH value of 6.83, a tyrosinase solution with the mass concentration of 0.07mg/mL and an L-tyrosinase solution with the mass concentration of 1.0 mg/mL. Accurately preparing a solution to be detected 1: 2.0mL of PBS +0.5mL of tyrosinase solution +0.5mL of L-tyrosine solution; and (3) liquid to be detected 2: 2.5mL PBS +0.5mL tyrosinase solution; solution to be tested 3: 0.5mL of a solution to be tested, 1.5mL of PBS, 0.5mL of tyrosinase solution and 0.5mL of tyrosine solution; and (4) a to-be-detected liquid: 0.5mL of the solution to be tested +2.0mL of LPBS +0.5mL of the tyrosinase solution. Placing in 37 deg.C water bath, keeping the temperature for 30min, immediately measuring absorbance at 475nm, and recording the absorbance as A₁、A₂、A₃、A₄. Tyrosinase inhibition was calculated according to formula (2). The data are shown in table 2.

I＝[(A₁-A₂)-(A₃-A₄)]/(A₁-A₂)×100％ (2)

Third, testing the oxidation resistance

Method for measuring DPPH-radical inhibition ratio: adding 3mL of the solution to be detected and 3mL of 0.2mmol/L DPPH-ethanol solution into the same test tube with a plug, shaking, standing in the dark at room temperature for 30min, and measuring the absorbance A at the wavelength of 517nm_i1The absorbance A of the mixture of the extractant and 3mL each of the DPPH and ethanol solutions was measured by the same method₀₁And absorbance A of the mixture of the solution to be detected and 3mL of absolute ethyl alcohol_j1The inhibition ratio P is calculated according to the formula (3). The data are shown in table 2.

P＝[1-(A_i1-A_j1)/A₀₁]×100％。

Table 2 inhibition of tyrosinase and free radical by the compositions of each group

Group of	I/％	P/％
			Example 1	46.3	53.8
Example 2	51.8	57.2
			Example 3	48.5	54.9
Example 4	49.6	56.1
			Example 5	47.3	54.2
Comparative example 1	38.6	43.6
			Comparative example 2	39.2	45.2
Comparative example 3	42.7	49.7
			Comparative example 4	43.1	50.6
Comparative example 5	41.2	48.2
			Comparative example 6	40.4	47.7
Comparative example 7	39.8	46.8

As can be seen from table 2, in examples 1 to 5, the highest inhibitory activity against tyrosinase and DPPH free radical was demonstrated in example 2, indicating that both whitening and antioxidant effects were the best. The parameters influencing the whitening and antioxidant effects can be sequentially listed according to the size of the whitening and antioxidant effect data as follows: (influence degree is from large to small) comparative example 1 no cherry plum peel extract was added, comparative example 2 no cherry plum kernel extract was added, comparative example 7 no red wine was added, comparative example 6 cherry plum peel extract was merely pulverized to prepare powder, comparative example 5 no salicylic acid was added, comparative example 3 no (atractylodes macrocephala koidz, poria alba, bletilla striata, stiff silkworm, white peony root and coix seed) and comparative example 4 no phellodendron amurense, gallnut and cnidium fruit were added. The composition has obvious whitening effect and antioxidant effect, no toxic or side effect and good effect.

The above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention is not limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are within the protection scope of the present invention.

Claims

1. The whitening and moisturizing traditional Chinese medicine composition is characterized by comprising the following raw materials:

2. The whitening and moisturizing traditional Chinese medicine composition of claim 1, which comprises the following raw materials in parts by mass:

50-60 parts of red wine, 10-15 parts of cherry plum peel extract, 10-20 parts of cherry fruit extract, 10-20 parts of cherry plum kernel extract, 10-20 parts of bighead atractylodes rhizome, 10-20 parts of white poria, 10-15 parts of bletilla striata, 5-10 parts of stiff silkworm, 10-20 parts of white paeony root, 5-10 parts of golden cypress, 5-10 parts of Chinese gall, 5-10 parts of fructus cnidii, 5-10 parts of semen coicis and 0.1-0.2 part of salicylic acid.

3. The whitening and moisturizing traditional Chinese medicine composition as claimed in claim 1, wherein the preparation method of the cherry plum peel extract comprises:

2) adding ethanol into cherry peel powder, and performing ultrasonic extraction to obtain a crude extract;

4. The whitening and moisturizing traditional Chinese medicine composition of claim 3, wherein the vacuum freeze-drying conditions are as follows: the temperature is-78 to-70 ℃, the vacuum degree is 15 to 20Pa, and the drying time is 40 to 48 hours.

5. The whitening and moisturizing traditional Chinese medicine composition of claim 3, wherein the conditions of purification and elution are as follows: ethanol with the concentration of 70-80% is used as eluent, and the volume ratio of the crude extract to the macroporous resin column is 1: (3-20), the elution flow rate is 1-5 mL/min, and 3-7 column volumes are eluted.

6. The preparation method of the whitening and moisturizing traditional Chinese medicine composition as claimed in any one of claims 1 to 5, which is characterized by comprising the following steps:

2) mixing the Chinese medicinal powder with red wine, and stirring;

7. The preparation method of the whitening and moisturizing traditional Chinese medicine composition as claimed in claim 6, wherein in the step 2), the mixing speed is 50-100 r/min, and the stirring time is 5-10 min.

8. The preparation method of the whitening and moisturizing traditional Chinese medicine composition as claimed in claim 6, wherein in the step 2), the mixing is performed at 50-70 ℃.

9. The preparation method of the whitening and moisturizing traditional Chinese medicine composition as claimed in claim 6, wherein in the step 3), the fermentation is carried out at 32-37 ℃ for 2-5 days.

10. The application of the whitening and moisturizing traditional Chinese medicine composition as defined in any one of claims 1 to 5, wherein the whitening and moisturizing traditional Chinese medicine composition is applied to masks, essences, toning water and creams.