CN114618849B

CN114618849B - Cleaning device, cleaning method and sample analysis device for magnetic separation reaction cup

Info

Publication number: CN114618849B
Application number: CN202011459405.3A
Authority: CN
Inventors: 王能河; 刘文斌
Original assignee: Shenzhen Dymind Biotechnology Co Ltd
Current assignee: Shenzhen Dymind Biotechnology Co Ltd
Priority date: 2020-12-11
Filing date: 2020-12-11
Publication date: 2023-07-18
Anticipated expiration: 2040-12-11
Also published as: CN114618849A

Abstract

The application discloses a cleaning device, a cleaning method and a sample analysis device for a magnetic separation reaction cup. The cleaning device of the magnetic separation reaction cup comprises: the device comprises a magnetic separation disc and a plurality of operating mechanisms, wherein a plurality of stations are arranged on the magnetic separation disc and used for placing reaction cups; the operating mechanisms are arranged in one-to-one correspondence with the stations; the magnetic separation disc rotates at least two stations each time, so that when the reaction cup moves to a corresponding operation mechanism according to the cleaning time sequence of the reaction cup on each station, the corresponding operation mechanism is controlled to operate the reaction cup, the number of the rotating working sites each time is smaller than the total number of the working sites, and the integral multiple of the number of the rotating working sites each time is unequal to the total number of the working sites. Through the mode, the device can be simplified, and the device can meet cleaning requirements of various stages.

Description

Cleaning device, cleaning method and sample analysis device for magnetic separation reaction cup

Technical Field

The present invention relates to the field of medical detection technology, and in particular, to a cleaning device, a cleaning method, and a sample analyzer for a magnetic separation cuvette.

Background

The chemical immunoassay technology is mainly a means for detecting by utilizing the specific reaction of an antigen and an antibody, and is often used for detecting trace substances such as proteins and hormones because detection signals can be amplified and displayed by isotopes, enzymes, chemiluminescent substances and the like.

Magnetic bead separation is an important step in chemical immunoassays, and magnetic beads are magnetic microspheres coated with specific biomolecules that can specifically bind to a target substance containing the same to form a new complex, which can be retained by a magnetic field and separated from other components. The method is mainly applied to protein purification, cell separation and the like. The core of the magnetic bead technology is that a proper external magnetic field is adopted to magnetize and adsorb the magnetic beads on the inner wall of the reaction cup, when the external magnetic field is removed, the magnetism of the magnetic beads disappears, the magnetic beads are dispersed in the solution again, and the magnetic beads are scattered and gathered continuously by utilizing the characteristic, so that the magnetic beads are cleaned. The existing magnetic separation cleaning equipment is often provided with a large number of stations, and the magnetic beads are gathered and cleaned one by one.

Disclosure of Invention

The application mainly provides a cleaning device, a cleaning method and a sample analysis device for a magnetic separation reaction cup, which can solve the problems of complex equipment, single cleaning process and time waste in the prior art.

To solve the above technical problem, a first aspect of the present application provides a cleaning device for a magnetic separation reaction cup, the device includes: the magnetic separation disc is provided with a plurality of stations for placing reaction cups; the operating mechanisms are arranged in one-to-one correspondence with the stations; the magnetic separation disc rotates at least two stations each time, so that when the reaction cup moves to a corresponding operation mechanism according to the cleaning time sequence of the reaction cup on each station, the corresponding operation mechanism is controlled to operate the reaction cup, the number of the rotating working sites each time is smaller than the total number of the working sites, and the integral multiple of the number of the rotating working sites each time is unequal to the total number of the working sites.

To solve the above technical problem, a second aspect of the present application provides a method for cleaning a magnetic separation reaction cup, where the method is applied to the cleaning device for a magnetic separation reaction cup provided in the first aspect, and the method includes: the cup grabbing mechanism is controlled to place the reaction cup to be cleaned at a corresponding station of the magnetic separation disc; controlling the magnetic separation disc to rotate at least two stations each time; and according to the cleaning time sequence of the reaction cup, when the reaction cup moves to an operating mechanism corresponding to the current cleaning time sequence, controlling the operating mechanism to operate the reaction cup.

To solve the above technical problem, a third aspect of the present application provides a sample analysis device, including: the magnetic separation disc is provided with a plurality of stations for placing reaction cups; the operating mechanisms are arranged in one-to-one correspondence with the stations; a memory for storing program data; and a controller connected to the memory, the magnetic separation disk, and the plurality of operating mechanisms for executing the program data to implement the method for cleaning a magnetic separation cuvette as provided in the second aspect.

The beneficial effects of this application are: the cleaning device is provided with a magnetic separation disc with a plurality of stations, is used for placing the reaction cup, and is provided with an operating mechanism corresponding to each station, the magnetic separation disc rotates at least two stations each time, so that when the reaction cup moves to the corresponding operating mechanism according to the cleaning time sequence of the reaction cup on each station, the corresponding operating mechanism is controlled to operate the reaction cup, wherein the number of the rotating working bits each time is smaller than the total number of the working bits, and the integral multiple of the number of the rotating working bits each time is unequal to the total number of the working bits. Therefore, the reaction cup can reach the corresponding station by controlling the rotation of the magnetic separation disc, the station quantity of the magnetic separation disc can be reduced, and the device is simplified by controlling the number of rotation turns of the magnetic separation disc and controlling the station number of the reaction cup; further, through the time sequence of the station that the reaction cup arrived and the cleaning step, the operation time sequence of each operating mechanism that the control station corresponds to the cooperation is accomplished magnetism separation cleaning operation, adapts to the washing demand of multiple different stages.

Drawings

FIG. 1 is a schematic illustration of the structure of an embodiment of a magnetic separation disk of the present application;

FIG. 2 is a schematic illustration of another embodiment of a magnetic separation disk of the present application;

FIG. 3 is a schematic diagram showing the distribution of an operating mechanism of an embodiment of a cleaning device for magnetic separation reaction cups;

FIG. 4 is a schematic view showing the distribution of operating mechanisms of another embodiment of the cleaning device for magnetic separation reaction cups of the present application;

FIG. 5 is a schematic view of an embodiment of a cleaning apparatus for magnetic separation reaction cups of the present application;

FIG. 6 is a schematic block diagram illustrating one embodiment of a method for cleaning a magnetic separation cuvette in accordance with the present application;

FIG. 7 is a schematic diagram of a station flowchart of one embodiment of a first-stage cleaning of a cuvette using the apparatus of FIG. 5;

FIG. 8 is a schematic diagram of a station flowchart of one embodiment of a second order cleaning of a cuvette using the apparatus of FIG. 5;

FIG. 9 is a schematic diagram of a station flowchart showing one embodiment of a third-order cleaning of a cuvette using the apparatus of FIG. 5;

FIG. 10 is a schematic view of a magnetic separation mechanism according to an embodiment of the present invention;

FIG. 11 is a schematic diagram of a circuit connection of an embodiment of the sample analyzer of the present application;

fig. 12 is a schematic block diagram of a circuit configuration of an embodiment of a computer-readable storage medium of the present application.

Detailed Description

The following description of the technical solutions in the embodiments of the present application will be made clearly and completely with reference to the accompanying drawings in the embodiments of the present application, and it is apparent that the described embodiments are only some embodiments of the present application, not all embodiments. All other embodiments, which can be made by one of ordinary skill in the art without undue burden from the present disclosure, are within the scope of the present disclosure.

The terms "first," "second," and the like in this application are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of features shown. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. Furthermore, the terms "comprise" and "have," as well as any variations thereof, are intended to cover a non-exclusive inclusion. For example, a process, method, system, article, or apparatus that comprises a list of steps or elements is not limited to only those listed steps or elements but may include other steps or elements not listed or inherent to such process, method, article, or apparatus.

Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment may be included in at least one embodiment of the present application. The appearances of such phrases in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. Those of skill in the art will explicitly and implicitly understand that the embodiments described herein may be combined with other embodiments.

The application first provides a belt cleaning device of magnetic separation reaction cup, and this device is used for wasing the magnetic bead that is through incubating, gets rid of free interfering substance therein. Referring to fig. 1, fig. 1 is a schematic diagram illustrating a structure of an embodiment of a magnetic separation disc, and the magnetic separation disc 10 is provided with a plurality of stations 101 for placing reaction cups, and the stations are arranged in one-to-one correspondence with the operating mechanisms so as to operate the reaction cups placed at the corresponding stations 101 through the operating mechanisms. Specifically, the operating mechanism is used for performing operations such as liquid injection, liquid discharge, magnetic attraction and the like on the reaction cup, so as to clean the magnetic beads.

Wherein, the magnetic separation disc 10 rotates at least two stations each time, so as to control the corresponding operating mechanism to operate the reaction cup when the reaction cup moves to the corresponding operating mechanism according to the cleaning time sequence of the reaction cup on each station, wherein, the number of the rotating stations is smaller than the total number of the stations each time, and the integral multiple of the number of the rotating stations is unequal to the total number of the stations each time.

Alternatively, the magnetic separation disc may be rotated by two stations, three stations, four stations, or the like. Thus, after the reaction cup at each station is rotated once through the magnetic separation disc, the reaction cup at each station is moved to a position separated from the original position by at least two stations, and then the operation of each operation mechanism is controlled according to the time sequence of the reaction cup reaching each operation mechanism and the time sequence of each step of the reaction cup cleaning flow, so that when the reaction cup reaches the corresponding position, the operation mechanism is carried out according to the correct cleaning time sequence, and when the reaction cup rotates along with the magnetic separation disc, the operation mechanisms are matched to complete the cleaning operation of the reaction cup, such as the injection of cleaning liquid, the discharge of the cleaning liquid, the magnetic adsorption and the like, to the reaction cup.

For example, when the rotation amplitude is two stations, the total station number can be 5, 7, 9 and the like, and all operating mechanisms can be reached when the magnetic separation disc rotates for two weeks; when the rotation amplitude is three stations, the total station number can be 7, 8, 10 and the like, all operating mechanisms can be reached when the magnetic separation disc rotates for three weeks, and when the rotation amplitude is four stations, the total station number can be 9, 11, 13 and the like. The magnetic separation disc can be controlled to only reach the stations corresponding to part of the operating mechanisms when rotating the first circle, and reach the stations corresponding to other operating mechanisms when rotating the second circle or the third circle. The number of times of the first-order cleaning is small, the period is short, the number of times of the rotation of the magnetic separation disc is small, the number of times of the second-order cleaning and the third-order cleaning is large, the period is long, and the number of times of the rotation of the magnetic separation disc is large. Therefore, when the multi-stage cleaning is needed, the rotation times of the magnetic separation disc are controlled to be increased, and the multi-stage cleaning can be realized by controlling the operation mechanisms to operate cooperatively, so that a large number of stations are not needed to be arranged on the magnetic separation disc, the occupied space of the cleaning device can be effectively saved, and the miniaturized design of the device is realized.

Furthermore, in order to allow the reaction cups located at each station to reach all operating mechanisms after a limited number of rotations of the magnetic separation discs, the total number of stations and the number of stations per rotation cannot be the same even number.

Wherein, among the plurality of operating means, including magnetic separation mechanism, flowing back mechanism and annotate liquid mechanism. The liquid discharge mechanism is used for discharging the liquid in the reaction cup, and the liquid injection mechanism is used for injecting cleaning liquid or detecting a substrate in the reaction cup after the liquid discharge.

Further, the liquid injection mechanism can inject a cleaning liquid or a detection substrate into the reaction cup by using a short needle, and the cleaning liquid is used for cleaning the magnetic beads. The detection substrate can be a cleaning solution for cleaning the magnetic beads, and can also be a luminescent substrate (enzymatic chemiluminescence) or an oxidant (direct chemiluminescence). The liquid draining mechanism can extend into the reaction cup by using the long needle so as to drain the cleaning liquid in the reaction cup through suction.

The reaction cup is required to be moved to a detection area after the detection substrate is injected into the reaction cup for detection; if the reaction cup does not need to be detected, the detection substrate does not need to be injected into the reaction cup.

Referring to FIG. 2, FIG. 2 is a schematic diagram of another embodiment of a magnetic separation disk. The dashed line 200 in the figure is a transfer path of the reaction cup, two sides of the transfer path exist, the magnetic separation mechanism can be arranged on one side of the transfer path 200 and is opposite to the bottom of the reaction cup, when the reaction cup is transferred to the magnetic separation mechanism, the magnetic beads in the reaction cup are adsorbed, and the magnetic beads in the reaction cup move to one side close to the magnetic piece and gather under the adsorption of the magnetic piece. For example, the magnetic separation mechanisms 201 and 202 are arranged in two different ways, when the reaction cup moves to the position of the magnetic separation mechanism 201, the magnetic beads are gathered in the direction approaching the magnetic separation mechanism 201, and when the reaction cup moves to the position of the magnetic separation mechanism 202, the magnetic beads are gathered in the direction approaching the magnetic separation mechanism 202.

Referring to fig. 3, fig. 3 is a schematic distribution diagram of an operating mechanism of an embodiment of a cleaning device for a magnetic separation reaction cup of the present application. This embodiment is described with a total number of 5 stations for each rotation of the magnetic separator disk.

The magnetic separation disc comprises 5 stations, each station corresponds to an operating mechanism, and the operating mechanisms are respectively as follows: the first actuator 301, the second actuator 302, the third actuator 303, the fourth actuator 304 and the fifth actuator 305, in this embodiment the magnetic separation discs are rotated two stations at a time in a counter clockwise direction. Taking the cleaning process of the reaction cup with the initial position at the corresponding position of the first operating mechanism 301 as an example, the magnetic separation plate rotates for one circle, and the reaction cups are sequentially positioned: the first operating mechanism 301, the third operating mechanism 303 and the fifth operating mechanism 305, the magnetic separation disc rotates once again, and the reaction cups are positioned in sequence: a second operating mechanism 302, a fourth operating mechanism 304, and a first operating mechanism 301. That is, the magnetic separator rotates once, passes through part of the operating mechanisms (the first operating mechanism 301, the third operating mechanism 303, and the fifth operating mechanism 305), rotates once again, passes through the other part of the operating mechanisms (the second operating mechanism 302, and the fourth operating mechanism 304), rotates two times in succession, and then undergoes all the operating mechanisms.

According to the transfer path of the reaction cup, namely, a first operation mechanism 301, a third operation mechanism 303, a fifth operation mechanism 305, a second operation mechanism 302, a fourth operation mechanism 304, a first operation mechanism 301, and a cleaning step of the reaction cup, one of the types of each operation mechanism such as a magnetic separation mechanism, a liquid injection mechanism, a liquid discharge mechanism, and the like can be set, for example, the third operation mechanism 303 is a liquid injection mechanism, the second operation mechanism 302 is a liquid discharge mechanism, the other operation mechanisms are magnetic separation mechanisms, and the like, when the position of the reaction cup is in the third operation mechanism 303, the third operation mechanism 303 is controlled to inject cleaning liquid into the reaction cup, when the position of the reaction cup is in the second operation mechanism 302, the second operation mechanism 302 is controlled to discharge cleaning waste liquid in the reaction cup, and at the first operation mechanism 301, the fourth operation mechanism 304, and the fifth operation mechanism 305, aggregation and scattering of magnetic beads are realized by utilizing a magnetic adsorption effect, so that the magnetic beads are fully contacted with the cleaning liquid, and the cleaning is fully completed.

In this embodiment, if more steps of cleaning are to be implemented, the magnetic separation disc may be controlled to rotate for multiple times, and each operating mechanism is controlled to cooperate, so as to implement multi-step cleaning, for example, in this embodiment, the magnetic separation disc may be controlled to rotate around, that is, rotate for 10 times, where the transfer path of the reaction cup is: the first operating mechanism 301-the third operating mechanism 303-the fifth operating mechanism 305-the second operating mechanism 302-the fourth operating mechanism 304-the first operating mechanism 301, and when the position of the reaction cup is in the third operating mechanism 303, the third operating mechanism 303 is controlled to inject the cleaning liquid into the reaction cup, and when the position of the reaction cup is in the second operating mechanism 302, the second operating mechanism 302 is controlled to discharge the cleaning waste liquid in the reaction cup, and when the reaction cup is in other operating mechanisms, the magnetic adsorption is utilized to repeatedly scatter and gather the magnetic beads in the reaction cup. To finish the cleaning of more steps, the magnetic separation disc is further controlled to rotate 15 times, 20 times and the like, and the operation of each operating mechanism in the cleaning process of more steps is similar to that of the first-order cleaning and the second-order cleaning, and is not repeated.

The arrangement of the types of the operating mechanisms in this embodiment is only schematically illustrated, and other arrangements of the types and the sequence of the operating mechanisms are possible according to the number of aggregation times required for each cleaning stage and the cleaning force and sequence, for example, the third operating mechanism 303 may be configured as a liquid filling mechanism, and the fourth operating mechanism 304 may be configured as a liquid draining mechanism.

In an embodiment, the plurality of operating mechanisms further comprise a cup grabbing mechanism and a liquid dispensing mechanism, wherein the cup grabbing mechanism is used for placing the reaction cup on the station or removing the reaction cup from the station, and the liquid dispensing mechanism is used for adding the cleaning liquid to the liquid in the reaction cup to a preset volume.

Specifically, because the detected items are different, the used sample amount and the reagent addition total amount are different, the liquid level in the reaction cup is inconsistent when the reaction cup is placed in the magnetic separation disc, the magnetic piece setting height of the magnetic separation mechanism is fixed, the magnetic separation mechanism has poor adsorption effect on the magnetic beads, and the liquid level in the reaction cup is configured to a certain height by the liquid preparation mechanism, so that the magnetic beads can be uniformly distributed on the inner wall of the reaction cup when the magnetic separation mechanism performs magnetic adsorption aggregation on the magnetic beads, and the cleaning effect of the magnetic beads is improved.

The liquid distribution mechanism is further used for judging the liquid quantity in the reaction cup located at the corresponding position to determine whether the preset capacity is reached, if the preset capacity is reached, the cleaning liquid is not added to the reaction cup, and if the preset capacity is not reached, the cleaning liquid is added to the reaction cup to reach the preset capacity. Specifically, the liquid dispensing mechanism is equipped with a liquid volume detecting instrument to detect whether the amount of liquid in the cuvette reaches a preset volume.

In one embodiment, a magnetic separation mechanism is arranged between two adjacent liquid draining mechanisms, and a magnetic separation mechanism is arranged between two adjacent liquid injecting mechanisms, wherein the number of working bits per rotation is 3.

The magnetic separation mechanism is arranged near the bottom of the reaction cup, and the cup grabbing mechanism, the liquid distribution mechanism, the liquid injection mechanism and the liquid discharge mechanism are arranged near the cup mouth of the reaction cup without mutual influence, so that the magnetic separation mechanism, the cup grabbing mechanism, the liquid distribution mechanism, the liquid injection mechanism and the liquid discharge mechanism can be arranged corresponding to one station at the same time.

For example, the magnetic separation mechanism can be matched with the corresponding station of the cup grabbing mechanism, so that the reaction cup can be transferred to the magnetic separation disc by the cup grabbing mechanism, or the reaction cup can be magnetically adsorbed when being transferred to the corresponding station of the cup grabbing mechanism along with the rotation of the magnetic separation disc; the magnetic separation mechanism can be matched with the corresponding station of the liquid preparation mechanism, so that the magnetic adsorption operation and the liquid preparation operation can be simultaneously carried out when the reaction cup is transported to the corresponding station of the liquid preparation mechanism; the corresponding stations of the liquid injection mechanism can be matched with the magnetic separation mechanism, so that when the reaction cup is transported to the corresponding stations of the liquid injection mechanism, the magnetic adsorption operation and the liquid injection operation can be simultaneously carried out; the corresponding station of the liquid discharging mechanism can be matched with the magnetic separation mechanism, so that the magnetic adsorption operation and the liquid discharging operation can be simultaneously carried out when the reaction cup is transported to the corresponding station of the liquid discharging mechanism.

In a specific embodiment, the magnetic separation mechanism can be matched with all operating mechanisms except the magnetic separation mechanism, so that the magnetic beads can be magnetically adsorbed when the reaction cup is transported to any station, and the magnetic beads can be thoroughly cleaned.

Specifically, referring to fig. 4, in the multiple operation mechanisms, two liquid injection mechanisms 402 and 404 and two liquid discharge mechanisms 405 and 407 are included, a magnetic separation mechanism 403 is disposed between the two liquid injection mechanisms 402 and 404 at intervals, and a magnetic separation mechanism 406 is disposed between the two liquid discharge mechanisms 405 and 407 at intervals.

Thus, when the magnetic separation disk rotates 3 stations in either a counterclockwise or clockwise direction, the transfer path of the cuvette that transfers from 401 to 404 for draining is: 401-402-403-404, the middle of which is subjected to magnetic bead scattering and aggregation through a magnetic separation mechanism 403; the transfer path of the reaction cup transferred from the 405 position to the 402 position for draining is as follows: 405-404-403-402, the middle of which is subjected to magnetic bead scattering and aggregation through a magnetic separation mechanism 403; the transfer path of the reaction cup transferred from the 408 position to the 405 position for filling is as follows: 408-407-406-405, the middle of which is subjected to magnetic bead scattering and aggregation through the magnetic separation mechanism 406; the transfer path of the reaction cup transferred from the 404 position to the 407 position for filling is as follows: 404-405-406-407, the magnetic beads are scattered and collected via the magnetic separation mechanism 406. That is, each reaction cup to be filled with liquid or discharged with liquid can be adsorbed, gathered and scattered by the magnetic separation mechanism, so that the cleaning effect of the magnetic beads is ensured.

Referring to fig. 5, fig. 5 is a schematic view of an embodiment of a cleaning apparatus for a magnetic separation cuvette according to the present application.

The number of the working positions of each rotation of the magnetic separation disc is 3, the total number of the working positions is 11, each working position is correspondingly provided with an operating mechanism, and the operating mechanisms sequentially comprise: a cup grabbing mechanism 501, a liquid preparation mechanism 502, a first magnetic separation mechanism 503, a second magnetic separation mechanism 504, a third magnetic separation mechanism 505, a first liquid discharge mechanism 506, a fourth magnetic separation mechanism 507, a second liquid discharge mechanism 508, a first liquid injection mechanism 509, a fifth magnetic separation mechanism 510, and a second liquid injection mechanism 511.

The second liquid injection mechanism 511 and the first liquid injection mechanism 509 of the present embodiment are configured to be capable of injecting a cleaning liquid into a cuvette or detecting a substrate.

The magnetic separation disk of this embodiment is rotated once every 5 to 20s, for example, once every 7s, 10s, 12.5s, and 14 s.

Under the device of this embodiment, the reaction cup can be located in proper order under the rotation of magnetic separation dish: a cup grabbing mechanism 501, a second magnetic separation mechanism 504, a fourth magnetic separation mechanism 507, a fifth magnetic separation mechanism 510, a liquid preparation mechanism 502, a third magnetic separation mechanism 505, a second liquid discharge mechanism 508, a second liquid injection mechanism 511, a first magnetic separation mechanism 503, a first liquid discharge mechanism 506, a first liquid injection mechanism 509, and a cup grabbing mechanism 501.

The general flow of the device for cleaning the reaction cup is as follows: the reaction cup is added to the station of the magnetic separation disc by utilizing the cup grabbing mechanism 501, the reaction cup is sequentially driven to the second magnetic separation mechanism 504, the fourth magnetic separation mechanism 507 and the fifth magnetic separation mechanism 510 along with the rotation of the magnetic separation disc, the reaction cup is driven to the liquid distribution mechanism 502 after the magnetic beads are adsorbed, gathered and scattered, the liquid distribution mechanism 502 adds cleaning liquid to the reaction cup according to the requirement, the reaction cup is driven to the third magnetic separation mechanism 505 along with the rotation of the magnetic separation disc, the magnetic beads are gathered again, the reaction cup is driven to the second liquid draining mechanism 508 along with the rotation of the magnetic separation disc, the second liquid draining mechanism 508 discharges liquid in the reaction cup, the reaction cup is driven to the second liquid injecting mechanism 511, the second liquid injecting mechanism 511 injects the cleaning liquid into the reaction cup, the reaction cup is driven to the first magnetic separation mechanism 503, the magnetic beads are gathered again, the reaction cup is driven to the first liquid draining mechanism 506, the liquid in the reaction cup is driven to the first liquid injecting mechanism 509, if the reaction cup is required to be detected, the first liquid injecting mechanism 509 is controlled to be detected, otherwise, the reaction cup is not injected to the substrate grabbing mechanism 501, and the reaction cup is not driven to be grabbed. Thus, the first-order cleaning is completed.

It is not difficult to find that the apparatus of this embodiment can make the reaction cup discharge the waste liquid to the second liquid discharge mechanism 508 after the reaction cup is dispensed by the liquid dispensing mechanism 502, the magnetic beads are gathered and scattered in the middle via the third magnetic separation mechanism 505, and the reaction cup discharge the waste liquid to the first liquid discharge mechanism 506 after the second liquid injection mechanism 511 injects the cleaning liquid, the magnetic beads are gathered and scattered in the middle via the first magnetic separation mechanism 503. That is, the magnetic beads are collected and dispersed by the magnetic adsorption mechanism between the pouring and discharging of the reaction cup, and the reaction cup is transferred from the liquid dispensing mechanism 502 to the second liquid discharging mechanism 508, and is transferred from the second pouring mechanism 511 to the first liquid discharging mechanism 506 via the first magnetic separating mechanism 503, the second magnetic separating mechanism 504 and the fourth magnetic separating mechanism 507, and is also sufficiently dispersed by the plurality of magnetic separating mechanisms during the cleaning, and the cleaning effect is good.

If the multi-stage cleaning is to be performed, the first liquid injection mechanism 509 may be controlled to inject the cleaning liquid into the reaction cup when the reaction cup is positioned in the first liquid injection mechanism 509, and the reaction cup may be continuously cleaned in more stages along with the rotation of the magnetic separation plate without taking out the reaction cup when the reaction cup is positioned in the cup grasping mechanism 501, and the cleaning process in more stages may be completed by controlling the respective operation mechanisms. Specifically, as the magnetic separation disk continues to rotate in the course of 3 stations per rotation, the reaction cups may again be positioned in sequence: the second magnetic separation mechanism 504, the fourth magnetic separation mechanism 507, the fifth magnetic separation mechanism 510, the liquid preparation mechanism 502, the third magnetic separation mechanism 505, the second liquid discharge mechanism 508, the second liquid injection mechanism 511, the first magnetic separation mechanism 503, the first liquid discharge mechanism 506, the first liquid injection mechanism 509, and the cup grabbing mechanism 501. When the reaction cup is positioned again in the first liquid discharging mechanism 506, the liquid in the reaction cup may be discharged, and when the reaction cup is positioned again in the first liquid injecting mechanism 509, it may be determined whether or not to inject the detection substrate into the reaction cup, if the reaction cup is to be detected next, the detection substrate is injected into the reaction cup, otherwise, the detection substrate is not injected into the reaction cup, and when the reaction cup is positioned again in the cup grasping mechanism 501, the reaction cup is taken out, and when the reaction cup is positioned again in the liquid dispensing mechanism 502, the second liquid discharging mechanism 508, and the second liquid injecting mechanism 511, no operation is performed. Alternatively, when the cuvette is again positioned in the second liquid discharging mechanism 508, the liquid in the cuvette may be discharged, and when the cuvette is again positioned in the second liquid charging mechanism 511, it may be determined whether or not to charge the detection substrate into the cuvette, and if the cuvette is to be detected next, the detection substrate is charged into the cuvette, otherwise, the detection substrate is not charged into the cuvette, and when the cuvette is again positioned in the cuvette grasping mechanism 501, the cuvette may be taken out, and when the cuvette is again positioned in the first liquid discharging mechanism 506 and the first liquid charging mechanism 509, no operation may be performed. Thus, the second-order cleaning is completed. The third-stage cleaning may be performed by discharging the liquid in the cuvette when the cuvette is again positioned in the second liquid discharging mechanism 508, by injecting the cleaning liquid into the cuvette when the cuvette is again positioned in the second liquid injecting mechanism 511, by discharging the liquid in the cuvette when the cuvette is again positioned in the first liquid discharging mechanism 506, by determining whether to inject the detection substrate into the cuvette when the cuvette is again positioned in the first liquid injecting mechanism 509, and by injecting the detection substrate into the cuvette if the cuvette is to be detected, or by not injecting the detection substrate into the cuvette and by taking out the cuvette when the cuvette is again positioned in the cuvette grabbing mechanism 501.

If more steps such as four-step cleaning, five-step cleaning and the like are required to be performed, each operating mechanism can be matched according to the control process of the two-step cleaning and the three-step cleaning so as to complete multi-step cleaning. And will not be described in detail herein.

Wherein the second liquid injection mechanism 511 and the first liquid injection mechanism 509 perform periodic configuration of injecting cleaning liquid or detecting substrate according to the cleaning cycle and the cleaning stage, so as to adapt to the requirement of multi-stage cleaning of two or more stages.

According to the embodiment, the rotating working positions of the magnetic separation disc, the total working positions, the arrangement mode of the operating mechanism and the operation time sequence of the operating mechanism are matched and adaptively set, so that the first-order to multi-order cleaning requirement can be met under the condition of setting fewer operating mechanisms and working positions, the occupied space of the device is effectively reduced, the miniaturized design of the device is realized, and further, when the first-order or second-order cleaning is carried out for fewer times, the reaction cup does not need to be subjected to a plurality of cleaning mechanisms in the cleaning stages, and the cleaning time is greatly saved.

Referring to fig. 6, fig. 6 is a schematic flow chart of an embodiment of a method for cleaning a magnetic separation cuvette according to the present application, which is applied to the cleaning apparatus according to the above embodiments of the present application. Specifically, the method of the present embodiment is a method for controlling each operating mechanism and magnetic separation discs of the cleaning apparatus according to each of the above embodiments of the present application, the method including:

S10: the cup grabbing mechanism is controlled to place the reaction cup to be cleaned at the corresponding station of the magnetic separation disc.

After incubation is completed, the cup grabbing mechanism is controlled to take out the reaction cup to be cleaned from the incubation plate and other areas, and the reaction cup is placed at a station of the magnetic separation plate corresponding to the cup grabbing mechanism, so that the reaction cup is driven to each operating mechanism when the magnetic separation plate rotates, and cleaning of the reaction cup is completed.

S20: the magnetic separation disk is controlled to rotate at least two stations at a time.

The magnetic separation discs are controlled to rotate and at least two stations at a time, for example, the magnetic separation discs are controlled to rotate two stations at a time, or the magnetic separation discs are controlled to rotate three stations, four stations, etc. at a time. Wherein the rotation direction of the magnetic separation disc is controlled to be clockwise or anticlockwise.

S30: according to the cleaning time sequence of the reaction cup, when the reaction cup moves to the operating mechanism corresponding to the current cleaning time sequence, the operating mechanism is controlled to operate the reaction cup.

According to the cleaning requirement of the reaction cup, a cleaning time sequence is set, and a corresponding operating mechanism is controlled to operate the reaction cup according to the cleaning time sequence, for example, when the reaction cup moves to the liquid discharging mechanism for a certain time, the set cleaning time sequence is required to discharge liquid for the reaction cup, the liquid discharging mechanism is used for carrying out liquid discharging treatment on the reaction cup, after the liquid discharging treatment is finished, the magnetic separation disc is controlled to rotate, the reaction cup moves to the cup grabbing mechanism at the moment, and if the reaction cup needs to be taken out at the set cleaning time sequence, the cup grabbing mechanism is controlled to take out the reaction cup. Therefore, the reaction cup is cleaned by controlling the rotation of the magnetic separation disc and controlling the operation mechanism to match according to the cleaning time sequence of the reaction cup.

In an embodiment, please continue to refer to fig. 5, the total number of the stations of the magnetic separation disc of this embodiment is 11, each station is correspondingly provided with an operating mechanism, and the operating mechanisms sequentially include: a cup grabbing mechanism 501, a liquid preparation mechanism 502, a first magnetic separation mechanism 503, a second magnetic separation mechanism 504, a third magnetic separation mechanism 505, a first liquid discharge mechanism 506, a fourth magnetic separation mechanism 507, a second liquid discharge mechanism 508, a first liquid injection mechanism 509, a fifth magnetic separation mechanism 510, and a second liquid injection mechanism 511.

Wherein, the first liquid injection mechanism 509 and the second liquid injection mechanism 511 can be controlled to add cleaning liquid to the reaction cup or detect substrate.

In the cleaning device of this embodiment, the magnetic separation disc is controlled to rotate three stations each time, and the rotation direction is controlled to be counterclockwise.

Wherein, when the magnetic separation disk rotates first circle, the transfer path of reaction cup is: cup grasping mechanism 501-second magnetic separation mechanism 504-fourth magnetic separation mechanism 507-fifth magnetic separation mechanism 510; when rotating the second circle, the transfer path of the reaction cup is: liquid preparation mechanism 502-third magnetic separation mechanism 505-second liquid discharge mechanism 508-second liquid injection mechanism 511; and when the reaction cup rotates for the third circle, the transfer path of the reaction cup is as follows: first magnetic separation mechanism 503-first drain mechanism 506-first fill mechanism 509-cup grasping mechanism 501.

The first-order cleaning process of the reaction cup by using the cleaning device is approximately as follows: when the reaction cup moves to the liquid preparation mechanism 502 for the second circle, the liquid preparation mechanism 502 is controlled to add cleaning liquid to the liquid in the reaction cup to reach the preset capacity; when the reaction cup moves a second circle to the second liquid discharging mechanism 508, controlling the second liquid discharging mechanism 508 to discharge the liquid in the reaction cup; when the reaction cup moves a second circle to the second liquid injection mechanism 511, controlling the second liquid injection mechanism 511 to inject cleaning liquid into the reaction cup; when the reaction cup moves to the first liquid discharging mechanism 506 for the third circle, controlling the first liquid discharging mechanism 506 to discharge the liquid in the reaction cup; when the cuvette moves to the first liquid-filling mechanism 509 for the third turn, it is judged whether or not to control the first liquid-filling mechanism 509 to fill the detection substrate into the cuvette, and the first liquid-filling mechanism 509 is controlled to fill the detection substrate into the cuvette or not to fill the detection substrate into the cuvette according to the judgment result.

Specifically, referring to fig. 7, fig. 7 is a schematic diagram of a station flowchart illustrating an embodiment of a first-stage cleaning of a reaction cup using the apparatus shown in fig. 5. This embodiment will be described with reference to fig. 6, which shows a first-order cleaning process of the reaction cup. Wherein, the period represents each operation period corresponding to the reaction cup cleaning time sequence, the number of turns represents the number of rotations of the magnetic separation disc, the numbers 501 to 511 are the numbers corresponding to each operation mechanism, and the numbers 1 to 11 corresponding to the cup grabbing mechanism 501 represent the reaction cup to be cleaned which is continuously transferred to the station by the cup grabbing mechanism 501 along with the rotation of the magnetic separation disc.

For cuvette 1, in a first operation cycle, the cuvette handling mechanism 501 is controlled to transfer cuvette 1 to the magnetic separation disc.

Subsequently, in the second operation cycle, the magnetic separation disk is controlled to rotate three stations anticlockwise, the reaction cup 1 is transferred to the second magnetic separation mechanism 504, the cup grabbing mechanism 501 is controlled to transfer the reaction cup 2 to the magnetic separation disk, and the magnetic beads in the reaction cup 1 are adsorbed by the adsorption of the second magnetic separation mechanism 504 and gathered to the end close to the second magnetic separation mechanism 504.

In the third operation period, the magnetic separation disc is controlled to rotate three stations anticlockwise, the reaction cup 1 is transported to the fourth magnetic separation mechanism 507, and the magnetic beads in the reaction cup 1 are adsorbed by the adsorption effect of the fourth magnetic separation mechanism 507 and gathered to the end close to the fourth magnetic separation mechanism 507.

In the fourth operation period, the magnetic separation disc is controlled to rotate three stations anticlockwise, the reaction cup 1 is transported to the fifth magnetic separation mechanism 510, and the magnetic beads in the reaction cup 1 are adsorbed by the adsorption effect of the fifth magnetic separation mechanism 510 and gathered to the end close to the fifth magnetic separation mechanism 510.

In a fifth operation period, the magnetic separation disc is controlled to rotate anticlockwise for three stations, the reaction cup 1 is transported to the liquid distribution mechanism 502, the liquid distribution mechanism 502 is controlled to add cleaning liquid to the reaction cup 1 according to the requirement, so that the liquid in the reaction cup reaches the preset capacity, the cleaning effect of the magnetic beads is enhanced, and if the liquid in the reaction cup reaches the preset capacity, the liquid distribution mechanism 502 is controlled not to perform any operation.

In the sixth operation cycle, the magnetic separation disk is controlled to rotate three stations counterclockwise, the reaction cup 1 is transported to the third magnetic separation mechanism 505, and the magnetic beads in the reaction cup 1 are adsorbed by the adsorption of the third magnetic separation mechanism 505 and gathered to the end close to the third magnetic separation mechanism 505.

In a seventh operation period, the magnetic separation disc is controlled to rotate three stations anticlockwise, the reaction cup 1 is transported to the second liquid discharging mechanism 508, and the second liquid discharging mechanism 508 is controlled to discharge the cleaning liquid in the reaction cup 1.

In the eighth operation period, the magnetic separation disc is controlled to rotate three stations anticlockwise, the reaction cup 1 is transferred to the second liquid injection mechanism 511, and the second liquid injection mechanism 511 is controlled to add cleaning liquid to the reaction cup 1 so as to clean the magnetic beads.

In the ninth operation cycle, the magnetic separation disk is controlled to rotate three stations counterclockwise, the reaction cup 1 is transported to the first magnetic separation mechanism 503, and the magnetic beads in the reaction cup 1 are adsorbed by the adsorption of the first magnetic separation mechanism 503 and gathered to the end close to the first magnetic separation mechanism 503.

In the tenth operation period, the magnetic separation disc is controlled to rotate three stations anticlockwise, the reaction cup 1 is transported to the first liquid draining mechanism 506, and the first liquid draining mechanism 506 is controlled to drain the cleaning liquid in the reaction cup 1.

In the eleventh operation cycle, the magnetic separation plate is controlled to rotate three stations counterclockwise, the cuvette 1 is transferred to the first filling mechanism 509, and whether the first filling mechanism 509 is controlled to add a detection substrate to the cuvette 1 is judged. That is, if the cuvette 1 is to be detected next, the first liquid injection mechanism 509 is controlled to add a detection substrate to the cuvette 1, otherwise, the first liquid injection mechanism 509 does not inject the detection substrate into the cuvette 1.

In the twelfth operation period, the magnetic separation disc is controlled to rotate three stations anticlockwise, the reaction cup 1 is transferred to the cup grabbing mechanism 501, and the cup grabbing mechanism 501 is controlled to take out the reaction cup 1, and here, the reaction cup 1 can be taken out and placed in an optical detection area for optical detection.

Thus, the first-stage cleaning of the cuvette 1 is completed. If there is a reaction cup to be cleaned by magnetic separation, then the cup grabbing mechanism 501 is used for controlling the reaction cup grabbing mechanism to grab the reaction cup to be cleaned in the areas such as the incubation plate after the reaction cup 1 is taken out, adding the reaction cup to the station where the reaction cup 1 is placed, continuously controlling the magnetic separation plate to rotate, and controlling each operating mechanism to perform the reaction cup cleaning operation.

The operations of the reaction cups 2 to 11 in this embodiment are the same as those of the reaction cup 1, and will not be described again.

When this belt cleaning device is used to carry out the second order to the reaction cup and wash, the magnetic separation dish of second order cleaning process rotates 6 circles altogether, and when the magnetic separation dish rotated first circle, the transfer route of reaction cup was: cup grasping mechanism 501-second magnetic separation mechanism 504-fourth magnetic separation mechanism 507-fifth magnetic separation mechanism 510; when rotating the second circle, the transfer path of the reaction cup is: liquid preparation mechanism 502-third magnetic separation mechanism 505-second liquid discharge mechanism 508-second liquid injection mechanism 511; and when the reaction cup rotates for the third circle, the transfer path of the reaction cup is as follows: first magnetic separation mechanism 503-first drain mechanism 506-first priming mechanism 509-cup grasping mechanism 501; when the magnetic separation disc rotates for the fourth circle, the transfer path of the reaction cup is as follows: second magnetic separation mechanism 504-fourth magnetic separation mechanism 507-fifth magnetic separation mechanism 510; when the magnetic separation disc rotates for the fifth circle, the transfer path of the reaction cup is as follows: liquid preparation mechanism 502-third magnetic separation mechanism 505-second liquid discharge mechanism 508-second liquid injection mechanism 511; when rotating the sixth circle, the transfer path of the reaction cup is: first magnetic separation mechanism 503-first drain mechanism 506-first fill mechanism 509-cup grasping mechanism 501.

The second-order cleaning process is approximately as follows: when the reaction cup moves to the liquid preparation mechanism 502 for the second circle, the liquid preparation mechanism 502 is controlled to add cleaning liquid to the liquid in the reaction cup to reach the preset capacity; when the reaction cup moves a second circle to the second liquid discharging mechanism 508, controlling the second liquid discharging mechanism 508 to discharge the liquid in the reaction cup; when the reaction cup moves a second circle to the second liquid injection mechanism 511, controlling the second liquid injection mechanism 511 to inject cleaning liquid into the reaction cup; when the reaction cup moves to the first liquid discharging mechanism 506 for the third circle, controlling the first liquid discharging mechanism 506 to discharge the liquid in the reaction cup; when the reaction cup moves to the first liquid injection mechanism 509 for the third circle, controlling the first liquid injection mechanism 509 to inject cleaning liquid into the reaction cup; when the reaction cup moves to the first liquid discharging mechanism 506 for the sixth circle, controlling the first liquid discharging mechanism 506 to discharge the liquid in the reaction cup; when the reaction cup moves to the first liquid injection mechanism 509 for the sixth turn, it is judged whether the first liquid injection mechanism 509 is controlled to inject the detection substrate into the reaction cup, if the detection operation is to be performed on the reaction cup, the first liquid injection mechanism 509 is controlled to inject the detection substrate into the reaction cup, otherwise, the first liquid injection mechanism 509 does not inject the substrate into the reaction cup.

Specifically, referring to fig. 8, fig. 8 is a schematic diagram of a station in which the apparatus shown in fig. 5 is used to perform a second-order cleaning of the reaction cup. This embodiment will be described with reference to fig. 6, which shows a second-order cleaning process of the reaction cup.

In the present embodiment, during the first to tenth operation periods, the operation of each mechanism is the same as the first-order cleaning process, and no further description is given.

In the eleventh operation cycle, the magnetic separation plate is controlled to rotate three stations counterclockwise, the reaction cup 1 is transferred to the first liquid injection mechanism 509, and the first liquid injection mechanism 509 is controlled to add the cleaning liquid to the reaction cup 1.

In the twelfth operation period, the magnetic separation plate is controlled to rotate three stations anticlockwise, the reaction cup 1 is transferred to the cup grabbing mechanism 501, at this time, the cup grabbing mechanism 501 does not perform any operation, the reaction cup 1 is not taken out, and the reaction cup is still remained on the magnetic separation plate.

In the thirteenth operation period, the magnetic separation disk is controlled to rotate three stations anticlockwise, the reaction cup 1 is transferred to the second magnetic separation mechanism 504, the cup grabbing mechanism 501 is controlled to transfer the reaction cup 2 to the magnetic separation disk, and the magnetic beads in the reaction cup 1 are adsorbed by the adsorption of the second magnetic separation mechanism 504 and gathered to the end close to the second magnetic separation mechanism 504.

In the fourteenth operation period, the magnetic separation disc is controlled to rotate three stations anticlockwise, the reaction cup 1 is transported to the fourth magnetic separation mechanism 507, and the magnetic beads in the reaction cup 1 are adsorbed by the adsorption effect of the fourth magnetic separation mechanism 507 and gathered to the end close to the fourth magnetic separation mechanism 507.

In the fifteenth operation period, the magnetic separation disk is controlled to rotate three stations anticlockwise, the reaction cup 1 is transported to the fifth magnetic separation mechanism 510, and the magnetic beads in the reaction cup 1 are adsorbed by the adsorption effect of the fifth magnetic separation mechanism 510 and gathered to the end close to the fifth magnetic separation mechanism 510.

In the sixteenth operation cycle, the magnetic separation plate is controlled to rotate three stations counterclockwise, the cuvette 1 is transferred to the liquid dispensing mechanism 502, and the liquid dispensing mechanism 502 does not operate the cuvette 1.

In the seventeenth operation cycle, the magnetic separation disk is controlled to rotate three stations counterclockwise, the reaction cup 1 is transferred to the third magnetic separation mechanism 505, and the magnetic beads in the reaction cup 1 are adsorbed by the adsorption of the third magnetic separation mechanism 505 and gathered to the end close to the third magnetic separation mechanism 505.

In the eighteenth operation cycle, the magnetic separation plate is controlled to rotate three stations counterclockwise, the reaction cup 1 is transferred to the second liquid discharging mechanism 508, and the second liquid discharging mechanism 508 does not perform any operation on the reaction cup 1.

In the nineteenth operation cycle, the magnetic separation plate is controlled to rotate three stations counterclockwise, the cuvette 1 is transferred to the second liquid-filling mechanism 511, and the second liquid-filling mechanism 511 does not perform any operation on the cuvette 1.

In the twentieth operation period, the magnetic separation disk is controlled to rotate three stations counterclockwise, the reaction cup 1 is transported to the first magnetic separation mechanism 503, and the magnetic beads in the reaction cup 1 are adsorbed by the adsorption of the first magnetic separation mechanism 503 and gathered to the end close to the first magnetic separation mechanism 503.

In the twenty-first operation period, the magnetic separation disc is controlled to rotate three stations anticlockwise, the reaction cup 1 is transferred to the first liquid discharging mechanism 506, the first liquid discharging mechanism 506 is controlled to discharge the cleaning liquid in the reaction cup 1, and second-order cleaning is completed.

In the twenty-second operation cycle, the magnetic separation plate is controlled to rotate three stations counterclockwise, the cuvette 1 is transferred to the first filling mechanism 509, and whether the first filling mechanism 509 is controlled to add a detection substrate to the cuvette 1 is judged. That is, if the cuvette 1 is to be detected next, the first liquid injection mechanism 509 is controlled to add a detection substrate to the cuvette 1, otherwise, the first liquid injection mechanism 509 does not inject the detection substrate into the cuvette 1.

In a twenty-third operation period, the magnetic separation disc is controlled to rotate three stations anticlockwise, the reaction cup 1 is transferred to the cup grabbing mechanism 501, and the cup grabbing mechanism 501 is controlled to take out the reaction cup 1, and here, the reaction cup 1 can be taken out and placed in an optical detection area for optical detection. Thus, the second-order cleaning of the cuvette 1 is completed.

Or in the process, in the eighteenth operation period, the magnetic separation disc is controlled to rotate three stations anticlockwise, the reaction cup 1 is transferred to the second liquid draining mechanism 508, and the second liquid draining mechanism 508 is controlled to drain the cleaning liquid in the reaction cup 1; in the nineteenth operation period, the magnetic separation disc is controlled to rotate three stations anticlockwise, the reaction cup 1 is transferred to the second liquid injection mechanism 511, whether the second liquid injection mechanism 511 is controlled to add detection substrates to the reaction cup 1 is judged, if the reaction cup 1 is to be detected next, the second liquid injection mechanism 511 is controlled to add detection substrates to the reaction cup 1, otherwise, the second liquid injection mechanism 511 does not inject detection substrates to the reaction cup 1. In the twenty-first operation cycle and the twenty-second operation cycle, the first drain mechanism 506 and the first filling mechanism 509 do not perform any operation on the cuvette 1.

When the cleaning device is required to be used for carrying out third-order cleaning on the reaction cup, the magnetic separation disc rotates for 6 circles in total in the third-order cleaning process, and the transfer path of the reaction cup is the same as that of the second-order cleaning reaction cup, so that the description is omitted.

The three-stage cleaning process is approximately as follows: when the reaction cup moves to the liquid preparation mechanism 502 for the second circle, the liquid preparation mechanism 502 is controlled to add cleaning liquid to the liquid in the reaction cup to reach the preset capacity; when the reaction cup moves a second circle to the second liquid discharging mechanism 508, controlling the second liquid discharging mechanism 508 to discharge the liquid in the reaction cup; when the reaction cup moves a second circle to the second liquid injection mechanism 511, controlling the second liquid injection mechanism 511 to inject cleaning liquid into the reaction cup; when the reaction cup moves to the first liquid discharging mechanism 506 for the third circle, controlling the first liquid discharging mechanism 506 to discharge the liquid in the reaction cup; when the reaction cup moves to the first liquid injection mechanism 509 for the third circle, controlling the first liquid injection mechanism 509 to inject cleaning liquid into the reaction cup; when the reaction cup moves from the fifth circle to the second liquid discharging mechanism 508, controlling the second liquid discharging mechanism 508 to discharge the liquid in the reaction cup; when the reaction cup moves a fifth circle to the second liquid injection mechanism 511, controlling the second liquid injection mechanism 511 to inject cleaning liquid into the reaction cup; when the reaction cup moves to the first liquid discharging mechanism 506 for the sixth circle, controlling the first liquid discharging mechanism 506 to discharge the liquid in the reaction cup; when the reaction cup moves to the first liquid injection mechanism 509 for the sixth turn, it is judged whether the first liquid injection mechanism 509 is controlled to inject the detection substrate into the reaction cup, if the detection operation is to be performed on the reaction cup, the first liquid injection mechanism 509 is controlled to inject the detection substrate into the reaction cup, otherwise, the first liquid injection mechanism 509 does not inject the substrate into the reaction cup.

Specifically, referring to fig. 9, fig. 9 is a schematic diagram of a station flowchart illustrating an embodiment of three-stage cleaning of a reaction cup using the apparatus shown in fig. 5. The operation cycle of the embodiment is the same as the operation cycle of the second-order cleaning, and the difference is that during the third-order cleaning, in the eighteenth operation cycle, the magnetic separation disc is controlled to rotate three stations anticlockwise, the reaction cup 1 is transferred to the second liquid discharging mechanism 508, the second liquid discharging mechanism 508 is controlled to discharge the cleaning liquid in the reaction cup 1, and the second-order cleaning is completed; in the nineteenth operation period, the magnetic separation disc is controlled to rotate three stations anticlockwise, the reaction cup 1 is transferred to the second liquid injection mechanism 511, the second liquid injection mechanism 511 is controlled to add cleaning liquid to the reaction cup 1, in the twenty first operation period, the magnetic separation disc is controlled to rotate three stations anticlockwise, the reaction cup 1 is transferred to the first liquid discharge mechanism 506, the first liquid discharge mechanism 506 is controlled to discharge the cleaning liquid in the reaction cup 1, and third-order cleaning is completed; in the twenty-second operation cycle, the magnetic separation disk is controlled to rotate three stations anticlockwise, the reaction cup 1 is transported to the first liquid injection mechanism 509, whether the first liquid injection mechanism 509 is controlled to add detection substrates to the reaction cup 1 is judged, if the reaction cup 1 is detected next, the first liquid injection mechanism 509 is controlled to add detection substrates to the reaction cup 1, otherwise, the first liquid injection mechanism 509 does not inject detection substrates to the reaction cup 1. The rest operation period is the same as the operation of the second-order cleaning corresponding operation period shown in fig. 7, and will not be described again.

If more steps of cleaning are needed, the second-order cleaning and the third-order cleaning processes can be imitated, and the operation mechanisms are controlled to be matched for operation.

Wherein, magnetic separation mechanisms can be arranged at the corresponding stations of part or all of the operating mechanisms in the cup grabbing mechanism 501, the liquid preparing mechanism 502, the first liquid discharging mechanism 506, the second liquid discharging mechanism 508, the first liquid injecting mechanism 509 and the second liquid injecting mechanism 511 according to the requirement, so that when the operating mechanisms of the magnetic separation mechanisms are arranged in the matching way, the magnet in the magnetic separation mechanisms can absorb magnetic beads, and then the magnetic beads are scattered or gathered. For example, magnetic separation mechanisms are arranged at corresponding stations of the first liquid discharge mechanism 506 and the second liquid discharge mechanism 508 in a matched manner, when the reaction cup reaches the first liquid discharge mechanism 506, magnetic beads are adsorbed and gathered on the inner wall of the reaction cup due to the adsorption action of a magnet in the magnetic separation mechanism, and meanwhile, the first liquid discharge mechanism 506 discharges cleaning liquid in the reaction cup, so that the magnetic beads are prevented from being discharged by mistake when the first liquid discharge mechanism 506 performs liquid discharge operation.

In an embodiment, the cup grabbing mechanism 501, the liquid dispensing mechanism 502, the first liquid draining mechanism 506, the second liquid draining mechanism 508, the first liquid injecting mechanism 509 and the second liquid injecting mechanism 511 are all provided with magnetic separating mechanisms in a matched manner, so that when the reaction cup is transported to the corresponding station of each operation mechanism, the magnetic beads can be adsorbed. For example, referring to fig. 10, each of the operating mechanisms of the present embodiment is provided with a magnetic separation mechanism, and the magnet arrangement positions of each magnetic separation mechanism may be arranged in opposite directions on adjacent transfer stations according to the transfer path of the reaction cup. The adjacent transferring stations are two stations which are sequentially reached by the reaction cup under the drive of the continuous twice rotation of the magnetic separation disc.

For example, the transfer path of the reaction cup of this embodiment is: the cup grabbing mechanism 501-the second magnetic separation mechanism 504-the fourth magnetic separation mechanism 507-the fifth magnetic separation mechanism 510-the liquid preparation mechanism 502-the third magnetic separation mechanism 505-the second liquid draining mechanism 508-the second liquid injecting mechanism 511-the first magnetic separation mechanism 503-the first liquid draining mechanism 506-the first liquid injecting mechanism 509-the cup grabbing mechanism 501. According to this transfer path, the magnet corresponding to the cup grasping mechanism 501 is disposed inside the transfer path, the magnet corresponding to the second magnetic separation mechanism 504 is disposed outside the transfer path, the magnet corresponding to the fourth magnetic separation mechanism 507 is disposed inside the transfer path, the magnet corresponding to the fifth magnetic separation mechanism 510 is disposed outside the transfer path, the magnet corresponding to the liquid dispensing mechanism 502 is disposed inside the transfer path, the magnet corresponding to the third magnetic separation mechanism 505 is disposed outside the transfer path, the magnet corresponding to the second liquid discharging mechanism 508 is disposed inside the transfer path, the magnet corresponding to the second liquid filling mechanism 511 is disposed outside the transfer path, the magnet corresponding to the first magnetic separation mechanism 503 is disposed inside the transfer path, the magnet corresponding to the first liquid discharging mechanism 506 is disposed outside the transfer path, and the magnet corresponding to the first liquid filling mechanism 509 is disposed inside the transfer path. Therefore, the reaction cup can be sequentially adsorbed to the inner wall of the reaction cup close to the inner side of the transfer path and the inner wall of the reaction cup close to the outer side of the transfer path in the continuous and repeated transfer process, so that the magnetic beads can be thoroughly cleaned. The arrangement position of the magnet in the magnetic separation mechanism can be in other various ways, but is not limited to the above.

Referring to fig. 11, the present application further provides a sample analysis device 60, where the sample analysis device 60 includes: the reaction cup cleaning device comprises a magnetic separation disc 61, a plurality of operating mechanisms 62, a memory 63 and a controller 64, wherein a plurality of stations are arranged on the magnetic separation disc 61 and used for placing the reaction cup, and the operating mechanisms 62 are arranged in one-to-one correspondence with the stations and used for operating the reaction cup placed at the corresponding station so as to finish cleaning of the reaction cup.

The memory 63 is used for storing program data, and the controller 64 is connected to the memory 63, the magnetic separation disk 61 and the plurality of operating mechanisms 62 for executing the program data to realize the cleaning method of the magnetic separation reaction cup as described in the above embodiments.

For the description of each step of the processing execution, please refer to the description of each step of the cleaning method embodiment of the magnetic separation reaction cup in the present application, and the description is omitted herein. The sample analyzer 60 is, for example, a chemo-optical immunoassay analyzer.

In the embodiments of the present application, the disclosed method for cleaning a magnetic separation cuvette and sample analysis device may be implemented in other ways. For example, the various embodiments of the sample analysis device described above are merely illustrative, e.g., the division of the modules or units is merely a logical functional division, and there may be additional divisions in actual implementation, e.g., multiple units or components may be combined or integrated into another system, or some features may be omitted, or not performed. Alternatively, the coupling or direct coupling or communication connection shown or discussed with each other may be an indirect coupling or communication connection via some interfaces, devices or units, which may be in electrical, mechanical or other form.

The units described as separate units may or may not be physically separate, and units shown as units may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the units may be selected according to actual needs to achieve the purpose of the embodiment.

In addition, each functional unit in each embodiment of the present application may be integrated in one processing unit, or each unit may exist alone physically, or two or more units may be integrated in one unit. The integrated units may be implemented in hardware or in software functional units.

The integrated units, if implemented in the form of software functional units and sold or used as stand-alone products, may be stored in a computer readable storage medium. Based on such understanding, the technical solution of the present application may be embodied essentially or partly contributing to the prior art or in whole or in part in the form of a software product, which is stored in a storage medium.

Referring to fig. 12, fig. 12 is a schematic block diagram illustrating a circuit structure of an embodiment of a computer readable storage medium of the present application, where the computer storage medium 1000 stores a computer program 1001, and the computer program 1001 implements the steps of the method for cleaning a magnetic separation cuvette according to the present application.

The computer storage medium 1000 may be a usb disk, a removable hard disk, a Read-Only Memory (ROM), a random access Memory (RAM, random Access Memory), a magnetic disk, or an optical disk, etc. various media capable of storing program codes.

The foregoing description is only exemplary embodiments of the present application and is not intended to limit the scope of the present application, and all equivalent structures or equivalent processes using the descriptions and the drawings of the present application, or direct or indirect application in other related technical fields are included in the scope of the present application.

Claims

1. A cleaning device for a magnetic separation cuvette, the cleaning device comprising:

the magnetic separation disc is provided with a plurality of stations for placing reaction cups;

the operating mechanisms are arranged in one-to-one correspondence with the stations;

the magnetic separation disc rotates at least two stations each time so as to control the corresponding operating mechanism to operate the reaction cup when the reaction cup moves to the corresponding operating mechanism according to the cleaning time sequence of the reaction cup on each station, wherein the number of the working sites rotated each time is smaller than the total number of the working sites, and the integral multiple of the number of the working sites rotated each time is unequal to the total number of the working sites;

Wherein, operating device includes:

a plurality of magnetic separation mechanisms for performing magnetic separation operation on the liquid in the reaction cup;

a plurality of liquid discharging mechanisms for discharging the liquid in the reaction cup;

the liquid injection mechanisms are used for injecting cleaning liquid or detecting substrates into the reaction cup after liquid discharge; the magnetic separation mechanism is arranged between two adjacent liquid discharge mechanisms, one magnetic separation mechanism is arranged between two adjacent liquid injection mechanisms, and the number of the working bits per rotation is 3, so that each reaction cup needing liquid injection or liquid discharge can be adsorbed, gathered and scattered through the magnetic separation mechanism;

the cup grabbing mechanism is used for placing the reaction cup on the station or taking the reaction cup off the station;

the liquid preparation mechanism is used for judging the liquid amount in the reaction cup so as to determine whether the preset capacity is reached, and if the preset capacity is not reached, adding cleaning liquid into the liquid in the reaction cup to reach the preset capacity;

the magnet arrangement positions of the magnetic separation mechanisms are arranged in opposite directions on adjacent transfer stations according to the transfer paths of the reaction cups; wherein, adjacent transfer stations are: the reaction cup is driven by the magnetic separation disc to rotate twice continuously to sequentially reach two stations.

2. The cleaning apparatus of claim 1, wherein the cleaning apparatus comprises a cleaning device,

the total number of the work stations is 11;

the operating mechanism sequentially comprises the following components according to the rotation direction of the magnetic separation disc: the device comprises a cup grabbing mechanism, a liquid distribution mechanism, a first magnetic separation mechanism, a second magnetic separation mechanism, a third magnetic separation mechanism, a first liquid discharging mechanism, a fourth magnetic separation mechanism, a second liquid discharging mechanism, a first liquid injection mechanism, a fifth magnetic separation mechanism and a second liquid injection mechanism.

3. A method for cleaning a magnetic separation cuvette, characterized in that the method is applied to a cleaning device according to any one of claims 1-2, the method comprising:

the cup grabbing mechanism is controlled to place the reaction cup to be cleaned on a corresponding station of the magnetic separation disc or take off the reaction cup from the station;

controlling the magnetic separation disc to rotate three stations each time;

according to the cleaning time sequence of the reaction cup, when the reaction cup moves to an operating mechanism corresponding to the current cleaning time sequence, controlling the operating mechanism to operate the reaction cup; comprising the following steps: when the reaction cup moves to a station corresponding to the liquid distribution mechanism, controlling the liquid distribution mechanism to judge the liquid amount in the reaction cup so as to determine whether the preset capacity is reached, and if the preset capacity is not reached, adding cleaning liquid into the liquid in the reaction cup to the preset capacity; when the reaction cup moves to a station corresponding to the liquid discharge mechanism, controlling the liquid discharge mechanism to discharge liquid in the reaction cup; when the reaction cup moves to the liquid injection mechanism, the liquid injection mechanism is controlled to inject cleaning liquid or detection substrate into the reaction cup after liquid discharge.

4. The method of claim 3, wherein the step of,

the total number of the work stations is 11;

the operating mechanism sequentially comprises the following components according to the rotation direction of the magnetic separation disc: the device comprises a cup grabbing mechanism, a liquid distribution mechanism, a first magnetic separation mechanism, a second magnetic separation mechanism, a third magnetic separation mechanism, a first liquid discharging mechanism, a fourth magnetic separation mechanism, a second liquid discharging mechanism, a first liquid injection mechanism, a fifth magnetic separation mechanism and a second liquid injection mechanism;

the controlling the magnetic separation disc to rotate at least two stations each time comprises the following steps:

and controlling the magnetic separation disc to rotate three stations at a time.

5. The method of claim 4, wherein the step of determining the position of the first electrode is performed,

according to the cleaning time sequence of the reaction cup, when the reaction cup moves to an operating mechanism corresponding to the current cleaning time sequence, the operating mechanism is controlled to operate the reaction cup, and the method comprises the following steps:

when the reaction cup moves a second circle to the liquid distribution mechanism, controlling the liquid distribution mechanism to add cleaning liquid into the liquid in the reaction cup to a preset capacity;

when the reaction cup moves a second circle to the second liquid draining mechanism, controlling the second liquid draining mechanism to drain the liquid in the reaction cup;

When the reaction cup moves a second circle to the second liquid injection mechanism, controlling the second liquid injection mechanism to inject cleaning liquid into the reaction cup;

when the reaction cup moves a third circle to the first liquid draining mechanism, controlling the first liquid draining mechanism to drain liquid in the reaction cup;

when the reaction cup moves for the third circle to the first liquid injection mechanism, judging whether to control the first liquid injection mechanism to inject detection substrates into the reaction cup.

6. The method of claim 4, wherein the controlling the operating mechanism to operate the cuvette when the cuvette moves to the operating mechanism corresponding to the current cleaning timing according to the cleaning timing of the cuvette, comprises:

when the reaction cup moves a third circle to the first liquid injection mechanism, controlling the first liquid injection mechanism to inject cleaning liquid into the reaction cup;

when the reaction cup moves a sixth circle to the first liquid draining mechanism, controlling the first liquid draining mechanism to drain the liquid in the reaction cup;

and when the reaction cup moves for the sixth circle to the first liquid injection mechanism, judging whether to control the first liquid injection mechanism to inject detection substrates into the reaction cup.

7. The method of claim 4, wherein the controlling the operating mechanism to operate the cuvette when the cuvette moves to the operating mechanism corresponding to the current cleaning timing according to the cleaning timing of the cuvette, comprises:

when the reaction cup moves a fifth circle to the second liquid draining mechanism, controlling the second liquid draining mechanism to drain the liquid in the reaction cup;

when the reaction cup moves for a fifth circle to the second liquid injection mechanism, controlling the second liquid injection mechanism to inject cleaning liquid into the reaction cup;

8. A sample analysis device, the sample analysis device comprising:

a memory for storing program data;

a controller coupled to said memory, said magnetic separation disk and said plurality of operating mechanisms for executing said program data to implement the method of any of claims 3-7.