CN114618850B - Method for cleaning magnetic separation cuvette, sample analyzer, and readable storage medium - Google Patents
Method for cleaning magnetic separation cuvette, sample analyzer, and readable storage medium Download PDFInfo
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- CN114618850B CN114618850B CN202011459431.6A CN202011459431A CN114618850B CN 114618850 B CN114618850 B CN 114618850B CN 202011459431 A CN202011459431 A CN 202011459431A CN 114618850 B CN114618850 B CN 114618850B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/20—Cleaning containers, e.g. tanks by using apparatus into or on to which containers, e.g. bottles, jars, cans are brought
- B08B9/22—Cleaning containers, e.g. tanks by using apparatus into or on to which containers, e.g. bottles, jars, cans are brought the apparatus cleaning by soaking alone
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/20—Cleaning containers, e.g. tanks by using apparatus into or on to which containers, e.g. bottles, jars, cans are brought
- B08B9/42—Cleaning containers, e.g. tanks by using apparatus into or on to which containers, e.g. bottles, jars, cans are brought the apparatus being characterised by means for conveying or carrying containers therethrough
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Abstract
The application discloses a cleaning method of a magnetic separation reaction cup, a sample analysis device and a computer readable storage medium, wherein the cleaning method comprises the following steps: obtaining a reaction cup to be cleaned; wherein, the reaction cup is internally provided with a reaction liquid; controlling a liquid preparation mechanism to perform liquid preparation operation on the reaction cup so that the liquid level height in the reaction cup is larger than a preset height; controlling a liquid discharge mechanism to perform liquid discharge operation on the reaction cup; and controlling the liquid injection mechanism to perform liquid injection operation on the reaction cup. Through the mode, the cleaning effect of the magnetic beads can be enhanced, and the recovery rate of the magnetic beads can be improved.
Description
Technical Field
The present invention relates to the field of medical detection technology, and in particular, to a method for cleaning a magnetic separation cuvette, a sample analyzer, and a readable storage medium.
Background
The chemical immunoassay technology is mainly a means for detecting by utilizing the specific reaction of an antigen and an antibody, and is often used for detecting trace substances such as proteins and hormones because detection signals can be amplified and displayed by isotopes, enzymes, chemiluminescent substances and the like.
Magnetic bead separation is an important step in chemical immunoassays, and magnetic beads are magnetic microspheres coated with specific biomolecules that can specifically bind to a target substance containing the same to form a new complex, which can be retained by a magnetic field and separated from other components. The method is mainly applied to protein purification, cell separation and the like. The core of the magnetic bead technology is that a proper external magnetic field is adopted to magnetize and adsorb the magnetic beads on the inner wall of the reaction cup, when the external magnetic field is removed, the magnetism of the magnetic beads disappears, the magnetic beads are dispersed in the solution again, and the magnetic beads are scattered and gathered continuously by utilizing the characteristic, so that the magnetic beads are cleaned.
The sample amount and the reagent adding total amount are different due to different detection items, so that the liquid level of the reaction cup is inconsistent when the reaction cup is placed in the magnetic separation disc, and the height of a magnet outside the magnetic separation disc is fixed, so that the effect of the magnet for adsorbing the magnetic beads of the reaction cup is affected, the first-order cleaning effect is poor, the magnetic loss rate is high, and the recovery rate of the magnetic beads is low.
Disclosure of Invention
The application mainly provides a cleaning method of a magnetic separation reaction cup, a sample analysis device and a readable storage medium, which can solve the problems of low recovery rate of magnetic beads and poor cleaning effect of the magnetic beads in the prior art.
In order to solve the above technical problems, a first aspect of the present application provides a method for cleaning a magnetic separation reaction cup, which includes: obtaining a reaction cup to be cleaned; wherein, the reaction cup is internally provided with a reaction liquid; controlling a liquid preparation mechanism to perform liquid preparation operation on the reaction cup so that the liquid level height in the reaction cup is larger than a preset height; controlling a liquid discharge mechanism to perform liquid discharge operation on the reaction cup; and controlling the liquid injection mechanism to perform liquid injection operation on the reaction cup.
After liquid preparation, the reaction cup is subjected to magnetic separation operation by utilizing a magnetic separation mechanism, and the preset height is more than or equal to 1/3 of the height of a magnet in the magnetic separation mechanism.
When the reaction cup rotates to the liquid preparation level, the liquid preparation mechanism is controlled to perform liquid preparation operation on the reaction cup, so that the liquid level in the reaction cup reaches the preset height, and the method comprises the following steps: acquiring the current liquid level height of the reaction cup; judging whether the current liquid level height is larger than the preset height; if not, controlling a liquid preparation mechanism to perform liquid preparation operation on the reaction cup so as to enable the liquid level in the reaction cup to reach a preset height.
Wherein, the obtaining the current liquid level height of the reaction cup comprises: and detecting the liquid level of the reaction cup to obtain the current liquid level height of the reaction cup.
Wherein, the obtaining the current liquid level height of the reaction cup comprises: acquiring detection items corresponding to the reaction liquid in the reaction cup; and determining the current liquid level height of the reaction cup according to the detection item.
Wherein, control liquid preparation mechanism is carried out liquid preparation operation to the reaction cup, so that liquid level height in the reaction cup reaches the preset height and includes: determining liquid preparation capacity according to the current liquid level height and the preset liquid level height; and controlling a liquid distribution mechanism to perform liquid distribution operation on the reaction cup according to the liquid distribution capacity so as to enable the liquid level height in the reaction cup to reach a preset height.
Wherein, control liquid preparation mechanism is operated to the reaction cup is joined in marriage liquid, includes: placing the reaction cup on a magnetic separation disc; controlling the magnetic separation disc to rotate to the liquid distribution level; controlling a liquid preparation mechanism to perform liquid preparation operation on the reaction cup; the control liquid injection mechanism performs liquid injection operation on the reaction cup, and comprises the following steps: controlling the magnetic separation disc to rotate to the liquid injection level; controlling a liquid injection mechanism to perform liquid injection operation on the reaction cup; the liquid injection mechanism is connected with the liquid injection needle, and the liquid injection mechanism is connected with the liquid injection needle.
Wherein, control liquid preparation mechanism is operated to the reaction cup is joined in marriage liquid, includes: placing the reaction cup on a magnetic separation disc; controlling the magnetic separation disc to rotate to the liquid distribution level; controlling a liquid preparation mechanism to perform liquid preparation operation on the reaction cup; the control liquid injection mechanism performs liquid injection operation on the reaction cup, and comprises the following steps: moving the cuvette from the magnetic separation disc to an optical detection position; controlling a liquid injection mechanism to perform liquid injection operation on the reaction cup; the liquid preparation mechanism and the liquid injection mechanism adopt the same liquid injection needle to complete liquid injection, and the liquid injection needle can move between the magnetic separation disc and the optical detection position.
To solve the above technical problem, a second aspect of the present application provides a sample analysis device, which includes a controller and a memory, where program data is stored in the memory, and the program data, when executed by the controller, implements the method for cleaning a magnetic separation reaction cup provided in the first aspect.
To solve the above technical problem, a third aspect of the present application provides a computer readable storage medium having stored therein program data, which when executed by a controller, is configured to implement the method for cleaning a magnetic separation cuvette provided in the first aspect.
The beneficial effects of this application are: the reaction cup that is different from prior art's condition is through acquireing to this application to control liquid mechanism and carry out the operation of joining in marriage liquid to the reaction cup, so that the liquid level height in the reaction cup is greater than the default height, control liquid discharging mechanism carries out the operation of flowing back to the reaction cup afterwards, and, control annotate liquid mechanism and carry out the operation of annotating liquid to the reaction cup, in order to accomplish the washing of reaction cup. The liquid level of the reaction liquid of the reaction cup is different due to the difference of detection items, and the liquid distribution mechanism is used for distributing the liquid of the reaction cup, so that the liquid level of the reaction cup reaches the preset height, and the magnetic beads can move along with the liquid in the reaction cup and adapt to the height of the magnetic separation mechanism when being adsorbed and gathered by the magnetic separation mechanism, thereby improving the adsorption effect of the magnetic beads, enhancing the cleaning effect and improving the recovery rate of the magnetic beads.
Drawings
FIG. 1 is a schematic diagram of an embodiment of a cleaning apparatus for magnetic separation reaction cups of the present application;
FIG. 2 is a diagram showing the relative positional relationship between the magnetic separation mechanism and the reaction cup according to an embodiment of the present invention;
FIG. 3 is a schematic block diagram of a process flow of one embodiment of a method of cleaning a magnetic separation cuvette in accordance with the present application;
FIG. 4 is a schematic diagram showing the effect of the magnetic separation mechanism on the adsorption and aggregation of magnetic beads after liquid preparation;
FIG. 5 is a schematic view of another embodiment of a magnetic separation mechanism and magnet relationship of the present application;
FIG. 6 is a schematic block diagram of a process for preparing a liquid from a reaction cup in step S20 of the present application;
FIG. 7 is a schematic block diagram illustrating a flowchart of an embodiment of step S203 of the present application;
FIG. 8 is a schematic view of an embodiment of a cleaning apparatus for magnetic separation reaction cups according to the present application;
FIG. 9 is a schematic view of another embodiment of a cleaning apparatus for magnetic separation reaction cups of the present application;
FIG. 10 is a block diagram schematically illustrating an embodiment of a sample analyzer according to the present application;
FIG. 11 is a schematic diagram of the structure of an embodiment of the cleaning apparatus for magnetic separation reaction cups of the present application;
FIG. 12 is a schematic block diagram of a circuit configuration of an embodiment of a sample analysis device of the present application;
fig. 13 is a schematic block diagram of a circuit configuration of an embodiment of a computer-readable storage medium of the present application.
Detailed Description
The following description of the technical solutions in the embodiments of the present application will be made clearly and completely with reference to the accompanying drawings in the embodiments of the present application, and it is apparent that the described embodiments are only some embodiments of the present application, not all embodiments. All other embodiments, which can be made by one of ordinary skill in the art without undue burden from the present disclosure, are within the scope of the present disclosure.
The terms "first," "second," and the like in this application are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of features shown. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. Furthermore, the terms "comprise" and "have," as well as any variations thereof, are intended to cover a non-exclusive inclusion. For example, a process, method, system, article, or apparatus that comprises a list of steps or elements is not limited to only those listed steps or elements but may include other steps or elements not listed or inherent to such process, method, article, or apparatus.
Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment may be included in at least one embodiment of the present application. The appearances of such phrases in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. Those of skill in the art will explicitly and implicitly understand that the embodiments described herein may be combined with other embodiments.
Referring to fig. 1, fig. 1 is a schematic diagram illustrating an embodiment of a cleaning apparatus for magnetic separation reaction cups for cleaning incubated magnetic beads to remove free interfering substances therein. The cleaning device comprises a magnetic separation disc 10 and a plurality of operating mechanisms, wherein the magnetic separation disc 10 is provided with a plurality of stations 101 for placing reaction cups, and the stations are arranged in one-to-one correspondence with the operating mechanisms so as to operate the reaction cups placed at the corresponding stations 101 through the operating mechanisms. Specifically, the operating mechanism is used for performing operations such as liquid injection, liquid discharge, magnetic attraction and the like on the reaction cup, so as to clean the magnetic beads. Specifically, the plurality of operating mechanisms includes: the device comprises a magnetic separation mechanism, a liquid discharge mechanism, a liquid injection mechanism, a cup grabbing mechanism and a liquid distribution mechanism, wherein the magnetic separation mechanism is used for carrying out magnetic separation operation on liquid in a reaction cup, the liquid discharge mechanism is used for discharging the liquid in the reaction cup, the liquid injection mechanism is used for injecting cleaning liquid or detecting substrates in the reaction cup after liquid discharge, the cup grabbing mechanism is used for placing the reaction cup on a station or removing the reaction cup from the station, and the liquid distribution mechanism is used for adding the cleaning liquid into the liquid in the reaction cup to reach a preset capacity.
With continued reference to fig. 1, a dashed line 102 in fig. 1 is a transfer path of the reaction cup, where two sides of the transfer path exist, and the magnetic separation mechanism may be disposed on one side of the transfer path 102 and opposite to a bottom of the reaction cup, so that when the reaction cup is transferred to the magnetic separation mechanism, the magnetic beads in the reaction cup are adsorbed, and move to a side close to the magnetic piece under the adsorption of the magnetic piece and gather. For example, the magnetic separation mechanisms 201 and 202 are arranged in two different ways, when the reaction cup moves to the position of the magnetic separation mechanism 201, the magnetic beads are gathered in the direction approaching the magnetic separation mechanism 201, and when the reaction cup moves to the position of the magnetic separation mechanism 202, the magnetic beads are gathered in the direction approaching the magnetic separation mechanism 202.
After the incubation is finished, the cup grabbing mechanism is controlled to move the reaction cup from the incubation area to the magnetic separation disc, and after the washing is finished, the cup grabbing mechanism is controlled to move the reaction cup from the magnetic separation disc to the detection area for detection.
Referring to fig. 2, fig. 2 is a schematic diagram illustrating an embodiment of a relative positional relationship between the magnetic separation mechanism and the reaction cup. The magnetic separation mechanism 200 is a magnet, and the magnet is disposed at a side of a station where the reaction cup is placed, and is disposed opposite to the bottom of the reaction cup 100, so as to adsorb and collect magnetic beads in the reaction cup 100.
The method for cleaning the magnetic separation cuvette will be described based on the apparatus for cleaning a magnetic separation cuvette provided in fig. 1.
Referring to fig. 3, fig. 3 is a schematic flow chart diagram illustrating an embodiment of a method for cleaning a magnetic separation cuvette according to the present application. The cleaning method of the embodiment comprises the following steps:
s10: and obtaining the reaction cup to be cleaned.
The step controls the cup grabbing mechanism to place the reaction cup to be cleaned at the corresponding station of the magnetic separation disc.
Specifically, after the reaction cup to be cleaned is a reaction cup which is incubated in the areas such as an incubation plate after the reaction liquid and the sample liquid are added, the reaction cup grabbing mechanism is controlled to take out the reaction cup to be cleaned from the areas such as the incubation plate after the incubation of the reaction cup is completed, the reaction cup is placed at a station of the magnetic separation plate corresponding to the reaction cup grabbing mechanism, and the reaction cup is filled with the reaction liquid.
S20: the liquid distribution mechanism is controlled to perform liquid distribution operation on the reaction cup, so that the liquid level height in the reaction cup is larger than the preset height.
The step controls the liquid distribution mechanism to distribute liquid in the reaction cup to be cleaned obtained in the step S10 according to the requirement, so that the liquid level height in the reaction cup is larger than the preset height. Specifically, since the items to be detected are different, the amount of sample used and the total amount of reagent to be added are also different, which results in that the liquid level in the cuvette is not uniform when the cuvette is put into the magnetic separation plate, and the set height of the magnet in the magnetic separation mechanism for performing the magnetic separation later is fixed. For example, referring to fig. 3, when the liquid level of the reaction cup 100 is L1 and the liquid level Gao Duyuan is far lower than the height of the magnetic separation mechanism 200, the magnetic separation mechanism 200 adsorbs the magnetic beads 300 in the reaction cup 100, and the magnetic beads 300 are accumulated on the side of the bottom of the reaction cup 100 close to the magnetic separation mechanism 200 due to the liquid amount limitation, which results in poor adsorption effect of the magnetic separation mechanism 200 on the magnetic beads 300.
In this step, the liquid dispensing mechanism is controlled to perform liquid dispensing operation on the reaction cup, so that the liquid level in the reaction cup is greater than a preset height, where the preset height is the height position of the magnetic separation mechanism 200 corresponding to the reaction cup 100, i.e. the L2 position shown in fig. 2.
Referring to fig. 4, fig. 4 is a schematic diagram showing the effect of the magnetic separation mechanism on adsorbing and aggregating magnetic beads after liquid preparation. After the liquid is prepared, the liquid level of the reaction cup 100 is positioned at the L3 position, the magnetic separation mechanism 200 adsorbs and gathers the magnetic beads 300 in the reaction cup 100, the magnetic beads 300 move to the side wall of the reaction cup 100 in the liquid under the adsorption action of the magnetic separation mechanism 200, are uniformly distributed on the side wall of the reaction cup 100, the accumulation phenomenon is obviously reduced, and the magnetic separation effect is improved.
In one embodiment, the preset height of the liquid prepared from the reaction cup in the step is greater than or equal to 1/3 of the height of the magnet in the magnetic separation mechanism corresponding to the next magnetic separation operation.
Specifically, after this step, the reaction cup is subjected to a magnetic separation operation by a magnetic separation mechanism. Referring to fig. 5, fig. 5 is a schematic diagram illustrating a positional relationship between the magnetic separation mechanism and the magnet. The height of the magnet 200 is L, and the preset height of the prepared solution is greater than or equal to 1/3L, that is, the preset height may be 1/3L, 1/2L, etc., and the value of the preset height may be determined according to the height of the magnet 200 and the amount of the magnetic beads, so as to ensure that a certain height exists at the liquid level in the reaction cup 100 after the solution is prepared, so that when the magnet 200 adsorbs the magnetic beads 300, the magnetic beads 300 are not accumulated due to too little liquid in the reaction cup, but can be more uniformly distributed on the inner wall of the reaction cup 100, and the cleaning effect of the magnetic beads is enhanced.
The height L of the magnet is the height of the exposed magnet part opposite to the side wall of the reaction cup, no matter whether the bottom of the magnet is arranged on the same horizontal plane or not at the bottom of the reaction cup.
S30: and controlling the liquid discharging mechanism to perform liquid discharging operation on the reaction cup.
Wherein, the liquid draining operation is to drain the liquid in the reaction cup. The step controls the liquid discharging mechanism to perform liquid discharging operation on the reaction cup, and discharges liquid in the reaction cup and interfering substances in the liquid.
The liquid draining mechanism is generally used for penetrating into the reaction cup by using a long needle, and draining the liquid in the reaction cup by suction.
S40: and controlling the liquid injection mechanism to perform liquid injection operation on the reaction cup.
The liquid injection mechanism generally injects liquid into the reaction cup by using a short needle. The liquid injection operation may be to inject a cleaning liquid into the reaction cup for cleaning in a subsequent cleaning step, or to inject a detection substrate into the reaction cup for use in a subsequent optical detection step.
The detection substrate in this embodiment is a cleaning solution for cleaning the magnetic beads. In other realizable forms, the detection substrate may also be a luminescent substrate (enzymatic chemiluminescence) or an oxidizing agent (direct chemiluminescence).
Wherein, the operation step of S20 is performed before the operation step of S30 is performed for the first time, that is, the liquid preparation step is performed before the liquid discharge operation of the reaction cup is performed by the liquid discharge mechanism under the first control.
Referring to fig. 6, fig. 6 is a schematic block diagram illustrating a flow chart of an embodiment of the liquid dispensing of the reaction cup in step S20 of the present application. The liquid preparation step of the embodiment comprises the following steps:
s201: the current liquid level height of the reaction cup is obtained.
The liquid level of the reaction cup is detected to obtain the current liquid level height of the reaction cup. The optical detection instrument can obliquely emit a detection beam to the liquid level, and the propagation direction of the reflection beam can be changed when the liquid level has the height difference due to the fixed incidence direction of the detection beam, so that the current liquid level height information can be obtained by detecting the reflection beam. The current liquid level height can also be detected by utilizing sound waves, specifically, the sound waves can be sent to the liquid level, and the current liquid level height is calculated by recording the time when the feedback sound waves are received.
Optionally, directly acquiring a detection item corresponding to the reaction liquid in the reaction cup, and determining the current liquid level height of the reaction cup according to the detection item. Specifically, under a specific detection item, a reaction liquid with a specific capacity is injected into the reaction cup, so that the detection item of the current reaction is directly acquired, and the current liquid level height is directly determined according to the detection item. The method can directly acquire the current liquid level height information without a series of calculation to acquire the current liquid level height information, and is convenient, quick and short in time consumption.
S202: judging whether the current liquid level height is larger than a preset height.
If the current liquid level height is not greater than the preset height, the current reaction cup is determined to be required to be matched, and step S203 is executed. Otherwise, if the current liquid level height is larger than the preset height, the liquid preparation operation is not needed.
S203: the liquid distribution mechanism is controlled to perform liquid distribution operation on the reaction cup so that the liquid level in the reaction cup reaches the preset height.
Referring to fig. 7, the steps may specifically include:
s2031: and determining the liquid preparation capacity according to the current liquid level height and the preset liquid level height.
Specifically, the liquid level difference is calculated according to the current liquid level height and the preset liquid level height, and the liquid level difference and the cross-sectional area of the reaction cup are used for calculating to obtain the liquid distribution capacity, wherein the liquid distribution capacity is the amount of the cleaning liquid to be injected into the reaction cup.
S2032: the liquid distribution mechanism is controlled to perform liquid distribution operation on the reaction cup according to the liquid distribution capacity, so that the liquid level height in the reaction cup reaches a preset height.
The step controls the liquid dispensing mechanism to quantitatively absorb the cleaning liquid according to the liquid dispensing capacity, and injects the cleaning liquid into the reaction cup, so that the liquid level of the reaction cup reaches the preset height.
S204: the liquid preparation operation is not carried out.
So far, the liquid preparation operation of the reaction cup can be completed according to the liquid preparation requirement of the reaction cup.
According to the embodiment, the current liquid level height of the reaction cup is detected to judge whether the current liquid level height meets the preset height or not, and then whether the liquid preparation operation is carried out on the reaction cup or not is determined, so that the liquid level height can be matched with the magnet height of the magnetic separation mechanism in the next magnetic separation operation after the reaction cup is detected and subjected to the liquid preparation mechanism, and the cleaning effect of the magnetic beads is better.
The liquid dispensing mechanism and the liquid dispensing mechanism adopt the same liquid dispensing needle to complete liquid dispensing, namely, the liquid dispensing mechanism is controlled to dispense liquid to the reaction cup when liquid dispensing operation is required, and the liquid dispensing mechanism is controlled to perform liquid dispensing operation when cleaning operation is required by quantitatively injecting cleaning liquid. The liquid injection needle of the liquid preparation mechanism can be fixedly arranged, and when the liquid injection needle is fixedly arranged, the liquid injection operation is carried out on the reaction cup before the reaction cup is removed from the magnetic separation disc by the cup grabbing mechanism.
In further embodiments, the dispensing mechanism and the priming mechanism employ the same priming needle to complete priming, and the priming needle is movable between the magnetic separation disc and the optical detection site. That is, the liquid injection needle of the liquid preparation mechanism may be movably disposed between the detection area and the magnetic separation plate, and after the cleaning is completed, the cup grasping mechanism may move the reaction cup from the magnetic separation plate to the optical detection position of the detection area, and the liquid preparation mechanism may be controlled to inject liquid into the reaction cup at the optical detection position, or may be controlled to inject liquid at a position in the transfer path of the reaction cup from the magnetic separation plate to the optical detection position of the detection area. On the other hand, when the liquid is required to be prepared from the reaction cup, the liquid preparation mechanism is controlled to prepare the liquid from the reaction cup at the liquid preparation level.
The application also provides a belt cleaning device of magnetic separation reaction cup, this belt cleaning device includes: the liquid dispensing assembly is used for performing liquid dispensing operation on the reaction cup on the magnetic separation disc; the liquid injection assembly is used for carrying out liquid injection operation on the reaction cup; the liquid draining assembly is used for draining liquid for the reaction cup; the controller is used for being coupled to the liquid preparation component, the liquid injection component and the liquid discharge component respectively, and is used for controlling the liquid preparation component to prepare liquid for the reaction cup, the liquid injection component to inject liquid for the reaction cup, and the liquid discharge component to discharge the liquid in the reaction cup.
Wherein, annotate liquid subassembly and the multiplexing power device of flowing back subassembly timesharing in order to accomplish notes liquid operation and flowing back operation, simplify the device.
Referring to fig. 8, fig. 8 is a schematic structural diagram of an embodiment of a cleaning device for a magnetic separation reaction cup according to the present application, and the connection lines between the components in fig. 8 represent pipelines for transporting liquid.
The liquid preparation assembly comprises: the device comprises a first power assembly SS, a first liquid injection needle D1 and a first controllable valve LV1, wherein the public end of the first controllable valve LV1 is connected with the first power assembly SS, the first end of the first controllable valve LV1 is connected with a cleaning liquid container C1, and the second end of the first controllable valve LV1 is connected with the first liquid injection needle D1.
In this embodiment, the controller is configured to perform the following operations: controlling the first controllable valve LV1 to communicate the cleaning liquid container C1 with the first power assembly SS; controlling the first power assembly SS to generate negative pressure so as to suck the cleaning liquid in the cleaning liquid container C1; the first controllable valve LV1 is controlled to be communicated with the first power component SS and the first liquid injection needle D1; the first power assembly SS is controlled to generate positive pressure so as to push the sucked cleaning liquid out of the first liquid injection needle D1, thereby completing the liquid preparation operation.
Alternatively, the controller may control the amount of the cleaning liquid sucked from the cleaning liquid container C1, specifically, by controlling the pushing distance of the first power assembly SS to the injector, the amount of the cleaning liquid sucked from the cleaning liquid container C1 is controlled, so that the liquid level in the reaction cup is configured to a preset height when the reaction cup needs to be dispensed, and the cleaning effect of the magnetic beads in the reaction cup is improved. The amount of the cleaning liquid required to be sucked from the cleaning liquid container C1 during liquid preparation, namely the liquid preparation amount, is calculated through the current liquid level height in the reaction cup and the preset liquid level height, and is determined according to the current liquid level height in the reaction cup.
In the different operation time sequences, the liquid preparation component can complete different operations, one is that when liquid preparation is to be carried out on the reaction cup, the liquid preparation component injects the cleaning liquid amount required to prepare the liquid into the reaction cup according to the requirement, and the other is that when quantitative cleaning liquid is required to be injected into the reaction cup for cleaning, the first power component SS is controlled to push the injector for a certain preset distance so as to control the injector to absorb the cleaning liquid with a preset capacity from the cleaning liquid container C1.
When the reaction cup is required to be injected with a quantitative cleaning solution for cleaning, the step is not the liquid preparation step at present, but the step is different from the step of injecting the cleaning solution in that the reaction cup is provided with a reaction solution for incubation during the liquid preparation step, whether the liquid preparation is carried out or not and the amount of the liquid preparation are required to be determined according to the amount of the reaction solution, and the cleaning solution is quantitatively injected during the liquid injection step.
Wherein, annotate liquid subassembly and include: the second power assembly SM, the second annotate liquid needle D2 and the controllable valve LV2, wherein, the public end of the controllable valve LV2 is connected the second power assembly SM, and the washing liquid container C1 is connected to the first end of the controllable valve LV2, and the second annotate liquid needle D2 is connected to the second end of the controllable valve LV 2.
In this embodiment, the controller is configured to perform the following operations: controlling the second controllable valve LV2 to communicate the cleaning liquid container C1 with the second power assembly SM; controlling the second power assembly SM to generate negative pressure so as to suck the cleaning liquid in the cleaning liquid container C1; controlling the second controllable valve LV2 to communicate the second power assembly SM and the second liquid injection needle D2; the second power assembly SM is controlled to generate positive pressure so as to push the sucked cleaning liquid out of the second liquid injection needle D2, and thus the liquid injection operation is completed.
Wherein, flowing back subassembly includes: the device comprises a first liquid discharge needle M1, a second liquid discharge needle M2, a third power assembly P1 and a fourth power assembly P3, wherein a first end of the third power assembly P1 is connected with the first liquid discharge needle M1, and a second end of the third power assembly P1 is connected with a waste liquid container C2; the first end of the fourth power component P3 is connected with the second liquid draining needle M2, and the second end of the fourth power component P3 is connected with the waste liquid container C2.
In this embodiment, the controller is configured to perform the following operations: controlling the third power component P1 to generate negative pressure so as to suck the liquid in the reaction cup through the first liquid discharging needle M1; and controlling the fourth power assembly P3 to generate negative pressure so as to suck the liquid in the reaction cup through the second liquid discharging needle M2.
The liquid draining assembly can utilize the third power assembly P1 to generate negative pressure when the first liquid draining needle M1 is required to drain the reaction cup, and the liquid in the reaction cup is sucked through the first liquid draining needle M1 to drain the reaction cup. And when the reaction cup needs to be drained by using the second drainage needle M2, negative pressure is generated by using the fourth power component P3, and the liquid in the reaction cup is sucked by the second drainage needle M2 to drain the reaction cup.
Wherein, the flowing back subassembly still includes: the cleaning device comprises a third controllable valve LV3, a first cleaning swab W1, a fifth power assembly P2, a fourth controllable valve LV4, a second cleaning swab W2 and a sixth power assembly P4, wherein the public end of the third controllable valve LV3 is connected with the second power assembly SM, and the first end of the third controllable valve LV3 is connected with a cleaning liquid container C1; the liquid inlet of the first cleaning swab W1 is connected with the second end of the third controllable valve LV 3; the first end of the fifth power component P2 is connected with the liquid outlet of the first cleaning swab W1, and the second end of the fifth power component P2 is connected with the waste liquid container C2; the public end of the fourth controllable valve LV4 is connected with the second power assembly SM, and the first end of the fourth controllable valve LV4 is connected with the cleaning liquid container C1; the liquid inlet of the second cleaning swab W2 is connected with the second end of the fourth controllable valve LV 4; the first end of the sixth power component P4 is connected with the liquid outlet of the second cleaning swab W2, and the second end of the sixth power component P4 is connected with the waste liquid container C2.
In this embodiment, the controller is configured to perform the following operations: controlling the third controllable valve LV3 to be communicated with the cleaning liquid container C1 and the second power assembly SM, controlling the fourth controllable valve LV4 to be communicated with the cleaning liquid container C1 and the second power assembly SM, and controlling the second power assembly SM to generate negative pressure so as to absorb the cleaning liquid in the cleaning liquid container C1; controlling the third controllable valve LV3 to communicate with the second power assembly SM and the first cleaning swab W1, and controlling the fourth controllable valve LV4 to communicate with the second power assembly SM and the second cleaning swab W2; controlling the second power assembly SM to generate positive pressure so as to push the sucked cleaning liquid out of the second power assembly SM to the first cleaning swab W1 and the second cleaning swab W2; and controlling the fifth power assembly P2 to generate negative pressure to suck the liquid in the first cleaning swab W1 to the waste liquid container C2, and controlling the sixth power assembly P4 to generate negative pressure to suck the liquid in the second cleaning swab W2 to the waste liquid container C2.
The liquid draining assembly can utilize the third controllable valve LV3 to be communicated with the cleaning liquid container C1 and the second power assembly SM after the reaction cup is drained by using the first liquid draining needle M1, negative pressure is generated by the second power assembly SM to absorb the cleaning liquid in the cleaning liquid container C1, then, positive pressure is generated by the third controllable valve LV3 to be communicated with the second power assembly SM and the first cleaning swab W1 by the second power assembly SM, the cleaning liquid in the cleaning liquid container C1 is absorbed, the absorbed cleaning liquid is pushed out from the second power assembly SM to the first cleaning swab W1, negative pressure is generated by the fifth power assembly P2, and the liquid in the first cleaning swab W1 is absorbed to the waste liquid container C2, so that the first liquid draining needle M1 is cleaned.
Similarly, after the reaction cup is drained by using the first drain needle M2, the fourth controllable valve LV4 may be used to communicate the cleaning solution container C1 with the second power assembly SM, and negative pressure is generated by the second power assembly SM to suck the cleaning solution in the cleaning solution container C1, and then, the fourth controllable valve LV4 is used to communicate the second power assembly SM with the second cleaning swab W2, so that the sucked cleaning solution is pushed out from the second power assembly SM to the second cleaning swab W2, and negative pressure is generated by the sixth power assembly P4 to suck the liquid in the second cleaning swab W2 to the waste solution container C2, so as to clean the second drain needle M2.
In this embodiment, the first cleaning swab W1 is used to perform the cleaning operation on the first liquid discharge needle M1, the second cleaning swab W2 is used to perform the cleaning operation on the second liquid discharge needle M2, and the same power device is reused in different operation time sequences with the injection operation performed by the first liquid injection needle D1 and the injection operation performed by the second liquid injection needle D2, so that the device is simplified. Further, the liquid preparation assembly is utilized to prepare liquid for the reaction cup, so that the recovery rate of the magnetic beads is improved, and the cleaning effect of the magnetic beads is enhanced. Furthermore, the first liquid injection needle D1 can be operated again to perform liquid preparation and quantitative cleaning liquid injection, and the device is further simplified due to multiple purposes of one needle.
In the above embodiment, the device needs to be provided with two injection needles, and in another embodiment, referring to fig. 9, the cleaning device further includes: the third liquid injection needle D3 and the fifth controllable valve LV5, wherein the public end of the fifth controllable valve LV5 is connected with the second end of the fourth controllable valve LV4, the first end of the fifth controllable valve LV5 is connected with the third liquid injection needle D3, and the second end of the fifth controllable valve LV5 is connected with the liquid inlet of the second cleaning swab W2.
In this embodiment, the controller is further configured to: controlling the fifth controllable valve LV5 and the fourth controllable valve LV4 to be communicated with the second power assembly SM and the cleaning liquid container C1; controlling the second power assembly SM to generate negative pressure so as to suck the cleaning liquid in the cleaning liquid container C1; controlling the fifth controllable valve LV5 and the fourth controllable valve LV4 to be communicated with the second power assembly SM and the third liquid injection needle D3; the second power assembly SM is controlled to generate positive pressure so as to push the sucked cleaning liquid out of the third liquid injection needle D3, and the liquid injection operation is completed.
The controller is also configured to: controlling the fourth controllable valve LV4 to communicate the cleaning liquid container C1 with the second power assembly SM; controlling the second power assembly SM to generate negative pressure so as to suck the cleaning liquid in the cleaning liquid container C1; controlling the fourth controllable valve LV4 and the fifth controllable valve LV5 to communicate the second power assembly SM and the second cleaning swab W2, and controlling the second power assembly SM to generate positive pressure so as to push the sucked cleaning fluid from the second power assembly SM to the second cleaning swab W2; the sixth power assembly P4 is controlled to generate negative pressure to suck the liquid in the second washing swab W2 to the waste liquid container C2, thereby washing the first drain needle M1.
In this embodiment, when the reaction cup is cleaned, the operation of each component is controlled according to the cleaning time sequence of the reaction cup, and the cleaning of the reaction cup is completed in cooperation.
Referring to fig. 10, fig. 10 is a schematic diagram illustrating an exemplary sample analyzer according to the present application.
The sample analysis device 1000 comprises a magnetic separation disc 1001 and a cleaning device 1002, wherein a plurality of stations are arranged on the magnetic separation disc 1001 and are used for placing reaction cups; the cleaning device 1002 is arranged corresponding to the working position on the magnetic separation disk, and the cleaning device 1002 is the cleaning device for the magnetic separation reaction cup provided in each embodiment. The sample analyzer 1000 may be a chemo-optical immunoassay analyzer or the like.
In an embodiment, referring to fig. 11, the total number of stations of the magnetic separation disc of this embodiment is 11, each station is correspondingly provided with an operating mechanism, and the operating mechanisms sequentially include: a cup grabbing mechanism 501, a liquid preparation mechanism 502, a first magnetic separation mechanism 503, a second magnetic separation mechanism 504, a third magnetic separation mechanism 505, a first liquid discharge mechanism 506, a fourth magnetic separation mechanism 507, a second liquid discharge mechanism 508, a first liquid injection mechanism 509, a fifth magnetic separation mechanism 510, and a second liquid injection mechanism 511. The controller is also used for controlling each operating mechanism.
Wherein, the liquid dispensing mechanism 502 corresponds to the first liquid injection needle D1, the first liquid discharging mechanism 506 corresponds to the first liquid discharging needle M1, the second liquid discharging mechanism 508 corresponds to the second liquid discharging needle M2, the first liquid injection mechanism 509 corresponds to the second liquid injection needle D2, and the second liquid injection mechanism 511 corresponds to the third liquid injection needle D3.
In the cleaning device of this embodiment, the magnetic separation disc is controlled to rotate three stations each time, and the rotation direction is controlled to be counterclockwise.
Wherein, when the magnetic separation disk rotates first circle, the transfer path of reaction cup is: cup grasping mechanism 501-second magnetic separation mechanism 504-fourth magnetic separation mechanism 507-fifth magnetic separation mechanism 510; when rotating the second circle, the transfer path of the reaction cup is: liquid preparation mechanism 502-third magnetic separation mechanism 505-second liquid discharge mechanism 508-second liquid injection mechanism 511; and when the reaction cup rotates for the third circle, the transfer path of the reaction cup is as follows: first magnetic separation mechanism 503-first drain mechanism 506-first fill mechanism 509-cup grasping mechanism 501.
For a reaction cup to be cleaned, the cleaning flow is as follows:
in a first operating cycle, the cuvette handling mechanism 501 is controlled to transfer cuvettes to the magnetic separation discs.
Subsequently, in a second operation cycle, the magnetic separation disk is controlled to rotate three stations counterclockwise, the reaction cup is transferred to the second magnetic separation mechanism 504, and the magnetic beads in the reaction cup are adsorbed by the adsorption of the second magnetic separation mechanism 504 and gathered to an end close to the second magnetic separation mechanism 504.
In the third operation period, the magnetic separation disc is controlled to rotate three stations anticlockwise, the reaction cup is transported to the fourth magnetic separation mechanism 507, and the magnetic beads in the reaction cup are adsorbed by the adsorption action of the fourth magnetic separation mechanism 507 and gathered to one end close to the fourth magnetic separation mechanism 507.
In the fourth operation period, the magnetic separation disc is controlled to rotate three stations anticlockwise, the reaction cup is transported to the fifth magnetic separation mechanism 510, and the magnetic beads in the reaction cup are adsorbed by the adsorption action of the fifth magnetic separation mechanism 510 and gathered to the end close to the fifth magnetic separation mechanism 510.
In a fifth operation period, the magnetic separation disc is controlled to rotate anticlockwise for three stations, the reaction cup is transferred to the liquid distribution mechanism 502, the liquid distribution mechanism 502 is controlled to add cleaning liquid to the reaction cup according to requirements, so that liquid in the reaction cup reaches a preset capacity, the cleaning effect of the magnetic beads is enhanced, and if the liquid in the reaction cup reaches the preset capacity, the liquid distribution mechanism 502 is controlled not to perform any operation. Wherein, the liquid preparation is carried out by the following steps: controlling the first controllable valve LV1 to communicate the cleaning liquid container C1 with the first power assembly SS; controlling the first power assembly SS to generate negative pressure so as to suck the cleaning liquid in the cleaning liquid container C1; the first controllable valve LV1 is controlled to be communicated with the first power component SS and the first liquid injection needle D1; the first power assembly SS is controlled to generate positive pressure so as to push the sucked cleaning liquid out of the first liquid injection needle D1, thereby completing the liquid preparation operation.
In the sixth operation period, the magnetic separation disk is controlled to rotate three stations anticlockwise, the reaction cup is transported to the third magnetic separation mechanism 505, and the magnetic beads in the reaction cup are adsorbed by the adsorption action of the third magnetic separation mechanism 505 and gathered to the end close to the third magnetic separation mechanism 505.
In a seventh operation period, the magnetic separation disc is controlled to rotate three stations anticlockwise, the reaction cup is transferred to the second liquid discharging mechanism 508, and the second liquid discharging mechanism 508 is controlled to discharge the cleaning liquid in the reaction cup. Wherein, drain is carried out by the following steps: the second liquid discharging needle M2 is controlled to move downwards, the fourth power component P3 is controlled to generate negative pressure so as to absorb liquid in the reaction cup through the second liquid discharging needle M2, and the second liquid discharging needle M2 is controlled to move upwards, so that the lower end part of the second liquid discharging needle M2 does not influence the transfer operation of the reaction cup.
Then, the fourth controllable valve LV4 is controlled to be communicated with the cleaning liquid container C1 and the second power assembly SM, the second power assembly SM is controlled to generate negative pressure so as to suck the cleaning liquid in the cleaning liquid container C1, the fourth controllable valve LV4 and the fifth controllable valve LV5 are controlled to be communicated with the second power assembly SM and the second cleaning swab W2, the second power assembly SM is controlled to generate positive pressure so as to push the sucked cleaning liquid from the second power assembly SM to the second cleaning swab W2, the sixth power assembly P4 is controlled to generate negative pressure so as to suck the liquid in the second cleaning swab W2 to the waste liquid container C2, and the cleaning operation of the second liquid discharging needle M2 is completed
In the eighth operation period, the magnetic separation disc is controlled to rotate three stations anticlockwise, the reaction cup is transferred to the second liquid injection mechanism 511, and the second liquid injection mechanism 511 is controlled to add cleaning liquid to the reaction cup so as to clean the magnetic beads. The liquid injection operation is carried out through the following steps: controlling the fifth controllable valve LV5 and the fourth controllable valve LV4 to be communicated with the second power assembly SM and the cleaning liquid container C1; controlling the second power assembly SM to generate negative pressure so as to suck the cleaning liquid in the cleaning liquid container C1; controlling the fifth controllable valve LV5 and the fourth controllable valve LV4 to be communicated with the second power assembly SM and the third liquid injection needle D3; the second power assembly SM is controlled to generate positive pressure so as to push the sucked cleaning liquid out of the third liquid injection needle D3, and the liquid injection operation is completed.
In the ninth operation period, the magnetic separation disk is controlled to rotate three stations counterclockwise, the reaction cup is transported to the first magnetic separation mechanism 503, and the magnetic beads in the reaction cup are adsorbed by the adsorption action of the first magnetic separation mechanism 503 and gathered to the end close to the first magnetic separation mechanism 503.
In the tenth operation period, the magnetic separation disc is controlled to rotate three stations anticlockwise, the reaction cup is transferred to the first liquid draining mechanism 506, and the first liquid draining mechanism 506 is controlled to drain the cleaning liquid in the reaction cup. Wherein, liquid discharge is carried out through the following steps: the first liquid discharging needle M1 is controlled to move downwards, the third power component P1 is controlled to generate negative pressure so as to absorb liquid in the reaction cup through the first liquid discharging needle M1, and the first liquid discharging needle M1 is controlled to move upwards, so that the lower end part of the first liquid discharging needle M1 does not influence the transfer operation of the reaction cup.
Then, the third controllable valve LV3 is controlled to communicate the cleaning solution container C1 with the second power assembly SM, the second power assembly SM is controlled to generate negative pressure to suck the cleaning solution in the cleaning solution container C1, the third controllable valve LV3 is controlled to communicate the second power assembly SM with the first cleaning swab W1, the second power assembly SM is controlled to generate positive pressure to push the sucked cleaning solution out of the second power assembly SM to the first cleaning swab W1, the fifth power assembly P2 is controlled to generate negative pressure to suck the liquid in the first cleaning swab W1 to the waste liquid container C2, and the cleaning operation of the first liquid discharge needle M1 is completed.
In the eleventh operation cycle, the magnetic separation plate is controlled to rotate three stations counterclockwise, the reaction cup is transferred to the first liquid injection mechanism 509, and the first liquid injection mechanism 509 is controlled to add a detection substrate to the reaction cup. The liquid injection operation is carried out by the following steps: controlling the second controllable valve LV2 to communicate the cleaning liquid container C1 with the second power assembly SM; controlling the second power assembly SM to generate negative pressure so as to suck the cleaning liquid in the cleaning liquid container C1; controlling the second controllable valve LV2 to communicate the second power assembly SM and the second liquid injection needle D2; the second power assembly SM is controlled to generate positive pressure so as to push the sucked cleaning liquid out of the second liquid injection needle D2, and thus the liquid injection operation is completed.
In the twelfth operation period, the magnetic separation disc is controlled to rotate three stations anticlockwise, the reaction cup is transferred to the cup grabbing mechanism 501, and the cup grabbing mechanism 501 is controlled to take out the reaction cup, and the reaction cup can be taken out and placed in an optical detection area for optical detection. Thus, the first-order cleaning of the reaction cup is completed, and if more-order cleaning is required, the number of rotation cycles of the magnetic separation disk can be increased. Each operating mechanism is controlled in a time sharing way so as to finish multi-stage cleaning. The liquid preparing mechanism can perform liquid preparing operation and quantitative injection cleaning operation, and the implementation mode is not repeated here.
The operation cycle in the above embodiment is a description of the operation angle of the reaction cup, and in practice, the control manner of each operation mechanism is not limited by the operation cycle, for example, the cleaning operation of the first drain needle M1 and the first drain needle M2 is performed after the drain is completed, and is not affected by any period or time division from the first operation cycle to the twelfth operation cycle.
Referring to fig. 12, fig. 12 is a schematic block diagram illustrating an exemplary sample analyzer according to the present application.
The sample analysis device 11 comprises a controller 111 and a memory 112, the memory 112 having stored therein program data which, when executed by the controller 111, implements the steps of the various embodiments of the washing method as provided above.
For the description of each step of the processing execution, please refer to the description of each step of the cleaning method embodiment of the magnetic separation reaction cup in the present application, and the description is omitted herein.
In the embodiments of the present application, the disclosed method for cleaning a magnetic separation cuvette and sample analysis device may be implemented in other ways. For example, the various embodiments of the sample analysis device described above are merely illustrative, e.g., the division of the modules or units is merely a logical functional division, and there may be additional divisions in actual implementation, e.g., multiple units or components may be combined or integrated into another system, or some features may be omitted, or not performed. Alternatively, the coupling or direct coupling or communication connection shown or discussed with each other may be an indirect coupling or communication connection via some interfaces, devices or units, which may be in electrical, mechanical or other form.
The units described as separate units may or may not be physically separate, and units shown as units may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the units may be selected according to actual needs to achieve the purpose of the embodiment.
In addition, each functional unit in each embodiment of the present application may be integrated in one processing unit, or each unit may exist alone physically, or two or more units may be integrated in one unit. The integrated units may be implemented in hardware or in software functional units.
The integrated units, if implemented in the form of software functional units and sold or used as stand-alone products, may be stored in a computer readable storage medium. Based on such understanding, the technical solution of the present application may be embodied essentially or partly contributing to the prior art or in whole or in part in the form of a software product, which is stored in a storage medium.
Referring to fig. 13, fig. 13 is a schematic block diagram illustrating a circuit structure of an embodiment of a computer readable storage medium of the present application, where the computer readable storage medium 1000 stores a computer program 1001, and the computer program 1001 implements steps of the embodiments of the method for cleaning a magnetic separation cuvette of the present application when executed by a controller.
The computer storage medium 1000 may be a usb disk, a removable hard disk, a Read-Only Memory (ROM), a random access Memory (RAM, random Access Memory), a magnetic disk, or an optical disk, etc. various media capable of storing program codes.
The foregoing description is only exemplary embodiments of the present application and is not intended to limit the scope of the present application, and all equivalent structures or equivalent processes using the descriptions and the drawings of the present application, or direct or indirect application in other related technical fields are included in the scope of the present application.
Claims (9)
1. A method for cleaning a magnetic separation cuvette, the method comprising:
obtaining a reaction cup to be cleaned; wherein, the reaction cup is internally provided with a reaction liquid;
controlling the liquid preparation mechanism to perform liquid preparation operation on the reaction cup so that the liquid level height in the reaction cup reaches a preset height, comprising the following steps: acquiring detection items corresponding to the reaction liquid in the reaction cup; determining the current liquid level height of the reaction cup according to the detection item; judging whether the current liquid level height is larger than the preset height; if not, controlling the liquid distribution mechanism to perform liquid distribution operation on the reaction cup so as to enable the liquid level height in the reaction cup to reach the preset height;
controlling a liquid discharge mechanism to perform liquid discharge operation on the reaction cup;
and controlling the liquid injection mechanism to perform liquid injection operation on the reaction cup.
2. The method of claim 1, wherein the step of determining the position of the substrate comprises,
After the liquid is prepared from the reaction cup, the magnetic separation mechanism is utilized to perform magnetic separation operation on the reaction cup, and the preset height is greater than or equal to 1/3 of the height of a magnet in the magnetic separation mechanism.
3. The method of claim 1, wherein the step of determining the position of the substrate comprises,
the step of controlling the liquid dispensing mechanism to perform liquid dispensing operation on the reaction cup is performed before the step of controlling the liquid discharging mechanism to perform liquid discharging operation on the reaction cup for the first time.
4. The method of claim 1, wherein the step of determining the position of the substrate comprises,
the step of obtaining the current liquid level height of the reaction cup comprises the following steps:
and detecting the liquid level of the reaction cup to obtain the current liquid level height of the reaction cup.
5. The method of claim 1, wherein the step of determining the position of the substrate comprises,
the control liquid preparing mechanism performs liquid preparing operation on the reaction cup so that the liquid level height in the reaction cup reaches a preset height, and the control liquid preparing mechanism comprises the following steps:
determining liquid preparation capacity according to the current liquid level height and the preset height;
and controlling the liquid distribution mechanism to perform liquid distribution operation on the reaction cup according to the liquid distribution capacity so as to enable the liquid level height in the reaction cup to reach a preset height.
6. The method of claim 1, wherein the step of determining the position of the substrate comprises,
The control liquid preparation mechanism performs liquid preparation operation on the reaction cup, and the control liquid preparation mechanism comprises:
placing the reaction cup on a magnetic separation disc;
controlling the magnetic separation disc to rotate so as to drive the reaction cup to a liquid level;
controlling the liquid preparation mechanism to perform liquid preparation operation on the reaction cup;
the control liquid injection mechanism performs liquid injection operation on the reaction cup, and comprises the following steps:
controlling the magnetic separation disc to rotate so as to drive the reaction cup to the liquid injection level;
controlling the liquid injection mechanism to perform liquid injection operation on the reaction cup;
the liquid injection mechanism is connected with the liquid injection needle, and the liquid injection mechanism is connected with the liquid injection needle.
7. The method of claim 1, wherein the step of determining the position of the substrate comprises,
the control liquid preparation mechanism performs liquid preparation operation on the reaction cup, and the control liquid preparation mechanism comprises:
placing the reaction cup on a magnetic separation disc;
controlling the magnetic separation disc to rotate so as to drive the reaction cup to a liquid level;
controlling the liquid preparation mechanism to perform liquid preparation operation on the reaction cup;
the control liquid injection mechanism performs liquid injection operation on the reaction cup, and comprises the following steps:
moving the cuvette from the magnetic separation disc to an optical detection position;
controlling the liquid injection mechanism to perform liquid injection operation on the reaction cup;
The liquid preparation mechanism and the liquid injection mechanism adopt the same liquid injection needle to complete liquid injection, and the liquid injection needle can move between the magnetic separation disc and the optical detection position.
8. A sample analysis device comprising a controller and a memory, the memory having stored therein program data which, when executed by the controller, implements the method of any of claims 1-7.
9. A computer readable storage medium, characterized in that the computer readable storage medium has stored therein program data, which when executed by a controller, is adapted to carry out the method according to any of claims 1-7.
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| CN107377563B (en) * | 2017-08-24 | 2021-01-08 | 深圳市亚辉龙生物科技股份有限公司 | Magnetic separation cleaning device and working method thereof and liquid injection needle tube |
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| CN110125076A (en) * | 2018-02-08 | 2019-08-16 | 成都深迈瑞医疗电子技术研究院有限公司 | Magnetic bead cleans separator, magnetic bead cleaning separation method and chemical illumination immunity analysis instrument |
| CN110883039B (en) * | 2018-09-11 | 2021-06-04 | 深圳市新产业生物医学工程股份有限公司 | Adsorption mechanism, cleaning device, chemiluminescence detector and cleaning method |
| CN110823669A (en) * | 2019-11-08 | 2020-02-21 | 广州科方生物技术股份有限公司 | Magnetic separation cleaning method |
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