CN114617069B - Functional bio-based cat litter capable of detecting diseases of cat urinary system - Google Patents
Functional bio-based cat litter capable of detecting diseases of cat urinary system Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/015—Floor coverings, e.g. bedding-down sheets ; Stable floors
- A01K1/0152—Litter
- A01K1/0155—Litter comprising organic material
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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Abstract
The invention discloses a functional bio-based cat litter capable of detecting diseases of a cat urinary system. The cat litter is a particle with a core-shell structure, and the particle consists of a core layer and a shell layer coated on the outer side of the core layer. The core layer consists of 0.1-10 parts by weight of water-absorbing resin and 10-50 parts by weight of baking soda, and the shell layer consists of 10-30 parts by weight of aldehyde group-containing nano-cellulose, 10-80 parts by weight of starch and 0.1-1 part by weight of color developing agent; the aldehyde substitution degree of the nano-cellulose is 0.4-1.0. According to the functional bio-based cat litter, the aldehyde group-containing nano-cellulose is innovatively adopted, and the core-shell structure and the spraying preparation process of cat litter particles are combined, so that the color development sensitivity and durability of the cat litter are greatly improved, the dosage of a color developing agent is reduced, and the production cost is reduced; meanwhile, the color developing device is convenient to use, whether color is developed or not can be distinguished by naked eyes at normal temperature, the problem that home self-inspection is inconvenient is solved, and market demands are met.
Description
Technical Field
The invention belongs to the field of pet products, relates to cat litter, and particularly relates to functional bio-based cat litter particles capable of detecting diseases of a cat urinary system.
Background
At present, the household pets "wang star man" and "meow star man" are gradually becoming the spiritual consolation and the partner in life, and are also important members in many families, which directly causes the blowout type growth of the pet products industry. In 2020, the market scale of the pet industry in China is close to 3000 million yuan, and is estimated to reach 4500 million yuan by 2023 (wealth era 2021 (08): 34-36). Cat litter is an important component of pet products as a product for cats to bury feces and urine, and is sold in quantities listed as top animals in pet products. With the increasing income and living standard of people, the raising owners who are called "shit spades officials" are concerned more and more about the high efficiency and safety of the cat litter, and also begin to pay more attention to the functionality of the cat litter, such as deodorization, comfort and the like. Therefore, on the basis of considering both high efficiency and safety performance, the continuous development of the functional cat litter with high added value is the market demand, is beneficial to the expansion of the pet product market, and has wide market application prospect and great economic value.
Urinary system disease is a common cat disease. After the cat suffers from the disease, symptoms such as frequent hematuria, dysuria, pain and the like can appear. If not treated in a timely manner, the life of the cat may be compromised (modern veterinary medicine 2021 (02): 62-64). Therefore, if the cat litter directly contacting urine can be used for detection, early detection and early treatment can be achieved. To address this problem, various documents have been published which disclose litter that utilizes urine to monitor health of a cat. The invention patent application 202011605177.6 discloses a preparation method of functional color-developing health indication plant deodorization cat litter, which is used for detecting the pH value of cat urine and early warning the health condition of pets. The invention patent application 201811247499.0 discloses a cat litter with a pet urine protein early warning function and a preparation method thereof, and the health condition of a pet is preliminarily reflected by detecting urine protein. The invention patent application 202011564784.2 discloses a cat litter for detecting cat urinary tract diseases and a preparation method thereof, and whether occult blood exists in cat urine is detected by judging whether cat litter particles added with a luminol reagent develop color or not, so that whether the cat has related diseases of urinary system infection or not is presumed. However, the luminol reagent needs to be in a dark room to observe fluorescence, which brings inconvenience to the use of ordinary families. Moreover, since the method prepares the cat litter by blending the coloring agent with other components, two problems are inevitably caused: (1) When the usage amount is high, the cost of the cat litter is increased due to the expensive color reagent; (2) When the using amount is low, the color reaction time is long, the color development is not obvious, and even the detection result is inaccurate due to the false negative phenomenon.
Disclosure of Invention
Aiming at the current demand situation of functional cat litter in the prior art, the invention provides functional bio-based cat litter capable of detecting diseases of cat urinary systems and a preparation method thereof. According to the functional bio-based cat litter, the aldehyde group-containing nano-cellulose is innovatively adopted, and the core-shell structure and the spraying preparation process of cat litter particles are combined, so that the color development sensitivity and durability of the cat litter are greatly improved, the dosage of a color developing agent is reduced, and the production cost is reduced; meanwhile, the color developing device is convenient to use, whether color is developed or not can be distinguished by naked eyes at normal temperature, the problem that home self-inspection is inconvenient is solved, and market demands are met.
The technical scheme of the invention is as follows:
the bio-based cat litter capable of detecting the cat urinary system diseases is a particle with a core-shell structure, and the particle consists of a core layer and a shell layer coated on the outer side of the core layer. The core layer consists of 0.1-10 parts by weight of water-absorbing resin and 10-50 parts by weight of baking soda, and the shell layer consists of 10-30 parts by weight of aldehyde group-containing nano cellulose, 10-80 parts by weight of starch and 0.1-1 part by weight of color developing agent; the aldehyde substitution degree of the nano-cellulose is 0.4-1.0. The aldehyde substitution degree and the dosage of the nano-cellulose are directly related to the color development effect, if the aldehyde substitution degree or the dosage is lower than the range, rapid color development cannot be realized or the color development is not obvious, and if the aldehyde substitution degree or the dosage is higher than the range, the cost of the cat litter can be increased, and the economical efficiency of the cat litter is influenced. The application the bio-based cat litter is after the cat urinates, if the color-developing agent of the shell layer detects that there is occult blood in the urine, the color of the cat litter particle can change, so that the cat can be speculated to infect urinary system diseases, and the cat can seek medical advice as early as possible. Meanwhile, the water-absorbent resin of the cat litter particle nuclear layer can quickly absorb urine, so that convenience is provided for subsequent cleaning of cat litter.
Wherein the water-absorbing resin is one or more of polyvinyl alcohol (PVA), polyacrylic acid (PAA), polyacrylamide (PAM), sodium polyacrylate and potassium polyacrylate. The color developing agent is a benzidine composite reagent, a leuco gentian violet composite reagent, a colorless malachite green composite reagent, an amino black 10B composite reagent or a dihydrofluorescein composite reagent. The color developing agent adopted by the invention can distinguish whether the color changes or not by naked eyes under the environment of room temperature and normal light, thereby greatly improving the practicability of the cat litter for detecting urinary tract diseases in the process of household self-inspection.
The aldehyde group-containing nano-cellulose is prepared by hydrolyzing a cellulose raw material pretreated by molten salt with formic acid; the method comprises the following specific steps: (1) molten salt pretreatment: adding a proper amount of dissolved pulp into 40-60wt% molten salt hydrate solution according to the pulp concentration of 1-20 wt%, soaking at 20-40 deg.C for 5-30min, and cleaning with water until no molten salt component exists to obtain the final productThe treated slurry; the molten salt hydrate is LiBr or ZnCl 2 、AlCl 3 And one or more of lithium perchlorate. (2) hydrolysis of formic acid: adding a proper amount of pretreated slurry into 10-40 wt% aqueous solution of formic acid according to the concentration of the slurry being 1-20 wt%, reacting for 3-8h at 20-40 ℃, and cleaning to obtain the aldehyde group-containing nanocellulose. The aldehyde group-containing nanocellulose is innovatively adopted as the color developing agent carrier, and compared with other bio-based carriers (such as microcrystalline cellulose and starch), the color developing sensitivity (the color developing time is less than 3 seconds) and the color developing durability (the color developing time is more than 15 days) of the cat litter are greatly improved. This is because, under the wet condition, aldehyde groups in the nanocellulose can be chelated with metal ions in blood, thereby significantly improving the sensitivity and durability of color development.
The preparation method of the bio-based cat litter capable of detecting the diseases of the cat urinary system comprises the following steps:
(1) Preparing a core layer: weighing the water-absorbent resin and the baking soda according to the parts by weight, uniformly mixing the water-absorbent resin and the baking soda, and granulating by using a cat litter granulator to obtain a core layer.
(2) Coating a shell layer: weighing 10-30 parts by weight of aldehyde group-containing nanocellulose, 10-80 parts by weight of starch and 0.1-1 part by weight of color developing agent; firstly, spraying starch on the surface of a nuclear layer, then spraying nano-cellulose on the surface of the starch layer, finally uniformly spraying a color-developing agent on the surface of the nano-cellulose layer, and drying to obtain the bio-based cat litter particles.
The invention also provides a color comparison card matched with the bio-based cat litter for use. The color comparison card is a color ribbon strip which is transversely arranged, and the color of the color ribbon strip is arranged from left to right from light to dark. The color card comprises five areas from 291C to 295C, and the detection concentrations are 0.001%,0.005%,0.01%,0.05% and more than 0.1%. Through comparing with the colorimetric card, whether occult blood exists in the urine of the cat can be accurately detected, the blood concentration in the urine can be further quantitatively known, the health condition of the cat can be more accurately presumed, and the kit has definite guiding significance for the next treatment measures.
The invention has the beneficial effects that:
(1) The shell layer of the cat litter box adopts the nano-cellulose containing aldehyde groups, so that the detection sensitivity of the cat litter particles is high, the lowest blood concentration in the urine of a cat can be detected to be 0.001%, the color development time is less than 3 seconds, the color development durability is more than 15 days, the requirement on the color development cat litter in the market is met, and the application prospect is good.
(2) The cat litter adopts a core-shell structure and a spraying preparation process, so that the preparation method is simple, the use amount of the color developing agent is reduced, and the preparation cost of the cat litter is reduced; and the water-absorbent resin of the core layer is greatly beneficial to ensuring the household sanitation.
(3) The cat litter not only realizes rapid and instant detection of cat urinary system diseases, but also can distinguish whether the cat urinary system diseases discolor or not by naked eyes under the environment of room temperature and normal light, thereby greatly meeting the requirement of family self-checking.
Drawings
FIG. 1 is a schematic structural view of a functional bio-based litter particle described herein;
fig. 2 is a pictorial representation of the litter particles made in example 1.
FIG. 3 is a graphical representation of the absence of color change in cat litter pellets prepared in example 1 when exposed to urine without occult blood.
Fig. 4 is a graph showing the color change of the litter particles made in example 1 when exposed to urine with occult blood (0.001% concentration).
FIG. 5 is a color chart for color comparison in example 1.
FIG. 6 is a graphical representation of the color change of litter pellets in example 1 after exposure to urine with occult blood (0.1% concentration).
Detailed Description
The present invention will be further described with reference to the following examples.
Example 1
The invention provides functional bio-based cat litter particles (figure 1) capable of detecting urinary system diseases, wherein a core layer comprises 10 parts by weight of sodium polyacrylate and 29 parts by weight of sodium bicarbonate, and a shell layer comprises aldehyde nanocellulose (aldehyde substitution degree 1, 10 parts by weight), starch (51 parts by weight) and tetramethylbenzidine (0.2 part by weight). The preparation process comprises the following steps: the cat litter is granulated by a cat litter granulator to obtain cat litter core layer particles, then starch, nano-cellulose and a color developing agent are sequentially sprayed on the core layer particles, and finally the color developing cat litter particles are prepared by air drying.
The preparation method of the nano-cellulose comprises the following steps: (1) The broadleaf wood dissolving pulp is used as a raw material and is soaked in LiBr solution (with the mass concentration of 45%) for 2 hours; the slurry concentration is 1%, the dipping temperature is 40 ℃, and the pretreated slurry is washed for 3 times by using distilled water with 5 times of mass. (2) Adding the slurry into 10% aqueous solution of formic acid, reacting for 8h at 40 ℃, and washing for 3 times with distilled water of 5 times of the mass to obtain the nano-cellulose with the aldehyde substitution degree of 1.
Detection of cat litter particles: the urine from a normal cat was dropped on the surface of the pellet and the pellet color did not change (FIG. 2). However, when urine containing blood (0.001% concentration) was dropped on the surface of the litter particles, the particles became bluish at 2 seconds (FIG. 3), and after 10 minutes, the color was 291C by comparison with a color chart (FIG. 4). Urine containing blood (0.1% concentration) was dropped on the surface of the litter pellets and the pellets instantaneously (less than 1 second) turned dark blue, which was 295C in color after 10 minutes using a color chart (FIG. 5). In addition, the developed cat litter particles can still keep the original color after being observed for 15 days when being placed in the air at room temperature. The cat litter particles described achieved quantitative detection of occult blood in urine.
Comparative example 1:
functional bio-based cat litter particles were prepared by the same method as in example 1, with the aldehyde nanocellulose being replaced with microcrystalline cellulose, and the other raw materials and ratios were kept unchanged. The urine of a normal cat is dripped on the surface of the particles, and the color of the particles is not changed. However, dropping urine containing blood (at a concentration of 0.001%) on the surface of the litter particles, the particles gradually turned bluish after 10 minutes. At the same time, urine containing blood (0.1% concentration) was dropped on the surface of the litter pellets, and the pellets turned dark blue in 7 minutes. Further, the developed litter pellets were allowed to stand in the air at room temperature for 12 hours, and then observed to turn to the original pale yellow. From this, it was found that the litter pellet had a long development time and poor development durability when detecting occult blood in urine. Compared with example 1, it is demonstrated that the use of the aldehyde-based cellulose significantly improves the color development speed and color development durability of the cat litter.
Example 2:
the invention provides a functional bio-based cat litter particle capable of detecting urinary system diseases, wherein a core layer comprises 0.1 part by weight of polyacrylamide and 10 parts by weight of baking soda, and a shell layer comprises aldehyde nanocellulose (with an aldehyde substitution degree of 0.4 and an aldehyde substitution degree of 30 parts by weight), starch (with an aldehyde substitution degree of 58.5 parts by weight) and a cryptochrome gentian violet composite reagent (with an aldehyde substitution degree of 0.1 part by weight and a shell layer by weight). The preparation process comprises the following steps: the cat litter is granulated by a cat litter granulator to obtain cat litter core layer particles, then starch, nano-cellulose and a color developing agent are sequentially sprayed on the core layer, and finally the cat litter is prepared after air drying.
The preparation method of the nano-cellulose comprises the following steps: (1) Using needle-leaved wood dissolving pulp as raw material in LiBr and ZnCl 2 Dipping the mixture in the solution (the molar ratio is 1:1) for 30 minutes; the slurry concentration was 20%, the dipping temperature was 30 ℃, and the pretreated slurry was washed with 5 times by mass of distilled water for 3 times. (2) Adding the slurry into 40% aqueous solution of formic acid, reacting at 30 deg.C for 3h, and washing with 5 times of distilled water for 3 times to obtain nanocellulose with aldehyde substitution degree of 0.4.
Detection of cat litter particles: the urine of the normal cat is dripped on the surface of the particles, and the color of the particles is not changed. However, when urine (0.01% concentration) containing blood was dropped on the surface of the litter pellets, the pellets became bluish-purple in 2 seconds. The developed cat litter particles are placed in the air at room temperature, and the original color can be still maintained after 15 days of observation.
Comparative example 2: by using the same cat litter pellet preparation method as in example 2, the aldehyde nanocellulose dosage was 3%, and the other formulation and preparation method were unchanged. Urine containing blood (0.01% concentration) was dropped on the surface of the litter pellets, which turned bluish-purple after 20 minutes. In comparison with example 2, it can be seen that the amount of aldehyde cellulose is too low, resulting in less sensitivity to the color development of the litter pellets.
Example 3
The invention provides a functional bio-based cat litter particle capable of detecting urinary system diseases, wherein a core layer comprises 5 parts by weight and 50 parts by weight of polyacrylic acid and baking soda respectively, and a shell layer comprises aldehyde nanocellulose (with aldehyde substitution degree of 0.8 and using amount of 25 parts by weight), starch (with 19.1 parts by weight) and amino black 10B (with 0.9 part by weight) which are compounded for use. The preparation process comprises the following steps: the method comprises the steps of granulating by using a cat litter granulator to obtain cat litter core layer particles, spraying starch, nano-cellulose and a color developing agent on the core layer in sequence, and finally air-drying to obtain the color developing cat litter particles.
The preparation method of the nano-cellulose comprises the following steps: (1) Using wheat straw dissolving pulp as raw material in LiBr and AlCl 3 Mixed (molar ratio 2:1) and immersed for 10 minutes; the slurry concentration was 15%, the dipping temperature was 30 ℃, and the pretreated slurry was washed with distilled water 4 times by mass for 3 times. (2) Adding the slurry into 20% concentration formic acid water solution, reacting for 7h at 35 deg.C, washing with 5 times mass of distilled water for 3 times, and getting the nanometer cellulose with aldehyde substitution degree of 0.8.
Detection of cat litter particles: the urine of a normal cat is dripped on the surface of the particles, and the color of the particles is not changed. However, dropping urine (0.1% concentration) containing blood on the surface of the litter pellets, the pellets turned blue-black within 3 seconds. The developed cat litter particles are placed in the air at room temperature, and the original color can be still maintained after 15 days of observation. Comparative example 3: the same cat litter particle preparation method as in example 3 was used, but the aldehyde nanocellulose was prepared without being impregnated with molten salt, and was directly reacted with formic acid aqueous solution (the reaction conditions were the same as in example 3), and the degree of substitution with aldehyde groups of the obtained aldehyde nanocellulose was only 0.15. Urine (0.1% concentration) containing blood was dropped on the surface of the litter particles, and the particles turned blue-black after 30 minutes. As is clear from comparison with example 3, the aldehyde substitution degree of the aldehyde cellulose was too low, and the color development was not sensitive.
Example 4
The invention provides a functional bio-based cat litter particle capable of detecting urinary system diseases, wherein a core layer is composed of polyacrylic acid and baking soda in the amount of 8 parts by weight and 40 parts by weight respectively, and a shell layer is formed by compounding aldehyde nanocellulose (the aldehyde substitution degree is 0.5, and the amount is 20 parts by weight), starch (the amount is 31 parts by weight) and colorless malachite green (the amount is 0.5 part by weight). The preparation process comprises the following steps: the method comprises the steps of granulating by using a cat litter granulator to obtain cat litter core layer particles, spraying starch, nano-cellulose and a color developing agent on the core layer in sequence, and finally air-drying to obtain the color developing cat litter particles.
The preparation method of the nano-cellulose comprises the following steps: (1) Soaking broadleaf wood dissolving pulp serving as a raw material in a lithium perchlorate solution (with the concentration of 60%) for 5 minutes; the slurry concentration was 12%, the dipping temperature was 22 ℃, and the pretreated slurry was washed with 5 times by mass of distilled water for 3 times. (2) Adding the slurry into 15 percent aqueous solution of formic acid, the concentration of the slurry is 5 percent, reacting for 6h at the temperature of 25 ℃, washing with 4 times of distilled water for 3 times to obtain the nano-cellulose with aldehyde substitution degree of 0.5.
Detection of cat litter particles: the urine of a normal cat is dripped on the surface of the particles, and the color of the particles is not changed. However, dropping urine (0.1% concentration) containing blood on the surface of the litter particles, the particles turned emerald green within 2 seconds. The developed cat litter particles are placed in the air at room temperature, and the original color can be still kept after 15 days of observation.
Example 5
The invention provides a functional bio-based cat litter particle capable of detecting urinary system diseases, wherein a core layer comprises 9 parts by weight of polyvinyl alcohol and 35 parts by weight of sodium bicarbonate, and a shell layer comprises aldehyde nanocellulose (with an aldehyde substitution degree of 0.9 and an aldehyde substitution amount of 15 parts by weight), starch (with an aldehyde substitution amount of 40.8 parts by weight) and dihydrofluorescein (with an aldehyde substitution amount of 0.2 part by weight) in a composite manner. The preparation process comprises the following steps: the cat litter is granulated by a cat litter granulator to obtain cat litter core layer particles, then starch, nano-cellulose and a color developing agent are sprayed on the core layer, and finally the color developing cat litter particles are prepared by air drying.
The preparation method of the nano-cellulose comprises the following steps: (1) Soaking broadleaf wood dissolving pulp serving as a raw material in a LiBr solution (the concentration is 43%) for 5 minutes; the slurry concentration was 1.8%, the dipping temperature was 38 ℃, and the pretreated slurry was washed 3 times with 5 times the mass of distilled water. (2) Adding the slurry into 35% concentration formic acid water solution, reacting for 6h at 35 deg.C, washing with 5 times mass of distilled water for 3 times, and getting the nanometer cellulose with aldehyde substitution degree of 0.9.
Detection of cat litter particles: the urine of a normal cat is dripped on the surface of the particles, and the color of the particles is not changed. However, dropping urine (0.1% concentration) containing blood on the surface of the litter particles, the particles turned fluorescent green within 3 seconds. The developed cat litter particles are placed in the air at room temperature, and the original color can be still maintained after 15 days of observation.
Example 6
The invention provides a functional bio-based cat litter particle capable of detecting urinary system diseases, wherein a core layer comprises polyvinyl alcohol and baking soda in the amount of 10 parts by weight and 50 parts by weight respectively, and a shell layer comprises aldehyde nanocellulose (aldehyde substitution degree 1, and the amount is 30 parts by weight), starch (10 parts by weight) and dihydrofluorescein (0.1 part by weight) which are used in a composite manner. The preparation process comprises the following steps: the cat litter is granulated by a cat litter granulator to obtain cat litter core layer particles, then starch, nano-cellulose and a color developing agent are sprayed on the core layer, and finally the color developing cat litter particles are prepared by air drying.
The preparation method of the nano-cellulose comprises the following steps: (1) Using broadleaf wood dissolving pulp as a raw material to be dipped in LiBr solution (the concentration is 45%) for 10 minutes; the slurry concentration was 5%, the dipping temperature was 40 ℃, and the pretreated slurry was washed 3 times with distilled water 5 times the mass. (2) Adding the slurry into 40% concentration formic acid water solution, reacting for 8h at 40 deg.C, washing with 5 times mass of distilled water for 3 times, and getting the nanometer cellulose with aldehyde substitution degree of 1.
Detection of cat litter particles: the urine of a normal cat is dripped on the surface of the particles, and the color of the particles is not changed. However, dropping urine (0.05% concentration) containing blood on the surface of the litter particles, the particles turned fluorescent green within 3 seconds. The developed cat litter particles are placed in the air at room temperature, and the original color can be still maintained after 15 days of observation.
Example 7
The invention provides a functional bio-based cat litter particle capable of detecting urinary system diseases, wherein a core layer comprises polyacrylic acid and baking soda in an amount of 1 part by weight and 10 parts by weight respectively, and a shell layer comprises aldehyde nanocellulose (aldehyde substitution degree 1, 10 parts by weight), starch (78 parts by weight) and colorless malachite green (1 part by weight) in a composite manner. The preparation process comprises the following steps: the method comprises the steps of granulating by using a cat litter granulator to obtain cat litter core layer particles, spraying starch, nano-cellulose and a color developing agent on the core layer in sequence, and finally air-drying to obtain the color developing cat litter particles.
The preparation method of the nano-cellulose comprises the following steps: (1) Soaking broadleaf wood dissolving pulp serving as a raw material in a LiBr solution (with the concentration of 45%) for 10 minutes, wherein the pulp concentration is 5%, the soaking temperature is 40 ℃, and washing the pretreated pulp with distilled water with the mass of 5 times for 3 times; adding the slurry into 40% concentration formic acid water solution, reacting for 8h at 40 deg.C, washing with 5 times mass of distilled water for 3 times, and getting the nanometer cellulose with aldehyde substitution degree of 1.
Detection of cat litter particles: the urine of a normal cat is dripped on the surface of the particles, and the color of the particles is not changed. However, dropping urine containing blood (concentration of 0.09%) on the surface of the litter pellets, the pellets turned emerald green within 2 seconds. The developed cat litter particles are placed in the air at room temperature, and the original color can be still maintained after 15 days of observation.
In conclusion, the functional bio-based cat litter particle disclosed by the application not only improves the detection sensitivity (the minimum concentration is 0.001%, the color development time is less than 3 seconds) to occult blood in urine and improves the color development durability (more than 15 days) by combining formula optimization and a core-shell structure; the preparation method is simple, the dosage of the color developing agent is reduced, and the preparation cost of the cat litter is reduced. In addition, whether the color is changed or not can be distinguished by naked eyes under the room temperature and normal light environment, and the requirement of family self-inspection is met; and the water-absorbing resin of the cat litter core layer is very beneficial to ensuring the family sanitation. Compared with the existing cat litter in the market, the functional bio-based cat litter meets a plurality of requirements, and has wide market prospect and economic value.
Claims (10)
1. Biological base cat litter of detectable cat urinary system disease, its characterized in that: the cat litter is particles with a core-shell structure, and the particles consist of a core layer and a shell layer coated on the outer side of the core layer; the core layer consists of 0.1-10 parts by weight of water-absorbing resin and 10-50 parts by weight of baking soda, and the shell layer consists of 10-30 parts by weight of aldehyde group-containing nano cellulose, 10-80 parts by weight of starch and 0.1-1 part by weight of color developing agent; the aldehyde substitution degree of the nano-cellulose is 0.4-1.0.
2. The bio-based cat litter of claim 1 capable of detecting feline urinary system disease characterized by: the water-absorbing resin is one or more of polyvinyl alcohol, polyacrylic acid, polyacrylamide, sodium polyacrylate and potassium polyacrylate.
3. The bio-based cat litter of claim 1 capable of detecting feline urinary system disease characterized by: the color developing agent is a benzidine composite reagent, a leuco gentian violet composite reagent, a colorless malachite green composite reagent, an amino black 10B composite reagent or a dihydrofluorescein composite reagent.
4. The bio-based cat litter of any one of claims 1-3 capable of detecting feline urinary system disease wherein: the aldehyde group-containing nano-cellulose is prepared by hydrolyzing a cellulose raw material pretreated by molten salt with formic acid.
5. The bio-based cat litter of claim 4 wherein said bio-based cat litter is selected from the group consisting of: the method comprises the following specific steps: (1) molten salt pretreatment: adding a proper amount of the dissolved slurry plate into a molten salt hydrate solution, soaking for 5-30min at 20-40 ℃, and then washing with water until no molten salt component exists to obtain pretreated slurry; (2) hydrolysis of formic acid: adding a proper amount of pretreated slurry into a formic acid aqueous solution, reacting at 20-40 ℃ for 3-8h, and cleaning to obtain the aldehyde group-containing nanocellulose.
6. The detectable cat of claim 5Bio-based cat litter for urinary system disease, comprising: the molten salt hydrate in the step (1) is LiBr or ZnCl 2 、AlCl 3 And one or more of lithium perchlorate, wherein the concentration of the molten salt hydrate solution is 40-60wt%, and the concentration of the formic acid aqueous solution in the step (2) is 10-40 wt%; the pulp concentration of the step (1) and the pulp concentration of the step (2) are both 1-20%.
7. The method of making biobased cat litter according to any one of claims 1-6 comprising: the method comprises the following steps:
(1) Preparing a core layer: weighing water-absorbing resin and baking soda according to parts by weight, uniformly mixing the water-absorbing resin and the baking soda, and granulating by using a cat litter granulator to obtain a core layer;
(2) Coating a shell layer: weighing 10-30 parts by weight of aldehyde group-containing nanocellulose, 10-80 parts by weight of starch and 0.1-1 part by weight of color developing agent; firstly, spraying starch on the surface of a nuclear layer, then spraying nano-cellulose on the surface of the starch layer, finally uniformly spraying a color-developing agent on the surface of the nano-cellulose layer, and drying to obtain the bio-based cat litter particles.
8. The method of making bio-based cat litter of claim 7 wherein said method comprises the steps of: the grain size of the nuclear layer prepared in the step (1) is 0.05 cm-0.8 cm; the particle size of the cat litter particles prepared in step (2) is 0.1 cm-1 cm.
9. A color chart for use with the bio-based litter of any one of claims 1-6 wherein: the color comparison card is a color ribbon strip which is transversely arranged, and the color of the color ribbon strip is arranged from left to right from light to dark.
10. The colorimetric card of claim 9, wherein: the color card comprises five regions from 291C to 295C, and the detection concentrations are 0.001%,0.005%,0.01%,0.05% and more than 0.1% respectively.
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| CN116953258A (en) * | 2023-08-02 | 2023-10-27 | 山东瑞达硅胶有限公司 | Pet urine protein detection particle and preparation method thereof |
| CN118216438A (en) * | 2024-03-29 | 2024-06-21 | 威海龙峰硅胶有限公司 | Preparation method of allochroic silica gel cat litter for renal function detection |
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| CN101140278B (en) * | 2007-11-05 | 2012-01-11 | 刘世安 | Preparation method of animals urine checking granule |
| CN102215672A (en) * | 2008-11-17 | 2011-10-12 | 雀巢产品技术援助有限公司 | Animal litters |
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| CN103392613B (en) * | 2013-07-24 | 2015-06-17 | 钟春燕 | Cat litter with high water absorption |
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