KR101871629B1 - Ammonia detecting kit and the method of manufacturing same - Google Patents
Ammonia detecting kit and the method of manufacturing same Download PDFInfo
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- KR101871629B1 KR101871629B1 KR1020170030630A KR20170030630A KR101871629B1 KR 101871629 B1 KR101871629 B1 KR 101871629B1 KR 1020170030630 A KR1020170030630 A KR 1020170030630A KR 20170030630 A KR20170030630 A KR 20170030630A KR 101871629 B1 KR101871629 B1 KR 101871629B1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
- G01N33/525—Multi-layer analytical elements
- G01N33/526—Multi-layer analytical elements the element being adapted for a specific analyte
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/84—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving inorganic compounds or pH
Abstract
Description
The present invention relates to a detection kit for detecting ammonia and a detection method.
Conventionally, Berthelot's reaction or Salicylate Method, which causes a specific reaction with ammonia, has been used as a method for detecting ammonia. However, most of these methods occur in the form of liquid phase, and only a water base system such as Deionized Water (D.I. water) can detect only ammonia present in water.
On the other hand, research activities on detecting ammonia in a biological solution such as plasma were insignificant.
Also, conventional methods were not suitable for detecting ammonia in biological solutions, since large amounts of samples were required to detect ammonia in a particular solution. Therefore, it is very important to develop a diagnostic device that can analyze the sample using a small amount of sample obtained from the human body in a shorter time. In particular, there have been few studies on medical and portable kits that can detect ammonia in plasma, and there have been no cases of response in plasma.
One object of the present invention is to detect ammonia in a biological solution such as plasma.
It is another object of the present invention to provide an ammonia detection kit, a kit making method, and an ammonia detection method.
A method of manufacturing an ammonia detection part for one purpose of the present invention includes the steps of adding sodium salicylate and sodium hydroxide to Hydrogel; Drying the hydrogel on a film to prepare a first layer; Adding sodium dichloroisocyanurate and sodium nitrosourexide to a solvent to prepare a mixed solution; And filtering the mixed solution onto paper to prepare the second layer in which the sodium dichloroisocyanurate and sodium nitroprusside are present on the paper.
In one embodiment, the paper may be paper printed with a hydrophobic material, and the film may be a transparent film.
In one embodiment, the solvent may be insoluble for daily use.
For another purpose of the present invention, the ammonia detection unit comprises a first layer comprising sodium salicylate and sodium hydroxide; And a second layer comprising Sodium Dichloroisocyanurate and Sodium Nitroprusside.
In one embodiment, the ammonia detection portion includes hydrogel on the first layer, and the sodium salicylate and sodium hydroxide may be included in the hydrogel.
In one embodiment, the ammonia detection portion may be present on the transparent film including the sodium salicylate and sodium hydroxide, and the hydrogel may be an agarose gel.
In one embodiment, the transparent film may be a PET film (polyethylene terephthalate film).
In one embodiment, the ammonia detection section may comprise paper, the second layer comprises paper, the sodium dichloroisocyanurate and the sodium nitroprusside may be present on the paper, the paper being printed with a hydrophobic material It can be paper.
Another object of the present invention is to provide an ammonia detection kit comprising: a first layer on a transparent film in which a dried hydrogel containing sodium salicylate and sodium hydroxide is present; A second layer comprising dried sodium dicarboxylate and sodium nitroprusside on paper; And a detecting unit including the first layer and the second layer, wherein the detecting unit is formed by overlapping one surface of the second layer on one surface of the first layer where the hydrogel is present.
In one embodiment, the ammonia detection kit may exhibit absorbance or color change when ammonia is detected.
A further object of the present invention is to provide an ammonia detection method comprising: a first layer on a transparent film in the presence of a dried hydrogel comprising sodium salicylate and sodium hydroxide; A second layer comprising dried sodium dicarboxylate and sodium nitroprusside on paper; And a detecting unit including the first layer and the second layer, wherein the detecting unit has one surface of the second layer overlapped on one surface of the first layer where the hydrogel is present, Detection kit; And the ammonia is detected through the color change of the detection unit of the ammonia detection kit.
In one embodiment, the ammonia detection method can confirm the concentration of ammonia through the absorbance or color change of indophenol blue in the detection unit.
In one embodiment, the ammonia detection method can detect the ammonia of the biological solution with the ammonia detection kit.
In one embodiment, the biological solution may be blood plasma.
Through the present invention, ammonia in a biological solution such as plasma can be detected. In particular, unlike the conventional ammonia detection method which requires a large amount of sample, ammonia can be detected with only a small amount of sample. In addition, since the ammonia concentration can be checked by judging the color change, it can be manufactured in a kit form, and can be easily used for medical and portable purposes.
BRIEF DESCRIPTION OF THE DRAWINGS FIG.
Figure 2 is a diagram of forming the second layer of the present invention.
Figure 3 is a diagram of forming the first layer of the present invention.
4 is a view of an ammonia detection kit.
Figure 5 is a diagram for the formation of Indian phenol blue.
6 is a graph showing the results of ammonia detection in the plasma by the ammonia detection method of the present invention.
7 is a view showing the result of detection of ammonia by a conventional method.
8 and 9 are graphs showing the results of a comparative experiment in which ammonia was detected by the detection method of the present invention.
Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. The present invention is capable of various modifications and various forms, and specific embodiments are illustrated in the drawings and described in detail in the text. It is to be understood, however, that the invention is not intended to be limited to the particular forms disclosed, but on the contrary, is intended to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the invention. Like reference numerals are used for like elements in describing each drawing.
The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In the present application, the term "comprises" or "having ", etc. is intended to specify that there is a feature, step, operation, element, part or combination thereof described in the specification, , &Quot; an ", " an ", " an "
Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the contextual meaning of the related art and are to be interpreted as either ideal or overly formal in the sense of the present application Do not.
Ammonia (NH 3 ) is one of the compounds produced by the enterobacteria and the cells of the body in the proteolytic process. When the level of ammonia in the blood increases, liver disease, Reye syndrome, and renal failure Various complications can occur. In addition, if the ammonia in the body is elevated above a certain level, various diseases such as liver cirrhosis of the liver and uremic disorder found in the newborn may occur, resulting in cognitive development problems, seizures, other neurological problems and death. Ammonia should be maintained in the body below 200 ㎛ to prevent diseases in humans. The effect of excessive ammonia concentration on the blood can be summarized as shown in Table 1 below.
(μmol / L)
Examples of underlying causes
(Transient hyperammonemia of the newborn)
Propionic acidaemia
Fatty acid oxidation disorders
(Acquired eg illness, sepsis, liver dysfunction)
It also interferes with the citric acid cycle in the body, causing acute diseases such as difficulty in breathing and coma. Due to the presence of ammonia, glutamate is denatured as glutamine, which causes glutamate deficiency. When glutamate is insufficient, the citric acid circuit is affected by attenuation, making the circuit inoperable. Therefore, it is important to accurately measure the concentration of ammonia in the blood.
Ammonia detection methods include Berthelot's Method or Salicylate Method. Buttero's method is a method proposed by Buttero, using phenol and reacting with ammonia to form indolephenol blue. However, it can only be used in solution and is relatively inaccurate.
There is a salicylate method as a method of stabilizing and improving the method of Butelle. The method of quantifying ammonia by measuring the absorbance of indophenol blue, which is formed by mixing sodium salicylate, sodium hydroxide, sodium dicarboxylate, and sodium nitroprusside, and reacting these compounds with ammonia, It is a high and widely used method. At this time, the final reactant, indophenol blue, absorbs red light of 640 nm and exhibits a blue color. However, the salicylate method was also only used to detect ammonia in solution, and it was impossible to detect ammonia in plasma.
BRIEF DESCRIPTION OF THE DRAWINGS FIG.
Referring to FIG. 1, in order to maximize portability, the present invention provides a dry kit using a salicylate method. Sodium salicylate and sodium hydroxide are added to the hydrogel, which is a hydrophilic polymer, followed by drying, and sodium hypochlorite and sodium nitroprusside are prepared on paper. And if you stack them together, you can detect a drop of plasma ammonia without pretreatment. Therefore, the present invention can be easily used as a plasma ammonia detection sensor which can be used for medical and portable purposes.
Specifically, a method of manufacturing an ammonia detection part for one purpose of the present invention includes the steps of adding sodium salicylate and sodium hydroxide to a hydrogel; Drying the hydrogel on a film to prepare a first layer; Adding sodium dichloroisocyanurate and sodium nitrosourexide to a solvent to prepare a mixed solution; And filtering the mixed solution onto paper to prepare the second layer in which the sodium dichloroisocyanurate and sodium nitroprusside are present on the paper.
In one embodiment, the paper may be paper printed with a hydrophobic material, and the film may be a transparent film.
In one embodiment, the solvent may be insoluble for daily use.
For another purpose of the present invention, the ammonia detection unit comprises a first layer comprising sodium salicylate and sodium hydroxide; And a second layer comprising Sodium Dichloroisocyanurate and Sodium Nitroprusside.
In one embodiment, the ammonia detection unit may include a hydrogel on the first layer, and the sodium salicylate and sodium hydroxide may be included in the hydrogel, and the hydrogel may be an agarose gel.
In one embodiment, the ammonia detection unit may include the sodium hydrogels including sodium salicylate and sodium hydroxide, and the transparent film may be a PET (polyethylene terephthalate) film.
In one embodiment, the ammonia detection portion may comprise paper, the second layer may comprise paper, the sodium dicarboxylate and sodium nitroprusside may be present on the paper, the paper being paper printed with a hydrophobic material .
Figure 2 is a diagram of forming the second layer of the present invention. Referring to FIG. 2, the second layer is prepared by adding sodium dichloroisocyanurate and sodium nitroprusside to toluene. When sodium dichloroisocyanurate and sodium nitrosuronate dissolve in water and then dry, their reactivity drops or disappears. In the present invention, an insoluble solvent, toluene, is used to maintain the reactivity between sodium dichloroisocyanurate and sodium nitroprusside. In toluene, sodium dichloroisocyanurate and nitroprusside sodium are not dissolved but simply released. A toluene solution in which sodium dichloroisocyanurate sodium and nitroprusside sodium are dissolved is filtered with suction paper to form a second layer. The paper uses paper that has been printed with a hydrophobic material to create a hydrophobic zone. The hydrophobic material may be a wax, and in one embodiment, the paper may be paper that has been wax-printed.
Figure 3 is a diagram of forming the first layer of the present invention.
Referring to FIG. 3, the first layer of the present invention is prepared by adding sodium salicylate and sodium hydroxide to the hydrogel. The hydrogel to which sodium salicylate and sodium hydroxide are added is dried over a transparent film to form a first layer. At this time, the hydrogel may use hydrophilic Agarose Gel so that a chemical reaction may occur. And a PET film (polyethylene terephthalate film) can be used as a transparent film.
Another object of the present invention is to provide an ammonia detection kit comprising: a first layer on a transparent film in which a dried hydrogel containing sodium salicylate and sodium hydroxide is present; A second layer comprising dried sodium dicarboxylate and sodium nitroprusside on paper; And a detecting unit including the first layer and the second layer, wherein the detecting unit is formed by overlapping one surface of the second layer on one surface of the first layer where the hydrogel is present.
4 is a view of an ammonia detection kit. Referring to FIG. 4, the ammonia detection kit of the present invention superimposes the first layer and the second layer prepared in a fully dried state. A commercially available adhesive (Commercial Glue) may be used, or a double-sided tape may be used.
In one embodiment, the ammonia detection kit may exhibit absorbance or color change when ammonia is detected.
Figure 5 is a diagram for the formation of Indian phenol blue. Referring to FIG. 5, sodium dicarboxylate sodium thiosulfate serves as a chlorine donor to dissolve chlorine in water. Chlorine forms hypochlorite HOCl in water, which reacts with ammonia in water to form ammonium chloride (Hypochlorite). Ammonium chloride reacts with sodium salicylate to form 5-aminosalicylate. In this case, since the reverse reaction is possible, the surrounding environment is made alkaline to allow 5-amino salicylate to be maintained, and the pH is adjusted to pH 11 by using sodium hydroxide. The other sodium salicylate in the vicinity oxidizes with 5-aminosalicylate and eventually forms indophenol blue. Indian phenol blue absorbs red light (640 nm) and appears blue. This can be achieved through an oxidation catalyst having a cyanide functional group.
A further object of the present invention is to provide an ammonia detection method comprising: a first layer on a transparent film in the presence of a dried hydrogel comprising sodium salicylate and sodium hydroxide; A second layer comprising dried sodium dicarboxylate and sodium nitroprusside on paper; And a detecting unit including the first layer and the second layer, wherein the detecting unit has one surface of the second layer overlapped on one surface of the first layer where the hydrogel is present, Detection kit; And the ammonia is detected through the color change of the detection unit of the ammonia detection kit.
In one embodiment, the ammonia detection method can confirm the concentration of ammonia through the absorption part or color change of indophenol blue in the detection part.
In one embodiment, the ammonia detection method can detect ammonia in a biological solution with the ammonia detection kit.
In one embodiment, the biological solution may be blood plasma.
Hereinafter, embodiments of the present invention will be described in detail. However, the following examples are only a few embodiments of the present invention, and the present invention should not be construed as being limited to the following examples.
[Example]
Materials
PET film (polyethylene terephthalate film, thickness: 0.05 mm): It is a thin and transparent PET film, indicating the formation of indophenol blue.
Agarose LE: A material for making Hydrogel (Mbiotech Company, CAS #: 9012-36-6).
Sodium Salicylate: A material to form Indian phenol blue (Sigma-Aldrich Company, CAS #: 54-21-7).
Sodium Hydroxide, Bead: A material that creates the pH environment of the reaction (Samchun Company, CAS #: 1310-73-2).
Sodium Dichloroisocyanurate: Dissolved in water as a chlorine donor, releasing Cl- (Sigma-Aldrich Company, CAS #: 2893-78-9).
Sodium Nitroprusside: It is dissolved in water to activate the cyanide functional group, which acts as an oxidation catalyst (Sigma-Aldrich Company, CAS #: 13755-38-9).
Paper preparation
Wax printing was performed on paper (filter paper) using a ColorCube 8570 model printer from Xerox Corporation (Color cube 8570 model Printer). The wax-free inner diameter was 2 mm. The inner diameter was 2 mm, 3 mm, 4 mm, and 5 mm, respectively. Wax-printed
Made Hydrogel
0.5 w / v% Concentration (w / v.%) 250 mg Agarose LE was added to 50 ml deionized water (D.I.water) to make an agarose hydrogel. The deionized water to which Agarose LE was added was heated in a microwave oven (microwave oven) for 2 minutes to confirm that it was completely dissolved, stirred with a magnetic stirrer, and slowly cooled at room temperature. While the agarose hydrogel was cooled, 2.523 mg and 482.770 mg of sodium salicylate and sodium hydroxide were respectively prepared. When the agarose hydrogel slowly cooled to room temperature at 40 占 폚, sodium salicylate and sodium hydroxide which had been prepared were slowly added thereto and stirred until completely dissolved. When all of the added chemicals were dissolved, the mixture was quantitated to 20 탆 and placed on a transparent PET film to be completely dried.
Preparation of Sodium Dichloroisocyanurate and Nitroprulose Sodium
In the present invention, an insoluble solvent, toluene, is used to maintain the reactivity between sodium dichloroisocyanurate and sodium nitroprusside. In toluene, the chemicals do not dissolve and are simply released. Sodium dicarboxylate and sodium nitroprusside were weighed at 304 mg and 16 mg, respectively, and finely ground in a mortar. The chemical compound was added to the toluene at a concentration of 5 mg / mL.
Chemical transfer with toluene to paper printed with hydrophobic material
It is necessary to transfer sodium sodium isocyanurate and sodium nitrosuronate to the center of the wax-printed paper using a toluene solution in which sodium dichloroisocyanurate and sodium nitrosulphate are dissolved. For this purpose, wax-printed paper was prepared in a suction state. The toluene is sent down through the suction and can be dried quickly due to the air flow. Toluene chemicals were filtered through wax-printed paper through this filtering process and transferred to paper. The amount of chemicals transferred to the paper was 0.75 mg. Since the sodium isocyanurate chloride and sodium nitroprusside were released at a concentration of 5 mg / mL, the amount of toluene was filtered using 150 占 퐇.
Plasma Ammonia detection through two completely dried layers
The chemical composition of the final two layers is as follows.
1 layer: Agarose LE 50 占 퐂, sodium salicylate 504.6 占 퐂, sodium hydroxide 96.55 占 퐂
2 layer: sodium dicarboxylate dihydrate: 712.5 占 퐂, nitroprusside sodium: 37.5 占 퐂
One side of the two layers was stacked and fixed and made into one kit. One drop (40 μl) of the sample to be detected was dropped on the inner diameter portion of the wax-free inner surface of the paper in the center of the paper in the fully dried state, and a reaction time of 10 minutes was provided.
6 is a graph showing the results of ammonia detection in the plasma by the ammonia detection method of the present invention. Referring to FIG. 6, it was observed that the indent phenol blue was formed through the PET film in the lower part of the kit, and the color changed to blue.
[Experimental Example]
A comparative experiment was conducted on the ammonia detection according to the present invention.
Comparative experiment with conventional method
First, the conventional method is a method of detecting ammonia by adding sodium salicylate, sodium hydroxide, dicarboxylic acid and nitroprulose to toluene, which is used in the salicylate method. For comparison with the present invention, The mixed solution of toluene formed by the method was tested on paper by filtering.
7 is a view showing the result of detection of ammonia by a conventional method. Referring to FIG. 7, in the experiment using the deionized water-based sample, it was confirmed that the color changed to blue and ammonia was detected, but no change was observed in the experiment using 100% human plasma without pretreatment .
Parameters Affecting the Formation of Indian Phenol Blue 1
8 is a graph showing the results of a comparative experiment in which ammonia was detected by the detection method of the present invention. Referring to FIG. 8, it was confirmed that the formation of indophenol blue was influenced by the amount of sodium salicylate as a result of an experiment in which only the amount of sodium salicylate differed from the other variables. Sodium salicylate is the main chemical that forms indophenol blue. When the amount of sodium salicylate is insufficient, the formation of indolene phenol blue as a final product is reduced, and the ability to detect ammonia is lowered. Specifically, the amounts of sodium hydroxide, sodium dichloroisocyanurate and sodium nitrosuccinate were fixed to 96.554, 712.5, and 37.5, respectively. The amount of salicylic acid was increased from left to 252.3508 ㎍, 336.4676 ㎍ to the rightmost one to 420.5846 ㎍. Test samples were tested using 40 ㎕ of normal plasma. After a reaction time of 10 minutes, it was observed that Indian phenol blue was formed more as the amount of sodium salicylate increased, indicating that the formation of Indian phenol blue was directly proportional to the amount of sodium salicylate.
Parameters Affecting the Formation of
9 is a graph showing the results of a comparative experiment in which ammonia was detected by the detection method of the present invention. Referring to FIG. 9, it was confirmed that the amount of sodium dichloroisocyanurate and the amount of nitroprulose dispersed in toluene in addition to the sodium salicylate was varied, and as a result, it was confirmed that there was an optimized amount. Specifically, the amounts of sodium salicylate and sodium hydroxide were fixed to 420.5846 ㎍ and 96.554 ㎍, respectively, and the amount of sodium dichloroisocyanurate and sodium nitrosulphate was increased to confirm the formation of indene phenol blue. From the left side, the amounts of sodium dichloroisocyanurate and nitroprusside sodium were increased to 0.475 mg and 0.025 mg, 0.7125 mg and 0.0375 mg, 0.95 mg and 0.05 mg, 1.1875 mg and 0.0625 mg, respectively. The optimum amount of sodium dichloroisocyanurate and nitroprusside sodium was found to be 0.7125 mg and 0.0375 mg, respectively.
It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the present invention as defined by the following claims. It can be understood that it is possible.
Claims (17)
Drying the hydrogel on a film to prepare a first layer;
Adding sodium dichloroisocyanurate and sodium nitrosourexide to a solvent to prepare a mixed solution; And
Filtering said mixed solution onto paper to prepare said second layer wherein said sodium dichloroisocyanurate and sodium nitroprusside are present on said paper;
A method for producing ammonia detection part.
The paper is a paper printed with a hydrophobic material,
A method for producing ammonia detection part.
Wherein the film is a transparent film,
A method for producing ammonia detection part.
Wherein the solvent is an insoluble solvent,
A method for producing ammonia detection part.
A second layer comprising Sodium Dichloroisocyanurate and Sodium Nitroprusside, and a second layer comprising Sodium Dichloroisocyanurate and Sodium Nitroprusside.
Ammonia detection.
Wherein the first layer comprises a hydrogel, and the sodium salicylate and sodium hydroxide are contained in the hydrogel.
Ammonia detection.
The hydrogel comprising sodium salicylate and sodium hydroxide is present on the transparent film,
Ammonia detection.
The hydrogel may be an agarose gel,
Ammonia detection.
The transparent film may be a PET film (polyethylene terephthalate film)
Ammonia detection.
Characterized in that said second layer comprises paper, said sodium dichloroisocyanurate and sodium nitroprusside being present on said paper.
Ammonia detection.
Wherein the paper is a paper printed with a hydrophobic substance,
Ammonia detection.
A second layer comprising dried sodium dicarboxylate and sodium nitroprusside on paper; And
And a detection unit including the first layer and the second layer,
Wherein the detecting unit is formed by overlapping one surface of the second layer on one side of the first layer where the hydrogel is present,
Ammonia detection kit.
When ammonia is detected, absorbance or color change appears,
Ammonia detection kit.
A second layer comprising dried sodium dicarboxylate and sodium nitroprusside on paper; And
And a detection unit including the first layer and the second layer,
Wherein the detecting unit is formed by overlapping one surface of the second layer on one surface of the first layer where the hydrogel is present.
Wherein ammonia is detected through the detection unit of the ammonia detection kit,
Ammonia detection method.
The concentration of ammonia is determined by the absorbance or color change of indophenol blue in the detection unit.
Ammonia detection method.
The ammonia detection kit detects ammonia of a biological solution,
Ammonia detection method.
Wherein the biological solution is a plasma plasma,
Ammonia detection method.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| KR101925544B1 (en) * | 2018-07-02 | 2018-12-05 | 주식회사 코비 | Ammonia measuring system having auto-backwashing pretreatment apparatus and constant-temperature detector |
| CN111175237A (en) * | 2020-01-09 | 2020-05-19 | 中国神华煤制油化工有限公司 | Sodium salicylate spectrophotometric method for measuring ammonia nitrogen in water |
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| KR20170014073A (en) * | 2015-07-28 | 2017-02-08 | 성균관대학교산학협력단 | Film for detecting harmful material with enhanced detection sensitivity and the method of preparing the same |
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| KR20130009006A (en) * | 2011-07-14 | 2013-01-23 | 서울시립대학교 산학협력단 | Nitrogen ammonia concentration measuring agent and the kit using thereof |
| KR20170014073A (en) * | 2015-07-28 | 2017-02-08 | 성균관대학교산학협력단 | Film for detecting harmful material with enhanced detection sensitivity and the method of preparing the same |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101925544B1 (en) * | 2018-07-02 | 2018-12-05 | 주식회사 코비 | Ammonia measuring system having auto-backwashing pretreatment apparatus and constant-temperature detector |
| CN111175237A (en) * | 2020-01-09 | 2020-05-19 | 中国神华煤制油化工有限公司 | Sodium salicylate spectrophotometric method for measuring ammonia nitrogen in water |
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