CN114616467B - 筛选原代人类细胞中的同源t细胞和表位反应性的高通量方法 - Google Patents

筛选原代人类细胞中的同源t细胞和表位反应性的高通量方法

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Publication number
CN114616467B
CN114616467B CN202080070295.3A CN202080070295A CN114616467B CN 114616467 B CN114616467 B CN 114616467B CN 202080070295 A CN202080070295 A CN 202080070295A CN 114616467 B CN114616467 B CN 114616467B
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antigen
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CN114616467A (zh
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迪林·拉奎尔
哈尼·克安库
普尔普·史蒂芬
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Regeneron Pharmaceuticals Inc
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    • A61K40/00—Cellular immunotherapy
    • A61K40/10—Cellular immunotherapy characterised by the cell type used
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CN202080070295.3A 2019-10-03 2020-10-02 筛选原代人类细胞中的同源t细胞和表位反应性的高通量方法 Active CN114616467B (zh)

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US201962910379P 2019-10-03 2019-10-03
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PCT/US2020/054125 WO2021067851A1 (en) 2019-10-03 2020-10-02 High-throughput method to screen cognate t cell and epitope reactivities in primary human cells

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Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20240167082A1 (en) * 2021-03-19 2024-05-23 Paris Sciences Et Lettres Methods for the selective analysis of cells or organelles
EP4337955A4 (en) * 2021-05-13 2025-07-23 La Jolla Inst For Immunology CORONAVIRUS T-CELL EPITOPES, MEGAPOOLS, AND RELATED USES
CN113980899A (zh) * 2021-11-29 2022-01-28 杭州艾沐蒽生物科技有限公司 一种高通量筛选抗原特异性tcr的方法
CN117286099A (zh) * 2022-06-23 2023-12-26 富禾生医股份有限公司 用于诱导抗原特异性t细胞的方法及其在癌症或病毒感染治疗中的用途
WO2024092740A1 (zh) * 2022-11-04 2024-05-10 深圳华大生命科学研究院 检测细胞间相互作用的方法及其应用
GB202302709D0 (en) * 2023-02-24 2023-04-12 Genome Res Ltd Method of identifying t cell recpetors of interest
KR20260026101A (ko) * 2023-05-23 2026-02-25 주노 쎄러퓨티크스 인코퍼레이티드 T 세포의 활성화 마커 및 t 세포 활성화를 평가하는 방법
EP4720109A1 (en) 2023-05-25 2026-04-08 Regeneron Pharmaceuticals, Inc. T cell receptors that bind presented hpv16-, mart1-, cmv-, ebv-, or influenza- peptides
WO2025064761A1 (en) 2023-09-22 2025-03-27 Regeneron Pharmaceuticals, Inc. Kras10-18 g12d off-target peptides and uses thereof
WO2025064738A1 (en) 2023-09-22 2025-03-27 Regeneron Pharmaceuticals, Inc. Dntt 250-258 off-target peptides and uses thereof
CN117126949B (zh) * 2023-10-26 2024-05-03 北京大学第三医院（北京大学第三临床医学院） 一种鉴定羊水样本中原代细胞的细胞类型和溯源的方法
WO2026006724A1 (en) 2024-06-28 2026-01-02 Regeneron Pharmaceuticals, Inc. Prame off-target peptides and uses thereof
WO2026006734A1 (en) 2024-06-28 2026-01-02 Regeneron Pharmaceuticals, Inc. Off-target peptide-mhc complex conformation modeling systems and methods for antigen-recognition molecule development

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105358984A (zh) * 2013-03-15 2016-02-24 普罗格诺西斯生物科学公司 用于检测肽/mhc/tcr结合的方法
CN110198954A (zh) * 2017-01-13 2019-09-03 艾吉纳斯公司 与ny-eso-1结合的t细胞受体和其使用方法

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5071A (en) 1847-04-17 George page
US166A (en) 1837-04-17 Standing press
US419A (en) 1837-10-06 Machine fob boring and mortising wheel-hubs and other articles
US743A (en) 1838-05-17 Improvement in plows
US5932A (en) 1848-11-21 brown
US5789A (en) 1848-09-19 Improvement in fountain-pen holders and nibs
US5702931A (en) 1991-07-01 1997-12-30 Berlex Laboratories, Inc. Mutagenesis methods and compositions
JP2000512142A (ja) 1996-06-07 2000-09-19 マサチューセッツ インスティチュート オブ テクノロジー プログラムされた連続的変異誘発
US5780270A (en) 1996-07-17 1998-07-14 Promega Corporation Site-specific mutagenesis and mutant selection utilizing antibiotic-resistant markers encoding gene products having altered substrate specificity
AU2016206280B2 (en) * 2001-12-07 2018-05-17 Asterias Biotherapeutics, Inc. Hematopoietic cells from human embryonic stem cells
CA2501870C (en) 2002-10-09 2013-07-02 Avidex Limited Single chain recombinant t cell receptors
ES2971647T3 (es) 2005-04-15 2024-06-06 Macrogenics Inc Diacuerpos covalentes y usos de los mismos
US9963510B2 (en) 2005-04-15 2018-05-08 Macrogenics, Inc. Covalent diabodies and uses thereof
EP2486049A1 (en) 2009-10-06 2012-08-15 The Board Of Trustees Of The UniversityOf Illinois Human single-chain t cell receptors
US11203783B2 (en) * 2013-11-21 2021-12-21 Repertoire Genesis Incorporation T cell receptor and B cell receptor repertoire analysis system, and use of same in treatment and diagnosis
SG10202005892SA (en) * 2014-06-06 2020-07-29 Herlev Hospital Determining antigen recognition through barcoding of mhc multimers
EP3234130B1 (en) * 2014-12-19 2020-11-25 The Broad Institute, Inc. Methods for profiling the t-cell- receptor repertoire
EP4248744A3 (en) * 2015-04-06 2023-12-27 Regeneron Pharmaceuticals, Inc. Humanized t cell mediated immune responses in non-human animals
MX2018003532A (es) * 2015-09-24 2019-04-25 Abvitro Llc Conjugados de afinidad-oligonucleotido y usos de los mismos.
CN117887804A (zh) 2017-02-02 2024-04-16 纽约基因组研究中心公司 用于识别或量化在生物样品中的靶标的方法和组合物
WO2019113506A1 (en) * 2017-12-07 2019-06-13 The Broad Institute, Inc. Methods and compositions for multiplexing single cell and single nuclei sequencing

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105358984A (zh) * 2013-03-15 2016-02-24 普罗格诺西斯生物科学公司 用于检测肽/mhc/tcr结合的方法
CN110198954A (zh) * 2017-01-13 2019-09-03 艾吉纳斯公司 与ny-eso-1结合的t细胞受体和其使用方法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Cell Hashing with barcoded antibodies enables multiplexing and doublet detection for single cell genomics;Marlon Stoeckius等;《GenomeBiology》;20181231;第19卷(第224期);1-12 *
Isolation of T-Cell Receptors Specifically Reactive with Mutated Tumor-Associated Antigens from Tumor-Infiltrating Lymphocytes Based on CD137 Expression;Maria Parkhurst等;《Clinical Cancer Research》;20170515;第23卷(第10期);2491-2505 *
Overview of methodologies for T-cell receptor repertoire analysis;Elisa Rosati等;《BMC Biotechnology》;20171231;第17卷(第61期);1-16 *

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WO2021067851A1 (en) 2021-04-08
AU2020358975A1 (en) 2022-04-21
US20210102942A1 (en) 2021-04-08
KR20220078611A (ko) 2022-06-10
JP2025105774A (ja) 2025-07-10
JP7711049B2 (ja) 2025-07-22
CA3153119A1 (en) 2021-04-08
CN114616467A (zh) 2022-06-10
JP2022552151A (ja) 2022-12-15
EP4041868A1 (en) 2022-08-17
IL291859A (en) 2022-06-01

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