CN114557360B - Ternary compound bactericide for preventing and treating hybrid rice seed production field rice grain smut and application thereof - Google Patents
Ternary compound bactericide for preventing and treating hybrid rice seed production field rice grain smut and application thereof Download PDFInfo
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- 241000209094 Oryza Species 0.000 title claims abstract description 85
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- 235000009566 rice Nutrition 0.000 title claims abstract description 85
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 53
- 239000003899 bactericide agent Substances 0.000 title claims abstract description 53
- 235000013339 cereals Nutrition 0.000 title claims abstract description 40
- 150000001875 compounds Chemical class 0.000 title claims abstract description 32
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 23
- 239000005802 Mancozeb Substances 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- STMIIPIFODONDC-UHFFFAOYSA-N 2-(2,4-dichlorophenyl)-1-(1H-1,2,4-triazol-1-yl)hexan-2-ol Chemical compound C=1C=C(Cl)C=C(Cl)C=1C(O)(CCCC)CN1C=NC=N1 STMIIPIFODONDC-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000005730 Azoxystrobin Substances 0.000 claims abstract description 16
- WFDXOXNFNRHQEC-GHRIWEEISA-N azoxystrobin Chemical compound CO\C=C(\C(=O)OC)C1=CC=CC=C1OC1=CC(OC=2C(=CC=CC=2)C#N)=NC=N1 WFDXOXNFNRHQEC-GHRIWEEISA-N 0.000 claims abstract description 16
- 239000002904 solvent Substances 0.000 claims abstract description 3
- 239000000417 fungicide Substances 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 13
- 241000196324 Embryophyta Species 0.000 claims description 11
- 230000000855 fungicidal effect Effects 0.000 claims description 10
- 238000005507 spraying Methods 0.000 claims description 10
- 230000035784 germination Effects 0.000 abstract description 28
- 238000003306 harvesting Methods 0.000 abstract description 3
- 238000009331 sowing Methods 0.000 abstract description 2
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- 239000003814 drug Substances 0.000 description 10
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- 239000004495 emulsifiable concentrate Substances 0.000 description 4
- 238000010899 nucleation Methods 0.000 description 4
- 239000005760 Difenoconazole Substances 0.000 description 3
- 239000005822 Propiconazole Substances 0.000 description 3
- BQYJATMQXGBDHF-UHFFFAOYSA-N difenoconazole Chemical compound O1C(C)COC1(C=1C(=CC(OC=2C=CC(Cl)=CC=2)=CC=1)Cl)CN1N=CN=C1 BQYJATMQXGBDHF-UHFFFAOYSA-N 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000000575 pesticide Substances 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- STJLVHWMYQXCPB-UHFFFAOYSA-N propiconazole Chemical compound O1C(CCC)COC1(C=1C(=CC(Cl)=CC=1)Cl)CN1N=CN=C1 STJLVHWMYQXCPB-UHFFFAOYSA-N 0.000 description 3
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- 230000009286 beneficial effect Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 230000007226 seed germination Effects 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- WURBVZBTWMNKQT-UHFFFAOYSA-N 1-(4-chlorophenoxy)-3,3-dimethyl-1-(1,2,4-triazol-1-yl)butan-2-one Chemical compound C1=NC=NN1C(C(=O)C(C)(C)C)OC1=CC=C(Cl)C=C1 WURBVZBTWMNKQT-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000221198 Basidiomycota Species 0.000 description 1
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 229930191978 Gibberellin Natural products 0.000 description 1
- 241000011383 Tilletia horrida Species 0.000 description 1
- 241000221566 Ustilago Species 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 239000006013 carbendazim Substances 0.000 description 1
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
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- 230000002349 favourable effect Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 1
- 239000003448 gibberellin Substances 0.000 description 1
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- 230000001225 therapeutic effect Effects 0.000 description 1
- YFNCATAIYKQPOO-UHFFFAOYSA-N thiophanate Chemical compound CCOC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OCC YFNCATAIYKQPOO-UHFFFAOYSA-N 0.000 description 1
- 238000012876 topography Methods 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/12—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
- A01N47/14—Di-thio analogues thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A90/00—Technologies having an indirect contribution to adaptation to climate change
- Y02A90/40—Monitoring or fighting invasive species
Abstract
The invention discloses a ternary compound bactericide for preventing and treating rice grain smut in hybrid rice seed production fields and application thereof, wherein the ternary compound bactericide uses water as a solvent and contains 200-2000 mg.L of mancozeb ‑1 50-1000 mg.L hexaconazole ‑1 Azoxystrobin 100-2000 mg.L ‑1 . The ternary compound bactericide for preventing and treating the rice grain smut in the hybrid rice seed production field provided by the invention has no influence on related indexes such as thousand seed weight, seed setting rate, germination rate and the like while effectively preventing and treating the rice grain smut, and simultaneously ensures seed yield and sowing quality after harvesting.
Description
Technical Field
The invention relates to the technical field of sterilization compositions, in particular to a low-toxicity, high-efficiency and environment-friendly ternary compound bactericide, and particularly relates to an application for preventing and controlling rice grain smut in hybrid rice seed production fields.
Background
Rice kernel smut (Rice kernel smut) is a fungal Rice disease caused by the soil-borne fungus basidiomycete T illetia horrida, and is most serious in southeast Asia worldwide, and occurs in Rice producing areas in both south and north China, so that the occurrence of late Rice is more. Since the 70 th century of 20 th, hybrid rice has been continuously popularized and promoted in China, and the damage to hybrid rice fields is serious due to the high stigma exposure rate, long Zhang Ying time and large Zhang Ying angle of sterile line varieties used in hybrid rice seed production, which is favorable for invasion of the bacteria, one of important diseases (Ding Shifeng, chen Hua, zhu Xiaobo, wu Haixia, che Jin English, kangya and Cui Guhua. Control effect test of several common bactericides on hybrid seed production rice grain smut [ J ]. Anhui agronomic report, 2020,26 (24): 116-117.). Rice grain smut infects in the flowering phase, affects the yield and quality of hybrid rice seeds by producing large amounts of black powdery winter spores, and annual prevalence rates are as high as 40% to 60%, which can result in 5% to 20% loss of yield in rice seed fields (Na Wang et al draft Genome Sequence of the Rice Kernel S mut Tilletia horrida Strain QB-1[ j ]. Genome Announcements,2015,3 (3): e00621-15-e 00621-15.). The disease is a climatic disease, which occurs in the stage of raising flowers to maturing of rice, and in this time, the summer and autumn systems often meet typhoon weather or rainy weather after autumn, and the disease is suitable for high temperature and high humidity, and is very beneficial to the occurrence of rice grain smut disease (Zhang Banghua. The occurrence and prevention of the rice grain smut disease in hybrid rice seed fields [ J ]. Phytoscope 2018,31 (07): 56-57.).
In the past, chemical control has been mainly used to control rice grain smut, and more bactericides such as carbendazim, thiophanate and triadimefon have been used in the last 80 th century (Hu Miao, xue Taipu. Research on occurrence rules and control of rice grain smut [ J. ]. Protect of plants, 1987 (02): 87-92.). However, the long-term use of single chemical pesticides is very easy to cause pathogenic bacteria to generate drug resistance, and compared with the single chemical pesticides, the compound bactericide has obvious synergism on the prevention and control of plant diseases (Bi Qiuyan. The compound synergism mechanism of the binary bactericide is first discovered [ D ]. Hebei university of agriculture, 2010.). The method comprises the steps of using more compound medicaments such as "Ke Heijing" and "Miao Hei No. 1" in the 90 th century (Miao Jianwei. Experiments for controlling the rice grain smut disease of hybrid rice seed production fields by different medicaments are summarized [ J ]. Hybrid rice, 1996 (S1): 12-13.), and using more compound medicaments such as "love seedlings" and "Fu Liku" in the beginning of the 21 st century (Wang Jianlong, xu Shuyun, xia Zongxin, yan Bo. Research on controlling the rice grain smut disease of hybrid rice seed production medicaments [ J ]. Hunan agricultural science, 2005 (03): 46-49.).
At present, chemical agents for preventing and treating rice grain smut are mainly seedlings (30% difenoconazole propiconazole EC) which have certain preventing and treating effects on rice sheath blight and rice grain smut, but have a history of use for many years and lack new rice grain smut preventing and treating agents. In addition, research on prevention and treatment of the gramineous Ustilago oryzae by using ternary compound medicaments is not reported yet.
Disclosure of Invention
The invention provides a ternary compound bactericide for preventing and treating rice grain smut in hybrid rice seed production fields and application thereof, which can effectively reduce morbidity and improve prevention and treatment effects, can avoid the drop of thousand-grain weight and germination rate of seeds, and is simple in application method.
The specific technical scheme is as follows:
the invention provides a ternary compound bactericide for preventing and controlling rice grain smut in hybrid rice seed production fields, which takes water as solvent and contains 200-2000 mg.L of mancozeb -1 50-1000 mg.L hexaconazole -1 Azoxystrobin 100-2000 mg.L -1 。
Further, the ternary compound bactericide contains 740-750mg.L mancozeb -1 190-210 mg.L hexaconazole -1 240 to 250 mg.L of azoxystrobin -1 。
Furthermore, the ternary compound bactericide contains mancozeb 747mg.L -1 200 mg.L of hexaconazole -1 250 mg.L of azoxystrobin -1 。
The invention also provides application of the ternary compound bactericide in preventing and controlling rice grain smut in hybrid rice seed production fields.
The invention also provides a preparation method of the ternary compound bactericide, which comprises the following steps: the mancozeb, the hexaconazole and the azoxystrobin are directly dissolved in water, and after being uniformly stirred, the ternary compound bactericide for preventing and controlling the rice grain smut of hybrid rice seed fields is obtained.
The invention also provides a method for preventing and controlling the black powder disease of the rice grains in the hybrid rice seed production field, which comprises the following steps: the ternary compound bactericide is adopted to perform twice pesticide application on plants; first application: uniformly spraying on the ear layers of the parent plants 2d before heading the female parent; second application: uniformly spraying on the scion layer of the female parent plant in the full-period of female parent scion.
Further, the interval between two application is 5-7 d; the heading full period is a period in which the heading rate reaches 45% -55%.
Further, the spraying amount of the ternary compound bactericide is 30-40L (mu. Times) -1 。
Compared with the prior art, the invention has the following beneficial effects:
(1) The ternary compound bactericide for preventing and treating the rice grain smut in the hybrid rice seed production field contains protective bactericide mancozeb, protective and therapeutic bactericide hexaconazole and novel efficient bactericide azoxystrobin, can effectively prevent the rice grain smut and simultaneously effectively avoid the drop of thousand seed weight and germination rate of seeds, and ensures the quality of the harvested seeds.
(2) The ternary compound bactericide for preventing and treating the rice grain smut in the hybrid rice seed production field provided by the invention takes water as a matrix, does not contain any organic solvent, is environment-friendly, has a simple production process, is low in medicine consumption and low in cost, and is suitable for popularization.
Detailed Description
The invention will be further described with reference to the following examples, which are given by way of illustration only, but the scope of the invention is not limited thereto.
In the following examples, the hybrid rice seed production combination ' Zhejie 8 ' female parent is Zhejie A, the male parent is T-8, and the hybrid rice belongs to indica type three-line hybrid rice, and is combined and bred by China institute of rice, zhejiang Don ' T forget agriculture Co., ltd; 'Huazheu 261' female parent is 'Huazhe 2A', male parent is '16T', and belongs to indica type three-line hybrid rice, and the hybrid rice is combined and bred by Zhejiang Don forget agriculture Co., ltd; both hybrid combination parental materials were provided by Zhejiang Don forget agriculture, inc.
80% mancozeb WP was purchased from Dow Yinong agricultural science and technology (China); 250g·L -1 Azoxystrobin SC was purchased from n-danan crop protection limited; 30% hexaconazole SC was purchased from Shanghai Biopharmaceutical Co. The control agent loving seeding (30% difenoconazole propiconazole) emulsifiable concentrate is purchased from Xianzhengda (China) investment Limited company.
Example 1
1. Test material: hybrid rice seed production combination Zheyou 8: the female parent is 'Zhongzhe A', and the male parent is 'T-8'.
2. Test agent: 80% mancozeb WP, 250 g.L -1 Azoxystrobin SC, 30% hexaconazole SC and lovely seeding (30% difenoconazole propiconazole) emulsifiable concentrate.
3. Test method
Experiments were carried out in 2020 and 2021 on hybrid rice seed fields of Seischio division, a company of agricultural industry, inc. of Zhejiang Don't forget, respectively, and stage sowing was carried out according to the growth period characteristics of the parents. Parent-parent row ratio 2:8 at transplanting, parent-parent interval 25cm; the male parent is double-row, the row spacing is 24cm, the plant spacing is 24cm, and the proportion of the male parent in the first period and the male parent in the second period in each row is 1:1, 2 of the male parent is inserted; the female parent is planted closely, the planting density is 15cm (plant spacing) ×22.5cm (row spacing), and 5 grains of rice seedling are planted in each root. Gibberellin is sprayed in batches after heading to ensure that the parent flowers meet and the outcrossing fruiting rate is improved.
Preparation and treatment of the medicament:
in 2020:
weighing 14g of 80% mancozeb WP and 10g of 30% hexaconazole SC, and dissolving in 15L of water to obtain (1-1) which can be directly used; weighing 14g of 80% mancozeb WP, and weighing 250 g.L by using a measuring cylinder -1 30ml of azoxystrobin SC is dissolved in 15L of water to obtain (1-2) which can be directly used; measuring 250 g.L by using a measuring cylinder -1 45ml of azoxystrobin SC is dissolved in 15L of water to obtain azoxystrobin single agent (2-1) which can be directly used; weighing 15g of 30% hexaconazole SC, and dissolving in 15L of water to obtain single hexaconazole (2-2) which can be directly used; weighing 18.75g of 80% mancozeb WP, and dissolving in 15L of water to obtain a mancozeb single agent (2-3) which can be directly used; control one: weighing 5ml of the loving seeding emulsifiable concentrate, and dissolving in 15L of water (CK-1); control two: clear water (CK-2). The treatment and the control are sprayed according to the dosage of 30L per mu. Specific field test agentThe spray concentrations are shown in Table 1.
2021:
weighing 80% mancozeb WP 14g,30% hexaconazole SC 10g, and measuring 250 g.L by using a cylinder -1 15ml of azoxystrobin SC is dissolved in 15L of water to obtain a ternary compound bactericide (containing mancozeb 747 mg.L) which can be directly used -1 200 mg.L of hexaconazole -1 250 mg.L of azoxystrobin -1 ) The method comprises the steps of carrying out a first treatment on the surface of the Weighing 14g of 80% mancozeb WP and 10g of 30% hexaconazole SC, dissolving in 15L of water, and combining as a binary medicament (1-1); weighing 14g of 80% mancozeb WP, and weighing 250 g.L by using a measuring cylinder -1 30ml of azoxystrobin SC is dissolved in 15L of water and used as a binary medicament combination II (1-2); control one: weighing 5ml of the loving seeding emulsifiable concentrate, and dissolving in 15L of water (CK-1); control two: clear water (CK-2). The treatment and the control are sprayed according to the dosage of 30L per mu. The specific spray concentrations of the field test agents are shown in Table 2.
Table 1 field treatment number and chemical treatment concentration in 2020
Table 2 2021 field treatment number and treatment concentration
Each treatment in 2020-2021 is repeated for 3 times, the field fertility level and the topography are combined, a random block design is adopted, the length of a single cell in the north-south direction is about 7m, the width of the single cell in the east-west direction is about 2.3m, and the area of the single cell is 16.1m 2 。
Spraying time and method: spraying a parent ear layer 2d before heading of the female parent for the first time; the second application is carried out by spraying the female parent ear layer in the female parent ear full period (about 50% of ear) and the interval between the two applications is 7d.
The sampling method comprises the following steps: adopting a five-point sampling method, taking 25 bags of rice ears in each district, packaging by using a net bag, hanging in a net room, ventilating and airing, threshing, calculating the seed setting rate, disease grain rate and prevention effect, and measuring the indexes such as thousand grain weight, germination vigor and germination rate.
The measurement of the index such as thousand seed weight, germination potential and germination percentage is carried out by reference to the regulation of the crop seed test procedure (GB/T3543, 1995). The germination test was performed using 100 pieces of paper for germination, and repeated three times per box. The germination condition is that the germination is carried out at 20 ℃ in dark for 16 h/at 30 ℃ and the germination is carried out by illumination for 8h. The number of germinated seeds was recorded from the next day of seed setting, the germination vigor was calculated at 4d, and the germination rate was calculated at 7d.
Statistical analysis: statistical analysis of the data using SPSS 22.0 software, differential significance analysis (P < 0.05) was performed on the test data using Duncan's new complex polar error method.
4. Experimental results
In the experiment of 2020 in this example, since the disease in the field plot is light, the disease particle rate of each treatment is not high as a whole, but after treatment of Zheyou 8 in 3 single-dosage bactericides and 2 binary compound bactericides, the disease particle rate is obviously reduced compared with CK-2; wherein the disease granule rate of 2-1 treatment is the lowest and is 3.45%; the disease grain rate and the relative CK-2 prevention effect of each treatment are not obviously different from those of the loved seedling (CK-1). Thus, in the experiment of this example 2020, there was no significant synergy after binary compounding of the 3 single-dose fungicides (table 3). In addition, after treatment of Zheyou No. 8 in 3 single-dosage bactericides and 2 binary compound bactericides, the thousand-grain weight and the setting rate of the Zheyou No. 8 are not obviously influenced, and the treatment is not obviously different from the treatment of 2 controls.
The harvested seeds were dried and subjected to germination tests, which showed no significant differences in germination vigor, germination rate, seedling height and root length compared to the two controls. Illustrating that the Zhejiang you No. 8F after the treatment of the bactericides 1 The vigor and vigor of the seed generation were not adversely affected (Table 4).
Table 3 effect of the treatment with the pharmaceutical preparation on controlling the smut of Zhejiang you No. 8 rice grain
Note that the different lowercase letters after the data represent the significance of the differences between the same column of data (P < 0.05). The following is the same.
TABLE 4 influence of the treatment with the agent on the germination index of seeds of Zhejie No. 8
In the test of 2021 in this example, after the ternary complex fungicide is treated, the grain rate of the rice grain black powder disease is obviously lower than that of 2 binary complex fungicides and 2 controls, the relative CK-2 control effect and the relative CK-1 control effect respectively reach 68.37% and 51.38%, and are obviously better than that of 2 binary complex fungicides of hexaconazole-mancozeb (1-1) and azoxystrobin-mancozeb (1-2), which shows that the ternary complex fungicide has obvious synergistic effect compared with the binary complex fungicide and is also obviously better than that of the current field common agent loving seedling (CK-1). The test results simultaneously show that the ternary complex fungicide of the invention has no obvious influence on thousand seed weight and seed setting rate of hybrid rice seeds (Table 5).
The germination test is carried out after the seeds are dried, and the results show that the germination vigor, germination rate, seedling root length and seedling height of the seeds after the ternary complex bactericide treatment have no obvious difference compared with those of the water treatment (CK-2), which indicates that the ternary complex bactericide has no obvious influence on the viability and vitality of the seeds (Table 6).
Table 5 influence of ternary combination bactericide on thousand seed weight, setting rate, disease rate and control efficiency of Zheyou No. 8' in hybrid rice
Table 6 Effect of ternary combination Bactericide on seed germination index after harvesting Zheyou No. 8 in hybrid Rice
Example 2
In the embodiment 1, it is confirmed that the ternary compound bactericide can obviously reduce the disease particle rate of the rice grain smut in the hybrid rice seed production field, the control effect is obviously better than that of the loving seedling (CK-1) and the viability and vitality of seeds are not influenced, and the application effect and the influence on the quality of the seeds of the ternary compound bactericide are further verified in the embodiment.
1. Test material: hybrid rice seed production combination 'Huazheuyou 261': the female parent is "Huazhe 2A", and the male parent is "16T".
2. Test agent: as in example 1.
3. Test method
Experiments were carried out in the hybrid rice seed fields of Sechang, inc. of Zhejiang Don forget agriculture, inc. in 2020 and 2021, respectively, and the parental planting method, the experimental design, the cell area, the preparation and treatment of the pharmaceutical agent, the experimental control, the spraying time and method, the sampling and the measurement index were all consistent with those of example 1.
4. Test results
In the experiment of the present example 2020, after 3 single-dose bactericides and 2 binary compound bactericides were treated with 'hua zhe you 261', there was no obvious effect on the thousand-grain weight and the setting rate, and there was no significant difference between each treatment and the control. As the disease incidence of the field district is lighter and the disease grain rate of the field district is not high as a whole, the disease grain rate of 2 binary compound bactericides of hexaconazole-mancozeb (1-1) and azoxystrobin-mancozeb (1-2) treated is the lowest, which are respectively 0.95 percent and 0.86 percent, which are obviously lower than that of loving seedlings (CK-1) and clear water (CK-2), and are also obviously lower than that of 3 single-dosage bactericides, thus having a certain synergistic effect. The relative control of the 2 binary compound bactericides was also significantly better than 3 single-dose bactericides, but did not reach significant levels, with the loving seedling (CK-1) and the clear water (CK-2) as references (Table 7). Therefore, in the experiment in 2020 of this example, the synergy is achieved after the binary combination of 3 single-dosage bactericides, but the synergy is not achieved to a significant level.
The harvested seeds are dried and then subjected to germination tests, and the germination vigor, germination rate, seedling height and root length of the 3 single-dosage bactericides and the 2 binary compound bactericides are not obviously different from those of a clear water (CK-2) control. Demonstrating that these fungicides treated to 'Huazheyou 261' F 1 The vigor and vigor of the seed generation were not adversely affected (Table 8).
Table 7 effect of the chemical treatment on controlling Rice grain smut of' Huazheu 261
TABLE 8 influence of the treatment with the agent on the germination index of seeds of' Huazheyou 261
In the test of 2021 in this example, after the treatment of the ternary complex fungicide, the disease particle rate of the rice particle black powder disease is obviously lower than that of 2 binary complex fungicides and 2 controls, the relative CK-2 control effect and the relative CK-1 control effect respectively reach 71.83% and 27.86%, and are obviously better than that of 2 binary complex fungicides of hexaconazole-mancozeb (1-1) and azoxystrobin-mancozeb (1-2), so that the ternary complex fungicide of the invention has obvious synergistic effect compared with the binary complex fungicide, and is also obviously better than that of the existing field common medicament loving seedling (CK-1). The test results also show that the ternary complex fungicide of the invention has no obvious influence on thousand seed weight and seed setting rate of hybrid rice seeds (Table 9).
The germination test is carried out after the seeds are dried, and the results show that the germination vigor, germination rate, seedling root length and seedling height of the seeds after the ternary complex bactericide treatment are not significantly different from those of the seeds after the clear water treatment (CK-2), which indicates that the ternary complex bactericide has no obvious influence on the viability and vitality of the seeds (Table 10).
Table 9 effects of ternary combination Bactericide on thousand seed weight, seed setting rate, disease seed rate and control efficiency of Zheyou No. 8 in hybrid rice
Influence of ternary complex bactericides in Table 10 on seed germination index after harvesting Zheyou No. 8 in hybrid Rice
Claims (6)
1. A ternary compound bactericide for preventing and treating rice grain smut in hybrid rice seed production fields is characterized by taking water as a solvent and containing 740-750 mg.L of mancozeb -1 190-210 mg.L of hexaconazole -1 240-250 mg.L of azoxystrobin -1 。
2. The ternary complex bactericide for preventing and treating rice grain smut in hybrid rice seed production fields according to claim 1, wherein the ternary complex bactericide contains mancozeb 747 mg.L -1 Hexaconazole 200 mg.L -1 Azoxystrobin 250mg L -1 。
3. The use of a ternary complex fungicide according to any one of claims 1-2 for controlling rice grain smut in hybrid rice seed fields.
4. A method for preventing and controlling rice grain smut in hybrid rice seed production fields, which is characterized by comprising the following steps: applying the ternary complex fungicide according to any one of claims 1-2 to plants twice;
first application: uniformly spraying the plant seed layer on the parent plant seed layer before heading the parent 2 d; second application: uniformly spraying on the scion layer of the female parent plant in the full-period of female parent scion.
5. The method of claim 4, further characterized by a two-application interval of 5 to 7d; the heading full period is a period in which the heading rate reaches 45% -55%.
6. The method according to claim 4, further characterized in that the spraying amount of the ternary complex bactericide is 30-40L per mu -1 。
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CN103598188A (en) * | 2013-08-21 | 2014-02-26 | 陕西康禾立丰生物科技药业有限公司 | Azoxystrobin-containing pesticide composition |
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CN103598188A (en) * | 2013-08-21 | 2014-02-26 | 陕西康禾立丰生物科技药业有限公司 | Azoxystrobin-containing pesticide composition |
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