CN114522232A

CN114522232A - Skin wound healing promoting formula and emulsion

Info

Publication number: CN114522232A
Application number: CN202210121264.7A
Authority: CN
Inventors: 刘添夫; 陈成
Original assignee: Suzhou Nmt Biotech Co ltd
Current assignee: Suzhou Nmt Biotech Co ltd
Priority date: 2022-02-09
Filing date: 2022-02-09
Publication date: 2022-05-24

Abstract

The invention relates to a formula for promoting skin wound healing, which comprises the following components in percentage: 1 to 5 percent of 5-aminolevulinic acid, 0.1 to 0.5 percent of epidermal growth factor, 0.5 to 1 percent of L-hydroxyproline, 0.3 to 1.5 percent of palmitoyl tripeptide-5, 0.3 to 1 percent of ectoin and 0.5 to 1.2 percent of shea butter. According to the invention, 5-ALA, four components for repairing damaged skin and one component for moistening skin are added from external sources, so that the energy of damaged skin cells can be increased, and the quick healing of the damaged skin is facilitated.

Description

Skin wound healing promoting formula and emulsion

Technical Field

The invention relates to a biological medicine technology, in particular to a biological medicine technology used in the field of skin rehabilitation, and specifically shows a formula and an emulsion for promoting skin wound healing.

Background

Whether the skin is damaged due to reasons in work and life, or the skin wound is caused by surgical operation, the healing speed and degree of the damaged skin are very important, and the exposure of the wound can increase the infection probability and risk the wound to be enlarged.

5-aminolevulinic acid is a substance naturally existing in a human body, and the addition of 5-aminolevulinic acid by an external source to supplement endogenous active substances in the human body can also be an effective way for restoring the growth and healing of the wound skin. The 5-aminolevulinic acid can be further accelerated in recovery speed under red light irradiation.

Therefore, there is a need to provide a skin wound healing promoting formulation, an emulsion, to achieve the above objectives.

Disclosure of Invention

The invention aims to provide a formula for promoting skin wound healing.

The technical scheme is as follows:

a skin wound healing promoting formulation comprising, in percent:

1 to 5 percent of 5-aminolevulinic acid, 0.1 to 0.5 percent of epidermal growth factor, 0.5 to 1 percent of L-hydroxyproline, 0.3 to 1.5 percent of palmitoyl tripeptide-5, 0.3 to 1 percent of ectoin and 0.5 to 1.2 percent of shea butter.

Furthermore, 3% -5% of 5-aminolevulinic acid is a substance existing in a human body, is a prefix compound of tetrahydropyrrole substances, is an essential substance for the human body to synthesize heme, vitamin B12 and the like, and the 5-aminolevulinic acid participates in the generation process of adenosine triphosphate in the human body, can increase the energy of all cells, promotes the metabolism of the cells, and has the effects of improving the recovery speed and keeping the skin flatness on various wound skins; the 5-aminolevulinic acid can promote the water production capacity of the skin in the process of participating in energy generation, can keep the skin moist and has a promoting effect on the recovery of skin wounds; under the condition of red light irradiation, the auxiliary 5-aminolevulinic acid can enhance the metabolism speed of cells faster.

Furthermore, 0.1 to 0.5 percent of epidermal growth factor which is heat-resistant single-chain low-molecular polypeptide consisting of 53 amino acid residues has extremely strong biological effect, can promote the proliferation, growth and differentiation of skin cells, promote wound healing and tissue repair, improve the microenvironment of cell life, and promote the regeneration of damaged nerves and the repair of blood vessels, muscles, skin and the like; the epidermal growth factor can improve skin microcirculation, promote metabolism, proliferation and growth of fibroblast and epidermal cell, promote formation of intercellular matrix, accelerate metabolism and renewal of cell, rapidly metabolize aged cutin, and make skin tender, body-building and elastic.

Furthermore, the composition also comprises 0.5% -1% of L-hydroxyproline, wherein the L-hydroxyproline is a natural component of animal structural proteins (such as collagen and elastin), the collagen is a main component forming collagen fibers in connective tissues, and the exogenous addition of the L-hydroxyproline can promote the repair of wound skin and restore the elasticity of the skin.

Further, 0.3-1.5% of palmitoyl tripeptide-5 is included, and the palmitoyl tripeptide-5 can promote the growth of skin cells, inhibit oxygen free radicals and hydroxyl free radicals, promote the synthesis of matrix proteins, particularly collagen, and increase the production of elastin, hyaluronic acid, glycosaminoglycan and fibronectin, so that the skin is more elastic.

Furthermore, the skin care product also comprises 0.3% -1% of ectoin, which can improve the immune protection capability of skin cells and the self-repairing capability of the skin cells, has obvious effect on various injuries of skin tissues and can restore the damaged skin to be smooth and clean.

Furthermore, the skin care product also comprises 0.5-1.2% of shea butter which is an effective component extract of a natural plant shea butter, and the shea butter is relatively close to each index of sebum secretion oil of a human body, contains rich non-saponification components, is easy to absorb by the human body, can prevent drying and cracking, and further recovers and maintains the natural elasticity of the skin.

The invention also aims to provide skin wound healing promotion emulsion which is prepared based on one of the purposes of skin wound healing promotion formula.

Compared with the prior art, the external 5-ALA and the four components for repairing the damaged skin and the one component for moistening the skin are added, so that the energy of damaged skin cells can be increased, and the quick healing of the damaged skin is facilitated.

Drawings

FIG. 1 is a table of the skin irritation test records of the skin wound healing promoting emulsion of example 2 of the present invention.

FIG. 2 is a table of the test records of the speed of accelerating wound healing of skin wound healing lotion according to example 3 of the present invention.

Fig. 3 is a table of test records of the recovery of damaged skin flatness by skin wound healing promoting lotion of example 4 of the present invention.

Detailed Description

Example 1:

this example shows a skin wound healing promoting emulsion comprising, in percent:

1 to 5 percent of 5-aminolevulinic acid, 0.1 to 0.5 percent of epidermal growth factor, 0.5 to 1 percent of L-hydroxyproline, 0.3 to 1.5 percent of palmitoyl tripeptide-5, 0.3 to 1 percent of ectoin, 0.5 to 1.2 percent of shea butter, 3 to 5 percent of glycerol, 3 to 5 percent of butanediol, 0.5 to 1.2 percent of cetostearyl alcohol, 0.3 to 0.5 percent of sucrose stearate, 0.3 to 0.5 percent of squalane, 0.1 to 0.3 percent of potassium cetyl phosphate, 1.5 to 2 percent of caprylic capric triglyceride, 0.5 to 1 percent of sodium polyacrylate and a solvent.

The specific content is not precisely limited in this embodiment, and the list is only one showing manner of the present solution.

5-aminolevulinic acid is an important amino acid existing in mitochondria in cells, is an important component for promoting energy production, and is an effective substance for accelerating metabolism;

the method comprises 1% -5% of 5-aminolevulinic acid, can add the 5-aminolevulinic acid from an external source, and the 5-aminolevulinic acid can directly influence the generation of energy and the efficiency of metabolism, thereby forming a method for supplementing endogenous substances of a human body from an external source and promoting the substances to be synchronously supplemented to increase the healing speed of damaged skin.

The epidermal growth factor is a heat-resistant single-chain low-molecular polypeptide consisting of 53 amino acid residues, has extremely strong biological effect, can promote the proliferation, growth and differentiation of skin cells, promote wound healing and tissue repair, improve the microenvironment of cell life, and promote the regeneration of damaged nerves and the repair of blood vessels, muscles, skin and the like; the epidermal growth factor can improve skin microcirculation, promote metabolism, proliferation and growth of fibroblast and epidermal cell, promote formation of intercellular matrix, accelerate metabolism and renewal of cell, rapidly metabolize aged keratin, and make skin tender, body-building and elastic. The exogenous addition of the epidermal growth factor can improve the differentiation capability of cells at the damaged skin and achieve the effect of promoting wound recovery.

Comprises 0.5% -1% of L-hydroxyproline, which is a natural component of animal structural proteins (such as collagen and elastin), collagen being a main component constituting collagen fibers in connective tissues. The exogenous addition of L-hydroxyproline can promote the repair of wound skin and restore the elasticity of the skin.

Comprises 0.3-1.5% of palmitoyl tripeptide-5, wherein palmitoyl tripeptide-5 can promote skin cell growth, inhibit oxygen free radicals and hydroxyl free radicals, promote the synthesis of matrix protein, especially collagen, and increase the production of elastin, hyaluronic acid, glycosaminoglycan and fibronectin. The effect of restoring the elasticity of the skin can be achieved by adding the palmitoyl tripeptide-5 from an external source.

The ectoin can improve the immune protection capability of skin cells and the self-repairing capability of the skin cells, and has obvious effect on various injuries of skin tissues. The exogenesis addition of ectoin can achieve the effect of enabling the damaged skin to be smooth and clean.

Comprises 0.5-1.2% of shea butter, which is an effective component extract of a natural plant shea butter, has indexes close to each index of sebum secretion oil of a human body, contains rich non-saponification components, is easy to be absorbed by the human body, can prevent drying and cracking, and further recovers and maintains the natural elasticity of skin.

The skin care product also comprises 3% -5% of glycerin which is alcohol, has strong absorption capacity, can moisturize and moisturize the skin, and has strong protection effect on slightly damaged skin.

Also comprises 3 to 5 percent of butanediol, and the butanediol is often used as a humectant and a solvent in cosmetics. In the aspect of moisture preservation, as the butanediol is a micromolecule moisture preservation component, the water grabbing proportion is small, and meanwhile, the butanediol antibacterial moisture-preserving tea has a certain antibacterial effect.

Also comprises 0.5 to 1.2 percent of cetearyl alcohol which is often used as an emollient, a thickening agent, a humectant and an emulsifier, is suitable for various cosmetics, is used as a substrate, and is particularly suitable for cream and emulsion. Has effects of filling the gaps of skin stratum corneum fragments, smoothing skin texture, softening skin, and moisturizing skin.

Also comprises 0.3 to 0.5 percent of sucrose stearate, and the sucrose polystearate is mainly used as an emulsifier, a surfactant and a softening agent in cosmetics and skin care products.

And 0.3 to 0.5 percent of squalane, wherein the squalane has the main effects of moistening and moisturizing, delaying skin aging and repairing epidermis and cells in cosmetics and skin care products.

Also comprises 0.1-0.3% of potassium cetyl phosphate, wherein the potassium cetyl phosphate is mainly used as a surfactant and an emulsifier in cosmetics and skin care products.

The cosmetic also comprises 1.5-2% of caprylic/capric triglyceride, wherein the caprylic/capric triglyceride is high-purity grease formed by esterifying caprylic/capric acid and glycerol, is excellent moisturizing grease, has good spreadability, enables the skin to have a smooth but not greasy feeling, is easy to be absorbed by the skin, plays a good role in the uniform and fine cosmetics, and can lubricate and luster the skin, and the caprylic/capric triglyceride can be used as a base material of a moisturizing factor, a stabilizer of the cosmetics, an antifreezing agent and a homogenizing agent.

Also comprises 0.5 to 1 percent of sodium polyacrylate which is mainly used as an emulsifier and a stabilizer in cosmetics and skin care products.

The solvent is composed of clear water.

The preparation procedure is as follows:

1) adding 5-aminolevulinic acid, epidermal growth factor L-hydroxyproline, palmitoyl tripeptide-5, ectoin, glycerol and butanediol into clear water, controlling the temperature at 60 ℃, and stirring for 20min until all components are dissolved for later use;

2) mixing Shea butter, cetearyl alcohol, sucrose stearate, squalane, potassium cetyl phosphate, and caprylic/capric triglyceride, heating to 65 deg.C, dissolving completely, adding sodium polyacrylate, and stirring for 30 min;

3) pouring the solution obtained in the first step into the oil phase obtained in the second step, and homogenizing for 5min by using a homogenizer.

Example 2:

5 adults with normal skin were selected, and the skin to be tested was cleaned 30min before the test.

The skin wound healing promoting emulsion of example 1 was used as a test sample, about 0.5mL (g) was applied directly to one side of the skin, then covered with two layers of gauze (2.5 cm. times.2.5 cm) and a layer of cellophane or the like, and then secured with a non-irritating adhesive tape and bandage, and the other side of the skin was used as a control. Observing the skin reaction of the applied part at 1, 24, 48 and 72 hours after the test sample is applied, wherein the test result is shown in figure 1;

referring to fig. 1:

erythema is formed in 1h after 5 test volunteers smear samples, the erythema is eliminated, the irritation is stimulated by adding an exogenous stimulus, the irritation is small, the irritation disappears after 1h, and no edema is formed in 5 volunteers.

By comparison, the skin wound healing promoting lotion of example 1 is less irritating to the skin and does not cause further damage to the already damaged skin.

Example 3:

15 adult males with similar skin damage degree at arm and wound length of 10cm were selected and divided into three groups, one group was a treatment group and the other two groups were a control group.

Control group a did not use any repair damaged skin product;

control group B used conventional damaged skin repair products, applied 3 times daily;

the treatment group used a skin wound healing promoting lotion containing the formulation of the present invention 3 times a day;

respectively irradiating three groups of people with red light, observing and recording the time required by the wounds of three groups of volunteers to completely heal, and testing results are shown in figure 2;

referring to fig. 2:

control group a wounds healed completely on day 7;

control group a wounds healed completely on day 5;

the treatment group wounds healed completely on day 3;

as can be seen by comparison, the skin wound healing promoting emulsion of example 1 can significantly accelerate the healing rate of damaged skin.

Example 4:

15 adult males with similar skin damage degree and wound length of 5cm at arm were selected and divided into three groups, one group was a treatment group and the other two groups were a control group.

Control group a did not use any repair damaged skin product;

respectively irradiating three groups of people with red light, recording the flatness and the degree of the scars of the healed wounds by a detector after the wounds of the three groups of volunteers are completely healed, and testing results are shown in figure 3;

referring to fig. 3:

the skin flatness of the healed wound of the control group A is 36%, and the proportion of the scars is 78%;

the skin flatness of the healed wound of the control group B was 78%, and the proportion of the scars was 16%;

the skin flatness of the healed wound of the treatment group is 91 percent, and the proportion of the scars is 7 percent;

by comparison, the skin wound healing promoting emulsion of example 1 can increase the flatness of the healed damaged skin and reduce the formation of skin spots.

What has been described above are merely some embodiments of the present invention. It will be apparent to those skilled in the art that various changes and modifications can be made without departing from the inventive concept thereof, and these changes and modifications can be made without departing from the spirit and scope of the invention.

Claims

1. A formulation for promoting healing of a skin wound, comprising: comprises the following components in percentage:

1 to 5 percent of 5-aminolevulinic acid, 0.1 to 0.5 percent of epidermal growth factor, 0.5 to 1 percent of L-hydroxyproline, 0.3 to 1.5 percent of palmitoyl tripeptide-5, 0.3 to 1 percent of ectoin and 0.5 to 1.2 percent of shea butter, wherein the epidermal growth factor is a heat-resistant single-chain low-molecular polypeptide consisting of 53 amino acid residues.

2. The formulation for promoting healing of a skin wound according to claim 1, wherein: comprises 1 to 5 percent of 5-aminolevulinic acid, wherein the 5-aminolevulinic acid is a substance existing in a human body, is a prefix compound of tetrahydropyrrole substances, and is an essential substance for the human body to synthesize heme, vitamin B12 and the like; the 5-aminolevulinic acid participates in the production process of adenosine triphosphate in a human body, can increase the energy of all cells, promotes the metabolism of the cells, and has the effects of improving the recovery speed and keeping the skin flatness on various wound skins; the 5-aminolevulinic acid can promote the water producing capacity of the skin in the process of participating in energy generation, can keep the skin moist and has a promoting effect on the recovery of skin wounds.

3. The formulation for promoting healing of a skin wound according to claim 2, wherein: the skin-care product comprises 0.1 to 0.5 percent of epidermal growth factor, and the epidermal growth factor has extremely strong biological effect, can promote the proliferation, growth and differentiation of skin cells, promote wound healing and tissue repair, improve the microenvironment of cell life, and promote the regeneration of damaged nerves and the repair of blood vessels, muscles, skin and the like; the epidermal growth factor can improve skin microcirculation, promote metabolism, proliferation and growth of fibroblast and epidermal cell, promote formation of intercellular matrix, accelerate metabolism and renewal of cell, rapidly metabolize aged keratin, and make skin tender, body-building and elastic.

4. The formulation for promoting healing of a skin wound according to claim 2, wherein: comprises 0.5% -1% of L-hydroxyproline, wherein the L-hydroxyproline is a natural component of animal structural proteins (such as collagen and elastin), the collagen is a main component forming collagen fibers in connective tissues, and the exogenous addition of the L-hydroxyproline can promote the repair of wound skin and restore the elasticity of the skin.

5. The formulation for promoting healing of a skin wound according to claim 2, wherein: comprises 0.3-1.5% of palmitoyl tripeptide-5, wherein palmitoyl tripeptide-5 can promote skin cell growth, inhibit oxygen free radicals and hydroxyl free radicals, promote the synthesis of matrix protein, especially collagen, and increase the production of elastin, hyaluronic acid, glycosaminoglycan and fibronectin, so as to make skin more elastic.

6. The formulation for promoting healing of a skin wound according to claim 2, wherein: the ectoin can improve the immune protection capability of skin cells and the self-repairing capability of the skin cells, has obvious effect on various injuries of skin tissues and can ensure that the damaged skin is smooth and clean.

7. The formulation for promoting healing of a skin wound according to claim 2, wherein: comprises 0.5-1.2% of shea butter, which is an effective component extract of a natural plant shea butter, has indexes close to each index of sebum secretion oil of a human body, contains rich non-saponification components, is easy to be absorbed by the human body, can prevent drying and cracking, and further recovers and maintains the natural elasticity of skin.

8. An emulsion for promoting healing of skin wounds, which is prepared based on the skin wound healing promoting formulation of claim 2.