CN114515304A

CN114515304A - Plant composition for preventing and treating and repairing skin sensitivity and preparation method thereof

Info

Publication number: CN114515304A
Application number: CN202210189315.XA
Authority: CN
Inventors: 孟令超
Original assignee: Individual
Current assignee: Individual
Priority date: 2022-02-24
Filing date: 2022-02-24
Publication date: 2022-05-20

Abstract

The invention discloses a plant composition for preventing and treating and repairing skin sensitivity, which comprises the following raw materials in percentage by weight: 0.5-20% of patchouli extract, 0.5-20% of hop extract and 0.5-20% of rosemary extract. The product of the invention can quickly and effectively relieve the discomfort caused by skin sensitivity, such as stabbing pain, pruritus and the like. After long-term use, the skin allergy symptoms such as skin discomfort, erythema index, percutaneous water loss rate, skin hydration and the like can be obviously relieved and improved, and the skin barrier function can be repaired. The product has water-oil amphipathy through scientific solvent proportion and combination, can be dissolved and used in a water phase and an oil phase at a high proportion, and can exert the effect of the product to the maximum extent according to different application products.

Description

Plant composition for preventing and treating and repairing skin sensitivity and preparation method thereof

Technical Field

The invention relates to the field of biological medicines and cosmetics, in particular to a plant composition for preventing and repairing skin sensitivity and a preparation method thereof.

Background

According to epidemiological research, the prevalence rate of sensitive skin of women is as high as 60% -70%, and the prevalence rate of sensitive skin of men is as high as 50% -60%, at present, the skin sensitivity is generally considered not to belong to the category of skin diseases, but to be metastable skin, and the sensitive skin has the characteristics of unclear symptom expression, unclear reason, uncertain relieving mode and the like. However, when skin sensitivity occurs, symptoms such as red and swollen skin, erythema, tightness, abnormal stabbing pain, burning sensation, pruritus and the like are often accompanied, the life and social activities of patients are seriously affected, and great psychological pressure is brought to the patients.

The clinical definition of sensitive skin is a series of characteristic sensations including tightness, abnormal stinging, burning, stinging, pain and itching. Although usually transient and in many cases without visual skin reactions, sensitive skin can affect quality of life. Sometimes, the skin may be reddened, erythematous, dry, desquamated, or accompanied by the expansion of capillaries, and may be affected by external stimuli such as temperature change, ultraviolet rays, and dust, and may occur on the face, scalp, and hands.

There are many factors that induce skin sensitization, which can be divided into extrinsic factors (environment, lifestyle, chemicals) and intrinsic factors (endocrine, genetic inheritance, diseases, physiological cycle, etc.). The environmental factors mainly comprise climate change, air pollution, air humidity, ultraviolet intensity and the like caused by alternate seasons; excessive and inappropriate skin care, irregular work and rest, overnight stay up, unhealthy diet and other lifestyle habits can also promote skin sensitivity; skin sensitivity is also induced by irritant substances contained in products such as daily cosmetics, hair dyes, detergents and the like. For individuals, different ethnicities and different individuals also exhibit differences in skin sensitivity due to genetic differences; endocrine imbalance may also cause skin sensitivity due to weakened skin resistance and enhanced skin immune response; people who have skin diseases such as skin inflammation, psoriasis and acne rosacea and internal organ pathological changes are also prone to develop skin sensitivity. Particularly, people with sensitive skin are prone to have problems of dry and dry skin due to lack of water, redness and pruritus and the like when seasons change. Also inappropriate skin care measures, such as "acid brushing", "skin pouring", abuse of skin care products, use of defective products, etc., can be a contributing factor to skin sensitivity.

In view of the existing various products for preventing, treating and repairing sensitive skin, the method has certain limitations. Therefore, there is a need to develop a safe, effective and highly bioavailable product for preventing and repairing skin allergy, and a new drug or cosmetic choice is provided for skin allergy sufferers.

Disclosure of Invention

The invention aims to provide a plant composition for preventing and repairing sensitive skin, which solves the problems in the prior art.

In order to achieve the purpose, the invention provides the following technical scheme:

a plant composition for preventing and repairing sensitive skin, wherein the plant composition comprises the following raw materials in percentage by weight: 0.5-20% of patchouli extract, 0.5-20% of hop extract and 0.5-20% of rosemary extract.

In some embodiments, the botanical composition further comprises a surfactant and a solvent; the surfactant is one or more of PEG-40 hydrogenated castor oil, coconut oil alcohol polyether-7, polyglycerol-10 laurate, polyglycerol-10 stearate, Tween 80, soybean lecithin or coconut oil alcohol diethanolamide; the solvent is alcohol and/or water; the alcohol is ethanol, glycerol, 1, 2-propylene glycol, 1, 3-propylene glycol, 1, 4-butanediol, 2, 3-butanediol or 1, 3-butanediol. The sum of the weight percentages of the raw materials, the surfactant and the solvent is 100 percent.

In some embodiments, the botanical composition comprises the following raw materials in weight percent: 2-10% of patchouli extract, 2-10% of hop extract and 2-10% of rosemary extract.

In some embodiments, the weight percentage of the surfactant and the solvent is 70% to 90%.

In some embodiments, the plant composition of the present invention comprises the following raw materials in percentage by weight: 5% of patchouli extract, 5% of hop extract and 5% of rosemary extract.

In some embodiments, the plant composition of the present invention comprises the following raw materials in weight percent: 10% of patchouli extract, 5% of hop extract and 5% of rosemary extract.

In some embodiments, the plant composition of the present invention comprises the following raw materials in weight percent: 8% of patchouli extract, 5% of hop extract and 5% of rosemary extract.

The invention also provides a preparation method of the plant composition for preventing and treating and repairing skin sensitivity, which is characterized by comprising the following steps:

1) mixing 0.5-20% of patchouli extract, 0.5-20% of hop extract and 0.5-20% of rosemary extract to obtain a mixture;

2) Adding a surfactant and the balance of solvent into the mixture, heating to 45-60 ℃, refluxing, and stirring until all the components are completely dissolved;

3) filtering with a 0.1-0.5 μm membrane, and cooling the filtrate to room temperature to obtain the plant composition;

wherein said patchouli extract, hops extract, rosemary extract are distilled with steam or supercritical CO₂The preparation method is adopted.

In some embodiments, the patchouli extract is prepared by (steam distillation) as follows:

1) pulverizing stem and leaf of herba Agastaches into powder with pulverizer, and sieving with 20 mesh sieve to obtain raw material powder;

2) adding raw material powder into deionized water with the weight 8-16 times of that of the raw material powder, and soaking for 2-6 hours at normal temperature;

3) after soaking, raising the temperature to about 100 ℃ for distillation for 2-6 hours, and recycling and storing the distillate through a condenser;

4) and standing the distillate for 24-48 hours, and performing oil-water two-phase separation to obtain an oil phase, namely the patchouli extract.

In some embodiments, the patchouli extract is prepared by the following method (supercritical CO)₂Method):

1) pulverizing stem and leaf of herba Agastaches into powder with pulverizer, and sieving with 40 mesh sieve to obtain raw material powder;

2) using supercritical CO ₂Extracting by a method, wherein the extraction pressure is 6.0-20.0 Mpa, the extraction time is 2-5 h, and the extraction temperature is 35-45 ℃;

3) after extraction, the mixture is fed into a separator, and the mixture is separated into gas phase and liquid phase by reducing pressure, wherein the liquid phase (oil phase) is the patchouli extract.

In some embodiments, the hops extract is prepared by (steam distillation) as follows:

1) pulverizing hops into powder by a pulverizer, and sieving with a 20-mesh sieve to obtain raw material powder for later use;

4) and standing the distillate for 24-48 hours, and performing oil-water two-phase separation to obtain an oil phase, namely the hop extract.

In some embodiments, the hops extract is prepared by a process (supercritical CO)₂Method):

1) pulverizing hops into powder by a pulverizer, and sieving with a 40-mesh sieve to obtain raw material powder for later use;

2) using supercritical CO₂Extracting by a method, wherein the extraction pressure is 6.0-20.0 Mpa, the extraction time is 2-5 h, and the extraction temperature is 35-45 ℃;

3) after extraction, the extraction mixture is fed into a separator, and the mixture is separated into a gas phase and a liquid phase (oil phase) through pressure reduction, wherein the liquid phase (oil phase) is the hop extract.

In some embodiments, the rosemary extract is prepared by a method comprising:

1) pulverizing Rosemary flower and leaf into powder with a pulverizer, and sieving with a 20-mesh sieve to obtain raw material powder for later use;

2) adding raw material powder into deionized water 8-16 times of the weight of the raw material powder, and soaking for 2-6 hours at normal temperature;

3) after soaking, raising the temperature to about 100 ℃ for distillation, wherein the distillation time is 2-6 hours, and recovering and storing the distillate through a condenser;

4) and standing the distillate for 24-48 hours, and performing oil-water two-phase separation to obtain an oil phase, namely the rosemary extract.

In some embodiments, the rosemary extract is prepared by a process (supercritical CO)₂Method):

1) pulverizing Rosemary flower and leaf into powder with a pulverizer, and sieving with a 40-mesh sieve to obtain raw material powder for later use;

3) after extraction, the mixture is pumped into a separator, and the mixture is separated into a gas phase and a liquid phase (oil phase) through pressure reduction, wherein the liquid phase (oil phase) is the rosemary extract.

The product of the invention selects traditional plant medicinal materials of patchouli (Pogostemon cablin (Blanco) Benth.), hop (Humulus lupulus Linn.), rosemary (Rosmarinus officinalis), and enriches a plurality of active ingredients of plants by an extraction and purification technology: terpenes, ketones, phenolic compounds, organic acids, alcohols and the like, and then the composition is prepared by scientific proportioning. The product of the invention can quickly and effectively relieve the discomfort caused by skin sensitivity, such as stabbing pain, pruritus and the like. Can activate subcutaneous immune cell population, inhibit macrophage polarization, inhibit expression of inflammatory factor and trend factor, and reduce skin inflammation reaction and expression, such as skin redness, erythema, telangiectasia, etc. Can remarkably relieve and improve skin sensitivity symptoms such as skin discomfort, erythema index, percutaneous water loss rate, skin hydration, and repair skin barrier function after long-term use. The score of the Lactic Acid Sting Test (LAST), a skin sensitivity test, can be greatly reduced by using the product of the invention (0-4 score principle, higher score indicates higher skin sensitivity). The product of the invention is a composition which is derived from plants, safe and effective in preventing, treating and repairing sensitive skin. The product has water-oil amphipathy through scientific solvent proportion and combination, can be dissolved and used in a water phase and an oil phase at a high proportion, and can exert the effect of the product to the maximum extent according to different application products.

Drawings

FIG. 1: example 1 site schematic for anti-irritation and soothing effect testing.

FIG. 2 is a schematic diagram: line graphs reflecting the anti-irritant and soothing effects of example 1.

FIG. 3: bar graph reflecting the inhibitory effect of example 1 on the production of inflammatory factor TNF-alpha by LPS-induced human fibroblasts.

FIG. 4: bar graph reflecting the inhibitory effect of example 1 on the production of the inflammatory factor IL-1 β by LPS-induced human fibroblasts.

FIG. 5: bar graph reflecting the inhibitory effect of example 1 on the production of IL-6, an inflammatory factor induced by LPS in human fibroblasts.

FIG. 6: bar graph reflecting the treatment and repair of skin sensitivity, and reduction of skin erythema area of example 1.

FIG. 7: bar graph reflecting treatment and repair of skin sensitivity, decreasing skin erythema Index (IE) of example 1.

FIG. 8: bar graph reflecting example 1 treatment and repair of skin sensitivity, reducing the transdermal water loss rate (TEWL) of the skin.

FIG. 9: bar chart reflecting treatment and repair of skin sensitivity, promoting skin keratolysis of example 1.

FIG. 10: reaction example 1 bar graph of lactate sting assay (LAST) score for treating and repairing skin sensitivity.

Detailed Description

The technical solution of the present patent will be described in further detail with reference to the following embodiments.

Example 1 plant composition 1

The plant composition formula comprises the following raw materials in percentage by weight: 5g of patchouli extract, 5g of hop extract and 5g of rosemary extract;

30g PEG-40 hydrogenated castor oil, 10g Coco polyether-7, 15g polyglycerol-10 laurate;

20g of 1, 3-butanediol and 10g of deionized water;

the total amount was 100 g.

The preparation method comprises the following steps:

(1) pulverizing stem and leaf of herba Agastaches into powder with pulverizer, and sieving with 20 mesh sieve to obtain raw material powder for use. The raw material powder is added into deionized water with the weight 12 times of the raw material powder, and the mixture is soaked for 3 hours at normal temperature. After soaking, raising the temperature to about 100 ℃ for distillation for 4 hours, recovering and storing the distillate by a condenser, standing for 36 hours, and performing oil-water two-phase separation to obtain an oil phase which is the patchouli extract and weighing 5g for later use;

(2) pulverizing flos Lupuli into powder with a pulverizer, and sieving with 20 mesh sieve to obtain raw material powder for use. The raw material powder is added into deionized water with the weight 12 times of the raw material powder, and the mixture is soaked for 3 hours at normal temperature. After the soaking, the temperature is raised to about 100 ℃ for distillation for 4 hours, and the distillate is recovered and stored by a condenser. Standing the distillate for 36 hr, performing oil-water two-phase separation to obtain oil phase (hop extract), and weighing 5 g;

(3) Pulverizing Rosemary flower and leaf into powder with a pulverizer, and sieving with a 20-mesh sieve to obtain raw material powder for later use. The raw material powder is added into deionized water with the weight 12 times of the raw material powder, and the mixture is soaked for 3 hours at normal temperature. After the soaking, the temperature is raised to about 100 ℃ for distillation for 4 hours, and the distillate is recovered and stored by a condenser. Standing the distillate for 36 hr, performing oil-water two-phase separation to obtain oil phase which is herba Rosmarini officinalis extract, and weighing 5 g;

(4) mixing herba Agastaches extract 5g, flos Lupuli extract 5g, and herba Rosmarini officinalis extract 5 g;

(5) then adding a surfactant and the balance of solvent into the mixed extract according to the total mass percentage of the raw materials: 30g of PEG-40 hydrogenated castor oil, 710 g of coco-alcohol polyether, 15g of polyglycerol-10 laurate, 20g of 1, 3-butanediol and 10g of deionized water, wherein the total amount is 100 g. Heating to 55 deg.C, refluxing, and stirring until all components are completely dissolved;

(6) filtering with 0.2 μm membrane, and cooling the filtrate to room temperature to obtain the plant composition 1.

Example 2 plant composition 2

The plant composition formula comprises the following raw materials in percentage by weight: 10g of patchouli extract, 5g of hop extract and 5g of rosemary extract;

25g PEG-40 hydrogenated castor oil, 8g Coco polyether-7 and 12g polyglycerol-10 laurate;

30g of 1, 4-butanediol and 5g of deionized water;

the total amount was 100 g.

The preparation method comprises the following steps:

(1) pulverizing stem and leaf of herba Agastaches into powder with pulverizer, and sieving with 40 mesh sieve to obtain raw material powder for use. Using supercritical CO₂Extracting at 40 deg.C under 18.0MPa for 3 hr. After extraction, putting the extraction mixture into a separator, separating the mixture into a gas phase and a liquid phase by reducing pressure, wherein the liquid phase (oil phase) is the patchouli extract, and weighing 10g for later use;

(2) pulverizing flos Lupuli into powder with a pulverizer, and sieving with 40 mesh sieve to obtain raw material powder for use. Using supercritical CO₂Extracting at 40 deg.C under 18.0MPa for 3 hr. After extraction, putting the extraction mixture into a separator, reducing pressure to separate the mixture into a gas phase and a liquid phase, wherein the liquid phase (oil phase) is the hop extract, and weighing 5g for later use;

(3) pulverizing Rosemary flower and leaf into powder with a pulverizer, and sieving with a 40-mesh sieve to obtain raw material powder for later use. Using supercritical CO₂Extracting at 40 deg.C under 18.0MPa for 3 hr. After extraction, pumping the extraction mixture into a separator, reducing pressure to separate the mixture into a gas phase and a liquid phase, wherein the liquid phase (oil phase) is the rosemary extract, and weighing 5g for later use;

(4) Mixing herba Agastaches extract 10g, flos Lupuli extract 5g, and herba Rosmarini officinalis extract 5 g;

(5) then adding a surfactant and the balance of solvent into the mixed extract according to the total mass percentage of the raw materials: 25g of PEG-40 hydrogenated castor oil, 78 g of coco-alcohol polyether, 12g of polyglycerol-10 laurate, 30g of 1, 4-butanediol and 5g of deionized water, wherein the total amount is 100 g. Heating to 55 deg.C, refluxing, and stirring until all components are completely dissolved;

(6) filtering with 0.2 μm membrane, and cooling the filtrate to room temperature to obtain plant composition 2.

Example 3 plant composition 3

The plant composition formula comprises the following raw materials in percentage by weight: 8g of patchouli extract, 5g of hop extract and 5g of rosemary extract;

27g PEG-40 hydrogenated castor oil, 10g Coco polyether-7 and 10g polyglycerol-10 laurate;

27g of 2, 3-butanediol and 8g of deionized water;

the total amount was 100 g.

The preparation method comprises the following steps:

(1) pulverizing stem and leaf of herba Agastaches into powder with pulverizer, and sieving with 20 mesh sieve to obtain raw material powder for use. The raw material powder is added into deionized water with the weight 10 times of the raw material powder, and the mixture is soaked for 4 hours at normal temperature. After soaking, raising the temperature to about 100 ℃ for distillation for 5 hours, recovering and storing the distillate by a condenser, standing for 48 hours, and performing oil-water two-phase separation to obtain an oil phase which is the patchouli extract and weighing 8g for later use;

(2) Pulverizing flos Lupuli into powder with a pulverizer, and sieving with 40 mesh sieve to obtain raw material powder for use. Using supercritical CO₂Extracting at 45 deg.C under 16.0MPa for 4 hr. After extraction, putting the extraction mixture into a separator, reducing pressure to separate the mixture into a gas phase and a liquid phase, wherein the liquid phase (oil phase) is the hop extract, and weighing 5g for later use;

(3) pulverizing Rosemary flower and leaf into powder with a pulverizer, and sieving with a 40-mesh sieve to obtain raw material powder for later use. Using supercritical CO₂Extracting at 38 deg.C under 12.0MPa for 2.5 hr. After extraction, pumping the extraction mixture into a separator, reducing pressure to separate the mixture into a gas phase and a liquid phase, wherein the liquid phase (oil phase) is the rosemary extract, and weighing 5g for later use;

(4) mixing herba Agastaches extract 8g, flos Lupuli extract 5g, and herba Rosmarini officinalis extract 5 g;

(5) then adding a surfactant and the balance of solvent into the mixed extract according to the total mass percentage of the raw materials: 27g of PEG-40 hydrogenated castor oil, 710 g of coco polyether, 10g of polyglycerol-10 laurate, 27g of 2, 3-butanediol and 8g of deionized water, wherein the total amount is 100 g. Heating to 60 deg.C, refluxing, and stirring until all components are completely dissolved;

(6) Filtering with 0.5 μm membrane, and cooling the filtrate to room temperature to obtain the plant composition 3.

Preparation of comparative example 1

30g of PEG-40 hydrogenated castor oil, 710 g of coco polyether, and 15g of polyglycerol-10 laurate;

20g of 1, 2-propylene glycol and 10g of deionized water;

the total amount was 100 g.

A botanical composition comparative example 1 was prepared essentially following the procedure and conditions of example 1.

Preparation of comparative example 2

tween 8030g, coconut oil alcohol polyether-710 g and polyglycerol-10 laurate 15 g;

20g of 1, 3-butanediol and 10g of deionized water;

the total amount was 100 g.

A comparative botanical composition example 2 was prepared essentially following the procedure and conditions of example 1.

Preparation of comparative example 3

30g of PEG-40 hydrogenated castor oil, 10g of coconut oil alcohol diethanolamide and 15g of polyglycerol-10 laurate;

20g of 1, 3-butanediol and 10g of deionized water;

the total amount was 100 g.

A comparative botanical composition example 3 was prepared essentially following the procedure and conditions of example 1.

Preparation of comparative example 4

30g of PEG-40 hydrogenated castor oil, 710 g of cocoalcohol polyether, 10g of polyglycerol-10 stearate and 5g of soybean lecithin; 20g of 1, 3-butanediol and 10g of deionized water;

the total amount was 100 g.

A comparative botanical composition example 4 was prepared essentially following the procedure and conditions of example 1.

In order to verify the effect of the product of the invention in preventing and treating and repairing skin sensitivity, the following tests were carried out:

first, anti-irritation and soothing Effect test (in vivo test)

Sensitive skin responds relatively violently to external stimuli, and for example, uncomfortable feelings such as stabbing pain, pruritus and the like can be generated after the sensitive skin is stimulated by chemical substances. Therefore, the method reduces the reaction expression of skin to external stimulation, relieves and reduces the uncomfortable feeling of the skin, and is an important link for treating skin sensitivity.

The test is an in vivo test experiment, and 18 subjects are recruited, wherein 10 women and 8 men are 24-65 years old. The test method is that deionized water is smeared on one side of the opposite side of the nasolabial sulcus of the face of a subject to be tested to be used as a blank Control (Control), and 1% of the product of the invention in the embodiment 1 is smeared on the other side of the face of the subject for 15 minutes, and then 100ppm of capsaicin solution (capsaicin is an activator of TRPV1 receptors and can mediate discomfort such as skin stabbing pain, burning and the like) is smeared on the two sides respectively to stimulate the skin to cause stabbing pain and burning sensation. The evaluation of the subjects for the degree of sting was recorded every 1 minute after application, with 0-4 as the scoring interval. The test site schematic, scoring principle and scoring result are shown in fig. 1 and fig. 2.

The experimental result shows that the capsaicin can obviously stimulate the skin to generate uncomfortable feelings (stabbing pain and burning feelings), when 1% of the product of the embodiment 1 of the invention is used for treatment, the uncomfortable feelings of the skin caused by the capsaicin stimulation of a subject can be greatly reduced, and the overall uncomfortable feeling is reduced by 56% through statistics of the scoring result of the subject.

Anti-inflammatory action (in vitro test)

A large number of scientific reports prove that the generation and expression of inflammation are directly and indirectly related to the formation and the occurrence of skin sensitivity, and inflammatory factors can mediate discomfort (such as stabbing pain and pruritus), redness, abnormal skin barrier function and the like of the skin. Therefore, the prevention of inflammation and the inhibition of inflammation expression play important roles in preventing and repairing skin sensitivity. Among them, TNF-alpha, IL-1 beta and IL-6 are key inflammatory expression factors.

In this test, LPS (Lipopolysaccharide, used at a concentration of 1. mu.g/ml) was used to induce an inflammatory reaction in human fibroblasts (HSF), the product of example 1 of the present invention (used at a concentration of 5. mu.M, 10. mu.M, 20. mu.M, respectively) was used for treatment, and finally the expression of the inflammatory factors TNF-. alpha., IL-1. beta., and IL-6 were measured and evaluated by ELISA (Enzyme linked immunosorbent assay). The test results are shown in fig. 3, 4 and 5.

From the test results, when LPS is used for inducing human fibroblasts (HSF), the expressions of TNF-alpha, IL-1 beta and IL-6 are remarkably increased compared with a blank Control group (Control), which shows that the LPS induces inflammation and the expressions, and when the LPS is used for inducing and the products of the invention in the embodiment 1 with the concentrations of 5 MuM, 10 MuM and 20 MuM are respectively added for treatment, the expressions of TNF-alpha, IL-1 beta and IL-6 are remarkably reduced and are in a concentration dependence relationship, which shows that the products of the invention have remarkable inhibition effect on the expression of inflammatory factors induced by the LPS.

Third, long-acting prevention and cure and repair skin sensitivity (in vivo test)

Patients with sensitive skin generally have symptoms and manifestations of reddening of the skin, increased transepidermal water loss (TEWL), decreased hydration of the stratum corneum, and sensitivity of the skin to external stimuli. A common method for testing skin sensitivity is the Lactic Acid Sting Test (LAST), which generally uses a score of 0-4, with higher scores indicating a more intense response of the skin to the stimulus and a higher skin sensitivity.

The test is a human in-vivo test experiment, 18 subjects are recruited together, all the subjects are female, the skin is between 25 and 40 years old, the skin is sensitive to a certain degree and is reddish, and the lactic acid test score is more than or equal to 3. Test methods test cream (containing 1% of the product of example 1 according to the invention) and placebo cream (without any product according to the invention) were applied twice a day, in the morning and at night, on the left and right sides of the face. The total time is 10 days, any other skin care products or medicinal products and the like are not allowed to be used in the period, and if the skin care products or the medicinal products occur, the skin care products or the medicinal products are immediately excluded from testing. Subjects were tested for Erythema area, Erythema index (EI, higher index indicates more severe facial Erythema), transdermal water loss rate (TEWL), keratolysis, and lactate sting test (LAST) scores on

days

0, 5, and 10, respectively. The product of example 1 of the present invention was finally evaluated for its therapeutic and ameliorating effects on sensitive skin. The test results are shown in fig. 6, 7, 8, 9 and 10.

As can be seen from the experimental results, after the test cream containing 1% of the product of example 1 of the present invention was used, the area of erythema and the erythema Index (IE) of the face of the subject were significantly reduced, while there was no significant change in the placebo group, which demonstrates that the test cream containing 1% of the product of example 1 of the present invention can significantly reduce the skin redness caused by sensitivity or inflammation; the test cream group containing 1% of the product of example 1 of the invention showed a significant improvement in TEWL, a significant increase in keratohydration, and a significant reduction in the lactate stinging assay score. The placebo group has no obvious change, and the product of the invention in the example 1 can obviously improve the symptoms related to the skin sensitivity and achieve the effects of preventing, treating and repairing the skin sensitivity.

Fourthly, testing the stability of the product water solution

In the market application process of the product, the product needs to be added into a final product according to a certain proportion, so that the solubility and the stability of the product in an aqueous phase system are particularly important. The product with excellent solubility and stability can exert greater efficacy and bioavailability of the product, and provides a wider, convenient and reliable application mode for the formula development of products in downstream markets.

In this test, example 1, example 2, example 3, comparative example 1, comparative example 2, comparative example 3, and comparative example 4 were used, 100g of each 1% aqueous solution was prepared with deionized water (i.e., 1g of the product was dissolved in 99g of deionized water), and the resultant solution was sufficiently dissolved by ultrasonic waves and sealed in a transparent PE bottle as a sample to be tested, followed by a stability test. The stability test method comprises the following steps: the samples to be tested were left at-20 ℃ for 48 hours and then at 45 ℃ for another 48 hours, alternating, for a total duration of 6 weeks (42 days). In the whole experimental process, the appearance change of each test sample is observed, the clearer appearance sample shows better stability, and conversely, if the product with the phenomena of turbidity, layering, precipitation and the like appears, the relative stability is poor, and the specific test conditions are detailed in the following table 1.

Table 1: examples and comparative examples 1% aqueous solution stability test results

Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes, substitutions and alterations can be made herein without departing from the spirit and scope of the invention as defined by the appended claims.

Claims

1. The plant composition is characterized by comprising the following raw materials in percentage by weight: 0.5-20% of patchouli extract, 0.5-20% of hop extract and 0.5-20% of rosemary extract.

2. The plant composition of claim 1, further comprising a surfactant and a solvent; the surfactant is one or more of PEG-40 hydrogenated castor oil, coconut oil alcohol polyether-7, polyglycerol-10 laurate, polyglycerol-10 stearate, Tween 80, soybean lecithin or coconut oil alcohol diethanolamide; the solvent is alcohol and/or water; the alcohol is ethanol, glycerol, 1, 2-propylene glycol, 1, 3-propylene glycol, 1, 4-butanediol, 2, 3-butanediol or 1, 3-butanediol.

3. The botanical composition of claim 2, wherein the surfactant is PEG-40 hydrogenated castor oil, cocoeth-7, and polyglycerol-10 laurate.

4. The botanical composition of claim 2 or 3, wherein the alcohol is 1, 4-butanediol, 2, 3-butanediol or 1, 3-butanediol.

5. The plant composition according to any one of claims 1 to 4, wherein the plant composition comprises the following raw materials in percentage by weight: 2-10% of patchouli extract, 2-10% of hop extract and 2-10% of rosemary extract.

6. The plant composition according to claim 5, wherein the plant composition comprises the following raw materials in percentage by weight: 5% of patchouli extract, 5% of hop extract and 5% of rosemary extract.

7. The plant composition according to claim 5, wherein the plant composition comprises the following raw materials in percentage by weight: 10% of patchouli extract, 5% of hop extract and 5% of rosemary extract.

8. The plant composition according to claim 5, wherein the plant composition comprises the following raw materials in percentage by weight: 8% of patchouli extract, 5% of hop extract and 5% of rosemary extract.

9. A method for preparing a plant composition according to any one of claims 1 to 8, comprising the steps of:

2) adding a surfactant and a solvent into the mixture, heating to 45-60 ℃, refluxing, and stirring until all components are completely dissolved;

10. Use of a plant composition according to any one of claims 1 to 8, comprising: preventing and treating skin allergy, repairing skin allergy, relieving and tranquilizing skin, and/or repairing skin barrier.