CN114480301A

CN114480301A - Vaccine for preventing or treating avian viral infection

Info

Publication number: CN114480301A
Application number: CN202210072761.2A
Authority: CN
Inventors: 刘凤丽; 曹艳欣; 苏敬良
Original assignee: China Agricultural University
Current assignee: China Agricultural University
Priority date: 2022-01-21
Filing date: 2022-01-21
Publication date: 2022-05-13
Anticipated expiration: 2042-01-21
Also published as: CN114480301B

Abstract

The application provides a duck plague virus (also called duck enteritis virus) low virulent strain DEV SD70 (microorganism preservation number CGMCC 45056), a recombinant virus based on the low virulent strain DEV SD70, a virus strain, a vaccine and a recombinant virus for preventing and treating waterfowl related epidemic diseases.

Description

Vaccine for preventing or treating avian viral infection

Technical Field

The present application relates to the field of biomedicine, and more particularly to duck herpes virus type 1 (anated herpesvirus 1) virus strains, vaccines for preventing or treating avian viral infections comprising the same, and recombinant viruses based on the same.

Background

Duck viral enteritis (duck viral enteritis), also known as duck plague (duck platue), is an infectious disease caused by duck plague virus (duck platue virus) or duck viral enteritis virus (duck viral enteritis virus). The infection mainly occurs in members of anseriformes anatidae, such as ducks, geese, ducks and the like, is an acute and highly lethal disease, seriously harms the development of water and poultry breeding, and is mainly characterized by mucosal vascular injury bleeding and organ hemorrhage necrosis. The immunization vaccine is one of effective measures for preventing duck viral enteritis. The vaccine for preventing and treating duck plague at present is a C-KCE attenuated strain which is obtained by transmitting 8 generations of duck embryos and then continuously transmitting 20 generations of chicken embryos to cause attenuated virulence (edited by Haimai et al, 2 nd edition of veterinary biological production, China agricultural Press, 2018). In recent years, the genome sequence of the attenuated vaccine strain has been reported, but the genetic background of the parental strain is unclear, so that the molecular genetic mechanism of attenuated virulence to the strain is not clear. Understanding and mastering the genetic evolution of duck enteritis virus virulence attenuation, breeding attenuated strains with good immunogenicity has important theoretical and practical significance for improving the safety of vaccine use and developing duck enteritis virus recombinant live vector vaccines.

Currently, there is an urgent need for a vaccine that is safe and effective for preventing or treating avian viral infections.

Disclosure of Invention

One aspect of the present application provides a duck herpesvirus type 1 (anatated herpesvirus 1) virus strain belonging to the family herpesviridae, subfamily alphaherpesviridae and genus marek's virus, wherein the virus strain has the genomic sequence shown in SEQ ID No. 1 or a genomic sequence with at least 90% identity to the sequence shown in SEQ ID No. 1.

According to an embodiment of the present application, the genome of said virus strain comprises the sequence UL1 shown in SEQ ID NO. 2.

According to an exemplary embodiment of the present application, the virus strain is DEV SD70 with the preservation number CGMCC 45056.

According to an exemplary embodiment of the present application, the virus strain DEV SD70 has the genomic sequence shown in SEQ ID NO 1.

According to an exemplary embodiment of the present application, the virus strain DEV SD70 has the sequence UL1 as shown in SEQ ID NO: 2.

According to an exemplary embodiment of the present application, the genomic sequence of 154685bp of the strain DEV SD70 has the following mutations with respect to the genomic sequence of 160663bp of the strain DEV SD 04:

2311 deletion of nucleotide 5373 (3063nt),

146179-148673 (2495nt) nucleotide is replaced by 388nt of the coding frame SORFx,

123708 together with the deletion of nucleotide 123907 (200nt),

126731 and 127138 (408nt) nucleotide deletion,

157484-deletion of nucleotide at position 157891 (408nt),

131060-

153561 153562 insert of nucleotide (104 nt).

A further aspect of the present application proposes a vaccine for the prevention or treatment of an avian viral infection comprising a therapeutically effective amount of the virus strain DEV SD70 having accession number CGMCC 45056.

According to an embodiment of the application, the avian viral infection is a disease caused by duck plague virus. In an exemplary embodiment, the avian viral infection is duck viral enteritis.

According to an embodiment of the present application, said virus strain DEV SD70 is a live, attenuated live or inactivated virus strain. In an exemplary embodiment, the virus strain DEV SD70 is a live virus strain.

According to an embodiment of the present application, the viral strain DEV SD70 in the vaccine is at 1 × 10²TCID₅₀/ml or TCID₅₀G to 1X 10⁸TCID₅₀/ml or TCID₅₀In the dosage of/g. In an exemplary embodiment, said viral strain DEV SD70 in said vaccine is at 5 x 10⁴TCID₅₀/ml or TCID₅₀G to 2X 10⁶TCID₅₀/ml or TCID₅₀In the dosage of/g.

According to an embodiment of the application, the vaccine further comprises an adjuvant.

In a further aspect of the present application is presented the use of the virus strain DEV SD70 having accession number CGMCC45056 for the manufacture of a medicament for the prevention or treatment of an avian viral infection comprising administering to a subject in need thereof a vaccine comprising a therapeutically effective amount of the virus strain DEV SD 70.

According to an embodiment of the application, the subject comprises a chicken, duck, goose, pigeon, quail or ostrich. In an exemplary embodiment, the subject is a duck.

According to an embodiment of the application, 1 × 10 for each subject²TCID₅₀To 1X 10⁸TCID₅₀The vaccine was administered in doses of the strain DEV SD 70/time. In an exemplary embodiment, 5 × 10 for each subject⁴TCID₅₀To 2X 10⁶TCID₅₀The vaccine was administered in doses of the strain DEV SD 70/time.

According to embodiments of the present application, the vaccine is administered to the subject via subcutaneous, intradermal, intramuscular, intravenous injection, oral or inhalation routes. In exemplary embodiments, the vaccine is administered to the subject subcutaneously.

According to embodiments of the application, the vaccine is administered to the subject at least 1 time. In an exemplary embodiment, the vaccine is administered to the subject at a frequency of 2 administrations at 60 day intervals.

In yet another aspect of the present application a recombinant virus is proposed, which is an engineered virus based on the virus strain described in the above aspect, preferably the virus strain DEV SD70, wherein the recombinant virus comprises a foreign gene encoding a protective antigen fragment of waterfowl virus, located in a region of the recombinant virus corresponding to the non-coding region between UL27 to UL26 in the genome of the virus strain.

According to an embodiment of the application, the protective antigen fragment is a gosling plague virus VP2(viral structural protein2) protein, a duck circovirus cap (capsid protein) protein, a goose astrovirus cap (capsid protein) protein, an avian influenza virus ha (hemagglutinin) protein, an avian influenza virus na (neuroamididase) protein, a tembusu virus E protein, and a tembusu virus prM protein. In exemplary embodiments, the avian influenza virus is H5N1, H5N6, or H5N 8. In exemplary embodiments, the protective antigen fragment is gosling plague virus VP2 protein.

According to an exemplary embodiment of the present application, the protective antigen fragment is gosling plague virus VP2 protein having the sequence defined in SEQ ID No. 3.

According to an embodiment of the application, the recombinant virus is for use in the prevention or treatment of at least one of chicken pest disease, duck pest disease and goose pest disease.

According to embodiments of the present application, the recombinant virus comprises a nucleic acid encoding a CMV promoter, a foreign gene encoding a protective antigen fragment of a waterfowl virus, and a nucleic acid encoding a bGH poly adenylation signal.

In yet another aspect of the present application, a pharmaceutical composition is provided, comprising the recombinant virus of the above aspect and a pharmaceutically acceptable carrier.

In the present application, the virus strain DEV SD70 with significantly reduced virus virulence was obtained by serial passage of the isolated virus strain DEV SD04 with strong virus virulence through chick embryo fibroblasts for 70 passages. The novel vaccine prepared by the virus strain DEV SD70 and used for preventing or treating avian viral infection diseases, particularly duck viral enteritis has the characteristics of high virus titer, good immunogenicity, high safety, stability and effectiveness, and can effectively resist duck enteritis virus infection. Furthermore, the inventors have further constructed recombinant viruses based on the strain DEV SD70, which can be advantageously used for the preparation of concatameric vaccines, allowing the insertion of foreign genes directed against protective antigen fragments of different waterfowl viruses in the region corresponding to the non-coding region between UL27 to UL26 in the genome of the strain DEV SD 70. The prepared concatenated vaccine has good immunogenicity, is safe and stable, and can provide effective protection for poultry.

Drawings

FIG. 1 is a phylogenetic tree analysis of the whole genome sequence of the virus strain DEV SD04 according to an embodiment of the present application.

FIG. 2 is a mutation diagram of the strain DEV SD70 relative to the strain DEV SD04 according to an embodiment of the present application.

FIG. 3 is a graph of the one-step growth of the viral strain DEV SD70 according to an embodiment of the present application.

FIG. 4 is a graphical representation of the antibody levels after immunization with the virus strain DEV SD70 according to an embodiment of the present application.

FIG. 5 is a diagram of the construction of recombinant viral rDEV-EGFP according to an embodiment of the present application.

FIG. 6 is a map of the construction of recombinant virus rDEV-VP2 according to an embodiment of the present application.

FIG. 7 is a graph showing the genetic stability of recombinant virus rDEV-VP2 according to the example of the present application.

FIG. 8 is a graph showing the determination of the tissue virus load after immunization with the virus strain DEV SD70 and the recombinant virus rDEV-VP2 according to the example of the present application.

Detailed Description

The present application is made based on the following findings of the inventors: the inventor of the application firstly separates and identifies a novel duck plague virus strain DEV SD04, and the weak virus strain DEV SD70 obtained by continuously passaging 70 generations of the virus strain DEV SD04 through chick embryo fibroblasts can be effectively used as a vaccine for preventing or treating avian virus infection diseases, particularly duck virus enteritis. The weak virus strain DEV SD70 can be further used for developing recombinant virus vaccines, the recombinant virus allows a foreign gene aiming at protective antigen fragments of different waterfowl viruses to be inserted into a region corresponding to a non-coding region between UL27 and UL26 in the genome of the virus strain DEV SD70, and the vaccine containing the therapeutically effective amount of the virus strain DEV SD70 or the single-combined vaccine or the multiple-combined vaccine containing the recombinant virus, which is prepared by the method, has good immunogenicity, high safety and strong stability and can provide effective protection for birds.

The novel duck plague virus strain DEV SD04 of the present application was isolated from diseased duck and the whole genome sequence obtained by sequencing was aligned with NCBI database to find that the homology of the virus strain DEV SD04 with Antatid alphaherpesvirus 1strain SD (MN518864.1) was the highest, reaching 99.93%. The phylogenetic tree results show that the virus strain is a single branch and has the highest affinity with duck herpesvirus 1strain SD (MN 518864.1). Meanwhile, PCR amplification identification is carried out on the virus strain on a UL1 sequence highly conserved in the duck plague virus, and a UL1 sequence sequencing result proves that the virus strain belongs to the duck plague virus. Therefore, the inventor of the present application named this newly isolated duck plague virus strain DEV SD04 and sent it to the common microorganism center of the china microbiological culture collection administration (CGMCC) of the institute of microbiology, china academy of sciences, No. 3, west road 1, north cheng, north, beijing, to be preserved at 12 months and 10 days 2021, with the preservation number of CGMCC 45055.

The inventors of the present application have found that said virus strain DEV SD04 belongs taxonomically to the family herpesviridae, the subfamily α -herpesviridae, the genus marek's virus and can be developed as a vaccine for the prevention or treatment of avian viral infections, in particular duck viral enteritis. Further, the inventor of the present application carries out continuous passage of the separated duck enteritis virus strain DEV SD04 with strong virus virulence through chicken embryo fibroblasts for 70 generations to obtain a weak virus strain DEV SD70 with significantly reduced virus virulence, and sends the weak virus strain DEV SD70 to the China general microbiological culture Collection center (CGMCC) of the institute of microbiology, No. 3 of China academy of sciences, North road 1 institute of Tokyo-Naja, N.Y., in the Beijing city for preservation within 12 months and 10 days in 2021, wherein the preservation number is CGMCC 45056. The novel vaccine prepared by the virus strain DEV SD70 and used for preventing or treating avian viral infection diseases, particularly duck viral enteritis has the characteristics of high virus titer, good immunogenicity, high safety, stability and effectiveness, and can effectively resist duck enteritis virus infection.

The present inventors have also unexpectedly found that the viral strain DEV SD70 has a large genome, many nonessential genes, can accommodate a large capacity of foreign genes, has a large number of insertion sites, is genetically stable, is less affected by maternal antibodies, has a long survival time in vivo, and the like, and thus has potential for the development of recombinant viral vaccines. Further, the present inventors constructed a recombinant virus as a vaccine based on the virus strain DEV SD70, which allows insertion of foreign genes for protective antigen fragments of different waterfowl viruses in a region corresponding to the non-coding region between UL27 to UL26 in the genome of the virus strain, such as position 69207-69658. The obtained recombinant virus can be used for preparing vaccines, in particular to combined vaccines, for effectively preventing or treating at least one of the chicken plague, the duck plague and the goose plague, namely, can be used for effectively preventing or treating the chicken plague, can be used for effectively preventing or treating the duck plague, can be used for effectively preventing or treating the goose plague, and can also be used for effectively preventing or treating the chicken plague, the duck plague and the goose plague. The single combined vaccine or the multiple combined vaccine prepared by the method has the characteristics of good immunogenicity, high safety and strong stability, and can provide effective protection.

Thus, according to one aspect of the present application, there is provided a duck herpesvirus type 1 (anated herpesvirus 1) virus strain belonging to the family herpesviridae, subfamily alphaherpesviridae and genus marek's virus, wherein the virus strain has the genomic sequence shown in SEQ ID No. 1 or a sequence which is at least 90% identical to the sequence shown in SEQ ID No. 1. The "sequence having at least 90% identity" refers to a sequence having an identity represented by any number (inclusive of endpoints) between 90% and 100% with the genomic sequence represented by SEQ ID NO. 1, and may have, for example, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% sequence identity, as well as sequence identity represented by an infinite number of decimal places between two adjacent integers.

In the examples of the present application, the viral strain DEV SD70 has the genomic sequence shown in SEQ ID NO 1. "genomic sequence" as used herein refers to the entire genomic sequence of a viral strain. According to an exemplary embodiment of the present application, said virus strain DEV SD70 has a deposit number of CGMCC 45056.

In the present application, the virus strain DEV SD70 having the genomic sequence shown in SEQ ID NO. 1 is understood by those skilled in the art as the original virus strain. A virus strain having a genomic sequence which is at least 90% identical to the sequence given under SEQ ID No. 1 can be understood by those skilled in the art as a variant virus strain of the virus strain DEV SD70 having the genomic sequence given under SEQ ID No. 1. It is understood by those skilled in the art that a viral strain such as the strain DEV SD70 may be spontaneously mutated or artificially cultured to form a variant strain, for example by nucleotide deletion, nucleotide addition or nucleotide exchange. The "variant virus strain" has highly identical gene sequences and very similar biological functions to the "virus strain DEV SD 70". Wherein the mutated gene does not substantially affect the conserved sequence of the strain DEV SD70 and thus does not affect the genetic stability of the strain DEV SD 70. More specifically, the "variant virus strain" is also a duck plague virus, exhibiting common characteristics of duck plague viruses.

According to embodiments of the present application, the virus strains contemplated herein include the virus strain DEV SD70 as well as variant virus strains of the virus strain DEV SD 70. The variant virus strain has a genomic sequence which is at least 90% identical, such as 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% identical, to the sequence shown in SEQ ID NO. 1. According to an embodiment of the present application, the variant virus strain has the sequence UL1 shown in SEQ ID NO. 2.

In exemplary embodiments, the variant virus strain may comprise the virus strain DEV SD04 set forth herein. The virus strain DEV SD04 has a preservation number of CGMCC 45055. In the present application, the virus strain DEV SD04 may be considered as a variant of the virus strain DEV SD70 in view of the high degree of genomic sequence identity and similar biological functions.

According to an exemplary embodiment of the present application, the viral strain of the present application may be DEV SD04 with a accession number of CGMCC 45055. According to an exemplary embodiment of the present application, the viral strain of the present application may be DEV SD70 with a accession number of CGMCC 45056.

According to an embodiment of the present application, said viral strain DEV SD70 has the sequence UL1 as shown in SEQ ID NO. 2. According to an embodiment of the present application, said viral strain DEV SD04 has the sequence UL1 as shown in SEQ ID NO. 2. Said viral strain DEV SD04 and said viral strain DEV SD70 have the exact same UL1 sequence.

ctatcgtcggccatggtgtccgggttcacttttgtcatgtcttcggccatatctcgagtctggatcgaatgcataacggatgctggcctgagggtctttgaagcgatgcatattcgcgtttccgtggcaggttctgtcatttgggtctccctcgttgacacagccagtacctacaggcgcagtactaaagctcggcttcccgccccacgctgaatttaaggctttctgaaaagccttgacccccacgtcatcacctattgcaacttcagagtccagcaaaaacccagaaatcgcaaggttagggttgacccataatgcccgctgtggacgctcataccacaaaactacctcaatttggggacagtgactgcggataaatactccagacaacaactccgtgctctttggtggtacagctgcataggttgaagtctccgaggtccctgggcggaggcagtccctagatagtatagacaggacatctggagcatacataactccttcgataaccgggtaactacaggaagtttcttcaaaaaaacccattacgactaggatcacgagcgccaatctatgtaaagtccaacccattgtcccgcaccacaagttcggaacgtttcgttcttcggccaccggacgcactacgtgtataacacgatgccgtattatacctacaagtgttttcaagacggccttccatctggaacccat(SEQ ID NO:2)

The duck enteritis virus strain DEV SD04 of the embodiment of the present application is a novel duck plague virus strain obtained by the first isolation and identification of the present inventors, which is identified as a novel virus strain belonging to the herpesviridae, alphaherpesviridae, marek's virus genus, and can be advantageously used for developing a novel vaccine for preventing or treating avian viral infections, particularly duck viral enteritis, and the vaccine prepared thereby has the advantages of good immunogenicity, high safety and effective use.

2311 deletion of nucleotide 5373 (3063nt),

146179-148673 (2495nt) nucleotide is replaced by 388nt of the nucleotide encoding the frame SORFx,

123708 together with the deletion of nucleotide 123907 (200nt),

126731 and 127138 (408nt) nucleotide deletion,

157484-deletion of nucleotide at position 157891 (408nt),

131060-

153561 153562 insert of nucleotide (104 nt).

Wherein, the replacement of the nucleotide at position 146179-148673 (2495nt) with the coding frame SORFx of 388nt refers to the deletion of the nucleotide at position 2495nt of the genome of the strain DEV SD70 relative to the nucleotide at position 146179-148673 of the genome of the strain DEV SD04 and the insertion of the nucleotide at the coding frame SORFx of 388nt in the deleted region at position 146179-148673. That is, the nucleotides of the 146179-148673 region were replaced with 388nt nucleotides of the coding frame SORFx.

The duck enteritis virus strain DEV SD70 of the embodiment of the application is a attenuated virus strain with significantly reduced virus virulence, which is obtained by carrying out continuous passage of 70 generations on the duck enteritis virus strain DEV SD04 with chicken embryo fibroblasts and obtained by separating the duck enteritis virus strain DEV SD04 with strong virus virulence. The novel vaccine prepared by the virus strain DEV SD70 and used for preventing or treating avian viral infection diseases, particularly duck viral enteritis has the characteristics of high virus titer, good immunogenicity, high safety, stability and effectiveness, and can effectively resist duck enteritis virus infection. Furthermore, the present inventors have unexpectedly found that the virus strain DEV SD70 has a large genome, many nonessential genes, can accommodate a large capacity of foreign genes, has many insertion sites, is genetically stable, is less affected by maternal antibodies, has a long survival time in vivo, and the like, and thus can be further used for the development of recombinant virus vaccines.

According to a further aspect of the present application, a vaccine for the prevention or treatment of an avian viral infection is presented, comprising a therapeutically effective amount of the virus strain DEV SD70 having accession number CGMCC 45056. The vaccine has the characteristics of good immunogenicity, high safety and strong stability, can effectively resist avian viral infection diseases, particularly duck viral enteritis, and provides an effective protection effect for poultry.

According to an embodiment of the present application, said virus strain DEV SD70 is a live, attenuated live or inactivated virus strain. In exemplary embodiments, the virus strain is a live virus strain.

The virus strain DEV SD70 used in the vaccine of the present application can be live, dead or inactivated by chemical or thermal means, as described previously. The virus strain DEV SD70 is a weak virus strain with remarkably reduced virus virulence, which is obtained by continuously passaging the separated virus strain DEV SD04 with strong virus virulence through chicken embryo fibroblasts for 70 generations, so that a live virus strain can be used as an effective component of a vaccine. Vaccines with live virus strains are able to induce innate immunity and adaptive immune responses, and thus are advantageous for inducing protective immunity, compared to vaccines with inactivated virus strains. Furthermore, vaccines with live virus strains are able to form memory immune cells that are stimulated by persistent antigens, thus facilitating immunity. The inventor of the application has proved through experiments that the live virus strain has higher safety, immunogenicity and stability as a vaccine.

According to an embodiment of the present application, the viral strain DEV SD70 in the vaccine is at 1 × 10²TCID₅₀/ml or TCID₅₀G to 1X 10⁸TCID₅₀/ml or TCID₅₀In the dosage of/g. In an exemplary embodiment, said viral strain DEV SD70 in said vaccine is at 5 x 10⁴TCID₅₀/ml or TCID₅₀G to 2X 10⁶TCID₅₀/ml or TCID₅₀In the dosage of/g. By using the administration dosage as described above, the vaccine of the present application can safely and effectively prevent or treat avian viral infection, in particular can safely and effectively prevent or treat duck viral enteritis.

According to the embodiment of the application, the vaccine also comprises an adjuvant, so that the high-efficiency specific immune response can be induced, the body protection capability can be improved, the using amount of immune substances can be reduced, and the production cost of the vaccine can be reduced. According to embodiments of the present application, the adjuvant may be one or more of aluminum hydroxide, aluminum phosphate, aluminum potassium sulfate, cross-linked polyacrylic acid polymer, dioctadecyl dimethyl ammonium bromide, an immunomodulatory substance, lactoferrin, or a gamma-interferon inducer.

The vaccine of the present application may be formulated as a systemic or topical agent, and may contain a pharmaceutically acceptable carrier, such as a saline solution for phosphate buffer, distilled water, a water/milk emulsion, a wetting agent, an emulsifying agent, a pH adjusting agent, and the like.

According to a further aspect of the present application, the present application proposes the use of the virus strain DEV SD70 with accession number CGMCC45056 for the manufacture of a medicament for the prevention or treatment of an avian viral infection, comprising administering to a subject in need thereof a vaccine comprising a therapeutically effective amount of the virus strain DEV SD 70.

According to yet another aspect of the present application, there is provided a method for preventing or treating an avian viral infection comprising administering to a subject in need thereof a vaccine comprising the therapeutically effective amount of the virus strain DEV SD 70. The virus strain DEV SD70 has a preservation number of CGMCC 45056.

According to an embodiment of the application, the subject is an avians of the order anseriformes. In exemplary embodiments, the subject is poultry. In exemplary embodiments, the subject comprises a chicken, duck, goose, pigeon, quail or ostrich. In an exemplary embodiment, the subject is a duck. The duck comprises various varieties of Chinese breeding industry.

According to an embodiment of the present application, 1 × 10 is administered to each subject, in particular duck²TCID₅₀To 1X 10⁸TCID₅₀The vaccine was administered in doses of the strain DEV SD 70/time. In an exemplary embodiment, 5 × 10 for each subject⁴TCID₅₀To 2X 10⁶TCID₅₀The vaccine was administered in doses of the strain DEV SD 70/time. By using the administration dose as described above, the vaccine of the present application can safely and effectively prevent or treat duck viral enteritis.

According to an embodiment of the application, the vaccine is administered to the subject via subcutaneous, intradermal, intramuscular, intravenous, oral or inhaled route. In an exemplary embodiment, the vaccine is administered to the subject, particularly a duck, subcutaneously.

According to an embodiment of the application, the vaccine is administered to the subject, in particular a duck, at least 1 time. In an exemplary embodiment, the vaccine is administered to the subject at a frequency of 2 administrations at 60 day intervals.

The prophylactic use and the prophylactic method as described above in the present application have the characteristics and effects of the vaccine as described above and will not be described herein again.

According to yet another aspect of the present application, the present application proposes a recombinant virus which is an engineered virus based on the viral strain described in the above aspect, preferably the viral strain DEV SD70, wherein the recombinant virus comprises an exogenous gene encoding a protective antigen fragment of waterfowl virus, the exogenous gene being located in a region of the recombinant virus corresponding to the non-coding region between UL27 to UL26 in the genome of the viral strain, such as position 69207-69658.

The recombinant virus of the embodiments of the present application is a virus obtained by genetically engineering the strain DEV SD70 by molecular biological means, which allows the insertion of foreign genes for protective antigen fragments against different waterfowl viruses in the region corresponding to the non-coding region between UL27 to UL26 in the genome of the strain, such as position 69207-69658, and can be advantageously used for the preparation of a single-or multiple-combination vaccine. The single-combined vaccine or the multiple-combined vaccine prepared by the method has good immunogenicity, high safety and strong stability, and can provide effective protection for poultry.

According to an embodiment of the application, the protective antigen fragment is a gosling plague virus VP2(viral structural protein2) protein, a duck circovirus cap (capsid protein) protein, a goose astrovirus cap (capsid protein) protein, an avian influenza virus ha (hemagglutinin) protein, an avian influenza virus na (neuroamididase) protein, a tembusu virus E protein, and a tembusu virus prM protein. Thus, by using the recombinant viruses specifically designed in the present application, a single-or multiple-combination vaccine can be prepared.

In exemplary embodiments, the protective antigen fragment is gosling plague virus VP2 protein. Thus, the DEV SD70 virus comprising the gosling plague virus VP2 gene can be constructed as a multiple vaccine for the prevention or treatment of both duck plague and goose plague.

According to an embodiment of the application, the CSFV VP2 protein has the sequence defined in SEQ ID No. 3.

TAPAKKNTGKLTDHYPVVKKPKLTEEVSAGGGSSAVQDGGATAEGTEPVAASEMAEGGGGALGDASGGADGVGNASGNWHCDSQWMGNTVITKTTRTWVLPSYNNHIYKAITSGTSQDANVQYAGYSTPWGYFDFNRFHCHFSPRDWQRLINNHWGIRPKSLKFKIFNVQVKEVTTQDQTKTIANNLTSTIQVFTDDEHQLPYVLGSATEGTMPPFPSDVYALPQYGYCTMHTNQNGARFNDRSAFYCLEYFPSQMLRTGNNFEFTFDFEEVPFHSMFAHSQDLDRLMNPLVDQYLWNFNEVDSSRNAQFKKAVKGAYGTMGRNWLPGPKFLDQRVRAYTGGTDNYANWNIWSNGNKVNLKDRQYLLQPGPVSATHTEAEASSIPAQNILGLAKDPYRSGSTTAGISDVMVTDEQEVAPTNGVGWKPYGKTVTNEQNTTTAPTSSDLDVLGALPGMVWQNRDIYLQGPIWAKIPKTDGKFHPSPNLGGFGLHNPPPQVFIKNTPVPADPPVEYVHQKWNSYITQYSTGQCTVEMVWELRKENSKRWNPEIQFTSNFSDRTSIMFAPNETGGYIEDRLIGTRYLTQNL(SEQ ID No:3)

According to an embodiment of the application, the recombinant virus is for use in the prevention or treatment of at least one of chicken pest disease, duck pest disease and goose pest disease. The recombinant virus can be used for preventing or treating fowl plague. The recombinant virus can be used for preventing or treating duck plague. The recombinant virus can be used for preventing or treating goose plague. The recombinant virus can also be used for preventing or treating chicken plague, duck plague and goose plague.

In the present application, in contrast to the strain DEV SD70 described above, the recombinant virus based on strain DEV SD70 comprises a foreign gene encoding a protective antigenic fragment of a waterfowl virus in a region corresponding to the non-coding region between UL27 to UL26 in the genome of strain DEV SD70, such as position 69207-69658. Thus, the recombinant virus can be used for preparing a vaccine composition for preventing or treating infectious diseases caused by waterfowl virus. In an exemplary embodiment, the recombinant virus is rDEV-VP2, having the structure shown in FIG. 6.

According to yet another aspect of the present application, there is provided a pharmaceutical composition comprising the recombinant virus of the above aspect and a pharmaceutically acceptable carrier. According to an embodiment of the present application, the pharmaceutically acceptable carrier includes a saline solution for phosphoric acid buffering, distilled water, a water/milk emulsion, a wetting agent, an emulsifier, a pH adjuster, and the like. The pharmaceutical composition has the characteristics and effects of the recombinant virus as described above, and is not described in detail herein.

In the present application, the pharmaceutical composition may be used as a vaccine composition. The vaccine composition can be used for preventing or treating infection caused by waterfowl virus, particularly duck viral enteritis. The vaccine composition may be a single combination vaccine. The vaccine composition may also be a concatameric vaccine.

The various features of the vaccine comprising the strain DEV SD70 described in the above aspects, such as the subject of administration, the dosage administered, the route of administration, the frequency of administration and the efficacy of the treatment, are equally suitable for use in the pharmaceutical compositions of the present application and will not be described in any further detail herein.

According to yet another aspect of the present application, there is provided a method for preventing or treating an avian viral infection comprising administering to a subject in need thereof a pharmaceutical composition comprising a therapeutically effective amount of a recombinant virus.

According to yet another aspect of the present application, the present application proposes the use of a recombinant virus as described above for the preparation of a medicament for the prevention or treatment of an avian viral infection comprising administering to a subject in need thereof a pharmaceutical composition comprising a therapeutically effective amount of the recombinant virus.

The control method and control use against recombinant viruses described here have some of the features of the control method and control use against the vaccine comprising the virus strain DEV SD70 described in the above aspects and will not be described here again.

According to yet another aspect of the present application, there is provided a method of producing the recombinant virus of the above aspect, comprising: in a strain described on the basis of the above, preferably the strain DEV SD70, a foreign gene encoding a protective antigen fragment of a waterfowl virus is inserted, said foreign gene being located in a region of the recombinant virus corresponding to the non-coding region between UL27 and UL26 in the genome of said strain, such as position 69207-69658.

According to an embodiment of the present application, the protective antigen fragment is gosling plague virus VP2 protein, duck circovirus Cap protein, goose astrovirus Cap protein, avian influenza virus HA protein, avian influenza virus NA protein, Tembusu virus E protein, Tembusu virus prM protein, and the like. In an exemplary embodiment, the protective antigen fragment is gosling plague virus VP2 protein.

To assist in the understanding of the present application, the following embodiments are shown. However, the following examples are provided only for easier understanding of the present application, and the present application is not limited to the following examples.

Examples

Example 1: isolation and characterization of the Virus Strain DEV SD04

And (3) virus separation: an unknown strain was isolated from diseased duck bodies submitted to farmers in 2004. Firstly, separating and identifying bacteria, and eliminating bacterial disease infection through identification. And (3) selecting liver and spleen organs with obvious hemorrhagic necrosis lesions, and grinding the organs to prepare tissue suspension. Filtering for sterilization, inoculating 8-day-old duck embryo by allantoic membrane inoculation, discarding duck embryo dead within 24 hr, collecting embryo and allantoic fluid after embryo death, and observing embryo severe hemorrhage and liver necrosis. Several serial passages were performed in the same manner to make it fully adapted to duck embryo proliferation. DNA was extracted from the collected allantoic fluid, specific primers were designed for the highly conserved gene sequence UL1 region of duck plague virus, and PCR was performed as shown in Table 1.

TABLE 1 specific primers for DEV Strain identification

Agarose gel electrophoresis showed that a specific band appeared at 1116bp, and the sequencing result also confirmed the UL1 sequence, thereby confirming that the isolated virus strain was a duck plague virus, named DEV SD strain.

Whole genome sequencing: to obtain the sequence of this strain, a strain after passage 4 (i.e., DEV SD04) was selected for genomic sequencing. DNA samples of this strain were sent to the vast Biotechnology Limited of the upper sea for high throughput sequencing, and whole genome sequencing was performed on the strain DEV SD 04. The whole genome sequencing result shows that the whole genome of the virus strain DEV SD04 is 160663 bp. The whole genome sequence of the virus strain is compared with NCBI database, and the homology with duck herpesvirus 1strain SD (accession number: MN518864.1) is the highest and is as high as 99.93%. The phylogenetic tree results showed that the virus strain was a single branch with the highest affinity to duck herpesvirus 1strain SD (accession number: MN518864.1) (see FIG. 1). Therefore, the virus strain is determined to be a newly separated duck plague virus strain.

Pathogenicity of DEV SD 04: taking 10 ducklings of 20 days old at 5 × 10⁴Dose of DEV SD 04/dose of TCID50 was injected subcutaneously via the back of the neck to inoculate each duckling. The disease and death conditions of the inoculated ducklings are observed, after 3 days of inoculation, the ducklings have depression, drooping necking, jelly exudation and head swelling under the skin of part of duck heads, pathological changes such as liver and spleen necrosis hemorrhage, intestinal tract hemorrhage necrosis, pancreatic hemorrhage and the like are examined, and the results are shown in table 2. The ducklings died after 4 days from inoculation. The ducklings died to reach a peak period 5 days after inoculation. The results indicate that the duck plague strain DEV SD04 is very virulent, causing the infected duck to die.

TABLE 2 pathogenicity test of Duck plague Virus DEV SD04

Microbial deposit of the strain DEV SD 04: the virus strain DEV SD04 is used as an original strain and is delivered to China general microbiological culture Collection center (CGMCC) of microbiological research institute of China academy of sciences No. 3 of West Lu No. 1 of the Navy region in Beijing as 12 months and 10 days in 2021 for preservation, and the preservation number is CGMCC 45055.

Example 2: breeding and biological characteristics of virus strain DEV SD70

2.1 Breeding of Weak Virus strains

After adaptation of the virulent duck plague virus strain DEV SD04 for 8 generations on duck embryo fibroblasts, Specific Pathogen Free (SPF) Chicken Embryo Fibroblasts (CEF) were inoculated. CEF cells were prepared according to the conventional method, and the duck enteritis strain DEV SD04 was diluted appropriately and inoculated into a vial full of a monolayer of CEF cells, and CEF cytopathic effect was observed under an inverted microscope at regular intervals. Virus fluid was collected when around 80% of the cells exhibited cytopathic effect (CPE). In the same manner, serial passages were performed in the prepared CEF cells to be attenuated, and serial passages were performed for 70 generations, and the attenuated virus strain, designated duck enteritis virus strain DEV SD70, was obtained by clonal purification. DNA samples of this strain were sent to the vast Biotechnology Limited of the upper sea for high throughput sequencing, and whole genome sequencing was performed on the strain DEV SD 70. The whole genome sequencing result shows that the whole genome of the virus strain DEV SD70 is 154685bp, which is shown as SEQ ID NO. 1.

Microbial deposit of the strain DEV SD 70: the virus strain DEV SD70 has been delivered to China general microbiological culture Collection center (CGMCC) of microbiological research institute of China academy of sciences No. 3, West Lu No. 1, North Cheng, Ministry of Japan, Beijing, 12 months and 10 days in 2021 for preservation, with the preservation number of CGMCC 45056.

2.2 biological Properties

2.2.1 reproductive Properties

The duck plague strain DEV SD70 was inoculated with SPF-grade CEF cells at a multiplicity of infection (MOI) of 0.01 and the virus growth curve was measured in one step and the results are shown in FIG. 3. The virus content reached the highest value at 72 hours in culture, 105.6TCID50/0.1ml, indicating that the strain DEV SD70 has good propagation properties in CEF cells.

2.2.2 safety and stability

The age of 7 days ducklings and the age of 30 days ducks are 10 respectively, and the number is 2 multiplied by 10⁶TCID₅₀The dose of the virus strain DEV SD 70/time was used to immunize each duck subcutaneously on the back and neck. After continuously observing for 14 days, each duck has no pathogenic symptoms and is healthy, and the results are shown in table 3, which indicates that the duck plague virus strain DEV SD70 is very weak in toxicity and generates good safety for the ducks.

TABLE 3 pathogenicity of Duck plague Virus DEV SD70 to ducks of different ages in days

Note "-" indicates no clinical symptoms

Taking 25 SPF chick embryos of 8 days old, dividing the chick embryos into 5 groups, each group containing 5 SPF chick embryos, and injecting 10 SPF chick embryos into the groups^-2To 10^-6Dilution of duck plague strain DEV SD 70. After 14 days of continuous observation, the results are shown in Table 4, and DEV SD70 at each dilution does not kill chick embryos, indicating that the duck plague strain DEV SD70 is very virulent, does not kill chick embryos, and is not pathogenic to chick embryos.

TABLE 4DEV SD70 pathogenicity of chick embryos

At 2X 10⁶The dose of the viral strain DEV SD 70/time/single of TCID50 was inoculated subcutaneously to the back of the neck with 10 ducklings of 5 days old. The tissues were dissected and killed 3 days after immunization and organs were harvested to prepare 20% tissue suspensions. The next generation was inoculated in the same manner and returned continuously for 5 generations. The toxic amount of tissues of each generation is measured by using a SYBR Green fluorescent dye method, and the result is shown in a table 5, which indicates that the duck plague virus strain DEV SD70 can be detected only in part of ducks in the P1 generation, the duck plague virus strain DEV SD70 is a limited replication in the body of ducks, and has good safety for ducklings and biological characteristics with good stability.

TABLE 5 detection of viruses in tissues of Return Strength experiment

2.2.3 immunogenicity

At 5X 10⁴The dose of the viral strain DEV SD 70/time/single of TCID50 was inoculated to 8 sheldrakes subcutaneously on the back of the neck. 60 days after immunization, at 1X 10⁵The second immunization was carried out with the dose of the strain DEV SD 70/time of TCID 50. Blood was collected at fixed points and the antibody levels were determined using a blocking ELISA method. The results are shown in FIG. 4, where the antibody started 7 days after immunizationPositive conversion, the antibody level can reach a higher level 15 days after immunization, and the antibody positive can still be detected 60 days after the first immunization. The second immunization is carried out 60 days after the immunization, the antibody level is obviously increased and maintained for a long time until the detection period is finished, and the antibody level can still be maintained at the blocking rate of more than 50 percent 140 days after the immunization.

At 5X 10⁴The dose of the viral strain DEV SD 70/time// single of TCID50 vaccinated with 10 ducks subcutaneously on the back of the neck. 15 days after immunization, duck plague virulent strain DEV SD04 was administered in a 100-fold minimal lethal dose to each duck. The disease and death conditions of the ducks are observed every day, the DEV SD70 immune group ducks do not have any disease symptoms, and the ducks survive healthily during the observation period; all ducks in the non-immunized group (DMEM group) die, obvious morbidity symptoms appear, and the caesarean section disease is changed into typical duck plague symptoms, such as hemorrhagic necrosis of the liver and the spleen and hemorrhage of the intestinal tract. The results are shown in table 6, which demonstrates that the duck plague virus strain DEV SD70 is able to protect ducks well against the attack of virulent strains of duck plague virus. The results demonstrate that this strain, DEV SD70, has good immunogenicity.

TABLE 6DEV SD70 Duck protection

2.2.4 Whole genome sequence

DNA samples of the duck plague strain DEV SD70 were sent to the vast Biotechnology Limited in the Shanghai and whole genome sequencing of this strain DEV SD70 was performed by high throughput sequencing technology. Sequencing results showed that the whole genome of the virus strain DEV SD70 was 154685 bp. Through sequence alignment analysis, comparing the 154685bp genome of the strain DEV SD70 with the 160663bp genome of the strain DEV SD04, DEV SD70 was found to have the following mutations compared with DEV SD 04: the following fragments were deleted, deletion of 3063nt at nucleotide 2311-5373, deletion of 2495nt at nucleotide 146179-148673 and replacement with 200nt at nucleotide 388nt of the coding frame SORFx, 123708-123907, deletion of 408nt at nucleotide 126731-127138 and nucleotide 408nt at nucleotide 157484-157891, and insertion of 104nt between 131060-131061 and 153561-153562. The above mutations have the greatest effect on the proteins US8(gE protein) and US5(gJ protein), such that the gE and gJ protein structures are altered, wherein in US8 the base 592 is mutated from C to a; in US5, a base G is inserted between the 440 (G) and 441 (G) positions. The genomic sequence changes of DEV SD04 and DEV SD70 before and after passaging are shown in FIG. 2.

Example 3: construction of a recombinant Virus based on the Virus Strain DEV SD70

Recombinant viruses were constructed based on duck plague strain DEV SD 70. Specifically, foreign genes encoding protective antigen fragments of different waterfowl viruses were inserted into the region corresponding to the non-coding region between UL27 to UL26 in the genome of the virus strain DEV SD70 to construct corresponding recombinant viruses. The recombinant virus is used as a single vaccine or a multiple vaccine to prevent or treat duck plague and diseases caused by corresponding waterfowl viruses.

3.1 construction of rDEV-EGFP recombinant viruses

The selected weak virus strain DEV SD70 was used as a viral vector. The EGFP expression cassette, which was easy to screen for identification, was first selected and inserted into the non-coding region between ul27 and ul26 of the genome of DEV SD70 in order to obtain recombinant viral rDEV-EGFP which can express green fluorescent protein. Construction strategy referring to FIG. 5, the rescue procedure for recombinant viruses is specifically described below.

Viral rescue was performed using CRISPR/Cas9 system to construct gRNA plasmids expressing Cas9 protein. Transfection procedures for rescuing virus were: the chicken embryo fibroblasts were seeded in 12-well plates (about 4X 10) using a conventional method⁵One cell/well), and transfection is carried out when the cells grow to 80% -90% adherent; using the Opti-MEM culture medium to wash the cells for 3 times to remove the serum in the original culture medium, adding 400 μ l of the Opti-MEM culture medium to each well, and placing at 37 ℃ for standby; then, taking a sterile EP tube to dilute DNA, diluting gRNA plasmid and donor DNA fragment into Opti-MEM culture medium by 1.5 mu g respectively, mixing uniformly, and adding 3 mu l of P3000 reagent; another EP tube was used to dilute the liposomes, and 3.4. mu.l of lipofectamine 3000 was diluted in Opti-MEM medium; two EP tubes for liquid lighteningAnd (3) mixing the mixture evenly, reacting the mixture for 15min at room temperature, then adding the mixture evenly into a treated cell culture plate, replacing the maintenance solution 4 hours after transfection, and inoculating the weak virus strain DEV SD70 with the toxicity of 1.0 MOI 16 hours after transfection. The state of chicken embryo fibroblasts is observed every day, virus harvesting is carried out for about 48-60 hours, the harvested virus liquid is inoculated into two 6-well plates according to the proportion of 1:2 so as to remove the transient expression of plasmids, and the recombinant viruses expressing green fluorescence can be observed under a fluorescence microscope after 48 hours. If the recombinant virus expressing green fluorescence is seen, the purification is carried out by a method of sequentially 5 rounds of plaque purification and limiting dilution alternation until a pure recombinant virus rDEV-EGFP is screened out. Amplification and sequencing by PCR was performed to identify the correctness of the EGFP expression cassette sequence and the accuracy of the insertion site.

3.2 construction of recombinant Virus rDEV-VP2

The recombinant virus rDEV-VP2 was constructed in the same manner as described above. In order to facilitate screening of recombinant viruses and increase of homologous recombination and rescue efficiency of the recombinant viruses, the embodiment of the application knocks out the ORF of the EGFP by using a double-gRNA knocked-out CRISPR/Cas9 system, further inserts a genome encoding a parvovirus structural protein VP2 into a region corresponding to an EGFP expression frame of the recombinant virus rDEV-EGFP by using a repair mechanism (namely, a homologous mediated repair mechanism (HDR)) of a cell, and obtains the duck enteritis recombinant virus expressing the parvovirus structural protein VP2 through screening and purification, wherein the duck enteritis recombinant virus is named as rDEV-VP 2. Construction strategy referring to FIG. 6, the rescue procedure for recombinant virus rDev-VP2 was similar to that described above for rDev-EGFP recombinant virus.

Example 4: biological characteristics of recombinant virus rDEV-VP2

4.1 safety of recombinant Virus rDEV-VP2

The number of 7-day-old ducklings and 30-day-old ducks is 1.2X 10⁶Each duck was immunized subcutaneously on the back and neck at doses of rDEV-VP 2/dose of TCID 50. After continuously observing for 14 days, the ducks have no disease symptoms and are healthy and alive, and the results are shown in a table 7. The result shows that the duck plague recombinant virus rDEV-VP2 has weak toxicity and good safety to ducks.

TABLE 7 pathogenicity of recombinant virus rDEV-VP2 on ducks of different ages in days

Note: "-" indicates that no clinical symptoms occurred

At 1.2X 10⁶The dose of rDEV-VP 2/time/single of TCID50 is used for subcutaneous immunization of 10 ducklings of 5 days old on the back and the neck. The immunization was performed 3 days after the killing, and 20% tissue suspension was prepared from organs. The next generation was inoculated in the same manner and returned continuously for 5 generations, and the toxic tissue mass was measured for each generation using the SYBR Green fluorescent dye method, the results of which are shown in Table 8. The result shows that the duck plague recombinant virus rDEV-VP2 can be detected only in part of ducks in the P1 generation, and the duck plague recombinant virus rDEV-VP2 is copy limited in duck bodies, has good safety to ducklings and has stable biological characteristics.

TABLE 8 detection results of viruses in tissues of rDEV-VP2 Return experiment

4.2 stability of recombinant Virus rDEV-VP2

The recombinant virus rDEV-VP2 was serially passaged for 20 generations on CEF cells, IFA and Western Blot were performed every 5 generations to identify the expression of VP2 protein, and sequencing was performed to verify the presence or absence of base mutation.

Multi-step growth curve determination: the purified recombinant virus rDEV-VP2 and the weak virus DEV SD70 were inoculated into CEFs (modified strains of bacteria) with an MOI of 0.01, which were prepared in advance, and were grown to about 90%, and were harvested at 12-hour intervals, and the virus titer TCID50 at each time point was calculated from the harvested virus fluid by the Reed-Muench method, and a multi-step growth curve was plotted by GraphPad Prism 7.0, and significant difference analysis was performed. The results are shown in FIG. 7, the recombinant virus can be stably expressed in CEF cells, no mutation occurs, and no change is caused in growth characteristics, which indicates that the recombinant virus rDEV-VP2 has good stability. Meanwhile, it is shown that the weak virus strain DEV SD70 can be used as a recombinant virus, and the insertion site corresponding to the region of 69207-69658 bit of DEV SD70 genome is suitable for the insertion of a foreign gene.

4.3 immunogenicity and protective efficacy of recombinant Virus rDEV-VP2

68 Beijing ducklings of 6 days old are randomly grouped, and the 6 days old ducklings are immunized by rDEV-VP2 and DEV SD70 through neck and back subcutaneous parts. 14 days after immunization, 1.5X 10⁵ ELD 50/dose alone was challenged with parvovirus virulent DEV SD 04. Ducklings are dissected and killed 4 days, 8 days and 12 days after the toxin is attacked, the tonsils of the liver, the spleen, the thymus, the bursa of fabricius and the caecum are collected, and the virus-carrying viscera is detected by PCR amplification. 30 days after immunization, at 1.5X 10⁵Dose of ELD 50/alone a second challenge with parvovirus virulent DEV SD04 was performed. The experimental duck is killed by dissecting 4 days after the challenge, and the viscera is collected and the PCR amplification detection of the viscera toxicity carrying amount is carried out.

The experimental embodiment is shown in table 9. The results are shown in fig. 8, compared with the method for immunizing DEV SD70 without foreign gene (i.e., blank control group), the virus content of duck visceral organs after the recombinant virus rDEV-VP2 is immunized is significantly lower, which indicates that the recombinant virus rDEV-VP2 can more effectively inhibit the replication of parvovirus and provide effective protection.

TABLE 9 rDEV-VP2 immune protection experiment design scheme

Note "-" indicates that no treatment was made

In conclusion, the recombinant virus rDEV-VP2 has good safety, immunogenicity and stability, can generate a specific antibody aiming at the insertion protein, and provides good protection for the immunized duck.

Example 5: construction and biological characterization of other recombinant viruses

5.1 Duck plague strain DEV SD70 can be used as a vector of recombinant virus, and the expression cassette of Cap protein of goose astrovirus is inserted into the region corresponding to the 69207-69658 site of the genome of the strain DEV SD70 according to the conventional biological technology to construct recombinant virus as a bivalent vaccine. Experiments prove that the constructed recombinant virus as a bivalent vaccine can effectively prevent duck plague and goose kidney induced gout.

5.2 Duck plague strain DEV SD70 can be used as a vector of recombinant virus, and the expression cassette of Cap protein of duck circovirus is inserted into the region corresponding to the 69207-69658 site of the genome of the strain DEV SD70 according to the conventional biological technology to construct recombinant virus as vaccine. Experiments prove that the constructed recombinant virus as a vaccine can effectively prevent duck plague virus and duck circovirus.

5.3 Duck plague strain DEV SD70 can be used as a vector of recombinant virus, and the expression cassette of avian influenza virus HA protein is inserted into the region corresponding to the genome 69207-69658 of the strain DEV SD70 according to the conventional biological technology to construct recombinant virus as vaccine. Experiments prove that the constructed recombinant virus as a vaccine can effectively prevent the chicken plague and the duck plague.

5.4 Duck plague strain DEV SD70 can be used as a vector of recombinant virus, and the expression cassette of the E protein of Tembusu virus is inserted into the region corresponding to the 69207-69658 site of the genome of the strain DEV SD70 according to the conventional biological technology to construct recombinant virus as vaccine. Experiments prove that the constructed recombinant virus as a vaccine can effectively prevent duck plague and goose plague.

In this application, the terms "one embodiment," "some embodiments," "an example," "a specific example," or "some examples" and the like mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the application. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

Various embodiments or examples and features of various embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.

Sequence listing

<110> university of agriculture in China

<120> vaccine for preventing or treating avian viral infection

<130> E1218067

<160> 5

<170> PatentIn version 3.5

<210> 1

<211> 154685

<212> DNA

<213> Artificial sequence

<220>

<223> DEV SD70 Whole genome sequence

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caaacgttat aaacggcacg aaaagacgcc caaatgtcta ttttatggtc gtataactga 5520

gttgaagttt ttgacaaaca aaggccaggg taccatgaat atagagacta ttgatgcgtg 5580

taactcttcg gatgagtgat aaagaaatta ttataccttc gtgttatctt tctgctttct 5640

aaaccactaa acgactatgt aaatcttcaa tttgtttatc tccgttcgtg agctattaac 5700

atggttgtaa aaaagacata tataaagaca caaaacaact ttaccgcgtc tgtgttgtta 5760

ttatagacgg ttgctcagat agaaagaaag aattatctct ttaattagta aagttaagca 5820

tcgaagatat ttaatattct attgccatca aaatgacgga aactagagtc atagctattt 5880

aaggactgct ctttttattt cttaaaacat ttcattacaa taaaagtact tcccgtgcaa 5940

aaatataggc gagattacat aactcgaata caatttgcac tgttggtcgt ggcaatcgtg 6000

caaatgttcg tctatcatct ccaagatacc agtgatgcac gctccgggta tatagcttct 6060

taataatcct aaaggatcga cgtcaccaag aggaatatag tccgatggtc gtttaagagc 6120

cctggacaac ttaacgaggg taatgaaaag aaagctcggt aggtcgacta tatcgccctt 6180

gtctcctctc ttcagcattt catcaaatga tctgcgcgtt ttcatctcgc ggcataagaa 6240

aatggggcgc aattttaact tcagattttc cagcaaagag ttaacagtcg cgacaatcgg 6300

atcgttagga atcaatggta ggccataaaa ctcatgcatc tttaaccatg ccataatctc 6360

atcgcatgac gctaatgcag ctattaagtc ttcattgcgc agaactgcag cccgcaggcc 6420

aatagccgca ctagatgcca attgtctaat ctcaaacatc cgcctcaact ctttcccgat 6480

taaacatagc gtatgccatg aaactctacg accttcgggt cctgaattcg cattatcaaa 6540

agatagtaca gatgcccatg ggtgttcggc ggttgttcgg aagcctccat tctgcactcc 6600

tcctgaaagt gtggcacggc atctttcaaa tatactattt atgcgttgct gaattgtagg 6660

tcggttaatg gctcgttggt cccggccttc gtgtgctggg cttgagcgtt gacttctacg 6720

gctttgttta aaatcacttt tatctgacat tcggcgcctg tctcggtttg gaggcgagcg 6780

gtcagcgtcg ccaactgttg cttcattatc agattgcatt ttagattgat ggcgctggtc 6840

gtgatcagaa ctacgtcgtt tattgccgtg atggccataa ggcgatccgt ggtttcgggg 6900

gctgtgtcgc ttagccctcg ctcgtttaac atatttccca gtatcatgat cggagggttt 6960

tttcttttca ttagcagtag ttccgcgatc ctcgtctgtc gcaacatttg gattctcaga 7020

cttgccgggc tccatagagt ctagtataat ccagtcgttg ctttgcgtct cggcagtgcc 7080

gtcctcgtat ttgttctgtt tgatatctgg ttttaaaccg tcgttattaa catttcctgt 7140

cttaggtcga gcaaacattt cctcgtccgt gtcgccagaa aaactattgg tctctgctcc 7200

ggagtcgtaa tccaataggc tcatggcatc atcttcatca tacttgtagc ctggggcggg 7260

tttagcactg caggccatga cgttgggcag gttcgttacc tctagtattg tttatttcta 7320

gaactttgtc aatctaacat ttggccaaac tatatataca catcaatatg ccggcccact 7380

tttccccaaa caccacacgt attacaagta ggcgtggaac aacacattta ttcaaattaa 7440

aatgcaaatt tatgcactga acaacatttc ctgtactttt ctctcatacc ataaaaaaag 7500

gaataccagc cctattatcg atgccaaatg caatagttta acaaacagcc cagacattag 7560

agttgcacaa caattagcac agactgacgt tagtccagac ataacacggg attgtttacg 7620

ttctgtcgtc tcccctacca ttatcttttt atctgtaggc ccgggggtca acattacaaa 7680

tagctctaca atagctacta tagctacata aatgcctgtt ataaccttaa aatacagtgg 7740

gtatgccgcg gcgcatggtc catgcagcgc ggaaaatgcc atcataacac aacattgagc 7800

tataccatgt gtcgttactt ctaataaaaa taaaactgca gttgtcttat gtcgtactga 7860

cgatgttatt gggtcattta caaatccacg gctgaaagat gatctgattg tagccaattc 7920

gcacattaag cgtgccatct tagaatattt acacccggca acgttgctcg ctattacacg 7980

tacggcataa ctaagaggat agcgagtggg cgaaatgaac tccttttgta ctctaaagac 8040

tccgaacatg cgacgatgct gtcgcataca tataaatgtg gcataaaaca taacagttaa 8100

ggaagcaaaa aaaaaatgcg ttgtccagat agaagcgacg caatttctag ctgcagatac 8160

aatacgtatt ctattgcgaa tggcttggtt gcgatttaac gaaactgctg ccgttttatc 8220

caagacgtca acaccacaaa tagcgtcaaa tttgttggtc atgctggccc cattaggtga 8280

agaaataaaa agttgtgtct cgttatacgc ttcccatgtc acattgagag cgccggaaga 8340

tgcgttttct ataggcgaga cggcgtagac gcaccaatgt cttcctacca ttccattagt 8400

tccaaaccat agcaagaaaa cagtataggt agtcgcgagt aacagtagcg ccatgaagac 8460

gatgcgtccg cctaagaaca ttattggctg gttttgctgt taattgctcc tcctatgtct 8520

acagtaaaca gggtatttag tttattgctt ccacacttgg tcgcgaagca ctgctggcta 8580

agtgatgcgc agaggcggcc atgcgcgtcg acgcttatcg caaggaatgt tctcgcagat 8640

cctttcgtcg tacgtgtatg tttatagcgc acgcaagaaa acccattgag atgtgagctg 8700

cgtgaaggaa tgagttgtag taaaagccaa tctgccttca aaattctaca gcgcgcgatc 8760

gcattcttat actcatgggc gtacccgacg aagtgatttt ccaggtagcg tatcaactct 8820

tctagaatga aacacgtggc aaattcctcc actgctgtac tatctgttgc acataccccc 8880

aacacgccca atgcctcaac gagagatgta cgcggggcga aaaacctatc gcggttatga 8940

ttagtctttg catcgaaaaa actgatatag tctccaccga ggctgtatat aacatgatga 9000

ggcatttcat tcacgatagg aagagaatga aaatggaaat tgcgtggttc gaaatggcca 9060

gatataaatg agttcacatc attacatgat tctggaacta cgatgaatgg cagtaatctt 9120

ccgacaatgt tattttctgt tgtcaccttt cccaaaaatg gtaaccgcaa acttctacca 9180

ttgccatata tacccgtgtc aatgaagtta tagtctttca cgtagatact tatggcttct 9240

gcgaatgagc gatcaagcag caccgcttgc tgtataagac gcgctatacc taccattgtt 9300

tttgaaccaa ataataaata cggcgttggc actggaatgg aaatgcgcat acctatcttt 9360

ttaacgcaat cacatgtaac gtttgatgcc accgtatcta aacggttcgt tagttcggat 9420

ttaatgtcaa tttcttcctc ttgttcacta cactcttcat ttggcgcgct atcgagcatt 9480

tcgaataacg ctgtttcgtc attatagccc tcaaaaccgc tgtcataact atcaaacggt 9540

tcatctaaga tatgcgaggc gtcatgatat agactagggt cttctaacat ctcataatgc 9600

tcgtctgtct ctaatccact gctatgctga ttgtctacat ttttacttgt ttttacacgc 9660

ccatcgttag tacatgcgct cttataaaaa taacatgggt aagaagacca atctacatca 9720

gttttaggca ttataagcga tagtgcggtg agcgccccct ttctaaaccc acgcatagct 9780

gtatgcagtg caatcaatgg tactgcttgc tttagatgca tatccacatc taggattata 9840

tttcctatcg ccaaggagcc attaaatatt tcattcctat ttgcatacat ctgctcgcct 9900

actattgatg atgcgatgaa tatactacgt tggttttcaa aactgctagt tacgatagac 9960

gttattttca tatcgactgc agctaagtca gatagcgata gttgttctct catgcccagt 10020

gatgtttgta ttaccggttc aaatgtatcg ttttttccag ttatgcgact ccatacatcg 10080

ccggttataa ttccaaatgc ctgtttgcca ttgggcatac caatcctata agatggtagt 10140

ggtatattgc tcgtcttgga accgataaat agacatgctg gcgccatagt tccggcgggt 10200

ggctctgttg ctgcgatagt tagtaaccgt tttacgatgc catagccgga gccctgtata 10260

ccaatctgaa attgtgatgg catccaatca ccttgttcat actgtaactc gtcgcaattg 10320

tcacggttgt atgaattgtc agaagggtcg ttttgacgat cccctgtctg gtgtaatgat 10380

ggggctgacg gattggactc tggccaacct cggcgaacta aagctgcctc caccttatct 10440

aatcttcgtg caatttgcgt tgcataatcc catataccga caacggtgtt gccatgggtt 10500

tgtttaaatg tatctttgtg ataagtcttt gagtttacat acatttttgc cagatccggc 10560

gttaaaacta atacctttgg caccacattt tgtttaatgt aatcatctat atttaactgg 10620

gctctaacat gtgagaagaa actttctaca gctcgttccc ccatgccaga ttttcctata 10680

atattcattt cagtttcctc aggttctcga actgtcacag cattaagatg attcgtcagt 10740

tgttcgcgat taaaacattc aaaatgtgct aaatatacat atgtaacaaa atccttatca 10800

gatatacgta aacagccccg atcattcacc aagaattcgc gcaaggctcc aacctcggcc 10860

atatctgcag caatgcgtcg ctctatatat gtggctagca tcgatgcgat gcgccctcta 10920

gcataattac tactacaaca gaatttcgaa aggagtgcaa acgaggtaag gtctgatggt 10980

acaagaccgg ttgggttagt accaacgttt acattgtatg ttccacatag atcccttatt 11040

gcctgaagat cgtaatccga acccctttca ccacattcgt gtgcttgtag taaatagtag 11100

cgcgttccca gtacaagcgt cttttcgtcg ggcccaaatt tagacacgta ccagaacgga 11160

gtaactaccc ccccgaaggc cctgcgatac gccgccagaa cgcgctgctc atgctgaata 11220

tataatgccg caaatccacg ttggccgcca ggataagtgc tcgttaaagc tttcacagag 11280

tttgtagtca ggggttcggc cgaatccgcg cttcgacctc ttctagaagt tgctccgcca 11340

aacgcaacat aaagggttac attcagtgca tgatgaagtg catatgtagc gtcttcgatt 11400

aatgcctctg ccactatttc agaacttaac ggttttccat gcatcaacgc tgcatatata 11460

gaacagagtc cgcgctcggt cgtaaagcga catacgaaca tcggtctcac acgatcctta 11520

cgtgcgttac cacaaacacg attcggacat ggcaaacaaa aatctacaag tattgacgat 11580

atgccctctg gtggagcagc aaaatcttgg cacgtttcat tggcaccatt tgacccatgt 11640

tctagtattg atctaccgct ttcataataa ctgacaatat agatatcgcc caaatttacc 11700

tgcccggcca ttagcatgag tgaatgggtg atgacacacc catcagttgc atagaccact 11760

cgtatggggt cgttttcgat gctcgctttt acaaaatcca tatcatcttc attgtcagaa 11820

aaatcgaggt tgacgtcctg tccttgcaca gccattatat ttaagagcgt ctaaaagtta 11880

ctgtcaatca tcagaccatc agtatcaggg cacgcatcca cacgaacgcc taatcgcgtc 11940

acgcggtagc aaaggtatat agagctctgc cgggaagatg tttttccgtt tggtttgaag 12000

acgagcagtg tcgtcttctg ctagattgaa gacgagcgga acatatttcc tttgtgatac 12060

gaagtttggt ctggtgaaga agcagctgat tatgttagct tttatctcca gcatgtgtgg 12120

tctaaggcgc ggggcggaag aaacgtatga gcctgtctca atgggtgtgg cagatgggcc 12180

atctttgatc cggctaaaag aggccttaga aaacgtcaac gctatgctac catcgccaat 12240

tacgctggaa gatgcagtaa cgtctgcgga tactataaaa cgtctcgcca gaggaaatac 12300

gctcgcacga acataccata tatgccagcg taatttagaa tgccttgcaa aacatactcc 12360

taatacagac aacccaggac tggatgcagt tgtgaatgca catcgcgtta ataacaaacg 12420

cgttgctgat gcatgtttcg cctcgctaat gcaactatat atgtcggtcg ggtgttcgga 12480

tgtgagtgat acactagtgg acggtgcaat taagatggct gcagaaacag aactggtgat 12540

ggcagatgta gctgttgccg agaaagccct cggtcttaat ggcgcacaaa acgaggcaca 12600

aactgagatt attggaaaac acgaagccca aaagaggcgc gctccaaacc cgccggttgc 12660

ctcatggact gaatgcactt ctgaagatca gccagacggg cctatgcgca aatcggtaga 12720

gtatgcaact atcaagaaac cgccgcgtgc aattgctcct tccccggcac cgcgtatgtc 12780

tgttgccaga gataaagcca ttaaggagaa acccgaagag ttacaaacgg ctacgttggt 12840

agccgtctaa taagacctat ctaatatgaa gaggttgtcg tactttaaat aaaaagacac 12900

acaaaatgta cttttgcaat ttatttacta ttgatctttt aaagtcaata aaggaaaact 12960

aaaaagacgt gcgatttaat gttttgtgat ctgtttgaca ttatatccca gttgatccga 13020

aacctccttc tcttctctcg cttgtcatag cctccgtgtc gtagtgaagg gtgaaacgcc 13080

aaaccggtga tggatatgtt acatacggag cgcttgtcga cagcatggca ggcgggcatg 13140

ggtctgaata gttgtggtgg ggcgggatcc atagattagc atcgcgactt atcagaacta 13200

actgggcaac acgatcgcct gcatttagaa acacggtctt acgagtaaaa ttgtagatga 13260

ggaacgttaa ccattctccc tcgagccatc ttgttggcaa taccgttaag ccctttgcat 13320

tcatagagga acgcccaaat atacatggca agacgtcgga cgtcttgcag ttataaattt 13380

gacgtatttt cacctcggta aatgttctag gctgtaattc caggtctcgc ggcaacggta 13440

tatcatatcc agcatcctct agtcgtttac gattgaaagt gtcaaaaaaa ggtcttaggg 13500

ttgacatttt cccacatgaa gaaaacataa tgctaggttt ggcttcttta tcatctggga 13560

ctttgtataa ccccaaaaca acatatcgtg aatgctttac cgcaaacgat acttgtttgg 13620

ggtcggcgat atattgtgta gtagcggctg ctgcatcact tgttatcaca accatggact 13680

cgcattgagt cccgggccaa atctttgcgt tatctggtgg caaacgtagc agacttaatt 13740

gcgtttctgg gctacagttc actgtagcat cgcgtacgga ttcgataaat ttgccgccgc 13800

attcgtaata attactcgca tcgtagaggt caaacaaaat cctgcgttgt atttcctggt 13860

cgtccgatag tttgactaat gctagtcgaa cgctcaatgc atttgggggt attttggtta 13920

tagagccatc gtaataaaca actaagcgga tggtgccgcg gtatcccgca tcgactacgc 13980

cgttggcgat ccgcaggaaa ggagaaccgc ctcctataga atctgatgaa aggaggagcc 14040

ttgtcaccaa aaccacataa ttgttaggga aggctactcg tacaccagtt tctattagag 14100

cgacaagtat ctccccagga cgacagctgt cgggcgtgtc tatagcttta gtgttgtcca 14160

gacggagaca acgcggatcc gtcctatcca cgccacaaaa ccagtatccg ccctcaccgg 14220

ttctgtcgtg ggaaactata gcttcctcca cacagaagca aactggtcca gtcggacttg 14280

tggattcgtt ggagcgcaga tcttgtataa cgcgctttgg agctccacaa tttccagttg 14340

ccatggcttc tatggagaca gcttctgtgc cggctacacc ctcctctgtt atacttatcg 14400

ccgcgttgat aatcgccgca cttataactg gggcgcgtgg agaacaagag ttcgaagaca 14460

ctttctggtc gcccagatgc tctgccgttg gcgtgtcagt tgccttctcg tcaccctttt 14520

ctctgatgtt ctacgtagct ctaggcggtg ttgccgtttc cctattggct ggcgcttacc 14580

acgcctgttt ccgcatgttc acaaaagaca tgtttagggt agattggtaa ttacatagca 14640

ggatttggtc gtagcggcca gatcggtata taagtcacag ggcggcagct actggtcatt 14700

gttgcgtttg gtggtttgtg tgaaagttct acgctttctt ttgtttttta actacgaaat 14760

ggcaaataga atagatcgac ggcctgagca ctctggtagg aatcatcgta atgtgtatga 14820

ccaatatgat ttagacgatt atgactatga cactccagaa gaatactccg accctggaga 14880

ccataaacca cgcagacata gagccgatgc aactaccccc catgtaccaa ctgccggaga 14940

agcaagacga cgcagcgtgt ctataggaag atcggctgat aagcaatctg gaaggcgcat 15000

ttctgcggct agctcttcct ctcaagcatc gaccgatagt tgcagacaac gcgcaccacc 15060

tggcgctact accgtgctaa agaaatccct ggctttcagc aatactcccg aaacgagtac 15120

aactcaatgg aagacaagta cgatctcatt caataagcgt atattttgcg aggcaatagg 15180

agctgtcgcg gcccatcagg ggagacttgc tgcagctgca ttatgggatc gccattctcc 15240

gcgcaataac gagagccttg acgctctact ggaaatagct atggtcaaga ttactgtatg 15300

tgaggggtta gagctgttgg agattgccaa tgaatacata aaggcccatt cggatgagct 15360

ttctggccag acccctcgtc aagcggcggg acggcgggat gacggtcata ccgagactac 15420

gggccgtggc cgacgtagtt ccgttgcccc ggggggaaaa gacgcacagc atgcaagggc 15480

tccgtccagc caccgtaaca gttcaagaag accacaataa tttgaaacct aaacaagcat 15540

cacgagtgtg aaagtgttga tgacaaaagt gccaaaattg ggtgtgagct gtgtataaaa 15600

taaacctctg aacctaaaaa ttgcagttct cctttgtgct ttttggaggg aggaccgaat 15660

agttctatta acgtgttcgt attattaggt aaataggtgt gggtgtggat atggatacat 15720

ttgatgaact cgacaggcta ctggctaata ttgacgcgta ttcagcaatg atggcagatc 15780

taagtgtaga agagcagcgc tgtgcgggtg aaggttatac tgcacaggcg acggacttaa 15840

gtgcagaagg aaggagcttc ttggacgacg tttatactac acagatgaca gaactaagct 15900

tagaagggaa gcgctttata gacgacttcg acgacacctt gttagatgat acatctatgg 15960

gttacgctaa tggtacgaat aattttaaat atccgtctgg cgttccagtc tcaccggctc 16020

agttttcaaa gcgcatcgcc ttacccccac cacggccgtc ggcacctcga gtattatacg 16080

tacgtttact gcgcgaactt gaatttcctg aagcgtctac aatactatca acatttgaac 16140

ttatgaacat ggacttattc tcatgtttgc cccgcaacga agacctttat agaaatgctg 16200

ccctactatc gggatctgtt acagaggtaa tagaagcatc tgagattcct atagacggag 16260

aaatagactt aaatgcgcat ggatctaagc aattacctat accgccgtcc aatgaagggg 16320

aaataccgaa ttatgttgaa gcagtgcaac agttctttct tagtgaacta cgcgccagag 16380

aggaatcgta tttgaagtta tttctcggat actgtagagc tatagtaaac tacataaagt 16440

cagcagcaac caggggcgtg aggggctgta gaataatgga tcggaaatct gaagcgaaca 16500

agagaatgcg tcgtatgatt gcggatagat attacaggga agcagcgcgc ctggctaaag 16560

ttctgtactt gcacctatac ctatcagtcg cgcgggaaat atcatcacga ctatgcgcga 16620

cgcagatgca agttcagaac gtctttgtct atatgcgata cgaatggcag caatatagac 16680

aatttacatg cttatttcat cctataatgt tcaaccatgg cgtcgtctta ttgaatggag 16740

aacctctcca ggcgcagaaa ttgagggaag ttaattacat acggttcaaa cttggtttgc 16800

ctgtcatacg ttgtggactt gtcgaggaac atgataaacc actggtcgaa gaaccgcgtt 16860

tttacggcaa tttgccacgt tcttccggat ttttgactga actaattaga agcaagatgg 16920

aagcgtattc agagcttcgt ccaacaagcc cttctagcat ttctaatgac catacatatg 16980

ctaaacaact tgataaagtg aactttggaa gcacccgcga agcaatattg gatactatag 17040

acaccgaggg accgttgcca ggggacccta taccagcact gtccgtttct gtcagaaatt 17100

taccagcgga aacgtcgttc tcgccagata attagcctac ataatagtta agtacatgtg 17160

actaatcacg agatccaatt attcaatgct atttgctaac gaattaaata aacgcgtaaa 17220

tcacatttca tacgtgttct gttatttttc gtttctaaag tgcgcgggtg acaatagcgt 17280

tgttatttgg agcgacgcgt cgcggtaact ttatttgttc agacggctcc agagccgccg 17340

cacctatcaa ggttaaaaac gttggtgctg tatagataat tttactagag aaaggaggat 17400

tataggttca agtagaatgg ataaatcacg aagacagcgc cgtaggtcat caacgcccaa 17460

tgggccaatt aggtcggcca ggaaatcgga ggatcgtaga acctctaccg atccttacgg 17520

tgccggaacg tcaaggagaa gtggtaagag acgtacactt gacaggactg aactgcctaa 17580

ttattttgat gaaaacgcca gacatggatt tgagtctgac gatgatgaga tcgtaactat 17640

ggagttagat tctggtagcg ttgagccgcg taagctttcg aaggacgccg caggcggcct 17700

gtggcaattt atcagccatg cctttggcgc gtcctcctct ggctacgagc aacatcaagg 17760

acatactagt tcatcggacg atgaatacat agagagaaga cggcgcatta gaacttgcag 17820

agatgtgccc atagaaaata cacattctga agaagaagac attagtgaca atatatcacg 17880

atctaggcgc gagtcatcta aacgggcgcc agtacttgac aaagctcctt cagtacaaac 17940

tgaatctcgc ccggcctaca gacaccgcct taggatgggc gataggacag atgatacctt 18000

tttaatagga cattcagcat catgtaaatg tggttgtaga gatagagact cttgtttact 18060

cccaaacgaa caggtaagcg agaacgatta caatgacgaa atggacagcg ttccaatgga 18120

agacagcagc tctagtggga cacggtcaag taggcggttt gttaaaccgc gtgaattagc 18180

tctagaggag accgaaggca gtcttgacat ggatatgttt aatcgatacg aagccgagtc 18240

atctgatagt catacgttat cgcctaagat gttgccatca ttagacggtc gtatattgct 18300

tggagatttg ctcaaccgtc taccatttac agaattaatt agtcctgtta atccggcaat 18360

agttttagga gaggacagat tgttttttat gaggaattgg caatatggtg acgtagtcgg 18420

atggattaat aacaagagcg acgaaaacct accaggggct atgtattttg gacgtccgac 18480

aataacaggt gcaatgtacg agcgtacgtt gatacaggca tatttactag tccacagttt 18540

aactgatgag tcattacact attctgtgaa agcctcggcc attaccgctc catcggcagt 18600

atttttccta ttagacgcgg ctgtattagt ggcaaaaaat tgtcatgcca ccattaggtc 18660

actagatcgt ggaagcttac tgaagcagct accaggacat gtatcaacgt tactgcatgg 18720

cggcccagta agtccagtta atgggccgac tgctgctatt atacgctctt catatggatc 18780

ggctctttat tggcccgaca tacgtgctgc gttcgaaccg gtagtaagac ctatatgtag 18840

atatgctctc gttaaaatgc tcgatgggga cgtattttta ttctcagtct tcaacccact 18900

ggacaagtct gcgtcagtaa atgagttgcg agtagcctcg ctatgtctaa ctctaggcac 18960

aatggccgct gaggcaatgg tcagacttat ttatcttggc attgggcgtg cattacaccg 19020

ggatggcgat gataaagtat tccagagcat tacagaaata atggaaacga gtcttttccc 19080

cctcggcacc gagcttgctg ccatgattga ggaagatgct ttgtatgata ttgatggagt 19140

gcacatagag gacattccat ttgcaactgc ggcactaaat acatatattg cagttcgttc 19200

gtcagcaacg gagttgcttg gagaacatat agaggcttat gggtcaatcg aaagttacga 19260

tgttcctgca actgccatcc attgtatact ggcgactaca gttgtattac aacgttactt 19320

agggcacttg aatttcctac tctgtttcat cgcccatcat gcattaaatt gtggtattgg 19380

aaacattact atacgcgaaa acacattgtc tagatacaag tggttgatga cagtcacgtc 19440

atcgttatat agacatgtta ctatggaaga atttttgaag gattacgaag atgcaatgag 19500

ggaactttct atctctccga actctcatac accatccgga cgcggtgagg gaacaattac 19560

aattctccca catacaccta cactggattt actacgcggt catggaaatg gaagagtacg 19620

ctccagatcc cataagtatc ttggtgtctc cgttccgttt gaagaagcta tcaatgaaca 19680

gcgaattctc gtacttggca aatcaacatc aaaagcatta aaacgacgtt tgactggtgg 19740

gagggtacca aaatctgggc ggagagagtt acattaaatc gtcaagtacc taggccatat 19800

aactagatac tattagttcc acgctcaata ttggctacgg tgatgtcgtc cagggcgcca 19860

tcatactcct tgcgtatccc tgaattacgg agctcgcgat gtggactatc tgggagcgat 19920

ctaatagcac gtaggctgcg cgaaggctgt attattcata ggccccagga tgtatcttta 19980

tcagctctac gagcattgga ggcggaagcg gagaaaatac taccatttca gctatttgcc 20040

acacaacgta cggaggtatt actaaaaaca agatgcaaca acgtcccgga gtctataata 20100

atctcgaatg ttatgtccga tacgaacgga gcgtacataa gcaactatgc atccgaagcc 20160

aaagcgacta taaaggacac tggattaaat aagggagctt cgtggagcgt tatattggca 20220

cgttattgga aatatctccg ccacagcagt ggtgcagaca tcgtcgttaa tagctcaaca 20280

tcaagtacac gtgcattgtt ggggaatgtg acacctatac ttatcctcag atctacattc 20340

gagccaaaac cattatctaa acgaccactt aaggggaaaa aggtaacagc gtcttatctg 20400

gaacaattaa cagaactaaa gtctaatttc gacttagtcc aacgatacat gttttacatg 20460

cgtccagaag atccgatgca ggctagcatt tcaacggaca tacgactgag ggaaattatg 20520

acatataaca gtatactata ccgctggatc ttatggttca tggatttatt tgacagtaac 20580

gttctgcgcg caatgggata tgtaaccggt accaacggtg cgagagaagc gcgacagcct 20640

gagatgcttt ttggaagaca tctacgcagt ggcccaggcg caactgatac atctggaaat 20700

gccgtcgctt tgacttggtt tacacataat gtattatctt cattgctgaa gataagtgtg 20760

ttatggtcat cttcaaagtg gcatagtgga acaaatggag tgtcatctgc catcgttgca 20820

gcggttgaac ttgttacaat ggttcaccat cattgtcaat atctggttaa tatgattatg 20880

gcaggataca tttgttggat taagggcggc gcgaaggata ctgttttaca atctgcattg 20940

cgttcccaag gacgtttttg ccactttatt ggcagactag cccctacgat gacagtacat 21000

tcgtggtctg aaatggaaaa gggaacgcga gcgtggttca aaatggctca cgctcgctca 21060

attattactc atggtaaacc aactaaacac tatctaacca cagtagggtc agttgatgac 21120

ccgtttgcag atacggtgga taactccgac gacgatgaca cagaaactct taacgatgga 21180

tatattacac gatatggatc atccatcgta cggtcatcga gagcgagtgg acctggtggg 21240

ccttatcgtg gaatactggt caaacaatca aagacggata gtgaagaaga tttgaatcca 21300

tatttacata tggattcgcc caaatcgcct ctgtctatat cttcttttaa atcaagggcc 21360

aggcacaggc gcacgtcttc gtcatcatct actaagacaa cgcgcaatga cccctcatac 21420

tggtttgcag acggcgataa tctattcagc gttgcatcga atggtcatgg acgtattcgg 21480

gagaaaacat attcattaaa cggacaatta aacacatcaa cactcgacgg accactttct 21540

ggtccgggaa ctagcgctga tggagaatat gtagatatgg gccagggggc aagtcgaagc 21600

aaaaactgtg cttcatgtga tacaactacc gctaccacca agacaaccta ctcggaaaac 21660

gctaaccatt gcttcgaata cgagactaat aacgacaccg caacgggtcc taaatgcgag 21720

tcaccagacg atgtttatga gactatagaa cgcgcttcat ctaactacag ttatgagaat 21780

gaaactaagt ttgcaaccgt tgcttctgat ttgggaagga atcatcgttc aatgtctaga 21840

acgagttgcg cttccccaaa cgtgttgaat tcttaccacc acggatcgag aacagacggc 21900

ggctcgcctg tacaattagg ggacgatgaa acggtgcaat gtcaagttgg agagacgacg 21960

gcggcgttca gaccgcgggc atattgcctc ggtaaacgtg atcgagagcg cgaactagct 22020

gcagtgcagc gagttatgaa tagactgtca tttgggaaat aattgatggt ttggcgtttg 22080

gtttatacaa acataagttg tatcctactt tttaaatctg gtttatacaa acataagttg 22140

tatcctactt tttaaatctg gtttatacaa acataagttg tatcctactt tttaaatcta 22200

acaaattggt aataattgat ttagttttaa ataataaaca atctgattca tccaaatctc 22260

gagttttgta gtaattttat taagatgggt agaaacacgc atacaaataa caagtcttac 22320

gacaaaccaa agacgcgccg gacatagcga aacattccag ccacagagcc aagtggtctt 22380

ggtttctgac acacagtcgt cgcagtttgt gaacaaatat catctttggc gccagctgca 22440

tcgattctta aacaatgtag tccgttcaaa ttcgtccaaa ttggggggag gctttctcca 22500

attgacgtat tgtttgcata ttcgccgatg gctgctagta atgtttttgc attagcaacg 22560

gtaattggtt cggcatcaac ggccgcgcat agtcctatga cgtcctttgc aatagtatta 22620

agcctgacgg catacacgca tactgtgtct attcctatcc aaccgtggtg acaagtgctc 22680

gtatcccacg ctgtagtcgg tatcgcaaat acgccgacaa aaaagcatac caatccgaca 22740

attaatatac ccacgacaat accgattgtg attaggcgtg ctgttttagc acacccaact 22800

ccatggcggt catagcaaat tggccgcgat ctttcctctc ccaaagacca gctatttcgc 22860

cgcgtgttaa ttggctcgcc attaccaaaa ttccatccgg tttgcggtac agcactttcg 22920

cgcggttcgg agactgaaaa ctcgtctcta gaatgcacaa caagttcttc ggccgacatg 22980

ggcgccatca ttgttagggt gatccgcccg caatacacgg attagccaga tggattgatt 23040

gacgggcaag tgtccagtta tgtatcctaa acataaggtt taatcgatga gttgtgtgat 23100

gaattagcgc cacaacggtc ggtaacgtgt cattctagtt atttagtaat attatgattt 23160

aatgcgtcag attatcttaa taatggggtt ggaaaatgac atcactgatg aatggcctct 23220

cagtaatgtt gccccattat gtatatttcc accggacctt tatttaaagg ggcagtgtta 23280

tcaccaaagg acatgtcaaa gcaagggaga acctatcttt tacgagagtt taggttatct 23340

cgcctcgctt cgatatgccc aaagcgtaca gcattcattg ttctgcttgc gaagaatgta 23400

tttcgccgat tggctgtgat gcggatttaa tttacggacg gatgcttaga gaatttgtaa 23460

agcgacaatg gccggtcaat tggttcggga aattaattac ttcgtgtgta acacgtaatt 23520

cgatcggcgt cagcggattg gtggcgcatt tttcgctaga gaggaacgct gtaccaatgt 23580

ttactacatc acgcgttcca tggcaactca tcaagaaaaa tctatcatcg cgtattcgtt 23640

tagctgcatc ggaagaaaaa cgtaagttaa gaaacgagcc gtactgtcct tggggggaca 23700

attattcaat gacacccgta ccgcttggcg gcatagacgc ggcgttggac atacttcgac 23760

gcggtccatc agcgcatttt acgatacaag tagattgctg tcctatcagt ccgtatatcc 23820

tacaattggt cgtatttaat ggccatatga ctgcgctgtt gcatgtggat actatgcacg 23880

tcagcgataa atatggcatt ggatggctat tgaccgaggg cggaatcttg gcacattatt 23940

gcgcaaagcg acttggcgta tttgctgatc gtttagcatt gctgatagga cttgcaatcg 24000

cgtctagtaa acgtgaatgt atgcgcacaa tcaaagcgac gatgaatact actatggtgg 24060

tatacccaaa ggttatcttt aggcgttttg cccgttcaat agacgattgg agaacaaacg 24120

atgtaacaat atgtggctat gattcctttt gttcggatac tatatgaagc tttggactga 24180

atgtgccgaa caatctgtat agacactgcc ccatcatttt tgaagttgtg ctatctataa 24240

actcaattaa tttgaatgac tgtagaactg tgaagcgctc gaactacact accattgtta 24300

gcgtcccgaa tataatgaat gctatttaaa acgtcgttta tttatcaaaa gctttattag 24360

acattttata ttaaaccagt attcaaataa tattgtctgc tttatctcgc gaataataga 24420

aacaaacggc cataattaat attatcccgc cggttaaaat tccagccgtt aaaactgtaa 24480

ctatgatcat gatcataggc ttcccctcgg aataggggga cgaatcataa agggccgcat 24540

cttcaaatat aggcagttcc tccggatagc cttctacgcg acatgtgtac tgtgtcaccg 24600

tgccagtgta ttcaagcggg taacgagatc taatattgac agttccggga tgatcgatgc 24660

attggcctac aacttccacc attccgtcct tccaaatgtt ttcttttagc tctcttccaa 24720

ccatccattt aattccaacc gcagctgtgt cggcgacgca tcttgcatta catactgcat 24780

ggcccgattc gaatgttaag ataacgtttg gcttagaata aacaaccgga actgctgaag 24840

cattaaatgt tcgcgtctcc ccttcgccat tccatatagc tttacatgtg atctctgggg 24900

gcatagaatc aatagaactt ccgagaagaa gcgaaccgat caaagacact gtgtcttctt 24960

cactttttgc ggtagaataa gtccgcactg agtttatagg caaattggtt gaatcatcaa 25020

ctacccacgt catacggcct gagccggggg ggaaaaaatt ttctaatata cacgttgccc 25080

gatatcgttg gccataaacc atcggatgag caatgacaga gatgccggat ggtatcttta 25140

taattttcag tgttattgct ccgattgctt ctgtccgtct ttttgtctta ttagtattac 25200

tttcaacaaa aggcgtagac aacacccatt catacaatcc ctcgtctaga gcagtcggct 25260

taaggatagt caatggaaag ctagagtctt cggtcggttc aaacccataa tgtttttcat 25320

ccagtccatg caaatcgtat tcgcctaggc cactatccgc attacttggc atcgcagtat 25380

agatgattct aggttgagct tgcttagaac cgacgggatc atactgcatt ccgttccatt 25440

cgactggaga caataaatcg actgcttgtg ttggtttgtg ataaaaagta acgaccattg 25500

tccaattctt agcattggtg gcatttcctt taggttcgca tccgaatgtt atcttttccc 25560

catgatatgc cagttgtttt gtcgacgaac atgtcatctg tgcggcgttt tgcgatcttg 25620

taattgttat tgttaacagt agcgataccg agaaggcaac catcactaat ggccccatga 25680

tatagaatcg ttaagtattg ttacaacttg atgatgtacg aaggatacac cggccgaaga 25740

ctatgtgcgt cggctaaatt gggaaaaaag ctcaacacga cacttttata tgcaatccat 25800

gtctttattt ttaacgaatg ttataccgta ctgttgcagt cagtgttcga tagtaatggt 25860

tttattcacc gacggacacg ctttttatga gctgtaagtt acagttagta acccatttgg 25920

taaatacggc gaacgtaggt aattccaaga cctagcgcga ttgaacaaaa aagtaatgca 25980

aacatgctac gtgctactac tgaaaatgcc agccccatga gtccaacgca tcgacggtgc 26040

agcaataata tacacaacgc tacttgtgaa ctaacggaaa atacgtaact atatattagt 26100

tcctcataac ctctagtcac taatatggta gagataacct gtagtagtat atatgttatt 26160

ataagtacac gctctacata aatggccatg gaacatgtct tatgtggtaa tataaacaca 26220

tttacgagat gccccaacag aactgctaat gcagtagtct cagttaactt aatgattggt 26280

atcgtaccac catatgggaa ggtaagtact ccgtataaaa acggaattgt taggattgaa 26340

aatagtgtat aacatgaatg tcttttccgc cgtttaagga catcaaaaat agacacgtcc 26400

tcttcgtgtg ttccaatgtg cattcgaggt agtccatcgc catttggtgg aattgaagcg 26460

ggctgaaatg ctactccgaa acccatatcc gcatatgtcc tcatcatagc aaaacgcgct 26520

aatttgtaat gacatgtgtc acgcattttt gcgccatagt ccttcaaaca gcccaagatc 26580

attatagcgg cgccaaaccc aatcgctatc cacacattac ctctaattaa tggggttaca 26640

attcccaaag aaatgccaag agaggcacta ataaatgtag ctatcatatg cggcggtgct 26700

ccggtcgcgg cacatgaaat gtagtatgcg aaaactggcg taccgaatac gacagataat 26760

actagcgccg ttacatatgc tatgttaaac gcatgcatgt tcgagtcgat cggtatctta 26820

ttccgcaata attctgtctt gacctccgtt cttgtcagtc ctataaccaa ggcggaaata 26880

catgccaaaa tttgcaacaa cttaaacgtt aggcacatca ttttcataaa ttttgatgag 26940

tatcgtaaaa tattaagtat tgctatagcc aatagcgcaa ttgtggctcc ggccaacgaa 27000

atgcgattcg ttggcataag aattgttgtt gtacatgcag caatcatagc tgcatgaaac 27060

ccgatcatga atacagatat agtagatgcg attgtacatg gcccacaacc atctcgcgat 27120

gctctacatc tcgatataga actagatgtc atcaactgtc tggacccatc ggtcaatagc 27180

ggcgccatcc ctgtatttgc ggcgatcggt gcttgcggta tagcagtatg cccatttctc 27240

tctaaattca tgctgtatgg aggtagggtg catgcagaat tgccatgttg gctcgcttct 27300

tcgtaaggag gcggtggcgt tcgcggtgtc tcgccggtcg tgtccgtaac cgtaggctgc 27360

acttcggcca cgacaggacg ctccgtcgcg ttcgcctgct cgctcattct aacgctattc 27420

tataatctta tacaagagtt tatacgagcg ctagagacgt cccatttgaa tgaaaaatag 27480

tctaagaata agacaagctc cagacaactc tcaatttaac tcttttatta caaaagcatg 27540

tatttacata ttagtcacca ccctcattga atatgcgcaa tttacatact gttgatatca 27600

atctccattg taattctact caaagtatat tataggtagg gcttcattcg ttttgcattt 27660

aaacagttat gttgcaggcc agtgtccttc ctttttctct gcactggcaa gtccgttatc 27720

ttattaagtt tcattgttcc atcgatggca caaacttcct cgatttcttc tatcgaaaga 27780

cactcatatg tgcagaagaa aaagaatgat gtgcggcttg gttcgtctaa gcatactctt 27840

agcatcggtg tttgggtcga cgtataaaag gccaatgtaa aaccagatga gaccgacttc 27900

actttctgca tcacgctgtt gaatgcttta atgtcaccca atgccataca gaatggtttc 27960

ccggatccta ttccatataa gcaatcggaa cgtgcacgtt ttgtcacctt tccgatcaag 28020

gccttagtgc tggaagtcgg acgactaaac ggataatcat ccatatccac gggcgttatg 28080

tcgaattgaa ttatcccata catagtacca atacacaacc ctccctcagg cttcaggcaa 28140

aataccaact cttctggttg agtcttcatc gaaagtccta taacacgttg aaattgtgac 28200

ttcgacagcg ttacggtcgt atcggcattt tgtgaaggcc aaaaacaatt atacgatacc 28260

agttcgtatt taacgtttgt catatcccag acatgtccgt cgctgacatt tttataggta 28320

attgtctggg ccatgtcgcg tgtagggtac gtaccatgga agttaaattc caccctcata 28380

acttcttttt tcttgtctgg cctgaatgcg tccaacagag ttttgcgacc atctactaat 28440

gccaaaaaca tagctgccgg tccgttccag atatattctg aaaacttggc tgtagacagc 28500

ggcacgaaga cgcgttgtgc ctcaaccgat gcatgtagca ttactccttc gggcccgaaa 28560

gacaggaatg gatcctttac tgtactcaga aatggcttca gcatagttat catagcatgc 28620

aacttatctc catggacgac cacctttgta gcagttgggg cagatgccga tgtttcccct 28680

tcgtcgtcat gattttctgg cgtaaatttg ggcgtagcca ttattagaaa cccttagaga 28740

ttctagtagt ggtagtagca gatgcgatcg ctgagatgcg atgtatacca atagcgaatt 28800

ccctctcaat cagccgtctg cggctgctcg cggccccaaa agcattttcc gcgggtttta 28860

taccacgcgt cgttacccgc atgaaatgta ggcggaggcg cttaactcga attcgccgtc 28920

tgcgcaagtt ttgcttcata agaaccaaga agtctcatta acattttgta ctgagaggcg 28980

gacagatggt tcccgaatac tagatagtgt ccgatgaaaa tacatgagcg ttttccatat 29040

agtcatgtat ggcctagata gtgaagcaca ctaatcgggc ctgcgcactc ggacgaatgt 29100

tctagaacat ggaaccattt aaactacaat aactaattga atatgtgtat ctctgtcaat 29160

atatgtcgtg gagaaatgct actatatatg catattacaa aagcaaacgt aagttaacct 29220

atattgaata aagcatgaag tcgacgatga gccgacatga tgaaatgagc ttactccatt 29280

aggacaactc atccaacgag cctattatag cgccgggcaa ataatcgacg gatagatttc 29340

ttaacgcacg ccaccgggtt cgaaacgtca tgcgcatatt aagtacttcc tgttttgtcc 29400

caatggaaag cgtcgtagtg ttcgctttcg aaacgctttg ttctcattag ggaggaggaa 29460

atagaaaagc aaacgcgtct tacgtacatt actaggtact ttattcttat cacggaccgg 29520

tagtcttagt attcgctttc caactctggc tatttaacca tccacattat atctaggagg 29580

actatcaatc tggccggcta tttgaatcga tattgcatac gacagcaacg atacgattcg 29640

gctgagaact gcgccattcg gttacacggc ggccgttacc atttactgcg cgagacacat 29700

cagtacggcg atccgattac gggacaacag cgccgaaacg taagacgaca atacaatggg 29760

gctgtatggt tgtataagct ttgcttattc gcacggactt gtgaagaagc aagcttttgt 29820

aatgccgcct ggtgtattga ctcccgttgc cgtagatata tggaatgtaa tgtatacgct 29880

aatggggaaa ttctattctc gccaggtcga ttgcgacgac agcgcgctac taaacgttaa 29940

atgcttgtat atggttctta gacttttaca taagcgatct tgttacccaa tatttgtttc 30000

tgatcgcgcg tattatttca acaagaaagg atgtaaaggt gctaaagcaa tatttgccag 30060

tactatggca gccgaggggg gaaccggccg gcttggtaga tttgaggctt ctctggatgt 30120

ctggctagat gaggatgaag cattagaaac tgcatgtaaa gatgaaggag ctatcatttc 30180

taatggagaa ttagcgcccg tcggtacaga ttgtcaatcg gatgaatgcg tacaggaagg 30240

cgatgtatgc caggagtatg aaataatgcc gagtctggtt aacgacttaa ataatgacac 30300

atcttacaag caaggtcgta ttgtgacaca aaagcctaag cgatatggaa caagacctga 30360

catgcccaaa ttaaaccacg gaatatgcat gagtattata aaaatgctcg gctttccata 30420

cgtggatgcg cgttccatgg aagcagatga tgtttgtgca aacttattcc atacaaagac 30480

tgttccatac gtatactcta atgacggaga tcttctattg atgggatgcg acgtaatact 30540

agatatgagt caaattttcc ccccaacgct tcgctgtaaa gatatattaa atgagatggg 30600

gattgactat gctacgtttc tagcgaaatt tgttcgctgc catactgatt tacaccgcta 30660

ccctatactg aagtctgttc atgccgtgtt gcatgatatt aatcctactc attgcgatgg 30720

aaatattgaa acaatggctg aaagaagtga gacgtcggca caaaaatcat cctcagataa 30780

ggctataaac tgcgaccgca gtgtatacat tcattcgtca tcacaaaaga aactcgaaaa 30840

gtatgaaagt gcgcgtagtt gttccaactg gcgcaaaccc gtgattataa atgatccaga 30900

gtttgatgat gaccaaaata tggatgaaaa ccggcgacct atagatattg tggctgaggt 30960

acgcgcggct ttggatatga tgcccgtgcc gcgtacccgt cacgaagtgt tggaggtaaa 31020

atttatacgt catgtaatgt ctttggtcac ccccagacaa tatggaccgc acttgaagct 31080

actcaagcgg attcaaataa cgcaggacga aaggaacgat gaaagtcttc tcgactttct 31140

atatagtgtc ttagctcaaa aagatgccga agaactatca cgtttaatac taaatcgcat 31200

tccaccccca caagattata gaactgtatt aataaagtat tgggactaag attaactgtt 31260

gtaagagttt ttatttaaac tattaataga ttggtaatca agagtttact gggaacagaa 31320

taacatattc gaatatataa gagtagaaaa ttaaggcgta tagagggtta cagatcattg 31380

agtacagaac cagcatatgt tgtattgcgc cgttcaaaga aattggtctg ctgactggtg 31440

gccataaacg ccagagggaa atcatgcttc ggtggcggct catcaaataa cggggccagg 31500

ccgatatcac taagaagcct atctgcgttg aagcgcacgt agctgcatat agcttcggtg 31560

tcgatgatag cactgtcgct aggtgctcgt gcgcgcagga attcacactc caatttcacg 31620

gcctcgctga atagtttgta aatgcggcat gtcgagggct tcttgtattt ctgtagatgt 31680

ttgttgtaaa taacacatga agcctttgta tgaatcgttt catcacggct tatcattgta 31740

ttggcctggc aggtgaccac aaaaatatta cgtgtgcgca tatacgctat ggctgcgaac 31800

gacgctgcaa aaaatagtcc ctcgattaag atcatcaata tatacttttc tggtatatct 31860

tcacattctt caacacgttc ggaaatccat ttcaccttgg atctgatcga ggtttcacga 31920

atagctttct tgacatattc tgctctggcc gttgcatcat ttttaaacag aattagttga 31980

ataatactgt atacgcgcga gtgaacagcc tctatcgact cttgttcaac ataatagtgc 32040

tgaatgtcct tttcttcaaa cagccccatc aggtttccga ggttacagtt gacaaggtca 32100

tcagcagcag aaaggaacgt gaaaatgaat cggtaaaaat caagttcgga tggtgtaagt 32160

ttatccacgt cggcagcatc atccaaaaac actaactctg actctagcca tctattaact 32220

atactaagag agcgcagttc attcatctct tggcactggg ttgtataaaa gtatgtttcg 32280

ggcggtccat aattaaggtc atcatcgtcg tccatcatta attgtcttga tggttcggta 32340

gcttgagatg cagaaccata gccggaatcc gggctcgggg gatattggtt tgcgttaagc 32400

acggggccaa tagcgtcatt ttgttgaagc tccatcttat gttctattgc tgcggtagga 32460

accgttgcaa atttagagag cgcagctcgt gcacacagac tctcctaagc cacaaaaaac 32520

gccgttgttt gtggctttgc gaacgcggca gtagtaagca gccgtcttaa ggccagtttt 32580

ataagcatga aggagtagct tgacaatgct ggaggcgtgg agtacgccct ctgacgtttc 32640

agtaatatat aatgaggttg attgactgtg gtcgacaaac ggcgccctat cgcgacacat 32700

gttgagtaga tctatctgat tatattcaaa aacagtctta tatttcataa ggctggcgca 32760

ctccggccga ttcccaaagg ctgcgacagt tgaccattgg gccgattcca aagcctccag 32820

agcctctacc ctatctttct catctgggta tatggcgcgg agctcttcca tcagtaaggg 32880

attggggcga agaatttcac ccgttgtcga aaccttattg aacagattgc taaacagtgg 32940

cgtaaatccc tcgctcactt cagttacctg agacgatgac accgttggca ttagggcgat 33000

aaattgagaa ttgtaaagac cactagtttt tacacgctcg cgcagctcat cccattcata 33060

ctgctcggtc agctccacat cttcccagcc atcgaaatga aaacgccccg atgagtatat 33120

gctttcatcg aaatcgctga attttggaag gccatgttca caaagctcgc aacttgttgt 33180

cattgcttcc agcagcatta attctgcgat gcgtttgttc agctcgcggg cttcatcgca 33240

taccatatca agcccaagac gcaaaaatgt tgtatgtagc ccctgaagtc ctataccgat 33300

cgaacggtga cgttccatac cagcgcgcga tttttcggtc gggtagtcat tgcataacat 33360

catcgcgttc acaaatattg tggcagttct gacggccctt ctgagcgtgg tacaatcaaa 33420

tcgtcccctt ccggcccgat caacgactat acatctcgat aaattaatgc tcatgagatt 33480

gcaaacgcca ttggaatctt tatctgcttt atgtactatc tcagtacaaa ggttagaaca 33540

gctaagtgca tcgaactgtg tatcgagatg gtaatggcga ttaaatgcat cttttagcac 33600

tataaacgga ctgccagtag tcgccgcact tcgtacaata ctatacatca tctccctaat 33660

tggtatgcta tcaacaccaa aaccgctgct ttcatagtat tcatattcac gttcaaagcg 33720

atcgccatat agtttagata agtttgatgc tctgtcgtca aaaaatgtcc acattacatc 33780

cttctcccca ttaaggtacc gttcatatcg tttgaacagc aaatccggta cccatagcgc 33840

actgaacaaa tgatcgcaac gcatagcttc ttctggggcc aataaacctt tcatggacaa 33900

aacggctctc gtgtcggcat gccatggttc aatatagaca caaacgccag taggtcgtgt 33960

gctatcagca ttaattgcca taacgagaga atcgatcatc tttaaaaccg ccatgagtcc 34020

ttttgatcgt gcgttctggt ttaatgtatg caatgctatt cccacaccgc cccttgactg 34080

taaaataggt gcaacctcat tcaaaattga gcgtactgta tcatcgtttg tcgtggtgcg 34140

ttgatcaatg agatagcagc tggctgtaga acctacagta cggcccatga acatcatcgg 34200

cggtgttgcg ggaacaacta attgtgaact aagactccaa aaaaaaactc cgaaggccga 34260

cttccatgtt gctctcccgc gcgttagtac tctaatcata tctggtgttt taaacgctac 34320

agttgccgca gctgccgcca atctggcgaa aaattgccct atagattctt tgcctcctcc 34380

ttcaaggtca gctaaataac tctcattgta ctttaatgcc gattgtagtc caatgtccat 34440

aaatgtttta aatgaacccg tgtaaaatcg tacgattgtc gtttcgacaa aatccaagtt 34500

tgcttctata gcaccacgta catcagtttt caatgccagc tccttcgaat aaagccaaca 34560

cttaaatggt acttccttag taaatatacg aagatgtatc agacgaccgc attctatgta 34620

caggcgttcg tcgaaacgcg tcttggattt tagtttgttg accaggaccg taatgtgctc 34680

cccaatacga tctagttttt ctctggtatc catcgattga tcatcataca aaccaaagta 34740

tgcatcgttt ggcagatgtc ccatctgatg taaccggtct tctattaccc ttaattggtg 34800

ctgtatgttt cttaaatgcc gcgcgcgcca ttcgccagta gatgttgcag agtcgacaat 34860

agattgatta aatgcgatgg atggccaata ggataaattc gccataatgt cgccgcgttc 34920

gtgtaccaac ttcccagccg cgtcgattat aaagatatgt gttgtgtaac cccctcggcg 34980

ggagcgcaat tgttcaaaga acagattgcg cgttctttac agactcattt gccttttatc 35040

tacaatgtag acgggcggct gtggcgtttc cccaataagg cgtggtttat tattgaaaag 35100

gagccagata cgacaagaag tagaaagtag ctttatcttt attaaaccgt tatattttat 35160

aattgtctgg cgcacatatt atagattaca aattgcccgt caatcagtaa tagcattcac 35220

gccacggcga accaagagtc caattgacac cttctagcca aataacagga acatcaatag 35280

atccataacg ttcagtgcgt tttaccgccc ttggagaatc gcccgtcatt attccgacgc 35340

ggcgataggc tgcatacata caactgtcaa tggtcggata gccacgtata tccggcaccc 35400

agttgaataa accgtcggtg gctctcggag caaacaatgt cgccctagga catcttggag 35460

cattgatatt ggaatagagc tgtaatagtg ggaatattgc attatctgta gccggagcac 35520

aaactgcacc ctgcattccg cgtatagtat tttctgttcg gagacggcca taaacatgac 35580

caagcccctc acgtatgcat atatcattca atgtactttt aatgaagtac gcgcgtacac 35640

cctcacggcc caaacgatga gtgtagacga acactaaata taaaaaacaa acaccgtcgc 35700

cagaaattct caattcttgg tcgagatcta gaaatgcaca ggctggaata tgcacggacg 35760

atctaggatt tgaagattta tccgtcttat aagactcctg tattccagat gatactgcag 35820

taacactggc tatttctcgc tgtgatatgt acgataataa tcctcctaat aaattaaaaa 35880

ccatatgcgg gtgtacgtga catctaacca tagctgttaa tgcttcccca aacctatcca 35940

aacgttggct aacgcgcctg cttccatatg ttgcaatgat atgcgctctc agggcctccg 36000

ctcccgagcg gtcggggtat tcttcagctc tagaagctac aagaaaggag aatgaaatca 36060

atgatgcccg aacaccattc tcacctgatc gttgtacgga aactgatgcc gtttccgaaa 36120

ggcgcaacca cgcctcttct aacactgtcg gattacgtcc ggggggtgtc gataggatag 36180

acatggaatg aatatcaagt ggatgacgga gtattaaaat tggcattcca ggacgctcgg 36240

attcagggac gcagaaatca gtcaaagaca cctgtctggc caggaaagta agactgcggc 36300

gcgtgtctaa caatggatca tattgtacca atgcgtgcat aatagtagcg gttacgctgt 36360

ctggtcctgc cgctggttgt aagaatgcag tatctgttgt cgattctttc agggtgttca 36420

acatccattc tagaccggat ttcataccag ggttagatcg ccaatttgca tttgatcgca 36480

ttccattcct acgcccgtcg atcgttattc ccgttgctat cttccgcaac gggtcatttg 36540

acgtaacgga gtaaacactc cggtggccag aaaattctcc atttccattt ggtactttca 36600

tcgccgggga gtagctggaa gctgtacctt ttccgcgctc acttattaca ctgcatatag 36660

tgttgacccg tgttccgatg agaggaatct ccctttatgc gcaacgacca acaagtgaca 36720

aactgccttc gggtcacttg tgtgaataga acagatgcga aatgtacgtg gactttaact 36780

tatctaataa ggccaaatag caattaaaca ccctgaatat gtatggtcac gtagttttct 36840

tgcggcagat cgccgcgtag acgcttaaag atgcgatcca cgttatcgaa gttaaagtac 36900

gatagtttct cgccacatcc ttggtgaaat aaggcaacac atatgacgtg aaattcaatt 36960

acaaagacac gtcacttcga aaacaatggg cgtggttaca gaatacccta aaacaacttt 37020

tcaaatgcct aaactctttc atcttatact atagcagacg agcgcacatc ttttggctcc 37080

aatactgtcg ggtcaactac ataggtagtt atggattctg gcgaccagct atcagataat 37140

gaatattacg acctcgatga ggacaataca tgtagcgaca atagatcgcc tagacctgta 37200

ggtagatggc ttcttaaaga tatgatagta gcactaaagg aaataattaa tacacaatca 37260

acgccaaggt ggaccgaagt tgaagcgtca aaagtaaagg ccatcgtgag tactttttgt 37320

ctttcgcaag aacaaatgac tataccgcaa atttcgcatt catggaaaga ggcattcgac 37380

ctgctgctcg tggccttctc caatacacaa acaccagaag ttgctattat tatagagaat 37440

tttacaggtt tggtaatatg gcgactcgtg gtatcgtggg acagaaatac cgtcaaagcg 37500

gacgttacaa aacttatggc acttgtacga gatctgacat ccgaacacgt cacacagagt 37560

ctgacacgac aaaatttaag attatcaact agctatggcg tgtctgcgat gcgcggcaaa 37620

cttttatcct ggttaacaac cttcgaggct gcagttacaa cagttttggc aacaacgcct 37680

gatgttttac tcgactcaga aacgtctatg gattccgaaa agatcgtgaa ccctttacta 37740

gccgatacat acgcattaat atatgatttt ccgttcgtac aagaagggtt acgctttcta 37800

cataggaatg caaattggat gattccgttt aaaataatga cccgctgtgc ttcggatacc 37860

atatacagtc cattagtgcg cacaatttat actatatcat tagtggatca gtatttttgg 37920

ggggccgggc gttctaggcc taagaggctg gtcgatcaat tcgtaaaaga tacggatcta 37980

ttaggagatg ctgaattaat gtctccgggt gaggcaaact caacaaaacg aacatcatgg 38040

gaagttcgtc tatctgctgc tttagcttat caggacccat ttgtacgaga agtacaacca 38100

ggaatggcgt ctgtgcgcgt tcgtacatca cccgatatgg tattgcgcgg aggtccagta 38160

tttgggccag cgctctgcat acactcggca gctgttttaa atgtcatttc tgggtctaag 38220

caagatgagt ttgatttagg cagactgaat caagctgcaa aaactacaat tactgaagca 38280

gcaagggcag catgggatac aatacaacat agcaatacgc ctcaacaagt aattgatgca 38340

ctaatttcta cgggttttgt agcacaaaat tgccgtaatt atgaggtggc attaacctca 38400

atgtactcgc gagctactac cgataatgga tatgcgctaa atgataccca acaggtgata 38460

ggttgtgtct ctatggtcgg aaatgttgtg tttggtctca tagatagcta cgggcgcgat 38520

gccgactata ttgatgcata tgcagaggcg atgtcatcgc tcgagtctga cagcggcgat 38580

tttctttcgg ccattggtct accgaaaggt ggaattgaac aaacgatccg ccattgtatg 38640

gcaccgcggc caataacaga ctatatacgt gccgcacgcc aagcgctagt acaagaaata 38700

gaaactgcgt ctagtatata taaaggacgg ctttcttcga gactgcaaac acatcatact 38760

tcgacccaca acagcgtccg tggttcgtta ttgttgtggt ttgattttag agccaagcaa 38820

atttggggca tcgagccacc ggggaacgat gaacaatgta ctcctgaagg tcatgcggtg 38880

ccgagaagac gtcagacagc tgagaatgta acacctgttg tagcctctag aaatgcgtcc 38940

gaactaatga atgtagctgc aactatacaa ttccctagcg tcgctcccca agggactata 39000

atcgactcgg aagcattcgc accttatgtt gtagcaaccg ctggggcaga ggcgctctct 39060

acattagcat cggccctatt ttctgtatac catctagaga ctgcgatacg tgtattacta 39120

tgggcacgag aatatggtat agctacaata ccaaattatg acgggtttcg gaccaaaatt 39180

acagcattat tggcggcaat tgtgcccttt acgagccgtg accctccaag gccaagaacg 39240

cgcgatgctg agaatataga acgactgtta aacgaattgc agtcgtcagt aagtgctgct 39300

atagaagccc ttccagatga ttcaaggtta acaataccac ctaaaccaga tatatcaaac 39360

agtgcgcttc ttattagtat gcatgctaca gcattgcgta tttcagtaga tgatcttcga 39420

gagcgtacta tacatttaat agacgattta ctcatagcca tagactctct agcatctact 39480

acatcggctc tgcgactatt tttactttgc agattacgat caaatggcaa tggactaatt 39540

gttactatta gtgggcgggc tggcaatcgg acgacaaaat ttggttcatg gcgcctatct 39600

gatatagttt ctgctataga caccgcgtat ggatcatgcg ttgaactatt agcagatatt 39660

cgtatgaagt cgatggcgtt acgtaatgtt ttgtcggata ctaatgccaa gattcctatt 39720

tgtgatgcac tagccgcacg tattggcaat aactcttata aacgtatatt tagcacaatt 39780

gcatcagatc tggataagct atcaaatgtt caaacggcac ttgatctgcg gatacgaaaa 39840

ataacgtcag gttccgagcc cccaagtttg cgtccagttg ctcagtttct gagacgttgg 39900

acggagattt ataatgctta tcggtcggct cttgttaaaa aaacggatga aacatttact 39960

acattggcag aaacgctacg tattatatgg gacttgatac agaacgatcg tgccggggtg 40020

ccaaagggat gttcatatcc aacggacgat gacttggaac atgcggtact cggtttaatg 40080

acagaataca cacaaattaa tgatctagat gaaccatcga cacctctaac ggacaggcgc 40140

aatgtatctg acgaagaaaa tataaactat gcgccattgg aaatgcatac gcataaacca 40200

ccggaagttg atccggataa tcatttagat tactttttaa attctaagag gctgacacag 40260

gatgatttat tgtctatagt agacaaaatc ttcaacgctg aacatgtata actttacacg 40320

gccctcgaag tgtttacccc ctcgcttttg tttattaaaa caacaatatc cgtttatagc 40380

accatactgt tgatttacaa tcctaataaa agtgttattt gtttggggta tgatgaagac 40440

ttttccatta tacagtgcga cggtcgtcga tattactaga actgctcgga catgatatta 40500

gtgtaaaagg cgaaagtgct cagactgtta ccaaactcat tctaacggag acccttatag 40560

gggtttatag gccgtcgcct aacaaatgca tcttggtcgt taacggggat ataaaggggc 40620

gtgagcaacg tatatgcacg ttcaactgcc ccgtcggaca gatgcaacat aggatagcca 40680

gagagataga gctcgctaat taatcgtatc ggagaggtga ctaactacaa tggccgaaca 40740

gacgtctact aacgaacact cttcacactt ttgcctattg gggattggta cagaacaggt 40800

gatggcgata ttaggcgccg aacgcagttc tggcacgtgc acaaaagata tggaatatga 40860

tctcctggca gccgtctcgg aagagtatag gggtaataat tggagtagtc aattccatgt 40920

ttggaacacg ttatcggcag tttcgttggt gcattctatt ccggcggata ttaggtttga 40980

tggaagtgtg ggttccgaaa agccgtccgc gtcgccgcca tccctgacag ccgaccaaga 41040

tagcacgtct gggtctggcg gctccaggga cgacccggta ggtttcatca tcgccacggg 41100

tacaacgaac caatcttcat acgacctcgc cccttggggc gagaaggccg gcgcgctaca 41160

taccttggcg tttatgcgcg cgtcgttcct gtcaggagtc tgtgattact catctgatgt 41220

aattaatgca attctacgcc aaggtgctag ctttgctgaa cagttgaaca agcagcccaa 41280

aacatttgta catccaaatg aacttccgta tatcgttcgc ggggcagatg gagatgcact 41340

attttgtttt gtatccggaa cattcataat cacgactaca aaaacgccgc caaatcttga 41400

ggaaagatta ccatatgtac cagaaacccg tctgatcgaa gggattccgt ctatactaac 41460

aatttggtcc cgatgccacc agaaggcaga tggcttatat gtaaccgtcg gtaacacagt 41520

gttgggaatg tggcgcgatc cttccaaaga atgggtaaca gtttttacat acagtggatt 41580

gaaaaccacg acagatccag cgtctatatt tcgtgttcgt ctcaaacacc tagagggtat 41640

gttaatgtgt tataccattg gtatgcatat gctggccgtg aattttgcat attttctcag 41700

atctggcgat tcttctttgt ctgtgtatga aggggctacg gctgcggcca atttttatgg 41760

tacgactgat tttactctat gtaggaactt tagtggatca caagaacaaa ttacagtgtt 41820

agtacaagcc gaagctgtct ctgtagctgt cgcatctgta aggtctgggc caacagcaag 41880

ggatctgttg aacgtcatct ccgcagccga cacagtagcg caaactaaca gggatcaacc 41940

aattgtaata gtaagcaaat ccgtgtcagc ccacgccacc gttacggccg tgggttatag 42000

aaaccaatat gcagcagaat tgtgcccggg ttcatcggtc tcgtgtttgc gctcgtcgct 42060

ggcatttctt agagccctat atatttttgg aatcgaatcg tcatattctg ccgacttggt 42120

tgacgtattt ttgcgacaag gtcgagagtg gaccattgat actaccgcgc ggggcgaaca 42180

gccaagtatg tgctcactaa atgaacttcc gggccgcata gaatcatcag cgccaggtat 42240

tgtggattta tgtgttgtca cgtcacgtat atatggtgat tacccgtttt atggtggacc 42300

tggaatagaa aaggtaatgg aaacaactat ttccgcgcgc gtatttatag acacggtcat 42360

gacacaacgc aaagagacct actgtttact tataattgga gcacagggtt ttggattgta 42420

tcgtaaagat gactacgtgt ttatattcga tccccatggt catgctgata tatctcaggc 42480

ttttacggcc aggctatcaa tcggtaacct atatccattt ttaactacat acgctgatat 42540

ttccgccaat ccatattggt ccggcgtgtt agtgtacttt attgttaccg gtccggggac 42600

acctactact gatgagttga catgtgccgt ttccagacta tatggaactg gggatacaca 42660

atttttagag agcagcaagt attctaccca aaaagttgtg gcacgactgg acggtcttat 42720

tggactgaac gcaaaaacaa cacatactat aggcgtcggt ggatatgcac acgccccatg 42780

cgttacattt gaaactagtc aggggtggcc aatagatata ggtaataatg tatatgaatg 42840

cggcgacgcc ggtaagttaa aaaataatgc aaaggaagat aatgccaaat ctgaacatga 42900

ccacgaatct atgttaagtg atagtgtggc agtaaatgat ctttcatggg aagaacaaag 42960

atgtccgtgg aaaactgcct tcgaggaaac ggaaaattgt tcccaatcta cggaggaccg 43020

tgtaaacgaa ttaaatgaga tatcaacatt gtctccagtt gaagaggcta tttcaacaag 43080

cgatatgata tataatgcta ctggccaaat tacttcaccg gacttcgaac cagtaccacc 43140

accatcggca cgcattcgtt cgaagcgcag aaggcctgta tggacaccgc catcgagtac 43200

agagaacctt acaggcggat tggcacaaga ccgccattca cgtgttaaat atacaaagcg 43260

cggaaggcat aagagacggt ctcaagatac ctctagcagt gcgagcttat cgggcagttg 43320

tacagaactg agcgatatga tagcggatat tgcatatgca cccggtccta ttgccatgtc 43380

ttgcgagtcg caaaacaatt caattcattg tcatcagtcg ggcaatattg caccagataa 43440

tataacagat gaaagtaggc aagatgaaaa ctattctgtt gagatgtggt ggaatgatga 43500

atatttgaat gcaattgatg tgacagatat gacagataaa ataatggaaa ttgatacgat 43560

aatagaatcg gatatgtcat cgccagtaag ggtagtccgg ccattaggaa gcaataacga 43620

agatgattta ttggaacaat acatcctcac aatttattcg cgcatgtatg cctttattat 43680

agaaaatggt gcacggactg ttccagaacg tcaatcatta attgcgccgc tgatacctgt 43740

catctcggca tcgttcccac gaaaaactgc ctttggggct tttatagaat ccacaggtat 43800

gatactcctc aacgtcccga tacacgccga acttatatca tcagtcagaa cagaaggtgc 43860

tcatctagga ggactggcat taattaaaat ggtattaatg gcgatagatt cagctgtcgc 43920

ttctgatgaa ctacatacac gtttagatgt tttgagagaa aatgctcaga agactggcac 43980

cggtggctat gaactagtgg cgtcggcatt atcagaagaa tttacaaaaa acacaattgg 44040

tatttatgcc gagaagacat acacacatcc ttcagtttca ctgtcggatc gtttaaaagc 44100

tatatgcgaa agcattaggg ttaatgaact ggaagcaaag aaaaccggtt tagagttagc 44160

aaaaattcta gctgctatcg gaaccggcgt caggcgaatg catgattatt atgacacatt 44220

ttatatcgac gaagatcccc tatcggctcc catgataagc gaaacattat ctccaattag 44280

agctgatgat gtacccgcaa agatctccga gatttatgag gaggccgtag gtgtactcgg 44340

aaatgctata cgggaatact gtctcaacgg agcatcatac agcgccaagg cactatcggc 44400

cgaccgcaat ggtcgtacgc gatttaaaat agccagcgtt gcaattgatc ctattacgcg 44460

tatggtgaac tctctaccag catttgatag cgcattaaca atgctggctt cgcgcgccgg 44520

agtggattct ccgcaatcaa tatcatccag tagagaagcg ggactactat cagatctatt 44580

aaatgcagga caagatattt ccaatgacga aacactagga aattggttag ctctcatgac 44640

tgaagcgcag atcaatggtt atatagaatg taaagagctt gccgcattaa tacgtgaaat 44700

aaataccata aacgaacgag ccgcgcgcca tgcatctata gaagcggaac ttcaaagatt 44760

cgaggcactt tcggcagctg ttcaagaagc gttgaatggt gcagctaggg gcggaccggc 44820

cgcagtgtca ttagacacgc ttatacgcgg cgcggaggaa ctagtcgccc gtgctcatta 44880

tattgaatct ccacgcattt catgcgattt gactgatgaa cagcgctcag ctgtaagaga 44940

tcgtcgcata gaggtagaat ctatgttaca cacatatcgc gggaaaatgg acaagattag 45000

cggtgtactt agtagcctgt atgaaagttt acgtatgata tttaaaccta ctaacaactt 45060

cattggtctt agaaacatca atcaaaatgt aaaagcctta acagactctc tgcccccaga 45120

actcggatcg ctatctgccg tagtggccaa tgcaccgcaa gatgtttcga gggcaatgcg 45180

tacagactta tggacacatt tttcgcatta ccgcgaggct ttagaaaaac caactacatt 45240

tttagctacc gaattgtcgg gcctgggtcc ttgttttaca ttggccatta aaataactac 45300

tgaacatatc ggcggtttcc ccggggcatc tgttttcttt ggaaaacatg cagacggcgt 45360

tgctgctgcc ataacggcgg cggtcgccag ccctaaatct gcaaccgcta caacagcagc 45420

cataaatgtt ctggaagttg ccatcgggga ggtggatcat gtggtaacaa aaatgaagga 45480

caacacagcc aagaatgcca tgtcatttct gagaccaata gtagacaaat taaaaacaca 45540

tcttatcgca atcgatgaac ttggtcatgt cgaagcagca tcccgtgcct tgcgctcggc 45600

atctgaaaca tctgccgcgt tagcttcaaa aatacgggcg tccgatccaa gcataatgga 45660

tgatacatca tttgcagcaa tggccgcaga agcaaagcgg gtttgtaacg aaacgaaggc 45720

agttgccgat agtgttgaac gtgacttggc aaagacgcca ataaccatcg ccgcgtcggt 45780

atttaatgtt aaagaggcag acatattaaa atatatacag gcagctaggc aacgagttgc 45840

ggaagttgat tcggcaatga gcgatgcctc aaagactcat cagacagtta attcggagcg 45900

ggcattggca atttggaaag actcagtaac ggcagctata gttagggccg agacacgctc 45960

ggaatttgat cagcaagagc tcacgcggtt aagcgacatt gctgcggtcg atggatacaa 46020

gacaacctgg attaaaaaac gcgccgaggc cgtagcagcc aatcatttat cattggtaac 46080

gacagccttg gatacggttc tacactttaa cccatatagc acagagaata tgcgcacaaa 46140

ggcaccgccg ccaatatctc tgctgcgcga tattacatgg ggagatgctt ttatggctgc 46200

cgcgccagct ttctccgaac tgttcgaagt tgacgtagag ttattgataa gtgcattacg 46260

gatgaccatg actgttttag cacatattag tgccagtgcc ggatcggtag atttttatga 46320

tttaattggg catatagaac gtgatttaac gcgccatgcg ccacttttaa aatatataca 46380

gttttacagg aaggggaata ctaagttcaa ggaaatagta ccaatattgg atgctatgag 46440

ggccgacgtt tgtcaagcta tgggccaaat accctcggag ctcaatcgcg ttctggagga 46500

catttcgcgt atacgctgcg taaacacagc aagaaatata ttagaaaaca gtgtacgttt 46560

agagataccg agtatggcta ttatagaacg gatagcatcg gaattagaaa atctagacac 46620

gcaaccgctt aaggataccg cttacattga atatgtagaa aacgcccgca aacactatct 46680

ttccgaagca aagtctgcaa tggatacagc ctttaatatg tatcgtgatg ctgaaacaaa 46740

ggcgaaaaca ttactcaata atataataga aaacgataag gccttggaaa aggataaaat 46800

agagggatta tctaacctta aaaacttact aagactaact ccactagctc ctcattatat 46860

gaaagctatc gataaggcag aaacggcaga tgatattgta acacaagctg cactaatatt 46920

ggcgcatgta gagaaaacag aagaactaga tgttcaggcc gtcgagtggt tacaacaagc 46980

taaggcaatc atagactctc atccccttac agtgttaatt gatgatagcg gtccaatggc 47040

accttatgcc gaccgtatag acgcgcttgt gaccttgaga agtcatctgg acgaactgac 47100

aaataatatt gccagttccg aagcggcatg ggatgccgcc tgggataatt atattcgaga 47160

taaaaacaaa gtcgatgtag atgcggacgg atatgctttg gcaatgaaca gggccaatac 47220

tctccaggcc gctggaagtg tagtattggc attgcgcgca gattcggagt atcgtcgcct 47280

tccgaccaaa ctaacctcgg cattagatac gaaatatacg gaacggctgg ccaatttaga 47340

cgcatttacc acacggactc gtgatataga tgatgcctct cggcatcttt cgtcgttgtt 47400

aacgaaggtt ccgaatacat atgatgtgcg atcattgcgc caccaaatgg cccagctagc 47460

ggaactactt tctaaattac ccaaatggtg tgccgaagac gcttctccat ttaagcacct 47520

actacaacta cgtttaactc tatacgctgc ctatgcagat ttgtgtgccg gaagggctgg 47580

cgtttccgat ccaatactcc atcaaagtcc cataagaaaa caaaagaaaa ttgacgccgg 47640

tgggggtata gatagactat tgaaatgtcg tgtggcggcg aggctcgaat cgcgtgttat 47700

tatgacagtt agagaatata catctgatat agatccaata cacataacat atatggatac 47760

caccgacgat cctatacact attccatgtg ttatagagca gcatgtgaca agattgccgt 47820

taacatgttt ggagatggga ctgccctact tcctcctatg ccacgtattt ctttatccga 47880

cggcgcttca atagaagctg cgagtatact gcgcgagtta ttggatatgc gtgttcagta 47940

ttacaaagcc attggctctg attattccac attcgcgaga tttgtacggc ataagagacc 48000

agattggcgt ccggaagagg ctaaccgtgc agtcgccgaa acttattcag cgttgatagc 48060

cacgacacta tgtagacatt tagggggcgt tagctgggga gagatatcat ttgtagaagg 48120

agcaactgga ccggtagcat gtccatgtac acttatacaa gaaccccctt acagtataat 48180

aggaaccaaa gatgcaaaac gtagcgcacc agtttctctt gatcttggag atgttatggt 48240

cactcttata gcatgggccc cgcaacatgt attaacgttt gtaacgctcg acctagttct 48300

tcagcatgaa tatatgtcaa agacgttacc tacagccgtt gcgactgcgt tctctagtag 48360

attgctcatc aattctttgg atctatcgtc cagtaatgat gaacacacgc gtgtactacc 48420

cggttgcgga aaccgatgtg acccttcaaa tggcagctta ttcgcgcttc gaatgattga 48480

ttggcgacgc ggacgcttat cagaagacga tccattaaag ccatgggacg aaaacagcag 48540

ttatgcaaat tcccaaacta tgataactga cattgcaaat ctacgaaatt ccataccgtc 48600

ccgtatattg acaaccatga ccgttttggg tcgcatgtgt attccaccgg cggcgctagc 48660

agcgatgtgg tcagtgttgc gtccagaaga tattgggtct gagatatcca cgtatgatat 48720

gttactgcgt gcccgttttg acacatcatc tatgattgga acgtcaatat ctatagaagg 48780

acattgcaat aatgtggatg gttcaacaac gaaacggacc aatgtttcag gaaaacctaa 48840

atctctgtat gagccaactg gtacaacagt ttcatttact gtaattaatg caccaccgag 48900

taatgttagt cgcgttaatg ccatggatat ggccgttaca gcaactctcc ttggcgctaa 48960

agtagttata gcggcagagt atccaggtgc ctactcggac ggctctggcc taaccctatg 49020

catgcgtttg tttgactctc gtgataattc agaaggttct tttataaata cttcttcagt 49080

gtcaacggac ctatcgtcat ggggtataaa attattggat cttgatccaa atagtataga 49140

aaactcctgt ctaatagcac aacttgaaaa cctatctggt atcatagcat caaaacccct 49200

agaacaagcc gacccgtgtc taattctggt cgatggccaa atgcgcccta cgcaggtatt 49260

atggcccaag gaggctgtta aaataccaat tatacgttta gtatctgaaa aggcagatgt 49320

gttggacgaa ctgccgtata tcgacacaga agaagaaact ctaccgcaga catcggacga 49380

tccactaatg gtcaatattt taggttcggc tgatgcgtcg ttttcaaaac ctgaacaacg 49440

ttatccaatg ccccgttatc tttcatctac acggcataac ccatacgata gtgaagatta 49500

tgactcatct ttatctgagg acgccgaaga tgcaaaaaca attgacgggc cgtctatctt 49560

cgaagactat aatgaagacg acctattcac ctgcgccgcc tacgcgaatg ataacaacga 49620

gcaggacgtc gcagatgaca tgtcgacccc atgggcgaaa tttctagtag atgatgcaac 49680

aaatgtcggt tgtgatgtta acagaagcgg ggctgctagt tcgcctccga gccgtattgg 49740

tgaacgcatt caagagcaaa ataatggaca aactggttct gatgagaaca gaaataatag 49800

cgtagctaat gcatgcgcaa ctgacgagaa gcacgcgcat agtcctgcgc gaccttctgc 49860

cggattgaag gaatgcacta gcattacaag tggacggtct accaatgaga aacagatgag 49920

acggaaggcg caccacgtac gcgatcggga attcaacggc agagatgtat ccgatgacaa 49980

tactatacaa cctgtctcac attctccaac agacagtaga ccggcagggc caatcgagcc 50040

gacagccgat atacctccaa cagacagtag accggcgggg ccaatcgagc cgacagccga 50100

tatacctcca acagacagta gaccggcagg gccaatcgag ccgacagccg atatacctcc 50160

gcaatccgcg gcagcatcga cggaatcgtt gacttctcgg gcaaatgcgc cgtcatcgca 50220

aacatgttgt gatactaatg ggccacgagg atctgtcgtg aatactaacg ctaaaaaagg 50280

tataccgcgt gatacaagcg tcaattcagc ggcctccgta aacgttaata agtctgttac 50340

gcctaaaggt cgcgaaagct acaataaggg cgttgggttt gggccatcgg taacgccaaa 50400

cgcggccgga gaacggcata cgcgcagcag tttgggcaaa caatcgcatg tacctgtcgc 50460

ctctgcagat aaagagccat cccatagtac gctgagtaca atacagaata gtgttgatga 50520

taggccgaaa cagttaaaac ccggttcccc caaaagtcag gaaacgtcta gacatgctgg 50580

acgtgaaaac actggaaact gtaataaatc cagcgcagat gttttttcaa catcagaatt 50640

cgtgtgcgac ggtggtgttg atgtgtcaaa actgcgttgc aaagttggaa aacaagagtt 50700

gtgccgccgt cgactcaccg acactctgac cgaaagacga gacggccaga atggtggtgt 50760

gtcaaaggcg acactcaagt cttgggcgtc aaaatctaac ttacctatgg cagcgtcaac 50820

atatctctat cgtccaaata ccacaattcc agcggatgtg ccaagtgcgg cgcatgacac 50880

aagacataag gcagacctaa catgcggcaa tggattgccg acagcacctc agtataaaat 50940

tgtaacgagt aataagcaac cagggggcgt cagtgtccaa ccgcccgata cttcccctgc 51000

tcagatacat caatccgata cgtcgcatac caaactagta accgatggtg ggcataaaag 51060

cggaggactc gaagcaggtg ttttaacgca gagccgtaaa atgcccaaat ctggtatata 51120

taaagcaccg ccaccgtcgg tgcataggaa caaaactgaa actactccca aatatgttaa 51180

aacggccaga tattcttcct ttgatccatc tttagaccaa gaccattcat ctgcatcgga 51240

atcggattac gatgagaacg atgaagagat aagtttgtcg agctatgaaa gcgacatgga 51300

ttcgccggaa caatcacata ctgatacaac acaattaact cctagtaaaa acggaagggt 51360

aattcccacg gaccacttgc tcacgcgccg agaattccgg cgggcaggga gaagtgtcct 51420

atattcactt ctacaagcat gtagacagat aacacgttct ctatctgacg ttcgcacatc 51480

aataatagct agaagtagag aaataacgct cgaaatactt aaattaaaat taatattagg 51540

ctgacgcaaa tataacatac tcaataaaag cttttatgaa tctttattga atcatactca 51600

tttattttat cgctgatcgt ctggcgcgtc gcgtatgatg cgtggagaaa aggtgcgctt 51660

gaggccaaaa ctcggtcgta cccacgatgc tccatctgtt ctgccaaaca tggggtatct 51720

gctgagagta gcgtcatcat tcgctctgcg tatggtagta agtccagccg acaacgccct 51780

gattaaagcc aacctcgccg ccgaaactgt gttttgccta acattagcat gcccacttac 51840

tgaatttagt tccattagca acgcaaagaa cggtactgcc gatagtgcct catatgtgta 51900

ggtctctggc cttctaggat caaatgtagc ctgacaagat gaacctccag aattagacat 51960

tgttgttatg ccgtagacgt ggtgttgtat gaagagcgtt ctctatactt aaatcgtccc 52020

tatgtagagc ctacgccaga aatgaaaata ggaactagat acggccacca aaaggccaaa 52080

tgactaacgc aaacatgaca atcagtatac atagtgttag caccgcccac atagaaaatg 52140

acgtattacc ccgcaataga ctaatgatct tccagtttcg tagagaatgg aacaaaccat 52200

ttttttcgtt actgtttggg tccaaatctt ctaaaggagt cttcttaaga tccggttcgg 52260

tgcgaggttt aggtatgcgt ccagtagcat cagagtactg tttttttgct tccatcattt 52320

ctgacatcct tagaatacgc gcggctcttg atggatcttc ggggcccata aatctgaacg 52380

ataactgtac acaaggcata cgtacaaaac acactaacca catgcatgat tgaagcgggc 52440

gcgcgtcgat tccagccatt gttttcattt tctctatagc agatagactt atgccggccg 52500

attctgtaga cttcaatatt atattattac gatctacagt aatcgccgta cttctgcagt 52560

cctgcggtcg gttgaaatat ccttgtacaa gtacagacac ccccgtattc agtattttca 52620

caaatggatc acatggtatg tctctggccc agtctgccaa catgcgcatt acatactcca 52680

gagggaacgt accatcactg ccatcctgac cgttaaattg atagacacat cgcgttggga 52740

gtctgtcgcc acaagacgat aaaattgctt ccccatcgca aggttgtagg cccccagcag 52800

caaccaactt aattcgctgt attaggtccc taccaccata accgcgtctt ctgaaaccgt 52860

ctggacatgg gtaaagctgt tccatagccg ccgtaatata ctattgtata tggtatcact 52920

tatcgtccaa cgtattagta cacgataatt gatcaagtaa cacattgccg ccttttatag 52980

cattccacta tgacgtgtca tacttccacg atcgaactga caaatcctaa ccaaagcacc 53040

tcccattatg aatatgccgc cgataagcaa catacattta tatggcagca taactaacta 53100

tcgcgcaata tctgatgtag atcgagcaag cgatttatta ccgctgcaaa tctctccctt 53160

ttgtcatgaa gtgccttatc atggcagcat gataccgctt tggcgtcttc tgcatgctca 53220

ttgtcaactt cttttcccat tttgttgtag ccatccacac gtatgtgtgc atgcgccaaa 53280

actgatgcaa gcctcctagt aaaggacaaa taattgagct tacagtcagt agtgggaaat 53340

atagcctcaa actccacagg tacgagctct tcaaaccaaa tatcgtaagc aaaatcatca 53400

ccgataatat acttgtcaac tagctcagac agcgttaaac catctaggtc ttcattgggt 53460

atggcatcgc ggagtctaca cgtcgattct acacggtcca tggcaagatt tgcagaagag 53520

gtaccgtcga cagatggcga gggttggaca agtggtgcgc ttgacgctga ctactgtgcg 53580

tttgatcctt cactacttgc cactaatgag cgtttgtgtg atgaattaat gtttgctgcg 53640

catcttatgt cagtccctaa tgaaaccgct actgaggtac gtgatgaaaa cagggatgac 53700

attaaccctg cgagtgataa tctattggtt cccgatttgg cggcgtttgt cgaggcttcc 53760

gctgatgcat ttgcattaga tcgttcatgc ttagtgtgta ggttaataga tacgtataag 53820

aaaaaattcg gtctaacgtc ccaatggatc gctgattatg caatgttatg caacaagtgc 53880

ttagctgcgc ccccttgcgc gacaactaca tttatcgcag cgtttgaatt agtgtacatc 53940

atggataaac atttccttgc gacgcatcgt accacactta ctggatcgtt tgcacgacgc 54000

gtactcacat taaatgatgt tcagaggcat ttttttcttc atggttgttg tagaacagat 54060

ggtggtatac ccggttctac atcttcaatg aatcaaccgt tagaatctac aaatacgcgg 54120

attacttcgc caatggtaca ggcaagaaca tctaggtcta ccaaagtgtt attcagcaac 54180

tactcctttt tggcccagac agccacccgt acgatgttaa tgacattggc cgacaattct 54240

tcaaacgacg ttcagaacaa tggtacgcaa cgtacaaatt actatagttc tggtcatcat 54300

agcacgctaa cagctttgcc aggccagcca tcgttagctg cggcattggc aggttggaag 54360

gaatgtgcta agcatgtaga gtgccacggt tctgctgctt ctaggagtgg tgtcggtcaa 54420

aaagatagtt gcgctacctt ggcgtatgtt gacgatgacg catttgaaat gcatgaagaa 54480

ctgcgcttgg aaagggaacg cgagcgagca aaatctcact ctatgtatgc acgtcctaaa 54540

agattggcga ctaggtcaaa acctgcgcga gacgaaaatg gggcaacacg tgtacatgga 54600

accggaccat cggaacaagc ccaccgagag gactgggcgt atgcagattt gacattgctt 54660

ctactagtcg gaacaggggc tatttgggaa ttaaacgaca cgaccaatac cgtattactc 54720

gcaagacgta atgcggtggc gcgttactgg cgagaccata aacgtgcact ggcgcgagaa 54780

accgccccca aattctctag atttgcagaa aacgatgcag agccagtgac ttctctcggg 54840

ccagtactcg caactacact aaaacataca agatgcaaat ctcgtactgg cggggaatgt 54900

atattatgca acctattacc aatacgtacg tattggttgg cgattcgccg ccttaaacgt 54960

gatatagttg cacattccgc gaacaactta ggtctatttg attgcatagc accagttata 55020

gacacttggt ctgaaggcga atacgaagaa atcgccgacg gcggtaggtt tgtagccatg 55080

gtaaaagctg ccggcgcaga agcagtttat aaacatcttt tttgcgatcc aatgtgcgct 55140

ctttgcgaat tgcagaccaa tccgcgtgta ttatttgacc atccaaccaa atcggatcca 55200

gatgctctga acctatataa ggcgcatttg gcgagcgaaa accgttttga ggggcggata 55260

tgcgcaggtt tatgggccct ggcttatact tttaagactt atcagatatt cccgcccaag 55320

cccaccgcat tggcctcgtt tataagggat gctggtctcc tattaaggaa gcattcaatt 55380

tcgctcgtct cattagagca cacactcggc acctatgtct gactacgata ccacaactat 55440

gcactcgtct gctaggcgca gacgttctag ttcgttcgga cccatgagag ccagatcttc 55500

tggatcgaac gtaccgtact ggcgaccgta tagcaggaga cgatcgatgc ttcgcccgtc 55560

aagtcagcgc ggcgaaacgg ttgaacgata cagacggttt tttgaaatga tttctagatc 55620

cccttcagat gagttagcca tagtacggga gctaaacgta ccgcttatta aatcaactac 55680

tgttgtactg ccgtttgacc tggacatggc cgtcgcagac aattgcctat ctctatctgg 55740

catggggtat caactcggta tcgggggttg ctgcccagta tgttcatcgt cgggggaacc 55800

tcgtttgaat cgcatagatc gctcaacttt gatccttgca tatgtgcaac aggtaaacca 55860

cctataccag taccgtacat tcctcgcatc gatacaagca gcatccgacc gcatttctga 55920

tgatgcatca gaaacggaaa atatactggc ttgcattagt caacagccag aattcttttt 55980

cgcgtattac gttttacgtg aggctggtat gcgtgacata cgtgtattat tttaccgcga 56040

tgtagacgaa ggcgggtatg taatgtacat tccatttact ggaaatgccg tacatctaca 56100

ctatcgtctc atcgactgcc ttaaatctgc ttgccgggga taccgtctaa tggctcatgt 56160

ttggcattct acattcgtac ttgtcgtgag gcgcgaccgc gaacggcaaa ctgacgtgga 56220

cagcgtacca cagataagta ttgaagatat ttattgtaaa atgtgcgacc ttaatttcga 56280

tggggaactt ctgctagaat atcgaaagct ctacgcagct tttgacgatt ttcctcctcc 56340

tcgctgagtg gcatccctgg gtacaagcgc acttctgcaa acccggccga agatagcagt 56400

gctgcggttt cgtcactctc acagtatgtt tctggaataa agcgttttaa aacagcttcc 56460

gaagttttgt gatcattacc gaatagagcc ttgaaagtta cacttagagt ccccaacaga 56520

tgcgaaaagt agtagtcggt gttaagcggt atatgattag ctaaaacata gcccgggtcc 56580

tctgccaact cagaaaccaa tagcttgcgc ctcttcgccg caggcccatc agctttgcca 56640

gtggaattac ttggccgttt gtcgccgcgc atagtagcaa cacgttcggc atcgcgttct 56700

acgcttggga tttgtgccac aattacataa ggaattcgtt cttttatcgc aggaggctgc 56760

tcttcgcgca tacataattt gtagtagacg gtcagatgcg gtatgcgctt gttgacatat 56820

gcttctggcg cgcggcttag ctcggcagtc atgacaaaat ctggtacatt tagttcacgt 56880

gcacgaattt tactatatgc ttcagctaga gtagcgccaa attcacccaa accagggggc 56940

aaggaacgct ctagccatgt tttggctggt tcctttgaga tcgctgccgc cgcggcagct 57000

acttcatcat taccaaaaag aagatcgacg agtcgtttgg cataataatt aatgaattta 57060

cagttgtttt ttctgactaa gtcaactcct ttcatcatca tctttccgcc gacaatagtc 57120

ccaatgtatt tcttcttagt gatcaatagc aatctgaaaa atgttttctc acattctagc 57180

tttatcggac tggtgaataa ctctcgggta atcatactgg ccatagcatc accaacggcc 57240

actaaacctt ccggcgcgat tcctttaatg cgaacaaaca cggaatcggt atctccgtaa 57300

atgaccatgg cagaatatgc ctgtttgtct ttaacatgat ctaccaattc tgggaaagcc 57360

tgaactagca tatcacaagt agtccagtgg gtatggatgt agtcgcgtac tgatagtaac 57420

atatttcttc ctactgtcgt aaccgtcgcg gcgacgtgta aacatggcag taagccatta 57480

ctcactccac agaacccata cactgaatta cacacgacct taatagccag ttgctgctta 57540

tctaataaca aggcctgttc ttctgtgcta gttggaatct tggctcgtat tgcttttctc 57600

atagcaagcc agtcgcgcag taaaacgctc aacaggctta cgcgtatgtg gggcttaaca 57660

aaaaataaac gctgccctgc aacagtgata tctagataat catcagcatt taaatgggaa 57720

actgcttcct cctttagggt caacgtagta aagcacagat tatgagcttg gattatgctt 57780

ggatataagc tggcgaaatc gaacacggtt acgggatcaa catgaaagcc agtacacgga 57840

tctagtactt tggcgccacg ataaccgaca ctgcggccac ccttcatggc tgacccagat 57900

gtcattttag tattgtcagt ttcatctttc tcttcatctt catcgctgtc ttctagatcc 57960

tctgcttgta gcgctggagc taaatgttca ctgtctccac ctttgcctga aaaacgtgac 58020

ttattttcag gcaaaacgaa tccatgctga ccagctaatc tcaataagca agtgtatacg 58080

cgtacctgtt gaccatcaaa tactacacgc ggaagaagga ttccagcaag ctttgcgacc 58140

gccgacaatt caagatgggg aaggtattta aagaatagtt taccgacgag cactgagtcc 58200

tgaatgcagt attcacctat aagtccacgt ttagaaggtc ccgaagcata ataaacgggg 58260

atatccttat atgacaggtc tcgtttatgt tcgcccaatg cagcagaagc tacagtttcc 58320

aatttgtagc ttgatagttt aagcttttcg gtggctaccg aatacatatc cagggaaatt 58380

ataccgttta tcttaacctt actgcgtttc tggaaatggc cgccgccaag ctcccaaaca 58440

cgaaacatgc ctcctttgtt catcgccccg tatccatcga gcttaattcc ataaacagtg 58500

gtcaatttgt tatataaaaa cgcccaatcg aaattaacga tattgtaacc ggacacgaat 58560

tctggagagt attgctttag gaatgttaaa aaacatagta atagctcata ttcactgtca 58620

aactctagca cttcgggttt tattgccgct ccatctttgc cgttcaattc aatttcaggc 58680

aaatcgcacg accccaaaga aaataacaat gcatgttcca tgcacctagt ggatagtgaa 58740

tatagtatac acgatatctg aatgaccaga tcatcttcat ttgatgccac agggaaggct 58800

aattcgttct gcccaccaga tttacattct atatcaaaac atagtaactt gtagtctggt 58860

attgctccat cctcagagtc accaaccaag ttatccactg tgcaattgat ctcaacatca 58920

catgaagttg tgtggtgttc tggagcacgt acttgtatgc gctctccatt gtttccaatc 58980

ttcaaacgat accatccaaa cgtgctaaat cctatattgt ctaatatgag ccgcgtcgtt 59040

gcgtctacat tactttcata tttagtgacg tttgggagaa agttttcact taaaaagctt 59100

acatgcctac tacttcgggc cttgatgcga tagaattcgc atggtgtcgt gtcaaagtag 59160

tagatatcac agcgttcaac tatatctatt tcgaagcact ccggagaggc gttacgataa 59220

ccaaaattgc gcccaccctt tccagctgaa gaaaattcgc ccgagtggcc atcagaacct 59280

ccacaacttg atgaacgtaa tgccgttgcc atatgttcgg caagatcgcg cgggcatcga 59340

cttccgcatg cagcatccgt ttcagatttt ttcatgtaga agtattgaca tataccatag 59400

acatgaactg ccactttctt accacattcg cttaagccaa gcattgttac tattgttccc 59460

attgggctta gcaatttaac gaacctttcc gcatcacgcc cggaagacga ggatatagtt 59520

tccacatact ctaccatatc gtaaacatga aaccgtgcaa accgcgggtt aaagtcattg 59580

gcaaggaatg atctttcgcc ccatacatga gtacgttggg tccataattc catacattgg 59640

tcgtagttaa gaaattcgaa tgtcgcgcca ttgcaatacg ctataggttt cctacgcaag 59700

acacctatgt tggtaccttt gcgtttagag tcttcggcct cctcgtcgag tgatcgtggg 59760

gcaatgaact tgaattccga aatgtctgta taatagctgt atctattccc acgtaccggt 59820

cctttgcgat tgccgcgacc tgctgaccca tccaagtatg gattgaagaa gtttctaccc 59880

gactctgcca ttatattttc aaagtttaat ccacattggt gagcgcagaa attacttcgg 59940

tgttattgaa ttatgcgaga catgcgcaga atgtatttac gtcgcgaagt aattgtttta 60000

gagcaaaaga taaaaattat tctttgggcg gtgtcaaatc gtagtgtatg gccgtcgccc 60060

gtttgttttt atagcaacgg catagacatt ctattggcca catttacata aatgtaatta 60120

agtaatttgc atattaagcc ctgcctaata tttaacacat aaaagtacat gtcttctttc 60180

tctgtacatt gcctgcggca gctatcattc gaaagagtgg taatctactg acgaggtatt 60240

agtgtaccgc ggattattca agaatggaag gcgctgggaa gactatacaa ttaaaacctg 60300

gacctatagg ttatatatat gccagggacg tcaaatcgtt aaatgtggac attatatcaa 60360

tgctatcagc tcgcagcgcc gattcagatg ttgcaattct accattaata cgcggcctga 60420

cagtggaacc tgcgttcgtc tccaatgttg cagctgtggg aggctctagg acaacaggcc 60480

tcggcgggaa tggaataacc atgaaacttg taccgacgca ttatttccct aatgtatttg 60540

tgtttcatgg aggaaactgc atctctccta gtagtgccgc gccaaatttg accttagcat 60600

gcgaaagtgc cagagagcgc tttggtttca catcgtatac cgaacaagcg gatgcgacta 60660

gagagactac aggcaatgat atatgtacaa cgattggagt tgacccagaa aaaactataa 60720

tttacttggt cataacggct atgtttaagg agttggtgta tatgtgcaat acatacttgc 60780

attatggagg aattgagcgc attgatattg acaacacttc agtcatcaag attccaatct 60840

atcctataca actggtgatg cccgatgtca atcgcttggc ccccgaacca tttaatagca 60900

aacacagatc aataggtgaa ggattcgaac tgccgcgcgc tttctacaat acgaaactta 60960

atcgtctatt acatggggcc gttttaggac cactcgcggt ggccacgcgt gtacgcaaca 61020

tcgaatcagt tgcacgggga gctgctcatt tatctatgga tgaagcgcat gacggttccg 61080

tgctgcccgc cgatataaca tttacagcat atgatcaaat tgtggacaaa agctataata 61140

agtctggtcg cggtcaacga gcccaacaaa gtgcatcaaa ggagaatcaa aataacgatg 61200

gacatgaacg ccgcctcgca tcggtcatgt ctgccgattt agcattaacg gtagatacac 61260

tgctttctgc ggcaatacat gaagatggag caactagcat tggcgagtgg ccaattatgt 61320

ccatagagga tacaaagctt aaacaagaag cccttggcgc atacatggcc agatgcgccg 61380

ctcttgtttg tgcaatggtc tttagtacca actctgcatt gtatatgtca gaagtttatg 61440

acgccgggtc tccagatgcg ggcaaagaca gcggacaacc atcgttttat aggttctatc 61500

agatagcagc tccatacctc gcatcgaacc cacaaacaga cagagatgga cgcatcatcc 61560

cagaaactcg cgatcgccaa gttaactctg tcaatggaac cggtcaagac tttggaattg 61620

atcacttagc tatggcatgt ggcttcagtc cacaattgct ggcacgtatt ttatattacc 61680

tagagcgtgc cgacagtggc caacttgtcc acaaacagga gctagatata attaaatatg 61740

taaccggttc aatgacagct gatatctctt gtcaattatg caatcgcgat acgagagcag 61800

tgtgctctca taccacgctg catcgtcttc gtcacagaat gccacgtttc ggtatgccaa 61860

tacgaggtcc tataggtgtt tttggtgtag taacgagcgc gtatagtgat gttgaccctc 61920

taggcaactt tgcgccatat tccgcactta aaaaaggcga tggagaaatg gcacgcgcag 61980

ttatgcagga tacgtatcgc gcaacagcag agaaaatcat gactgaattg gaaagtatgg 62040

gcctatttga taaggaaaat cctctagcat ctgccggttt agaagcaata gtaacagacc 62100

agcaatcatt ccaggcaaca ttcacaaaca tacgtcaact tattgaacgg gaagccgaac 62160

agttaatgag gaatctaatc gatgtacgtg actacaaaat ccgcgaaggg ttaactgaat 62220

cttcccatac attatcgctt tctgttgatc cgtatgctat tggaatatgc cccgttcttt 62280

cattcctcag caaacgatcc atgctagcag ttgttcagga ccttgccctc agccaatgcg 62340

cttatgtgtt caatggacaa caagttgatg caaaaaactt cagaaatcaa tttcagccag 62400

tactcagacg ccgtttcttt gacttaatga acggcgggtt tgtttcaacg agacaatcga 62460

cggtaacgct cgccgattcg accttaaccg ctcctgatcc tactaaatcg cagactgata 62520

ctaattgtgg cgaatttgat ggtgagcttg tgcgcgtaac cacagaagta attcgcgaaa 62580

tgaagatcaa aaatcgtgtc ttgttttttg gttctacttc caacatgtct gaagctgcaa 62640

aatctagagt agccgggctt tcagaagctt atcagcgccc ggataggcgc gtagatgttc 62700

tcggtggagc acttggcttt gctctcaaac agttacacgg tgtactattt ccgaaaggaa 62760

tgccaattgg agatgttagc cctaatgccc agtggttctg gacaatgctt cagcgtaatc 62820

aaatgccagg caaattatta gggccgaagg atactgaatt aatacgattt gttaaacggt 62880

ttagtgatga gtacgctgca atgaattata taaacgtcgc ctcaacatgt gtcggcgatt 62940

tggcccagtt catgctatca aatatcattt tgaaatattg cgaccaccgc aattattaca 63000

tcaattcgat aactgctata atggctacct gtagacgccc gcgcgaccca tcggccgtac 63060

ttcattggat agagcgccct ttggcggatg ggaatgatgt agaaaaggcc gcggctgagg 63120

tattggaaac tgtggcaact cgcccccatg tgtggacaac ggcctattct tcaaataatc 63180

tagtacgcgc ggctctaacg tccagaccgt ttgttgtact agggatcagt attagcaaat 63240

accaaggaat ggccggcagt tcacgggtct ttcaagcagc taactggagt aatttgacag 63300

gcggtaaggg cgtgtgtccg ctaatgaatt ttgaccgaac aagacgcttc atgttggcat 63360

gtccccgtgt agcgtacgta actgttcaac aaggcggttt caatgccaac gcgagggagc 63420

gcacgctcgc agagcaagtg agagccatta tgaaagaagg tggcgtgact ccccaagcta 63480

ccatttttat agcagcttta aaggctttgg ggcaacgcgt acaatgtatg gataggcagg 63540

attggctttc cctaaccgag gatgaatacc tatctgagct gttagattcg atgaatacca 63600

gaactgcaga acgcgaaggc ggatggagcg tagatgctgg gatggaattg gcaaaagaga 63660

ttgaggcgac atcatctacc aatattgcag acgaaggaga tgctgtgttc gattttggcg 63720

cttgtgatgg cgataatact ttaaatatgc aagctggcat aggccagaag cgccaatgtg 63780

ttgatgactt atttgacctt ccgccatgtc atgaaaagaa aactgctctt tctgttgata 63840

tgctttaaag ttcatgttac gatttgcatt ttaatcaata aacagtttaa tttcatgcgt 63900

ttgtattttt aaggtgggag ggtcttgaaa agtacggtaa cacctacaaa cattaagatt 63960

caataaaggc gtgcgttgca gttctcaaac actatctgga aaatggaaaa aaatagaggt 64020

gccgataggc agcatggggc cgctgcggct aggcaacgtt tactagctac atttgggcaa 64080

ctacaaactt acatatttca aatagaattg cttaagcgat gcgaccccga tgttatactc 64140

ggcggagaaa ctttgcgcaa cttaaagctt aatgctatga tggtacgtta tctggaaaaa 64200

cacatcaaca cgggattcgt aaagcaagct gagatttatc tcacgccgat gacatacgca 64260

cttcatctat ctttacgata tgcaaaaaag gaaggtgagt cagtactagc tgctatagaa 64320

tcgtatcgtc tacatgggaa cattagaaaa ttcttttcag atacaatggg cctggcgggc 64380

gactgcccat atcaccgcgg cgtgtcgctg cgtacgtatg ggggtgaggt taggacagaa 64440

atcaaatttc ttcacgacgt ggaaaacata ttgaaacaat taaacttttg tcatctaata 64500

acaccagtag acgaagcaga atcatttttc gacgcgttag atggtttttt gtcattgaca 64560

atcggttcgg gtttcgtggt gccgcctcaa ctattcgacc catctcaccc atgttcggta 64620

tgctttgaag aactgtgtgt aactgcaaat caaggggagg cgatacatcg tcgcatggcc 64680

aataaaattt gcgaccatat aacaaagcaa atacgggtac gtattgaccg tgaagaaata 64740

ttacgccatc tgccgtacat agctggtcta gacccagaac gaaaaagggc tgctctggca 64800

gccctagagg ccataggaca gcgtatgcgc aatactgata gttcatataa ccaaacgaca 64860

atcgatacgg cagatgctat attagaagcc cataacgttt ttttaccagc ctccaaaaga 64920

ctatatgcta tcagtgaact tagatattgg ctcgcatctg ggtctatcgg caatactgac 64980

aaccaaacat ctatgatgtc cggaactatt agtgctttcg cggcaaattt ggatttgttg 65040

gcggagcgtg agtgtagact cgatttagag accgcagcag tagaactagc actattcaat 65100

aggactatga tgcattttga taaggcatat gctgataaca tggcagaatt ggatgtcatt 65160

gattgtcttt tattgggtag taggtccgcc tcgccggatg accagataga ggcgttaata 65220

cgtgcatgtt atgatcacca tatgtctgca ccgttgttac gtagattagc tgacccagac 65280

aaagctaatg aagatgcgct gcgtaaagtg ttagagcgcg cgtctaaaat gaactctcaa 65340

aacaatgggt ctaatcgcgg aggagataac ttacaaacta agaatacatt aggtattata 65400

acagagtcgt cagatagttc tgatacggat gaggatgaac agcggaaaag tataactcag 65460

cggtacgatt tgaatacaaa tatcagaaat acatgggaag atcttatacg aagagttgat 65520

gaagatacaa gagagcgacg aaaactatat tcagaaaagt taacaaaacg gtcacttgcg 65580

agcttagata gatgcgttcg cgaacagcgc cgcgaacttg agaagacgtt gcgtataaat 65640

gtgtttggcg agacattact cagcaccttc gtagcggcgt ataatggttt tcgctcgcga 65700

gctggggtgc tgaccaaagt cataaacgcc gccggaactg taatcgataa ccgaaattgg 65760

gatggtgctt ttgacgccca tcggttcatg cgcacatctt tactcagaca atcaatagac 65820

cctgcaatgc tgcctagtct aactcataaa ttcttcgaat tggtaaacgg tccattattc 65880

tgtcacgata ggcacatatt cgctcaaccg ccgaatacgc cattatattt cagcgttgag 65940

aatgtaggtt tactgccaca cttgagggag gagttggcca gatttatggt aacgcccaga 66000

ggggcggact ggacaacgag tacatttcag ggcttctacg actttagcgg aattgaaggc 66060

gttactgccg cccagcgcct ggcgtggaaa tatatcaggg aattaatcct agccacggca 66120

ttattttcat cagtcttcca ttgtggagaa gttaagctaa gccgtgccga tcgagcatct 66180

cccgggactg acggcgttcc tctatgtacc gacggactat atgttactta cgagacagaa 66240

tgcccgctta tttctgtaat tcgagcgggc cagaatggag aaataacccg cgggacgttg 66300

gttgtgatag acaaggacgt cttctctatg ctttatacgg tgcttcaaca actggctcca 66360

gaaacggcgg agcgtgcaca acagcagtca gaagaaagga gttcgacaga atgaaccgcg 66420

cgcgccgagt gtccgcattg cacctgacag cccctcgccg gtcacggttt gttctatcgc 66480

taatcatggt aagcgtattg ttcagaccaa ttcaaccaca acctattgta aatgcaactg 66540

ataggccgca tggactgatg aatgatcaag atacacatct agatggtgaa cgactgcaaa 66600

gaggtaaatt atcggcccgt gaattgatca gaggggtgca gtcgacgcgc gaagaggaca 66660

aattctatgt ctgcccccca ccaactggca cgacggtact acgcttcgaa ccatctagaa 66720

aatgtcctga agcatacaaa gggagaaatt ggaccgaagg cgttgcggtc atattcaagg 66780

aaaatataaa cccgctaaag ttcaaggcaa tgatctatta caagaacgta atacaaacta 66840

ccacgtggtc tgggacatgg tacaaacaga taactaatcg atataccgac cgcgttcctg 66900

ttgctctcga tgagataacg gaccgtatag atcgatttgg gaaatgtcta tctgcagcta 66960

agtacttgcg aaataatgtg tatgttgatg cttttgacag agatgagagt gcaaaggaag 67020

taccgctact agcttcacgt ttcaacactc cagactcaag ggcatggcat acgactaacg 67080

atacgtacac agtgtttggg tcgccatgga tataccgtac gtccacgtca gtcaattgca 67140

tagtggagga ggtaatggcc aggtcggcat ttccatacga ctacttcgcg ctcgcgaatg 67200

gagacgttgt caatatgtct ccattctatg gctatggacc aaccgaatct agagaacata 67260

acagctattc atcaaataga tataagcaac tagatggata caaggtaatg gacctggata 67320

cccaccgcct agcttcccca attaagagga actttcttac tacggagcat tatactgttg 67380

gatgggattg gatgcctaag cgcgaacgcg tctgctctat ggctaaatgg caagaagtta 67440

atgaaatgat gcgggcagaa tatggcacga gataccgatt tgcatctaaa acactttctg 67500

catctttcat cagcaacatg acgcaattta acttggatag attgtatctt ggcgactgcg 67560

tgaagagaga ctcggaagaa gcgataaaac ggatatttga agagcagtat gcatctactc 67620

atgttaaagc tggagcaata gagtattatc taacttcagg gggctttatt cttgcttatc 67680

agccagtagt tagcaataca ctggtgaaaa tgtatgttca agaactgacc cgagataatc 67740

gtacagagtt agctttagac cttttggggg caatgcgggg ggacaagacg cgcgaagatg 67800

gtaggagtcg acacgcccgt agtgtagatg gagggcctga cggtctaata aggggtgcaa 67860

gaaatataga tccgtataaa aactatactg taagatctgc aagggttgcc agaaatgcgg 67920

acccatataa gaacactaca gtcgttaagt ctgtaatgtc ggcccagttt gccatgcttc 67980

aatatacata cgatcatata caagcacatg taaatgatat gttgagtcgc attgcagtat 68040

cttggtgtga gttacagaac aaagagagcg ttctatgggc agagatgcgt aaggttaatc 68100

caagtctaat cgcaagcacg actgtaggac gacgtgtcgg agcgagaatg cttggcgacg 68160

tattagccgt ttcttcatgt atcgaagtac cttcgtcgca gatcagcctg tccaactcaa 68220

tgcgcattcc aggaggatcg tcatgctaca gccgaccccc ggttacattc tcttatgaaa 68280

aagacggcgg tacaatagac ggtatgttag gagagaataa tgaattgttg ctgactcgcg 68340

aatataaaga accatgcgca gctaatcaca aacggtactt catgtttgga cctaattatg 68400

ttctatatga agaataccaa ttcgtacgac aagtagaagc ggcagatata caaatggtta 68460

gtacattcgt agaattaaac ctcaccatgt tggaggatag agaaatatta ccccttcaag 68520

tatatactcg tgaggagata cgcgattcgg gcgtgctgga ctacgccgag gttcaacgtc 68580

gcaaccaact acactctcta aggttttacg atatagataa agtgatagat atagactcaa 68640

actatgcatt catggccgat ctgacgaatt ttttcaaggg acttggtgaa gctggccagg 68700

ctatcggtaa agtggtggta ggggtagctg gtgccgtagt atctactgtg tctggaatat 68760

catcactact gtctaaccca tttggcgcat tggccatagg tttaattgtc gtggctggtc 68820

tggttgtcgc atttctagct taccgataca tcagtaagct gcgtaacgac ccaatgaaga 68880

cgctataccc aatgacgatg aaaacattaa aaaatgaagc caaacttact cccaggggtg 68940

aagcggatgg tgaagatgaa gatgaatttg atgaaagtaa attggaacaa gcacgtgaaa 69000

tggtacgtta tatggcactt ctttcggcct cagagagaac agaaaagaaa gcgcgtaaga 69060

aaaacagccg tactgcacta ctttccaatc atctgtctaa cttacgatcg aggtcaaatg 69120

ggaaaaagta ttctaaagtt gaagacgaat atgaagatgg cgattcggca gatgaaaccg 69180

aaattcttgt cacagacaga gtttaattat tatgctttgt atcaataaaa attatcccaa 69240

agctgttgcg tctcattgtt gttattgtct tccctccccc gtgtttccat ttttccacga 69300

cggtcgttat tggacccacg aagaaaccgc ccaacgaaac tgtacgtgtt ttaaatacga 69360

gcgtatagtt taatcgggag gcagcttcat gtgccacgag taattgctaa agttgtcata 69420

tttgccaggc cgaatgcatt cttgccgtat gaattgcgct gacaaaatct ccgtttgaac 69480

attctggact gcaatccgca ttagaccggc cgggcagtac agaacgttca aacggaggcg 69540

ttgtgcgact ctcggtgtac gccggcatat tcatgtatag acttgaagat gctcaaaaga 69600

aaaggcacgc aaccatttaa tcgatttaat tttattgaag taacaattaa caatttagtc 69660

aacatctatt acacatcatc tgcgcgataa attcttccgc gtcacgatca actttacatt 69720

cagtagttcc tcctatagcc acgaccgcac tcgcgtctac attttcggtc tgaattgttt 69780

gtgccggcgg gtccgcttta accatatcac ttgttgtagc actctttgta tgtggctgcg 69840

cttgctgagt tgtcattggt tgtggtgggc cggcatttat ctgttcatac gatggcatca 69900

cagattttac cgcggtctgt ggttgcagat cggcttgctg aggttgggcg acattcgcgg 69960

tctggacggc ctgcgtttgg cgtagacgcg agacctcatt atggagagac acaacagcac 70020

ctagtagatt acaaatcgta tccgatggca aagctaatgg tccagtagac gtagcgttgt 70080

catcgttacc ttgtccagta ggcaccactg gccccgaata ctgagggggt gttggcatgt 70140

attgtttggt ttgctgcccc aatggttgtt ggccaccgcg tatagatccg cgtcccaaat 70200

aaactgattc ccccggataa tagatattgt caccatctaa ctggtcgtca tttgcatacg 70260

gccgttttct tccgcggcgc tggttcataa atacctggct ttggtcaaca ccgcctgcgt 70320

ggtgcggatc tccagattcg acggcttgtc cgcgcctatc tgcagcaatt gctccgatga 70380

gcgctgcaat ctgagtttcg agggaactcg gttgcgtagt ttggtatggt ataggcagct 70440

gccctccctg gacgaatggt gcctgttgaa aataaacagg ctgtccatat atcggtccag 70500

gatagggcat actaccagct gtcataaagc gatgatgctg ctggtcgttc atttgtgctt 70560

gccatgggct ttgagatgtt tgctgtaaca cttgattgtt aattccagtg ttgctccctc 70620

gttggttgct gaccaaattg ttatattgcg atactggcac ccatatgtaa tcgtcgtttt 70680

gtccgcgaga tatttgtggt ggatgacgga taatattgct atctgtcgtc atagtagatt 70740

gttggcggcg cacgatagac gggctagaac tcaaagccag attctcaggc aaaagagttt 70800

cttgcccgtc ctcctgcacc gactgacgaa tgcttccagg tagttgtgcc tcaacctttt 70860

taataatatg acgtctctca cagacgccgt tatactcctc ctctttcgat gtttgaccgc 70920

cgaagccgca gcatgccgaa gcttgcaaat atgtatgacc acgaataccg gcctgtttgc 70980

gccgttccgt aactacacgc catctatctc tcaaaagcat agtatttacg gcagtaccca 71040

ataatgcgcg cgatatagct ctatcacttg gtgaccatag tgacggaaat aatgtagttt 71100

ccgcctctgc agattcactc aaaacggcgc ggcgcgccgc gtcggaaagg tcacgaaacg 71160

gtaacagagc attttcggga gaaatatcat atgtaactat tgtaccaaca cgtctgccaa 71220

gtaagcaaag cgctacatga gaaaaaagtg acgtatccgg ttcctcgcca gcctttaatc 71280

gtctagatga tagagatgca gatggtaggt agtttgtaac tatatataac aaacgctcct 71340

cgggtgttag ggagagctcc ttgtttccaa aaatttcgcc actagccgca gataaaagga 71400

ttgttgctag tattggacag ttgatcttcc cgaggaaaaa tagtcccttg tccgggtcat 71460

cgacgattgt caatacagca ccaatgacac aatcttgcct atgatctatg tttattggga 71520

gtggtgccgg tataggtatt gctgctgtcg cgacgctacg atccatagtt aactctcccg 71580

ttccatctcg cccgtaaagc gctagatagc cagcaacata cagcggaggg tacgagtccg 71640

gactattcga aaacgatgac gcgtctcctt cagttctgtt taacaaattc tccctaataa 71700

tttcggtcga ttcgacaccg gaattgttgc cacgatcatg atcatcttgt atatcgtaat 71760

taattgcagt atcgtcgtcc atgacacgta ttttgtataa gatcgtccta ctgtcataca 71820

atataagaag taatcaccgt gacagtcatt cgtgcactgt cacattgaag acgcataaag 71880

tggaatatag cccagacata agaaataaat caaatcataa tcactgttaa cattgaattg 71940

taaaagacgt ctcccgtccg atagcgtgtt ttttagtcgc gcctgttgca tcaatgtcgc 72000

caaaccttct tcatactgca aagctaccgc atcatgtgtc gttagtacct cttgaatagg 72060

tacagatata gtcttagacc tgttttgcaa ttcgaccgca atgagtcgca aaagggcagc 72120

ctgggctcta ttcgttgaca cgtctaagac atggcgtgta gcattttgtg tttttatgtt 72180

acgtgcatcc aaacaaagag ctacgagacc aggaaacatc tgtgttatct cagtttgcgg 72240

atcagctgca tataatgaag ccatataacg ctcgcaaagg aacgcgaaat tgcgattatc 72300

gcttttcatg gtttgggccc ccgcggcccc gccattggac gcgccgcgat ttaagcgatc 72360

agatggtact gcacctggca acaacgttcc gatctgaaaa ttattacaca tgcgatccgc 72420

atagacattc gtattccata ataattttcg catgagtatc agtgatgtta tggtatttat 72480

agtggcgcgt agctgagtta tatcctccat aagaaataga ggttgggctc tcccaaatac 72540

ggccccggca gctttattta cctcatctac ataagctcgt tgactcttat caacatccct 72600

cgcaagtcca tctggggcgg ctacgccagg agacagcggc aacacaccat gttttataag 72660

catagctaga acacaatgac cagaaagggc aattcgttcc ccacttctgg gttggatggc 72720

aaactgaccc ttaggtaatc tatccatatc aaacctataa cacaagcgac cgcaatcgga 72780

ggcgagacgt tctataaaat tatccaaata agcaggtagc tgctccacca ttcctatagc 72840

actagacgcg tagttaaatg gttggcattt agtcttggca gatgtaatcc tgtgatgtag 72900

ctgtaggcat aatatcgcgc actctagagc tgtataatga cggactccta catacaagcg 72960

cccagtggcc tgtagtgata ctatagcagt agtcataaac gttttggact gccgtccatc 73020

gcgctggtct aaccgttttg acacaacagg ccactcagta atcaaaccgt cctggagagc 73080

tctataccaa gtaccgaata atacacccca accagaagcc gaagagccac cacgtgttga 73140

ataaataata gttaacaagt ctgtactcaa attagtgtca tagcgcaatg gtgtatcgtt 73200

cttaactatc tgtacttcgc gcctttcatc attagaatca cttgtttctc ccaatttatc 73260

cccatcgccg tcttgtcggc tacgttcata tgatccatcg atctcatcag ccgcgtccgt 73320

cgcttctgcc atagcagcag cattctccaa atctgcgatt actgctgtta cagattttac 73380

ttgctcttct ataggtttta aacgcatttc taattcggct gatatacagt tattcttgat 73440

gcttaaatga tccaacgccg ccgatgccgc agtattccgt tgttgtaata tacgtaattt 73500

ctgtatcgct gtttcttctt catggcgatt tgcctgcgat tttgtccagt agtgcgctgg 73560

ccaattcggt gttataaaat tcctagaatc tgatatgtag cccgcggagc tatgttcttt 73620

gcacatatca agtggggaga aaaagaatct ttccattgtt gattatacaa gccgatagtt 73680

ttaagaaatc caatacactt ctaatcgaca gaccctcgca cagctagtaa caaaaaccgt 73740

tgtcaaacaa ttctttttaa acactcgcga ccagaactcc cgcccataat aatcaacact 73800

gccagacaat aggatggtaa tatgcgtttc tgtaataatg cactagtgtt tagttggtct 73860

gaatatggat gctacaaatc gcaatactcc accgcctatt tgtgtctctg tatcgctaca 73920

attaatatca ctgtctgatg cgacgccatg cttgccatca taaccgtatt ctccattaat 73980

accatctata ttcgttttgg gttcttcagt attgtttagt cctgtatttg tctgtggtgt 74040

aaccaactgc ccataacccc atagtcgcaa tagtcgtacg ctatggatcc cagttgcatt 74100

gccgtgtggt atgttctcgc ctgccgcaga actcgtcccg acgcctggcg aaaattcttc 74160

ccaccagcgt tttttcgcaa gactggaaga attttcgctg ggtgtacgag cacatgtcgg 74220

gttcataatg gcatcatcga tgtcgtcttt gtcgacaatt ttgcacgcat ctttactgta 74280

taattcggac tccaattgaa catactggct acatatacat ttagccttcc ggcgcttacc 74340

tttggtaggt atatgatatt ctgataaacc tgcaatagag gctgataatg ccgcaaagtt 74400

ggaggatacg cgccgatgag caaatcgtgt aattcggagt atgtccagac cgcgctgggc 74460

tacaaatact agcaacggac atattaaaaa ttgactgcat ccagggggca tgatacgttc 74520

caacaagatg gccgattcca tcaattgtct aattccagtc catgtttgct gttgtttact 74580

tcctgttgtc atatttttta caaacttgca agtcttgagt tccaaaataa tacatatgac 74640

atctgcagtt tctgcctcgc ctgtcttaat catgcatatg caatctgggc ggcgccttcc 74700

taagttgact tcaaaagtca gcctcacatc aaatgcagtt ttaagtgtcc gcaaagacag 74760

agtgtgttcg aatagccgca caatcagttt actgggcgat ccaccatttg aacgaaattt 74820

gttcaaatcg ttagccaggg cgccataaaa acgattgtgg cagcgcactc cagctttgag 74880

gcgacgtgcg actgaaccgg acgtgttggc atcggttcgt ctcttggatc ctgctacgcg 74940

tctacaaggc cgctgttccg cgttcttaag tgcggctgtc gtcgctgcgc caatacattt 75000

ccgatcaaat gtcactgcgc gactcttgcg aacgcttaat ccggcgcgct ttttctgtac 75060

cttcgatacc attatgcctc gtccgcgtat acctagacgg gccgtatgga actggcaaaa 75120

ccacaactgg caaactgttg tcggaggata cccttgcagc cactctttat gttgccgagc 75180

caatggcgta ttggagaaat cattttgaag atgtaataaa gggcgtatac gaaacacagg 75240

aaagaaaagc tcgaggtgac atagcaacaa ctgacgcaaa agcaattacg gctgctcttc 75300

aactgcaatt tttcgcacca tacagttcct ttcatacata tgcatcgacg ttatttggag 75360

tcgaagcgcc agaaagaaca ccaccagata ttacgctcat aattgatcgg cacccgaccg 75420

ccgcatgtct ttgcttccca gcagctcgtt ttgtagtggg ggatatgagc cttagtactc 75480

tattggctat gatatcccta attcctcgcg aaccccatgg tggaaatata gtgataatgg 75540

atttgaatga agatgagcat cttgaacgat tgagagctcg ccaacgtcct ggcgaagcaa 75600

ttgacgtacg cttcttgcgc gtattacata atatatataa gatgtttgtt aatacaattt 75660

ggtatgccaa acaagcacca ataatgtggg actgtgataa atgggacaaa gattggagct 75720

cggtcccaat atttgatgac gatagaaaga aaatgttaga agcgcgtgat tcagtgccta 75780

ttgttggtgg gaaaaaacgg ccaagtccat caacgactct cttgtcactg ttcaaagtac 75840

ctgaactatg tgacgaatca tttagactgc gtaaagtcca tgaatggaat ctgagatgcg 75900

cattaattaa gttggcggat ttgaatgcat attttatgga cataagtggc aagagccccc 75960

aagagtgtgc cgctgaagta cgagaggcaa ctaacgcgat ggactttacg cgcctatctt 76020

ttaccatggc cggggatctg gaacaagctg ttaaccaata caataccgag atgacaatta 76080

attgataaat atatttgttt taaataaagc tctgatgggc ggttctgaaa ctatgacgca 76140

tatgtgtgtt tattgcctgc cacatttgta aatgcacgcc ttcgctactc cctatttgtt 76200

ttactgtttg gagacagtta gtatatgttc acgcgagaat ataatgcgcc attccgccga 76260

gaggacctag gcgagctttc tgggacctca ccgcggagct agttttctct acgcggccgc 76320

ggttctgtcc caggtaaagc taaccggcca actttagaat gtcgcagctt acggtgctaa 76380

tatatacagt catagttttt gaggttgtaa gtgcggcgtg gataggtgtc ggggaagaag 76440

atatgagttt aaactttact catcacgtta tatcgccaga tgttcttcgc catgccttta 76500

caggaatagt aacggacagt ggaccaactg ggcgtagcac atctacaatt catgtatcgt 76560

cggttctcgg cgcgccgtac ggaataaatt ccaccgatct gagtaatcta aaatggatac 76620

atgcaaccac aatattcttc ttcgttacca accccaaagg aacgaagttt acaggtactt 76680

tgctattcgt acctagagaa gcgggcataa aattcagatc aattgactat cctgacagcg 76740

ttatgcttgc gtcgcccaat tttgcacgtt ctctagcatg ttctgcaacg ccggataatt 76800

tatcagaata tggaaagata aaacttaaag aacacgtcga cgatataaaa catgtacgtc 76860

ctgttcggcc caagcccagt ggctcaaatc ctccaaagga cattgctaaa atttcatttt 76920

ctgacatact ccgtccttcc gaagcatttt ttgatcccaa aacgctccta ccatcagcac 76980

tggaaatcac acgcttcgtg gatccactgg cctcagtttg gccagtagac tattataaga 77040

cggcggaatt acgttttgga actgatcgcg cggaagctgt ggccaatatt ggagacacct 77100

ttatgacatt gaccctctcg atggccagtt cgcgcccaat tgaaattcta atagcgcata 77160

ctcgttctcc aatgtttatg atatggcctc gtttcgatac agtattcgaa ccgccacatc 77220

tcagcataag cgacaaattc aaaacataca tattggcaag tttcaaaaat tctgccgata 77280

acgaatttcg agacatgaca gaacttggag ccattacagc agaagtgttg gagccacaga 77340

accatatatc agaaggacat ctacaacttc tattaatgtt atccaatgca caagaagaag 77400

acgatgttta tttctggaga cgaatcgtta cacgtgttgc tttgattgca ttttctttca 77460

taactgaatc aagttcttcc aagtttacaa gtctggagga ctacatagca acggacttaa 77520

atacacgctt ccttgcaacc gctgtgtgta aaactgcatt aactggagac cctgatacat 77580

tttttatgcg aactggatta acattatccg agaaggctac cagtatatta actacacggg 77640

tcatagaatt ggtagacgac atacatcgta agagtgatta tggcaagcgc gaaccactag 77700

aagaagtact aaatgtactg cgccttgggt atggtctggc cggcgataaa cgcctagact 77760

ctgcactaga taagctctca acatcactta taagtaactt atattccgac atcgtaatgc 77820

tgcacatggg atggaacgta acagccaaac acacgttgtt ttttgcaact gagtccacgc 77880

tcggcagctc cgtccctagc atggtgaggg ctcgttttgt acttctgcta ctaacatcaa 77940

tgtgtactga tatacatgcg gcctctataa atgtaaacct gcaagaaata ttcattagcg 78000

ttactgatcg cggcaatgat ttcagtatgc tggacctatt ttctccatgt gtcacagcat 78060

tgcgatacga cacacatgaa ggaatacata aactatacgt gcttagttca attcctgata 78120

ggttacgcct cgtcgcggct ctaaaatcag acaaagatgg aggaatacat aaagaagata 78180

acctaaatat gcgtgctgag aggactatag aacgctggag gaatcaggac ataactgcga 78240

taaaacactt ccttcccgaa ttatttgact gtggagaaag gttaaatctg tcgccagata 78300

atggccttgt tatgttgctg ccattgaccc ccaacggcac ttttattatt tccagacatt 78360

tcctagacgt cggtgtaaca tatcgcattc taggcgtaga tgttaacaac cctttatata 78420

taacatttaa acatgccggg tgttcgtcct ctagttccaa tattgcgtca gtacaacttc 78480

cgcgaccagg aacaaataca gagtgtctgt actgtgggtg tgtaatcatg cgttatacat 78540

ccaccggcgc ggttttgtat tctgtatata tagccgatct ggccatgcag cgcgaattta 78600

tggccgggat aaattcttcc actccatttt tcaatcctgc atcatttggg ggtgcagaat 78660

cattactatt atttcctaac ggaacaatag ttaacatact tgccttcgaa caaaaaattg 78720

agtatgtcat accgggcacg tatatcgcga tagcggttgg gggtatggct gttgccgtac 78780

ttgtctttta tgggataatt aaactgatgt gtaatctaat tagaaatggg gacttaaaag 78840

aactgttaag caatgaagaa tgattttata actcaatgca gtctgttaaa tcaatttatt 78900

gttcctttaa ataaattatt aaacagtata taattttgcg taatttcata accaaaagga 78960

tttcggttaa tctaatacat attgcccttc gacccatcat atacgtccac attgcctcaa 79020

gtgtttgcgt cattgaaaca cacagactat cattaattat aaccaatttc agtaaactat 79080

tcaaatatat gatcccgtat acataactga tatattagaa tctttactga tataaatact 79140

aaaacacatt accctaatag accggcgttt tttaagcggc gatcaattaa cgcgccaagt 79200

gtcaaaacac ccttttgaat atcacatttt tcatcaaata ttgacgagca catcgcgtca 79260

attgtagcgt ttaatatata cgagttccca aaacatctat cagtgcgtat tgccgtcttc 79320

attaaatcat tagtcgtata aatttctgcg attattgaag cgagacgcgc tatacgttgt 79380

aattgaatag actggtgttc ctgggccgtg tccaggagat cggtagccat ttcaaataac 79440

gtttctgcat catccatcac cgaatcatct tcatctgttg caacgcgcaa ctgtttgacg 79500

cgcgttgata atatagcgac ggaagatgcc cagattatgc aagattcgcg aaaaagtcta 79560

tttgtcgtat ctactagcaa atgtatatct ctgggcggtt ctggaaacga ttccgagtcc 79620

gcgcttagac ttacgcataa atgctctatg attgggtaac aatttggata gccgccttta 79680

cacaatatat actgaaccag cgcaaacttt tgtgcgctag aaactgaacg tcccatggaa 79740

taaccaacga atagttcccc aatgttgcca aaagatgctt ctattgcatc gccaattcta 79800

gctctagcgt cattaaattc gggttcgaga ccaaaccaag tactaatatc ctgagagact 79860

acatccccaa tcctacaaaa tacggtccaa agggccccaa gcgatcgcgt aggtttgaaa 79920

ttaccggcac catattccca tatattaagc acagcgggaa tgtattttac ttgcacaaat 79980

gaactgacag tagttttgcg tgcgccgcga cgtgaatgtt ttcgagaatg aagtgtggtg 80040

gtaatgtcgc agtcatcatt tgtgtttaac gttccctccc cagagccgcg atgtcgctcg 80100

ccatgtccat gctttaccaa tactgttccg aaggagcgct tgccagtaat tattacatct 80160

gtttgttttg tttcttctcg tactccccta cgtcgaacgg ctatgcgatc aaatagtcca 80220

tcgaccatag cggcaagtga ctgcggcaat gatgtaagac gcgcctgcat gagcgcaaat 80280

gcattcctat ttggttcgta aataaaacca gaacttgcat tagtaataat tggtacatca 80340

tataatttga taacttgata gttcgaaaat atatgacccg ttgataatat caggcctgac 80400

gatatttcga gtgaaaccat atactcagcg atacactggt cgcgaacttc aacctcaagc 80460

gccccgcaca gatcacgctc agtacttcca tcaccgctat ttgtcccgca gagatcgcgt 80520

tcgctacttc cagaagtatg cccgagtaaa tccaaactat ctataaagac ctcaccatca 80580

ccaatgatcc atttcaaccc ccgccgtcca agttcacttc ttacataatt tgacagtacc 80640

aaatctccgg aggttatccc tcgtaatgct ataccaaact tggccaattc ggcttgtgtg 80700

tcactgtcat tttccggcgg gcgtggtact gataatatac agccaccaca tgcgaagtat 80760

gctctcatgc cgtcgcgtgt gatatagaat gttactccat tgtggtttat ggtctcccaa 80820

taatgaaatt ccatcgttcg ccgcaaaggt acttccttaa agcaaaagta ttcctcctaa 80880

caacctatta tactataaag ttaacctatt agaagcctaa ccagtacgtt cctggatgtc 80940

gaatggttct aggctaagcg acaagcgtca tttaccgggg gcacaaacgc ccaacaaaat 81000

cacagcgacg acacgtagac gcagtgggcg gtcctagatg tttatttcgc tattttaccc 81060

cccgagagaa tattaataaa caattgtcaa gaggattatg tgtgaggact atttatttct 81120

ttgtcccttc aacaacaaaa cagttaacaa aaaatagcat accaaagtgg tttggacgca 81180

aggcagctat tggataatac attaatctta gtacctcctt taaataccag cgcgtttgat 81240

attgggccaa cctcgagcat actcatctta acctttacaa ctagatatgg cattcgatgg 81300

aagacactca catgcaagtc ccatctcgga aaacacagaa atgacttcaa acagaaataa 81360

cagtgaagca gtagatagta atgagatcgc gacatgcaat ggtggtgaca gatgttcgga 81420

aataacaacg actgtcaatg aagcaacgga cataatagac gacctagaaa gcgttactga 81480

atttatggca ctaacatcat acggctttga cgcagacata tacagcactt cagcattctc 81540

tcaagtaccg gcgtctgggc atcctgtttt cacacgttat gtagtaatat tttggctatc 81600

cacattaata atcaagcctc tttgttgtgg agtttttgct atttattatt atattaccgg 81660

cgattacaga tttttggcta ttggactcgg ggcgacagct gcattctacg tacaactgtt 81720

tcttactgta ctgtttattt atatcaacgt acgcaaggac ctcttaccgc tgaaaggttt 81780

gcagcgcata gttgtcggta cgatagcgct ggcatgtccc gtcgttttcg cagctactgc 81840

ctatacgttc atcttccaac aacctgaatt cttcgaccgc atactatatg cctcatttaa 81900

agacgattcg ggttcatcgc ggctggtcca tttcgaagaa gcatatggac ccataagagt 81960

ggatgctact ataggtgcgc gcggcgcgtc gtgtattact ctaatagcag taattatata 82020

cgcaactggg tcgctagggg acgctttagg tttcctactt ccgcgtcttt ggacgagggc 82080

ggtaatgaaa acctgcgtgg gattttgaac gcactttcgt catcgcctat ataagccaca 82140

tcgagacggc caacatttta tttccctaaa gcggccagtc agtggctgcc gtcagtacat 82200

tactgataaa gactactaaa tttctttcga tcatataacg cactgtcaac gaaataatcc 82260

tgctccaaag atggataatc aacaagagag aggggagaca cgttttaatg ttggagccag 82320

ttatgcacca acgggacgcc ttctcactac aatagaggtg tcgtctcatc gcactctgtt 82380

tgattttttc cgccgcttca gttccgattc aaatactcta tacgaagcac agatggacat 82440

gcttctcggc gtatactgca atacactgag cttggtgcgc tttctagaat tgggtctttc 82500

tgttgcatgt gtttgtacaa gatttccaga acttacatat gtgaatgatg gtaccattca 82560

atttgagact caacagccga tgattgcccg cgatggaccc catccagccg atcagccggt 82620

tcacaattac atgacaaaaa gtctcgatcg gcgatcactc aatgcagcat tctcaatcgc 82680

cgccgaggca cttggcttac tatctggcgt ttcgttcgac gggacccaaa tagctgaaca 82740

tctacgcaca agggccatac aacagttggc caggaacatt aatgcagttt tagattcatt 82800

cgagcgcggc tctgcggacc aaatgctacg tattttattg gaaaaggcac ctcccctagc 82860

tctattgggc ccactttcac gttatttaga tgaaggtagg ttatcaaacc aagttgcgcg 82920

tgctaattta atatcagaac ttaaacgtcg tgtatgcgag gatagttttt ttatgagtaa 82980

atatgtcgcg aacagagatg ctataatggc gcaactttct caactagtca gctcaacgcg 83040

cccgtcagta gccgtacaaa gactgacaca ttccgatacg tctggcagac cagtagatgg 83100

tgtaattgta acaacagcga cagtgcgcca gcgtctacta agtttgctaa atatggccga 83160

taatcatgcc aatgtccccg ttacttatgg tgaaatggtc gtgactggta gtaatcttgt 83220

aactgcagta gtcatgggaa gggccgtacg caatcttgaa gatgtcgcgc ggcaccttct 83280

taatctacgt gacgatagga tattgaaaga taaagatggg ccgatcaata cagatgatga 83340

cgacacccca cggatggcac gtgttcgtgc tgatcttgct tttattggag aaaaactggt 83400

attcatagaa tcgttggagc gtcgtatata tcaagcaaca aatgtgtcat atccattaat 83460

cgggacaatt gatttgacat ttgttatgcc ccttgggtta ttcaaacctt tacaagataa 83520

ttatgcacgt catgccggtg attatgcccc aggtgcgggt caagttgata cgagatcgtt 83580

tccgccgagg caaatactat tttataataa ggatggacaa cttatcaaac tgacactgga 83640

agacgccatc ggaactgtat gtcacccttc atttctggaa attgacatgc cacttgattt 83700

cctacgagca acaccacgag acctgcgttg tccgttcggg gcgtacgtgg cagtccaaac 83760

tcaagaagat ttggccgtgt ctatgcggcg tttttttgac cgctggcttg attatatgcc 83820

agatacacct agctgggtgg cagtggagac ctatactgtt gatcaaatgc tatcacctgg 83880

caactctcgc ctaccttttg aacttcaccc agcttttgac ttctttatag cccccgccga 83940

tataaatttg cccggtccgg caaacattcc gcaggtcatg gcaacggtca atgctacgtg 84000

gcgcgtatgt aactgcaaca tacctctacc tctgtgtcct tcaaacttta gagacgccag 84060

aggtcaagag atatgttcca accgccatcg catgagtcaa gctactattg atgccgtccg 84120

atctacattt gcggatgtta attatccaac agtattctat ataattgaag ctattgttca 84180

tggcagcgaa cggatgttcg gaatattaat gcgattaata atgcaatgta tccgcggata 84240

ttgggcaaat gggcgccgtg tcgcatttat taataatttc cccatggtta tgtatataaa 84300

cacctatttg ggaaatggtg aattgccaga agagtgcgct tctgtgtatc gtgaaatagt 84360

cgatcattta cttgctctgc gcagggtcat aacagattat acattgcatg ccgataacat 84420

tggccgccag ggacaagaag aactcaacaa cataatggcg gatcaatcgt ttttaccacc 84480

attaatttgg gacggagatg cattaattta tcgctatcag gcagagcacg atagaaacgc 84540

ggcaataata gtaggtggag aggagggatt cactctacgt cccttttctg gactgaatga 84600

tattaacttt gatagaacag gcaacagttt aatacacaac gcccatattc gtgccgacgc 84660

cggcaatata caacacactg aaccgcacca cgatgcagag tgggtaatac taaacaagct 84720

ctattacttt gtgatggtgc caacattttc aaggggccga tgttgtacca tgggcataaa 84780

ttataatcgt gtatatgcaa ccgcccaggc ggtaattata ccagatttgg cagcggacga 84840

agaaccacca attggacccg atgatgcgag acatccactg aatgcgcgcc atctcgtacc 84900

caactccata aatgtactgt ttcataatgc aagaatagtt gtggattcag acgcgctgct 84960

gacattacaa gaatccgtcc acgagatggc agaacgtact acggcaatac tatctagcac 85020

agggccggat tttggggctg ctactgtcgg gaccagtctg ctgcgtacat acgatggaac 85080

tctgcaccat ggactattaa tgatggccta tccgcgtaat gatgaaactg tagcagctgg 85140

aacttttttt tatccagccc ccgttcatgc gctctttgca tattatgagc atattgcagc 85200

atttaatggt ctgcccaggc gtgttcatca aaccgccaga cgagtacctc ccgtacccat 85260

ctttctaggc gcaaattact atgcaaccat tcgccaacca gtgttacgtt atgtcacgga 85320

aagcagagcc gacgagaatg ctctttccta tgcactttta gctggctact tcaaactttc 85380

ccccgtatca ctactgcacc aattgcgcac tggtctgcac cctggatttg ccttcactgt 85440

cgtcaggcaa gatagattcg taactgaaaa catgctgttt gccgaaaagg catcggaatc 85500

ctacttcatg ggtcagctac aagtctctag acatgcatcc gccggcggaa ttacatttac 85560

actaacacag ccacgtgcga atgttgacct tggtaccggt ttcacagctg tacatacgtc 85620

ccccctactg agaacaccgc ttacggacat gggcaatgct acccaaaatc tatttttaac 85680

cagggggtct gtaccaatga tagatgccga cgcagatgat tttattaggc gtggtgcgac 85740

agctgggcaa agactagccc cgcgctatca agtaccgttt atgggaacaa tactaccatc 85800

gcagccatct gggctggatc atggccaaca atccgtttgt gaatttatcg ctaccccagt 85860

ttcggtggac attaattact tcagagcgcc atgcaaccca cgaggcaggg cttcgggatc 85920

tgcttacgca ggcgaaggag ataacgatat acgtgatgtg atgtatgacc acagccaggg 85980

cgatccgtca tatccatttc gggcgacaaa caatccatgg gcttctcaag aacaatcata 86040

tggcgatcgc ctttttaacg gaaaatataa tttaagcggc gcatcgcaaa tatacagtcc 86100

gtgttacaaa ttctttacac cggcagaggt agaaggaaag aggaaatgtt tactcaagat 86160

ggtgcttgaa gctggctctg cgttagctat gttccccggt gatacggaaa cgcagtttaa 86220

gcgaccacgc ggttctacag aactgaccga agatccgtgt ggccttttcc aggaagcgta 86280

tcctatacta tgttcgacag accaggcgct gctgcggacc gttggtacca gtgatattgg 86340

aggcgcggaa acgcatctgg ctcaatacct aattcgcgac gcatccccca tcaaaggctg 86400

tttgccaatg tgataaacac ccacaaaagt gaatggaacg cccccaaagt catgcacatg 86460

tttgtctact tcattaaccg gcggcgcgac gagagtatca gcactcatcg gcgaggtcgg 86520

taccatcaac atgacgacag aacagtttat agtggaaatc attattccat cgggtatatc 86580

gcatgcggac acatcggcac tgcaaaaatg tgaaggtaga attatatttc tttcgtccgt 86640

ccggcgtcgc atagcactca gtgatattga ctacaaatca ttttatacta atggaacaga 86700

accggacacg ctcgctttac tagcagcatt cagacgtcgc tttgcggctg ttattacccg 86760

tatcatgcct gggaaaatta ctgcggtggt acttggaatt ggcccgctta tctctggcct 86820

ttcgcttcag aatacatctc catttgacct aataaatgga gactctgtat gcttcttgcc 86880

cccaatgttg ggcggtggta ttggagatgg acgtctaaaa cttgattcta tcagtacaga 86940

acttatattt cctatgacta taccaggctc gatcgctcgg gaaatcatat ccaaagtagt 87000

ggctttgtcg ctaggggcgg tggtacaaac cgcccaaata caaccgaggg gtgttcagcg 87060

tgacaccata cctatacatt ataatggccg cagatataac ataactcctt caattcagaa 87120

cttggatgct gccgaaagcg ttgcaagaac gctcgtcttg aacatgatgt ttgcaatcag 87180

cgagggctgt atgatacttt tcgcactaat tcctcatctg ttgacgctca gcggacagga 87240

tggctacgtc aacgccttgg tccaactcca aagcgcagcg agggccgcat gccaattaat 87300

gaacatgcca aggcaaatac cacagataca ggatggcgac cgacgatttc caatatacga 87360

aaccatatca tggtggttaa acatgacaac cacactcaac gaaagtttgc gaccgcgtga 87420

gcaagtacga gtatgtgtta tagaaggacc gcctactatc aaacccggta atatggctcc 87480

tgtgatagat aattattaac attaaaatga ttatttttta tgcgtaacaa taaaaaagac 87540

atttccaagc aatacttgtc tgtcttgttt atttgttgta caaaaacata cctatggtac 87600

atatcctacg tgcgcaaaac aagtctcatt gttcgggaaa cacgccgcta gatacaatgc 87660

catgacaagg gccactagtg tatcatcagc acagccaccc gttttggctg taaatgtccg 87720

ctgctccgat ggcccggata tattttctga tagatttttc atttgtcgtg ttagatattc 87780

aactggatct gtacgtaaac tgatcgtagt agatactagt tcctgcgacg cgagcacatc 87840

ccctgagtta aatttgcgga taaataaatc aaaggcatca gttttttgtt tgtttaacag 87900

gaagaatgga tatgctacat ctccattggg aggcacgcag tggtaaaata atacagattt 87960

gggggctggg gcagtgcctg atttcgcaaa ggatgtaagt tctttgcaga tacacttagc 88020

tatagccacc gcagagtctt ggttactatt tccttccacg gctatacgta tagtaccaaa 88080

tctgcgttta tgtaaggcac agatttgtgc tatacatata gcagtacaca atcctattgc 88140

cgttgcagaa tctcccgtta gagcgcgtag aaaaaaatgt tccaacccgt atattacaaa 88200

gtcggtttta tatctaccta taacagcgat tcctgtcccc gatgctctag tattggccga 88260

gaaggccgga tctacataca caaatagatc ttgactcatt atttccatgt ttgcaacagt 88320

agacggtctg taaattaaaa atcgatcaag tgatgaataa gtaaatactt gcgcgtttgt 88380

tgacttttca ttacccgcta tctcaccccc gcctataatt tcttgcataa atgaatcggc 88440

cataaacatt tctgccgtac gtctcatagc cccatccata gttataaaga ctggtttgtt 88500

taggacgtaa catgagcacg acgtggcatt tgtatggttt gccactctgc gcatgtggtc 88560

atcacatata tacgtaacca cgttaagcaa ttcatcagtt gcgcctttga ggttataaag 88620

gaagctagtg ctactttttc cagtatttgt cgacgagaca aaaattatct tgcaattagt 88680

ctggttgaga aacccgacga tagtctgcac ggcatcggat ctgatgaagt tggcttcgtc 88740

tacgaacaac aggttaaaat cttgacctcg caaactctta gttgttcgtc gatagaagag 88800

aaaagaactg ttaatataga caggccatgg aatcgcatat aactaatgaa ttgatgatac 88860

tactaccgcg aagacgaaat gataaaacgt tatctttagt ccatatcatt ctgagcgatt 88920

cgtgccttac gagctgtggt ataaatatta gcgatttaaa aactacacta tcgcaaggac 88980

tacgcgcgca tctgactgta gaaactcaaa caagacgaca cgctactgga gaatgctcta 89040

attggaaagt aatatcagct gtctacgtgc cttcgaaagc tatacgtagt gttattatac 89100

cgtctgttcc taaacatcca actatttttc aatcctccga aagcgaagac agtgcagatg 89160

gggggctatt cgtctcttta ccgattgtat tagatcatga aggcagatat gatgccttcg 89220

acgttgtagc aatacgtata actatggata gagagaagta tgttgatata ttgtttacat 89280

atgatgaatt attaccacct ggcacaatgt atagaaagga tgaccggcgt ctcaggattc 89340

tttgtaagcg attatgtgcc tatattagta gtcagccgtg tttggaccca cgcctaattg 89400

aaataggtgc gcgcttggat gcatgtttat cacaggacac agactgccca atagatagag 89460

atgttgtcga ttcgtcaaaa cgcttgcagt ttgacggcgc atttgacagt ccacaaatag 89520

aatcgcggat aaatgaggac aaccgcgaaa ttatgtctct tatacgacgc gcggctgaag 89580

ttgttaataa gaaacgaccg atacgatctg ttaactccgc cgaacttaaa gttgcatctg 89640

gtttacggca aggcgtacgg gcatatttga gcgaatatgg gccatctggg actactggta 89700

gtaatagtgc tcaaagtcgt catagtacag caattgacca agcggccatg ttatcggggt 89760

tggaaccccc gggcgagact agatattctc gctcgcagac taattatcgg accaataatc 89820

attgtccatt agaggatgta cttatgctta ccgacaaaga caatgcacca ctttcatcct 89880

tagacttgtt cgatatgggg atgtgtgtga tttctggagg tgatacgcca gctgatctat 89940

ggaaacgtcg tgccatttct atcgtaccgc gcaggcatta tacatctgga aaaatttttg 90000

taatcgtttc ctatgctctt tctgaagcat ggggcgggca agcagacaaa gacagtatta 90060

atgccagtct gacccaagaa gtcatggctg cctgcgctag cgctggagta gattctccta 90120

ataaattgtg caaacgagct cgtgcggatc tagttgcgcg ttttcctgct ctggctggtt 90180

gtctattatc atcagatccg cctctcagac catttgatat tagcgcggaa acaatcttcg 90240

caaataaatt ccatactgct tgtataggtg ccattgcggc agcattgatg gaagtatgtc 90300

cgacaacttt acgccaagta ttacgttatg atatttcatg taaccagaga gactacatga 90360

aagttattgc agatacatca agtgatatga tcgaatccag tctaacagct tttaaaaatg 90420

agccattgga gttatttatg cagagcgctc tagcagaatc ggtactcgca tcactttgtt 90480

cgaaatccga gtggtcgtat caaagatact tcgagaatag ttccttgatt acatcgccat 90540

caaaaacgac gtttaaattc gactatttca gtgccggagg cggatgtata aaagtgtcta 90600

aaagacccat acccgtcata atcatcggca atactaatgg aatggaacag tgttcatgtg 90660

aattccaaaa taatttttct agtaaatatg tatgtgaacg gtatcttcca ggcgaatgtt 90720

atgcatacat atgttttggg tttaaccacc tcctagacat cattattgtt ttgccgggcg 90780

gttttacgct ttctactaat ttggccaagc attttaactg gccagatgac catatagatc 90840

ctattttaac tagattctgt aggagagttg atgtgtcctg acattcgtag gcggtacttt 90900

ctaacaataa agcgtgccac ccacagtcgc gtatatcata ttgttaagac ggcctgcggc 90960

ataatgacat ctaggaccgg acgcgataac tagatttttc gtcattcaag aattttgctt 91020

tctgcaacca tacgcccacg ctccagctaa accacctagg aattaatatg gctcgcagta 91080

ctattacacg tcgattatca tcatgtacgg aactcgacga tggagaactg aattcgccaa 91140

tattattttt aaatgacccg tctctcggta gtgttcacct agctctggca ttgaacacac 91200

aagtgtgttc atggcgcctt attagatctg attctcgtat caagatcatg atcgcaatta 91260

cagcactcgg tgaccgtctt tgtgctttcg cgcctccact agaagatcga gaaagggcgg 91320

caatggtgga aataatattg tacttaacgc gtcctaaagc gttagctctc ccatctggaa 91380

ctttccatgc cgtgtttatt gtcaaccgtt catcaatgta tgctgcaata gcagctatac 91440

atatcgaagc actaaaccaa tctggaaccc tgttctcatt attgttttcc tcagtagaaa 91500

cgaccccgcc gcctccggaa gttcctgacc cgtcgacaga aattatgccg caggcccctg 91560

cttcaatcct aaatctagaa gaccatacgg aaaatataac gccgccaagg gatcctcata 91620

actgtagaat ggtatctgtt ggggcgtggt ggtcttttcc caaacgaagg ctctactatt 91680

tacgaatgga tacaccactt ttagctatat gcccggcggg atggaaagca agaacgcttg 91740

gagacgttct agcgagactc gtagaccata caccaggttg cgagacctgc attagcggcc 91800

acgatcacgt ggattcgtat aatgccatat ggaagcctgg cgaagtcgca gaggcatgtt 91860

tatgtaaagg accatgcctg tggctcaaat caaaacagcg ggatatgata gtagaagggg 91920

atgtgagtat gtgtcgcgtt ttgttcatgg acgctgtaga tactatacga cttgtatcta 91980

accgtaatcc acgtatttct gcaaatttgg ccgaagtaat ttccgccttt ggttcagcca 92040

gacaagtacc tgtcaatgcg gccggatggc acttggtggc gttatcggaa attgctagtt 92100

cgatcatgat atctggttgc gcgcgtctta gacgtctatg ttatcccaaa acataatata 92160

ctgtctaaaa actattgctg tatggttatt aaataaaagc attactcaag actcaaggct 92220

tggcaattta actatatacc gcgcatgatg ttaatactta catttgtatt atggctcgac 92280

gcaaatgtta cggtgctctt tgaaccatct tgaaacgaaa aagatatagt ctctcccttt 92340

atatgctcta cccttccatt tccgaaccaa cgccgtattc tagcatcaat ttcatcgaat 92400

actggctctg tagccttccg tatatgtgca gtatagccta tctttattcc tctaaatttg 92460

gtaagagcta acgctattag tggcactata aaccatgtct tcccatggcg acgtggtacc 92520

agaaatactg tggcgcgttg gcgaaaatgt ctcagcgatg tttcagaaaa taatggtata 92580

tcaaagacga tgcgcagaaa attatcagcc ttatcggcat gatcaccaag tattacagat 92640

gcgaggaaat aggtcgcatg cattaaaatc atcttctgga ataattcaag agtaccgcgc 92700

atctttccat aagttggcac atcgacctta cttctctttg cctttatagt atttgcatcc 92760

gtcccagcat cttggaaaga tgtatccaat aaacgagcga aactatttat aaaaccattg 92820

agttgcccaa attcatccga atcgcgcata gctttaaatg catttaacac actatagtat 92880

gcattcttat gtgctatcgt ttcttcctct ccatattctg caaatcttaa cgattcaaac 92940

gtttttatca tatccggcac aaggaatgct tctcgtaccg gtcttttaaa cgacgccgtt 93000

tctcccgact ctgaggaact gtcagaaagg agactagaga aacgtaaata tctggcgacg 93060

ccagaaaata ttggtgcatg ctcgcaacag tcatgaagcg ttccaatggc cggaattaca 93120

gtttgatgcc gtcgaggtac acaggtggca aagtttaaaa acggaacggc gatatcacta 93180

tcttcgagat ttgccgtcga agaacttgta tcgccagacc catttctttc ttcgtatttg 93240

cggcgcaatt cttcaaaata ttttctactc tctctgccga aagttgcccc gaacattatt 93300

tgcaatgtat gtaaaaattc ttcacataat acgccactga tcaattagta aaaatgtcat 93360

gcgtttctga ctaagtagta gggcggtaca acctgataaa ttcaagcgtt ttacactcct 93420

cggcacaatt tagattggcg aaaggctgca atacggacta ttttatacag caagtgtaag 93480

tagatatatg gggtataaca caaacatagt cgcatgggtg tgggtaagga ggggtttaag 93540

gcaaagaaat atgatttgtt ttttcctact ctattgaata gtgcgcactc tcgctaacat 93600

gctatcgcgg cttcaagatc gtcaaagacg tctcagattg gccgaggccc ataaacgcga 93660

agaggtttac aaagctcgcg ctttggactt gttgcgggta ggcgtggata aaaatagtgc 93720

cgtgttcgtt gacactttta cgtcagcgaa agcagcccac gtggcgtact gcgcccaatt 93780

gcgctcaaat atgcgcgttg aacaagtaaa gcaaaaggtg agaactgttc agacgaagat 93840

agaagaacaa ctcgcaataa aggatgcgct agaggcgcac agaaggtttt tatcgccagg 93900

cctaattgat cgtctagatg atgaagaaga taaactcgca ataaaggagg atttattaac 93960

tgaagttgca gaaaggtgtc taccgggacc cgaaaacact atagggggac tatctggtaa 94020

cgaatggctg gacgaagacg acgaagccct attagcgaaa tgaatcctat cggaagctcc 94080

gccaagaaac aacgatcttc ctcaaagccc aacacaagcg tatatgcgtt tgcctgggtc 94140

ccagacagca ctgaaaattc aatcgaaatg cgcgactgta gcgaaagccc caatatcacc 94200

tcagcaggaa aacgacgatg tagcggaata cgccgacttg gaggagcgac tgtgtattct 94260

ggagaacata acagtaaaga gagaaaggat cctggcaaac gtaagacaaa gagtagatgg 94320

agcctggctt cgcggttctc gctcgtctcg cctcccgggg ggaagccact aacacgcgca 94380

cctagaatgc catcttatat tctgcaagct acaaggatag aaaatgtatt caatgtagta 94440

ttctctataa atccctcgtt gcattttact tctgtcgcac taccagaaca gccttctttc 94500

ctcggatccg gtggctacgg agaggttcag ttatacaaac gcaatggtat cgccttaaag 94560

acgtctagac aacatttcag aggtgaattg cttatgacat tattggctag cgaaagtggt 94620

ttgagggcca aaagtatcat tggggaagat cgtattatta ctatcttagg tttttcacta 94680

ccatacaagc aaatgatatt caaagcatac gatatggata tgaccttata ttcacataaa 94740

ctagcaaaaa ttactccgaa agcccgtcat tgggctgcgt tagaacgcgc atatattggg 94800

ctggcaaagg cactaacatt tcttaacatc agctgcggat taacacacct tgacgtgaaa 94860

agcgaaaaca ttttcataaa cgttgtcagt ggaacggacc tagttctatt agatgcagta 94920

ctaggcgatt ttagtctcat gttactaaat gctaattcaa ctattatgag atctgaattg 94980

gaaattcaaa caggagccac caaccctacc aagatcagga tttatcgtcg taatgtgcag 95040

cctatttttt gtatggtcct ggggcatgca tatgcccaac cgccagaaat tttactaaat 95100

gcactaaatg gtgatgggct gacgacacca atagaaccaa ttaatccgga ggaggggctg 95160

gcaattgaca tatacgccct tggccaatca ctgattgagg tgttgattaa cgcatcttta 95220

aatcctctaa acagacttgc gttagtccga aatccaactt acttttattt tcatcatcaa 95280

gtgaggagag attttcttct ctctagtttg gcgtaccgtt gcatcttctt tcgtcgtata 95340

tttcctacga cagacctaac gatccaattt ggtaatccat ggaataatta tgacgacata 95400

cggcacaaat taagagattt ggaataccgt ggttcatttg gagcacatta caatcgattc 95460

aatttgacgc acggaagact gtttgatgaa ctagtgattc ctcaaagtat atcacagtta 95520

ctggaacttg cagctctttt ttgtcacgct aacccaaagg cgagaagcgc cgctcctcta 95580

ttgtggattt ataactaact accatgagtg gggatataga tggtagctca tcgccagtac 95640

atcactgcaa acgtcgtcga aacacttcat ggaatagttc tcccgagaat attggcaacg 95700

cggacgaaac gtacggtaat tgtgattata caacacttgg agaatctaac gaacatacta 95760

gtaataatga attgtgctct attgaacatg actggggtta cgttggacgg tttggaccat 95820

tgccggactc actaccacaa tgtattcatg agctcaattt tcatgactat ctagagtcag 95880

aactgaaacg ttctgactta gcgttagatg taccaccact gtatcaccgc ttagcatttt 95940

ttcgcgattt aattttaaag ttggaagaga ttggtctggt gtttggagca gcacctaaac 96000

tatttggagg ggcgcaagtt tgccgtaaac ctgcagaggt attgatgaat gcaataacaa 96060

aagatcgtgc catgaacatt gcattgactt ttgaagctga gacgcgcaac caagccaatt 96120

gtgatctttg gaaaatttta agacgctgta tgatgaccgc gtcgtctatg aaatggaaca 96180

aatatgggcc gtactatgag cctgaatggc aactagaaga tacgaagatg caggaaatgt 96240

tatctgtgcg tagcatacta ttcggaaata ccaatgaacc gctagtccgt gcaatgatta 96300

tggcctatcg tttaggtcca agcattccta aaacccccga tgggttagac ccgagtcatg 96360

atggtgtatt tgtatttgac gacgaaaagt ctccagagcc atatacatgt ggcttattgt 96420

tagatcatcg tacaggaatg atgggagcgt ctatggatat gcttatttgc gatagagatt 96480

cgtctgggaa gttagcgccg catcatacgg aaaaaactct ggagatatac gagataaaat 96540

gccgcgccaa atacatcttc tgcgctacgg atataacaaa taaaaccgcc cgtatgtaca 96600

atgctcttat gaaagataga tctttcaaag catttaagcg attcattaat tcaataagca 96660

agccgtgcat cgaatatttt gatccaaaac gcctaccaag tgcagccgaa gcgctagtga 96720

cgtgtgatga atcatggaag aggcgtgatg acacggcaaa aagagaacga tgcttccata 96780

ttgatagaca ccacctgtta ttaaataagg cgggtcggtc atcagttctg ttatttagcg 96840

aaccgaatct agatactcga acagtctgcc aggccccctg ggcggacggc tcgctgatac 96900

tggagacgcc agtatttgct aaccccaaac attccaactt taaacagatt ttcgtacaac 96960

actatgtatt atctggctac tttccggaac ataaaataca gccgtttctc gtaacattct 97020

ttggccgtgt tcgcaagcca gaagaagtcg gtattcgctt cgaactatct gggacattgg 97080

gagtattacc ttacgccggc tttgacttat ctatatcacc agaacaggcg ataccagtcg 97140

cgctcatctt tacgccagtt gaagtagata ctgctctatt tgaagtaata gaaaaagctg 97200

gtttgagagc cttcgatacg acagctacac aactatggga caagctcaat cctacgggag 97260

cggtggatgg tgctgcagaa gaaacatatt aactactagt aatggcgatg ttttagacct 97320

tactgccgac agctttgaag atttcgaact tctcggtcca accgacaatg acaactttga 97380

gacgagtaag accatcgtaa cgcaacctca aagaaacacc aaaaagggca agaatgacta 97440

cagcggtaga aaagataggc gttcttgtta tagaaacccc aaaaatgatg ctttgtaaca 97500

aataattaat aaacgctact ttcaatgaat gggtgttgtg taatcattaa aacgttaatg 97560

aatatattgc taatagaata aacgggactc aataaacttt ctaaaacttt tattattcaa 97620

accattctat agttattatt ctcattcact atccgacgag aaatgagtct cagctacagt 97680

atcatatatt ggctcttcct cctcatcttc tacatggttc tttgaagcac gcggtccgcg 97740

tccttgtctt ctggatccat acatcttcac tttatcccgc acgtctcgta cgcgtccata 97800

aaaacgcgta acgtttcgcc tatggtatat atatgctcta aatatacgta caagcatcat 97860

aatcaagatt atggtgctta gtattccgag cgaaagtctt acgaataagg ttattccctg 97920

tacattttct cccaaggcat aataaaacct gttcagatag tcgtgggacg atgtgccaat 97980

catcccacac cccgctaaaa gaccaatatg ggcgccgata attacatgca cgtagcgcgc 98040

aactacaaac tcgacggcca ggaggaagaa aatagttatt gcgataaaaa cggaaattcc 98100

gactgttata ggttgccata gttgcataga aaaactgttt gccattaaca tttccaacac 98160

aatagccatt atgatgatcg acaatccgta aagcgccgag ataatgttta taatcacagc 98220

ccttacattt ccggctatta tatgcatccg cgaactgagc gatcgtagtg ttcttacgtc 98280

gtcggcatat gtcacactat ttactcctct tccacagaag tagatataga agaatgtaaa 98340

taaaataaaa tgtaccaaat aagtacatgc cgcgagtaca attagcttat gggaaagcaa 98400

tactactaca gtctgtaggg tccacattga taagcttcca actaggatag ttgccggcgg 98460

tactataagc gatgctagat gagcgtggtt ggcgcatgct gacgtttttg cgtctcgttt 98520

caatattatt ccgcctgtga tcatataaca tgccgttgcg atgagtacca gtccaaacga 98580

atagaaaaaa aataacacct caccttgctc aaaaaatagg gcgggtacaa ttcctcccat 98640

aattggattg gtgaatccgc cctctattgt cttattaaat gttgtataat ctacaacggc 98700

ggcaaaaaaa caagggaatc cggttgtctt gaatgacgcg gagatcagcg tgactgtata 98760

catcaacaag actaacacaa aacaggctac ttgcaagaac cataggcgta gatgaacgca 98820

atcaattcgc cccgcaggat ttaaacgata cggcccattc atggatccca ttgttaccga 98880

tggcgtcgat cccggttcct gttaccaaga tgatagaagg gcaacagatg accacacgtg 98940

cagtgcgtcc cttgctaaaa tgctgtatgg cggagacctt attgagtgga tttcgcgcta 99000

caggcccggc gttacctccg aagtgcaatc gacgggaccg gtatccttcc ccaagccaga 99060

tgataaaaga agccgtcgag tgacggtagt acgcgcgcct atgggttctg gtaagacaac 99120

tgcgctgata gaatggttac gagaaatact tcatagccca gacatcagcg ctctggtagt 99180

atcgtgcagg cgcagtttca ctcatacgtt agcaaggcga tttaacgacg ctggattggt 99240

cggttttgtc acgtatttta cgtctagtga ttacatcatg cgcggtaaga cgtttacccg 99300

tttgatagta cagatagaat ccctccgccg cgtggagctt tccctagtcg acaactatga 99360

cgttctcata ttagatgaag ttatgtccac attcgcgcaa ctgtattccc ctaccatgcg 99420

atatttacac caagtagacg aattaatgac acgcttaata cgccactgcc cgcgaattgt 99480

tgctatggat gcgacagtca atgcccaatt agtagagtta ttggctgatg ttaggggaga 99540

tagtaacgtt catgtaatcg tcggagaata tacctccgcc gggttcgcgg accgcgcatg 99600

catgattatg cgcgatatgg gggtagatac gctgcgcgct gcgctaaacc cgccttcgga 99660

aaccacagcg acgcaaaata tgcaaagcga aaacgacgat ttggacgccc agcaggtcca 99720

acagccacta agaccggata cgtttttcgc cgaattgggc agaagattat ctgggggtct 99780

taatatatgt attttttctt caacggtatc attctctgaa atcgttgcta aattctgcct 99840

taaatataca gactctatca tagtgttgaa ctctacaaga ccccagcaat cagatgttac 99900

gacatggaat caatatagag tactgatata cactactgta gtgactgtcg gactgagttt 99960

cgatgaatca catttccaca gcatgttcgc atacgtaaaa ccaatgatac atggtcctga 100020

tatggccact gtttatcaat ctctgggccg tattcgcctc ctacgattga acgaggtgtt 100080

gatgtatatt gacggctctg gtgcgcgctc ggacccaata tttacatcaa tgttactaaa 100140

tcatgttgtg gcgactggcg gtggatggcc tactaaattc tcccaagtaa ctaacctcct 100200

atgctgtaga tttcgtgata gatgtgcgcc tacgttttcc gaatcatcgc gcgatttatt 100260

tttgttttca cgtcttaagt acaaacactt ttttgaacga tgtactctca acagtcttaa 100320

cgatagtcta aatataatgt atgctctcct agaatccaat cgcatacgca ttaatcttgg 100380

agggcatgaa ccatcagcag atgaattttg tcgcctacta tcagacatac aacgcaactc 100440

cataacaact cagcgacaat tacgtgcatt gaaaaaacat ggaaatggac gcctaccacc 100500

ggcggtagag gttgccgaca gtgaagaggc ggccattttc tcagaaaaat atctccgacc 100560

agatgcatca atagtagata ctacagagct actcaaagca atggccagcc caattgtacg 100620

cgaacatttt gtcaacactg ttatgattga agcatgtcgt agaatacccg ccgcgatgtt 100680

aagcgaaacg gtttttagac gcttgtattg tcattacggt tctggcacag tacctaccat 100740

aacaaacgat ggaacgattg aaacggtagc tcttgtatgc gattttaatt ggccagccag 100800

atgggactta tatagactat gtgcaaagat ggcagatgcg attaaattag acctgcgcaa 100860

tggaggcgct accgtcgacc taaacttaga tattatatct gatacattgc gccctgatta 100920

tagcaactat attagattac tattggaagt ctcaagatgt aatattacag aatatgactt 100980

agttgcaccc cagccagtac gtgaggtaca agctgcgctg gccggaatgc ccaataacat 101040

caggcaagtg gggcctgatg tacatgcaat gttattattt aaagtaattt tatatgaaat 101100

gttaggtgca catctaatgc gtagtacgcg cagttttccc ggatcagtaa aggttaaaaa 101160

tcttaaaaag gaggaaattg tgacgttatt gacagaggcg ggtattgact tttctgactg 101220

ccgcacacat aaagatctgt acgcacgcct aatgagccac aaaaacaaat ttaaaggtac 101280

aagatatcgc attaaatcac ctaaatggac taaattattg cgcagtgcca gagatttgga 101340

caatgatgga tgggagccga gtctagaaac tgtattggct gacataccat cggaacattg 101400

gccgagggcc gagggagcag ttgattacag acaattataa tttgaggcct aaacactagg 101460

tatggaactg caacgcgatg gaggatttat aggcactaag gaacaattta ctagtattga 101520

tggatatatt tgctcgacct caatatatac tctgtggact cgcccaggaa gtaaaaatgt 101580

actgtccgta ctatcgtata tactattcag gaacattaaa aactctcgat acgaagccca 101640

ctttaccacg gttgatatta atctcaatgc aatggaagag accagcttgg cggaagaagc 101700

ggccctaaca acaacatggg caatgacggc atgtcgggat acttcacgac caggggcatg 101760

gcctatgtct agtatggcga acgtcggaaa ctggaaacgc cttagctgtt tggtatctgc 101820

gcatgtacgt acgaaattac gctatatgtc attttattcc cccgtgcgcc ttaaagtaga 101880

cagtaacact ggcctggtta tatataccga gccgataaac ttaacttcgg tacctatcga 101940

gcccaaagtt ggcattctaa cagccactgc ctgcttgcca attgataatg cagcagttgt 102000

agcccaaatt cgaagttctg ctggaccatc actggctaag gctaggatgg ccgcagcgtt 102060

caaacttgac atgaccaaat cacatcatcg tccaaatgac catatatatt cgttagaagt 102120

atctatctcc actaaaaacg ctaaatactc aagacgctat tccgatgatg tttatgcccc 102180

acctgccaaa agttcatgct caggggatag catgtttgag ataattgacc gtacgctttt 102240

tttggatggg cgcgaaataa gtatacgtgt gttgttgcct aacggatttg actgtctcgt 102300

cgcttgtgcc gacggatttt ctgcattgtc cgtgctggct gtatatagac actggcatga 102360

aacgatttac gatatgcgca aatcctgcgc gatcgttccg atctttgcat atgtgggacc 102420

tgaactttat ggccgcggtg aggaacacga ctactttttc accgtcggct tccctggctg 102480

gccaacgtta aaggccaagg cttctgaaga tggcatcaaa gaaacggtca gtacatatgt 102540

cgatttagat ggtctgtggc caatgtcagg cggcatggca ttgcacttat tggcaccatg 102600

ggcccctgaa aaacgaccag gtaatgattt cggaacagat gtcgtggacg ttctagctaa 102660

tgcaaagatg aatgctctgc gagaaaaatg gcctgccggt cgaatgacat gcgttctacc 102720

ccacgggaca tttatcgatg gtgctcatgt ggtccagatg gacttttctg cctttacgcc 102780

ctcgctatat ctgacattat atccatcgca taaacgactg gagcacgcgg ttagagcaag 102840

acataaacgc gaaaaaccat ggctaaagaa agcattagta gagttctgtg gcatgttgaa 102900

acatactcat ccagaagcac tcgatgcaac gatagcgatt tcaaatgcca taagtgaagc 102960

tatagaagaa actgctacgg caaatgggtt agctatctgt acctatataa aagatggatt 103020

ttggggggta ttacctaaag agaataatca gcgcccaaac gatgacattg tgaatatatg 103080

cgctctgtgt cagaaggcgg cggaaaacgt actacatgac ctacatctgt tgagacccac 103140

cggccccggt ataaaactaa gggtcgaaga ggggacatat acacatgcta tgtcgttggg 103200

tatgcatcat tactggttgg ggggcgtcga cagtgaagat tacaaactcg tcggctttcc 103260

aatacgtaac acatttagtg aaaacgtagc caaaacagta gcaacgttac taagttgcgt 103320

agccgctaac tccaactacc gtatacttta tcctcttttt aaagaggcat ttgatcgact 103380

aataacaacg gcctttgaaa atagaaacga cagtacattt tggagcgtag atgtaccgat 103440

atcggatgag ccaataccat caggtgcatt tagttacaat gatcatatgg atatggatca 103500

tgggaggaga cgtgtagttg aagtaaagac ttttagtgag cccgtaacca ttccatgttc 103560

attgctcccg ggaccaatga tagttcccgg aataatgtgc ctaaagtaca tacgaccggc 103620

attaaattct gcttgttcca tattatcgca cgccgtttca tctaaatggt gcaatgaaga 103680

tgtagaagcg tttacttatg atgttgaaca attcgaaccg ctatttgctt aactactaac 103740

tacctcacac taatatttga aataaaacgt ttgaagacac aaatgaataa attgtatttt 103800

ttatttgttt cataaacaac taagttagta tgtataataa agctgttctc ccgtaagctt 103860

ctttttctga ctgatccacc aatcatagat agcgtctctt atccactcac atttcagcgc 103920

ttcgttgagc actccgtcag ctatcacgat tgcatcagat gggcattttt gtacatccga 103980

tggtaaatca aacttcttcc cacccttggc cgtatatatg gctgcgagta cagcgggcgg 104040

ctcgtccaat atagacaatt gtcttattat tgcatgatta ggtagtacat gtttggggtc 104100

aaatggttca cgaccactta gcagcatgag cgtattcagt agccatgttg caccaatgca 104160

aagtagtctg cgcatctttg tcataaccgt tactgtagac catgccttcc taataaaagt 104220

atgcagttgt acaaataatg acgaacatat gcgctctctg ggacaattct ccaaaaacat 104280

aataaccaga cataacgcaa agtccaagtg ttcggaaggc ttaaatatag tcatacgatg 104340

cttcaaaatt attggcggga cagccaatgt ctgaacgcga tcttcggcag tcgtgataac 104400

agcaaatgca aaccctctaa atgcagtctg agcgagacat tgagtgtgat atacattact 104460

aggtacttcg atggaattag acgagcctgt cagttgcaaa tataactgtt gtaggttatt 104520

actatttacc ctcagactca tgactgaggc gcctgtaaac cgcgtaggga tgcctggtac 104580

ctctcgtact tcgcacatta gacgcggtat cgcctgccaa cttatatcgt cgacgagtgt 104640

cccgagcttt tcgttcccac tcgctgcgag cttgacgagc tcgtttaacg ttgcgctgtc 104700

ctccagagga tggtttgtcc cgtaattcat aatcttccga accgcttgag taccgccgtc 104760

catgatacat gcgctctggt tcatatccat cttccgcaaa tcctttgcgt tttacggaaa 104820

ccgcgccgct ttcgaacaat gcctcttcgc gtgcaatgtt cgatatcgca atttccctct 104880

tgacatcggc tacgaatagc gcgaagaggt ctgctaaata tgtttcgagg cgggtctttt 104940

ttaatattga aacacatagc tcctcctccg aacgatatgc ttcctgattt gttaccacaa 105000

accccaagcg ccgtacacgc aatacagaga aaaaatatgg tgcaacgagg agagttattg 105060

cgctattaga gtatgacagt gctacctctt gaccctgatt atttgtagta cgattcatct 105120

tgaaacaacg cactagttcc tgttcccaca aactagataa tcttcgcaat tcatctgcat 105180

aacttggtat gtaacgcgat tgaaaactgt tggcaacata tgcatcatcg cccatacgtc 105240

cactaagatc tattacctca tgtcccaaat ctctaatgaa tgttgtgatt gttccaggag 105300

tgtcagacat aaccggcaat cctcgcggca tagtttctat gccgccaatt tgtaaagaat 105360

taccagaaga attagtagct gagtctatag cagccattcg cgcctttgct aaattctctt 105420

cacttgtgcg caggcgtgag gccagttctc catttgtctc tttgagacct tcaattgttt 105480

taaatagatt atttacataa ctctctaaca gaccattaat actgttgatt acggaagttc 105540

taaatgcctc atgcacccta acggctcctg tagtttcaaa ctgtccactg gcatttgctt 105600

gatttccgcg accgaaagcc ttcccaaaac cggcctgaga cgtatctaca gacgtatcgt 105660

ctaacaaatg ccctgcagtt tcatctaaat acgctctaac tgtatcggta atatctccaa 105720

cgtgtctcat gccctttagg ttaataatta attttactag cctagacgcc gccgatccgg 105780

ccgcggcatc ttcttgagcg cctaaagctt tttcgactgc cttttgggca ccgacaattc 105840

cacgttcttc ttccggtttc ctaccaagta atacctttac tggagcagta tttaacaact 105900

gacatacccg agcatgttct ctcaaatcgt ggcacatcat cacttctcta tggaggcgct 105960

ggagaggaga atcaaatatt acagtaccat ccttccatag tggttgccac accaccatac 106020

actccccgcg ctggcctgtc acagaatcct tgacaaatac ctcacgtttt ttaaaattgt 106080

aaaagatgat ggccctatca taatcgagta ttgtaacggc atcgacttta tttgcaagtt 106140

cggctgctac ttgcctacca cgctctaata cgctatcagc aactaacaaa cgatcgcata 106200

gctctgtcgt taccacatta tccgaaccga catcttcatt caagcagccg gccgtatcgt 106260

gtaaatgccg tctaaaggaa cttgatttac agtgttcgtt tcttattagt ggtaccatac 106320

ccagacaaca tatccaatct atatatttag aaaagctggc ggtaccatcc ggttcggtgg 106380

acgaaaaaca cgcgtttacg ccacgaatca aatctataag ggctgtttgg agtgtttgtt 106440

tccaggtact aaatatctgt tcggctaccc gcacggcttc agcttcacta tattctccat 106500

atctcttagc tatatgtcgt gcatctaacc ccctagtttt tacatgcgtt tcccaatcgc 106560

ccctaagatc ttcataacgc acagcgtcta atgtgttggc tagtatagtc gcctggattt 106620

gtcgtatggc agcttcggtc gaccttaccg aattataaac tccctggcca tctgtatatc 106680

ccaattcgcc gagtaaaatt ttcttgaata aacaagttct ttccgtagga tgtatccgta 106740

cccaatcgca aattccatca catatatcat gcagcccaca ttgtaactta tctgttatca 106800

tgctttccga gtgttgttgc cttccgccgg tattgatacg tctttgtttg ctcctcccag 106860

taccccagtc gtgctgggcg ggacatatcc cgcattggtt agctgacata ttaatgctca 106920

aatcagcagt agattatgaa ccaaacaggt tacccttggt tcatcatatt catctgcgta 106980

aaaaaaagag aggctattat ggaaatgcaa ttaacgacac aatccgttga ttatccagag 107040

acggtatatt taaattttac ctctatgcat ggaatcagac caatagtaga tcgtattcgt 107100

tctatggcct cttcgcgcgt acctgctacg aaaatacctc cgctgtcttg gttccagaag 107160

gctttaacac tacaaacgcc gctggacttg gaactgagag aacttccgtt ctactcatac 107220

ctcatcagcg gtaacgccgg atctgggaaa agcacgtgca tacaaacgct taatgaaatt 107280

attgactgcg tcataaccgg gaccactcga gtagcatccc agaacgtcta cgcaaagctg 107340

tcggcggcat actcttcgcg ccatattaat acaattttcc aggaatttgg attccgcggt 107400

aaccatgtac aagcgcaact tggcaagtat caatattcat gtccatcaac accacctact 107460

atacaggagc tccaaaagag agatatagtt tattattggg acgtcctaat tgacatcact 107520

cgtaatattg ccgatggtgt agacagaaaa caggggggaa gtaccttcga ccttataaga 107580

tctattgaag acgctctatg ctatcctaga ggatcattag gagaccttgc ctattgtatt 107640

aatggatctt tgccatcgtt tacacgatct aacgtaatca tcattgatga agctggtctc 107700

ttgggtcgcc accttcttac tgccgtgata tactgttggt ggctcatcaa tgcctcatac 107760

ggtactcagc aatatatgga tggtaaggct ccagtcttag tttgtgtcgg ttcccctaca 107820

caaaccgcct ccctggaatc tacttttgaa cacgccaact tgaaatgcaa agtacggtca 107880

agtgaaaata tattgacttt ccttatatgc aatcgtacaa tgcgaactta tacgaatcta 107940

cctaataact ggtcaatatt cataaacaac aaacgctgtc gcgaacaggc atttggagat 108000

ttgctaaagg ttttagaata tggtctacct attacggaag agcatgcgcg cctagtagat 108060

aattttgtag tgccggaggc ttacatcaac aaccctgcaa atttgcctgg ctggactagg 108120

ttatattcct cccatcgcga agtcagcgct tatatgacgc gcttacatgc acaccttaaa 108180

gtatccgggg aagacaaatt cgttgttttc acactgccat catatacgtt cgttaacatc 108240

gaaaacttcg aaacataccg caaaactgcc aatcagccaa atctatcagt tgagcgctgg 108300

ataacagcca atgctggaag attgagtaat tggtctcaaa gtcgcgatca agatactagc 108360

caacttagat gtgaggtgta tcgtgacaaa ggattaatac tcgttagttc tgatataact 108420

tttgtcatga acagtcaagt agcagtaacc acccgactaa agagatggat atttgggttt 108480

aatggtacat ttgaatcatt ctggaacgta cttaaagatg atatgtttac aaaaacgcaa 108540

ggagaaacat ccatagagta tgcacataga tttctgtcat cattgatgta tagtggactt 108600

atcaattttt ataactttct acaacagaga ggcttaaaga cagactctgt cacaacggca 108660

tacagacgca tggccgagct tactaccact actatcggta gtacgtgtac cgagacatcc 108720

cagacgggcg ctggcaatac caacgcattc gatcatgaga tgtatcgttt tgatgactcc 108780

gcggcgcaac aaaaacaatc tactgcagat gatgatgtag tgttcggagc attgtgcgat 108840

gatatgatcg acatgctata ctgcaactat gatttccgtc ggccagaaac aacggctgag 108900

gtgtatgcac agtttgcaat actcaagaca cttttcatgg gccgatatgc cattttgaaa 108960

gagttatttg gccaacaatt ttgcaaatcc aatttctgta catacgtaaa taatgttaac 109020

ttccgcggct gtgaaatgtt tacttcgtct ctccgcggag gtatgatgtc catggctcta 109080

cagacagaca cctatacatt ggctggttac acacgcgtaa ctattcaagc attcacggac 109140

gatcctatac gtcgttatgt aagcgatcaa actaacgagc tgttacatga aattggcatg 109200

ccgttaatgg tattgcaaga tcaccaagga tttatgtccg tactgaacct gaacatatgc 109260

gattttgtag accccgttgg agataaggat ctaaatatgg ccactgtggt agattatggc 109320

ctcagttcta aattggccat gactatagcg cgctcacagg gtcttggcct agaaaaagtt 109380

gctatctgtt tttcaaagaa tggacttaga cttaatagtg tgtacgttgc aatgtctagg 109440

gtagtaacgt ctgaattttt aagaatgaac ataaatccac tacgagaaga atgtacaaga 109500

gaagatgata tcagcgaaca tatactcgca gcattgagag acccaaacgt gcacatagtt 109560

tattgacgaa ttataagaac tgctggtacc ttgcccacgc tcgcgcccat tatacatgcg 109620

atcgcatatg caagatatca tcaggtctta taactatacc tctggtagtg cgcatcagtg 109680

tattgtgtct gctgtcttgt cgtttccttt taataaatta ctgatggaat atggcgcgcc 109740

gacgttcatc cgttggtgtt tagcgttatc cgcccactgt gtaaacatca acggacaaaa 109800

cccggcgcga catccgcacc tgctcatcga cgtggcgcca gcattcacag acggccatgc 109860

aatcgcatcc ggcaacgttt ataacttaca ctctgggggg taccggtgct tcgcatacgt 109920

ggactgttcc agaatatgaa caagtgatct gttcgtgtga tggaggatcg agatctgttc 109980

tggtcgggaa caagacacgc tgtgacaaac tccctccgtg taatgttatt attcaacgcg 110040

gccctcttgg gactctattc gtcgtagata ttgggtatgc aatatattcc tatatgctac 110100

gttgtgatct aaaacaacag gtcggtacat tatcagcctc acctggttca ttatatgtag 110160

ttccgtttac atcatgtacc gtagtcggag tagatagcta catccgcagt gactctagtg 110220

gtgtattaac gattgcatgg tctcataata cagtgcatat aacaataact gtatatggtc 110280

tgtcggaaga gtctcagcgc atggcaagcg tttcggccat atctactgtc gggcaagact 110340

atgaaaatct tcaggatata gccaaccaag agcaaagtga agatttacta tctgctgcaa 110400

taaaagaagc taatattggt gtcgactaca tatcagatag tgagtcgtca tctagaacgg 110460

ttatggacga cttactaact tctattcaag atgaatgcct agagacggcc gactgcttct 110520

gcaatggaac gtctcaggag ccaattgcag tgaacggtat agacatttaa cctttaggtc 110580

tgtattttat ttccccctcc cccgaggacg taaataaata aaacagtaca caaaagattt 110640

ttatattgtt tgtttattat tttgtgaata ctcccattta cccactaaga ttctaaacgc 110700

gattgctggt ctatgagtcg ataacggcgg tgttgatgat tacctggttc aggccgctcg 110760

tttccgccga gtgatccatt aatgagaatg aaccttatga tctttcaaag gaattgacgg 110820

cagtccatct ttttgtttag cgtagttttt acttcgcccg ttaatgcaaa gctctccaac 110880

agtaaacccg cttcgtctgc gttgtctctt agaggcgtca atagagacgg gatgagacgg 110940

cgccggttct gaaccgcgac gtctgcgctc gcaactttcg aaccctaccc ctgatgcttt 111000

ttcccaacca ccacatagcg caaaatcacc tgtccgctga cgtctgctgg cggacttgtg 111060

tagtttgcgt gcagcttgag gcatggcaga cacaaaacgc ctaaagtcat cactagatgc 111120

taatgccgcg cgcccgaaca tagttcgcga tcacttcagg actgaggcgt actgacctct 111180

attacgttgt cgcgatgtaa aaagacagga tctacggcag caaactcgta tacatagact 111240

ggaactacgg gttgggtacg gccgtctgca gaacgcgtat agtacttcct cggcttccta 111300

gcctgaataa cagtctgtaa ccgatcgcgt atatacttcg cgtgtactct gcgacacaac 111360

acaaacatct gcaggctttt attgccgtgg ccacccttta ctgtttgtct tgacttactc 111420

cgcctgttag aggatgacga aaacgacgaa gtcttagtac atgctattgt aaatttggct 111480

aaagaatgtc gcaagtcctt gcgaatggta tcggtgagaa ggcgtctccc gagctcgtca 111540

atcgacgaaa ccataaacat agtatcaaaa cttacatagt tttgatcaca tgttgtttca 111600

ttcggggacg taccgcaccg tcctcgttcg tcgtaagtac ccgcaggtgc cccattctgt 111660

atattaacat cagatgttag cacttccgcg cccggaccgc ggtcgcgttg gcaatcaaac 111720

ttacttctac tctgttgccc gttcgccgct cccatagacc acccactgca taatgttccc 111780

ctttgatcga tgcgtgtgca atcacgttgc gttgtactac attcccaacc ccaatcacca 111840

agtaccgtta taatagatgg tactggggtg tccatagtcg aggactaagt ccaaccagac 111900

gggtgagtcg cgtgcccttt ttataaaggt gtaagttaca tccgtaacgt cgtttatact 111960

gttccacaag gaagttgcca gtcaaccggc tcaacgccac gaccttccag gtattcattg 112020

gcctttttaa aatgatcaca taagatgaat ggctttctag acagcggtga tggatggcta 112080

tatttcaaaa cacaatgctt gcgacaattc ggtttgaacg cttcctgagc atgcacgccc 112140

cacagcataa acactaatcc ttccttattg tcatgtaacc attgtaatac agatttgact 112200

atttttgacc aaccaacgtc tacatgcgat cccggatgcc cctttcgtac tgtgagggtg 112260

gtatttaaca acaaaactcc agctttggcc catgcctcta ggcagccatg atcaacaatt 112320

ttcgtctctg ggtaagagcg ccgaacggcc gataatatat tacgtaggct aggaggtatc 112380

tgaatacctc tccgcacgct gaatgcgagc ccgtgagcct ggccgggttg atgatatgga 112440

tcttgcccaa ctatgatgac ttttacatcc gatggagcac agtacctcgt ccatgtaaat 112500

atttcatttt ttggcggcag gacttcttcg atgtcacagc gccgttcata ctcaagtaat 112560

actcgagacc ccacttccga ttgtagctct tggtataata cttcccgcca agcatcgcca 112620

atacaataat cgccgctcaa tttttcccac tcggtgggac tattaaacca cgaaggcgcg 112680

acctcaccgc gaccgctgct gttcgacgtc tgaggttgtt gtgctatacc gcccccctcc 112740

ctcatacagg caacgaaacc cgctggcgca ccacaaggcc tgcgtcgttt cggcgcctgg 112800

gacggagacg ggagcctatc gtcggccatg gtgtccgggt tcacttttgt catgtcttcg 112860

gccatatctc gagtctggat cgaatgcata acggatgctg gcctgagggt ctttgaagcg 112920

atgcatattc gcgtttccgt ggcaggttct gtcatttggg tctccctcgt tgacacagcc 112980

agtacctaca ggcgcagtac taaagctcgg cttcccgccc cacgctgaat ttaaggcttt 113040

ctgaaaagcc ttgaccccca cgtcatcacc tattgcaact tcagagtcca gcaaaaaccc 113100

agaaatcgca aggttagggt tgacccataa tgcccgctgt ggacgctcat accacaaaac 113160

tacctcaatt tggggacagt gactgcggat aaatactcca gacaacaact ccgtgctctt 113220

tggtggtaca gctgcatagg ttgaagtctc cgaggtccct gggcggaggc agtccctaga 113280

tagtatagac aggacatctg gagcatacat aactccttcg ataaccgggt aactacagga 113340

agtttcttca aaaaaaccca ttacgactag gatcacgagc gccaatctat gtaaagtcca 113400

acccattgtc ccgcaccaca agttcggaac gtttcgttct tcggccaccg gacgcactac 113460

gtgtataaca cgatgccgta ttatacctac aagtgttttc aagacggcct tccatctgga 113520

acccattatc gcagttgtag actagcccgt gcctatgtag ggtcgctggg agtagtggcc 113580

tcttgcgcga acaggcccca atacttcccc gtttggagaa tggcgacctt taaagggagc 113640

aaagtcttgc gacgtacgcg cgccacgaca tacgtatgtg caatataaat aacaaagacc 113700

agttaatgtg tgttaagaac ttttattaac caattggcaa tcgaaacata ggatttgtaa 113760

tatgaacaat gtcaggttta cagatcacgg gccaattttt actagaagcg catgtgcgat 113820

gttgtaaaga gtttacaaat tggaccaaat taacggttag gcatgtcgtt acaaaattat 113880

ttctgataca ttgttagctc gttaaagcgg tctggtggtt gtttagtcta atgtaactct 113940

cctacaaatt acccgacacg cacctacaat ttacacctcg gcgacaacat caacattact 114000

aaccccctct tttatgtcct aggtcgcctg ttacccatat tattgtcgcc taatgcggtt 114060

cggtatctga accccagatg gtctatttgt gttatgacga ggggtctggt ggcctcgaag 114120

gcgagcggtc ggaacgacct gatcgtcaac cgttcgcctt cgttgcaccg gtacctgctc 114180

gtggttaact ggtgagcgtc ttctccgcga accaagagaa gttgtagtac tacgatctac 114240

acgacgccta tatgccggtt cgtcatcgcg atcagaaaat tcatcatcag attcattact 114300

atacaacacg gcacgcgcgg ccgcgagagc agcttgtgta cgtgttatga gctctgcccg 114360

tctggctgac gcgcgtgtat acaggacttc atcatttgca ccatcgtgtt catgttcttg 114420

agctacatta cttccagatg gttgttcatc aaaactatcc gagtctaaca gatacacgtt 114480

ccccgtaacg ccctgttcgc catcatccat tctatcttcc tccccaacat ccgcatctat 114540

attgttatct tcctccccaa catccgcatc tatattgtta tcttcttccc catgcccccc 114600

ctggtcttca gctatataag cattatcact gttttcctcc ccaaccgatt ccctgtttct 114660

gttgcccgtt tccctattct catcccgtcc gattcctaaa aggacatcaa ggcttacaaa 114720

ctcttcatca tcgtcatcaa catcttcttc tgatgacgat gatggaacta aaatatcatc 114780

caaatcaagg ctctcttgtt cctcgaatgg cggtagatcg agaatgaatg gtggtatatc 114840

gcccttaatg tattctgtgt acttctgcaa aggcgccacc gttgctgtta cttttccgtt 114900

acagattttg ggtgaagaac ctggggttag tccattaatt acccgctcgt ttgtcaaaat 114960

acatggagcc tctgtatatg ccatcagggg tacacacata cgacgcgcta aagtgtctcc 115020

tgcaacggta aaataaagtt caccaacgcg cggcgcccca taagcacaat gcgtatcatg 115080

tgggaggtga aacatacaac accaatctaa acacctgcgc attactttcc aaaaagccca 115140

agcttccgaa cccgccatta ctgattcaat aacatttcga agccaagttc tattttctct 115200

ataaagttgt ttcagttgtt catgttcgct aaattgcggg tatgccataa taaaccgcat 115260

agctgtggcc catgttgtag caagcctctc tatcataatc ttatcaacat taccatgaaa 115320

cacggcttcc atagccaacc ccctacaggc catatatgcc ccgtctatag cagcaataga 115380

aatcgcatct gcatcatgct ctgatccccc tatatgcgca cgttcgtgca tcatcactac 115440

acgcatagct gtgcgtagtt cgtctggctt cttggttagg ccgtcctctg tccgaaggcc 115500

cttgatgata tccagcgtag ttggaaaaag acctgtttgt caagagaacc acataagcct 115560

cgagtaaaac ttaatggccg atttctatgt tattgtcaga cagagggcta gccaggccca 115620

tgattaggaa aggccataga tagagaagtt agtaaagcca gaacgagatt tgtgatcgta 115680

gtaaagttca tgcaaagtag gtggataggc aaaagctatt atacgataat tagcccccat 115740

cttatttact cacgggatac gatttgctgc ataaatctta gcctatagtc tgggacactt 115800

tcaggcaatt tcagcccatc tgggtctctt gcatgaagtt ttggagcctc ccgcaacgcc 115860

attcgtaaca acgatcgacg agtgtcagaa cctggccaac tagtcttaag tttactttag 115920

aagaaccttc tgtaacttac acacggaccc gcctcgagca ttatggcaga ctaaacaagt 115980

tacccaatat tttactaaac aaaaatggga ggtaacagga agagtagcct catttgaata 116040

acaaccaatg ttagaatttc ataaaaatga aagcaaaata cgccgaaacg gcgccattgg 116100

ggccagcgga ggggatggtg agtttgaata aaaccacaat cccccctcac cgatcccaaa 116160

ggcaccgctc tttcatcgtt cgaaagtcat taacacaaac tagtaagaat atatagtaga 116220

tataccaata ccgcggccta tttagcaatg ctataaatta ttattatact tattggttct 116280

tttcataaaa ttgttattca taccatattg gctaatacgt acttaaacgt tttgttaccg 116340

agcgcccata aaaaagaaaa tgctcattat ttacgtgtct ccgccgacac cgggggcaca 116400

tcatcttggt ctctttcaca cctaatttaa ttagctggac tagctccgcc gctactgttg 116460

gttgtcattt cgtcggccct gttgatcaat aacaattaga tcccgctaat tgaacattag 116520

acccacgaac cgaaaaaagc ggcgggggtc aaatcttctc atattttgcc caattaatta 116580

attatcacca atataaagct attatatatt acagttaaca ctacacaata tatcgctata 116640

gtctatgtca ctattaaccc tccaatcatt aatatatttg cgacttactt tatcgcccgt 116700

caaccggtcc ccggacaccc gcgccttaaa caactagaat acaatttgtt actcaccagt 116760

gcatgcacat gcaccccccc ctcacaaata aaacgaacag actttgaacg actcgttccg 116820

cgcaaattta acccagaata cgatcttact ttcactttag aaaacggtcg gttcctatgt 116880

aacagattgt cgctggtgtc catacataca ttttttattc tcccgcaggc ccctaaacaa 116940

tccccaatat accggagatc tatttagccg gttcatttcg aaccgcaata tcgccggccc 117000

gcaatactgc cctttgcagc tatcccctaa ggtaagcatc cccacccaat aaaattagtc 117060

ccctttaaat actatgtcgg caagcctcct taacgccgcg gcggtaagaa tgacaactga 117120

gcggcgcggc gtcgctcccc ccttttatag tctgaggttg cgcaagctca gtgttaaaca 117180

gtggcgcccg acattttgtc atgagcatgc gcagaattac atagtgaggg aaacaagtga 117240

cgtatagaac gaccgccgcc catagttatg actttgcatt ttgaatctca gcgcagcggc 117300

actgtaaaac cgtttttaaa cggcatgggg ccatatagct ccattttata ttcaacacta 117360

gcctccggaa tgaatactga tgcagtacgg tcatgcgctg cggtgcggta cgtttgttta 117420

gtaatgtata tatttgtgta tagacgctaa caatggccgc ggctgtttgg acagtatatc 117480

ccatattgcc atgcgtttgc cgcaacttct acgccagcgt atgtttaaat ggcgcgatcg 117540

gagggaaggg gggggttaat ccaagcggag tgagataaaa aaggacgcta tgtttggaaa 117600

ttaactcttt gtttattgct ttatatgaac aattagtgtt ctgttactac cgtataggct 117660

tgtccagcac tgttacattt gcctcggttg ttccaaagtc tgcttttaga tatatagtgt 117720

aatttccact atttcggaat aataagcgaa gcatcggtgt tgttctgtca aaagccataa 117780

ctatcccgcg atctctggaa atgttgcaaa tatcccaaaa cgttaaaata gtatctgtct 117840

gtcctgtaac cgtgttgacc atctctattt gcataacctg tccgtgatgc ccaggataaa 117900

tagtaactga gctatttact tgtgttgata ctggaggtct gactgtcgtt aatgtaatgt 117960

tagataaatc gtcatattga aggcaacctt gtagcggcgg tacaggtata gtttctgtta 118020

cagtgagttg agatctgtat ataggtatat agtgttccgt ccccgcagat tcacatttaa 118080

aaatggatcc ggcatgtttg ccaaattcat gacaattata cagtgtcagc tttctagttg 118140

tcattggtag cttgtgtata aatgtaccat tttggtattc ccgcaggtat ccgcgaccgc 118200

cggaaagatg aacggataaa atcctgccgc cggaacaaga aatgaataga ggttttttgg 118260

tatcgtatga accatgcatt aaagcagccg cgacagaaaa agagcctaat gtatatgagt 118320

atacacccgt agagtacata acattaatta tcgaaccact tgtagccatt actactacaa 118380

gattggatgt agtaatatct gtactatgca tttcatatat ataccattct ggtctgtatc 118440

cataatatga aatgtaccta taatctaaac cagacacata agcattccat acagagtata 118500

caagaccgta ttgtccctcc ggagacctgt cgtccggctg caaggtcacc agactttgtt 118560

caacatccaa taacttcata tattgagccg ggccgacaaa ttgcgaacag agcagagatt 118620

ctgttggcgg gcccagtcgc ctaagtattt cttcatgtgt taatgtttca caaaagcgct 118680

cattaaaaaa attagtacca catattctcc gattccattt acgtgtagca gcatcacatt 118740

tacgccctcg tggtccacca agctttgcta ctatatattc atggccagtc agtagtttac 118800

ctggcctaaa aaagtcttgg ttagacgcga ataccactac atattctgca tcctgtttag 118860

aaattgtatt aattttagat gatgtaactc gcgattgtat ctgttgtata gtcagttcag 118920

tggatggatc aatcgcaact aaccgtttac attttgcccc agttgttgag ctaaagtcgc 118980

gacatatcgc cgaacataaa taaccaccaa tactatggcc tatacaatga agctttgtct 119040

gttctacatc tagatttaga aagagaaaac tcaactcgtc tatcattgca tccgcaatac 119100

gatcatcaaa gctgcttctg tttctactct cggttgtcca attaatgaac aagaccgtaa 119160

gtgttggtgt catacgttga tggaagcgca agaatgacat gaaaacattg tctgcgaatt 119220

taactccagt ccatccatgt gtcaaaagta ttatatcccg cccaggtcct agaacaccgt 119280

ctgttataag tgtgttatat atcgggtcca atagtgtaat ttatatatgt ttctgtagtg 119340

atattccagt aatgtagtgt agcggaacct gccgcctccg gcatgtattg gttctgtagt 119400

gttgaaacct tgtgttgagt agccttgagc gattccccat cccctaccac acgtggtcgt 119460

tgtttagctg taacggtcct ggtctgtttc cttttacttt ctttcacttt gggatcttct 119520

gttttcatat tagccatgga tgtgatagtt gtaattggtt gttgctcact cgaggcgtat 119580

tgtctaatgg gggcgacggt tgtcggttct tttccctgaa gttggctttg tctttcgtcg 119640

gtagactctg gtccatgtcc tattatgcga tctaaaacct aagtaaatag aatgggggtt 119700

gtagtaaatc tctcgcgact ctaaataatg gcaattgttt tctgttctta aagcagatta 119760

cttaccaaga agaatccaaa tattatggcg atgataatca gcactacaat gccgaagaat 119820

atcttacacc ccagtgaaac ctcttggcgt gttgctcgcc tcttttggcg gacgaagaca 119880

acatcgaaac gtgttccggc cgcaccattt ctttcacaac actattcctt agcgttctac 119940

actttctgag gtaacatcaa ccatatcaac accgcgtctt tataaatgtt tccctacaaa 120000

ctcctcccat gtgatgttaa gtcaacaaag gtttaacatg ctcattggca gtatttatgt 120060

gtgtatacat tgacacagat gcttatgtat ttatcagacc atgggaacat ttaacacccc 120120

ctgtatgata caaactccat cacggggtgt tagatgaacc caatggtatg atgtttgcaa 120180

ctagcgcgcc atatttatag caccaatatg caactagtgc accatattta tagcaccaat 120240

attagtatag actatatctt tataatatgt catgtagatg gtttagtacg tgcgcggccg 120300

cggccgggag aggcgttgga ccccctttgc catttcccgg gccatcgaaa aaagggggcg 120360

gggctttttt ggcggggggc caaaaggggc gtggcttggc aacatgccaa ctccacccac 120420

tgggcggggc ttgggctgat gccaaccccg cccacaataa gcccctccct tgggaggagc 120480

ttggatcatt gccaaccccg cccatgggag gagttagggt tggcagggtt ccaaaggttt 120540

tacggtatta gggttagggt tggccgggct ccaaaggcag aactgtctta gggttagggt 120600

tggcagggtt ccaaaggttt tacggtatta gggttagggt tggcagggtt ccaaaggttt 120660

tacggtatta gggttagggt tggcagggtt ccaaaggttt tacggtatta gggttagggt 120720

tggcagggtt ccaaaggttt tacggtatta gggttagggt tggcagggtt ccaaaggcag 120780

aactgtctta gggttagggc tggccttaag ccaagtaatg ttattggata gtgttggccc 120840

taagccaagt agggttggca aggttccaaa ggcagaactg tcttagggtt agggctggcc 120900

ttaagccaag taatgttatt ggatagggtt ggggagacgg gactccgaag gtagttcgct 120960

cttaggattg gccccatggc aagcggggcg ggtcttcatc cgagaactcg gcaaaggatt 121020

ggcaatacac caaataggcg gcgactgcat ctgatttagg gttggcattc gcccaagtag 121080

gaaatattga gccgaggcgc taatagggct agggttggcc atacgccgag tagatggtga 121140

ctgtagccag attctacctg gcctttatcc aagtaggtac tactgagccg ggtagcatag 121200

ttattaggac tgggggcgtt gggaggagtt ggagctaacc ctaatccaat tagatgcctg 121260

taatggcgtt atctttatca gagctctgtt atcactttat tttacctccc tctttttgcg 121320

tcaattgcag cctatgtgtg ttaaggaatt aggacctgtg gattactatc atgaatgtcg 121380

gccagcctct ccgcttggta caaacgaaat ataaagcggt agttgcccga cagccatgat 121440

agtctcccat gaacttcggc ggtttagtga tctttgtaac taatattcct ttacggactt 121500

acatagtatt tagacttttg acacttattg ggatggggtg gatggtttgg agacagctgc 121560

ggtggtctat acatctaata accatccatg caatctccaa acaacctacc aattccacca 121620

ataacttttt ggcccggcca tattcccgcc cgtcactttt aaataagaac tacaagacag 121680

attgcattta ttaaaaagac tttattctta actatcgagc tgaatatgtc gaagacctct 121740

atttctttaa aaactggata catagcgaag ctattttctg tgtataaaag gctgtctaaa 121800

aaaatggaga atatataata cggtgcaggt aaaaaaaatc tcttaacaaa aaatcagaaa 121860

aagtcacgac aagctcccgc gtacggattg ccctcatccc ccgaatcatc cccatcggac 121920

aagatctcaa tttttatctt atgtggtgct ttgggcattg gagttataga tggctgtacg 121980

gcagtaattg taacatcatc ctcattagat tgttcattgg gcgggataat agcctgcttc 122040

atatctccgg cctcgatgct agtagcccta gaccccccgt gacatagttc ccaggttatt 122100

gtaggtggac gttggccgcg ctggatagta tttggattga ccacacatac attaccctcc 122160

atttgcggtt cgagtaaagc agctgcacag tatgagcgga ctacttctgg aatgtcatct 122220

ggggtctcaa aaggatgctt ccggctctct gtcaggtaca aaggcctcag aggtgctcct 122280

agaccccatc tatttgctgc cctatgatta taagcagccc cttctagaaa atctggtccg 122340

cttatactga gtccatctgc ctggaaggcc atatccttgc ggtcgtattg cggaagaaca 122400

aggccgcgat attcacgggg ggtcattggc acgcgagtag tgcgaccggc ctttgtctcg 122460

acaacatatt taacattatc acctctcatc agctgtaatg ggggttcatt agggtatagc 122520

tgagcgaacg cggcttcgac ccgcgcaaag actccaggcc ccactatccc acgtgatgtc 122580

atcactaccc ttgacatggc ttcacaatct ggccatctgt gggccccatg ggcctgggga 122640

gttatagtcg cctttatata tgaatgatag ctactaattg ctggaccgtc ggcgggccaa 122700

tccactgtat caattgtatc caggaaaatg agccgcttgt gggccgagcc gagcctgagg 122760

catagatatt caaccgcccc cgcaaatcct aaatctctaa cggatagcag cagcacgccc 122820

ctggaattga gtgcagaaat atctgggacc ccagtccagt tacccgccca tgcgtgactg 122880

ccgtatgaac aaaggcggtc acacaaggcg gctagcagga aggagaggcc acctttttca 122940

tcagaccaca taggcatgca agaggccact ggggcggcgc ttaattcctc ccctacgagc 123000

gggcagtaca tgatgaccac ctttacatcc tcagggtctg gaatctggtt catccaagca 123060

cattgtttgc ggagcggatc agtgactcgc aggggcccgt atagtcttcc ttcgacatgg 123120

cttccgccac gtcgtgccgc cacgcgggcc agtgcctccg gatcaaacat taaagcgggt 123180

ttccagttag gaggaaacat agggtcatct aataattctg cgactatgtc gggctggcaa 123240

taggtagacc gtgccgactc ggttggcagc ggagtatgtg ttattccggt ggggacgcgc 123300

ctatatcccc cgcggggatg gggcccgcct tgcggcattg gaccgagagc cttgaccgac 123360

ggcagtgtcc gcggtaaccc ggtttggtta tcctttttag aattttgtat attctgatgt 123420

tttggcaagc catctggtct atctcggtcc tttggggcag tatcatcact taacacccag 123480

gatagtttgg cgccattgat cttggctgac ccatttccgc tcggggcaga aaagagcgcc 123540

tctgttaggt tccatgcaga atatctacgc ctagccctgt ccacggaaaa gtttacaaag 123600

ggtctcacag tgctaatcac cgtaccaaca gcacgaagcg ccataacaca tgattcaatg 123660

ccctgcgacg atgaaagctc gccccgtagt ctcatagcat ttatacatat aggcaacagt 123720

ttaaggtcct ttatccatga tagaagctgg acgtgatgcc ggtggagctg ggcagacgga 123780

caggcagcgt ccagctccag tgaggaaata ccaggaatgt tgccggcacc accaaagcac 123840

tcgagcaata cctctattac tgcattacaa gctaaaacgc acgcatctgc cagttcctcg 123900

gttgagtaat cagatgcatg ttcgggatca caaaagtcgg ggtccgccgc gctctgctct 123960

gtcgccagat ggtccgtgga ctgcggtgtg ttctgcggct ctatctgcga gtcgccgcga 124020

accgtcacag tctgcagacc ctcggtagct tcgcgcatgc tgcggcggac agcattcatt 124080

cggcgataaa tggaagaaat gtcatccgtt agagatgcgg aggcatcaac ggcctttgcg 124140

gggaaaagca tgtaagcata cgccctccgc agagacattt gcagaaattg tttttgcact 124200

ttatcatagc ggcggctcat cgctacggcc gcggcaacat gcggtagacc ccatagagca 124260

tttccagccg ccatcgcatc tccaatatgt ggtagaggtc gcgcaacact tccagtaata 124320

aatgacccgg aaccagtacc acccctcaga ttgcatcgtt tttgacaaac ttgctccaat 124380

aacagatcct caccagttat tttgttacat tgtatccagc ccatagcatc gaaactattg 124440

ctatatagtg cttttacaag agcatcatac tgctttgatg aatcccccat ctctcccaca 124500

aaaacagcca ccggaccgcg gctctcggca taccgctctc tggcataccg tatctctggt 124560

tcatcccaca gcccctggcg atagtcccca gtaccgccat accgcacctt gccaggtggc 124620

ggtggctcag acccgggcca tggatcccca tttgtcatta gcaaaggtcc gttcatagta 124680

ggcatttctg ggacgcctat tgacatacaa gactcagctc cggcgctaga tgatgacact 124740

cttttcatcg ccgatggccg tctcccccta cccctacggg ggcgctggta aaatgcgtta 124800

tttgacgctg gaggaggttc gcaggagact aaatctatgg tgtcatctga tgatgggtgt 124860

tgatattgag tggactcgcg agggctattc gcgagcatag ttggttgttc catagtctgg 124920

ggctgctgtt cggtttcttc ttgctgaact gcgcggcgta cgggttgctt gcccttgcgc 124980

tcggcggtgc ccgtggcctt gcttgcagcg gtgttactgg tcggtgggtg tgttaattgc 125040

tgcgaaaaca gttttgataa atcagtcttt ggggaccgct gctccgcatt atccttttta 125100

cgctgcatgg cgggcctacg cttctttggt gcagatggaa taaatgatgg aggggggcgc 125160

tttggtgttg gagggcggtc tggggatggt cccctagaaa cagagctccc gtccgaggag 125220

gaggaggacg acgatgtgga tgagctggaa ctggacctgg accgcccccg atcgacagat 125280

ctgcgcggcg acgcggctgg cgagcaacat gagactatag gtagcgcggg cggcgagcgg 125340

cccctggggg cagaccttgg cgatgtagac ggggggccgg aacgttccac gacaccagtt 125400

tgaggcgatc gcgccggcga cctataccaa ccggccgagg taacagaggt cggtgatgac 125460

ttgggtgtca tggtgcgcca actgtcggca catctagcag ggggtggcga tgccgatgaa 125520

gatggtgagc gatggcgcct ccgagcggta ttcttgggcg gtgctgctgt cggtgaccgg 125580

gcaggtgaag cgatcggttt atcaggagcc gatggtcgcc ccgcggtggc agatcttggt 125640

tttttaacac ccactgctgc ccccccgtta tcgctacatt cttcttcaga ttcaccaata 125700

tcaataacat cagcaatgcc cgcataattc ttaccaccgg ccgccacctt cgttttattc 125760

acaccgggtt ttttagccat tgttttcccc ccgcgaccca atttgtactc actctttctc 125820

ttcttttgca gatcggaccc ggtttttgga ccgccttcgg cgcgcacaac acctttgggt 125880

gttgtgtccc ctttctgccg catcccctgt gatggagatt gcattttagg tggtgtggcc 125940

tcttttatgg ggtttaaatt gccacaggtg gttggttctt catcactcga atcaatttct 126000

gtagggccgg gattaggttg caggggttta gtaaatgcag cgtcggaaag cggcggtccc 126060

atataatcca aataaagtcg cccatctcct ctattaaccg tttccatggg tttggtagtt 126120

gctgccattg ccgcagcatt cattacatta tctataagac acctcgagtc gtccagtaag 126180

tactgcgact cgtcaatcaa cggtgtattg ggcccctgga tgccgtgctg aatatcaatc 126240

gaagaaatgt tgctcataaa actacaatcc gcactcgcgg cggcctcaat caggtcagta 126300

aaatctgggt actgcgtctt ggccatgagt agagctgggc gtctattaaa aaagcataaa 126360

gtgacaccag ataagaactg tgcctatttt acaaaataat aaatctggaa gcggataata 126420

tctatacatt agtttaatta atatgttaac cgctattaca gttaattaat aggactataa 126480

gtgatcaatt acttgtagta attgagatta agatattaag attctaaata cctcaaaggc 126540

caaatcatgg atcaaattgt agtatcactt atcaaataaa aaatagttgt aatatttgca 126600

aagaaactta cttttagtct ttagcaatat gtcccagatg ctactccgag ccgtagaact 126660

gcagagagaa ggctgaagag cgctggttag accggagttg aagataggtt gatcgtagta 126720

gaactatgca acgatcgaaa aggcttctac ttaaatgcta ctatagaaag ggcgtagtct 126780

ttagccgcgc ccatacagga aaattaacga tgccttctta gttcgaggga aaaatgaata 126840

catttgccca actacaaatc gcggcccaca tagttgcatt ctattaactg tccgccaacc 126900

aagaagttgg agaagttccg caaagactca atttgcatgt tcagtagcat acgattaagt 126960

tttttccaat gataatgttt cacacagttc ctgttgtgca tattattatt gtgtattatg 127020

ggtaagttta cctaaggtgg ggtaaatgtt agcatatagt taaacaatca ccgataacat 127080

tattactatt cgtttcctga tacgaatata attaaagccc gctccaataa tgttattctt 127140

actatttcct gaatgaatac tattatcggt ggttatgggt aagttttctt agggcgggta 127200

aatgttagca tataattaaa ccataatcca ataatgttat tcttactatt tcctgaatga 127260

atactattat cggtggttat gggtaagttt tcttagggcg ggtaaatgtt agcatataat 127320

taaaccataa tccaataatg ttattcttac tatttcctga atgaatacta ttatcggtgg 127380

ttatgggtaa gttttcttag ggcgggtaaa tgttagcata taattaaacc ataatccaat 127440

aatgttattc ttactatttc ctgaatgaat actattatcg gtggttatgg gtagatgttt 127500

gcgtataatt aagcaacaac caataataat atttctaaaa catcctgcag tgcatatcat 127560

tatttctaag cctatgatca ggcagcatta ttcccgacac ttatatacct agccatgtgg 127620

tggcgctctc acgagaagtt atgctagctg gcggtcatgc gaagatggag ctgcgccacc 127680

tacatgacca tcgtattaac acctattgga gcggcatagt tgaaccgtcg gctacgaacg 127740

gtaggtttca atatagagaa ctctctactg ccatctagtg cgcttataat tatatacagt 127800

gacactgttg cttgattaac cccttcgcga cagtgtgcaa atagagaggc atcactatta 127860

atactatcac agatcctcta gcgagacata gtaataataa cggtgtgatt agttagcgtc 127920

attgctgcta ttctggccgt aatgcggtga cgcatcgcct atattgctaa ctaggggtta 127980

gttatcaaat cctgactcgt atttcctata ccactagact cgttacgatc ggaccgcagt 128040

ctatacgatc tatcaccgtc tagcgaaaca aatgagtact atacctcacc ttagctgttt 128100

tctcctgagc tacttttcta acaccatcta gcgatgcaac tgagtactac atatcacctt 128160

agctacttta ctatttcttc ccctgccccc atctaacgat acacatcacc ttagttactt 128220

tacaacacca tctagtgata caaacgatca ttgcaggtga gcatttccgc acggccacgg 128280

ccgtgcagta ggaaggcggg tctatgggca tagaatagac ctatataggt cgtcgtccgc 128340

tacgatgcgt acctagtata cgagacggtt aatttgcggt tacgtggttg gccgacacct 128400

ctcatcagat ctaaaaaatc agagtaaatc ttgccgatat cattggttca tttaaccaac 128460

atcatatagt cgcacatgcg atcgtgtgta tcgattaata aaaataaaaa aaaaagaaag 128520

caaagttgta tgtctctgag tacttactgc gcggcgcctg cgatgttatt cggcaagcca 128580

gatcggtcga tttttcatgg ccgatagtaa aaatatttgc ccagtttagg gggcgggggt 128640

atcgaccccc ccgggcgtag aaacgggcaa aaaaaaaaaa ccaattttgc gtagaggttt 128700

gtaccgtcgt taagacagtt cagccgttcg tgcattacat ttatataagc ctaggattta 128760

ttttgtactc gctttaaaca tactgtgtca tgtttcaaac tttccgcctt tgagcggata 128820

ggtcgcctta gatagagttc aatttgattg ggtgagacat ataggcgcta ttatgttttg 128880

cccgggtaaa tgtttgtcat caaggcggca gtattgacaa agaatcgggg gtgtccggcg 128940

ctgctaatag atcccgtaaa attcaatgta caggcaataa aacacaatga acggaaaccc 129000

tcattacctt ctgtgccccc ggaataggtt atttaaatat aattagcgct gtatgttgcc 129060

attcttatta aaaggctgag tcactatgtt ctgaggaaaa tatatccgtg tacttaaata 129120

cactatgtac acttaagaca aactgagcgg tttatgaaaa accattaacg acactatccc 129180

tgtgaattga aagatatgat ctaggtccac cattcccctg attatatgaa tcggtcctat 129240

agttggtttt gcgagacagc tttaaattta aatttaaagc ccccaaaagc catcaactgc 129300

tttattcagt gctggtgtgc cgccagccct caattcccat cccgctgctg ttcggcctta 129360

ctttccagga acgtggcaat aaattaaagc ctattagttt aaagcagctc tgctgtgatg 129420

tagcagtttg ttcaaactgc agcagaaacc acagaaagaa aaaaaaacaa ccagacacaa 129480

aacgttaaga gaatatggat tttattatac tcgcttataa gcgagttaaa aactataact 129540

atgttaacaa gttcaatcca aaatgccaga cacaaagtac tggctaaaca gtttaaaaca 129600

cgatcatcgt taacgccaac acggtacagt tgaagctcat acattaagaa acgccagatg 129660

gcaaaataca agatgagtca acaccgaagg acaagatcgc tatcggcaaa ctttcattca 129720

taattacaaa ctgtactcct cgaccctgtg ataaaaacgc cattattcgt acaatatgaa 129780

tgacaaaagt cataaaaaaa atcattcctt gcagtcacca tcgtaatgtc ggatgtggat 129840

cccaatctct cttcccccat cgcgatagtt atagtcggaa agaaacggaa gaatttttgg 129900

aggcttaata cgcataacta aatgcagtga tagtcactga cagcggtgca gccgtatcgt 129960

aaaagtggcg gcgggagcgc gacgacgctc gctaagcgac ccctcctcgc gccctcgtcg 130020

ccggaaacgg ttcgtaggaa tggggaaagc atagtagtct gctgccagcg attaggttgt 130080

atcccatttt agtccacaac acgaccccgt tcgaccgttt ttcctctcct cgcgatctaa 130140

aagagtggaa aaaacaatca ttaaaaattt ggcagtgata tgcgaataaa aaccgagtct 130200

cagttcgaaa caatcgctta ccagaaagcc gccagtcatt ctcgcgctat cgtttatgag 130260

aaagggggcc gtgtagtcgg tggtagccga accccgacta cacggccccc gtatcgttac 130320

gtcttgaatg gtcgctccgt ctgtaccgcc ttgacggtat ggactaaatg ggctcgggtg 130380

ccgcttttta tagtctcaaa gcgccttcaa accacgccca cttaccgaac ccggaccgtt 130440

cgcactctgg tttccaataa tatattataa tatattattg gcttcagagt gcgaacgcca 130500

gatttgacca atcgcattag cccattctta ccacatgatc taattaagca catattcgca 130560

ctccgaaata ctgtgttgga aaacggccaa ctttctattt cacagtgcga actctaatgc 130620

atattcataa ccccacgacc acgcctttaa agttgaatat tattaaggcg tggttattcc 130680

cagagctgcc ttcaaactgc cataaccagt tgacgcaaca taaggtaggc gatcgcgtgt 130740

atataattat atcattgctc aatacgggaa gtattgctta atatggttca atatgaccgt 130800

aacttgacct ttcctcctct caaccaacca ttttacaggc ctaaaaagca ccgcacgcta 130860

gtgtctatac tgttcgacga tacttcggac gaccgtaccg cggacagaat tgatatacgg 130920

ttcgcattcc tgaataaacc attgaaacct cgaccattat acgatcgcgg aatcgcgttt 130980

aggatggcac caaactaaat aaatgattca tggtcaatat ataaaaggct ctcgtttaca 131040

gaactgcgat agacatggcg acggcatcgc gacggccaag cggtcaagcg tgcgaacaaa 131100

ctgtaacggt atcgtatgtt caaggattga gtaattcacc gtctacatcc accgctttgt 131160

ggtcatcacc accggcaact ttgaccaaac cgtctataga atcgcctaga agacaattgg 131220

atccgcgcac ggtaaagccg aggcaacaaa tgtcgccggc tggaggagat ggcatgtggg 131280

atctgcataa gtccatcact gtagatcaca aggaatacgg acctcctgta ccgccggacg 131340

aagcccgcag tggtatggat cccaaaatgg gacccggcgc gttttgtgct tcgccgtggt 131400

tacccgacgc aactcgacta ggtgacgatg ttaacctcgt gtttaaagct ataatacgag 131460

catccggatc aactgggcgt tattgccggg cctttcggag aagtttattg gaattttacc 131520

tgatcgggcg ctatggacca cgactgccgc gcgagggctg ggaagctact ttacaactat 131580

cccccaacca aagtggaccg ctccgtgctg tattgcgcga ggcggacgga cgtatagtga 131640

acaataccgg ctatatggaa gggccgtttg ggcccaccga agcgccgtat ggagcagaat 131700

gtgaggtcga cgggggatgt tattctgacg atgagcgatc ggaatgcgag tcagatctat 131760

cggaagggga catgaacttc gtgttatcgt cagatacgga aagcgagcgt ggctacgatt 131820

ccgacgcgtc ggtacgtagc gtcacatcaa gcagccatag taattgtgac gatgttgact 131880

atgacccgct gggcgacctt gattcagaag aggagatgac ctcgtcggta tctacctccg 131940

actctgaaag cgaagttata gaggttatag ggaccaaacg caaacggccg accacgagga 132000

gtatgagcgc actcgcgggt accacaaaac gccccaagag ttaataaacc tgtcgcgata 132060

tatgctgcaa gcgcgtatta accttttgta gctatgcaac tcgttgggta ccgcaaacgc 132120

ccaagagtta aaaaaaactg tcgcgatata tgctacgggc gcgtattgac cttttgcgat 132180

tgctatgtaa ccagttgctg gctcctgttt gccccgccca ttccatccaa ttgatgtcaa 132240

taaacaccgc ttaagaaacc gacgccggca cttgtttact gaagggaggg acggttgggc 132300

gtgggcgagg gcgtggggga aacggccagc ccgcggcggg tcgggtaaat ataaatgaag 132360

aggcgctgtc tcaatcttat tgtttattag tatactaata acaataacag acaaatgcat 132420

gaaattacag aaaatatgaa aatatcacaa atggccgacc accaagtagg tttcatacac 132480

tctgtgcggt gttcaggtag cactatattc ttcgcgcgat cgactacacg acagttttcg 132540

aatagcccat caacataaca aggatgacaa atgtaacggt cgccgaggca tatgtctggg 132600

cgtaatggaa ccgaaagacc taagttggca ttaacacaaa gcgagtactt ctctgcgtcg 132660

gtcattatat tccatctgcg tgaccggtct gcgcttgata gtggtctagt gaggcactgt 132720

cgccggctag tacatctgcg cattccgaat ggattccaga gcagttgtag gtacaggtat 132780

caaacgttct atcccttgta ggatgcggta taacccctgt ataccaggtg tcggcctaat 132840

atacctgtgc attagtaata caggtaacta tttcactagt gagtcattag ttcaacatcc 132900

atgatcagat gcggaaacta gatttgcttg ccctctgttc atgaggatat ggtggaacgg 132960

agaactcgtt ctcaaaagaa tcgtcggaaa agtctccacc attttcacag tcactatcac 133020

tgtatgactc gacgccgggt aatgaattgt aatacgagcc gtatgcattc ttgatgccat 133080

tgcttttcga acgcgctctt gccctaagga atttagtagt taaaaagatg gatgaaacag 133140

ccaggattat aatagtcacg acccctagta ctccgagacc gactacaaac agtccagatc 133200

tgccgtcatg ggtaccgcgg ccaggtgtag attcggtaga ctttagcatc gtatcttcag 133260

caattgcggg tttatgagtt tcgatggtca cgccgacaaa tagatcaata gttgatagta 133320

acgtatatgc ccacgatgtt attttatcat ctatcgtgat aattacgaca tatagtcctg 133380

agtctgtgtt ctgaggattc gtcagtctta accctatacc cttttgcatt acttgtattg 133440

ccgcattatt atcaccgtct agctgttccg attgacatga tgcccaacca gcacccgtct 133500

cattgcaatt gctgatcttt ctttccccga ctatactggc gttcaacgta gatgcgaatg 133560

cgcaatttgc gttttctggg aaaagacatt cacgttcaga cggatgaaat aaacacggtt 133620

cataaagcga tagtataggg catgtgttgt caactatagg cttcataaac cactggatag 133680

tcatattata aggtccttgt atgtggtaga tggataagtc tgctgtcacg ttcatatctt 133740

cgccggcatg gaataggact gttttataca aagaaatgat agcttatgcg tccatatgtt 133800

taggtgtaat aggagcatat gttggcattg gcatcgcagt cggttttagc gcaccgtctt 133860

taagtgtaag aagaaatact tctgtcctaa aatacgacga atcatttata cgtgcgtgta 133920

gtagatagag tccttcgttt gcttctgttg aattagtgat ctttataccg cgtatgttgg 133980

ctccaagtat tggatagaca ttagaatcgc gggtaaaata agcatgatca agttccgtca 134040

gggctgagtt ttcatgtacg gtgtagtaag cgattcctag cggaacggtc atatgtccat 134100

ggtagtcgca atccttgtcc attaccaaat ccgaaaagca gtagattgtg tcgagacaga 134160

tataggccgg tgctacggct ttgcattccg tagttttggt aaatgaccac gcttctttga 134220

agcgggatcc gacggtcgta ggcaacttat cacgtatttt cggctccagc gttacatcct 134280

gaccggtagt tccaatcact tgatgatagt ctgctataca acagttcaac atacatgtta 134340

ttatgcacac agctgtagat ataaaagtaa ccatcatcgt accaattgtt gaggttccgt 134400

agtctggccg gcagtatgtt ggtgtttagt actccaacct ccgcgcataa tacagttaac 134460

caggctgcac acttaaatta gtacagatct atagctccac ccatagccta ttacctcatg 134520

tacaaatata tttggatgtt aatgaaaggc aaacagtcaa tcataacaaa aacatttact 134580

tttagtcata ctgatgtgaa ttattctgtt ttatgatccc cagagctgtc atacgctgcc 134640

agtttttcct ccagctttct cattagggta ccatctagct ccggatgttt tgattccaga 134700

gcgccttgag aaggaggatt atccagtgcc gattggttga tcacgcttgt ggtagaacct 134760

gtataaacac gtcgtttcgt acaacaacta cataatttgc gtttaagagg agagcacatc 134820

aacacgatga atatggtcaa cggtagtacc acaaacatta cagctgggat aagatagata 134880

tttggaattt tgtcaaagat attaagtcca ctattggtat cattatgcgt aggctgcata 134940

tcatcagata atgttgtgta attgttagtc gtaggaacgg tcacaggctt catggtcgag 135000

aagccttctg tagatacctc ttctttcaaa tgcttgtcat cctccaatgg tccagaaatg 135060

cccatgagat gatagtcgag aggaactcca gaatagaagt catagtgatg gcatatggaa 135120

cctacatccg ctacaactcc gaaatcttcg ccgacgggtg gccgttctgt taccttttca 135180

taagcgttat catccggggg gactactgca ttaattttgt cggtaattat aactgtcgtt 135240

ttaaacacat ccggtactac agcatcgttc acacttactc tgatataata tatccccgaa 135300

tcgtgtatag atggagagtc caatacaaac aacgcagtgc tacttagaac atctttcagg 135360

tgtggtcgcg ccgggtcgaa atagcgtgta gcatgtctgc aggccttgaa ggcagttgct 135420

ccaagacgag ggcaatcatc atatatagct gcatacgcga cagaatagca gtgacgagaa 135480

atattatata ctagtagctc gacagttcca ttgtatactg gcggtagttc ggcctgtgat 135540

ccaagaaaaa gtagttttcc ataaactgcg tgtctgtctt ccacggccgg tattgacgca 135600

gaagcaggtt cctgtagata taagcttacg gacgtcccag catacacgat tgcattgatc 135660

gttgttatac agttaatcgc cgaaacaagt atcagtatat gtcgtgtcgt tcccatttct 135720

atttttgaaa ctcaatatat cgtctatatg gcgcactgga caatactgga agtacgcgct 135780

cccagtaata cactaaaaga actacagacg gccgcaagag acgtgtattt tatgtccaca 135840

taggcgccgc ccagtcaagc ctatcctctt cgtcgaggat tttgtaagcg cttcttactg 135900

gtcttgtatt tttagttttg tggcggcgct tatcatcttt ggttgttaaa catatgtaga 135960

tgacccaccc acaaatacct aatactatac agatagacac tacagcgatg ctaatataaa 136020

ttgctgtttt ggacgtggcg tttggcaaat cctcatcttc gagaccggaa tcatgggccg 136080

gttgcgtagt aattatatgc gataaccagt tgttaggtaa cttaggcctc caatatgatg 136140

gctcatcctg aggactacta gttccaacat agctttcagt gtcgttttcc cggattattg 136200

taatacttcc tccgttaact tcagcattat gtgcatgtcc atggtcatcg ctcgcggctt 136260

cgttatgtgt attactacta gtacgcgtta tgacgttgtc gtctgtatat gttttgggta 136320

caacggcgcc acgctggcgc atccagtact ctactattgc cttgtgcata ggttgttcaa 136380

agtaacttgt gaggcccatc gtcttctttc tatttgcaac atcaatctct ttttgagtaa 136440

aacaacgatt gctggtggtc gggtccggct tcccgaacca acacgatgaa ttaatgttaa 136500

cttcaaaatc agaataaatc ctgtcgccat taacttccaa aacgcgtgtg tattgtccac 136560

tatgccatga cataggtgcg gcaatgacca gtccgagttc gtcatctgtt tggaacatga 136620

aactcgagaa cttaaagtcc caccacatag ctgatctaac tttacagtcc ccaaaaacct 136680

gtcgtaatga acaattccca tattgtatag tcattatcgg gtaggcacaa cccactccga 136740

ctttgaacca agatattgta gcgttatatg tagacatacg gttaagcaat gtccccaaca 136800

ttagtcggtc gagtccttcg cccggtatta gcgctattgt agaacaatca cctgtatatt 136860

caagcgcctt gacatacgtt gcagacccct tcggtctttc aaatggcgat gctatccacg 136920

tgccgggcaa tgtccagtcg gtcttatgag taaaacgcgg gggtggtaca gacggcggtg 136980

gaatatacac cgttgtagtt tttcgttgtg taactgcatg attgccaata gctgcaaggc 137040

tagtatcgct ataataaatt acgatcaaga acaatatatt cgctgagtat ttattcattt 137100

cctactgaga tgttggacca tcaaatcctg ggtcttaccc caattaaaac aacaacagaa 137160

ctgtaatggg tacattaaac atacgcgcat atacatattg ccgtcatacc atacaaaggc 137220

ataggtacag cccacaggtt aaaaacaaag aaactgtagt cattaaagcg gccatggccg 137280

acattgacca tgttgaaaat ccttcataat ttccgcggaa ccactttcta ctaacagatt 137340

cggcataacc gtctaaagtt cttattgaac atatataaaa cacattgtcc cggccttcag 137400

cacgtggcat cgcaattgca ctgtacattc gccagcactt atcccctggt attgttatat 137460

tagtaaccgg tccagattct attttcgttt catgcacgac ccaagagaag attgggactg 137520

tgtcaagatt atcggcctta cacatggcca catgacgcaa ttcgtcaaac tctattgcca 137580

aatgtttacg tgaatagcat gggattgcag catcgaccga tatgcaaact gagcgcccat 137640

tctcttgcag gtgtgaaaat tcggaagcac atatttcgca agttataaca gattttatgt 137700

ccgccacagt ctgattgata tggactatgc caatggtcgt tctgtagtcg ttaacagtcc 137760

aatgttcttt cttagtcgta acatcaatta tagctttatt tacataccag ttaaacgagt 137820

atgtgtagtt atctagcgtt gcgacatgac attctgctct gagagcctta tggggttcat 137880

caggtggaat tatcttcagt agtgtcgagt ctctatcagg aaacggactg gtcgtgttgg 137940

cgaatgctat atggtggtaa tgtccggcga aatgatcttt atcttggtct tctatttcag 138000

cgctttccgg aaagtcttca ccagaattgg gccatctggg tatccatggg tataataacg 138060

aaccattgtt tgtggcttct atatattcat gagagcatgg tgcggcgctt acccatggat 138120

aagcagttaa gctagtgtcg tcgataatgt ctttgggagt agaaaatctg gtatatatgc 138180

cagtatcggc accacttaaa ctatcattta caattagtct aaacagcata atccgccgca 138240

atccgtacga gtatattata gtcttctcat atattttcgc taccaacaaa gaattattta 138300

gtgttttatt catggtaata tttacatcga atcgtctagg ttgatgggat acattgtata 138360

tataatccat gcgccacatt tgggggatcg tggtttatca tggttgaacc attaaaagag 138420

gctatttcat aatagtgcca ggtagagttt tctggcttta aaagtgttat gttaaaatat 138480

tgggtagcgc tacttctatt cctacctcta aacgacaaag gtattgtcac gtttgccgca 138540

ttcggcgcat cgatacgaat acatctcgtc cggttagaga ttattgtgaa gttagcaccg 138600

ttgtaaattc catctggtgg cgcttcagcg gccgaggtac ttgacgtggt ctgcggtgtc 138660

gcggtagtaa ccgaagctgt actcgtccat ggttctgtaa cagtcgatgg gttcgtagca 138720

tctactccca acgacatagt tggctgcggt gaagttggcg agcattgaat gctcatagta 138780

aataaaatca cggctaccca catgaccgta acgtctgtat acatcctgca tgagataagg 138840

actacgacag gaggcctaca tcgcttgcat taaattactc tttcatttta accgtttaag 138900

tcaacattcc gcgtcaatat aaacacaaga cacacacgtc atttaatatc tttatttaaa 138960

aatagctgtt taaacagatg attttaaact aatggaacgc gttggaattt caagtcttgg 139020

cgcccaaaca tcgattgatg tttggtcaat cgcgtaacaa cagatcgaag atatagaata 139080

tacatcccaa gaagaattat tgtcacgata ccaaaacatc cgatagtcac tattgcaatt 139140

aaaccactcg taccgataca gcgccgcgca tcttctacta gagcggcaaa tagtagtggc 139200

tgatctatca tactgtcata ggtatgtggg ggatgagggt gattgtggta gcttccatgc 139260

gaatgtgttg aggtggtatt aatagttgta catgtcgata ggttcgccat tttaagacca 139320

atccgcggca tccttccagt agaatgtacc atgaaactgg cgagtaacaa atacccacca 139380

gtttgatttc tgaaattggc attggtttta ggtactgtcc atatgtccgt ctcatttaca 139440

cattcattat ctatgtcatc atagcattca gatccatgat ctgtagagcc gtagtcttca 139500

taaccacacc gggggaaacc aagcatggcc ggatcgacat tgcgttcatc ccaagactca 139560

ctctcgtccc tgatttcttc gagactcttc accagttgac aaacggtctc tgtgccattt 139620

tggaaactaa atcgtatctt cccgtctttc gtactaatat tatacgtatt gggatggggc 139680

aaaccatctg gagacatcca cgtacctata tcaacaatgt ccaggtcagc gccagacgtt 139740

ctgccaaatt tattaacgca ataaggatcg tctgatgttt caaaagttac atttagtgtt 139800

ccagtcttcg tattattgtc tgttcttaga ataaacttat atagtccact attatacatc 139860

ccactagata taatcaagcc agtaccatat ggtagcgtgt ccatttgtcc atcaatataa 139920

taggaataaa tatgcgaata gccgccacag gtccatacag acggctgatc cccagagcag 139980

ttgtagtatt ccacgtagcc aacaggcatc gtgcaattag cggtgatgtg ataccaagta 140040

acagacatgt catatggcgc accattcccg gcggaaacac tggaaatagg cgcgaggagt 140100

gtaaggtcgc accattgaac tgcaatatcc atcgttccaa tgtcctcata tcgtcctgtg 140160

tgtggtggta tcctcccagg cggtccgatt ggcaacggtc cgtccaaaat agccctggcg 140220

ccagctggta taactacacg tcttgcatgt gtagcgttgt cccatgttcc aagatgaatt 140280

gggtccttgc tgcggctatc tcccattgcg atgcctaaaa cgcatcccaa aaaaactaac 140340

accacagcta tcattgttgc cataccagct acacaagcgc ctctatgatt tcaacactcc 140400

agcggcgcgc ccgacccgga gatcgccgca acgcgcatag ctacttgtct aatttataca 140460

cttttttaca aacttgactc ctcccatata ttaattgtaa tttacccttt gtgggtaata 140520

acggaaagcg aaagcagatc gtctgccgta ggtcgccttt gttgatcaaa tgtcaacatc 140580

ttgcatatca gatattcggc gtccatatgt aatgtatatt tacgtatcaa aggcgggact 140640

gtatatggtt tgcgcttaac ttctgaatac tttccatatt cctttacaat acggcagtta 140700

ccatttactg gaaactcagc cggatgtact tgcatggcct taattattcc acgtaattga 140760

tgactactat acccagaggg gtttttgatc ccaaaaagcg aaacacgttg tactagcatt 140820

tcaaataaca ctattccagc gctccatata tccgtctttg cgcaatatgt atccaaggcc 140880

aacagttcgg gagaattaat ctcaagcgtt ccaacccacc catagtcttg aggtgtatac 140940

ggcgatgccg ttgttttaca cgcagcgccc aaatcgccca acactgcatc attcggttcg 141000

ttgagaaata tattttcaag ttttacgtcg cggtgtatta ttcctcttcc gtgcaggtaa 141060

ctaagtgcct ctaatagtcg tttttggata gtaaatgcgg tttctagact gaggggcgat 141120

gaacgctctt cgacgtatgt atataagtca tgagtataat gtggcaatac cagacatact 141180

aatggaccgt tgcgaaatgc atcaatagca ttaattatcg ctctgtggtt tatattacgc 141240

aaaaaccgcg cttcggtttc cgttccacct ttactgcccg ccttgactac tacgggtgta 141300

cgttgttcat cgccatgttt cacacataca aatacttctc cttctgcgcc aggtgttagc 141360

gttttcaata tttcatattg cgtattcaca accgccgcca cgtcagacga cgaagtatat 141420

tcatgtggag attgacaaac gtcggtctgg tcggccaaag attcctcatc tccatcataa 141480

tcagttgcat gtgtgtttga atcagacgtg aacagttcat cacaatcgtc aaatttgtcc 141540

ccggtgtgat tcttccgatc agacgtgtcg ctcccgcctc gcaaatcgaa ttcggcaaaa 141600

gcgcccaaac atattacctc gtcatcgaac tctaatgtat cggtatgaca cgtttccatt 141660

tcatttgagc tgcgtccgcg gcagttttgt atgtttgttc tcgtttgata tgagcaatca 141720

tctaaatcat catacctcgt tttagacgac aactgttcca ttgccaacga ttgtagacca 141780

caggaagatc ttacgaactc gccctttgac aacaaagtgt ctaaaaggca gagaatgtgg 141840

cttatgaagc agcataagga ggagacgcgc aatgggtgtg gttatattgt caatagccac 141900

cctgattgac gaaaatggac ggttacctgg cagaactaga gacgcctcgg tacatttatg 141960

ggacatttta tttaaacaat gtgccaaaat gatggatgat tcgctcggaa tccctgttgt 142020

tatcagaccc gcaaatctaa ggcgttgtgc cagcaaattt atgagcatgc cacgagccca 142080

cagacctatt ttaagactaa ccaaccctct gggaactggg gcaaacggaa ctggcctcgc 142140

gggaagacgt gatgtaccac cagtacaatt ttatgaagac ggttccgaaa gtacagaatg 142200

gacgagtgtt ttggctggct atgatcatct ttcgtcgggt atacttggga atcatacttt 142260

tgatatgtgg attattggtg ctgccgatat ctgcaagccg gcaatagaac aactgcccaa 142320

ttcaaaacgt ttcattacca tcaaagttcc aggcacatgg tctggtttga cgtgggaaaa 142380

cctgatggtc tatcacccct tactgtaacc gaatgggatc cttgtgacga tgaaaccatg 142440

agcaaagaca ttattgagaa aaagctcgtc ggcataaaat gttgttatga cctcattggc 142500

cagcccgctg ccgcaaaaca taatagcgaa ttggatgaca gcaaagatgt aaaatgctgc 142560

aacggccccg cctgttgtat agcctgttag ctttatatag caagcgcagc tgacaactgc 142620

tctggaatcc attcggaatg cgcagatgta ctagccggcg acagtgcctc actagaccac 142680

tatcaagcgc agaccggtca cgcagatgga atataatgac cgacgcagag aagtactcgc 142740

tttgtgttaa tgccaactta ggtctttcgg ttccattacg cccagacata tgcctcggcg 142800

accgttacat ttgtcatcct tgttatgttg atgggctatt cgaaaactgt cgtgtagtcg 142860

atcgcgcgaa gaatatagtg ctacctgaac accgcacaga gtgtatgaaa cctacttggt 142920

ggtcggccat ttgtgatatt ttcatatttt ctgtaatttc atgcatttgt ctgttattgt 142980

tattagtata ctaataaaca ataagattga gacagcgcct cttcatttat atttacccga 143040

cccgccgcgg gctggccgtt tcccccacgc cctcgcccac gcccaaccgt ccctcccttc 143100

agtaaacaag tgccggcgtc ggtttcttaa gcggtgttta ttgacatcaa ttggatggaa 143160

tgggcggggc aaacaggagc cagcaactgg ttacatagca atcgcaaaag gtcaatacgc 143220

gcccgtagca tatatcgcga cagttttttt taactcttgg gcgtttgcgg tacccaacga 143280

gttgcatagc tacaaaaggt taatacgcgc ttgcagcata tatcgcgaca ggtttattaa 143340

ctcttggggc gttttgtggt acccgcgagt gcgctcatac tcctcgtggt cggccgtttg 143400

cgtttggtcc ctataacctc tataacttcg ctttcagagt cggaggtaga taccgacgag 143460

gtcatctcct cttctgaatc aaggtcgccc agcgggtcat agtcaacatc gtcacaatta 143520

ctatggctgc ttgatgtgac gctacgtacc gacgcgtcgg aatcgtagcc acgctcgctt 143580

tccgtatctg acgataacac gaagttcatg tccccttccg atagatctga ctcgcattcc 143640

gatcgctcat cgtcagaata acatcccccg tcgacctcac attctgctcc atacggcgct 143700

tcggtgggcc caaacggccc ttccatatag ccggtattgt tcactatacg tccgtccgcc 143760

tcgcgcaata cagcacggag cggtccactt tggttggggg atagttgtaa agtagcttcc 143820

cagccctcgc gcggcagtcg tggtccatag cgcccgatca ggtaaaattc caataaactt 143880

ctccgaaagg cccggcaata acgcccagtt gatccggatg ctcgtattat agctttaaac 143940

acgaggttaa catcgtcacc tagtcgagtt gcgtcgggta accacggcga agcacaaaac 144000

gcgccgggtc ccattttggg atccatacca ctgcgggctt cgtccggcgg tacaggaggt 144060

ccgtattcct tgtgatctac agtgatggac ttatgcagat cccacatgcc atctcctcca 144120

gccggcgaca tttgttgcct cggctttacc gtgcgcggat ccaattgtct tctaggcgat 144180

tctatagacg gtttggtcaa agttgccggt ggtgatgacc acaaagcggt ggatgtagac 144240

ggtgaattac tcaatccttg aacatacgat accgttacag tttgttcgca cgcttgaccg 144300

cttggccgtc gcgatgccgt cgccatgtct atcgcagttc tgtaaacgag agccttttat 144360

atattgacca tgaatcattt atttagtttg gtgccatcct aaacgcgatt ccgcgatcgt 144420

ataatggtcg aggtttcaat ggtttattca ggaatgcgaa ccgtatatca attctgtccg 144480

cggtacggtc gtccgaagta tcgtcgaaca gtatagacac tagcgtgcgg tgctttttag 144540

gcctgtaaaa tggttggttg agaggaggaa aggtcaagtt acggtcatat tgaaccatat 144600

taagcaatac ttcccgtatt gagcaatgat ataattatat acacgcgatc gcctacctta 144660

tgttgcgtca actggttatg gcagtttgaa ggcagctctg ggaataacca cgccttaata 144720

atattcaact ttaaaggcgt ggtcgtgggg ttatgaatat gcattagagt tcgcactgtg 144780

aaatagaaag ttggccgttt tccaacacag tatttcggag tgcgaatatg tgcttaatta 144840

gatcatgtgg taagaatggg ctaatgcgat tggtcaaatc tggcgttcgc actctgaagc 144900

caataatata ttataatata ttattggaaa ccagagtgcg aacggtccgg gttcggtaag 144960

tgggcgtggt ttgaaggcgc tttgagacta taaaaagcgg cacccgagcc catttagtcc 145020

ataccgtcaa ggcggtacag acggagcgac cattcaagac gtaacgatac gggggccgtg 145080

tagtcggggt tcggctacca ccgactacac ggcccccttt ctcataaacg atagcgcgag 145140

aatgactggc ggctttctgg taagcgattg tttcgaactg agactcggtt tttattcgca 145200

tatcactgcc aaatttttaa tgattgtttt ttccactctt ttagatcgcg aggagaggaa 145260

aaacggtcga acggggtcgt gttgtggact aaaatgggat acaacctaat cgctggcagc 145320

agactactat gctttcccca ttcctacgaa ccgtttccgg cgacgagggc gcgaggaggg 145380

gtcgcttagc gagcgtcgtc gcgctcccgc cgccactttt acgatacggc tgcaccgctg 145440

tcagtgacta tcactgcatt tagttatgcg tattaagcct ccaaaaattc ttccgtttct 145500

ttccgactat aactatcgcg atgggggaag agagattggg atccacatcc gacattacga 145560

tggtgactgc aaggaatgat tttttttatg acttttgtca ttcatattgt acgaataatg 145620

gcgtttttat cacagggtcg aggagtacag tttgtaatta tgaatgaaag tttgccgata 145680

gcgatcttgt ccttcggtgt tgactcatct tgtattttgc catctggcgt ttcttaatgt 145740

atgagcttca actgtaccgt gttggcgtta acgatgatcg tgttttaaac tgtttagcca 145800

gtactttgtg tctggcattt tggattgaac ttgttaacat agttatagtt tttaactcgc 145860

ttataagcga gtataataaa atccatattc tcttaacgtt ttgtgtctgg ttgttttttt 145920

ttctttctgt ggtttctgct gcagtttgaa caaactgcta catcacagca gagctgcttt 145980

aaactaatag gctttaattt attgccacgt tcctggaaag taaggccgaa cagcagcggg 146040

atgggaattg agggctggcg gcacaccagc actgaataaa gcagttgatg gcttttgggg 146100

gctttaaatt taaatttaaa gctgtctcgc aaaaccaact ataggaccga ttcatataat 146160

caggggaatg gtggacctag atcatatctt tcaattcaca gggatagtgt cgttaatggt 146220

ttttcataaa ccgctcagtt tgtcttaagt gtacatagtg tatttaagta cacggatata 146280

ttttcctcag aacatagtga ctcagccttt taataagaat ggcaacatac agcgctaatt 146340

atatttaaat aacctattcc gggggcacag aaggtaatga gggtttccgt tcattgtgtt 146400

ttattgcctg tacattgaat tttacgggat ctattagcag cgccggacac ccccgattct 146460

ttgtcaatac tgccgccttg atgacaaaca tttacccggg caaaacataa tagcgcctat 146520

atgtctcacc caatcaaatt gaactctatc taaggcgacc tatccgctca aaggcggaaa 146580

gtttgaaaca tgacacagta tgtttaaagc gagtacaaaa taaatcctag gcttatataa 146640

atgtaatgca cgaacggctg aactgtctta acgacggtac aaacctctac gcaaaattgg 146700

tttttttttt ttgcccgttt ctacgcccgg gggggtcgat acccccgccc cctaaactgg 146760

gcaaatattt ttactatcgg ccatgaaaaa tcgaccgatc tggcttgccg aataacatcg 146820

caggcgccgc gcagtaagta ctcagagaca tacaactttg ctttcttttt tttttatttt 146880

tattaatcga tacacacgat cgcatgtgcg actatatgat gttggttaaa tgaaccaatg 146940

atatcggcaa gatttactct gattttttag atctgatgag aggtgtcggc caaccacgta 147000

accgcaaatt aaccgtctcg tatactaggt acgcatcgta gcggacgacg acctatatag 147060

gtctattcta tgcccataga cccgccttcc tactgcacgg ccgtggccgt gcggaaatgc 147120

tcacctgcaa tgatcgtttg tatcactaga tggtgttgta aagtaactaa ggtgatgtgt 147180

atcgttagat gggggcaggg gaagaaatag taaagtagct aaggtgatat gtagtactca 147240

gttgcatcgc tagatggtgt tagaaaagta gctcaggaga aaacagctaa ggtgaggtat 147300

agtactcatt tgtttcgcta gacggtgata gatcgtatag actgcggtcc gatcgtaacg 147360

agtctagtgg tataggaaat acgagtcagg atttgataac taacccctag ttagcaatat 147420

aggcgatgcg tcaccgcatt acggccagaa tagcagcaat gacgctaact aatcacaccg 147480

ttattattac tatgtctcgc tagaggatct gtgatagtat taatagtgat gcctctctat 147540

ttgcacactg tcgcgaaggg gttaatcaag caacagtgtc actgtatata attataagcg 147600

cactagatgg cagtagagag ttctctatat tgaaacctac cgttcgtagc cgacggttca 147660

actatgccgc tccaataggt gttaatacga tggtcatgta ggtggcgcag ctccatcttc 147720

gcatgaccgc cagctagcat aacttctcgt gagagcgcca ccacatggct aggtatataa 147780

gtgtcgggaa taatgctgcc tgatcatagg cttagaaata atgatatgca ctgcaggatg 147840

ttttagaaat attattattg gttgttgctt aattatacgc aaacatctac ccataaccac 147900

cgataatagt attcattcag gaaatagtaa gaataacatt attggattat ggtttaatta 147960

tatgctaaca tttacccgcc ctaagaaaac ttacccataa ccaccgataa tagtattcat 148020

tcaggaaata gtaagaataa cattattgga ttatggttta attatatgct aacatttacc 148080

cgccctaaga aaacttaccc ataaccaccg ataatagtat tcattcagga aatagtaaga 148140

ataacattat tggattatgg tttaattata tgctaacatt tacccgccct aagaaaactt 148200

acccataacc accgataata gtattcattc aggaaatagt aagaataaca ttattggagc 148260

gggctttaat tatattcgta tcaggaaacg aatagtaata atgttatcgg tgattgttta 148320

actatatgct aacatttacc ccaccttagg taaacttacc cataatacac aataataata 148380

tgcacaacag gaactgtgtg aaacattatc attggaaaaa acttaatcgt atgctactga 148440

acatgcaaat tgagtctttg cggaacttct ccaacttctt ggttggcgga cagttaatag 148500

aatgcaacta tgtgggccgc gatttgtagt tgggcaaatg tattcatttt tccctcgaac 148560

taagaaggca tcgttaattt tcctgtatgg gcgcggctaa agactacgcc ctttctatag 148620

tagcatttaa gtagaagcct tttcgatcgt tgcatagttc tactacgatc aacctatctt 148680

caactccggt ctaaccagcg ctcttcagcc ttctctctgc agttctacgg ctcggagtag 148740

catctgggac atattgctaa agactaaaag taagtttctt tgcaaatatt acaactattt 148800

tttatttgat aagtgatact acaatttgat ccatgatttg gcctttgagg tatttagaat 148860

cttaatatct taatctcaat tactacaagt aattgatcac ttatagtcct attaattaac 148920

tgtaatagcg gttaacatat taattaaact aatgtataga tattatccgc ttccagattt 148980

attattttgt aaaataggca cagttcttat ctggtgtcac tttatgcttt tttaatagac 149040

gcccagctct actcatggcc aagacgcagt acccagattt tactgacctg attgaggccg 149100

ccgcgagtgc ggattgtagt tttatgagca acatttcttc gattgatatt cagcacggca 149160

tccaggggcc caatacaccg ttgattgacg agtcgcagta cttactggac gactcgaggt 149220

gtcttataga taatgtaatg aatgctgcgg caatggcagc aactaccaaa cccatggaaa 149280

cggttaatag aggagatggg cgactttatt tggattatat gggaccgccg ctttccgacg 149340

ctgcatttac taaacccctg caacctaatc ccggccctac agaaattgat tcgagtgatg 149400

aagaaccaac cacctgtggc aatttaaacc ccataaaaga ggccacacca cctaaaatgc 149460

aatctccatc acaggggatg cggcagaaag gggacacaac acccaaaggt gttgtgcgcg 149520

ccgaaggcgg tccaaaaacc gggtccgatc tgcaaaagaa gagaaagagt gagtacaaat 149580

tgggtcgcgg ggggaaaaca atggctaaaa aacccggtgt gaataaaacg aaggtggcgg 149640

ccggtggtaa gaattatgcg ggcattgctg atgttattga tattggtgaa tctgaagaag 149700

aatgtagcga taacgggggg gcagcagtgg gtgttaaaaa accaagatct gccaccgcgg 149760

ggcgaccatc ggctcctgat aaaccgatcg cttcacctgc ccggtcaccg acagcagcac 149820

cgcccaagaa taccgctcgg aggcgccatc gctcaccatc ttcatcggca tcgccacccc 149880

ctgctagatg tgccgacagt tggcgcacca tgacacccaa gtcatcaccg acctctgtta 149940

cctcggccgg ttggtatagg tcgccggcgc gatcgcctca aactggtgtc gtggaacgtt 150000

ccggcccccc gtctacatcg ccaaggtctg cccccagggg ccgctcgccg cccgcgctac 150060

ctatagtctc atgttgctcg ccagccgcgt cgccgcgcag atctgtcgat cgggggcggt 150120

ccaggtccag ttccagctca tccacatcgt cgtcctcctc ctcctcggac gggagctctg 150180

tttctagggg accatcccca gaccgccctc caacaccaaa gcgcccccct ccatcattta 150240

ttccatctgc accaaagaag cgtaggcccg ccatgcagcg taaaaaggat aatgcggagc 150300

agcggtcccc aaagactgat ttatcaaaac tgttttcgca gcaattaaca cacccaccga 150360

ccagtaacac cgctgcaagc aaggccacgg gcaccgccga gcgcaagggc aagcaacccg 150420

tacgccgcgc agttcagcaa gaagaaaccg aacagcagcc ccagactatg gaacaaccaa 150480

ctatgctcgc gaatagccct cgcgagtcca ctcaatatca acacccatca tcagatgaca 150540

ccatagattt agtctcctgc gaacctcctc cagcgtcaaa taacgcattt taccagcgcc 150600

cccgtagggg tagggggaga cggccatcgg cgatgaaaag agtgtcatca tctagcgccg 150660

gagctgagtc ttgtatgtca ataggcgtcc cagaaatgcc tactatgaac ggacctttgc 150720

taatgacaaa tggggatcca tggcccgggt ctgagccacc gccacctggc aaggtgcggt 150780

atggcggtac tggggactat cgccaggggc tgtgggatga accagagata cggtatgcca 150840

gagagcggta tgccgagagc cgcggtccgg tggctgtttt tgtgggagag atgggggatt 150900

catcaaagca gtatgatgct cttgtaaaag cactatatag caatagtttc gatgctatgg 150960

gctggataca atgtaacaaa ataactggtg aggatctgtt attggagcaa gtttgtcaaa 151020

aacgatgcaa tctgaggggt ggtactggtt ccgggtcatt tattactgga agtgttgcgc 151080

gacctctacc acatattgga gatgcgatgg cggctggaaa tgctctatgg ggtctaccgc 151140

atgttgccgc ggccgtagcg atgagccgcc gctatgataa agtgcaaaaa caatttctgc 151200

aaatgtctct gcggagggcg tatgcttaca tgcttttccc cgcaaaggcc gttgatgcct 151260

ccgcatctct aacggatgac atttcttcca tttatcgccg aatgaatgct gtccgccgca 151320

gcatgcgcga agctaccgag ggtctgcaga ctgtgacggt tcgcggcgac tcgcagatag 151380

agccgcagaa cacaccgcag tccacggacc atctggcgac agagcagagc gcggcggacc 151440

ccgacttttg tgatcccgaa catgcatctg attactcaac cgaggaactg gcagatgcgt 151500

gcgttttagc ttgtaatgca gtaatagagg tattgctcga gtgctttggt ggtgccggca 151560

acattcctgg tatttcctca ctggagctgg acgctgcctg tccgtctgcc cagctccacc 151620

ggcatcacgt ccagcttcta tcatggataa aggaccttaa actgttgcct atatgtataa 151680

atgctatgag actacggggc gagctttcat cgtcgcaggg cattgaatca tgtgttatgg 151740

cgcttcgtgc tgttggtacg gtgattagca ctgtgagacc ctttgtaaac ttttccgtgg 151800

acagggctag gcgtagatat tctgcatgga acctaacaga ggcgctcttt tctgccccga 151860

gcggaaatgg gtcagccaag atcaatggcg ccaaactatc ctgggtgtta agtgatgata 151920

ctgccccaaa ggaccgagat agaccagatg gcttgccaaa acatcagaat atacaaaatt 151980

ctaaaaagga taaccaaacc gggttaccgc ggacactgcc gtcggtcaag gctctcggtc 152040

caatgccgca aggcgggccc catccccgcg ggggatatag gcgcgtcccc accggaataa 152100

cacatactcc gctgccaacc gagtcggcac ggtctaccta ttgccagccc gacatagtcg 152160

cagaattatt agatgaccct atgtttcctc ctaactggaa acccgcttta atgtttgatc 152220

cggaggcact ggcccgcgtg gcggcacgac gtggcggaag ccatgtcgaa ggaagactat 152280

acgggcccct gcgagtcact gatccgctcc gcaaacaatg tgcttggatg aaccagattc 152340

cagaccctga ggatgtaaag gtggtcatca tgtactgccc gctcgtaggg gaggaattaa 152400

gcgccgcccc agtggcctct tgcatgccta tgtggtctga tgaaaaaggt ggcctctcct 152460

tcctgctagc cgccttgtgt gaccgccttt gttcatacgg cagtcacgca tgggcgggta 152520

actggactgg ggtcccagat atttctgcac tcaattccag gggcgtgctg ctgctatccg 152580

ttagagattt aggatttgcg ggggcggttg aatatctatg cctcaggctc ggctcggccc 152640

acaagcggct cattttcctg gatacaattg atacagtgga ttggcccgcc gacggtccag 152700

caattagtag ctatcattca tatataaagg cgactataac tccccaggcc catggggccc 152760

acagatggcc agattgtgaa gccatgtcaa gggtagtgat gacatcacgt gggatagtgg 152820

ggcctggagt ctttgcgcgg gtcgaagccg cgttcgctca gctataccct aatgaacccc 152880

cattacagct gatgagaggt gataatgtta aatatgttgt cgagacaaag gccggtcgca 152940

ctactcgcgt gccaatgacc ccccgtgaat atcgcggcct tgttcttccg caatacgacc 153000

gcaaggatat ggccttccag gcagatggac tcagtataag cggaccagat tttctagaag 153060

gggctgctta taatcatagg gcagcaaata gatggggtct aggagcacct ctgaggcctt 153120

tgtacctgac agagagccgg aagcatcctt ttgagacccc agatgacatt ccagaagtag 153180

tccgctcata ctgtgcagct gctttactcg aaccgcaaat ggagggtaat gtatgtgtgg 153240

tcaatccaaa tactatccag cgcggccaac gtccacctac aataacctgg gaactatgtc 153300

acggggggtc tagggctact agcatcgagg ccggagatat gaagcaggct attatcccgc 153360

ccaatgaaca atctaatgag gatgatgtta caattactgc cgtacagcca tctataactc 153420

caatgcccaa agcaccacat aagataaaaa ttgagatctt gtccgatggg gatgattcgg 153480

gggatgaggg caatccgtac gcgggagctt gtcgtgactt tttctgattt tttgttaaga 153540

gatttttttt acctgcaccg tattatatat tctccatttt tttagacagc cttttataca 153600

cagaaaatag cttcgctatg tatccagttt ttaaagaaat agaggtcttc gacatattca 153660

gctcgatagt taagaataaa gtctttttaa taaatgcaat ctgtcttgta gttcttattt 153720

aaaagtgacg ggcgggaata tggccgggcc aaaaagttat tggtggaatt ggtaggttgt 153780

ttggagattg catggatggt tattagatgt atagaccacc gcagctgtct ccaaaccatc 153840

caccccatcc caataagtgt caaaagtcta aatactatgt aagtccgtaa aggaatatta 153900

gttacaaaga tcactaaacc gccgaagttc atgggagact atcatggctg tcgggcaact 153960

accgctttat atttcgtttg taccaagcgg agaggctggc cgacattcat gatagtaatc 154020

cacaggtcct aattccttaa cacacatagg ctgcaattga cgcaaaaaga gggaggtaaa 154080

ataaagtgat aacagagctc tgataaagat aacgccatta caggcatcta attggattag 154140

ggttagctcc aactcctccc aacgccccca gtcctaataa ctatgctacc cggctcagta 154200

gtacctactt ggataaaggc caggtagaat ctggctacag tcaccatcta ctcggcgtat 154260

ggccaaccct agccctatta gcgcctcggc tcaatatttc ctacttgggc gaatgccaac 154320

cctaaatcag atgcagtcgc cgcctatttg gtgtattgcc aatcctttgc cgagttctcg 154380

gatgaagacc cgccccgctt gccatggggc caatcctaag agcgaactac cttcggagtc 154440

ccgtctcccc aaccctatcc aataacatta cttggcttaa ggccagccct aaccctaaga 154500

cagttctgcc tttggaacct tgccaaccct acttggctta gggccaacac tatccaataa 154560

cattacttgg cttaaggcca gccctaaccc taagacagtt ctgcctttgg aacactgcca 154620

accctaacca taataccgta aaacctttgg aacccggcca accataaccc taataccgta 154680

caacc 154685

<210> 2

<211> 711

<212> DNA

<213> Artificial sequence

<220>

<223> UL1 sequence

<400> 2

ctatcgtcgg ccatggtgtc cgggttcact tttgtcatgt cttcggccat atctcgagtc 60

tggatcgaat gcataacgga tgctggcctg agggtctttg aagcgatgca tattcgcgtt 120

tccgtggcag gttctgtcat ttgggtctcc ctcgttgaca cagccagtac ctacaggcgc 180

agtactaaag ctcggcttcc cgccccacgc tgaatttaag gctttctgaa aagccttgac 240

ccccacgtca tcacctattg caacttcaga gtccagcaaa aacccagaaa tcgcaaggtt 300

agggttgacc cataatgccc gctgtggacg ctcataccac aaaactacct caatttgggg 360

acagtgactg cggataaata ctccagacaa caactccgtg ctctttggtg gtacagctgc 420

ataggttgaa gtctccgagg tccctgggcg gaggcagtcc ctagatagta tagacaggac 480

atctggagca tacataactc cttcgataac cgggtaacta caggaagttt cttcaaaaaa 540

acccattacg actaggatca cgagcgccaa tctatgtaaa gtccaaccca ttgtcccgca 600

ccacaagttc ggaacgtttc gttcttcggc caccggacgc actacgtgta taacacgatg 660

ccgtattata cctacaagtg ttttcaagac ggccttccat ctggaaccca t 711

<210> 3

<211> 587

<212> PRT

<213> Artificial sequence

<220>

<223> amino acid sequence of VP2 protein

<400> 3

Thr Ala Pro Ala Lys Lys Asn Thr Gly Lys Leu Thr Asp His Tyr Pro

1 5 10 15

Val Val Lys Lys Pro Lys Leu Thr Glu Glu Val Ser Ala Gly Gly Gly

20 25 30

Ser Ser Ala Val Gln Asp Gly Gly Ala Thr Ala Glu Gly Thr Glu Pro

35 40 45

Val Ala Ala Ser Glu Met Ala Glu Gly Gly Gly Gly Ala Leu Gly Asp

50 55 60

Ala Ser Gly Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asn Trp His

65 70 75 80

Cys Asp Ser Gln Trp Met Gly Asn Thr Val Ile Thr Lys Thr Thr Arg

85 90 95

Thr Trp Val Leu Pro Ser Tyr Asn Asn His Ile Tyr Lys Ala Ile Thr

100 105 110

Ser Gly Thr Ser Gln Asp Ala Asn Val Gln Tyr Ala Gly Tyr Ser Thr

115 120 125

Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Cys His Phe Ser Pro

130 135 140

Arg Asp Trp Gln Arg Leu Ile Asn Asn His Trp Gly Ile Arg Pro Lys

145 150 155 160

Ser Leu Lys Phe Lys Ile Phe Asn Val Gln Val Lys Glu Val Thr Thr

165 170 175

Gln Asp Gln Thr Lys Thr Ile Ala Asn Asn Leu Thr Ser Thr Ile Gln

180 185 190

Val Phe Thr Asp Asp Glu His Gln Leu Pro Tyr Val Leu Gly Ser Ala

195 200 205

Thr Glu Gly Thr Met Pro Pro Phe Pro Ser Asp Val Tyr Ala Leu Pro

210 215 220

Gln Tyr Gly Tyr Cys Thr Met His Thr Asn Gln Asn Gly Ala Arg Phe

225 230 235 240

Asn Asp Arg Ser Ala Phe Tyr Cys Leu Glu Tyr Phe Pro Ser Gln Met

245 250 255

Leu Arg Thr Gly Asn Asn Phe Glu Phe Thr Phe Asp Phe Glu Glu Val

260 265 270

Pro Phe His Ser Met Phe Ala His Ser Gln Asp Leu Asp Arg Leu Met

275 280 285

Asn Pro Leu Val Asp Gln Tyr Leu Trp Asn Phe Asn Glu Val Asp Ser

290 295 300

Ser Arg Asn Ala Gln Phe Lys Lys Ala Val Lys Gly Ala Tyr Gly Thr

305 310 315 320

Met Gly Arg Asn Trp Leu Pro Gly Pro Lys Phe Leu Asp Gln Arg Val

325 330 335

Arg Ala Tyr Thr Gly Gly Thr Asp Asn Tyr Ala Asn Trp Asn Ile Trp

340 345 350

Ser Asn Gly Asn Lys Val Asn Leu Lys Asp Arg Gln Tyr Leu Leu Gln

355 360 365

Pro Gly Pro Val Ser Ala Thr His Thr Glu Ala Glu Ala Ser Ser Ile

370 375 380

Pro Ala Gln Asn Ile Leu Gly Leu Ala Lys Asp Pro Tyr Arg Ser Gly

385 390 395 400

Ser Thr Thr Ala Gly Ile Ser Asp Val Met Val Thr Asp Glu Gln Glu

405 410 415

Val Ala Pro Thr Asn Gly Val Gly Trp Lys Pro Tyr Gly Lys Thr Val

420 425 430

Thr Asn Glu Gln Asn Thr Thr Thr Ala Pro Thr Ser Ser Asp Leu Asp

435 440 445

Val Leu Gly Ala Leu Pro Gly Met Val Trp Gln Asn Arg Asp Ile Tyr

450 455 460

Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro Lys Thr Asp Gly Lys Phe

465 470 475 480

His Pro Ser Pro Asn Leu Gly Gly Phe Gly Leu His Asn Pro Pro Pro

485 490 495

Gln Val Phe Ile Lys Asn Thr Pro Val Pro Ala Asp Pro Pro Val Glu

500 505 510

Tyr Val His Gln Lys Trp Asn Ser Tyr Ile Thr Gln Tyr Ser Thr Gly

515 520 525

Gln Cys Thr Val Glu Met Val Trp Glu Leu Arg Lys Glu Asn Ser Lys

530 535 540

Arg Trp Asn Pro Glu Ile Gln Phe Thr Ser Asn Phe Ser Asp Arg Thr

545 550 555 560

Ser Ile Met Phe Ala Pro Asn Glu Thr Gly Gly Tyr Ile Glu Asp Arg

565 570 575

Leu Ile Gly Thr Arg Tyr Leu Thr Gln Asn Leu

580 585

<210> 4

<211> 20

<212> DNA

<213> Artificial sequence

<220>

<223> DEV-F primer

<400> 4

atgggttcca gatggaaggc 20

<210> 5

<211> 20

<212> DNA

<213> Artificial sequence

<220>

<223> DEV-R primer

<400> 5

ctatcgtcgg ccatggtgta 20

Claims

1. A duck herpesvirus type 1 (Anatid herpesvirus 1) virus strain belonging to the family of herpesviridae, subfamily alphaherpesviridae and genus Marek virus, wherein said virus strain has the genomic sequence shown in SEQ ID NO. 1 or a genomic sequence with at least 90% identity to the sequence shown in SEQ ID NO. 1.

2. The virus strain according to claim 1, wherein the genome of said virus strain comprises the sequence UL1 as shown in SEQ ID No. 2, preferably said virus strain is DEV SD70 with the accession number CGMCC 45056.

3. The viral strain according to claim 2, wherein the genomic sequence of 154685bp of the viral strain DEV SD70 has the following mutations with respect to the genomic sequence of 160663bp of the viral strain DEV SD 04:

2311 deletion of nucleotide 5373 (3063nt),

123708 together with the deletion of nucleotide 123907 (200nt),

126731 and 127138 (408nt) nucleotide deletion,

157484-deletion of nucleotide at position 157891 (408nt),

131060-

153561 153562 insert of nucleotide (104 nt).

4. A vaccine for preventing or treating avian viral infection comprises a therapeutically effective amount of the virus strain DEV SD70 having a accession number of CGMCC45056,

preferably wherein the avian viral infection is a disease caused by duck plague virus, more preferably the avian viral infection is duck viral enteritis,

preferably, wherein said virus strain DEV SD70 is a live, attenuated live or inactivated virus strain, more preferably a live virus strain,

preferably, wherein said viral strain DEV SD70 in said vaccine is at 1X 10²TCID₅₀/ml or TCID₅₀G to 1X 10⁸TCID₅₀/ml or TCID₅₀Per g, preferably 5X 10⁴TCID₅₀/ml or TCID₅₀G to 2X 10⁶TCID₅₀/ml or TCID₅₀The concentration in/g is present.

5. The vaccine of claim 4, wherein the vaccine further comprises an adjuvant.

6. Use of the virus strain DEV SD70 having accession number CGMCC45056 for the manufacture of a medicament for the prevention or treatment of an avian viral infection comprising administering to a subject in need thereof a vaccine comprising a therapeutically effective amount of the virus strain DEV SD70,

preferably wherein the subject comprises a chicken, duck, goose, pigeon, quail or ostrich, more preferably the subject is a duck,

preferably wherein the avian viral infection is a disease caused by duck plague virus, more preferably duck viral enteritis,

preferably, wherein for each subject, at 1 × 10²TCID₅₀To 1X 10⁸TCID₅₀The strain DEV SD 70/times, preferably 5X 10⁴TCID₅₀To 2X 10⁶TCID₅₀The vaccine was administered at doses of the strain DEV SD 70/time.

7. The use according to claim 6, wherein the vaccine is administered to the subject via a subcutaneous, intradermal, intramuscular, intravenous, oral or inhalation route, preferably via subcutaneous administration,

preferably, wherein the vaccine is administered to the subject at least 1 time, preferably 2 times at intervals of 60 days.

8. A recombinant virus which is an engineered virus based on a virus strain according to any one of claims 1 to 3, preferably the virus strain DEV SD70,

wherein the recombinant virus comprises a foreign gene encoding a protective antigen fragment of a waterfowl virus, the foreign gene being located in a region of the recombinant virus corresponding to a non-coding region between UL27 and UL26 of the virus strain,

preferably, the protective antigen fragment is goose pestivirus VP2 protein, duck circovirus Cap protein, goose astrovirus Cap protein, avian influenza virus HA protein, avian influenza virus NA protein, Tembusu virus E protein and Tembusu virus prM protein, more preferably goose pestivirus VP2 protein,

preferably, the protective antigen fragment is the gosling plague virus VP2 protein having the sequence defined by SEQ ID No. 3,

preferably, wherein the recombinant virus is for use in the prevention or treatment of at least one of chicken pest disease, duck pest disease and goose pest disease.

9. The recombinant virus of claim 8, wherein the recombinant virus comprises a nucleic acid encoding a CMV promoter, a foreign gene encoding a protective antigen fragment of a waterfowl virus, and a nucleic acid encoding a bGH poly adenylation signal.

10. A pharmaceutical composition comprising the recombinant virus of claim 8 or 9 and a pharmaceutically acceptable carrier.