CN114480125A - Kit for biological cell culture - Google Patents
Kit for biological cell culture Download PDFInfo
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- CN114480125A CN114480125A CN202210147470.5A CN202210147470A CN114480125A CN 114480125 A CN114480125 A CN 114480125A CN 202210147470 A CN202210147470 A CN 202210147470A CN 114480125 A CN114480125 A CN 114480125A
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- culture dish
- box body
- box
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- 238000004113 cell culture Methods 0.000 title claims abstract description 37
- 235000015097 nutrients Nutrition 0.000 claims abstract description 36
- 230000009471 action Effects 0.000 claims abstract description 27
- 238000000034 method Methods 0.000 claims description 20
- 238000007789 sealing Methods 0.000 claims description 15
- 230000008569 process Effects 0.000 claims description 13
- 239000003153 chemical reaction reagent Substances 0.000 claims description 10
- 238000012258 culturing Methods 0.000 claims description 7
- 241000252254 Catostomidae Species 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 3
- 230000000644 propagated effect Effects 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 29
- 238000001125 extrusion Methods 0.000 abstract description 5
- 210000004027 cell Anatomy 0.000 description 47
- 239000000428 dust Substances 0.000 description 7
- 230000005611 electricity Effects 0.000 description 6
- 230000003068 static effect Effects 0.000 description 6
- 229920000742 Cotton Polymers 0.000 description 4
- 230000000903 blocking effect Effects 0.000 description 4
- 238000001179 sorption measurement Methods 0.000 description 4
- 230000005484 gravity Effects 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 101150038956 cup-4 gene Proteins 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 241000700605 Viruses Species 0.000 description 1
- 238000010370 cell cloning Methods 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000003640 drug residue Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
Abstract
The invention relates to the technical field of kits, in particular to a kit for biological cell culture, which comprises a culture dish, a box body and a controller, wherein the culture dish is arranged on the box body; the culture dish is positioned in the box body and comprises an upper end cover and a lower end cover; the upper end of the box body is provided with an opening, the upper end of the box body is hinged with a box cover, and a round box is arranged inside the box body; the lower end of the round box is fixedly connected to the bottom of the box body through a support leg, and the interior of the round box is rotatably connected with a swash plate; one surface of the swash plate, which is far away from the support legs, is obliquely arranged; a motor is arranged between the round box and the bottom of the box body; the motor is fixedly connected to the bottom of the box body, and an output shaft of the motor extends into the round box; the motor drives the swash plate to rotate, and the swash plate and the slide bar drive the culture dish to shake under the extrusion action of the swash plate, so that biological cells and nutrient solution in the culture dish are dispersed under the shaking action, and the condition that the biological cells are unevenly distributed in the nutrient solution to influence the biological culture effect is prevented.
Description
Technical Field
The invention relates to the technical field of kits, in particular to a kit for biological cell culture.
Background
Cell culture refers to a method for simulating in vivo environment in vitro, and enabling the in vivo environment to survive, grow, reproduce and maintain the main structure and function under sterile conditions, suitable temperature, pH value, certain nutritional conditions and the like, and is also called cell cloning technology, and the formal term in biology is cell culture technology; the kit is used for containing a box for detecting chemical reagents such as chemical components, drug residues, virus types and the like; biological cell and nutrient solution are stored in the culture dish, and the culture dish is deposited in the reagent box, and in carrying out biological cell culture process, the condition of piling can appear in the biological cell to make biological cell distribute inhomogeneous in the nutrient solution, and then influence biological cell culture's effect.
Some technical schemes related to a biological cell culture kit also appear in the prior art, for example, a chinese patent with application number CN201920902155.2 discloses a biological cell culture kit, which includes a base, a fixing seat is arranged in the base, a driving motor is embedded in the fixing seat, an output shaft of the driving motor is connected to a culture dish, a dustproof cover plate is arranged on the upper surface of the base, the dustproof cover plate is fixedly mounted on the base through a cutting, a reagent adding mechanism is arranged on the dustproof cover plate, and the reagent adding mechanism includes a dropping pipe, a tee joint, a first drainage pipe, a second drainage pipe and a limiting plate; according to the kit for biological cell culture, in the process of adding the reagent, the dustproof cover plate does not need to be taken down, so that the culture dish can be prevented from being polluted by the external environment, the quality of cultured cells can be improved, impurities such as dust can be prevented from entering the dropping pipe through the dustproof sealing cover to pollute a reagent adding mechanism, the dustproof sealing cover can be conveniently taken through the connecting rope, the situation that the dustproof sealing cover cannot be found due to random placement is avoided, the finding time of an operator is prolonged, and the cell culture efficiency is low; however, in the process of culturing biological cells, the biological cells are packed, so that the distribution of the biological cells in the nutrient solution is uneven, the effect of culturing the biological cells is affected, and the limitation of the technical scheme is caused.
In view of the above, in order to overcome the above technical problems, the present invention provides a kit for culturing biological cells, which solves the above technical problems.
Disclosure of Invention
In order to make up for the defects of the prior art, the reagent kit for culturing the biological cells, which is provided by the invention, drives the swash plate to rotate through the motor, and then is matched with the culture dish which is driven by the slide rod under the extrusion action of the swash plate to shake, so that the biological cells and the nutrient solution in the culture dish are dispersed under the shaking action, and the condition that the biological cells are unevenly distributed in the nutrient solution to influence the biological culture effect is prevented.
The technical scheme adopted by the invention for solving the technical problems is as follows: the invention relates to a kit for biological cell culture, which comprises a culture dish, a box body and a controller; the culture dish is positioned in the box body and comprises an upper end cover and a lower end cover; the biological cell culture method of the kit comprises the following steps:
s1, the staff firstly carries out disinfection and cleaning treatment on the upper end cover and the lower end cover, then debugs each element in the box body, and finally checks the connection condition of each element of the kit; the sterilization and cleaning treatment is carried out, so that the influence of bacteria inside the upper end cover and the lower end cover on the biological cell culture is prevented; the stability of the kit in operation is improved by debugging and checking each element;
s2, the worker preheats the culture dish to 15-25 ℃, then pours the biological cells and the nutrient solution into the lower end cover, and finally clamps the upper end cover on the lower end cover; the culture dish is preheated, so that the condition that the temperature of the culture dish is lower than 15 ℃ to influence the activity of biological cells is prevented, and the biological cell culture effect is improved;
s3, the worker places the culture dish in the box body until the bottom surface of the culture dish is sucked by the sucker, the controller is started to control the motor to rotate periodically, the rotating motor drives the fixedly connected swash plate to rotate, so that the slide bar is driven by the swash plate to slide along the upper part of the round box, the culture dish is driven by the sucker to swing in the motion process of the slide bar, and biological cells can be propagated and grown in the culture dish; the shaking of the culture dish can drive the biological cells and the nutrient solution inside to shake, so that the biological cells and the nutrient solution are distributed in the culture dish in a discrete way;
the upper end of the box body is provided with an opening, the upper end of the box body is hinged with a box cover, and a round box is arranged inside the box body; the lower end of the round box is fixedly connected to the bottom of the box body through a support leg, and the interior of the round box is rotatably connected with a swash plate; one surface of the swash plate, which is far away from the support legs, is obliquely arranged; a motor is arranged between the round box and the bottom of the box body; the motor is fixedly connected to the bottom of the box body, an output shaft of the motor extends into the round box, and the output shaft of the motor is fixedly connected with the swash plate; the upper part of the round box is connected with a slide bar in a sliding way; one end of the sliding rod, which is positioned at the round box, is fixedly connected with a ball, and the other end of the sliding rod is hinged with a sucking disc; the controller is used for controlling the automatic operation of the biological cell culture kit;
when the biological cell culture device works, biological cells and nutrient solution are stored in the culture dish, the culture dish is stored in the reagent box, and the biological cells are bundled in the biological cell culture process, so that the biological cells are unevenly distributed in the nutrient solution, and the biological cell culture effect is further influenced;
according to the invention, after biological cells and nutrient solution are poured into the lower end cover by a worker, the upper end cover and the lower end cover are clamped to form a culture dish, the culture dish is placed in the box body until the bottom surface of the culture dish is sucked by the sucker, the controller is started to control the motor to rotate periodically, the rotating motor drives the fixedly connected swash plate to rotate, the round ball at one end of the slide bar can be contacted with the swash plate under the gravity action of the culture dish, so that the round ball can be extruded by the rotation of the swash plate, and then the round ball drives the slide bar to slide along the upper part of the round box, so that the culture dish is driven by the sucker to shake in the motion process of the slide bar, the culture dish can drive the biological cells and the nutrient solution in the culture dish to shake, and the biological cells and the nutrient solution are distributed in the culture dish in a discrete manner;
the motor drives the swash plate to rotate, and the swash plate and the slide bar drive the culture dish to shake under the extrusion action of the swash plate, so that biological cells and nutrient solution in the culture dish are dispersed under the shaking action, and the condition that the biological cells are unevenly distributed in the nutrient solution to influence the biological culture effect is prevented.
Preferably, a push disc is arranged between the suction disc and the round box; the push disc is connected with the slide bar in a sliding manner; electric push rods are arranged around the round boxes; one end of the electric push rod is fixedly connected to the bottom of the box body, and the other end of the electric push rod is fixedly connected with the push disc; when the device works, before a culture dish is placed into the box body by a worker, the controller controls the electric push rod to extend, the electric push rod can drive the push disc to move away from the disc, the push disc slides along the slide rod and then contacts with the sucker, and the push disc drives the slide rod to move upwards under the blocking of the sucker, so that the slide rod keeps the same height after being away from the swash plate, the bottom surface of the culture dish is conveniently sucked on the sucker, and the practical application effect of the device is improved; when the sucker is sucked on the bottom surface of the culture dish, the electric push rod drives the push disc to move close to the round box until the push disc is contacted with the round box.
Preferably, the upper end of the push disc is fixedly connected with an annular sleeve; the number of the annular sleeves is consistent with that of the suckers, the annular sleeves are sleeved on the slide bars and can be contacted with the suckers under the action of the push disc; when the circular box sucking disc type culture dish sucking disc device works, the push disc can drive the annular sleeve to move in the movement process that the push disc is far away from the circular box, so that the annular sleeve is in contact with the sucking disc close to the outer wall of the circular box under the action of the push disc, the suction port of the sucking disc is ensured to be vertically upward, the culture dish is convenient to adsorb, the operation of workers is facilitated, and the practicability of the circular box sucking disc type culture dish device is improved.
Preferably, the inner wall of the annular sleeve is embedded with grinding blocks which are uniformly distributed on the inner wall of the annular sleeve, and the annular sleeve is made of flexible materials such as rubber; when the culture dish device works, after the annular sleeve is contacted with the sucker, the electric push rod drives the push disc to continuously move upwards, so that the push disc drives the annular sleeve to extrude the sucker, the annular sleeve can deform after being pressed, the annular sleeve can drive the grinding block to rub against the outer wall of the sucker while being pressed, the grinding block is subjected to a rubbing action to generate static electricity, the grinding block with the static electricity can adsorb dust on the inner wall of the sucker, the adsorption effect of the sucker on the culture dish is further prevented from being influenced by the dust, and the stability of the culture dish device is improved.
Preferably, a step hole is formed in the sucker; a spring and a sealing ball are arranged in the stepped hole; the sealing ball seals the stepped hole under the action of the spring, and a rope is fixedly connected to the outer wall of the sealing ball, which is close to the annular sleeve; the other end of the cord extends to the outside of the stepped hole, and the other end of the cord is knotted; the during operation, when the staff need take off the culture dish from the sucking disc, controller control electric putter is worked once more, electric putter drives and pushes away the dish and keeps away from the round box and remove, it drives annular cover and sucking disc contact once more to push away the dish, can follow the outer wall that the sucking disc was pasted after the annular cover pressurized and continue to move, the edge of annular cover can promote the other end of cotton rope and remove, the cotton rope removes the in-process and can drive the sealed ball and remove, thereby loosen the sealed ball from the step downthehole, make the inside and outside atmospheric pressure of sucking disc keep unanimous, the culture dish of being convenient for takes off from the sucking disc.
Preferably, one side of the swash plate, which is far away from the support legs, is provided with a strip-shaped groove; the strip-shaped grooves are uniformly distributed on one surface of the swash plate; the during operation, the motor drives the sloping cam plate and rotates the in-process, the one side and the ball contact of carousel, so the sloping cam plate forms vibrations effect with the ball under the effect in bar groove, and the ball can loop through the draw runner with the shaking force, the sucking disc transmits to the culture dish on to make biological cell and the nutrient solution in the culture dish disperse more under the effect of vibrations, and then be favorable to biological cell culture.
The invention has the following beneficial effects:
1. according to the invention, the motor drives the swash plate to rotate, and then the swash plate and the slide bar drive the culture dish to shake and match under the extrusion action of the swash plate, so that biological cells and nutrient solution in the culture dish are dispersed under the shaking action, and the condition that the biological cells are unevenly distributed in the nutrient solution to influence the biological culture effect is prevented.
2. Before the culture dish is placed into the box body by a worker, the controller controls the electric push rod to extend, the electric push rod can drive the push disc to move away from the disc, the push disc slides along the slide rod and then contacts with the sucker, and the push disc drives the slide rod to move upwards under the blocking of the sucker, so that the slide rod keeps the same height after being away from the swash plate, the bottom surface of the culture dish is conveniently sucked on the sucker, and the practical application effect of the culture dish is improved.
3. In the process that the push disc moves away from the round box, the push disc can drive the annular sleeve to move, so that the annular sleeve is in contact with the outer wall of the sucker close to the round box under the action of the push disc, the suction port of the sucker is ensured to be vertically upward, the culture dish is conveniently adsorbed, the operation of workers is facilitated, and the practicability of the culture dish is improved.
Drawings
The invention will be further explained with reference to the drawings.
FIG. 1 is a flow chart of a method of culturing biological cells according to the present invention;
FIG. 2 is a perspective view of the present invention;
FIG. 3 is a cross-sectional view of the present invention;
FIG. 4 is an enlarged view at A in FIG. 3;
FIG. 5 is a perspective view of the swash plate of the present invention;
in the figure: the culture dish comprises a culture dish 1, an upper end cover 11, a lower end cover 12, a box body 2, a box cover 21, a round box 22, support legs 23, a motor 24, a slide bar 25, a round ball 26, a swash plate 3, a strip-shaped groove 31, a sucker 4, a step hole 41, a spring 42, a sealing ball 43, a thread rope 44, a push disc 5, an electric push rod 6, an annular sleeve 7 and a grinding block 71.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
As shown in fig. 1 to 5, the kit for culturing biological cells according to the present invention includes a culture dish 1, a box 2, and a controller; the culture dish 1 is positioned in the box body 2, and the culture dish 1 comprises an upper end cover 11 and a lower end cover 12; the biological cell culture method of the kit comprises the following steps:
s1, the staff firstly carries out disinfection and cleaning treatment on the upper end cover 11 and the lower end cover 12, then debugs each element in the box body 2, and finally checks the connection condition of each element of the kit; by performing the sterilization and cleaning processes, the bacteria inside the upper end cap 11 and the lower end cap 12 are prevented from affecting the biological cell culture; the stability of the kit in operation is improved by debugging and checking each element;
s2, the worker preheats the culture dish 1 to 15-25 ℃, then pours the biological cells and the nutrient solution into the lower end cover 12, and finally clamps the upper end cover 11 on the lower end cover 12; by preheating the culture dish 1, the temperature of the culture dish 1 is prevented from being lower than 15 ℃ to influence the activity of biological cells, so that the biological cell culture effect is improved;
s3, the worker places the culture dish 1 in the box body 2 until the bottom surface of the culture dish 1 is sucked by the sucker 4, the controller is started to control the motor 24 to rotate periodically, the rotating motor 24 drives the fixedly connected swash plate 3 to rotate, so that the swash plate 3 drives the slide bar 25 to slide along the upper part of the round box 22, the culture dish 1 is driven by the sucker 4 to shake in the motion process of the slide bar 25, and biological cells can propagate and grow in the culture dish 1; the shaking of the culture dish 1 can drive the biological cells and the nutrient solution inside to shake, so that the biological cells and the nutrient solution are distributed in the culture dish 1 in a discrete manner;
an opening is formed in the upper end of the box body 2, a box cover 21 is hinged to the upper end of the box body 2, and a round box 22 is arranged inside the box body 2; the lower end of the round box 22 is fixedly connected to the bottom of the box body 2 through a support leg 23, and the interior of the round box 22 is rotatably connected with the swash plate 3; the side of the swash plate 3 far away from the support leg 23 is obliquely arranged; a motor 24 is arranged between the round box 22 and the bottom of the box body 2; the motor 24 is fixedly connected to the bottom of the box body 2, an output shaft of the motor 24 extends into the round box 22, and an output shaft of the motor 24 is fixedly connected with the swash plate 3; the upper part of the round box 22 is connected with a slide bar 25 in a sliding way; one end of the sliding rod 25, which is positioned at the round box 22, is fixedly connected with a round ball 26, and the other end of the sliding rod 25 is hinged with a sucking disc 4; the controller is used for controlling the automatic operation of the biological cell culture kit;
when the biological cell culture device works, biological cells and nutrient solution are stored in the culture dish 1, the culture dish 1 is stored in the reagent box, and the biological cells are bundled in the biological cell culture process, so that the biological cells are unevenly distributed in the nutrient solution, and the biological cell culture effect is further influenced;
therefore, in the invention, after biological cells and nutrient solution are poured into the lower end cover 12 by a worker, the upper end cover 11 and the lower end cover 12 are clamped to form the culture dish 1, the culture dish 1 is placed in the box body 2 until the bottom surface of the culture dish 1 is sucked by the sucker 4, the controller is started to control the motor 24 to periodically rotate, the rotating motor 24 drives the fixedly connected swash plate 3 to rotate, the round ball 26 at one end of the slide bar 25 is contacted with the swash plate 3 under the action of gravity of the culture dish 1, so that the round ball 26 can be extruded by the rotation of the swash plate 3, the round ball 26 drives the slide bar 25 to slide along the upper part of the round box 22, the culture dish 1 is driven by the sucker 4 to shake in the motion process of the slide bar 25, the shake of the culture dish 1 can drive the biological cells and the nutrient solution inside to shake, and the biological cells and the nutrient solution are distributed in the culture dish 1 in a discrete manner;
according to the invention, the motor 24 drives the swash plate 3 to rotate, and then the swash plate 3 drives the culture dish 1 to shake and match with the slide bar 25 under the extrusion action of the swash plate 3, so that biological cells and nutrient solution in the culture dish 1 are dispersed under the shaking action, and the condition that the biological cells are unevenly distributed in the nutrient solution to influence the biological culture effect is prevented.
As an embodiment of the invention, a push disc 5 is arranged between the suction disc 4 and the round box 22; the push disc 5 is in sliding connection with the slide bar 25; an electric push rod 6 is arranged around the round box 22; one end of the electric push rod 6 is fixedly connected to the bottom of the box body 2, and the other end of the electric push rod is fixedly connected with the push disc 5; when the culture dish lifting device works, before a worker puts a culture dish 1 into a box body 2, the controller controls the electric push rod 6 to extend, the electric push rod 6 can drive the push disc 5 to move away from the disc, the push disc 5 slides along the slide rod 25 and then contacts with the sucking disc 4, the push disc 5 drives the slide rod 25 to move upwards under the blocking of the sucking disc 4, so that the slide rod 25 keeps the same height after being away from the swash plate 3, the bottom surface of the culture dish 1 is conveniently sucked on the sucking disc 4, and the practical application effect of the culture dish lifting device is improved; when the suction cup 4 is sucked on the bottom surface of the culture dish 1, the electric push rod 6 drives the push disc 5 to move close to the round box 22 until the push disc 5 contacts with the round box 22.
As an embodiment of the invention, the upper end of the push disc 5 is fixedly connected with an annular sleeve 7; the number of the annular sleeves 7 is consistent with that of the suckers 4, the annular sleeves 7 are sleeved on the slide bar 25, and the annular sleeves 7 can be contacted with the suckers 4 under the action of the push disc 5; during operation, in the movement process that the push disc 5 is far away from the round box 22, the push disc 5 can drive the annular sleeve 7 to move, so that the annular sleeve 7 is in contact with the sucking disc 4 close to the outer wall of the round box 22 under the action of the push disc 5, the suction port of the sucking disc 4 is ensured to be vertically upward, the culture dish 1 is convenient to adsorb, the operation of workers is facilitated, and the practicability of the culture dish is improved.
As an embodiment of the present invention, the inner wall of the annular sleeve 7 is embedded with grinding blocks 71, the grinding blocks 71 are uniformly distributed on the inner wall of the annular sleeve 7, and the annular sleeve 7 is made of a flexible material, such as rubber; during operation, after the annular sleeve 7 is contacted with the sucker 4, the electric push rod 6 drives the push disc 5 to continue to move upwards, so that the push disc 5 drives the annular sleeve 7 to extrude the sucker 4, the annular sleeve 7 can deform after being pressed, the annular sleeve 7 can drive the grinding block 71 to rub against the outer wall of the sucker 4 while being pressed, the grinding block 71 generates static electricity under the action of the friction, the grinding block 71 with the static electricity can adsorb dust on the inner wall of the sucker 4, the dust is prevented from influencing the adsorption effect of the sucker 4 on the culture dish 1, and the stability of the culture dish is improved.
As an embodiment of the present invention, a step hole 41 is provided inside the suction cup 4; a spring 42 and a sealing ball 43 are arranged in the stepped hole 41; the sealing ball 43 seals the stepped hole 41 under the action of the spring 42, and a rope 44 is fixedly connected to the outer wall of the sealing ball 43 close to the annular sleeve 7; the other end of the cord 44 extends to the outside of the stepped hole 41, and the other end of the cord 44 is knotted; the during operation, when the staff need take off culture dish 1 from sucking disc 4, controller control electric putter 6 is worked once more, electric putter 6 drives and pushes away dish 5 and keeps away from the round box 22 and removes, it drives annular cover 7 and sucking disc 4 contact once more to push away dish 5, can paste the outer wall of sucking disc 4 and continue to move after annular cover 7 pressurized, the edge of annular cover 7 can promote the other end of cotton rope 44 and remove, cotton rope 44 removes the in-process and can drive sealed ball 43 and remove, thereby loosen sealed ball 43 from in the step hole 41, make the inside and outside atmospheric pressure of sucking disc 4 keep unanimously, be convenient for culture dish 1 takes off from sucking disc 4.
As an embodiment of the present invention, a strip-shaped groove 31 is provided on one side of the swash plate 3 away from the support leg 23; the strip-shaped grooves 31 are uniformly distributed on one surface of the swash plate 3; the during operation, motor 24 drives swash plate 3 and rotates the in-process, and the one side and the ball 26 contact of carousel, so swash plate 3 forms vibrations effect with ball 26 under the effect of strip groove 31, and ball 26 can loop through the vibrating force on smooth stick 25, sucking disc 4 transmit to culture dish 1 to make biological cell and nutrient solution in the culture dish 1 disperse more under the vibrations effect, and then be favorable to biological cell culture.
When the culture dish works, after biological cells and nutrient solution are poured into the lower end cover 12 by a worker, the upper end cover 11 and the lower end cover 12 are clamped to form the culture dish 1, the culture dish 1 is placed in the box body 2 until the bottom surface of the culture dish 1 is sucked by the sucker 4, the controller is started to control the motor 24 to rotate periodically, the rotating motor 24 drives the fixedly connected swash plate 3 to rotate, the round ball 26 at one end of the sliding rod 25 is contacted with the swash plate 3 under the action of gravity of the culture dish 1, so that the round ball 26 can be extruded by the rotation of the swash plate 3, the round ball 26 drives the sliding rod 25 to slide along the upper part of the round box 22, the culture dish 1 is driven by the sucker 4 to shake in the motion process of the sliding rod 25, the culture dish 1 shakes to drive the biological cells and the nutrient solution inside to shake, and the biological cells and the nutrient solution are distributed in the culture dish 1 in a discrete manner; before the culture dish 1 is placed into the box body 2 by a worker, the controller controls the electric push rod 6 to extend, the electric push rod 6 can drive the push disc 5 to move away from the disc, the push disc 5 slides along the slide rod 25 and then contacts with the sucker 4, and the push disc 5 drives the slide rod 25 to move upwards under the blocking of the sucker 4, so that the slide rod 25 keeps the same height after being away from the swash plate 3, the bottom surface of the culture dish 1 is conveniently sucked on the sucker 4, and the practical application effect of the culture dish is improved; when the sucker 4 is sucked on the bottom surface of the culture dish 1, the electric push rod 6 drives the push disc 5 to move close to the round box 22 until the push disc 5 is contacted with the round box 22, and the push disc 5 moves away from the round box 22, the push disc 5 can drive the annular sleeve 7 to move, so that the annular sleeve 7 is contacted with the outer wall of the sucker 4 close to the round box 22 under the action of the push disc 5, and further the suction port of the sucker 4 is ensured to be vertically upward, thereby facilitating the adsorption of the culture dish 1, being beneficial to the operation of workers, improving the practicability of the invention, after the annular sleeve 7 is contacted with the sucker 4, the electric push rod 6 drives the push disc 5 to continue to move upward, so that the push disc 5 drives the annular sleeve 7 to extrude the sucker 4, the annular sleeve 7 can deform after being pressed, the annular sleeve 7 can drive the grinding block 71 to generate friction with the outer wall of the sucker 4 while being pressed, thereby enabling the grinding block 71 to generate static electricity under the friction action, the grinding block 71 with static electricity can adsorb dust on the inner wall of the sucker 4, so that the adsorption effect of the sucker 4 on the culture dish 1 is prevented from being influenced by the dust, the stability of the culture dish is improved, when a worker needs to take the culture dish 1 off the sucker 4, the controller controls the electric push rod 6 to work again, the electric push rod 6 drives the push disc 5 to move away from the round box 22, the push disc 5 drives the annular sleeve 7 to contact with the sucker 4 again, the annular sleeve 7 can move continuously along the outer wall of the sucker 4 after being pressed, the edge of the annular sleeve 7 can push the other end of the thread rope 44 to move, the thread rope 44 can drive the sealing ball 43 to move in the moving process, the sealing ball 43 is loosened from the stepped hole 41, the air pressure inside and outside the sucker 4 is kept consistent, the culture dish 1 is convenient to take off from the sucker 4, the motor 24 drives the swash plate 3 to rotate, one surface of the rotary plate is in contact with the round ball 26, therefore, the swash plate 3 and the round ball 26 form a vibration effect under the action of the strip-shaped groove 31, and the round ball 26 transmits vibration force to the culture dish 1 through the slide bar 25 and the sucking disc 4 in sequence, so that biological cells and nutrient solution in the culture dish 1 are more dispersed under the vibration effect, and the biological cell culture is facilitated.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (6)
1. A reagent kit for biological cell culture comprises a culture dish (1), a box body (2) and a controller; the culture dish (1) is positioned inside the box body (2), and the culture dish (1) comprises an upper end cover (11) and a lower end cover (12); the method is characterized in that: the biological cell culture method of the kit comprises the following steps:
s1, the staff firstly carries out disinfection and cleaning treatment on the upper end cover (11) and the lower end cover (12), then debugs each element in the box body (2), and finally checks the connection condition of each element of the kit;
s2, the worker preheats the culture dish (1) to 15-25 ℃, then pours the biological cells and the nutrient solution into the lower end cover (12), and finally clamps the upper end cover (11) on the lower end cover (12);
s3, the worker places the culture dish (1) in the box body (2) until the bottom surface of the culture dish (1) is sucked by the sucker (4), the controller is started to control the motor (24) to rotate periodically, the rotating motor (24) drives the fixedly connected swash plate (3) to rotate, so that the swash plate (3) drives the slide bar (25) to slide along the upper part of the round box (22), the sucker (4) drives the culture dish (1) to shake in the moving process of the slide bar (25), and biological cells can be propagated and grown in the culture dish (1);
an opening is formed in the upper end of the box body (2), a box cover (21) is hinged to the upper end of the box body (2), and a round box (22) is arranged inside the box body (2); the lower end of the round box (22) is fixedly connected to the bottom of the box body (2) through a support leg (23), and the interior of the round box (22) is rotatably connected with the swash plate (3); one surface of the swash plate (3) far away from the support leg (23) is obliquely arranged; a motor (24) is arranged between the round box (22) and the bottom of the box body (2); the motor (24) is fixedly connected to the bottom of the box body (2), an output shaft of the motor (24) extends into the round box (22), and an output shaft of the motor (24) is fixedly connected with the swash plate (3); the upper part of the round box (22) is connected with a slide bar (25) in a sliding way; one end of the sliding rod (25) positioned on the round box (22) is fixedly connected with a round ball (26), and the other end of the sliding rod (25) is hinged with a sucking disc (4); the controller is used for controlling the automatic operation of the biological cell culture kit.
2. The biological cell culture kit according to claim 1, wherein: a push disc (5) is arranged between the suction disc (4) and the round box (22); the push disc (5) is in sliding connection with the slide bar (25); an electric push rod (6) is arranged around the round box (22); one end of the electric push rod (6) is fixedly connected with the bottom of the box body (2), and the other end is fixedly connected with the push disc (5).
3. The biological cell culture kit according to claim 2, wherein: the upper end of the push disc (5) is fixedly connected with an annular sleeve (7); the number of the annular sleeves (7) is consistent with that of the suckers (4), the annular sleeves (7) are sleeved on the slide rods (25), and the annular sleeves (7) can be in contact with the suckers (4) under the action of the push disc (5).
4. The biological cell culture kit according to claim 3, wherein: the inner wall of the annular sleeve (7) is embedded with grinding blocks (71), the grinding blocks (71) are uniformly distributed on the inner wall of the annular sleeve (7), and the annular sleeve (7) is made of flexible materials.
5. The kit for culturing biological cells according to claim 4, wherein: a step hole (41) is formed in the sucker (4); a spring (42) and a sealing ball (43) are arranged in the stepped hole (41); the sealing ball (43) seals the stepped hole (41) under the action of the spring (42), and a thread rope (44) is fixedly connected to the outer wall, close to the annular sleeve (7), of the sealing ball (43); the other end of the thread rope (44) extends to the outside of the stepped hole (41), and the other end of the thread rope (44) is knotted.
6. The biological cell culture kit according to claim 5, wherein: a strip-shaped groove (31) is formed in one surface, far away from the supporting leg (23), of the swash plate (3); the strip-shaped grooves (31) are uniformly distributed on one surface of the swash plate (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210147470.5A CN114480125A (en) | 2022-02-17 | 2022-02-17 | Kit for biological cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210147470.5A CN114480125A (en) | 2022-02-17 | 2022-02-17 | Kit for biological cell culture |
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| Publication Number | Publication Date |
|---|---|
| CN114480125A true CN114480125A (en) | 2022-05-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210147470.5A Withdrawn CN114480125A (en) | 2022-02-17 | 2022-02-17 | Kit for biological cell culture |
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| Country | Link |
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| CN (1) | CN114480125A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116426385A (en) * | 2023-06-13 | 2023-07-14 | 北京大学深圳医院（北京大学深圳临床医学院） | Cell culture apparatus and cell culture method |
-
2022
- 2022-02-17 CN CN202210147470.5A patent/CN114480125A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116426385A (en) * | 2023-06-13 | 2023-07-14 | 北京大学深圳医院（北京大学深圳临床医学院） | Cell culture apparatus and cell culture method |
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Application publication date: 20220513 |