JP2010273603A - Automated culture apparatus, working table and incubator - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an automated culture apparatus which represses contamination of foreign substances to a culture vessel, a working table and an incubator. <P>SOLUTION: In the automated culture apparatus 100, a movable part 31<SB>MOVE</SB>, movable part 32<SB>MOVE</SB>, movable part 41<SB>MOVE</SB>and movable part 42<SB>MOVE</SB>are each arranged below the reference position SP set above a placing surface 20S. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

  The present invention relates to an automatic culture apparatus, work table, and incubator used for cell culture.

  Conventionally, cell culture has been widely performed for the purpose of cell proliferation and differentiation. Specifically, the cell culture includes a step of culturing the cells in a medium, a step of washing the cells with PBS (Phosphate Buffered Salts), a step of detaching the cells from the culture vessel with trypsin, or a step of adding various reagents. Is provided. These processes are extremely complicated and time-consuming operations when culturing a large amount of cells. In addition, since the efficiency of such work is easily influenced by the skill level of the worker, human error is likely to occur and productivity is easily reduced.

  Then, the automatic culture apparatus for performing cell culture automatically is proposed (refer patent document 1). Specifically, a step of supplying and discarding the culture medium to the culture vessel, a step of supplying trypsin to the culture vessel, and the like are performed by a pipette device having a pipette inserted into the culture vessel.

JP 2008-54690 A

  However, in the pipette device described in Patent Document 1, a drive unit that moves the pipette or a movable portion of the pipette is disposed above the culture vessel. For this reason, foreign matter such as abrasion powder generated from the drive unit or the movable unit may be mixed in the culture vessel. In the medical field such as regenerative medicine and cell therapy, the cultured cells are transplanted into the human body. Therefore, contamination of the cultured cells with bacteria and foreign substances should be avoided as much as possible.

  The present invention has been made in view of the above-described problems, and an object thereof is to provide an automatic culture apparatus, a work table, and an incubator capable of suppressing foreign matters from being mixed into a culture container.

  An automatic culture apparatus according to a feature of the present invention is an automatic culture apparatus that automatically performs a culture operation in a culture container in which cell culture is performed, and a mounting table having a mounting surface for mounting the culture container; A culture tool that is arranged on a mounting table and is used for cell culture, and a drive unit that moves the culture tool. The drive unit has a movable part that moves together with the culture tool, and the movable part is located above the placement surface. It is arranged below the set reference position, and the gist is that the reference position is set based on the position in the vertical direction of the opening provided in the culture vessel.

  According to the automatic culture apparatus according to the feature of the present invention, the wear powder generated by the friction between the components in each movable part by setting the reference position based on the position in the vertical direction of the opening provided in the culture vessel. It can suppress that foreign materials, such as, mix with a culture container. In this case, when the reference position is the same as the opening or below the opening, it is possible to effectively suppress the mixing of foreign matters.

  In the automatic culture apparatus according to the feature of the present invention, the culture tool is a cylindrical member, and is fixed to a drive unit that supplies the fluid to the culture tool and sucks the fluid from the culture tool, A support unit that supports the culture tool, and the drive unit may move the support unit in the vertical direction. According to this, the supply or suction of the fluid to the culture container can be performed while suppressing foreign matter from being mixed into the culture container.

  In the automatic culture apparatus according to the features of the present invention, the tip of the culture tool may be a scraper or a roller cutter, and the drive unit may move the tip in the vertical direction and the horizontal direction. According to this, the operation | work which peels a cell group from a culture surface, the operation | work which divides | segments a cultured cell on a culture surface, etc. can be performed, suppressing that a foreign material mixes into a culture container.

  In the automatic culture apparatus according to the features of the present invention, the culture tool may have a plurality of gripping units, and the drive unit may move each of the plurality of gripping units in the vertical direction and the horizontal direction. According to this, various tools can be gripped and conveyed into the culture container while suppressing foreign matters from being mixed into the culture container.

  A work table according to the features of the present invention includes a work room surrounded by walls, an automatic culture apparatus that is placed outside the work room and automatically performs a culture operation in a culture vessel in which cell culture is performed, and a work room. A door provided on the wall between the automatic culture apparatus, the automatic culture apparatus having a mounting surface for mounting a culture vessel, and a cell culture medium disposed above the mounting table. And a drive unit that moves the culture tool. The drive unit has a movable part that moves together with the culture tool, and the movable part is below the reference position set above the placement surface. The gist is that the reference position is set based on the position in the vertical direction of the opening provided in the culture vessel.

  According to the workbench according to the features of the present invention, it is possible to ensure sterility in the work chamber, effectively utilize the work space, and suppress human errors such as turning over the culture container, and culture processed in the work chamber. The container can be easily carried into the automatic culture apparatus.

  An incubator according to a feature of the present invention includes a culture chamber having a plurality of storage units, an automatic culture device that automatically performs a culture operation in a culture vessel in which cell culture is performed, and a temperature control device that adjusts the temperature in the culture chamber. An automatic transfer device for transferring culture containers between the plurality of storage units and the automatic culture apparatus, the automatic culture apparatus comprising: a mounting table having a mounting surface for mounting the culture container; It has a culture tool used for cell culture and a drive unit that moves the culture tool. The drive unit has a movable part that moves with the culture tool, and the movable part is set above the mounting surface. The gist is that the reference position is set based on the position in the vertical direction of the opening provided in the culture vessel.

  According to the incubator according to the features of the present invention, it is possible to ensure sterility in the culture chamber, effectively use the work space, and suppress human errors such as turning over the culture vessel. Both treatment to the culture container and cell culture in the culture container can be automatically performed.

  ADVANTAGE OF THE INVENTION According to this invention, the automatic culture apparatus, worktable, and incubator which can suppress mixing of the foreign material to a culture container can be provided.

It is a side view which shows the structure of the automatic culture apparatus 100 which concerns on 1st Embodiment of this invention. It is a flowchart for demonstrating schematic operation | movement of the cell culture method which concerns on 1st Embodiment of this invention. It is a flowchart for demonstrating the detail of a subculture process. It is an external view which shows the structure of the cell processing isolator 200 which concerns on 2nd Embodiment of this invention. It is an external view which shows the structure of the safety cabinet 300 which concerns on 2nd Embodiment of this invention. It is an external view which shows the structure of the incubator 400 which concerns on 2nd Embodiment of this invention. It is a figure which shows the structure of the automatic culture apparatus 100 provided with the culture tool 50 which concerns on embodiment of this invention.

  Next, embodiments of the present invention will be described with reference to the drawings. In the following description of the drawings, the same or similar parts are denoted by the same or similar reference numerals. However, it should be noted that the drawings are schematic and ratios of dimensions and the like are different from actual ones. Accordingly, specific dimensions and the like should be determined in consideration of the following description. Moreover, it is a matter of course that portions having different dimensional relationships and ratios are included between the drawings.

[First Embodiment]
(Configuration of automatic culture equipment)
The configuration of the automatic culture apparatus according to the first embodiment will be described with reference to FIG. FIG. 1 is a side view showing a configuration of an automatic culture apparatus 100 according to the first embodiment.

The automatic culture apparatus 100 is an apparatus that automatically performs a culture operation in a culture vessel C in which cell culture is performed. As shown in FIG. 1, the automatic culture apparatus 100 includes a substrate 10, a mounting table 20, a first culture tool 30, a first vertical drive unit 31, a first vertical motor M ₃₁ , a first horizontal drive unit 32, and a first horizontal. A motor M ₃₂ , a support unit 33, a liquid feeding device 34, a second culture tool 40, a second vertical drive unit 41, a second vertical motor M ₄₁ , a second horizontal drive unit 42, and a second horizontal motor M ₄₂ are provided.

  The substrate 10 is a table that incorporates power lines and control lines.

The mounting table 20 is disposed on the substrate 10. The mounting table 20 has a mounting surface 20S on which the culture vessel C is mounted. Here, the culture container C according to the present embodiment is a petri dish having an opening _COPEN . The culture vessel C is placed on the placement surface 20S in a state where a lid for closing the opening _COPEN is opened. The lid of the culture vessel C is opened in advance by a user's manual operation or by a mechanism for opening the lid.

  In the following, a direction substantially orthogonal to the placement surface 20S is referred to as “vertical direction”, and a direction substantially horizontal to the placement surface 20S is referred to as “horizontal direction”.

The first culture tool 30 is a member formed in a cylindrical shape and functions as a pipette. The tip 30 _TIP of the first culture tool 30 is open. The first cultivation tool 30 is accessed culture vessel C through the opening _{C OPEN.}

The first vertical drive unit 31 and the first horizontal drive unit 32 are disposed on the substrate 10. The first vertical drive unit 31 by a driving force transmitted from the first vertical motor M _31, moving the first culture tool 30 in the vertical direction. The first horizontal driving unit 32, by the driving force transmitted from the first horizontal motor M _32, moving the first culture tool 30 in the horizontal direction. Accordingly, the first culture tool 30 is three-dimensionally movable by the first vertical drive unit 31 and the first horizontal drive unit 32.

  The support part 33 supports the first culture tool 30. Specifically, the upper end of the support part 33 is connected to the first culture tool 30, and the lower end part of the support part 33 is fixed to the first vertical drive part 31.

  The liquid feeding device 34 controls the liquid feeding in the first culture tool 30. Specifically, the liquid feeding device 34 includes a pump P that supplies liquid to the first culture tool 30 and sucks the liquid from the first culture tool 30. The pump P only needs to be able to pressurize and depressurize the inside of the first culture tool 30, and may be a peristaltic pump or a diaphragm pump, for example. It should be noted that the liquid delivery device 34 has a plurality of reagent tanks (not shown), and each of the plurality of reagent tanks stores a reagent (such as a medium) used for cell culture. The liquid feeding device 34 can appropriately supply the reagent stored in each of the plurality of reagent tanks to the first culture tool 30.

The second culture tool 40 is used for a peeling operation for peeling the cell group from the culture surface of the culture vessel C. Specifically, the tip 40 _TIP of the second culture tool 40 is formed in a thin plate shape and functions as a scraper. By rubbing the tip 40 _TIP on the culture surface of the culture vessel C, the cell group attached to the culture surface can be peeled off. The second culture tool 40 is accessed in the culture vessel C through the opening _COPEN .

The second vertical drive unit 41 and the second horizontal drive unit 42 are disposed on the substrate 10. The second vertical drive unit 41, by the driving force transmitted from the second upper and lower motor M _41, moves the second culture tool 40 in the vertical direction. The second horizontal driving unit 42, by the driving force transmitted from the second horizontal motor M _42, moves the second culture tool 40 in the horizontal direction. Therefore, the second culture tool 40 is three-dimensionally movable by the second vertical drive unit 41 and the second horizontal drive unit 42.

In addition, the first vertical drive unit 31 includes a movable unit 31 _MOVE that moves together with the first culture tool 30. The movable portion 31 _MOVE is connected to the lower end portion of the support portion 33. The first cultivation tool 30, the vertical movement of the movable portion _{31 MOVE,} which is movable in the vertical direction. Further, the first horizontal drive unit 32 has a movable unit 32 _MOVE that moves together with the first culture tool 30. The movable part 32 _MOVE is connected to the lower end part of the first vertical drive part 31. The first culture tool 30 is movable in the horizontal direction by the horizontal movement of the movable part 32 _MOVE .

Similarly, the second vertical drive unit 41 has a movable unit 41 _MOVE that moves together with the second culture tool 40. The movable part 41 _MOVE is connected to the support part 40 _SUPP of the second culture tool 40. The second culture tool 40 is movable in the vertical direction by the vertical movement of the movable part 41 _MOVE . Further, the second horizontal drive unit 42 has a movable unit 42 _MOVE that moves together with the second culture tool 40. The movable part 42 _MOVE is connected to the lower end part of the second vertical drive part 41. The second culture tool 40, the horizontal movement of the movable portion _{42 MOVE,} which is movable in the horizontal direction.

Here, in the present embodiment, each of the movable portion 31 _MOVE , the movable portion 32 _MOVE , the movable portion 41 _MOVE, and the movable portion 42 _MOVE is disposed below the reference position SP set above the placement surface 20S. Yes. The reference position SP can be set based on the position in the vertical direction of the opening _COPEN provided in the culture vessel C.

In the present embodiment, the reference position SP is set to the position of the opening _COPEN of the culture vessel C placed on the placement surface 20S, but is not limited to this. For example, by setting the opening C _OPEN culture container C to the reference position SP, since no abrasion powder from above the opening C _OPEN is falling, abrasion powder to the culture vessel C is never mixed. Accordingly, the reference position SP is set from the position of the opening C _OPEN of the culture vessel C to a position that takes into consideration that no abrasion powder enters. When the height of the opening _COPEN of the culture vessel C is 4 cm, the reference position SP is preferably set to about 3 cm, which is lower than 4 cm. Further, when the distance from the culture vessel C to the movable part is long, the reference position SP may be set several cm higher than the opening _COPEN of the culture vessel C.

  Although not shown, the automatic culture apparatus 100 includes a control device that controls the movements of the first vertical drive unit 31, the first horizontal drive unit 32, the second vertical drive unit 41, and the second horizontal drive unit 42. ing. Such a control device may be built in the substrate 10.

(Operation of automatic culture equipment)
Next, operation | movement of the automatic culture apparatus 100 which concerns on 1st Embodiment is demonstrated through description of the cell culture method. Therefore, in the following, it should be noted that the operation by the user is included in addition to the operation of the automatic culture apparatus 100.

  FIG. 2 is a flowchart for explaining the schematic operation of the cell culture method according to the first embodiment.

  As shown in FIG. 2, in step S <b> 10, the cells are seeded in the culture vessel C by suspending the cells in the medium.

In step S20, cells are cultured in the culture container C by storing the culture container C in an incubator (37 ° C., CO ₂ 5% atmosphere) for a predetermined number of days.

  In step S30, cells are collected from the culture vessel C, and a predetermined number of cells suspended in the medium are seeded in the new culture vessel C. In the present embodiment, the automatic culture apparatus 100 is used in this step (hereinafter referred to as “passaging step”).

In step S40, cells are cultured in the new culture container C by storing the new culture container C in an incubator (37 ° C., CO ₂ 5% atmosphere) for a predetermined number of days.

  The cells are cultured by repeating the above steps S10 to S40.

  Below, the operation | movement of the automatic culture apparatus 100 is demonstrated through the subculture process of step S30. FIG. 3 is a flowchart for explaining the details of the passage step. In this embodiment, it should be noted that the operations of the automatic culture apparatus 100 are steps S31 to S33 and S38, and steps S34 to S37 are operations by the user.

In step S31, the automatic culture apparatus 100 moves the tip 30 _TIP of the first culture tool 30 into the culture medium in the culture container C and sucks the culture medium to discharge the culture medium from the culture container C. When the opening _COPEN of the culture vessel C is closed with a lid, the lid is opened in advance by a user's manual operation or by a mechanism for opening the lid.

In step S <b> 32, the automatic culture apparatus 100 moves the tip 40 _TIP of the second culture tool 40 along the culture surface of the culture container C to peel off the cell group attached to the culture surface of the culture container C.

  In step S <b> 33, the automatic culture apparatus 100 supplies and suspends the medium from the first culture tool 30 into the culture container C, and then collects the suspension in the centrifuge tube using the first culture tool 30.

  In step S34, the cells and the medium are separated by applying the centrifuge tube to a centrifuge.

  In step S35, the supernatant (medium) is discharged from the centrifuge tube.

  In step S36, the cells in the centrifuge tube are suspended in the medium, and an appropriate amount of the suspension is sucked to count the number of cells.

  In step S37, a medium is added so that the cell density becomes a predetermined value.

In step S38, the suspension with the adjusted cell density is supplied to a new culture vessel C. Thereafter, the opening _COPEN of the culture vessel C is closed with a lid, and the operation is completed.

(Function and effect)
In the automatic culture apparatus 100 according to the first embodiment, each of the movable part 31 _MOVE , the movable part 32 _MOVE , the movable part 41 _MOVE and the movable part 42 _MOVE is below the reference position SP set above the placement surface 20S. Is arranged.

Therefore, by setting the reference position SP based on the position in the vertical direction of the opening _COPEN provided in the culture container C, foreign matters such as wear powder generated by the friction between the components in each movable part are removed from the culture container. Mixing into C can be suppressed.

In this case, the reference position SP is the same as the opening C _OPEN, or when it is lower than the opening C _OPEN can be particularly effectively suppressed foreign material.

[Second Embodiment]
Next, a second embodiment of the present invention will be described with reference to the drawings. Specifically, in the second embodiment, a usage example of the automatic culture apparatus 100 according to the first embodiment will be described. The cell processing isolator and the safety cabinet are examples of the “workbench” according to the present invention.

(Cell processing isolator)
First, a cell processing isolator provided with the automatic culture apparatus 100 will be described. FIG. 4 is an external view showing a configuration of the cell processing isolator 200 according to the present embodiment.

  As shown in FIG. 4, the cell processing isolator 200 includes an automatic culture device 100, a work chamber P <b> 1, a door 201, an operation glove 202, and a centrifuge device 203.

  The work chamber P1 is completely isolated from the outside by being surrounded by a wall, and sterility is maintained. The automatic culture apparatus 100 is disposed outside the work chamber P1. On the wall of the work chamber P1, a door 201 provided between the work chamber P1 and the automatic culture apparatus 100 is formed. The door 201 is a sealed door capable of sealing the work chamber P1.

  The operation glove 202 is a step of bringing the culture vessel C into the automatic culture apparatus 100 from the work chamber P1, a step of opening the lid of the culture vessel C, or a step of operating the centrifuge tube by the user (steps S34 to S37 in FIG. 3). Used in

  The centrifugal separator 203 is disposed outside the work chamber P1. The centrifugal separator 203 is used in the passage step (step S34 in FIG. 3).

(Safety cabinet)
Next, a safety cabinet provided with the automatic culture apparatus 100 will be described. FIG. 5 is an external view showing a configuration of the safety cabinet 300 according to the present embodiment.

  As shown in FIG. 5, the safety cabinet 300 includes an automatic culture apparatus 100, a work chamber P <b> 2, a door 301, an operation window 302, a filter-equipped fan 303, and a centrifugal separator 304.

  The work chamber P2 is simply separated from the outside by being surrounded by a wall. The automatic culture apparatus 100 is disposed outside the work chamber P2. On the wall of the working chamber P2, a door 301 provided between the working chamber P2 and the automatic culture apparatus 100 is formed.

  The operation window 302 puts both hands of the user into the work chamber P2 in the process of bringing the culture vessel C into the automatic culture apparatus 100 from the work room P1 and the process of operating the centrifuge tube (steps S34 to S37 in FIG. 3). Used for.

  The filter-equipped fan 303 circulates air in the working chamber P2 while removing impurities.

  The centrifugal separator 304 is disposed outside the work chamber P2. The centrifugal separator 304 is used in the passage step (step S34 in FIG. 3).

(incubator)
Next, an incubator provided with the automatic culture apparatus 100 will be described. FIG. 6 is an external view showing the configuration of the incubator 400 according to the present embodiment. As shown in FIG. 6, the incubator 400 includes a culture chamber P3, a door U, a fan 401, a temperature adjustment device 402, a temperature / humidity sensor 403, a humidifying dish 404, a plurality of storage units 405, an automatic culture device 100, and an automatic conveyance device 150. Is provided.

  The culture chamber P3 accommodates the automatic culture device 100 and the automatic transfer device 150. The culture chamber P3 can be isolated from the outside of the incubator 400 by the door U. In the culture chamber P3, air is circulated by a fan 401. The temperature adjustment device 402 adjusts the temperature in the culture chamber P3 to a predetermined set temperature. The temperature / humidity sensor 403 detects the temperature / humidity in the culture chamber P3. The humidifying dish 404 adjusts the humidity in the culture chamber P3 to a predetermined set humidity. The plurality of storage units 405 are shelves for storing the culture container C.

The automatic transfer device 150 exchanges the culture container C between the automatic culture device 100 and the plurality of storage units 405. As shown in FIG. 6, the automatic conveyance device 150 includes a pair of gripping tools 151 and 151, a driving unit 152, a movable unit 152 _{MOVE <b> 1,} and a pair of movable units 152 _{MOVE <b> 2} .

The pair of gripping tools 151 and 151 are members for gripping the side surface of the culture vessel C. The lower ends of the pair of gripping tools 151 and 151 are supported by the pair of movable parts 152 _MOVE2 .

Drive unit 152 by the driving force of a motor built to move the movable portion _{152 MOVE 1} in the vertical and rotational directions. Accordingly, the pair of gripping tools 151 and 151 can be moved in the vertical direction and the rotation direction.

The driving unit 152 by the driving force of a motor built, the pair of the movable portion _{152 MOVE 2} each independently is slid over the moving part _{152 MOVE 1.} Thereby, the interval between the pair of gripping tools 151 and 151 can be adjusted.

Here, it should be noted that the movable portion 152 _MOVE1 and the pair of movable portions 152 _MOVE2 are disposed below the reference position SP of the culture vessel C placed on the automatic culture apparatus 100.

(Function and effect)
According to the cell processing isolator 200, the safety cabinet 300, and the incubator 400 according to the second embodiment, ensuring sterility in the work chambers P1, P2 and the culture chamber P3, effectively utilizing the work space, and turning over the culture container C, etc. It is possible to suppress human error.

  In addition, according to the cell processing isolator 200 and the safety cabinet 300 according to the second embodiment, the culture vessel C processed in the work chambers P1 and P2 can be easily carried into the automatic culture apparatus 100 and the centrifuges 203 and 304. can do.

  Moreover, according to the incubator 400 which concerns on 2nd Embodiment, the process to the culture container C by the automatic culture apparatus 100 and the cell culture by the culture container C in the culture chamber P3 can be performed automatically.

Further, according to the incubator 400 according to the second embodiment, since the movable portion 152 _MOVE1 and the pair of movable portions 152 _{MOVE2 of} the automatic conveyance device 150 are disposed below the reference position SP, the movable portion 152 _MOVE1 and the pair of movable portions 152 _MOVE1 It can suppress that a foreign material mixes into the culture container C from the movable part 152 _MOVE2 .

(Other embodiments)
Although the present invention has been described according to the above-described embodiments, it should not be understood that the descriptions and drawings constituting a part of this disclosure limit the present invention. From this disclosure, various alternative embodiments, examples and operational techniques will be apparent to those skilled in the art.

For example, in the above embodiment, the cell group is peeled off by the second culture tool 40 in step S32 of the passage step, but the present invention is not limited to this. For example, instead of step S32, a step of supplying trypsin to the culture vessel C with the first culture tool 30 and a step of peeling the cell group from the culture surface by applying vibration to the culture vessel C after a predetermined time has elapsed. Good. In this case, the automatic culture apparatus 100 may not include the second culture tool 40. Moreover, it may replace with step S32 and the process which isolate | separates the cell group adhering to the culture surface with the 2nd culture tool 40 in the predetermined | prescribed magnitude | size on the culture surface may be provided. In this case, the tip 40 _TIP of the second culture tool 40 preferably functions as a roller cutter in which a plurality of annular blades are provided on the outer peripheral surface of a cylindrical roller.

  In the above embodiment, the first culture tool 30 is three-dimensionally movable. However, the first culture tool 30 only needs to move up and down.

  Moreover, in the said embodiment, although it was decided that step S34-S37 of a subculture process was operation by a user, the automatic culture apparatus 100 performs step S34-S37 which is a process of operating a centrifuge automatically. You may have a structure.

  In the above embodiment, the first culture tool 30 that functions as a pipette and the second culture tool 40 that functions as a scraper have been described. However, the automatic culture apparatus 100 includes a culture tool that performs other functions. Also good.

Here, FIG. 7 is a diagram illustrating a configuration of the automatic culture apparatus 100 including the culture tool 50 that functions as tweezers. FIG. 7A is a side view of the automatic culture apparatus 100. FIG. 7B is a front view of the automatic culture apparatus 100. As shown in FIG. 7, the culture tool 50 includes two gripping parts 50 _TIP , a vertical driving part 51, a vertical motor M ₅₁ , a horizontal driving part 52, and a horizontal motor M ₅₂ . The vertical drive unit 51 moves the two gripping units 50 _TIP in the vertical direction by the driving force of the vertical motor M ₅₁ . The horizontal driving unit 52, by the driving force of the upper and lower motor _{M 52,} moving the two gripping portions _{50 TIP} horizontally. Accordingly, the culture tool 50 functions as tweezers for gripping various tools and transporting them into the culture vessel C by moving each of the two gripping portions 50 _TIP in the vertical direction and the horizontal direction.

  Thus, it should be understood that the present invention includes various embodiments and the like not described herein. Therefore, the present invention is limited only by the invention specifying matters in the scope of claims reasonable from this disclosure.

C: culture vessel, 10: substrate, 20: mounting table, 20S: mounting surface, 30 ... first culture tool, 30 _TIP ... tip, 31 ... first vertical drive unit, 31 _MOVE ... movable unit, 32 ... first Horizontal drive part, 32 _MOVE ... movable part, 33 ... support part, 34 ... liquid feeding device, 40 ... second culture tool, 40 _SUPP ... support part, 40 _TIP ... tip, 41 ... second vertical drive part, 41 _MOVE ... Movable part, 42 ... second horizontal drive part, 42 _MOVE ... movable part, 50 ... culture tool, 50 _TIP ... gripping part, 51 ... vertical drive part, 51 _MOVE ... movable part, 52 ... horizontal drive part, 52 _MOVE ... movable , 100 ... Automatic culture device, 150 ... Automatic transfer device, 151 ... Gripping tool, 152 _MOVE ... Moving part, 152 ... Driver, 200 ... Cell processing isolator, 201 ... Door, 202 ... Operation Globe, 203 ... centrifuge, 300 ... safety cabinet, 301 ... door, 302 ... operation window, 303 ... fan with filter, 304 ... centrifuge, 400 ... incubator, 401 ... fan, 402 ... temperature controller, 403 ... Temperature / humidity sensor, 404 ... humidifying dish, 405 ... storage section, M ... motor, P ... pump, P1, P2 ... work chamber, P3 ... culture chamber, U ... door, d ... interval

Claims (6)

An automatic culture apparatus that automatically performs a culture operation in a culture vessel in which cell culture is performed,
A mounting table having a mounting surface for mounting the culture vessel;
A culture tool placed on the table and used for cell culture;
A drive unit for moving the culture tool,
The drive unit has a movable unit that moves with the culture tool,
The movable part is disposed below a reference position set above the placement surface,
The automatic culture apparatus, wherein the reference position is set based on a position in an up-down direction of an opening provided in the culture vessel. The culture tool is a cylindrical member,
A pump for supplying a fluid to the culture tool and aspirating the fluid from the culture tool;
A support unit fixed to the drive unit and supporting the culture tool;
The automatic culture apparatus according to claim 1, wherein the driving unit moves the support unit in a vertical direction. The tip of the culture tool is a scraper or a roller cutter,
The automatic culture apparatus according to claim 1, wherein the driving unit moves the tip in the vertical direction and the horizontal direction. The culture tool has a plurality of gripping portions,
The automatic culture apparatus according to claim 1, wherein the driving unit moves each of the plurality of gripping units in a vertical direction and a horizontal direction. A work room surrounded by walls,
An automatic culture apparatus that is arranged outside the working chamber and automatically performs a culture operation in a culture vessel in which cell culture is performed;
A door provided on the wall between the working chamber and the automatic culture apparatus;
The automatic culture apparatus includes:
A mounting table having a mounting surface for mounting the culture vessel;
A culture tool disposed above the table and used for cell culture;
A drive unit for moving the culture tool,
The drive unit has a movable unit that moves with the culture tool,
The movable part is disposed below a reference position set above the placement surface,
The work table characterized in that the reference position is set based on a position in an up-down direction of an opening provided in the culture vessel. A culture room having a plurality of storage units;
An automatic culture apparatus that is arranged in the culture chamber and automatically performs a culture operation in a culture vessel in which cell culture is performed;
A temperature control device for adjusting the temperature in the culture chamber;
An automatic transfer device for transferring the culture vessel between the plurality of storage units and the automatic culture device;
The automatic culture apparatus includes:
A mounting table having a mounting surface for mounting the culture vessel;
A culture tool disposed above the table and used for cell culture;
A drive unit for moving the culture tool,
The drive unit has a movable unit that moves with the culture tool,
The movable part is disposed below a reference position set above the placement surface,
The incubator, wherein the reference position is set based on a position in an up-down direction of an opening provided in the culture vessel.

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