CN114471772A - Water bath shaking table for extracting immune globulin from egg yolk - Google Patents
Water bath shaking table for extracting immune globulin from egg yolk Download PDFInfo
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- CN114471772A CN114471772A CN202210177934.7A CN202210177934A CN114471772A CN 114471772 A CN114471772 A CN 114471772A CN 202210177934 A CN202210177934 A CN 202210177934A CN 114471772 A CN114471772 A CN 114471772A
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/02—Water baths; Sand baths; Air baths
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/02—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from eggs
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- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
- G05D27/00—Simultaneous control of variables covered by two or more of main groups G05D1/00 - G05D25/00
- G05D27/02—Simultaneous control of variables covered by two or more of main groups G05D1/00 - G05D25/00 characterised by the use of electric means
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/11—Immunoglobulins specific features characterized by their source of isolation or production isolated from eggs
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Abstract
The invention discloses a water bath table concentrator for extracting immune globulin from egg yolk, in particular to the field of biological cultivation, which comprises a water bath adjusting mechanism and a water bath control mechanism, wherein the water bath adjusting mechanism comprises a curved clamping plate block, the outer side of the curved clamping plate block is clamped with a curved movable push plate, the inner side of the curved movable push plate is rotatably connected with a threaded rotating rod, the two sides of the curved movable push plate are rotatably connected with braking clamping plates through the threaded rotating rod of the curved movable push plate, the inner side of the braking clamping plates is rotatably connected with a limiting clamping plate through the threaded rotating rod of the curved movable push plate, a water injection frame puts water into a supporting outer frame part, as the water temperature is gradually heated to the lower surface by the upper surface, the curved movable push plate drives the braking clamping plates to deflect through the limiting clamping plates, the braking clamping plates drive an auxiliary cultivation frame on the upper surface to float upwards through the positioning clamping plates, the auxiliary cultivation shelf is preferentially contacted with the re-poured water to restore the temperature thereof to normal.
Description
Technical Field
The invention relates to the technical field of biological cultivation, in particular to a water bath shaking table for extracting immune globulin from egg yolk.
Background
The water bath shaking table is a biochemical instrument combining a constant temperature water bath with controllable temperature and an oscillator, and is mainly suitable for culturing various microorganisms.
However, in the existing water bath living environment for extracting immunoglobulin from egg yolk, the requirement on temperature is high, the temperature is 55-65 ℃, most of water bath shaking tables are used in cultivation mechanisms such as schools and the like, the internal conditions of equipment cannot be paid attention to at all times, and in the process of using the water bath shaking tables, the clamping force of a clamping mechanism of a cultivation bin is kept consistent in the shaking and vibrating process, and the cultivation bin is easy to separate and fall and damage when the shaking amplitude is large under the condition of keeping shaking for a long time, so that the water bath shaking table for extracting immunoglobulin from egg yolk is provided.
Disclosure of Invention
In order to overcome the above-mentioned defects in the prior art, an embodiment of the present invention provides a water bath shaking table for extracting immunoglobulin from egg yolk, wherein water is poured into a supporting outer frame member through a water injection frame, because the water temperature is such that the upper surface gradually warms to the lower surface, a curved movable push plate drives a braking clamping plate to deflect through a limiting clamping plate, the braking clamping plate drives an auxiliary cultivation frame on the upper surface to float upwards through a positioning clamping plate, the auxiliary cultivation frame preferentially contacts with the re-poured water to recover the temperature thereof to normal, and the sliding deflection frame drives several layers of auxiliary cultivation frames to float upwards through arrangement of clamping grooves to solve the problems in the background art.
In order to achieve the purpose, the invention provides the following technical scheme: a water bath shaking table for extracting immune globulin from egg yolk comprises a water bath adjusting mechanism and a water bath control mechanism, wherein the water bath adjusting mechanism comprises a curved clamping plate block, a curved movable push plate is clamped on the outer side of the curved clamping plate block, a threaded rotating rod is rotatably connected to the inner side of the curved movable push plate, two sides of the curved movable push plate are rotatably connected with a braking clamping plate through the threaded rotating rod of the curved movable push plate, the inner side of the braking clamping plate is rotatably connected with a limiting clamping plate through the threaded rotating rod of the curved movable push plate, the braking clamping plate and the limiting clamping plate are arranged in a synchronous deflection state, a positioning clamping plate is arranged on one side of the braking clamping plate, a sliding deflection frame is connected to the outer side of the braking clamping plate through bolts, an installation clamping groove is formed in the outer side of the sliding deflection frame, and a positioning connecting plate is clamped on one side of the curved movable push plate;
the water bath control mechanism comprises a water flow storage frame, a movable water pipe is inserted into the inner side of the water flow storage frame, a movable rotary valve is inserted into one side of the movable water pipe, water injection frames are inserted into the two sides of the movable rotary valve, a curved rotary handle is connected to the outer side of the movable rotary valve in a rotating mode, the curved rotary handle is clamped to one side of the positioning connection plate, and the water injection frame is arranged in a water bath heating state through the curved rotary handle and the deflection force of the movable rotary valve.
The beneficial effect of adopting the further scheme is that: bent shape cardboard piece drives the location connecting plate through bent shape activity push pedal and deflects, the location connecting plate drives the activity through bent shape runner handle and changes the valve rotation, the activity is changeed the valve rotation and is caused the inside not receiving of water injection frame and is blockked, then the inside water of rivers storage frame puts in normal temperature's water bath through movable water pipe, the water injection frame drops into water and supports in the frame spare, because the temperature is that the upper surface gradually sends the lower surface with the temperature, then bent shape activity push pedal drives the braking cardboard through spacing cardboard and realizes deflecting, the braking cardboard drives the supplementary culture rack of upper surface through the location cardboard upwards floating, then supplementary culture rack priority contact water of pouring into again, make its temperature resume normal.
In a preferred embodiment, the bottom end of the water bath control mechanism is connected with a water bath table support mechanism in a clamping manner, the inner side of the water bath table support mechanism is provided with an auxiliary shaking mechanism, the inner side of the auxiliary shaking mechanism is connected with an immune ball cultivation mechanism in a rotating manner, the water bath control mechanism is connected to one side of a water bath adjusting mechanism in a clamping manner, and the inner side of the water bath adjusting mechanism is connected with a heat preservation mechanism in a rotating manner.
The beneficial effect of adopting the further scheme is that: the linkage of the mechanism is improved.
In a preferred embodiment, the water bath shaking table supporting mechanism comprises a supporting outer frame member, wherein a supporting clamping frame is welded in the center of the inner side of the supporting outer frame member, limiting baffle frames are clamped on two sides of the supporting clamping frame, and the limiting baffle frames are clamped on the outer side of a water flow storage frame.
The beneficial effect of adopting the further scheme is that: the inner side of the supporting outer frame piece is communicated with warm water with the penetration degree of minus degree, and meanwhile, the electric energy drives the outer supporting frame to deflect and oscillate.
In a preferred embodiment, the auxiliary shaking mechanism comprises an external support frame, a movable slide way frame is arranged on the outer side of the external support frame, a bidirectional movable clamping column is clamped on the outer side of the external support frame through the movable slide way frame, a slide way is arranged on the inner side of the bidirectional movable clamping column, two sliding clamping frames are clamped on the outer side of the bidirectional movable clamping column through the slide way of the bidirectional movable clamping column, and telescopic clamping rods are inserted into two sides of the two sliding clamping frames.
The beneficial effect of adopting the further scheme is that: the external support frame guides the bidirectional movable clamping column to realize deflection and oscillation through the movable slideway frame, and the bidirectional movable clamping column drives the multi-layer auxiliary cultivation frame to realize synchronous deflection and oscillation through the sliding clamping frame and the telescopic clamping rod.
In a preferred embodiment, immunity ball cultivation mechanism includes supplementary culture rack, the quantity of supplementary culture rack is the multiunit, the multiunit the inboard welding of supplementary culture rack has the partition board, the multiunit the inboard of supplementary culture rack is pegged graft and is had multiunit card to hold to press from both sides to get and press from both sides, the inboard of pressing from both sides the clamp to press from both sides is pegged graft and is had the screw thread dwang, peg graft at the both ends of screw thread dwang and have supplementary piece, two the slip card frame is pegged graft in the outside of supplementary culture rack through flexible calorie pole, the multiunit the card is held to press from both sides and is pressed from both sides the pulling of pressing from both sides through the screw thread dwang and be interval adjustment state setting, the multiunit supplementary culture rack is the state setting of rocking through the synchronous deflection of slip card frame with outside support frame, supplementary culture rack joint is in the outside of location cardboard, the multiunit the surface of supplementary culture rack is pegged graft and is had the telescopic link.
The beneficial effect of adopting the further scheme is that: the threaded rotating rod is connected with the clamping plate of the telescopic clamping rod, the connecting part of the threaded rotating rod and the auxiliary accommodating block is in threaded connection, the thread of the threaded rotating rod is a forward thread, the telescopic clamping rod drives the threaded rotating rod to deflect through the auxiliary accommodating block, the connecting part of the threaded rotating rod and the clamping clamp is connected through threaded engagement, the connecting part of the clamping clamp is a reverse thread, rolling friction force is generated between the threaded rotating rod and the clamping clamp, the clamping clamp moves inwards due to the reverse direction of the thread, the clamping clamp clamps the cultivation bin to deflect, and the clamping force of the cultivation bin is stronger when the deflection amplitude is larger.
In a preferred embodiment, the heat preservation mechanism comprises a clamping outer frame, an annular air bag frame is inserted into the inner side of the clamping outer frame, a plurality of groups of heat conducting holes are formed in the outer surface of the clamping outer frame, the annular air bag frame is arranged in a state of expansion with heat and contraction with cold through the heat conducting holes of the clamping outer frame, and a movable push rod is inserted into the inner side of the annular air bag frame.
The beneficial effect of adopting the further scheme is that: the movable push rod drives the electromagnetic movable frame to move outwards through the torque spring frame, the electromagnetic movable frame is pressed down to the position of the movable push rod, the electromagnetic movable frame is attracted to surface metal of the position of the movable push rod through magnetic attraction, and meanwhile, the internal heat of the circular air bag frame is rapidly conducted to the position of the electromagnetic movable frame, so that the electromagnetic movable frame gives an alarm according to the change of air temperature.
In a preferred embodiment, the heat preservation mechanism further comprises an auxiliary central gear, the outer side of the auxiliary central gear is rotatably connected with a friction rotating wheel, the outer surface of the friction rotating wheel is provided with an air bag pushing frame, the inner side of the air bag pushing frame is inserted with a rubber receiving pushing block, the outer side of the air bag pushing frame is inserted with a brake piston, the inner side of the brake piston is inserted with a positioning movable push rod, the two sides of the positioning movable push rod are provided with a torque spring frame, the outer side of the torque spring frame is spliced with an electromagnetic movable frame, the positioning movable push rod is arranged in a magnetic attraction state by reducing the distance between the positioning movable push rod and the electromagnetic movable frame, the electromagnetic movable frame is clamped at the outer side of the movable push rod, the outer side of the friction rotating wheel is provided with a connecting rod, the friction rotating wheel is clamped on the outer side of the curved clamping plate block through the connecting rod of the friction rotating wheel, and the friction rotating wheel and the curved clamping plate block are arranged in a synchronous deflection state.
The beneficial effect of adopting the further scheme is that: the positioning movable push rod drives the rubber folding mechanism in the air bag pushing frame to change from a contraction state to an outward moving state by pressing air pressure in the braking piston, the air bag pushing frame and the friction rotating wheel in an expansion state generate rolling friction force through the auxiliary central gear to rotate clockwise, and if the temperature drops, the rubber folding mechanism of the air bag pushing frame contracts inwards by the air pressure, and the auxiliary central gear rotates anticlockwise in the same way.
In a preferred embodiment, a water bath shaker debugging method for extracting immunoglobulin from egg yolk,
s1, respectively placing a temperature control module A and a vibration frequency monitoring module A at the liquid storage position of the water bath shaking table, and arranging a temperature control module B and a vibration frequency monitoring module B at the nearest position of the material placement;
s2, the temperature control module A, the vibration frequency monitoring module A, the temperature control module B and the vibration frequency monitoring module B carry out data debugging through a controller and a signal receiving module in the equipment;
s3, setting a proper temperature range of the material to be 45-65 ℃, and generating a text set of the proper temperature range;
s4, sequentially monitoring a temperature control module A and a temperature control module B, a vibration frequency monitoring module A and a vibration frequency monitoring module B in a temperature interval on a text set of a proper temperature interval, and generating a temperature difference text set and a vibration frequency difference text set;
calculating the absolute value of the difference between the temperature control module A and the temperature control module B according to different temperature nodes set by the heating equipment in the temperature difference text set;
calculating the absolute value of the difference between the vibration frequency monitoring module A and the vibration frequency monitoring module B by using different rotating speeds or vibration frequency nodes set by a motor of equipment generating vibration in the vibration frequency difference text set;
s5, selecting 3-5 temperature or rotating speed nodes or shaking frequency nodes with the minimum difference according to the temperature difference text set and the shaking frequency difference text set to generate an optimal temperature node text set and an optimal shaking frequency text set;
s6 selects and sets different values in the text set of the optimum temperature node and the text set of the optimum vibration frequency.
In a preferred embodiment, values are generated for parameters of external factors including, temperature outside the apparatus, humidity inside the apparatus, humidity outside the apparatus, altitude of the area where the apparatus is used, and combined into a linear combination in S6;
linearly combining the randomly generated external factor parameters, then constructing a random forest by taking the random forest as an input variable, selecting N variables A, B, C, D.. Z and N random numbers O on each parameter node, linearly combining to obtain a variable Y,
Ni∈[-1,+1]
wherein i is a serial number, a corresponding variable is obtained according to the external factor parameter which is randomly generated, and the value of the external factor parameter T is randomly extracted,
T＝log2R
r is the total number of the external factor parameters of the input variables, a state that the variable Y is cracked downwards is formed by the value of T, finally, the corresponding variable Y is generated according to the parameters of different external factors, and the value of the text set corresponding to the optimal temperature node and the text set corresponding to the optimal vibration frequency is selected through the variable Y according to the preset value.
The invention has the technical effects and advantages that:
1. the water injection frame puts water into the supporting outer frame part, because the water temperature is the lower surface caused by the gradual same temperature of the upper surface, the curved movable push plate drives the brake clamping plate to deflect through the limiting clamping plate, the brake clamping plate drives the auxiliary cultivation frame on the upper surface to float upwards through the positioning clamping plate, the auxiliary cultivation frame preferentially contacts the re-injected water to restore the temperature to normal, and the sliding deflection frame drives the auxiliary cultivation frames on several layers to float upwards through the arrangement clamping grooves, so that the first aid of the cultivation bin in the equipment is realized, the cultivation failure is avoided, and the cultivation efficiency is improved;
2. the electromagnetic movable frame is attracted to the surface metal of the positioning movable push rod through the magnetic attraction, meanwhile, the internal heat of the circular air bag frame is quickly conducted to the electromagnetic movable frame, so that the electromagnetic movable frame gives an alarm according to the change of the air temperature to remind researchers, and meanwhile, when the water bath is cooled due to external influence, the circular air bag frame contracts in the same way, so that the electromagnetic movable frame senses the change of the air temperature, and the internal detection of equipment can be better realized;
3. the rolling friction force is that the clamping clamp moves towards the inner side due to the reverse direction of the threads, the clamping clamp clamps the clamping clamp to tightly grasp the cultivation bin to deflect, the larger the deflection amplitude is, the stronger the clamping force on the cultivation bin is, and therefore the situation that the cultivation bin is easy to separate and further to drop and damage under the condition that the oscillation amplitude is large and the oscillation condition is kept for a long time is avoided.
4. And determining values of the final optimal temperature node text set and the optimal vibration frequency text set according to the parameter generation value of the external factor, determining the value ranges of the optimal temperature node text set and the optimal vibration frequency text set in an error mode, and selecting the most appropriate temperature node and vibration frequency node according to the parameter of the external factor, so that the convenience of temperature and vibration frequency selection and determination is improved.
Drawings
FIG. 1 is a schematic structural diagram of the supporting mechanism of the water bath rocking bed of the present invention.
FIG. 2 is an enlarged view of the portion A of FIG. 1 according to the present invention.
Fig. 3 is a schematic structural view of the sliding deflection frame of the present invention.
FIG. 4 is a schematic diagram of an auxiliary shaking mechanism according to the present invention.
FIG. 5 is an enlarged view of the portion B of FIG. 2 according to the present invention.
Figure 6 is a schematic view of the curved wheel handle of the present invention.
Fig. 7 is a schematic view of the auxiliary center gear of the present invention.
FIG. 8 is an enlarged view of the structure of the portion C of FIG. 7 according to the present invention.
The reference signs are: 1. a water bath shaking table supporting mechanism; 101. supporting the outer frame member; 102. a support clip frame; 103. a limiting baffle frame; 2. an auxiliary shaking mechanism; 201. an outer support frame; 202. a bidirectional movable clamping column; 203. a sliding clamp frame; 204. a telescopic clamping rod; 205. a movable slideway rack; 3. a water bath adjusting mechanism; 301. a curved cardboard block; 302. positioning a clamping plate; 303. a sliding deflection frame; 304. arranging a clamping groove; 305. a curved movable push plate; 306. a limiting clamping plate; 307. a brake catch plate; 308. positioning the connecting plate; 4. a water bath control mechanism; 401. a water flow storage rack; 402. a movable water pipe; 403. a movable rotary valve; 404. a curved runner handle; 405. a water injection frame; 5. an immune ball cultivation mechanism; 501. an auxiliary cultivation shelf; 502. clamping the clamping clip; 503. dividing the plate; 504. an auxiliary storage block; 505. a threaded rotating rod; 6. a heat preservation mechanism; 601. clamping the outer frame; 602. a circular air bag holder; 603. a movable push rod; 604. a brake piston; 605. a friction rotating wheel; 606. a torque spring carrier; 607. positioning the movable push rod; 608. an auxiliary central gear; 609. an air bag pushing frame; 610. an electromagnetic movable frame.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 5 to 6, embodiment 1: a water bath shaking table for extracting immune globulin from egg yolk comprises a water bath adjusting mechanism 3 and a water bath control mechanism 4, wherein the water bath adjusting mechanism 3 comprises a curved clamping plate block 301, a curved movable push plate 305 is clamped on the outer side of the curved clamping plate block 301, the inner side of the curved movable push plate 305 is rotatably connected with a threaded rotating rod, two sides of the curved movable push plate 305 are rotatably connected with a braking clamping plate 307 through the threaded rotating rod of the curved movable push plate 305, the inner side of the braking clamping plate 307 is rotatably connected with a limiting clamping plate 306 through the threaded rotating rod of the curved movable push plate 305, the braking clamping plate 307 and the limiting clamping plate 306 are arranged in a synchronous deflection state, one side of the braking clamping plate 307 is provided with a positioning clamping plate 302, the outer side of the braking clamping plate 307 is connected with a sliding deflection frame 303 through a bolt, the outer side of the sliding deflection frame 303 is provided with a mounting clamping groove 304, and one side of the curved movable push plate 305 is clamped with a positioning connecting plate 308;
the water bath control mechanism 4 comprises a water flow storage frame 401, a movable water pipe 402 is inserted into the inner side of the water flow storage frame 401, a movable rotary valve 403 is inserted into one side of the movable water pipe 402, water injection frames 405 are inserted into the two sides of the movable rotary valve 403, a curved rotary handle 404 is rotatably connected to the outer side of the movable rotary valve 403, the curved rotary handle 404 is clamped on one side of the positioning connection plate 308, and the water injection frames 405 are arranged in a water bath heating state through the curved rotary handle 404 and the deflection force of the movable rotary valve 403.
It should be noted that, the friction wheel 605 drives the curved cardboard block 301 to deflect through the external connecting rod, the curved cardboard block 301 drives the positioning connecting plate 308 to deflect through the curved movable pushing plate 305, the positioning connecting plate 308 drives the movable rotating valve 403 to rotate through the curved wheel handle 404, the movable rotating valve 403 rotates to cause the interior of the water injection rack 405 not to be blocked, then the water in the water storage rack 401 is thrown into the water bath with normal temperature through the movable water pipe 402, the water injection rack 405 throws the water into the supporting outer frame member 101, because the water temperature is that the upper surface gradually warms to the lower surface, the curved movable pushing plate 305 drives the braking cardboard 307 to deflect through the limiting cardboard 306, the braking cardboard 307 drives the auxiliary cultivation rack 501 on the upper surface to float upwards through the positioning cardboard 302, the auxiliary cultivation rack 501 preferentially contacts the refilled water to recover the normal temperature, the sliding deflection rack 303 drives several layers of auxiliary cultivation racks 501 to float upwards through the arrangement clamping groove 304, thereby realized carrying out the first aid to the inside cultivation storehouse of equipment fast, avoided cultivating the failure, improved and cultivated efficiency.
Referring to fig. 7 to 8, embodiment 2: the bottom end of the water bath control mechanism 4 is clamped with a water bath shaking table supporting mechanism 1, the inner side of the water bath shaking table supporting mechanism 1 is provided with an auxiliary shaking mechanism 2, the inner side of the auxiliary shaking mechanism 2 is rotatably connected with an immune ball cultivating mechanism 5, the water bath control mechanism 4 is clamped at one side of a water bath adjusting mechanism 3, the inner side of the water bath adjusting mechanism 3 is rotatably connected with a heat preservation mechanism 6, the heat preservation mechanism 6 comprises a clamping outer frame 601, the inner side of the clamping outer frame 601 is inserted with an annular air bag frame 602, the outer surface of the clamping outer frame 601 is provided with a plurality of groups of heat transmission holes, the annular air bag frame 602 is arranged in a state of thermal expansion and cold contraction through the heat transmission holes of the clamping outer frame 601, the inner side of the annular air bag frame 602 is inserted with a movable push rod 603, the heat preservation mechanism 6 further comprises an auxiliary central gear 608, the outer side of the auxiliary central gear 608 is rotatably connected with a friction rotating wheel 605, the outer surface of the friction rotating wheel 605 is provided with an air bag push frame 609, the inboard of gasbag pushing frame 609 is pegged graft and is had the rubber to accomodate the ejector pad, the outside of gasbag pushing frame 609 is pegged graft and is had brake piston 604, the inboard of brake piston 604 is pegged graft and is had location activity push rod 607, the both sides of location activity push rod 607 are provided with moment of torsion spring frame 606, the outside of moment of torsion spring frame 606 is pegged graft and is had electromagnetism movable frame 610, location activity push rod 607 is through the reduction of the interval with electromagnetism movable frame 610 and be the setting of magnetic attraction state, electromagnetism movable frame 610 joint is in the outside of activity push rod 603, the outside of friction runner 605 is provided with the connecting rod, friction runner 605 passes through the connecting rod joint of friction runner 605 in the outside of curved shape cardboard piece 301, friction runner 605 is the setting of synchronous deflection state with curved shape cardboard piece 301.
It should be noted that, when the temperature of the water bath is raised due to external influence, the annular airbag frame 602 is isolated from the external water bath by the retaining outer frame 601, because the specific heat capacity of water is greater than that of air, the air is subjected to temperature change more quickly, the annular airbag frame 602 preferably senses the temperature change, a conventional temperature sensor is replaced, the temperature sensor is used for conducting heat detection through a thermistor, the annular airbag frame is usually wrapped in the equipment and cannot sense the external temperature change in the first time, when the temperature of the water bath is too high, immunoglobulin is more volatile and inactive, the air pressure in the annular airbag frame 602 senses the temperature difference, the movable push rod 603 is driven to move inwards by thermal expansion, a temperature detecting instrument is arranged in the electromagnetic movable frame 610, the model is FY10FY11FY12, the movable push rod 603 drives the electromagnetic movable frame 610 to move outwards by the torque spring frame 606, and the electromagnetic movable frame 610 presses down to the positioning push rod 607, the electromagnetic movable frame 610 is attracted to the surface metal of the positioning movable push rod 607 through magnetic attraction, meanwhile, the internal heat of the circular air bag frame 602 is rapidly conducted to the electromagnetic movable frame 610, so that the electromagnetic movable frame can give an alarm according to the change of air temperature to remind researchers, meanwhile, when the water bath is cooled due to external influence, the circular air bag frame 602 contracts in the same way, so that the electromagnetic movable frame 610 feels the change of air temperature, and the internal detection of equipment can be better realized.
The positioning movable push rod 607 pushes the air pressure in the brake piston 604 down, the air pressure in the brake piston 604 drives the rubber folding mechanism in the air bag push frame 609 to change from a contraction state to an outward moving state by the air pressure of the air flow, the air bag push frame 609 and the friction rotating wheel 605 in an expansion state generate rolling friction force through the auxiliary central gear 608 to rotate clockwise, if the temperature is reduced, the rubber folding mechanism of the air bag push frame 609 contracts inwards by the air pressure of the air flow, and the auxiliary central gear 608 rotates anticlockwise in the same way.
Referring to fig. 1-4, example 3: the water bath shaking table supporting mechanism 1 comprises a supporting outer frame part 101, a supporting clamping frame 102 is welded in the center of the inner side of the supporting outer frame part 101, limiting baffle frames 103 are clamped on two sides of the supporting clamping frame 102, the limiting baffle frames 103 are clamped on the outer side of a water flow storage frame 401, an auxiliary shaking mechanism 2 comprises an outer supporting frame 201, a movable slide way frame 205 is arranged on the outer side of the outer supporting frame 201, a bidirectional movable clamping column 202 is clamped on the outer side of the outer supporting frame 201 through the movable slide way frame 205, a slide way is arranged on the inner side of the bidirectional movable clamping column 202, two sliding clamping frames 203 are clamped on the outer side of the bidirectional movable clamping column 202 through the slide way of the bidirectional movable clamping column 202, telescopic clamping rods 204 are inserted in two sides of the two sliding clamping frames 203, an immune ball cultivating mechanism 5 comprises auxiliary cultivating frames 501, the number of the auxiliary cultivating frames 501 is multiple groups, partition plates 503 are welded on the inner sides of the multiple groups of the auxiliary cultivating frames 501, multiple groups of clamping clamps 502 are inserted and clamped, the inboard of the clamp of card dress is got and is pegged graft threaded dwang 505, the both ends of threaded dwang 505 are pegged graft and are had supplementary piece 504 of accomodating, two slip card framves 203 are pegged graft in the outside of supplementary culture rack 501 through flexible kelly 204, the pulling that the clamp 502 was got through threaded dwang 505 is interval adjustment state setting for the clamp of multiunit card dress, multiunit supplementary culture rack 501 is the state setting of rocking through the synchronous deflection of slip card frame 203 with outside support frame 201, supplementary culture rack 501 joint is in the outside of location cardboard 302, the surface of multiunit supplementary culture rack 501 is pegged graft and is had the telescopic link.
It should be noted that, inside of the supporting outer frame 101 is introduced with warm water of 55-65 degrees, and at the same time, electric energy drives the outer supporting frame 201 to deflect and oscillate, the outer supporting frame 201 guides the two-way movable clamping column 202 to deflect and oscillate through the movable slideway 205, and the two-way movable clamping column 202 drives the multi-layer auxiliary cultivation frame 501 to synchronously deflect and oscillate through the sliding clamping frame 203 and the telescopic clamping rod 204, the clamping clamp 502 clamps and mounts the cultivation chamber, the port of the telescopic clamping rod 204 is provided with a clamping plate, the threaded rotating rod 505 is connected with the clamping plate of the telescopic clamping rod 204, the connecting part of the threaded rotating rod 505 and the auxiliary receiving block 504 is in threaded connection, the thread of the threaded rotating rod 505 is a forward thread, the telescopic clamping rod 204 drives the threaded rotating rod 505 to deflect through the auxiliary receiving block 504, and the connecting part of the threaded rotating rod 505 and the clamping clamp 502 is in threaded engagement connection, the clamping device 502 is provided with a connecting part which is a reverse thread, a rolling friction force is generated between the clamping device 502 and the reverse thread, the clamping device 502 moves inwards due to the reverse thread to clamp the cultivation bin, the clamping device 502 tightly clamps the cultivation bin to deflect, the clamping force on the cultivation bin is stronger when the deflection amplitude is larger, and therefore the cultivation bin is prevented from being separated easily and further falling and damaged when the oscillation amplitude is larger under the condition of keeping oscillation for a long time.
In addition, the scheme also has a debugging method, namely a water bath shaking table debugging method for extracting the immune globulin from the egg yolk,
s1, respectively placing a temperature control module A and a vibration frequency monitoring module A at the liquid storage position of the water bath shaking table, and arranging a temperature control module B and a vibration frequency monitoring module B at the nearest position of the material placement;
s2, the temperature control module A, the vibration frequency monitoring module A, the temperature control module B and the vibration frequency monitoring module B carry out data debugging through a controller and a signal receiving module in the equipment;
s3, setting a proper temperature range of the material to be 45-65 ℃, and generating a text set of the proper temperature range;
s4, sequentially monitoring a temperature control module A and a temperature control module B, a vibration frequency monitoring module A and a vibration frequency monitoring module B in a temperature interval on a text set of a proper temperature interval, and generating a temperature difference text set and a vibration frequency difference text set;
calculating the absolute value of the difference between the temperature control module A and the temperature control module B according to different temperature nodes set by the heating equipment in the temperature difference text set;
calculating the absolute value of the difference between the vibration frequency monitoring module A and the vibration frequency monitoring module B by using different rotating speeds or vibration frequency nodes set by a motor of equipment generating vibration in the vibration frequency difference text set;
s5, selecting 3-5 temperature or rotating speed nodes or shaking frequency nodes with the minimum difference according to the temperature difference text set and the shaking frequency difference text set to generate an optimal temperature node text set and an optimal shaking frequency text set;
s6, selecting different values in the text set of the optimal temperature node and the text set of the optimal vibration frequency for setting;
meanwhile, in S6, generating values for parameters of external factors, and combining the values into a linear combination, wherein the external factors comprise the temperature outside the equipment, the humidity inside the equipment, the humidity outside the equipment and the altitude of the use area of the equipment;
linearly combining the randomly generated external factor parameters, then constructing a random forest by taking the random forest as an input variable, selecting N variables A, B, C, D.. Z and N random numbers O on each parameter node, linearly combining to obtain a variable Y,
Ni∈[-1,+1]
wherein i is a serial number, a corresponding variable is obtained according to the external factor parameter which is randomly generated, and the value of the external factor parameter T is randomly extracted,
T＝log2R
r is the total number of the external factor parameters of the input variables, a state that the variable Y is cracked downwards is formed by the value of T, finally, the corresponding variable Y is generated according to the parameters of different external factors, and the value of the corresponding optimal temperature node text set and the value of the optimal vibration frequency text set are selected through the variable Y according to the preset value;
for the above method, it is further described that values of the final optimal temperature node text set and the optimal vibration frequency text set are determined by parameter generation values of external factors, and meanwhile, value ranges of the optimal temperature node text set and the optimal vibration frequency text set are determined by means of errors, and the most appropriate temperature node and vibration frequency node are selected by using the parameters of the external factors, so that convenience in selection and determination of temperature and vibration frequency is improved.
The points to be finally explained are: first, in the description of the present application, it should be noted that, unless otherwise specified and limited, the terms "mounted," "connected," and "connected" should be understood broadly, and may be a mechanical connection or an electrical connection, or a communication between two elements, and may be a direct connection, and "upper," "lower," "left," and "right" are only used to indicate a relative positional relationship, and when the absolute position of the object to be described is changed, the relative positional relationship may be changed;
secondly, the method comprises the following steps: in the drawings of the disclosed embodiments of the invention, only the structures related to the disclosed embodiments are referred to, other structures can refer to common designs, and the same embodiment and different embodiments of the invention can be combined with each other without conflict;
and finally: the present invention is not limited to the above preferred embodiments, but rather, any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. The utility model provides a water bath shaking table that extract immunoglobulin in yolk used, includes water bath adjustment mechanism (3) and water bath control mechanism (4), its characterized in that: the water bath adjusting mechanism (3) comprises a curved clamping plate block (301), a curved movable push plate (305) is clamped and connected with the outer side of the curved clamping plate block (301), the inner side of the curved movable push plate (305) is rotationally connected with a threaded rotating rod, two sides of the curved movable push plate (305) are rotationally connected with a braking clamping plate (307) through the threaded rotating rod of the curved movable push plate (305), the inner side of the braking clamping plate (307) is rotationally connected with a limiting clamping plate (306) through a threaded rotating rod of a curved movable push plate (305), the braking clamping plate (307) and the limiting clamping plate (306) are arranged in a synchronous deflection state, one side of the braking clamping plate (307) is provided with a positioning clamping plate (302), the outer side of the braking clamping plate (307) is connected with a sliding deflection frame (303) through bolts, a mounting clamping groove (304) is formed in the outer side of the sliding deflection frame (303), and a positioning connecting plate (308) is clamped on one side of the curved movable push plate (305);
water bath control mechanism (4) are including rivers storage frame (401), the inboard of rivers storage frame (401) is pegged graft and is had activity water pipe (402), one side of activity water pipe (402) is pegged graft and is had activity commentaries on classics valve (403), the both sides of activity commentaries on classics valve (403) are pegged graft and are had water injection frame (405), the outside rotation of activity commentaries on classics valve (403) is connected with bent shape runner handle (404), bent shape runner handle (404) joint is in the one side of location connecting plate (308), water injection frame (405) are the setting of water bath heating state through the deflection power of bent shape runner handle (404) with activity commentaries on classics valve (403).
2. The water bath shaker for extracting immunoglobulin from egg yolk according to claim 1, wherein: the bottom joint of water bath control mechanism (4) has water bath shaking table supporting mechanism (1), the inboard of water bath shaking table supporting mechanism (1) is provided with supplementary rocking mechanism (2), the supplementary inboard that rocks mechanism (2) rotates and is connected with immune ball and cultivates mechanism (5), one side of water bath control mechanism (4) joint at water bath guiding mechanism (3), the inboard of water bath guiding mechanism (3) rotates and is connected with heat preservation mechanism (6).
3. The water bath shaker for extracting immunoglobulin from egg yolk according to claim 2, wherein: water bath shaking table supporting mechanism (1) is including supporting frame spare (101), the inboard central weld that supports frame spare (101) has support card frame (102), the both sides joint that supports card frame (102) has spacing baffle plate frame (103), the outside of frame (401) is stored at rivers to spacing baffle plate frame (103) joint.
4. The water bath shaker for extracting immunoglobulin from egg yolk according to claim 2, wherein: supplementary mechanism (2) of rocking includes outside support frame (201), the outside of outside support frame (201) is provided with movable slide frame (205), the outside of outside support frame (201) has two-way activity card post (202) through movable slide frame (205) joint, the slide has been seted up to the inboard of two-way activity card post (202), the outside of two-way activity card post (202) has two slip card framves (203), two through the slide joint of two-way activity card post (202) the both sides of slip card frame (203) are pegged graft and are had flexible card pole (204).
5. The water bath shaker for extracting immunoglobulin from egg yolk according to claim 4, wherein: the immune ball cultivation mechanism (5) comprises an auxiliary cultivation frame (501), the number of the auxiliary cultivation frame (501) is multiple groups, the inner sides of the multiple groups of the auxiliary cultivation frame (501) are welded with partition plates (503), the inner sides of the multiple groups of the auxiliary cultivation frame (501) are inserted with multiple groups of clamping and clamping clamps (502), the inner sides of the clamping and clamping clamps (502) are inserted with threaded rotating rods (505), the two ends of the threaded rotating rods (505) are inserted with auxiliary receiving blocks (504), two sliding clamping frames (203) are inserted outside the auxiliary cultivation frame (501) through telescopic clamping rods (204), the multiple groups of clamping and clamping clamps (502) are arranged in a space adjusting state through the pulling of the threaded rotating rods (505), and the multiple groups of the auxiliary cultivation frame (501) are arranged in a shaking state through the synchronous deflection of the sliding clamping frame (203) and an external support frame (201), the auxiliary cultivation frame (501) is clamped outside the positioning clamping plate (302), and the surfaces of the auxiliary cultivation frames (501) are connected with telescopic rods in an inserting mode.
6. The water bath shaker for extracting immunoglobulin from egg yolk according to claim 2, wherein: the heat preservation mechanism (6) is including holding outer frame (601) by the card, the inboard of holding outer frame (601) is pegged graft and is had ring air bag frame (602), the outer surface of holding outer frame (601) by the card has seted up multiunit heat conduction hole, ring air bag frame (602) is the setting of expend with heat and contract with cold state through the heat conduction hole that holds outer frame (601) by the card, the inboard of ring air bag frame (602) is pegged graft and is had movable push rod (603).
7. The water bath shaker for extracting immunoglobulin from egg yolk according to claim 6, wherein: the heat preservation mechanism (6) further comprises an auxiliary central gear (608), a friction rotating wheel (605) is rotatably connected to the outer side of the auxiliary central gear (608), an air bag pushing frame (609) is arranged on the outer surface of the friction rotating wheel (605), a rubber containing push block is inserted into the inner side of the air bag pushing frame (609), a brake piston (604) is inserted into the outer side of the air bag pushing frame (609), a positioning movable push rod (607) is inserted into the inner side of the brake piston (604), torque spring frames (606) are arranged on two sides of the positioning movable push rod (607), and an electromagnetic movable frame (610) is inserted into the outer side of the torque spring frame (606).
8. The water bath shaker for extracting immunoglobulin from egg yolk according to claim 7, wherein: the utility model discloses a fixing device for card, including location activity push rod (607), electromagnetism adjustable shelf (610), the outside of electromagnetism adjustable shelf joint in activity push rod (603), the outside of friction runner (605) is provided with the connecting rod, friction runner (605) is in the outside of bent shape cardboard piece (301) through the connecting rod joint of friction runner (605), friction runner (605) is the setting of synchronous deflection state with bent shape cardboard piece (301).
9. A water bath shaking table debugging method for extracting immune globulin from egg yolk is characterized in that:
s1, respectively placing a temperature control module A and a vibration frequency monitoring module A at the liquid storage position of the water bath shaking table, and arranging a temperature control module B and a vibration frequency monitoring module B at the nearest position of the material placement;
s2, the temperature control module A, the vibration frequency monitoring module A, the temperature control module B and the vibration frequency monitoring module B carry out data debugging through a controller and a signal receiving module in the equipment;
s3, setting a proper temperature range of the material to be 45-65 ℃, and generating a text set of the proper temperature range;
s4, sequentially monitoring a temperature control module A and a temperature control module B, a vibration frequency monitoring module A and a vibration frequency monitoring module B in a temperature interval on a text set of a proper temperature interval, and generating a temperature difference text set and a vibration frequency difference text set;
calculating the absolute value of the difference between the temperature control module A and the temperature control module B according to different temperature nodes set by the heating equipment in the temperature difference text set;
calculating the absolute value of the difference between the vibration frequency monitoring module A and the vibration frequency monitoring module B by using different rotating speeds or vibration frequency nodes set by a motor of equipment generating vibration in the vibration frequency difference text set;
s5, selecting 3-5 temperature or rotating speed nodes or shaking frequency nodes with the minimum difference according to the temperature difference text set and the shaking frequency difference text set to generate an optimal temperature node text set and an optimal shaking frequency text set;
s6 selects and sets different values in the text set of the optimum temperature node and the text set of the optimum vibration frequency.
10. The method for adjusting immunoglobulin in egg yolk by using a water bath shaker as claimed in claim 9, wherein the method comprises the steps of: generating values for parameters of external factors in S6, and combining the values into a linear combination, wherein the external factors include temperature outside the equipment, humidity inside the equipment, humidity outside the equipment, and altitude of an area where the equipment is used;
linearly combining the randomly generated external factor parameters, then constructing a random forest by taking the random forest as an input variable, selecting N variables A, B, C, D.. Z and N random numbers O on each parameter node, linearly combining to obtain a variable Y,
Ni∈[-1,+1]
wherein i is a serial number, a corresponding variable is obtained according to the external factor parameter which is randomly generated, and the value of the external factor parameter T is randomly extracted,
T＝log2R
r is the total number of the external factor parameters of the input variables, a state that the variable Y is cracked downwards is formed by the value of T, finally, the corresponding variable Y is generated according to the parameters of different external factors, and the value of the text set corresponding to the optimal temperature node and the text set corresponding to the optimal vibration frequency is selected through the variable Y according to the preset value.
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| US5564826A (en) * | 1995-09-27 | 1996-10-15 | Robbins Scientific Corporation | Reciprocating bath shaker |
| CN106890686A (en) * | 2015-12-19 | 2017-06-27 | 西安瑞联新材料股份有限公司 | A kind of novel constant-temperature water-bath with shaking table |
| CN210632160U (en) * | 2019-08-06 | 2020-05-29 | 杭州市萧山区中医院 | Water bath constant temperature oscillator for polylactic acid-glycolic acid copolymer |
| CN211865471U (en) * | 2020-03-09 | 2020-11-06 | 尤丽康（江苏）生物医药有限公司 | Centrifugal device for extracting immune globulin from egg |
| CN215353178U (en) * | 2021-07-26 | 2021-12-31 | 江苏新春兰科学仪器有限公司 | Utilize circulating water to realize water bath constant temperature shaking table of accuse temperature |
-
2022
- 2022-02-25 CN CN202210177934.7A patent/CN114471772B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5564826A (en) * | 1995-09-27 | 1996-10-15 | Robbins Scientific Corporation | Reciprocating bath shaker |
| CN106890686A (en) * | 2015-12-19 | 2017-06-27 | 西安瑞联新材料股份有限公司 | A kind of novel constant-temperature water-bath with shaking table |
| CN210632160U (en) * | 2019-08-06 | 2020-05-29 | 杭州市萧山区中医院 | Water bath constant temperature oscillator for polylactic acid-glycolic acid copolymer |
| CN211865471U (en) * | 2020-03-09 | 2020-11-06 | 尤丽康（江苏）生物医药有限公司 | Centrifugal device for extracting immune globulin from egg |
| CN215353178U (en) * | 2021-07-26 | 2021-12-31 | 江苏新春兰科学仪器有限公司 | Utilize circulating water to realize water bath constant temperature shaking table of accuse temperature |
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