CN114470859A

CN114470859A - Improved Shangshu Yilao prescription and water extraction process thereof

Info

Publication number: CN114470859A
Application number: CN202210118443.5A
Authority: CN
Inventors: 方彦雯; 方志财
Original assignee: Heye Health Technology Co Ltd
Current assignee: Heye Health Technology Co Ltd
Priority date: 2022-02-08
Filing date: 2022-02-08
Publication date: 2022-05-13

Abstract

The invention relates to the field of medicinal and edible foods, in particular to an improved Shangshu Yilao prescription and a water extraction process thereof. The paint comprises the following components in parts by weight: 25-35 parts of lotus seed, 10-20 parts of dried medlar, 10-20 parts of cornus officinalis, 10-20 parts of polygonatum kingianum and 10-20 parts of gordon euryale, mixing, adding 10-15 times of water by volume, soaking for 1-3h, extracting for 15-20min, and extracting for 2-4 times. The method removes Chinese yam and tuckahoe in the old prescription, and adds polygonatum rhizome which is prepared by nine times of steaming and nine times of drying in the sun wine on the basis; the paste formula food which has higher paste yield and total flavone content, more stable quality and convenient carrying and taking can be obtained by the water extraction process. Different dosage forms can be prepared by optimizing the water extraction process, the product form is enriched, and the carrying and the taking are convenient.

Description

Improved Shangshu Yilao prescription and water extraction process thereof

Technical Field

The invention relates to the field of medicinal and edible foods, in particular to an improved Shangshu Yilao prescription and a water extraction process thereof.

Background

The Shangshu Yilao prescription is obtained by adding and subtracting Yang strengthening pills according to Zhonghu Shangshu Zhuyang pill from Fushou Jing Fang, and mainly aims at the middle-aged and the elderly and has the effects of nourishing the original Yang, beautifying the face, prolonging the life and the like. The original recipe comprises semen Nelumbinis, fructus Lycii, semen euryales, dried rhizoma Dioscoreae, Poria, and Corni fructus; the Chinese yam is sweet in taste and neutral in nature, enters spleen, lung and kidney channels, has the effects of tonifying qi and yin, tonifying spleen and lung, filling acquired qi, growing muscle and stretching tendon, and nourishing skin, and is matched with the lotus seeds to tonify kidney and arrest spontaneous emission, so that spleen and kidney are regulated together to help health and longevity. Poria has sweet and light taste, neutral nature, and effects of benefiting heart, spleen and kidney channels, promoting diuresis, eliminating dampness, calming heart and invigorating spleen. However, the traditional decoction has certain limitations, and has the problems of unstable quality, difficult carrying and taking and the like.

Disclosure of Invention

One of the objectives of the present invention is to solve the above technical problems and to provide an improved book-saving and old-fashioned book-building.

The second purpose of the invention is to solve the technical problems and provide an improved water extraction process for the book-growing old-fashioned prescription.

The first technical purpose of the invention is realized by the following technical scheme:

an improved book-saving old-fashioned prescription comprises the following components in parts by weight: 25-35 parts of lotus seed, 10-20 parts of dried medlar, 10-20 parts of cornus officinalis, 10-20 parts of polygonatum sibiricum processed with wine and 10-20 parts of gordon euryale seed.

The traditional Chinese medicine is sweet and neutral, mainly tonifies spleen, lung and kidney, treats diseases by 'nourishing' instead of 'treating diseases' in the whole formula, and strengthens the foundation and strengthens the primordial qi instead of attacking the purgation. The lotus seed is the monarch drug with the largest dosage, is lotus meat with peeled cores, reduces bitter function, is mainly sweet and tonifying, enters spleen and kidney channels, has the functions of tonifying spleen and stomach, securing essence and qi and strengthening tendons and bones, and is most suitable for the old with deficiency. The medlar is sweet in taste and neutral in nature, enters liver and kidney channels, has the effects of nourishing liver and kidney, replenishing vital essence and improving eyesight, and is supplemented with lotus seed for regulating qi. The gorgon fruit is sweet and astringent in taste and neutral in nature, and has the effects of tonifying kidney, securing essence, tonifying spleen, stopping diarrhea and the like, and spleen and kidney meridians entered. Cornus officinalis is sour and astringent in taste and slightly warm in nature, enters liver and kidney meridians, has the effects of tonifying liver and kidney and arresting seminal emission, is a medicinal and edible medicinal material, warms kidney yang, astringes kidney essence, nourishes liver and kidney, promotes the biochemistry of menstrual blood, and tonifies without stagnation, and is matched with medlar to regulate liver and kidney. The medicines are used together to nourish the primordial yang and beautify the face and prolong the life.

The inventor finds that the rhizoma dioscoreae and poria cocos in the old formula are removed, and the rhizoma polygonati which is processed by steaming and sun-drying the wine is added on the basis, so that the effects of reinforcing the spleen and tonifying the lung and tonifying qi and yin are enhanced, the rhizoma polygonati is clinically and commonly used for treating symptoms such as deficiency of spleen and stomach qi, tiredness and weakness, dry mouth and poor appetite, lung deficiency and cough and dryness, and can also be used for treating symptoms such as soreness and weakness of waist and knees, early white beard and hair and the like, the processed rhizoma polygonati is removed of pungent dryness, and the wine can be used for guiding medicine upwards, assisting in medicine vigor and enhancing the tonifying function, is suitable for the old with asthenia and weakness, can enhance the anti-fatigue capability and has the function of stabilizing blood sugar; the paste formula food which has higher paste yield and total flavone content, more stable quality and convenient carrying and taking can be obtained by the water extraction process.

Preferably, the improved Shangshu Yilao prescription comprises the following components in parts by weight: 30 parts of lotus seed, 15 parts of dried medlar, 15 parts of cornus officinalis, 15 parts of wine sealwort and 15 parts of gordon euryale seed.

The second technical purpose of the invention is realized by the following technical scheme:

an improved water extraction process of Shangshu Yilao prescription comprises mixing lotus seed 25-35 parts, dried medlar 10-20 parts, pulp of dogwood fruit 10-20 parts, alcohol-processed rhizoma polygonati 10-20 parts and gorgon fruit 10-20 parts according to the weight parts, adding 10-15 times of water for soaking for 1-3h, extracting for 15-20min, and extracting for 2-4 times.

The inventor adjusts the water extraction process on the basis of the formula to obtain the ointment formula with higher ointment yield and total flavone content and more stable quality. The inventor finds that the extraction times have the greatest influence on the comprehensive score of the water extraction process of the Shangshu Yilao prescription, the water addition amount is used secondly, and the extraction time is used thirdly. The obtained ointment yield is more than 37%, and the content of total flavonoids is more than 1.36 mg/ml. The medicinal materials used in the formula all belong to medicine-food homologous traditional Chinese medicines, a Box-Behnken response surface method is adopted, the cream yield and the total flavone content are used as evaluation indexes, different dosage forms can be prepared through water extraction process optimization, the product form is enriched, and the carrying and the taking are convenient.

Preferably, the paste preparation device used in the water extraction process comprises a concentration tank, wherein a foam collecting box is welded at the top of one side of the outer part of the concentration tank, a communication port is horizontally formed between the concentration tank and the foam collecting box, an L-shaped mounting frame is horizontally inserted into the foam collecting box, a filter screen is horizontally embedded into the L-shaped mounting frame, the bottom of the foam collecting box is communicated with the concentration tank through a communication pipe, a push plate is horizontally arranged above one side of the inner part of the concentration tank, a moving mechanism for driving the push plate to move is arranged on the outer side of the concentration tank, a mounting box is horizontally welded above one side of the inner part of the concentration tank, one end of the bottom of the mounting box is vertically and rotatably connected with a driven shaft, a driving mechanism for driving the driven shaft to rotate is arranged in the mounting box, sleeves are horizontally welded at the top and the bottom of the periphery of the driven shaft, and telescopic rods are inserted into the two sleeves, the telescopic rods are connected with the sleeve in a sliding manner, a spring is horizontally welded between one end of each telescopic rod and the inner wall of the sleeve, a stirring rod is vertically welded between the two telescopic rods, and stirring blades are horizontally arranged in the middle of the periphery of the driven shaft; and a check valve is arranged in the communicating pipe, and the push plate and the communicating port are arranged in parallel and have the same size.

The improved paste preparation device for the water extraction process of the Shangshu Yilao prescription has the following beneficial effects:

1. by arranging the push plate and the froth collecting box, when the cream stock solution is concentrated in the concentrating tank to generate froth, the electric telescopic rod is started to drive the push plate to move forwards so that the push plate can slowly push the froth on the surface of the liquid to the communicating port, and the froth enters the froth collecting box from the communicating port to wait for uniform cleaning, so that the froth can be automatically skimmed, manual operation is not needed, time and labor are saved, workers are prevented from being scalded by steam, and the paste concentrate concentrating tank is safer; the stirring blade, the sleeve, the telescopic rod and the stirring rod are arranged, the driven shaft drives the stirring blade when rotating, the sleeve and the telescopic rod rotate to stir the paste side stock solution, the telescopic rod drives the stirring rod to rotate to stir the paste side stock solution, meanwhile, the rotation of the driven shaft provides centrifugal force for the telescopic rod, so that the telescopic rod is thrown out of the sleeve for a certain distance from the inside of the sleeve, the telescopic rod stretches out and stretches the spring, the spring generates pulling force to pull the telescopic rod back to the inside of the sleeve, the telescopic rod is repeatedly stretched out and retracted in the sleeve in a reciprocating mode, the stirring rod moves horizontally while rotating, the paste side stock solution forms turbulent flow, and the stirring effect is better;

2. through being provided with filter screen and communicating pipe, fall on the filter screen surface after floating the foam entering collection foam case, when partial cream side stoste gets into collection foam case along with floating the foam, float the foam and be held back by the filter screen, only get rid of pure superficial foam, cream side stoste gets back to concentrated jar inside once more through communicating pipe through the filter screen to prevent that cream side stoste from producing extravagantly.

Preferably, the top center department of concentrated jar has seted up the feed inlet, one side bottom intercommunication of concentrated jar has the discharging pipe, and installs the valve on the discharging pipe, one side mid-mounting of concentrated jar has the control box.

Preferably, collection foam case and L type mounting bracket hookup location bond and have sealed the pad, L type mounting bracket horizontal part's tip is inlayed and is had first magnet, collection foam incasement wall has seted up the slot, and the slot is inside to be inlayed and to have the second magnet, first magnet and second magnet magnetism attract mutually.

Preferably, moving mechanism includes electric telescopic handle, electric telescopic handle horizontal installation is in concentrated jar outside one side top, electric telescopic handle's flexible end stretch into concentrated jar inside and with concentrated jar sliding connection, electric telescopic handle's flexible end and the one side middle part fixed connection of push pedal.

Preferably, actuating mechanism includes the rotating electrical machines, rotating electrical machines horizontal installation is in the top in concentrated jar outside one side, the output shaft of rotating electrical machines stretches into the mounting box inside and rotates with concentrated jar to be connected, the output shaft horizontal fixed mounting of rotating electrical machines has the driving shaft.

Preferably, a driving bevel gear is installed at the top end of the driving shaft, the top end of the driven shaft extends into the installation box, a driven bevel gear is installed at the top of the driven shaft, and the driving bevel gear is meshed with the driven bevel gear.

Preferably, the sleeve is equipped with a plurality ofly, and is a plurality of the sleeve is circumference evenly distributed at the top and the bottom of periphery, the stirring leaf is equipped with a plurality ofly, and is a plurality of the stirring leaf is at the middle part evenly distributed of driven shaft periphery.

Preferably, the extraction process comprises adding 12 times of water, soaking for 2 hr, extracting for 18min, and extracting for 3 times.

Drawings

FIG. 1 is a schematic diagram of the interaction between A (water addition) and B (extraction time) according to the present invention;

FIG. 2 is a schematic diagram showing the interaction between A (amount of water added) and C (number of extractions) according to the present invention;

FIG. 3 is a schematic diagram of the interaction between B (extraction time) and C (extraction times) according to the present invention;

FIG. 4 is an overall top cross-sectional view of a paste formulation preparation apparatus for use in the water extraction process of the still-known senior formulation of the present invention;

FIG. 5 is a schematic view of the entire exterior of a paste formulation preparation apparatus used in the water extraction process of Shangshi Lao Fang of the present invention;

FIG. 6 is a sectional view of a paste preparation apparatus for use in the aqueous extraction process of the still-to-be-read old-fashioned prescription of the present invention;

FIG. 7 is an enlarged view taken at A of FIG. 6 according to the present invention.

In the figure: 1. a communication port; 2. mounting a box; 3. pushing the plate; 4. an electric telescopic rod; 5. a rotating electric machine; 6. a concentration tank; 7. a sleeve; 8. a telescopic rod; 9. a foam collecting box; 10. stirring blades; 11. a spring; 12. a driven shaft; 13. a drive shaft; 14. a drive bevel gear; 15. a driven bevel gear; 16. a stirring rod; 17. a communicating pipe; 18. a non-return valve; 19. filtering with a screen; 20. a first magnet; 21. a second magnet; 22. a gasket; 23. an L-shaped mounting rack.

Detailed Description

Example 1

An improved water extraction process of a Shangshu Yilao prescription comprises the steps of proportioning 25 parts of lotus seed, 20 parts of dried medlar, 20 parts of cornus officinalis, 20 parts of wine sealwort and 20 parts of gordon euryale seed according to the parts by weight, adding 15 times of volume of water, soaking for 3 hours, extracting for 20 minutes, and extracting for 4 times.

Example 2

An improved water extraction process of a Shangshu Yilao prescription comprises the steps of proportioning 35 parts of lotus seed, 10 parts of dried medlar, 10 parts of cornus officinalis, 10 parts of alcohol-processed rhizoma polygonati and 10 parts of gordon euryale seed according to the parts by weight, adding 10 times of water by volume, soaking for 1 hour, extracting for 15min, and extracting for 2 times.

Example 3

An improved water extraction process of a Shangshu Yilao prescription comprises the steps of proportioning 30 parts of lotus seed, 15 parts of dried medlar, 15 parts of cornus officinalis, 15 parts of alcohol-processed rhizoma polygonati and 15 parts of gordon euryale seed according to the parts by weight, adding 12 times of water by volume, soaking for 2 hours, extracting for 18 minutes, and extracting for 3 times.

Example 4

The difference from embodiment 3 is that the improved device for the water extraction process of the Shangshu Yilao formulation adopts the specific paste formulation preparation device of the invention, as shown in fig. 4-7, the device comprises a concentration tank 6, a heating system is arranged in the concentration tank 6, a foam collecting tank 9 is welded at the top of one side outside the concentration tank 6, a communication port 1 is horizontally arranged between the concentration tank 6 and the foam collecting tank 9, an L-shaped mounting rack 23 is horizontally inserted in the foam collecting tank 9, a filter screen 19 is horizontally embedded in the L-shaped mounting rack 23, the bottom of the foam collecting tank 9 is communicated with the concentration tank 6 through a communication pipe 17, when floating foam enters the foam collecting tank 9, the floating foam falls on the surface of the filter screen 19, when partial paste formulation solution enters the foam collecting tank 9 along with the floating foam, the floating foam is intercepted by the filter screen 19, the paste formulation solution returns to the interior of the concentration tank 6 through the filter screen 19 again through the communication pipe 17, thereby preventing the waste of the paste formulation solution and only removing pure floating foam, a push plate 3 is horizontally arranged above one side inside a concentration tank 6, a moving mechanism for driving the push plate 3 to move is arranged outside the concentration tank 6, a mounting box 2 is horizontally welded above the middle of one side inside the concentration tank 6, one end of the bottom of the mounting box 2 is vertically and rotatably connected with a driven shaft 12, a driving mechanism for driving the driven shaft 12 to rotate is arranged in the mounting box 2, sleeves 7 are horizontally welded at the top and the bottom of the periphery of the driven shaft 12, telescopic rods 8 are inserted in the two sleeves 7, the telescopic rods 8 are slidably connected with the sleeves 7, a spring 11 is horizontally welded between one end of each telescopic rod 8 and the inner wall of each sleeve 7, a stirring rod 16 is vertically welded between the two telescopic rods 8, stirring blades 10 are horizontally arranged in the middle of the periphery of the driven shaft 12, the telescopic rods 8 drive the stirring rods 16 to rotate to stir paste stock solution, meanwhile, the rotation of the driven shaft 12 provides a centrifugal force for the telescopic rods 8, and accordingly, one distance is thrown out of the telescopic rods 8 from the insides of the sleeves 7, extension spring 11 after telescopic link 8 stretches out, inside spring 11 produced the pulling force and pull back sleeve 7 with telescopic link 8, so reciprocal messenger telescopic link 8 is at the inside reciprocal withdrawal that stretches out of sleeve 7 to make 16 limits of puddler rotate the limit horizontal to removing, make cream side stoste form the turbulent flow, stirring effect is better.

In this embodiment, as shown in fig. 4-6, a feed inlet has been seted up at the top center of concentration tank 6, and one side bottom intercommunication of concentration tank 6 has the discharging pipe, and installs the valve on the discharging pipe, and one side mid-mounting of concentration tank 6 has the control box, adds the cream side stoste into concentration tank 6 from the feed inlet and concentrates, opens the valve after the concentration and follows the discharging pipe ejection of compact, and each power equipment is controlled to the control box.

In this embodiment, as shown in fig. 6 to 7, a sealing gasket 22 is bonded to a connection position of the foam collecting box 9 and the L-shaped mounting bracket 23, the sealing gasket 22 prevents liquid leakage, a first magnet 20 is inlaid in an end portion of a horizontal portion of the L-shaped mounting bracket 23, a slot is formed in an inner wall of the foam collecting box 9, a second magnet 21 is inlaid in the slot, the first magnet 20 and the second magnet 21 attract each other magnetically, and the first magnet 20 and the second magnet 21 attract each other to fix the L-shaped mounting bracket 23 in the foam collecting box 9.

In this embodiment, as shown in fig. 4, 6 and 7, a check valve 18 is installed in the communicating pipe 17, the push plate 3 and the communicating port 1 are arranged in parallel and have the same size, and the check valve 18 prevents the liquid inside the concentration tank 6 from entering the foam collecting tank 9.

In this embodiment, as shown in fig. 4 and 6, moving mechanism includes electric telescopic handle 4, 4 horizontal installation of electric telescopic handle is in concentrated 6 outside one sides tops of jar, electric telescopic handle 4 flexible end stretch into concentrated 6 inside and with concentrated 6 sliding connection of jar, electric telescopic handle 4 flexible end and one side middle part fixed connection of push pedal 3, when concentrated production froth, start electric telescopic handle 4 and drive push pedal 3 and move forward and make push pedal 3 slowly promote the froth on liquid surface to intercommunication mouth 1, it is unified to wait for the clearance in the foam collecting box 9 to get into from intercommunication mouth 1, thereby can skim the froth automatically, need not manual operation, time saving and labor saving, and avoid vapor scald the staff, and is comparatively safe.

In this embodiment, as shown in fig. 4 and 6, the driving mechanism includes a rotating electrical machine 5, the rotating electrical machine 5 is horizontally installed in the upper portion of one side outside the concentration tank 6, the output shaft of the rotating electrical machine 5 extends into the mounting box 2 and is rotatably connected with the concentration tank 6, a driving shaft 13 is horizontally and fixedly installed on the output shaft of the rotating electrical machine 5, and the rotating electrical machine 5 is started to drive the driving shaft 13 to rotate.

In this embodiment, as shown in fig. 6, a driving bevel gear 14 is installed at the top end of the driving shaft 13, the top end of the driven shaft 12 extends into the mounting box 2, a driven bevel gear 15 is installed at the top end of the driven shaft 12, the driving bevel gear 14 is engaged with the driven bevel gear 15, the driving shaft 13 drives the driving bevel gear 14 to rotate, and the driving bevel gear 14 drives the driven shaft 12 to rotate through the driven bevel gear 15.

In this embodiment, as shown in fig. 6, sleeve 7 is equipped with a plurality ofly, and a plurality of sleeves 7 are circumference evenly distributed at the top and the bottom of periphery, and stirring leaf 10 is equipped with a plurality ofly, and a plurality of stirring leaves 10 are at the middle part evenly distributed of driven shaft 12 periphery, and driven shaft 12 drives stirring leaf 10, sleeve 7 and telescopic link 8 when rotating and rotates and stir the cream side stoste.

The working principle of the invention is as follows: adding the paste stock solution into a concentration tank 6 from a feed inlet for concentration, starting a rotating motor 5 to drive a driving shaft 13 to rotate, driving the driving shaft 13 to drive a driving bevel gear 14 to rotate, driving the driving bevel gear 14 to drive a driven shaft 12 to rotate through a driven bevel gear 15, driving a stirring blade 10, a sleeve 7 and a telescopic rod 8 to stir the paste stock solution when the driven shaft 12 rotates, driving a stirring rod 16 to rotate by the telescopic rod 8 to stir the paste stock solution, and simultaneously, the rotation of the driven shaft 12 gives a centrifugal force to the telescopic rod 8, so that the telescopic rod 8 is thrown out a certain distance from the interior of the sleeve 7, the spring 11 is stretched after the telescopic rod 8 is stretched out, the spring 11 generates pulling force to pull the telescopic rod 8 back to the interior of the sleeve 7, the telescopic rod 8 is repeatedly stretched out and retracted in the interior of the sleeve 7 in such a reciprocating way, thereby enabling the stirring rod 16 to horizontally move while rotating, enabling the paste stock solution to form turbulent flow and achieving better stirring effect; when froth is generated by concentration, the electric telescopic rod 4 is started to drive the push plate 3 to move forwards, so that the push plate 3 slowly pushes the froth on the surface of the liquid to the communication port 1 forwards, and the froth enters the froth collecting box 9 from the communication port 1 to wait for uniform cleaning, so that the froth can be skimmed automatically, manual operation is not needed, time and labor are saved, workers are prevented from being scalded by steam, and the device is safe; drop on 19 surfaces of filter screen after floating the foam entering collection foam case 9, when partial cream side stoste got into collection foam case 9 along with floating the foam together, float the foam and be held back by filter screen 19, and cream side stoste gets back to concentration jar 6 inside once more through communicating pipe 17 through filter screen 19 to prevent that cream side stoste from producing extravagantly, only get rid of the pure foam that floats.

Detection experiment:

1 materials

1.1 instruments

Ultraviolet-visible spectrophotometer, electromagnetic oven, and constant temperature water bath

1.2 reagent

Lotus seed, medlar, dogwood fruit, polygonatum sibiricum wine, gordon euryale seed and dogwood fruit are purchased from the Anguo medicinal material market, rutin standard products and analytical grade methanol.

2 method

2.1 Single factor experiment

The optimal extraction scheme is comprehensively evaluated by taking the cream yield and the total flavone content as indexes.

Three factors of water addition, extraction time and extraction times are investigated, and the following is a specific method step of a single-factor investigation experiment.

Investigation of the amount of water added: weighing 30g of lotus seed meat, 15g of dried medlar, 15g of cornus officinalis, 15g of rhizoma polygonati preparata and 15g of gordon euryale seed, respectively adding water with volume of 8 times, 10 times and 12 times of the lotus seed meat, soaking for 2 hours, extracting for 10min and extracting for 2 times.

Examination of extraction time: weighing 30g of lotus seed meat, 15g of dried medlar, 15g of cornus officinalis, 15g of rhizoma polygonati preparata and 15g of gordon euryale seed, adding 10 times of water by volume, soaking for 2 hours, and extracting for 10min, 20min and 30min respectively for 2 times.

Examination of extraction frequency: weighing 30g of lotus pulp, 15g of dried medlar, 15g of cornus officinalis, 15g of rhizoma polygonati preparata and 15g of gordon euryale seed, adding 10 times of water by volume, soaking for 2h, extracting for 20min, and extracting for 1, 2 and 3 times respectively.

2.2 Box-Behnken response surface method design experiment

According to a single-factor experiment, 3 factors of water addition amount (A), extraction time (B) and extraction times (C) are selected as objects to be examined, and each factor has three levels, as shown in table 1:

TABLE 1 level table of experimental design factors of response surface

Designing an experimental scheme by adopting a Box-Behnken response surface method in Design Expert 11, wherein 15 groups are designed in total, and Design tables and results are shown in a table 2;

2.3 determination of cream yield

The method for measuring the cream yield comprises the following steps: precisely absorbing 20ml of sample extract, adding into an evaporating dish dried to constant weight, evaporating in water bath, drying in a 105 ℃ oven for 3h, transferring into a drier, cooling for 1h to room temperature, rapidly and precisely weighing the mass, measuring the mass of the dried product by adopting a weight reduction method, and calculating the cream yield according to the following formula (W is the cream mass, M is the medicinal material mass, and V is the volume of the sample solution)

The cream yield (%) = (W × V)/(20 × M) × 100%

2.4 calculation of composite score

Taking the cream yield and the total flavone content as indexes, adopting a weighted comprehensive scoring method, wherein the weight ratio of the cream yield to the total flavone content is 50 and 50 respectively, and calculating the comprehensive scoring according to the following formula:

composite score = (total flavone content/maximum total flavone content + cream yield/maximum cream yield) × 0.5

2.5 determination of Total Flavonoids content

2.5.1 preparation and content determination of Standard substance solution

Precisely weighing 1.7mg of rutin standard substance in a 10ml volumetric flask, and fixing the volume (the concentration is 0.17 mg/ml) by using methanol; respectively taking 0.1ml, 0.2ml, 0.4ml, 0.8ml, 1.6ml and 3.2ml of the solution in a 10ml volumetric flask, and fixing the volume by using methanol; measuring absorbance at 360nm, and drawing a standard curve;

2.5.2 preparation and content determination of test solution

Precisely weighing 10mg of a sample volatilized in a water bath, adding 10ml of methanol, carrying out ultrasonic treatment for 10 minutes, centrifuging for 1000r, carrying out 5min, taking 1ml of supernate, putting the supernate into a 10ml volumetric flask, carrying out constant volume treatment by using the methanol, and measuring the absorbance at 360 nm.

3 results of

3.1 drawing of rutin Standard Curve

Drawing a standard curve according to the absorbance values of rutin with different concentrations measured at 360nm to obtain an equation of the absorbance and the concentration, namely y =1.4346x-0.0217, R²=0.9987, the linear relation is good, and the concentration of the sample can be calculated according to the formula and the measured absorbance of the sample;

3.2 Box-Behnken response surface method design experiment scale

Calculating to obtain total flavone content according to the extract yield formula and rutin standard curve, and obtaining comprehensive score according to the formula, wherein the result is shown in Table 2:

TABLE 2 response surface design and results

3.3 Box-Behnken response surface method analysis

The composite scores were subjected to multiple linear regression using software Design Expert 11, with results as given in table 3:

TABLE 3 regression model analysis of variance

The regression equation was found to be Y (composite score) =72.37+ 7.04A-4.65B + 18.12C +0.47 AB +1.51 AC-5.03 BC-0.46A²-1.33*B²-4.52*C²， R²And = 0.995. The P values of the interaction items AB and AC are both greater than 0.05, which indicates that the interaction between A and B is weak, the P value between B and C is less than 0.05, and indicates that the interaction between B and C is certain; A. b, C, wherein P values are all less than 0.05, the influence of A, B, C on comprehensive scores is obvious, wherein P (B) > P (A) > P (C), C (extraction times) has the largest influence on comprehensive scores of the water extraction process of the book-losing and old-fashioned square, A (water addition amount) is used, B (extraction time) has the smallest influence on comprehensive scores, and finally, the influence results of 3 factor levels on the extraction process of the book-losing and old-fashioned square are sequentially C (extraction times) > A (water addition amount) > B (extraction time), a regression model P < 0.0001, the model is obvious, r =0.995, the model fitting degree is good, the mismatching item is greater than 0.05, the model mismatching is not significant, the experimental error is small, and the optimal extraction process of the book-losing and old-fashioned square can be well predicted.

In order to more intuitively see the interaction influence of various factors on the comprehensive scoring of the water extraction process of the old-fashioned prescription, a response surface graph and a contour graph are drawn through software Design Expert 11, and are shown as fig. 1, fig. 2 and fig. 3.

As can be seen from the response surface graph and the contour graph, when one factor is fixed, the influence of the other two factors on the comprehensive score is fixed. As shown in FIG. 1, when C (number of extractions) is fixed, A (amount of water addition) and B (extraction time) are nearly in the same plane, and contour lines are nearly parallel, which indicates that the interaction between A and B is weak; as shown in fig. 2, when B (extraction time) is fixed, when both a (water addition amount) and C (extraction times) are close to the maximum value, the composite score is high; from fig. 3, it can be seen that when a (water addition amount) is fixed, B (extraction time) is small, and C (extraction frequency) is large, the composite score is high. In conclusion, while the influence of the comprehensive factors of the three is considered, the optimal water extraction scheme obtained by software is that the water addition amount is 12 times, the extraction time is 17.638 minutes, the extraction times are 3 times, the obtained paste yield is 37.53%, the total flavone content is 1.367mg/ml, and the comprehensive score is 96.16 highest. In combination, the optimal extraction process was determined to be 12 times the amount of water added, soaking for 2 hours followed by extraction for 18 minutes for a total of 3 times.

Meanwhile, the inventor finds that the device used in the improved water extraction process of the Shangshu Yilao formula in the example 4 adopts the specific device for preparing the paste formula to obtain the paste yield of 38.37 percent and the total flavone content of 1.396mg/ml, and the quality of the paste is better than that of the paste prepared in the examples 1-3.

Comparative example 1

The same as example 1, except that it did not contain polygonatum kingianum, and the number of extraction was 1. Through detection, the cream yield is 28.85%, and the content of the total flavonoids is 1.116 mg/ml.

Comparative example 2

Similar to example 2, except that 40g of lotus seed, 8g of dried medlar, 8g of cornus officinalis, 8g of alcohol solomonseal rhizome and 8g of gordon euryale seed are added, and then water with volume of 8 times is added for soaking for 0.5h and extraction is carried out for 10 min. Detection shows that the cream yield is 31.16% and the content of total flavonoids is 1.203 mg/ml.

Test example 1 acute toxicity test

Test materials:

animals: 70 mice are taken, each mouse weighs 18 g-22 g, and the male and female halves are respectively.

Medicine preparation: the invention relates to a pulverized traditional Chinese medicine composition powder.

Mice were randomly divided into 7 groups of 10 mice each, each half male and female. The cream formulation was adjusted to be pasty (maximum concentration is standard), each mouse was perfused with a maximum amount of the drug administered twice a day for 14 consecutive days and the animals were observed. In the observation period of 14 days, the mice were normal in appetite, activity, hair color, mental status, etc., developed normally, and had no death. The medicine of the present invention has high safety, no obvious side effect and very low acute toxicity.

Test example 2 drug efficacy test

The influence of the traditional Chinese medicine composition on the mouse cough model is as follows:

the mice were randomly divided into a model control group, and a blank control group, an example group and a comparative example group were set. The administration is carried out by intragastric administration for 5 days every day, and after the last administration for 2h, the mice are placed in a bell jar, and concentrated ammonia water is uniformly sprayed for 5s to stimulate the mice to cause cough, and the cough frequency of the mice within 10min is observed.

Table 1 effect of examples, comparative examples and control on mouse cough model

The experimental result shows that compared with the original old formula control group, the components of the formula of the ointment prepared from the raw materials have an inhibiting effect on the cough caused by strong ammonia water, but the formula of the traditional Chinese medicine composition in the examples 1 to 4 has a more obvious inhibiting effect on the cough caused by strong ammonia water, the effects of the examples 1 to 4 are better than those of the comparative examples 1 to 2, wherein the effect of the example 3 is better than those of the examples 1 and 2, and the effect of the example 4 is better than that of the example 3.

Test example 3 clinical test

General materials: 140 adult patients with spleen-lung qi deficiency diagnosed by traditional Chinese medicine diagnosis in recent two years are selected as study objects, 80 young people of 18-40 years old are selected, 40 male people and 40 female people are selected, 60 middle-aged people of 41-60 years old are selected, and 30 male people and 30 female people are selected.

TABLE 2 change in clinical efficacy after treatment of examples, comparative examples and control group

As can be seen from the above table, the cream formula components formulated from the raw materials of the present invention have better effects on the cure rate and the significant therapeutic effect compared to the original old formula control group and comparative examples 1, 2, wherein the effects of examples 1-4 are better than those of comparative examples 1-2, wherein the effect of example 3 is better than those of examples 1 and 2, and the effect of example 4 is better than that of example 3.

The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.

Claims

1. An improved book-keeping and old-age benefitting prescription is characterized in that: the paint comprises the following components in parts by weight: 25-35 parts of lotus pulp, 10-20 parts of dried medlar, 10-20 parts of cornus officinalis, 10-20 parts of polygonatum kingianum and 10-20 parts of gordon euryale seed.

2. An improved book protection benefit according to claim 1, wherein: the paint comprises the following components in parts by weight: 30 parts of lotus pulp, 15 parts of dried medlar, 15 parts of cornus officinalis, 15 parts of rhizoma polygonati preparata and 15 parts of gordon euryale seed.

3. An improved water extraction process for a Shangshu Yilao prescription is characterized in that: comprises 25-35 parts of lotus seed, 10-20 parts of dried medlar, 10-20 parts of dogwood fruit, 10-20 parts of alcohol solomonseal rhizome and 10-20 parts of gordon euryale seed according to the weight parts, and then water with the volume of 10-15 times is added for soaking for 1-3h, extraction is carried out for 15-20min, and extraction is carried out for 2-4 times.

4. The improved aqueous extraction process of the book-growing old-fashioned prescription according to claim 3, wherein: the cream formula preparation facilities that water extraction technology used includes concentrated jar (6), the top welding of concentrated jar (6) outside one side has foam collecting box (9), intercommunication mouth (1) has been seted up to the level between concentrated jar (6) and foam collecting box (9), the level is pegged graft in foam collecting box (9) inside has L type mounting bracket (23), L type mounting bracket (23) inside level is inlayed and is had filter screen (19), communicate through communicating pipe (17) between foam collecting box (9) bottom and the concentrated jar (6), the top level of one side in concentrated jar (6) inside is provided with push pedal (3), the concentrated jar (6) outside is equipped with the moving mechanism that drives push pedal (3) and remove, the horizontal welding of top in one side in concentrated jar (6) inside has mounting box (2), the vertical rotation of mounting box (2) bottom one end is connected with driven shaft (12), a driving mechanism for driving the driven shaft (12) to rotate is arranged in the mounting box (2), sleeves (7) are horizontally welded at the top and the bottom of the periphery of the driven shaft (12), telescopic rods (8) are inserted in the two sleeves (7), the telescopic rods (8) are connected with the sleeves (7) in a sliding manner, a spring (11) is horizontally welded between one end of each telescopic rod (8) and the inner wall of each sleeve (7), a stirring rod (16) is vertically welded between the two telescopic rods (8), and stirring blades (10) are horizontally arranged in the middle of the periphery of the driven shaft (12); a check valve (18) is installed in the communicating pipe (17), and the push plate (3) and the communicating opening (1) are arranged in parallel and are consistent in size.

5. The improved aqueous extraction process of the book-growing old-fashioned prescription according to claim 4, wherein: moving mechanism includes electric telescopic handle (4), electric telescopic handle (4) horizontal installation is in concentrated jar (6) outside one side top, the flexible end of electric telescopic handle (4) stretch into concentrated jar (6) inside and with concentrated jar (6) sliding connection, the flexible end of electric telescopic handle (4) and the one side middle part fixed connection of push pedal (3).

6. The improved aqueous extraction process of the book-growing old-fashioned prescription according to claim 5, wherein: the driving mechanism comprises a rotating motor (5), the rotating motor (5) is horizontally installed above the middle portion of one side of the outside of the concentration tank (6), an output shaft of the rotating motor (5) stretches into the mounting box (2) and is connected with the concentration tank (6) in a rotating mode, and a driving shaft (13) is horizontally and fixedly installed on the output shaft of the rotating motor (5).

7. The improved aqueous extraction process of the book-growing old-fashioned prescription according to claim 6, wherein: the top of driving shaft (13) is installed initiative bevel gear (14), inside the top of driven shaft (12) stretched into mounting box (2), driven bevel gear (15) were installed at the top of driven shaft (12), initiative bevel gear (14) and driven bevel gear (15) meshing.

8. The improved aqueous extraction process of the book-growing old-fashioned prescription according to claim 7, wherein: the feed inlet has been seted up to the top center department of concentrated jar (6), one side bottom intercommunication of concentrated jar (6) has the discharging pipe, and installs the valve on the discharging pipe, one side mid-mounting of concentrated jar (6) has the control box.

9. The improved aqueous extraction process of the book-growing old-fashioned prescription according to claim 8, wherein: collection foam case (9) and L type mounting bracket (23) hookup location bond and have sealed pad (22), the tip of L type mounting bracket (23) horizontal part is inlayed and is had first magnet (20), the slot has been seted up to collection foam case (9) inner wall, and the inside second magnet (21) of inlaying of slot, first magnet (20) and second magnet (21) magnetism attract mutually.

10. An improved aqueous extraction process of the book-growing benefit old prescription according to any of claims 4 to 9, wherein: the extraction process comprises adding 12 times of water, soaking for 2 hr, extracting for 18min, and extracting for 3 times.